阅读说明：本技术 一种润肠通便的益生菌发酵骆驼乳及其制备方法 (Probiotic fermented camel milk capable of relaxing bowels and preparation method thereof ) 是由 宋锦安 华旭 韩迪 杨宇 马璐瑶 于 2020-06-16 设计创作，主要内容包括：本发明涉及乳制品技术领域,具体地说是一种润肠通便的益生菌发酵骆驼乳及其制备方法,按以下重量取原料：120~125克的骆驼奶粉、10~80克的白砂糖、5~20克的罗汉果浓缩汁、0.03~0.08克的酸奶发酵剂、775~865克的纯净水。酸奶发酵剂为长双歧杆菌BL-G301、短双歧杆菌BB-G95、动物双歧杆菌乳亚种BL-G101、两歧双歧杆菌BB-G90、婴儿双歧杆菌BI-G201、青春双歧杆菌BQ-G66中的一种或一种以上的组合物。本发明同现有技术相比,利用六株益生菌中的一种或几种组合发酵,并添加植物提取物罗汉果浓缩汁,发酵成品中含有大于10<Sup>8</Sup>cfu/g的双歧杆菌,产生短链脂肪酸和短链有机酸,从而降低肠道的PH值,能够润肠通便,罗汉果浓缩汁可改善肠燥便秘,益生菌与罗汉果浓缩汁有机结合,共同起到润肠通便的作用。(The invention relates to the technical field of dairy products, in particular to probiotic fermented camel milk for relaxing bowel and a preparation method thereof, wherein the raw materials are taken according to the following weight: 120-125 g of camel milk powder, 10-80 g of white granulated sugar, 5-20 g of fructus momordicae concentrated juice, 0.03-0.08 g of yoghourt leavening agent and 775-865 g of purified water. The yogurt starter is one or more of Bifidobacterium longum BL-G301, Bifidobacterium breve BB-G95, Bifidobacterium animalis subspecies lactis BL-G101, Bifidobacterium bifidum BB-G90, Bifidobacterium infantis BI-G201, and Bifidobacterium adolescentis BQ-G66. Compared with the prior art, the invention utilizes one or more of six probiotics for combined fermentation, and adds plant extract momordica grosvenori concentrated juice, and the content of the fermented finished product is more than 10 8 cfu/g Bifidobacterium produces short chain fatty acids and short chain organic acids to lower intestinal pH and relax bowel, and fructus Siraitiae Grosvenorii concentrated juice can improve intestinal drynessConstipation, the probiotics and the fructus momordicae concentrated juice are organically combined to play a role in relaxing bowel together.)

1. The probiotic fermented camel milk capable of relaxing bowels is characterized by being prepared from 120-125G of camel milk powder, 10-80G of white granulated sugar, 5-20G of fructus momordicae concentrated juice, 0.03-0.08G of yoghourt leavening agent and 775-865G of purified water, wherein the yoghourt leavening agent is one or more of bifidobacterium longum BL-G301, bifidobacterium breve BB-G95, bifidobacterium animalis subspecies BL-G101, bifidobacterium bifidum BB-G90, bifidobacterium infantis BI-G201 and bifidobacterium adolescentis BQ-G66, and the number of viable bacteria of each strain in the yoghourt leavening agent is more than or equal to 1 × 10¹¹cfu／g。

2. The probiotic fermented camel milk capable of relaxing bowels according to claim 1, is characterized in that: the classification name of the Bifidobacterium longum BL-G301 is Bifidobacterium longum BL-G301 (Bifidobacterium longum BL-G301), and the preservation places are as follows: china center for type culture Collection, the collection numbers are: CCTCC NO: m2013689, the preservation time is as follows: 12 and 23 in 2013.

3. The probiotic fermented camel milk capable of relaxing bowels according to claim 1, is characterized in that: the Bifidobacterium breve BB-G95 is classified as Bifidobacterium breve BB-G95 (Bifidobacterium breve BB-G95), and the preservation sites are as follows: china center for type culture Collection, the collection numbers are: CCTCC NO: m2013687, the preservation time is as follows: 12 and 23 in 2013.

4. The probiotic fermented camel milk capable of relaxing bowels according to claim 1, is characterized in that: the classification name of the bifidobacterium animalis subsp lactis BL-G101 is bifidobacterium animalis subsp BL-G101 (Bifidobacterium lactis BL-G101), and the storage places are as follows: china center for type culture Collection, the collection numbers are: CCTCC NO: m2013336, the preservation time is: 7 and 16 in 2013.

5. The probiotic fermented camel milk capable of relaxing bowels according to claim 1, is characterized in that: the classification name of the Bifidobacterium bifidum BB-G90 is Bifidobacterium bifidum BB-G90 (Bifidobacterium bifidum BB-G90), and the preservation sites are as follows: china center for type culture Collection, the collection numbers are: CCTCC NO: m2013194, the preservation time is as follows: year 2013, 5 month and 9 days.

6. The probiotic fermented camel milk capable of relaxing bowels according to claim 1, is characterized in that: the classification name of the Bifidobacterium infantis BI-G201 is Bifidobacterium infantis BI-G201 (Bifidobacterium infantis BI-G201), and the storage places are as follows: china center for type culture Collection, the collection numbers are: CCTCC NO: m2013688, the preservation time is as follows: 12 and 23 in 2013.

7. The probiotic fermented camel milk capable of relaxing bowels according to claim 1, is characterized in that: the classification name of the Bifidobacterium adolescentis BQ-G66 is Bifidobacterium adolescentis BQ-G66 (Bifidobacterium adolescentis BQ-G66), and the preservation sites are as follows: china center for type culture Collection, the collection numbers are: CCTCC NO: m2016167, preservation time: 2016, 4 months and 1 day.

8. The preparation method of the probiotic fermented camel milk for relaxing bowel according to claim 1, which is characterized by comprising the following steps: the preparation method comprises the following steps: step 1, heating 700 g of purified water to 40-45 ℃, uniformly stirring camel milk powder and white granulated sugar, slowly adding the camel milk powder and the white granulated sugar into the purified water while stirring, adding the momordica grosvenori concentrated juice, adding the balance of purified water, fixing the volume to 1000 g, and stirring for 15-20 minutes at the speed of 800-1000 rpm by using a high-speed stirrer to form dissolved feed liquid; step 2, heating the dissolved feed liquid to 65-70 ℃, and homogenizing under the pressure of 20 MPa; step 3, sterilizing the homogenized solution for 10 minutes at the temperature of 115 ℃; step 4, cooling the sterilized solution to 37 ℃, adding a yoghourt starter in an aseptic environment, stirring and dispersing uniformly, filling and sealing; and 5, fermenting at 37 ℃ until the pH value reaches 4.5 +/-0.1, and stopping fermentation to form the fermented camel milk.

Technical Field

The invention relates to the technical field of dairy products, in particular to probiotic fermented camel milk capable of relaxing bowel and a preparation method thereof.

Background

The camel milk is called desert platinum, is easy to digest and absorb, and can invigorate the stomach, thus being an ideal animal edible drink. The protein content in camel milk is higher than that of milk and reaches more than 4 percent, and the milk fat content is also as high as 6 percent. The camel milk contains higher calorie than milk, the calorie of the camel milk is 108.8 kcal, the calorie of the milk is 162 kcal, and the camel milk contains rich vitamins B1, B2 and C and enough nutritional components such as calcium, iron, zinc and the like. Because camel milk does not contain lactoglobulin, allergy can not be caused, and camel milk and processed products thereof are good substitutes for people who have milk allergy. The demand of camel milk and products thereof is much higher than the supply quantity, the activity of popularizing camel milk and products thereof in some countries is developed by food and agriculture organization of united nations at present, the camel milk industry becomes an industry with considerable prospect in Somali, Kenya and other countries, in China, the camel milk producing area is mainly concentrated in inner Mongolia and Xinjiang, and the camel milk and the products thereof are more and more accepted by people along with the pursuit of health and the deep knowledge of camel milk.

In order to further expand camel milk products, a probiotic fermented camel milk capable of relaxing bowel and a preparation method thereof are designed.

Disclosure of Invention

The invention aims to overcome the defects of the prior art and provides probiotic fermented camel milk capable of relaxing bowel and a preparation method thereof.

In order to achieve the purpose, the invention relates to probiotic fermented camel milk capable of relaxing bowel, which is prepared from the following raw materials in parts by weight: 120-125 g of camel milk powder, 10-80 g of white granulated sugar, 5-20 g of fructus momordicae concentrated juice, 0.03-0.08 g of yoghourt leavening agent and 775-865 g of purified water. The yogurt starter comprises Bifidobacterium longum BL-G301, Bifidobacterium breve BB-G95, Bifidobacterium animalis subspecies lactis BL-G101, and Bifidobacterium bifidumBB-G90, Bifidobacterium infantis BI-G201, and Bifidobacterium adolescentis BQ-G66, wherein the number of viable bacteria per strain in yogurt starter is 1 × 10¹¹cfu／g。

The classification name of the bifidobacterium longum BL-G301 is bifidobacterium longum BL-G301 (Bifidobacterium longum BL-G301), and the storage place is as follows: china center for type culture Collection, the collection numbers are: CCTCC NO: m2013689, the preservation time is as follows: 12 and 23 in 2013.

The classification name of the Bifidobacterium breve BB-G95 is Bifidobacterium breve BB-G95 (Bifidobacterium breve BB-G95), and the preservation sites are as follows: china center for type culture Collection, the collection numbers are: CCTCC NO: m2013687, the preservation time is as follows: 12 and 23 in 2013.

The classification name of the Bifidobacterium animalis subsp lactis BL-G101 is Bifidobacterium animalis subsp BL-G101, and the storage places are as follows: china center for type culture Collection, the collection numbers are: CCTCC NO: m2013336, the preservation time is: 7 and 16 in 2013.

The classification name of the bifidobacterium bifidum BB-G90 is bifidobacterium bifidum BB-G90 (Bifidobacterium bifidum BB-G90), and the preservation places are as follows: china center for type culture Collection, the collection numbers are: CCTCC NO: m2013194, the preservation time is as follows: year 2013, 5 month and 9 days.

The classification name of the Bifidobacterium infantis BI-G201 is Bifidobacterium infantis BI-G201 (Bifidobacterium infantis BI-G201), and the storage places are as follows: china center for type culture Collection, the collection numbers are: CCTCC NO: m2013688, the preservation time is as follows: 12 and 23 in 2013.

The classification name of the bifidobacterium adolescentis BQ-G66 is bifidobacterium adolescentis BQ-G66 (Bifidobacterium adolescentis BQ-G66), and the preservation places are as follows: china center for type culture Collection, the collection numbers are: CCTCC NO: m2016167, preservation time: 2016, 4 months and 1 day.

The preparation method of the probiotic fermented camel milk for relaxing bowel comprises the following steps: step 1, heating 700 g of purified water to 40-45 ℃, uniformly stirring camel milk powder and white granulated sugar, slowly adding the camel milk powder and the white granulated sugar into the purified water while stirring, adding the momordica grosvenori concentrated juice, adding the balance of purified water, fixing the volume to 1000 g, and stirring for 15-20 minutes at the speed of 800-1000 rpm by using a high-speed stirrer to form dissolved feed liquid; step 2, heating the dissolved feed liquid to 65-70 ℃, and homogenizing under the pressure of 20 MPa; step 3, sterilizing the homogenized solution for 10 minutes at the temperature of 115 ℃; step 4, cooling the sterilized solution to 37 ℃, adding a yoghourt starter in an aseptic environment, stirring and dispersing uniformly, filling and sealing; and 5, fermenting at 37 ℃ until the pH value reaches 4.5 +/-0.1, and stopping fermentation to form the fermented camel milk.

Compared with the prior art, the invention utilizes one or more of six probiotics for combined fermentation, and adds plant extract momordica grosvenori concentrated juice, and the content of the fermented finished product is more than 10⁸cfu/g of bifidobacterium generates short-chain fatty acid and short-chain organic acid, so that the pH value of the intestinal tract is reduced, the bowel relaxing effect is realized, the momordica grosvenori condensed juice can improve the constipation caused by intestinal dryness, and the probiotics and the momordica grosvenori condensed juice are organically combined to play a role in relaxing the bowel together.

Detailed Description

The invention will now be further described.

The classification name of the Bifidobacterium longum BL-G301 adopted in the invention is Bifidobacterium longum BL-G301 (Bifidobacterium longum BL-G301), and the preservation places are as follows: china center for type culture Collection, the collection numbers are: CCTCC NO: m2013689, the preservation time is as follows: 12 and 23 in 2013.

The Bifidobacterium breve BB-G95 is classified as Bifidobacterium breve BB-G95 (Bifidobacterium breve BB-G95), and the deposit sites are: china center for type culture Collection, the collection numbers are: CCTCC NO: m2013687, the preservation time is as follows: 12 and 23 in 2013.

The classification name of the Bifidobacterium animalis subsp lactis BL-G101 is Bifidobacterium animalis subsp lactis BL-G101, and the storage places are as follows: china center for type culture Collection, the collection numbers are: CCTCC NO: m2013336, the preservation time is: 7 and 16 in 2013.

The classification name of the bifidobacterium bifidum BB-G90 is bifidobacterium bifidum BB-G90 (Bifidobacterium bifidum BB-G90), and the preservation sites are as follows: china center for type culture Collection, the collection numbers are: CCTCC NO: m2013194, the preservation time is as follows: year 2013, 5 month and 9 days.

The classification name of the Bifidobacterium infantis BI-G201 is Bifidobacterium infantis BI-G201 (Bifidobacterium infantis BI-G201), and the storage places are: china center for type culture Collection, the collection numbers are: CCTCC NO: m2013688, the preservation time is as follows: 12 and 23 in 2013.

The classification name of the bifidobacterium adolescentis BQ-G66 is bifidobacterium adolescentis BQ-G66 (Bifidobacterium adolescentis BQ-G66), and the preservation sites are as follows: china center for type culture Collection, the collection numbers are: CCTCC NO: m2016167, preservation time: 2016, 4 months and 1 day.

The invention relates to a probiotic fermented camel milk capable of relaxing bowels, which is prepared from 120-125G of camel milk powder, 10-80G of white granulated sugar, 5-20G of fructus momordicae concentrated juice, 0.03-0.08G of yogurt starter and 775-865G of purified water, wherein the yogurt starter is one or more of bifidobacterium longum BL-G301, bifidobacterium breve BB-G95, bifidobacterium animalis subspecies of lactobacillus BL-G101, bifidobacterium bifidum BB-G90, bifidobacterium infantis BI-G201 and bifidobacterium adolescentis BQ-G66, and the number of viable bacteria of each strain in the yogurt starter is more than or equal to 1 × 10¹¹cfu／g。

The preparation method of the probiotic fermented camel milk for relaxing bowel comprises the following steps: step 1, heating 700 g of purified water to 40-45 ℃, uniformly stirring camel milk powder and white granulated sugar, slowly adding the camel milk powder and the white granulated sugar into the purified water while stirring, adding the momordica grosvenori concentrated juice, adding the balance of purified water, fixing the volume to 1000 g, and stirring for 15-20 minutes at the speed of 800-1000 rpm by using a high-speed stirrer to form dissolved feed liquid; step 2, heating the dissolved feed liquid to 65-70 ℃, and homogenizing under the pressure of 20 MPa; step 3, sterilizing the homogenized solution for 10 minutes at the temperature of 115 ℃; step 4, cooling the sterilized solution to 37 ℃, adding a yoghourt starter in an aseptic environment, stirring and dispersing uniformly, filling and sealing; and 5, fermenting at 37 ℃ until the pH value reaches 4.5 +/-0.1, and stopping fermentation to form the fermented camel milk.

The invention can increase a large amount of live bifidobacterium in the fermented camel milk finished product, and the number of the live bifidobacterium in the finished product yoghourt can reach 10⁸Above cfu/ml, the six strains can tolerate the high acidity of the stomach and the high permeability of bile, survive in the intestinal tract and generate short-chain fatty acid and short-chain organic acid, thereby reducing the pH value of the intestinal tract, promoting the intestinal tract peristalsis, relaxing bowel and improving constipation. The fructus momordicae belongs to the genus of fructus momordicae of the cucurbitaceae family, and is cool in nature, sweet in taste, nontoxic, capable of entering lung and large intestine channels and capable of relaxing bowels in traditional Chinese medicine. It has been commonly used for treating constipation due to intestinal dryness. The literature data prove that the momordica grosvenori aqueous extract has the functions of promoting defecation of normal mice and constipation mice and bidirectionally regulating intestinal movement. The probiotics and the momordica grosvenori concentrated juice are organically combined to play a role in relaxing bowels.