阅读说明：本技术 一种抑制α-葡萄糖苷酶活性羊栖菜制品制备方法和羊栖菜制品 (Preparation method of sargassum fusiforme product for inhibiting activity of alpha-glucosidase and sargassum fusiforme product ) 是由 杨海龙 周化斌 于 2020-06-29 设计创作，主要内容包括：本发明公开了一种抑制α-葡萄糖苷酶活性羊栖菜制品制备方法和羊栖菜制品,该制备方法为以保藏编号为CGMCC5856的蛹虫草菌为制备专用菌,以羊栖菜为原料,通过发酵方式制备获得。经蛹虫草发酵的羊栖菜抑制α-葡萄糖苷酶活性可以提高50.85％-62.71％。(The invention discloses a preparation method of a sargassum fusiforme product for inhibiting activity of alpha-glucosidase and the sargassum fusiforme product, wherein the preparation method is to take cordyceps militaris with the preservation number of CGMCC5856 as a special preparation bacterium and take sargassum fusiforme as a raw material to prepare the sargassum fusiforme product in a fermentation mode. The activity of the sargassum fusiforme fermented by cordyceps militaris for inhibiting the activity of alpha-glucosidase can be improved by 50.85-62.71%.)

1. A preparation method of sargassum fusiforme products for inhibiting activity of alpha-glucosidase is characterized in that: cordyceps militaris with the preservation number of CGMCC5856 is taken as special preparation bacteria, and sargassum fusiforme is taken as a raw material and is prepared by fermentation.

2. The method for preparing sargassum fusiforme product capable of inhibiting alpha-glucosidase activity according to claim 2, wherein the method comprises the following steps:

(1) seed culture: inoculating the cordyceps militaris to a soybean sprout culture medium, and performing rotary type submerged fermentation for 5-7 days at the temperature of 23-25 ℃ and the rotating speed of 140-; the culture medium comprises soybean sprout 200g/L, glucose 20-30g/L, peptone 1-3g/L, and corn flour 3-5 g/L;

(2) fermentation: pulverizing dried Cyrtymenia Sparsa, sieving with 60-80 mesh sieve, weighing 100g Cyrtymenia Sparsa powder, placing in a fermentation container, adding 80-100mL distilled water, stirring, and sterilizing at 121 deg.C for 30 min; cooling, inoculating 10-15% Cordyceps militaris seed solution, stirring, and standing at 25-28 deg.C for fermentation for 6-12 days.

3. A hizikia fusiforme preparation for inhibiting the activity of alpha-glucosidase prepared by the preparation method of claim 1.

Technical Field

The invention relates to the field of microbial fermentation and the field of diabetes health-care food, in particular to a preparation method of a sargassum fusiforme product for inhibiting activity of alpha-glucosidase and the sargassum fusiforme product.

Background

With the rapid development of social economy in China, the life style of people is changed, and the disease spectrum of the world is also changed. The incidence of chronic non-infectious diseases such as hypertension, diabetes, coronary heart disease and the like is gradually increased, and the chronic non-infectious diseases tend to be younger and seriously harm the health of human beings. Among them, Diabetes (Diabetes mellitis) has become one of the diseases with the fastest rate of prevalence in our country, and the summary of the 8 th version of Diabetes map published by the International Diabetes Federation (IDF) shows: in 2017, adult diabetic patients (20-79 years old) have 4.25 million people in the world, 1/4, the majority of Chinese people, is the most diabetic countries, and the number is estimated to increase to 6.42 million by 2040 years, while Chinese diabetic patients reach 1.51 million (thin phoenix, Chenyan, 2019. research progress on the correlation of intestinal flora regulation and type 2 diabetes mellitus. Shanghai preventive medicine, 31(3): 242-. Postprandial hyperglycemia is one of the important symptoms of type II diabetes mellitus, alpha-glucosidase catalyzes hydrolysis of oligosaccharides such as sucrose, maltose and the like in the small intestine to further cause increase of blood sugar in vivo, and currently, representative drugs clinically used for inhibiting activity of the alpha-glucosidase comprise acarbose (acarbose), fuglibose (voglibose), miglitol (miglitol) and the like, and have good curative effect, however, long-term use of the polysaccharide can cause side effects such as liver and kidney injury and digestive tract reaction of a human body (Ninghao 281564m, Sun 24354m, Xianpengquine, soup is beautiful, Wangzhonghua, Wangcheng, Chenhaimin, Qianjiang, 2019. inhibition effect of sargassum fusiforme component polysaccharide on alpha-glucosidase, nuclear agriculture report, 33(2): 0297-.

Sargassum fusiforme (Sargassum fusiforme) is a marine vegetable and medicinal seaweed in japan, china, korea and korean coastal areas. In China, Hizikia fusiforme has a long history of entering medicine, pharmacopoeias such as Ben Cao gang mu and Shen nong Ben Cao Jing are recorded, and modern pharmacological research shows that the Hizikia fusiforme has the efficacies of enhancing the immunity of organisms, delaying senility, reducing blood sugar, reducing blood fat and the like (Xiehejejie, Huiyou, Shenting, Lodangshuang, Yangying, Nanhai content, 2014, research progress of chemical components and pharmacological activity of the Hizikia fusiforme, Zhejiang agriculture science, (4) 487 491).

However, the conventional methods for processing sargassum fusiforme are all edible by using cooked cold sauce, and the capability of inhibiting the activity of alpha-glucosidase is not strong, so that the improvement is needed.

Disclosure of Invention

In order to solve the problems and the defects of the prior art, the invention aims to provide a preparation method of a sargassum fusiforme product for inhibiting the activity of alpha-glucosidase and the sargassum fusiforme product. The Sargassum fusiforme fermented by the cordyceps militaris of the special strain can improve the capability of inhibiting the activity of alpha-glucosidase, so that the prepared Sargassum fusiforme has the health-care function of preventing diabetes.

In order to achieve the above objects, the first aspect of the present invention provides a method for preparing hizikia fusiforme product capable of inhibiting alpha-glucosidase activity.

In order to realize the purpose, the technical scheme is that the cordyceps militaris with the preservation number of CGMCC5856 is taken as special preparation bacteria, and the sargassum fusiforme is taken as a raw material and is prepared by a fermentation mode.

The method is further provided with the following steps:

(1) seed culture: inoculating the cordyceps militaris to a soybean sprout culture medium, and performing rotary type submerged fermentation for 5-7 days at the temperature of 23-25 ℃ and the rotating speed of 140-; the culture medium comprises soybean sprout 200g/L, glucose 20-30g/L, peptone 1-3g/L, and corn flour 3-5 g/L;

(2) fermentation: pulverizing dried Cyrtymenia Sparsa, sieving with 60-80 mesh sieve, weighing 100g Cyrtymenia Sparsa powder, placing in a fermentation container, adding 80-100mL distilled water, stirring, and sterilizing at 121 deg.C for 30 min; cooling, inoculating 10-15% Cordyceps militaris seed solution, stirring, and standing at 25-28 deg.C for fermentation for 6-12 days.

The second purpose of the invention is to provide a sargassum fusiforme product capable of inhibiting the activity of alpha-glucosidase, prepared by the preparation method.

The invention has the beneficial effects that:

the method has simple process and lower cost, can obviously improve the activity of the sargassum fusiforme for inhibiting the alpha-glucosidase after being treated by the method, and can improve the activity of the sargassum fusiforme for inhibiting the alpha-glucosidase by 50.85-62.71 percent according to embodiment detection.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention will be further described in detail with reference to the following examples.