阅读说明：本技术 一种基于生物蛋白的信息存储方法 (Information storage method based on biological protein ) 是由 陶虎 周志涛 于 2020-06-04 设计创作，主要内容包括：本发明涉及信息存储领域,具体是一种基于生物蛋白的信息存储方法,所述方法包括：S1.制备掺杂或未掺杂功能基团的生物蛋白膜；S2.对待存储信息进行编码；S3.将编码后的信息以及功能基团中的信息存储于所述生物蛋白膜中；本发明能够存储生物信息,能够原位“写入”和“读取”数字化信息,能够实现数字化信息的重复写入或擦除,同时能够耐受高温、高湿度、辐照、磁场等恶劣条件。(The invention relates to the field of information storage, in particular to an information storage method based on biological protein, which comprises the following steps: s1, preparing a biological protein film doped or undoped with functional groups; s2, coding information to be stored; s3, storing the coded information and the information in the functional groups in the biological protein membrane; the invention can store biological information, can write in and read digital information in situ, can realize repeated writing or erasing of the digital information, and can resist severe conditions such as high temperature, high humidity, irradiation, magnetic field and the like.)

1. A method for storing information based on biological protein is characterized by comprising the following steps:

s1, preparing a biological protein film doped or undoped with functional groups;

s2, coding information to be stored;

and S3, storing the coded information and the information in the functional groups in the biological protein membrane.

2. The bioprotein-based information storage method of claim 1, wherein the step S1 comprises the steps of:

s11, evaporating a layer of metal material on a substrate;

s12, spin-coating a layer of biological protein solution on the substrate with the metal material evaporated to form a biological protein film.

3. The bioprotein-based information storage method of claim 2,

the substrate is Si, GaAs, AlN or Al₂O₃、ITO、SiO₂SiN, glass material; the biological protein is one of silk fibroin, sericin, spidroin, deer antler protein, egg white protein and collagen.

4. The method for storing information based on biological protein as claimed in claim 2, wherein the biological protein solution can be doped with functional groups in advance, and the functional groups are one or more of biomarkers, DNA, quantum dots, antibiotics, nanoparticles, growth factors, proteases and antibodies.

5. The method for storing information based on biological protein as claimed in claim 4, wherein the biological protein film is incorporated with biomarker and DNA to store biologically relevant information.

6. The method for storing information based on bioprotein of claim 4, wherein said bioprotein membrane is antibacterial after being incorporated with antibiotics.

7. The method for storing biological protein-based information according to claim 4, wherein the stored information can be controllably degraded after the incorporation of protease into the biological protein film.

8. The bioprotein-based information storage method of claim 1, wherein step S2 comprises:

and coding the information to be stored according to a preset coding form.

9. The bioprotein-based information storage method of claim 1, wherein the step S3 comprises the steps of:

s31, radiating a needle point of the atomic force microscope by adopting a laser source with preset power, wherein the radiation frequency of the laser source corresponds to the absorption spectrum of the biological protein film;

and S32, moving the biological protein film, and directly writing a dot matrix structure corresponding to the coded information on the biological protein film.

10. The bioprotein-based information storage method of claim 9, wherein after step S32, the method further comprises:

decoding the information stored in the biological protein film, and reading the information stored in the biological protein film.

11. The bioprotein-based information storage method of claim 10,

the decoding information stored in the bioprotein membrane, reading information stored in the bioprotein membrane comprising:

and scanning the lattice structure by adopting an atomic force microscope, decoding the information in the lattice structure, and reading the information in the lattice structure.

12. The bioprotein-based information storage method of claim 9, wherein after step S32, the method further comprises:

and erasing the information stored in the biological protein film, and rewriting the information stored in the biological protein film.

13. The bioprotein-based information storage method of claim 12,

the erasing the information stored in the bioprotein membrane, and the rewriting the information stored in the bioprotein membrane includes:

the needle point of the atomic force microscope is radiated by an infrared laser source with the power less than or greater than the preset power to eliminate the lattice structure,

and adopting the infrared laser source with the preset power to radiate the needle point of the atomic force microscope to rewrite the lattice structure.

Technical Field

The invention relates to the field of information storage, in particular to an information storage method based on biological protein.

Background

Over the past two decades, information storage has developed a number of strategies including: deep or extreme ultraviolet light sources, dual beam systems and 3D storage architectures are used to increase the optical storage density to hundreds of Gb/inch 2. However, to achieve high spatial resolution, many of these methods inevitably involve complex processes that are time and cost inefficient. Furthermore, they use conventional optics that are limited by diffraction limits and do not improve storage density well above current industry standards.

Scattering-type scanning near-field optical microscopy, originally used to achieve super-resolution imaging beyond the diffraction limit, offers a promising alternative strategy for facilitating high-resolution nanofabrication. The use of near-field electromagnetic interactions between optical media (e.g., photosensitive substrates or nanoparticles) and extreme sub-wavelength scale incident light paves the way for nanofabrication and manipulation using photo-induced effects. For example, optical fibers or scanning metal probes (or probe arrays) with ultra-sharp tips can be used to induce nonlinear optical phenomena by high optical energy density of evanescent fields. The evanescent field can be used for processing a nanoscale region on the surface of a material, manufacturing an optical nanometer device and performing nanometer photoetching.

In order to increase the lithography efficiency and thus the efficiency of the information storage process, the dielectric material, the wavelength of the incident light and the tip size and material of the probe must be considered cooperatively. In particular, the lithographic modes in the medium (including light-induced, heat-induced, electrically-induced and stress/strain-induced phase transitions) are actually material dependent. In this case, silk fibroin, a naturally occurring protein from silkworms, is widely appreciated for its mechanical strength, optical transparency, biocompatibility, biodegradability and adjustable water solubility. Since this material can undergo radiation-induced nano-scale polymorphic transformations, it has been used as a resist-thin layer for transferring circuit patterns to semiconductor substrates by electron beam, ion beam lithography. However, these existing lithographic methods typically operate under high vacuum or rely on mask-based transfer methods.

In addition, in the information age of today, the forms of information are diversified, and the recording and storing methods of information are continuously improved. However, limited to information storage media, current information storages are mainly used to store physical information, and are not suitable for storing bio-based information with activity (bio-active) that varies with time.

Silk fibroin is also a biological material with good biocompatibility, easy doping and functionalization and easy nano processing, and the silk fibroin is used as a medium for information storage, so that the storage of physical information (namely, the coded data is stored through a surface nano structure) can be realized; in the future, the information of the living body can be stored and analyzed through the interaction between the living body and the silk fibroin.

However, how to utilize silk fibroin to store information is urgently needed to be solved by those skilled in the art.

Disclosure of Invention

In view of the above problems in the prior art, an object of the present invention is to provide an information storage method based on biological proteins, which can store biological information, can "write" and "read" digitized information in situ, can realize repeated writing or erasing of the digitized information, and can withstand severe conditions such as high temperature, high humidity, irradiation, and magnetic field.

In order to solve the above problems, the present invention provides a method for storing information based on bioprotein, comprising the steps of:

s1, preparing a biological protein film doped or undoped with functional groups;

s2, coding information to be stored;

and S3, storing the coded information and the information in the functional groups in the biological protein membrane.

Further, step S1 includes the steps of:

s11, evaporating a layer of metal material on a substrate;

s12, spin-coating a layer of biological protein solution on the substrate with the metal material evaporated to form a biological protein film.

Further, the substrate is Si, GaAs, AlN, Al₂O₃、ITO、SiO₂SiN, glass material;

the biological protein is one of silk fibroin, sericin, spidroin, deer antler protein, egg white protein and collagen.

Further, the biological protein solution may be doped with a functional group in advance, and the functional group is one or more of a biomarker, DNA, a quantum dot, an antibiotic, a nanoparticle, a growth factor, a protease, and an antibody.

Furthermore, after the biological protein membrane is doped with a biomarker and DNA, biologically relevant information can be stored.

Further, the biological protein film can be antibacterial after being doped with antibiotics.

Further, after protease is doped into the biological protein film, the stored information can be controllably degraded.

Further, step S2 includes:

and coding the information to be stored according to a preset coding form.

Further, step S3 includes the steps of:

s31, radiating a needle point of the atomic force microscope by adopting a laser source with preset power, wherein the radiation frequency of the laser source corresponds to the absorption spectrum of the biological protein film;

and S32, moving the biological protein film, and directly writing a dot matrix structure corresponding to the coded information on the biological protein film.

Further, after step S32, the method further includes:

decoding the information stored in the biological protein film, and reading the information stored in the biological protein film.

Further, the decoding the information stored in the bioprotein membrane, reading the information stored in the bioprotein membrane comprising:

and scanning the lattice structure by adopting an atomic force microscope, decoding the information in the lattice structure, and reading the information in the lattice structure.

Further, after step S32, the method further includes:

and erasing the information stored in the biological protein film, and rewriting the information stored in the biological protein film.

Further, the erasing the information stored in the bioprotein membrane, and the rewriting the information stored in the bioprotein membrane includes:

the needle point of the atomic force microscope is radiated by an infrared laser source with the power less than or greater than the preset power to eliminate the lattice structure,

and adopting the infrared laser source with the preset power to radiate the needle point of the atomic force microscope to rewrite the lattice structure.

Due to the technical scheme, the invention has the following beneficial effects:

the information storage method based on the bioprotein can store biological information, can write in and read digital information in situ, can realize repeated writing or erasing of the digital information, and can resist severe conditions such as high temperature, high humidity, irradiation, magnetic field and the like.

Drawings

In order to more clearly illustrate the technical solution of the present invention, the drawings used in the description of the embodiment or the prior art will be briefly described below. It is obvious that the drawings in the following description are only some embodiments of the invention, and that for a person skilled in the art, other drawings can be derived from them without inventive effort.

FIG. 1 is a flow chart of a method for storing information based on biological proteins according to an embodiment of the present invention;

fig. 2 is a flowchart of step S1 provided by the embodiment of the present invention;

fig. 3 is a flowchart of step S3 provided by the embodiment of the present invention;

FIG. 4 is a schematic diagram of a lattice structure according to an embodiment of the present invention;

FIG. 5 is a partial schematic view of a lattice structure according to an embodiment of the present invention;

FIG. 6 is a schematic diagram of a lattice structure provided in the second embodiment of the present invention;

fig. 7 is a schematic diagram of a lattice structure with elimination processing provided by the second embodiment of the present invention.

FIG. 8 is a graph comparing normalized activity of a biological protein membrane incorporating biomarkers and DNA provided by embodiments of the present invention;

FIG. 9 is a graph comparing the amount of information on a biological protein membrane incorporating a biomarker and DNA provided by an example of the present invention;

FIG. 10 is a graph comparing the antimicrobial properties of a bioprotein film incorporating antibiotics provided by examples of the present invention.

FIG. 11 is a graph comparing the degradation performance of protease-incorporated bioprotein films provided by examples of the present invention;

FIG. 12 is a graph comparing other properties of the bioprotein membranes provided by the examples of the present invention.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be obtained by a person skilled in the art without any inventive step based on the embodiments of the present invention, are within the scope of the present invention.

Reference herein to "one embodiment" or "an embodiment" means that a particular feature, structure, or characteristic may be included in at least one implementation of the invention. In the description of the present invention, it is to be understood that the terms "upper", "lower", and the like, indicate orientations or positional relationships based on those shown in the drawings, are merely for convenience in describing the present invention and simplifying the description, and do not indicate or imply that the referred device or element must have a specific orientation, be constructed in a specific orientation, and be operated, and thus, should not be construed as limiting the present invention.