阅读说明：本技术 磷脂浓缩物制造方法 (Process for producing phospholipid concentrate ) 是由 汤浅浩气 川村纯 琴浦聪 于 2019-02-20 设计创作，主要内容包括：本发明提供一种方法,在使家畜或家禽组织的乙醇提取液浓缩物接受脱胶工序、回收胶质而获得磷脂浓缩物时,能够抑制每次该操作时所得的磷脂量的波动。更具体而言,提供一种方法,是由家畜或家禽肉生产磷脂浓缩物的方法,该方法包括：(A)工序,将家畜或家禽的组织的乙醇提取液浓缩物与水混合,并且相对于所述浓缩物100质量份混合小于7质量份的水；以及(B)工序,将所得的混合液在2℃以下离心分离。(The present invention provides a method for suppressing fluctuation of the amount of phospholipids obtained in each operation when a phospholipid concentrate is obtained by subjecting an ethanol extract concentrate of livestock or poultry tissues to a degumming step and recovering the pectin. More specifically, there is provided a method for producing a phospholipid concentrate from livestock or poultry meat, the method comprising: (A) mixing an ethanol extract concentrate of a tissue of a livestock or poultry with water, and mixing less than 7 parts by mass of water per 100 parts by mass of the concentrate; and (B) centrifuging the resulting mixture at 2 ℃ or lower.)

1. A method of producing a phospholipid concentrate from tissue of livestock or poultry comprising:

a step (A) in which a concentrate of an ethanol extract solution of a tissue of a livestock or poultry is mixed with water, and water is mixed in an amount of less than 7 parts by mass per 100 parts by mass of the concentrate; and

and (B) centrifuging the resulting mixture at 2 ℃ or lower.

2. The method of claim 1, wherein,

the water content of the ethanol extract concentrate of the tissue of livestock or poultry is 1% by mass or less.

3. A method of producing a phospholipid concentrate from tissue of livestock or poultry comprising:

a1 step of concentrating an ethanol extract of livestock or poultry tissue;

a2 step of mixing the concentrate with water and mixing less than 7 parts by mass of water per 100 parts by mass of the concentrate; and

and (B) centrifuging the resulting mixture at 2 ℃ or lower.

4. The method of claim 3, wherein,

concentrating the ethanol extract of the tissue of livestock or poultry until the water content of the obtained concentrated solution is 1 mass% or less.

5. The method according to claim 3 or 4, further comprising a0 step of extracting livestock or poultry tissue with ethanol,

when the precipitation occurs in this step, the precipitation is dissolved by heating, or the step is performed at a temperature at which the precipitation does not occur.

6. The method according to any one of claims 1 to 5, further comprising a step C of recovering a precipitate generated by the centrifugal separation.

7. A method for concentrating phospholipids contained in tissues of livestock or poultry, comprising:

a step (A) in which a concentrate of an ethanol extract of a tissue of a livestock or poultry is mixed with water, and water is mixed in an amount of less than 7 parts by mass per 100 parts by mass of the concentrate; and

and (B) centrifuging the resulting mixture at 2 ℃ or lower.

8. The method according to any one of claims 1 to 7,

the tissue of livestock or poultry is the tissue of birds.

Technical Field

The present invention relates to a method for producing a phospholipid concentrate.

Background

Phospholipids are important as components of living bodies, and particularly, for example, plasmalogens are attracting attention as functional materials having various effects such as memory enhancement. Therefore, a method for efficiently producing phospholipids is desired.

Methods for extracting components such as phospholipids from poultry breast meat by ethanol extraction have been developed.

Disclosure of Invention

Problems to be solved by the invention

Attempts have been made to concentrate phospholipids, which are one of the components considered to be particularly important, after extracting useful components from tissues of livestock and poultry with ethanol. In general, in the case of oil purification, an extract from an animal or plant is subjected to a degumming step to remove a gum containing phospholipids as a main component. As described above, since phospholipids are mainly components contained in the pectin, in order to efficiently concentrate phospholipids, it has been studied to concentrate ethanol extracts of livestock and poultry tissues, subject them to a degumming step, and recover the pectin.

Although this method enables concentration of phospholipids, it was investigated that the amount of phospholipids obtained varies every time concentration is performed. Since it is important to make the quality uniform when a phospholipid concentrate is supplied to the market, it is necessary to develop a method in which the quality (particularly, the amount of phospholipid) does not fluctuate every time the concentration operation is performed.

An object of the present invention is to provide a method for obtaining a phospholipid concentrate by subjecting an ethanol extract concentrate of livestock or poultry tissues to a degumming step and recovering a gum, which method can suppress fluctuations in the amount of phospholipids obtained each time the operation is performed.

Means for solving the problems

The present inventors have found that the treatment schedule in the degumming step and the conditions of the gum recovery step are further repeatedly improved, and have completed the present invention. As described above, in the present invention, since the phospholipid is concentrated, the gum is recovered in the degumming step. Therefore, it is considered that the process is also called a degumming process, and more specifically, a gum recovery process. Therefore, in the present specification, the degumming step is hereinafter referred to as a "gum recovery step".

The present invention includes, for example, the main spool described in the following items.

A method for producing a phospholipid concentrate from a tissue of livestock or poultry, or a method for concentrating phospholipids contained in a tissue of livestock or poultry, the method comprising:

(A) mixing an ethanol extract concentrate of a tissue of a livestock or poultry with water, and mixing less than 7 parts by mass of water per 100 parts by mass of the concentrate; and

(B) and (3) centrifuging the resulting mixture at 2 ℃ or lower.

The method according to the item 1, wherein the water content of the ethanol extract concentrate of the tissue of the livestock or poultry is 1% by mass or less.

A method for producing a phospholipid concentrate from or concentrating phospholipids contained in livestock or poultry tissues, the method comprising:

(a1) concentrating an ethanol extract of a tissue of a livestock or poultry;

(a2) a step of mixing the obtained concentrate with water, and mixing less than 7 parts by mass of water with respect to 100 parts by mass of the concentrate; and

(B) and (3) centrifuging the resulting mixture at 2 ℃ or lower.

The method according to item 3, wherein the ethanol extract of the tissue of the livestock or poultry is concentrated until the water content of the resulting concentrated solution is 1 mass% or less.

Item 5. the method according to item 3 or 4, further comprising a step (a0) of extracting the livestock or poultry tissue with ethanol. When the precipitation occurs in this step, the precipitation is first dissolved by heating, or the step is performed at a temperature at which the precipitation does not occur.

The method according to any one of the items 1 to 5, further comprising a step (C) of recovering a precipitate generated by the centrifugal separation.

Item 7. a method for concentrating phospholipids contained in tissues of livestock or poultry, which comprises:

(A) mixing a concentrate of an ethanol extract of a tissue of a livestock or poultry with water, and mixing less than 7 parts by mass of water per 100 parts by mass of the concentrate; and

(B) and (3) centrifuging the resulting mixture at 2 ℃ or lower.

The method according to any one of items 1 to 7, wherein the tissue of the livestock or poultry is a tissue of birds.

Effects of the invention

According to the method of the present invention, when a phospholipid concentrate is obtained by subjecting an ethanol extract concentrate of livestock or poultry tissues to a degumming step and recovering a gum, fluctuation in the amount of phospholipids obtained each time the concentration is performed can be suppressed.

Drawings

FIG. 1 shows the results of examining by TLC whether the amount of phospholipid in the upper layer has changed or not depending on the difference in the amount of water in the ethanol extract concentrate added in the gel recovery step.

Fig. 2 shows the results of examining whether or not the appearance of the precipitate obtained when the centrifugal separation conditions were changed in the gum recovery step was changed.

Detailed Description

Hereinafter, embodiments of the present invention will be described in further detail.

The method for producing a phospholipid concentrate from livestock or poultry tissue (hereinafter sometimes referred to as "the phospholipid concentrate production method of the present invention") included in the present invention includes: (A) mixing an ethanol extract concentrate of a tissue of a livestock or poultry with water, and mixing less than 7 parts by mass of water per 100 parts by mass of the concentrate; and (B) centrifuging the resulting mixture at 2 ℃ or lower.

Examples of livestock and poultry include cattle, pigs, horses, sheep, goats, and birds. In the case of mammals, the tissue containing phospholipids includes mainly skin, brain, intestine, heart, genitals, meat (particularly muscle), and the like, and phospholipids can be extracted from these tissues. Further, the birds include chickens, domestic ducks, quails, wild ducks, pheasants, turkeys and the like. In view of ease of acquisition, cost, and a feeling of resistance to taste, chicken is particularly preferable. The bird tissue is not particularly limited, and for example, bird meat (particularly, bird breast meat), bird skin, and bird entrails are preferable. It is to be noted that 2 or more different tissues of 1 or more organisms may be combined. Particularly preferred examples of livestock and poultry tissues include breast meat of birds, and particularly preferred is breast meat of chickens.

It is noted that the livestock or poultry tissue may be cut to the appropriate size. Alternatively, it may be dried (particularly freeze-dried) tissue of livestock or poultry.

The method for ethanol extraction of livestock or poultry tissues is not particularly limited, and a known method or a method which can be easily matched with a known method can be used. For example, the method can be carried out by adding ethanol to the tissues of livestock or poultry in a mass ratio of about 1 to 5 times, and stirring or leaving the mixture to stand. Stirring or standing may also be carried out with heating. The heating may be performed at about 30 to 50 ℃ or about 35 to 45 ℃. The time for stirring or standing is not particularly limited, and may be, for example, about 0.5 to 24 hours or about 1 to 12 hours. The obtained extract can be subjected to solid-liquid separation by filtration or the like as required. Further, the same operation may be performed on the extraction residue to obtain an extraction liquid again, and the total amount may be added to the previously obtained extraction liquid.

In addition, when the temperature is low (particularly in winter), precipitation may occur in the extraction step. Such a temperature is not limited as long as precipitation occurs, and specific examples thereof include 10 ℃ or lower, 9 ℃ or lower, 8 ℃ or lower, 7 ℃ or lower, 6 ℃ or lower, 5 ℃ or lower, 4 ℃ or lower, 3 ℃ or lower, 2 ℃ or lower, 1 ℃ or lower, and 0 ℃ or lower. Since the phospholipid is contained in the precipitate, if the ethanol extraction operation is continued regardless of the precipitate, the phospholipid contained in the precipitate is not contained in the ethanol extract, and therefore the amount of phospholipid contained in the finally obtained phospholipid concentrate may fluctuate. Therefore, when the deposition occurs, it is preferable to first heat the solution to dissolve the deposition or to perform the step at a temperature at which the deposition does not occur. When the precipitates are dissolved by heating, the temperature of heating is not particularly limited as long as the precipitates are dissolved and the quality is not affected, and may be, for example, about 20 to 30 ℃. In addition, in the case of performing the ethanol extraction step at a temperature at which precipitation does not occur, the step may be performed at a temperature of, for example, about 20 to 30 ℃.

The method for concentrating the obtained ethanol extract is not particularly limited, and a known method or a method that can be easily matched with a known method can be used. For example, concentration under reduced pressure or concentration by heating may be mentioned.

The concentration is preferably carried out until the water content of the obtained ethanol extract concentrate becomes 1 mass% or less, more preferably 0.9 mass% or less, 0.8 mass% or less, 0.7 mass% or less, 0.6 mass% or less, or 0.5 mass% or less, and still more preferably 0.4 mass% or less, 0.3 mass% or less, or 0.2 mass% or less. The moisture content is a value obtained by the karl fischer method.

The ethanol content of the obtained ethanol extract concentrate is preferably 15% by mass or less, and more preferably 14% by mass or less, 13% by mass or less, 12% by mass or less, 11% by mass or less, 10% by mass or less, 9% by mass or less, or 8% by mass or less. The ethanol content is a value obtained by subtracting the moisture content from the loss on drying obtained by the dry heat drying method (105 ℃ C., 3 hours). For example, when the dry heat drying loss is 90 mass% and the water content is 1 mass%, the ethanol content is 100-90-1 to 9 (mass%).

The amount of water mixed with the ethanol extract concentrate is less than 7 parts by mass with respect to 100 parts by mass of the concentrate. Preferably 6.9 parts by mass or less, 6.8 parts by mass or less, 6.7 parts by mass or less, 6.6 parts by mass or less, 6.5 parts by mass or less, 6.4 parts by mass or less, 6.3 parts by mass or less, 6.2 parts by mass or less, 6.1 parts by mass or less, or 6 parts by mass or less. The lower limit of the mixing ratio of water is not particularly limited as long as the effect of the present invention is not impaired, and is, for example, preferably 0.5 parts by mass or more, more preferably 1 part by mass or more, and still more preferably 1.5 parts by mass or more, per 100 parts by mass of the concentrate.

The mixture of the ethanol extract concentrate and water was centrifuged to recover the resulting precipitate (lower layer). At this time, the centrifugal separation is carried out at a temperature of 2 ℃ or lower. Preferably at 1 ℃ or lower, more preferably at 0.5 ℃ or lower. The temperature for centrifugation is not particularly limited as long as the concentrate can be centrifuged without freezing, and examples thereof include-2 ℃ or higher, -1.5 ℃ or higher, -1 ℃ or higher, and-0.5 ℃ or higher.

The recovery of the precipitate (lower layer) can be carried out, for example, by removing the liquid portion (upper layer). This removal can be carried out by a known method, for example, decantation, but is preferably carried out by suction.

The recovered precipitate can be used directly as a phospholipid concentrate. The recovered precipitate may be heated (e.g., about 35 to 45 ℃) and stirred to be dissolved for use. The stirring here is preferably carried out under a stream of nitrogen.

Further, the present invention also includes a method for concentrating phospholipids contained in tissues of livestock or poultry, the method comprising: (A) mixing a concentrate of an ethanol extract of a tissue of a livestock or poultry with water, and mixing less than 7 parts by mass of water per 100 parts by mass of the concentrate; and (B) centrifuging the resulting mixture at 2 ℃ or lower. Since this method can be performed through the same steps as the above-described method for producing a phospholipid concentrate, the description of the above-described method for producing a phospholipid concentrate is also directly applicable to the method for concentrating the phospholipid.

In the present specification, the term "comprising" includes "consisting essentially of" and "consisting of" ("comprising" includes "contained addressing of" and "contained of.").