阅读说明：本技术 一种葛根植物提取物的萃取工艺 (Extraction process of kudzu root plant extract ) 是由 叶荣棣 于 2020-07-15 设计创作，主要内容包括：本发明涉及葛根植物的提取工艺,更具体地说,它涉及一种葛根植物提取物的萃取工艺,其技术方案要点是：S1、提供葛根干片；S2、将葛根干片和纯水混合,开始加热回流提取,制得一级葛根粗液,并排出残留渣；S3、将一级葛根粗液进行过滤,获得葛根浊液；S4、将残留渣进行二次提取,获得二级葛根粗液,对二级葛根粗液进行过滤,获得葛根浊液；S5、将葛根浊液静置,获得上清液；S6、将上清液进行一级膜分离,以获得一级浓缩清液；S7、将一级浓缩清液进行二级膜分离,取浓水,获得浓缩基液；S8、将浓缩基液进行浓缩,以获得浓缩浸膏；S9、将浓缩浸膏进行干燥脱水,并冷冻,以获得葛根提取物。本发明具有精准率高、能靶向有效萃取、节约能耗的优点。(The invention relates to an extraction process of a pueraria plant, in particular to an extraction process of a pueraria plant extract, which has the technical scheme that: s1, providing dried kudzu vine root slices; s2, mixing the dried radix Puerariae slices with pure water, heating and reflux-extracting to obtain first-grade radix Puerariae crude liquid, and discharging the residue; s3, filtering the primary kudzu root crude liquid to obtain a kudzu root turbid liquid; s4, carrying out secondary extraction on the residual slag to obtain a secondary radix puerariae crude liquid, and filtering the secondary radix puerariae crude liquid to obtain a radix puerariae turbid liquid; s5, standing the turbid radix puerariae solution to obtain a supernatant; s6, performing primary membrane separation on the supernatant to obtain primary concentrated clear liquid; s7, carrying out secondary membrane separation on the primary concentrated clear liquid, and taking concentrated water to obtain a concentrated base liquid; s8, concentrating the concentrated base solution to obtain a concentrated extract; s9, drying and dehydrating the concentrated extract, and freezing to obtain the kudzu root extract. The invention has the advantages of high precision, effective extraction in a targeted way and energy consumption saving.)

1. The extraction process of the kudzu root plant extract is characterized by comprising the following steps:

s1, providing dried kudzu vine root slices;

s2, mixing the dried radix puerariae slices with pure water, heating and refluxing for extraction to obtain a primary radix puerariae crude liquid, and discharging residual slag;

s3, filtering the primary kudzu root crude liquid to obtain a kudzu root turbid liquid;

s4, carrying out secondary extraction on the residual slag to obtain a secondary radix puerariae crude liquid, and filtering the secondary radix puerariae crude liquid to obtain the radix puerariae turbid liquid;

s5, standing the turbid radix puerariae solution to obtain a supernatant;

s6, performing primary membrane separation on the supernatant to obtain primary concentrated clear liquid;

s7, carrying out secondary membrane separation on the primary concentrated clear liquid, and taking concentrated water to obtain a concentrated base liquid;

s8, concentrating the concentrated base solution to obtain a concentrated extract;

s9, drying and dehydrating the concentrated extract, and freezing to obtain the kudzu root extract.

2. The extraction process of pueraria lobata plant extract according to claim 1, wherein the step S1 further comprises the steps of:

and (3) removing branches and mud of the radix puerariae after the radix puerariae is unearthed, and cleaning, drying and slicing the radix puerariae to obtain the dried radix puerariae slices.

3. The extraction process of pueraria lobata plant extract according to claim 1, further comprising the following steps before step S2:

and (3) soaking the dried kudzuvine root slices in clear water for 2 hours.

4. The extraction process of pueraria lobata plant extract according to claim 1, wherein the extraction process comprises the steps of: the dried kudzu root slices and the pure water are mixed according to the mass ratio of 1: 6.

5. The extraction process of pueraria lobata plant extract according to claim 1, wherein the extraction process comprises the steps of: the heating reflux extraction time in the step S2 is 3h, and the materials are stirred in stages and/or stirred by tumbling with pressure air in the heating reflux extraction process.

6. The extraction process of pueraria lobata plant extract according to claim 1, wherein the extraction process comprises the steps of: the water addition amount of the secondary extraction in the step S4 is 70% of that of the step S2, and the extraction time is 2 h.

7. The extraction process of pueraria lobata plant extract according to claim 1, wherein the extraction process comprises the steps of: the standing time of the turbid radix puerariae liquid is 2 hours.

8. The extraction process of pueraria lobata plant extract according to claim 1, wherein the extraction process comprises the steps of: the concentration conditions of the concentrated base liquid are that the temperature is less than 68 ℃, the vacuum degree is 0.04-0.06 Mpa, and the feed liquid concentration of the concentrated base liquid is 1.15-1.25 wave ratio.

9. The extraction process of pueraria lobata plant extract according to claim 1, wherein the extraction process comprises the steps of: the freezing temperature of the concentrated extract is 86 ℃ below zero, and the water content of the concentrated extract is less than or equal to 3%.

Technical Field

The invention relates to an extraction process of a pueraria plant, in particular to an extraction process of a pueraria plant extract.

Background

The radix Puerariae extract is dried root extract of Pueraria lobata Ohwi, Pueraria lobata Ohwi or Pueraria thomsonii of Leguminosae, and mainly contains flavonoid such as daidzin, daidzein, puerarin, 7-glucoside and puerarin-7-xyloside. Puerarin has effects of dilating coronary artery and cerebral artery, lowering blood pressure, slowing heart rate, reducing oxygen consumption of myocardium, relieving symptoms of hypertension and coronary heart disease such as dizziness, headache, and neck rigidity, and recovering and enhancing memory. The pueraria isoflavone has the effects of resisting cancers, preventing arteriosclerosis, improving osteoporosis and the like, regulates the balance of estrogen in vivo, maintains the second sexual characteristics of women and keeps the root of youth, and is superior to other phytoestrogens in that the pueraria isoflavone is not an endless estrogen supplement and has a bidirectional regulation function, so that the pueraria isoflavone does not have the symptom of excessive estrogen after being eaten. The safety is the best of the phytoestrogen products.

At present, the main extraction process of the pueraria extract comprises the following steps: (1) selecting dried radix Puerariae, and cleaning; crushing into coarse particles or slicing, decocting with water for 2 times (3 hr or 2 hr respectively), mixing the two filtrates, standing for 2 hr, and filtering to obtain radix Puerariae extractive solution; (2) concentrating the radix puerariae extracting solution at the vacuum degree of 0.06-0.08 Mpa to the relative density of 1.10-1.15 (70-80 ℃) to obtain concentrated thick extract, carrying out spray drying on the thick extract (the inlet temperature is 180 +/-5 ℃ and the outlet temperature is 70 +/-5 ℃), crushing the dried dry extract into fine powder with the size of 80 meshes, sampling, carrying out qualified standard inspection, and carrying out sealed packaging to obtain the radix puerariae extract, wherein the yield is about 20%.

The technical scheme has the defects that: in the extraction process of the kudzu root extract, the temperature is not controlled, so that other beneficial substances in the kudzu root extract are damaged or lose efficacy, the extraction rate is low, and the economic benefit of an enterprise is greatly reduced.

Disclosure of Invention

Aiming at the defects in the prior art, the invention aims to provide the extraction process of the pueraria lobata plant extract, which can prevent the pueraria lobata plant from being damaged during extraction, effectively avoid the use of other chemical organic solvents, improve the product safety and have the advantages of high precision, high extraction rate, targeted and effective extraction and energy consumption saving.

The technical purpose of the invention is realized by the following technical scheme, and the extraction process of the pueraria lobata plant extract comprises the following steps:

s1, providing dried kudzu vine root slices;

s2, mixing the dried radix puerariae slices with pure water, and performing heating reflux extraction to obtain a first-level radix puerariae crude liquid and a residue;

s3, filtering the primary kudzu root crude liquid to obtain a kudzu root turbid liquid;

s4, carrying out secondary extraction on the residual slag to obtain a secondary radix puerariae crude liquid, and filtering the secondary radix puerariae crude liquid to obtain the radix puerariae turbid liquid;

s5, standing the turbid radix puerariae solution to obtain a supernatant;

s6, performing primary membrane separation on the supernatant to obtain primary concentrated clear liquid;

s7, carrying out secondary membrane separation on the primary concentrated clear liquid, and taking concentrated water to obtain a concentrated base liquid;

s8, concentrating the concentrated base solution to obtain a concentrated extract;

s9, drying and dehydrating the concentrated extract, and freezing to obtain the kudzu root extract.

In one embodiment, step S1 further includes the following steps:

and (3) removing branches and mud of the radix puerariae after the radix puerariae is unearthed, and cleaning, drying and slicing the radix puerariae to obtain the dried radix puerariae slices.

In one embodiment, step S2 is preceded by the following steps:

and (3) soaking the dried kudzuvine root slices in clear water for 2 hours.

In one embodiment, the dried radix puerariae slices and the pure water are mixed in a mass ratio of 1: 6.

In one embodiment, the heating reflux extraction time in step S2 is 3 hours, and the stirring and/or the tumbling of the pressurized air is performed in stages during the heating reflux extraction.

In one embodiment, the added water amount in the secondary extraction in the step S4 is 70% of that in the step S2, and the extraction time is 2 h.

In one embodiment, the standing time of the radix puerariae turbid liquid is 2 hours.

In one embodiment, the concentration condition of the concentrated base liquid is that the temperature is less than 68 ℃, the vacuum degree is 0.04-0.06 Mpa, and the feed liquid concentration of the concentrated base liquid is 1.15-1.25 wave ratio.

In one embodiment, the freezing temperature of the concentrated extract is 86 ℃ below zero, and the water content of the concentrated extract is less than or equal to 3%.

The extraction process of the pueraria plant extract has the following beneficial effects:

firstly, the extraction rate is high, and in the extraction process of the dried kudzu root slices, pure water is adopted for mixing and heating reflux extraction, so that the substances in the kudzu root can be protected to fully enter the kudzu root crude liquid through distillation/reflux of the pure water, and a concentrated extract rich in beneficial substances is generated in the subsequent process;

secondly, in order to prevent beneficial substances in the residual slag from being not extracted in the primary extraction, secondary extraction and secondary filtration are adopted, so that the effective rate of extraction is improved to a greater extent, and the beneficial substances in the kudzuvine root are prevented from being lost and causing waste;

thirdly, the operation is simple, other organic chemical solvents are not mixed in the whole extraction process, and the product safety is improved;

fourthly, the extraction precision is high, the molecular weights of different membranes are utilized to separate (concentrate) so as to obtain precise substances, and the energy consumption is greatly saved.

Drawings

FIG. 1 is a schematic flow chart of the extraction process of this embodiment.

Detailed Description

The invention is described in detail below with reference to the figures and examples.

Referring to fig. 1, an extraction process of pueraria lobata plant extract includes the following steps:

s1, providing the dried kudzu root tablets.

Furthermore, the kudzu vine root is removed from the earth, branches and mud are removed, and the kudzu vine root is cleaned, dried and sliced to obtain the kudzu vine root dry slice.

S2, mixing the dried radix Puerariae slices with pure water, heating and reflux-extracting to obtain first-grade radix Puerariae crude liquid, and discharging the residue.

Specifically, before the dried radix puerariae pieces are heated and refluxed for extraction, the dried radix puerariae pieces need to be soaked in clear water for 2 hours, so that beneficial substances in the dried radix puerariae pieces can be effectively discharged into primary radix puerariae crude liquid through heating. In the embodiment, dried radix puerariae slices are placed into an extraction tank to be heated and refluxed for extraction, the dried radix puerariae slices and pure water in the extraction tank are mixed according to the mass ratio of 1:6, after all materials are put into the extraction tank, the extraction tank is started to be heated by steam to begin primary extraction, a reflux device is started at the same time, in the primary extraction process, the temperature and the pressure in the extraction tank are adjusted, when the temperature in the extraction tank rises to be higher than 95 ℃, the pressure in the extraction tank is stabilized and the steam consumption is adjusted, after primary extraction lasts for 3 hours, a steam switch is turned off, and materials in the extraction tank are stirred and/or stirred by pressure air in stages in the primary extraction process.

S3, filtering the primary kudzu root crude liquid to obtain the kudzu root turbid liquid.

Specifically, after once extracting, open the discharge gate of extracting the jar, discharge one-level root of kudzu vine thick liquid, simultaneously, open the feed inlet of filter to introduce one-level root of kudzu vine thick liquid and carry out filtering work in the filter. In this embodiment, the filter is provided with two, and two filters used alternately, filter completely to accomplish until one-level root of kudzu vine crude liquor, prevent to mix the impurity in the one-level root of kudzu vine crude liquor into the root of kudzu vine turbid liquid.

S4, extracting the residue for the second time to obtain a second-level radix puerariae crude liquid, and filtering the second-level radix puerariae crude liquid to obtain a radix puerariae turbid liquid.

In order to prevent the residual slag in the extraction tank from being incompletely extracted, the residual slag is subjected to secondary extraction. And repeating the steps of once extraction for the pure water of the residual slag in the extraction tank to carry out heating reflux extraction, and filtering the discharged secondary coarse kudzu vine root liquid to finally obtain the turbid kudzu vine root liquid. The water addition amount of the secondary extraction is 70% of that of the primary extraction, and the extraction time of the secondary extraction is 2 h.

S5, standing the turbid liquid of the kudzuvine root to obtain a supernatant.

Specifically, the standing time of the turbid radix puerariae liquid is 2 hours, and when impurities in the turbid radix puerariae liquid are precipitated to a lower layer, a supernatant of the turbid radix puerariae liquid is obtained.

And S6, performing primary membrane separation on the supernatant to obtain primary concentrated clear liquid.

And (3) extracting the supernatant into a ceramic membrane separation device (the pore diameter of the ceramic membrane is larger than the molecular structure of the flavonoid compound), separating the supernatant by the ceramic membrane separation device, and discharging primary concentrated clear liquid.

And S7, carrying out secondary membrane separation on the primary concentrated clear liquid, and taking concentrated water to obtain a concentrated base liquid.

And extracting the primary extracting solution to a polyphenol separating device, and discharging the concentrated base solution.

Specifically, through the steps of primary membrane separation and secondary membrane separation, macromolecular structural substances such as starch, plant fibers, proteins and the like which are included in the extracting solution are effectively separated, and other substances which are included in the extracting solution and have molecular weights smaller than flavonoid compounds are effectively separated. The user utilizes the molecular weights of different membranes to separate (concentrate) so as to obtain accurate substances, achieve the target effective extraction of the kudzuvine root and save energy consumption.

S8, concentrating the concentrated base solution to obtain a concentrated extract.

And extracting the concentrated base solution to a two-effect concentration unit for concentration, wherein the concentration conditions are set to be that the temperature is less than 68 ℃, the vacuum degree is 0.04-0.06 MPa, and the feed solution concentration of the concentrated base solution is 1.15-1.25 wave ratio.

S9, drying and dehydrating the concentrated extract, and freezing to obtain the kudzu root extract.

And (3) putting the concentrated extract into a freeze drying unit for drying and dehydrating, wherein the freezing temperature is set to 86 ℃ so as to obtain a final kudzu root extract, and the water content of the kudzu root extract is lower than 3%.

The above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the present invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention. Therefore, the protection scope of the present patent shall be subject to the appended claims.