阅读说明：本技术 一种抗关节炎的食品 (Anti-arthritis food ) 是由 杜琪珍 徐龙杰 张海华 金鹏 张亚辉 于 2021-06-16 设计创作，主要内容包括：本发明提供一种抗关节炎的食品,以EGCG和软骨素为主要的配伍原料,添加乳清蛋白作为载体,通过超声纳米化技术制备成纳米包合物。本食品经动物实验证实对缓解关节炎有较好的效果,可长期食用,相对于药物来说,风险少。(The invention provides an anti-arthritis food, which is a nano inclusion compound prepared by using EGCG and chondroitin as main compatible raw materials and adding lactalbumin as a carrier through an ultrasonic nano technology. Animal experiments prove that the food has a good effect of relieving arthritis, can be eaten for a long time, and has less risk compared with medicaments.)

1. An anti-arthritis food is characterized in that EGCG and chondroitin are used as main compatible raw materials, lactalbumin is added as a carrier, and a nano inclusion compound is prepared by an ultrasonic nano technology, wherein the preparation method comprises the following steps: dissolving EGCG, chondroitin and lactalbumin with water at room temperature, preparing into nanometer clathrate by ultrasonic nanometer technology, spray drying into powder, and making into granule.

2. The anti-arthritic food according to claim 1, wherein the EGCG, chondroitin and whey protein are present in a weight of 1g, 1g and 8g, respectively.

3. The anti-arthritis food according to claim 1, wherein said EGCG is natural EGCG extracted from tea leaves, and the purity is 90-99%.

4. The anti-arthritic food according to claim 1, wherein said food is at a temperature of 10 to 25 ℃ at room temperature; the spraying temperature is 50-60 ℃.

Technical Field

The invention relates to the field of health-care food, and mainly relates to anti-arthritis food and a preparation method thereof.

Background

Arthritis is a disease closely related to a plurality of factors such as bacteria, viruses, sex hormones, heredity and the like, and the main symptoms are divided into two aspects of joint expression and extraarticular expression. Arthritis is slow in onset and is manifested by symptoms of fatigue, weakness, weight loss, gastrointestinal discomfort, mild fever, numbness and tingling of hands and feet and the like, and joint bone stiffness is easy to cause if the joint bone stiffness is not cured in time. In addition to joint damage, arthritis is also highly likely to cause peripheral complications such as rheumatoid vasculitis, rheumatoid heart and lung disease, kidney damage, and the like. Arthritis has become an important disease affecting human health, and if the disease is not aggravated, the disease can lead to repeated attacks, thus seriously affecting the physical health of patients.

Cartilage powder (ChS) is a substance containing active protein and chondroitin sulfate and can be used in health food field. The cartilage powder has multiple activities, such as lysozyme activity, cell growth promoting activity, protease inhibiting activity, etc., and the viscous polysaccharide contained in the cartilage powder can promote and enhance human immunity. However, the cartilage powder has poor heat resistance, and the heat in the processing process can cause the cartilage powder to lose the biological activity. On the other hand, the absorption of the cartilage powder by the human body influences the biological activity of the cartilage powder, and the cartilage powder with smaller particle size is more beneficial to the absorption of the human body, thereby promoting the cartilage powder to fully exert the biological activity. However, because chondroitin sulfate is not suitable for the field of food, a green and efficient chondroitin utilization method is more needed.

Epigallocatechin gallate (EGCG), which is the main component of green tea polyphenols, is a catechin monomer separated from tea leaves. EGCG has strong scavenging ability to free radicals, has very good antioxidant activity, and can protect cells.

Disclosure of Invention

The invention aims to provide an anti-arthritis food, aiming at relieving the harm of arthritis to human body.

In order to achieve the purpose, the invention adopts the following technical scheme:

an anti-arthritis food, which takes EGCG and chondroitin as main compatible raw materials, is added with lactalbumin as a carrier, and is prepared into a nano clathrate compound by an ultrasonic nano technology, and the preparation method comprises the following steps: dissolving EGCG, chondroitin and lactalbumin with water at room temperature, preparing into nanometer clathrate by ultrasonic nanometer technology, spray drying into powder, and making into granule.

Preferably, the weight of the EGCG, the chondroitin and the lactalbumin is 1g, 1g and 8g respectively.

Preferably, the EGCG is natural EGCG extracted from tea leaves, and the purity of the EGCG is 90-99%.

Preferably, the room temperature is 10-25 ℃; the spraying temperature is 50-60 ℃.

The dose of the anti-arthritis food is 1 time per day and 1 granule per time, which is taken by adults.

Through a large number of experiments, EGCG and chondroitin are combined according to the proportion relation, and the whey protein with the proportion relation is added, so that the damage of stress to HFLS-OA and HFLS-RA cells can be effectively prevented, and the damage of joint structures can be reduced. Therefore, the anti-arthritis food is an anti-arthritis food with great development potential, can resist joint swelling, and has certain prevention and adjuvant therapy effects on joint lesions. Our studies show that the EGCG and the chondroitin are combined according to the proportion of the invention, and have stronger anti-arthritis effect than the EGCG and the chondroitin which are used independently, and the lactalbumin (WP) not only supplements protein required by human bodies, but also can reduce the gastrointestinal burden and enhance the function of immunity.

Arthritis belongs to chronic diseases, and clinically, non-steroidal anti-inflammatory drugs are commonly used, the drugs have quick response but short maintenance time, joint structure destruction can be induced by long-term administration, and the risk of eating anti-arthritis food is relatively low. Animal experiments prove that the EGC-ChS-WP anti-arthritis food developed by the research has better effect on relieving arthritis, and further has good social benefit and economic benefit expected by popularization and application.

Detailed Description

The following describes embodiments of the present invention in further detail with reference to the accompanying drawings.

The EGCG used in the examples is natural EGCG extracted from tea: food grade EGCG (purity 90-99%) purchased from Hangzhou Qinyuan natural plant limited company; chondroitin: food-grade chondroitin purchased from Jiangsu Jiayuan biological science and technology Limited company and with the mass content of 90 percent.

Example 1

An anti-arthritis food contains EGCG, chondroitin and lactalbumin in an amount of 1g, 1g and 8g respectively. The preparation method comprises the following steps: dissolving 10kg of EGCG, 10kg of chondroitin and 80kg of casein by using water at room temperature, adding the dissolved EGCG and chondroitin solution into a whey protein solution, fully and uniformly stirring, preparing a nano inclusion compound by using an ultrasonic nano technology, and then spraying (50-60 ℃) to dry into powder (which can pass through a 100-mesh sieve) to prepare particles, wherein each particle is 10g in weight.

Test 1, EGCG-chondroitin-whey protein food particle (EGC-ChS) inhibits SD rat arthritis test determination:

an SD rat arthritis model (the animal production license number is SCXK (Zhe) 2019-:

a: a normal group; b: a model group CK; c: celecoxib administration group (CEL); d: glucosamine administration Group (GA); e: anti-arthritis food administration group of example 1 (ChS)

The results of the inflammation inhibition results obtained by 9-month administration of four groups b, c, d, and e are shown in fig. 1:

the detection steps are as follows:

1. and (5) establishing an arthritis model. Bovine type II collagen was injected subcutaneously into the right hindpaw of the foot in an amount of 0.2mL to induce arthritis, followed by two immunizations.

2. And (5) performing intragastric administration. According to the conversion coefficient of the dose per kilogram of the body weight of the animals and the human bodies, the concentration is calculated, the anti-arthritis health care product is prepared, and the anti-arthritis health care product is administrated to the tested rats in a gastric lavage administration mode.

3. The inflammation score was evaluated. Inflammation scores were observed and evaluated once per week.

Finally, it should also be noted that the above-mentioned list is only a few specific embodiments of the present invention. It is obvious that the invention is not limited to the above embodiments, but that many variations are possible. All modifications which can be derived or suggested by a person skilled in the art from the disclosure of the present invention are to be considered within the scope of the invention.