阅读说明：本技术 一种含酶抑菌剂的中药发酵饲料及其制备方法 (Traditional Chinese medicine fermented feed containing enzyme bacteriostat and preparation method thereof ) 是由 胡晓歌 李雪刚 王旭光 于 2020-06-11 设计创作，主要内容包括：本发明公开了一种含酶抑菌剂的中药发酵饲料,主要由以下质量的原料组成：酶抑菌剂0.2-1.5kg,纤维素酶0.5-1kg、木聚糖酶0.5-1kg、发酵载体60-150kg,酸奶饮料1-5kg,鲜牛奶0-5kg、饲料载体80-200kg、葡萄糖2-4kg,酿酒酵母3-5kg,粪肠球菌3-6kg,地衣3-8kg。本发明还提供了所述含酶抑菌剂的中药发酵饲料的制备方法。本发明的中药发酵饲料含有酶抑菌剂,可以优化家畜肠道环境,提高食欲,增加采食量,有利于家畜体内产生抗菌物质,提升家畜抗病能力。(The invention discloses a traditional Chinese medicine fermented feed containing an enzyme bacteriostatic agent, which mainly comprises the following raw materials by mass: 0.2-1.5kg of enzyme bacteriostatic agent, 0.5-1kg of cellulase, 0.5-1kg of xylanase, 60-150kg of fermentation carrier, 1-5kg of yoghurt beverage, 0-5kg of fresh milk, 80-200kg of feed carrier, 2-4kg of glucose, 3-5kg of saccharomyces cerevisiae, 3-6kg of enterococcus faecalis and 3-8kg of lichen. The invention also provides a preparation method of the traditional Chinese medicine fermented feed containing the enzyme bacteriostatic agent. The traditional Chinese medicine fermented feed contains the enzyme bacteriostatic agent, can optimize the intestinal environment of livestock, improve appetite, increase feed intake, facilitate the production of antibacterial substances in the bodies of the livestock and improve the disease resistance of the livestock.)

1. A traditional Chinese medicine fermented feed containing an enzyme bacteriostatic agent is characterized by mainly comprising the following raw materials by mass: 0.2-1.5kg of enzyme bacteriostatic agent, 0.5-1kg of cellulase, 0.5-1kg of xylanase, 60-150kg of fermentation carrier, 1-5kg of yoghurt beverage, 0-5kg of fresh milk, 80-200kg of feed carrier, 2-4kg of glucose, 3-5kg of saccharomyces cerevisiae, 3-6kg of enterococcus faecalis and 3-8kg of lichen.

2. The traditional Chinese medicine fermented feed containing the enzyme bacteriostatic agent according to claim 1, which is characterized by mainly comprising the following raw materials by mass: 0.5-1kg of enzyme bacteriostat, 0.8-1kg of cellulase, 0.8-1kg of xylanase, 80-120kg of fermentation carrier, 2.5-4kg of yoghurt drink, 2-5kg of fresh milk, 150kg of feed carrier 100, 2.5-3.6kg of glucose, 3.5-4kg of saccharomyces cerevisiae, 3.2-4.6kg of enterococcus faecalis and 3.8-6.8kg of lichen.

3. The fermented traditional Chinese medicine feed containing enzyme bacteriostat according to claim 1 or 2, wherein the enzyme bacteriostat is glucose oxidase.

4. The fermented traditional Chinese medicine feed containing enzyme bacteriostat according to claim 1 or 2, wherein the fermentation carrier is a traditional Chinese medicine composition; the traditional Chinese medicine composition is 10-15kg of liquorice, 8-20kg of epimedium, 6-12kg of astragalus, 6-25kg of semen cuscutae, 5-15kg of bighead atractylodes rhizome, 5-18kg of codonopsis pilosula, 8-25kg of hawthorn and 12-20kg of honeysuckle.

5. The fermented traditional Chinese medicine feed containing the enzyme bacteriostatic agent according to claim 1 or 2, wherein the yoghourt beverage contains lactobacillus bulgaricus, streptococcus thermophilus and lactic acid bacteria.

6. The fermented traditional Chinese medicine feed containing the enzyme bacteriostatic agent according to claim 1 or 2, wherein the feed carrier is one or two of corn and soybean meal.

7. The preparation method of the traditional Chinese medicine fermented feed containing the enzyme bacteriostatic agent according to any one of claims 1 to 6, which is characterized by mainly comprising the following steps:

(1) weighing a certain amount of fresh milk, heating to 65-70 deg.C, adding sufficient purified water, cooling to 25-30 deg.C, adding yogurt, glucose, Saccharomyces cerevisiae, enterococcus faecalis and lichen, stirring, pouring into a sterilized fermentation tank, sealing, and fermenting;

(2) adding a certain amount of glucose and warm water into the fermentation liquor obtained in the step (1), and uniformly stirring to obtain fermentation liquor for later use;

(3) weighing a plurality of fermentation carriers, sequentially performing coarse crushing, bidirectional dynamic countercurrent extraction and solid-liquid separation to obtain water extraction liquid medicine,

(4) and (3) drying the water extraction liquid medicine prepared in the step (3), uniformly mixing the dried solid with the enzyme bacteriostatic agent and the fermentation liquor prepared in the step (2), filling the mixture into a fermentation bag, and fermenting in a sealed fermentation chamber for 5-10 days to obtain the fermented feed.

8. The method for preparing fermented traditional Chinese medicine feed containing enzyme bacteriostat according to claim 7, wherein the specific method of bidirectional dynamic countercurrent extraction adopted in the step (3) is as follows: water is used as an extracting agent for bidirectional dynamic countercurrent extraction to extract the coarsely crushed ancient body fermentation carrier.

9. The method for preparing the fermented traditional Chinese medicine feed containing the enzyme bacteriostatic agent according to claim 8, wherein the bidirectional dynamic countercurrent extraction has a speed of 600-650L/h for the left-side reverse feeding of the leaching agent, a speed of 180-220L/h for the right-side reverse feeding of the leaching agent, a feeding speed of 120-140 kg/h for the solid raw material drug, a screw rod rotation speed of 16-18 rpm/min, and a screw propeller rotation speed of 20-30 rpm/min.

10. The method for preparing fermented traditional Chinese medicine feed containing enzyme bacteriostat according to claim 8 or 9, wherein the temperature of bidirectional dynamic countercurrent extraction is 70-90 ℃ and the extraction time is 7-10 h.

Technical Field

The invention relates to the field of traditional Chinese medicine fermented feed, in particular to traditional Chinese medicine fermented feed containing an enzyme bacteriostatic agent and a preparation method thereof.

Background

The feed is a material basis for the development of the breeding industry and is directly related to the quality safety of livestock products and the improvement of agriculture, rural economic development and the living standard of people. As the breeding industry develops, most farmers progressively recognize the importance of fermented feed in poultry and livestock production. More and more farmers use fermented feed to feed poultry and livestock.

The basic problem that fermented feed on the existing market is easy to go mouldy in summer is that the indoor temperature is higher and the air humidity is higher in summer, so that mould is easy to grow and breed, and after the feed is contacted with air, the mould can be bred quickly, so that the feed is mouldy. Moreover, most fermented feeds in the market are mainly subjected to aerobic fermentation, the safety is poor, the aerobic fermentation is easy to generate the pollution of sundry fungi such as mold and the like, a large amount of heat and gas are generated in the fermentation process, the energy loss of the feeds is serious, and the weight loss of the fermented feeds is about 20 percent or even higher.

In order to inhibit the growth and propagation of the mold, some manufacturers add a certain amount of chemical bacteriostatic agent into the feed, so that although the chemical bacteriostatic agent can inhibit the growth and propagation of the mold, the chemical bacteriostatic agent can also inhibit the propagation of other beneficial bacteria in the feed, and the using effect of the fermented feed is influenced, thereby further influencing the quality of livestock products.

Disclosure of Invention

In order to solve the problems in the prior art, the invention aims to provide a traditional Chinese medicine fermented feed containing an enzyme bacteriostatic agent and a preparation method thereof, which are used for reducing the pH value of the feed, inhibiting the growth and the propagation of harmful bacteria and promoting the rapid proliferation of beneficial bacteria, thereby improving the quality of the feed and prolonging the shelf life.

In order to achieve the purpose, the invention adopts the following technical scheme:

a traditional Chinese medicine fermented feed containing an enzyme bacteriostatic agent mainly comprises the following raw materials by mass: 0.2-1.5kg of enzyme bacteriostatic agent, 0.5-1kg of cellulase, 0.5-1kg of xylanase, 60-150kg of fermentation carrier, 1-5kg of yoghurt beverage, 0-5kg of fresh milk, 80-200kg of feed carrier, 2-4kg of glucose, 3-5kg of saccharomyces cerevisiae, 3-6kg of enterococcus faecalis and 3-8kg of lichen.

Further, the feed mainly comprises the following raw materials by mass: 0.5-1kg of enzyme bacteriostat, 0.8-1kg of cellulase, 0.8-1kg of xylanase, 80-120kg of fermentation carrier, 2.5-4kg of yoghurt drink, 2-5kg of fresh milk, 150kg of feed carrier 100, 2.5-3.6kg of glucose, 3.5-4kg of saccharomyces cerevisiae, 3.2-4.6kg of enterococcus faecalis and 3.8-6.8kg of lichen.

The enzyme bacteriostatic agent is glucose oxidase.

The fermentation carrier is a traditional Chinese medicine composition; the traditional Chinese medicine composition is 10-15kg of liquorice, 8-20kg of epimedium, 6-12kg of astragalus, 6-25kg of semen cuscutae, 5-15kg of bighead atractylodes rhizome, 5-18kg of codonopsis pilosula, 8-25kg of hawthorn and 12-20kg of honeysuckle.

The yoghourt beverage contains lactobacillus bulgaricus, streptococcus thermophilus and lactic acid bacteria.

The feed carrier is one or two of corn and soybean meal.

A preparation method of the traditional Chinese medicine fermented feed containing the enzyme bacteriostatic agent mainly comprises the following steps:

(1) weighing a certain amount of fresh milk, heating to 65-70 deg.C, adding sufficient purified water, cooling to 25-30 deg.C, adding yogurt, glucose, Saccharomyces cerevisiae, enterococcus faecalis and lichen, stirring, pouring into a sterilized fermentation tank, sealing, and fermenting;

(2) adding a certain amount of glucose and warm water into the fermentation liquor obtained in the step (1), and uniformly stirring to obtain fermentation liquor for later use;

(3) weighing a plurality of fermentation carriers, sequentially performing coarse crushing, bidirectional dynamic countercurrent extraction and solid-liquid separation to obtain water extraction liquid medicine,

(4) and (3) drying the water extraction liquid medicine prepared in the step (3), uniformly mixing the dried solid with the enzyme bacteriostatic agent and the fermentation liquor prepared in the step (2), filling the mixture into a fermentation bag, and fermenting in a sealed fermentation chamber for 5-10 days to obtain the fermented feed.

The specific method for bidirectional dynamic countercurrent extraction adopted in the extraction in the step (3) is as follows: water is used as an extracting agent for bidirectional dynamic countercurrent extraction to extract the coarsely crushed ancient body fermentation carrier.

The speed of the left-side reverse-direction leaching agent in the bidirectional dynamic countercurrent extraction is 600-650L/h, the speed of the right-side reverse-direction leaching agent in the bidirectional dynamic countercurrent extraction is 180-220L/h, the feeding speed of the solid raw material medicine is 120-140 kg/h, the rotating speed of a screw rod is 16-18 rpm/min, and the rotating speed of a screw propeller is 20-30 rpm/min.

The temperature of the bidirectional dynamic countercurrent extraction is 70-90 ℃, and the extraction time is 7-10 h.

Compared with the prior art, the invention has the following beneficial effects:

(1) compared with other chemical bacteriostatic agents, the biological enzyme bacteriostatic agent has the advantages that the biological enzyme bacteriostatic agent is higher in safety when being used as a biological agent, and can inhibit the growth and the reproduction of mould and promote the growth and the reproduction of beneficial bacteria when being added into fermented feed.

(2) The biological enzyme bacteriostatic agent has the effects of clearing pathogenic factors in livestock bodies, particularly in intestinal tracts, optimizing the intestinal tract environment, improving appetite, increasing feed intake, replacing antibiotics and probiotics, protecting immune systems, enhancing liver functions, reducing medicine residues in the livestock bodies and improving the quality of livestock products.

(3) The biological enzyme bacteriostatic agent has the functions of quickly relieving toxin poisoning, particularly mycotoxin poisoning, in the prevention and treatment of diseases of livestock; can be used for adjuvant treatment of diseases, and has improved therapeutic effect and accelerated healing.

(4) The traditional Chinese medicine fermentation and the probiotics have synergistic effect, and the antibacterial and immune system capability is improved. After the traditional Chinese medicine fermentation and the probiotics have synergistic effect, antibacterial substances in the traditional Chinese medicine are easier to release, the probiotics are also influenced by the proliferation promoting effect of the traditional Chinese medicine, the breeding speed of growing livestock is increased, the antibacterial substances in bodies of the livestock are more favorably generated, and the disease resistance of the livestock is improved.

Detailed Description

The present invention is further illustrated in detail below with reference to examples, but it should be understood that the scope of the present invention is not limited to the specific embodiments.