阅读说明：本技术 用于病毒载体分离的方法和试剂盒 (Methods and kits for viral vector isolation ) 是由 T.史密斯 于 2018-12-20 设计创作，主要内容包括：本发明涉及用于纯化病毒载体的方法,更具体地,它涉及通过使用单一自动化过程,从生产者细胞中纯化病毒载体。该方法包括下述步骤：a)将生产者细胞和细胞裂解缓冲液加入处理容器中；b)在所述处理容器中混合所述生产者细胞和细胞裂解缓冲液,以获得混合物；c)使所述混合物流过用于纯化病毒载体的色谱柱,其中所述病毒载体被吸附到所述色谱柱上；和d)将病毒载体从色谱柱中洗脱到产物容器内。(The present invention relates to a method for purifying viral vectors, more specifically it relates to the purification of viral vectors from producer cells by using a single automated process. The method comprises the following steps: a) adding the producer cells and a cell lysis buffer to a processing vessel; b) mixing the producer cells and cell lysis buffer in the processing vessel to obtain a mixture; c) flowing the mixture through a chromatography column for purifying a viral vector, wherein the viral vector is adsorbed onto the chromatography column; and d) eluting the viral vector from the chromatography column into a product container.)