阅读说明：本技术 一种白番茄提取物脂质体冻干粉的制备方法及其应用 (Preparation method and application of white tomato extract liposome freeze-dried powder ) 是由 薛泉 于 2020-06-23 设计创作，主要内容包括：本发明公开了一种白番茄提取物脂质体冻干粉的制备方法及其应用,包括白番茄提取物和脂质体膜层,所述脂质体膜层内部包裹白番茄提取物,所述脂质体膜层的组成原料包括氢化饱和磷脂和胆固醇,将白番茄进行超微粉碎、超临界二氧化碳流体萃取,之后再进行脂质体处理,最后冻干形成稳定的、易保存的白番茄提取物脂质体冻干粉；白番茄提取物使用后减少黑色素的生成,使皮肤白皙,防止脱氧核糖核酸被破坏,防止肌肤内胶原蛋白减少,含L-半胱氨酸令美白效果加倍,有效发挥抗氧化和抗发炎功效,保护皮肤免受紫外线的伤害；采用脂质体对白番茄提取物进行包裹,具有靶向性,可以准确的找到受损细胞进行修复,营养成分被装载在脂质球内促进皮下的细胞吸收。(The invention discloses a preparation method and application of white tomato extract liposome freeze-dried powder, which comprises a white tomato extract and a liposome membrane layer, wherein the inside of the liposome membrane layer is wrapped with the white tomato extract, the raw materials of the liposome membrane layer comprise hydrogenated saturated phospholipid and cholesterol, white tomatoes are subjected to superfine grinding and supercritical carbon dioxide fluid extraction, then liposome treatment is carried out, and finally, the white tomato extract liposome freeze-dried powder which is stable and easy to store is formed by freeze-drying; the white tomato extract reduces the generation of melanin after being used, makes the skin white, prevents deoxyribonucleic acid from being damaged, prevents collagen in the skin from being reduced, doubles the whitening effect due to the L-cysteine, effectively plays the effects of oxidation resistance and inflammation resistance, and protects the skin from being damaged by ultraviolet rays; the white tomato extract is wrapped by the liposome, so that targeting is realized, damaged cells can be accurately found for repair, and nutritional ingredients are loaded in the lipid spheres to promote subcutaneous cell absorption.)

1. The white tomato extract liposome freeze-dried powder is characterized by comprising a white tomato extract and a liposome membrane layer, wherein the inside of the liposome membrane layer is wrapped with the white tomato extract, and the raw materials of the liposome membrane layer comprise hydrogenated saturated phospholipid and cholesterol.

2. The lyophilized white tomato extract liposome powder of claim 1, wherein the liposome membrane is further wrapped with a vitamin layer.

3. The white tomato extract liposome lyophilized powder of claim 2, wherein the vitamin layer is composed of hydrophilic vitamin or lipophilic vitamin.

4. The preparation method of the white tomato extract liposome freeze-dried powder as claimed in claim 1, which is characterized by comprising the following steps:

s1, selecting materials, selecting full white tomatoes without damage on surfaces, cleaning and airing for later use;

s2, preparing a white tomato extract, and preparing a nano white tomato extract by adopting low-temperature crushing and supercritical fluid extraction;

s3, preparing liposome, dissolving the liposome raw material in deionized water to prepare liposome aqueous solution;

s4, preparing a white tomato extract liposome, and wrapping the white tomato extract into the liposome by using a film-ultrasonic dispersion method to prepare the nano white tomato extract liposome;

s5, freeze drying, namely freeze drying the white tomato extract liposome to obtain the white tomato extract liposome freeze-dried powder.

5. The method for preparing the white tomato extract liposome lyophilized powder as claimed in claim 4, wherein in S2, the preparation of the white tomato extract comprises the following steps:

a1 slicing white tomato, wherein the thickness of each slice is not more than 0.2 cm;

a2 pulverizing at low temperature, pulverizing white tomato slices with a low-speed pulverizer at a temperature not higher than 12 deg.C to obtain fine particles with a particle size of 80-120 meshes;

a3 constant temperature extraction, mixing white tomato particles and a solvent to prepare a suspension, wherein the mass ratio of the white tomato particles to the solvent is 1: 6-12; atomizing the suspension by using an atomizer, spraying out, naturally settling liquid mist to recover into suspension, collecting the suspension, and keeping the temperature at 4-10 ℃;

a4, filtering and separating, namely filtering and separating the suspension obtained in the step A2 to obtain clear liquid and residues;

a5 secondary leaching, mixing the residue and solvent to obtain suspension, wherein the mass ratio of Acanthopanax trifoliatus particles to solvent is 1: 4-8; atomizing the suspension by using an atomizer, spraying out, naturally settling liquid mist to recover into suspension, collecting the suspension, and keeping the temperature at 4-10 ℃;

a6, secondary filtration, namely filtering the suspension obtained in the step A4 to obtain clear liquid and waste residue;

a7 solvent separating, mixing the clear liquids obtained in step A3 and step A5, heating, and separating the solvent to obtain white tomato extract.

6. The method for preparing the white tomato extract liposome freeze-dried powder as claimed in claim 5, wherein in step A2, the temperature formula is T-10-5 sina, wherein T is the current temperature, and a is the pulverization time in minutes.

7. The method for preparing the lyophilized powder of white tomato extract liposome as claimed in claim 5, wherein in the step A3, the suspension is treated by ultrasonic for 1-3h at 200 KHz and 3-8W/cm ultrasonic intensity²。

8. The use of the lyophilized white tomato extract liposome powder of claim 1, wherein the lyophilized white tomato extract liposome powder is used as a whitening agent in cosmetics.

9. The use of the lyophilized powder of white tomato extract liposome as claimed in claim 8, wherein the cosmetic comprises whitening cosmetic, sunscreen cosmetic, anti-aging cosmetic and spot-removing cosmetic.

10. The use of the white tomato extract liposome lyophilized powder of claim 9, wherein the whitening cosmetic for oral administration comprises the following raw materials, white tomato extract liposome lyophilized powder, L-cysteine, needle-leaved fruit powder, grape seed extract, black pepper extract and kudzu root powder.

Technical Field

The invention relates to the field of plant application, in particular to a preparation method and application of white tomato extract liposome freeze-dried powder.

Background

White tomato (the name of tomato lycopersicum) is an annual or perennial herb of the genus Lycopersicon, the family Solanaceae, which is sweet in taste and slightly cold in nature. Contains multiple vitamins and also contains polyhydrolycopene (lycopene, phytofluene and phytoene) which is not possessed by other tomatoes. The scientific community proves that both the colorless carotene and the lycopene belong to one of the carotenoids.

Scientifically proved that the white tomato carotene has more advantages in biosynthesis than other carotenoids. They are ideal candidates for blocking UVA and UVB while being effective in inhibiting pigment build-up for at least 4 causes; the white tomato carotene controls pigment accumulation by inhibiting melanin synthesis, and brings more beautiful and white healthy skin color; white tomato carotene has advantages over other carotenoids in biosynthesis. They are ideal candidates for blocking UVA and UVB while being effective in inhibiting pigment accumulation for at least 4 causes.

Disclosure of Invention

In view of the above problems, the present invention aims to provide a preparation method and an application of a white tomato extract liposome freeze-dried powder.

In order to achieve the above object, the present invention adopts the following aspects

The white tomato extract liposome freeze-dried powder comprises a white tomato extract and a liposome membrane layer, wherein the white tomato extract is wrapped inside the liposome membrane layer, and the liposome membrane layer comprises hydrogenated saturated phospholipid and cholesterol as raw materials.

Preferably, the liposome membrane is also wrapped with a vitamin layer.

Preferably, the vitamin layer is composed of hydrophilic vitamins or lipophilic vitamins.

Preferably, the hydrophilic vitamin comprises VB₁₂、VC、VB₃And VB₅(ii) a The lipophilic vitamins include VF and VE.

A preparation method of the white tomato extract liposome freeze-dried powder comprises the following steps:

s1, selecting materials, selecting full white tomatoes without damage on surfaces, cleaning and airing for later use;

s2, preparing a white tomato extract, and preparing a nano white tomato extract by adopting low-temperature crushing and supercritical fluid extraction;

s3, preparing liposome, dissolving the liposome raw material in deionized water to prepare liposome aqueous solution;

s4, preparing a white tomato extract liposome, and wrapping the white tomato extract into the liposome by using a film-ultrasonic dispersion method to prepare the nano white tomato extract liposome;

s5, freeze drying, namely freeze drying the white tomato extract liposome to obtain the white tomato extract liposome freeze-dried powder.

Preferably, in S2, the white tomato extract is prepared, comprising the steps of:

a1 slicing white tomato, wherein the thickness of each slice is not more than 0.2 cm;

a2 pulverizing at low temperature, pulverizing white tomato slices with a low-speed pulverizer at a temperature not higher than 12 deg.C to obtain fine particles with a particle size of 80-120 meshes;

a3 constant temperature extraction, mixing white tomato particles and a solvent to prepare a suspension, wherein the mass ratio of the white tomato particles to the solvent is 1: 6-12; atomizing the suspension by using an atomizer, spraying out, naturally settling liquid mist to recover into suspension, collecting the suspension, and keeping the temperature at 4-10 ℃;

a4, filtering and separating, namely filtering and separating the suspension obtained in the step A2 to obtain clear liquid and residues;

a5 secondary leaching, mixing the residue and solvent to obtain suspension, wherein the mass ratio of Acanthopanax trifoliatus particles to solvent is 1: 4-8; atomizing the suspension by using an atomizer, spraying out, naturally settling liquid mist to recover into suspension, collecting the suspension, and keeping the temperature at 4-10 ℃;

a6, secondary filtration, namely filtering the suspension obtained in the step A4 to obtain clear liquid and waste residue;

a7 solvent separating, mixing the clear liquids obtained in step A3 and step A5, heating, and separating the solvent to obtain white tomato extract.

Preferably, in step a2, the temperature formula is T-10-5 sina, where T is the current temperature and a is the milling time in minutes.

Preferably, in the step A3, the suspension is treated by ultrasonic for 1-3h, the ultrasonic frequency is 200 KHz and the ultrasonic intensity is 3-8W/cm²。

Preferably, in steps A3 and A5, a constant temperature environment is maintained during the extraction process, and the temperature is 6-8 ℃.

Preferably, in step a4, during filtration, multiple filtration membranes are provided, and the membrane pores decrease from top to bottom.

An application of white tomato extract liposome lyophilized powder in cosmetics comprises applying the white tomato extract liposome lyophilized powder as whitening agent.

Preferably, the cosmetics include whitening cosmetics, sun-screening cosmetics, anti-aging cosmetics, and spot-removing concealer cosmetics.

Preferably, the whitening cosmetic is orally taken and comprises the following raw materials of white tomato extract liposome freeze-dried powder, L-cysteine, needle-leaved fruit powder, grape seed extract, black pepper extract and kudzu root powder.

The invention has the beneficial effects that:

the white tomato extract has the effects of resisting oxidation, preventing aging and removing melanin, enables the skin to be white and bright and red, can inhibit the generation of melanin, promotes the metabolism of the skin, accelerates spot fading, evens the skin color, enables the skin to be white and transparent, and is suitable for people with dark skin color, acne and acne marks.

The white tomato extract can reduce melanin generation, whiten skin, prevent deoxyribonucleic acid from being damaged, prevent collagen from being reduced in skin, double whitening effect due to the L-cysteine, effectively exert antioxidant and anti-inflammatory effects, and protect skin from being damaged by ultraviolet rays (UVA and UVB).

The white tomato extract is wrapped by the liposome, so that targeting is realized, damaged cells can be accurately found for repairing, required nutritional ingredients are loaded in the lipid spheres to promote subcutaneous cell absorption, and the whitening effect is strongly transmitted to the dermis of the skin.

Detailed Description

In order to make those skilled in the art better understand the technical solutions in the present application, the following will clearly and completely describe the technical solutions in the present application with reference to the embodiments, and it is obvious that the described embodiments are only a part of the embodiments of the present application, and all other embodiments obtained by a person of ordinary skill in the art without making creative efforts based on the embodiments in the present application shall fall within the protection scope of the present application.