阅读说明：本技术 一株裂解性、高效价副溶血弧菌噬菌体rdp-vp-21010及其应用 (Lytic and high-titer vibrio parahaemolyticus phage RDP-VP-21010 and application thereof ) 是由 杜新永 崔耐 王晓铃 李先胜 马如霞 于 2021-08-05 设计创作，主要内容包括：本发明公开了一株裂解性、高效价副溶血弧菌噬菌体RDP-VP-21010及其应用,其宿主为副溶血弧菌,透过电镜观察,该噬菌体头部呈正六边形,头部直径约为56nm,尾部长度为103nm,为长尾科噬菌体,基因组全长为12558bp,保藏编号为CGMCC No.22481。副溶血弧菌噬菌体RDP-VP-21010对水体中副溶血弧菌具有强裂解作用,具有较高的温度耐受性和较宽的酸碱耐受范围,可以高效治疗该病原菌在水产养殖中所引起的副溶血弧菌疾病,以及可以作为消毒剂,为工业化、规模化、程序化生产噬菌体及治疗副溶血弧菌引起的水产养殖细菌病提供了噬菌体来源。(The invention discloses a lytic and high-titer vibrio parahaemolyticus phage RDP-VP-21010 and application thereof, wherein a host is vibrio parahaemolyticus, the head of the phage is in a regular hexagon shape, the diameter of the head is about 56nm, the length of the tail is 103nm, the phage is a long-tail phage, the total length of a genome is 12558bp, and the preservation number is CGMCC No. 22481. The vibrio parahaemolyticus phage RDP-VP-21010 has strong cracking effect on vibrio parahaemolyticus in water, has high temperature tolerance and wide acid-base tolerance range, can efficiently treat vibrio parahaemolyticus diseases caused by the pathogenic bacteria in aquaculture, can be used as a disinfectant, and provides a phage source for industrialized, large-scale and programmed production of phage and treatment of aquaculture bacterial diseases caused by vibrio parahaemolyticus.)

1. A Vibrio parahaemolyticus phage is characterized in that the phage is Vibrio parahaemolyticus phage (Vibrio parahaemolyticus phage) RDP-VP-21010, and the preservation number is CGMCC No. 22481.

2. The Vibrio parahaemolyticus phage of claim 1, wherein the Vibrio parahaemolyticus phage (Vibrio parahaemolyticus phase) RDP-VP-21010 has a regular hexagonal head with a diameter of about 56nm and a tail length of 103nm, and is a long tail phage.

3. The Vibrio parahaemolyticus phage of claim 1, wherein the Vibrio parahaemolyticus phage (Vibrio parahaemolyticus phase) RDP-VP-21010 has an optimum temperature of 37 ℃ and a titer of up to 1.7 x 10^12 pfu/mL.

4. The Vibrio parahaemolyticus phage of claim 1, wherein the Vibrio parahaemolyticus phage (Vibrio parahaemolyticus phase) RDP-VP-21010 has a strong lytic effect on Vibrio parahaemolyticus in an aqueous body.

5. Use of the Vibrio parahaemolyticus bacteriophage RDP-VP-21010 according to any one of claims 1 to 4 in the preparation of a medicament for the prevention and or treatment of a disease caused by Vibrio parahaemolyticus.

6. Use of the Vibrio parahaemolyticus bacteriophage RDP-VP-21010 according to any one of claims 1 to 4 for the preparation of a feed for the prevention and or treatment of diseases caused by Vibrio parahaemolyticus.

7. A bactericidal composition for controlling Vibrio parahaemolyticus comprising an effective amount of the Vibrio parahaemolyticus phage (Vibrio parahaemolyticus phase) RDP-VP-21010 of claim 1.

8. Use of a Vibrio parahaemolyticus phage according to any one of claims 1 to 4, wherein the Vibrio parahaemolyticus phage (Vibrio parahaemolyticus phase) RDP-VP-21010 is used to provide a potential therapeutic agent or nutraceutical for bacterial infection caused by Vibrio parahaemolyticus.

9. An additive for preventing and/or treating diseases caused by Vibrio parahaemolyticus, which comprises as an active ingredient Vibrio parahaemolyticus phage (Vibrio parahaemolyticus phase) RDP-VP-21010 as set forth in claim 1.

10. A cleaning or disinfecting agent for an aquaculture environment comprising Vibrio parahaemolyticus phage (Vibrio parahaemolyticus phase) RDP-VP-21010 of claim 1.

Technical Field

The invention relates to the technical field of biology, in particular to a lytic and high-titer vibrio parahaemolyticus bacteriophage and application thereof in vibrio parahaemolyticus infection in aquaculture.

Background

Vibrio parahaemolyticus, also known as pathogenic halophilic bacteria, is a marine pathogenic bacterium and is a zoonosis pathogen. Isolated in 1950 from an explosive food poisoning in japan. The vibrio parahaemolyticus is extremely wide in distribution and mainly distributed in seawater and aquatic products, and according to statistics, the detection rate of the vibrio parahaemolyticus in coastal seawater in east China is 47.5% -66.5%, the average bacteria carrying rate of marine fishes and shrimps is 45.6% -48.7%, and the detection rate can reach more than 90% in summer. The existence of Vibrio parahaemolyticus is found in livestock and poultry meat, salted vegetables, salted eggs, freshwater fish, and the like, in addition to marine products.

In recent years, the aquaculture industry in China is rapidly developed. With the increasing expansion of the mariculture scale and the continuous popularization of intensive culture modes, the frequent occurrence of fish and shrimp diseases poses serious threats to the healthy development of the large-scale culture of aquatic products. The pathogenic factors of aquatic animals mainly include bacteria, viruses, parasites and the like, wherein bacterial diseases are diseases with serious damage degree to the aquatic animals. At present, the main method for preventing and controlling vibrio parahemolyticus is to use antibiotics, although the antibiotics are convenient to use, have broad-spectrum bacteriostasis and quick response, the abuse of the antibiotics also causes the serious drug resistance of bacteria, and the effect in preventing and treating diseases caused by the vibrio parahemolyticus is increasingly reduced, so that the development of novel vibrio parahemolyticus resistant drugs is urgently needed.

The bacteriophage is a bacterial virus, has higher abundance in nature and strong host specificity, and cannot cause damage to the micro-ecological environment. After the phage infects host bacteria, new phage can be released during cracking, and a small amount of phage can play a role in preventing and treating. The action target of the vibrio parahaemolyticus phage is on the cell wall of prokaryotic microorganisms, and corresponding action sites do not exist on animal and plant cells, so that the use safety of the phage is improved. And the phage and the pathogenic bacteria can evolve together to prevent the occurrence of bacterial drug resistance. Therefore, the utilization of the phage to solve the biological control approach with wide prospect for bacteria.

The invention provides a safe and reliable lytic vibrio parahaemolyticus phage for treating vibrio parahaemolyticus in response to national order of comprehensive banning of resistance, solves the problems of bacterial drug resistance and antibiotic abuse, and lays a foundation for developing antibiotic substitutes for treating vibrio parahaemolyticus by expanding a phage stock library.

Disclosure of Invention

The invention aims to provide a specific lytic vibrio parahaemolyticus bacteriophage RDP-VP-21010 to replace antibiotics in preventing and treating aquaculture and solve the problems that aquatic animals are not treated timely due to acute disease, drug taking is ineffective due to drug resistance of pathogenic bacteria and the like.

In order to achieve the purpose, the invention adopts the following technical scheme: a lytic vibrio parahaemolyticus phage RDP-VP-21010 is characterized in that the head of the phage is in a regular hexagon shape, the diameter of the head is about 56nm, the length of the tail is 103nm, and the preservation number is CGMCC No. 22481.

The invention separates a vibrio parahaemolyticus phage from culture sludge by using a double-layer agar plate method, and the whole length of the genome of the vibrio parahaemolyticus phage is 50642bpThe phage has high titer (2.2 × 10) when it acts for 6 hr at pH 5-10¹⁰Above pfu/mL, there is a clear alkalophilicity. At pH 12, the titer can reach 10⁶pfu/mL, at pH4, the titer can still reach 10⁷pfu/mL, the phage was found to have a wide range of acid-base tolerance. The optimum temperature of the phage is 37 ℃, and the titer can reach 1.7 multiplied by 10¹²pfu/mL, the bacteriophage can be preserved for a long time at-80 deg.C, and the titer is maintained stable. The bacteriophage can specifically crack pathogenic vibrio parahaemolyticus BV-21002 separated from diseased shrimp foci obtained from Qingdao ink farmer in the laboratory, and the optimal infection complex number is 1: 1. The phage is named as vibrio parahaemolyticus phage RDP-VP-21010, and the phage is preserved in China general microbiological culture Collection center in 2021, 06 and 03 months, with the preservation addresses as follows: no. 3 Xilu No. 1 Beijing, Chaoyang, and the preservation number is CGMCC No. 22481.

The invention also provides application of the vibrio parahaemolyticus phage RDP-VP-21010 in preparation of a vibrio parahaemolyticus resistant preparation.

The invention also provides application of the vibrio parahaemolyticus phage RDP-VP-21010 in preparation of anti-vibrio parahaemolyticus BV-21002 drugs.

Another purpose of the invention is to provide an application of the vibrio parahaemolyticus bacteriophage RDP-VP-21010 in preparing medicines for preventing and or treating diseases caused by vibrio parahaemolyticus.

Another purpose of the invention is to provide an application of the vibrio parahaemolyticus bacteriophage RDP-VP-21010 in preparing feed for preventing and/or treating diseases caused by vibrio parahaemolyticus.

Another purpose of the invention is to provide a bactericidal composition for preventing and treating Vibrio parahaemolyticus infection, which comprises an effective amount of Vibrio parahaemolyticus RDP-VP-21010.

Another object of the present invention is to provide the use of a Vibrio parahaemolyticus bacteriophage which provides a potential therapeutic drug or health product by using the Vibrio parahaemolyticus bacteriophage RDP-VP-21010 as a bacterial infection caused by Vibrio parahaemolyticus.

Another object of the present invention is to provide an additive for preventing and/or treating diseases caused by Vibrio parahaemolyticus, which is achieved by including Vibrio parahaemolyticus bacteriophage RDP-VP-21010 as an active ingredient.

The invention also provides a cleaning agent or a disinfectant, which comprises vibrio parahaemolyticus phage RDP-VP-21010.

The invention has the beneficial effects that:

(1) the invention discovers and separates a pathogenic vibrio parahaemolyticus BV-21002, and separates a vibrio parahaemolyticus bacteriophage RDP-VP-21010 by taking the pathogenic vibrio parahaemolyticus BV-21002 as a host, the bacteriophage RDP-VP-21010 has strong cracking effect on the pathogenic vibrio parahaemolyticus BV-21002 in an aquaculture environment, and provides a bacteriophage source for the industrial production of the bacteriophage for preventing and treating the pathogenic vibrio parahaemolyticus BV-21002 in the aquaculture environment;

(2) the vibrio parahaemolyticus phage RDP-VP-21010 provided by the invention is a virulent phage test phage separated from nature and does not contain virulence genes or undesirable genes; the invention does not carry out any genetic modification on the test phage;

(3) the vibrio parahaemolyticus phage RDP-VP-21010 provided by the invention has an obvious lysis effect within 8 hours after the vibrio parahaemolyticus phage RDP-VP-21010 is added into a water body, has strong lysis activity and shows a good application prospect.

(4) The vibrio parahaemolyticus phage RDP-VP-21010 provided by the invention acts for 6 hours at pH 5-10, the titer is high and can be 2.2 x 10¹⁰Above pfu/mL, there is a clear alkalophilicity. At pH 12, the titer can reach 10⁶pfu/mL, at pH4, the titer can still reach 10⁷pfu/mL, the acid-base tolerance range of the phage is wider, higher temperature tolerance and wider acid-base tolerance range are shown, the titer is higher, and the safe and effective effects are realized;

(5) the vibrio parahaemolyticus phage RDP-VP-21010 provided by the invention has a good application prospect in prevention and control of pathogenic vibrio parahaemolyticus of aquatic animals.

(6) The vibrio parahaemolyticus phage RDP-VP-21010 provided by the invention can be used as an effective component of various products applied to environmental disinfection.

(7) The vibrio parahaemolyticus phage RDP-VP-21010 can be used as an effective component of various products applied to environmental disinfection, for example, the vibrio parahaemolyticus phage RDP-VP-21010 can be used for disinfecting and decontaminating surfaces of water distribution systems, medical facilities, aquaculture facilities, public and private facilities or other environments in the forms of liquid soaking, spraying, combined use with aqueous carriers and the like, and can effectively control the growth and activity of target bacteria. The liquid soaking, spraying forms include but are not limited to detergents, disinfectants, detergents, etc.; the aqueous carrier includes but is not limited to phosphate buffer, LB medium, chloride free water, etc.

(6) The vibrio parahaemolyticus phage RDP-VP-21010 can effectively kill vibrio parahaemolyticus on the surface of marine products and prevent and treat the pollution of the vibrio parahaemolyticus in the preservation process of the marine products, and can be used as an effective component of various products for protecting marine products. The present invention includes, but is not limited to, the prevention of food spoilage caused by infection with Vibrio parahaemolyticus in the form of liquid immersion, spraying, use in combination with synthetic ingredients, and the like, and is particularly suitable for cooked or non-sterilizable foods. The liquid soaking and spraying forms comprise but are not limited to food degerming agents, food disinfectants, food preservatives and the like; synthetic components of the present invention include, but are not limited to, benzoic acid, sodium benzoate, sorbic acid, potassium sorbate, calcium propionate, and the like.

(7) The vibrio parahaemolyticus phage RDP-VP-21010 of the invention can be used for preparing compositions, reagents or kits; the method can be applied to the rapid detection of the vibrio parahaemolyticus, and comprises the steps of detecting the vibrio parahaemolyticus in a target sample in the forms of test paper, a kit and the like, or screening target pathogenic bacteria in a clinical sample, so that the detection sensitivity can be effectively ensured;

(8) the vibrio parahaemolyticus phage RDP-VP-21010 can be used alone or in a mixture, and can be used as a biological bactericide, a feed additive, or a therapeutic drug, a health product and a medical apparatus for bacterial infection caused by vibrio parahaemolyticus; the vibrio parahaemolyticus phage RDP-VP-21010 can be applied to the treatment or prevention of infectious diseases caused by vibrio parahaemolyticus;

(9) the vibrio parahaemolyticus phage RDP-VP-21010 can be applied to industrial production, can be specifically amplified by host bacteria, can be highly purified by applying a standard virus purification method, and has a very good popularization prospect. According to the description of the invention and the common knowledge in the field, the technical personnel can prepare the vibrio parahemolyticus phage RDP-VP-21010 or the composition thereof into various products applied to the aspects of medical treatment, detection, disinfection, food protection and the like for industrial application. The product form can include, but is not limited to, the carrier carrying, the concentrated injection or the medicament soaking and the like, and the product is applied to the controlled host body surface, the oral part, the rectum, the inner pleura and other parts; as one embodiment, the carrier-borne form includes, but is not limited to, oral aqueous carriers, oral anhydrous carriers, cream formulations, and the like; concentrated injection forms include, but are not limited to, vaccine injections, pleural cavity injections, transvenous injections, etc.; dosage soaking forms include, but are not limited to, aerosols, rinses, and the like.

Drawings

The above and other features, properties and advantages of the present invention will become more apparent from the following description of the embodiments with reference to the accompanying drawings:

FIG. 1 is an electron micrograph of bacteriophage RDP-VP-21010 according to the present invention;

FIG. 2 is a schematic acid-base stability of bacteriophage RDP-VP-21010 of the present invention;

FIG. 3 is a graph showing the lysis of the bacteriophage RDP-VP-21010 of the present invention against the host bacterium BV-21002;

FIG. 4 is a schematic thermal stability diagram of bacteriophage RDP-VP-21010 of the present invention;

FIG. 5 is a schematic representation of the genetic stability of bacteriophage RDP-VP-21010 of the present invention;

FIG. 6 is a schematic diagram of RTD experiment of the bacteriophage RDP-VP-21010D of the present invention

Detailed Description

The invention is further illustrated with reference to the following figures and examples. The following examples will assist those skilled in the art in further understanding the invention, but are not intended to limit the invention in any way. It should be noted that variations and modifications can be made by persons skilled in the art without departing from the spirit of the invention. All falling within the scope of the present invention.

The invention separates vibrio parahaemolyticus BV-21002 from a diseased shrimp focus of a Qingdao, i.e. a Munich farm, determines pathogenicity and drug resistance of the BV-21002 through experiments, separates a lytic vibrio parahaemolyticus phage from culture sludge by taking the BV-21002 as a host, and determines the genotype of RDP-VP-21010 through a whole genome sequence.

Example 1 isolation and identification of pathogenic bacteria and phages

(1) Isolation and identification of pathogenic vibrio parahaemolyticus BV-21002

Sampling from gills of the ill prawns, coating the ill prawns on a TCBS culture medium by adopting an aseptic operation method, culturing at 37 ℃ for 18-24 hours, and then presenting round green colonies with neat edges on a TCBS agar plate, wherein the diameter of the round green colonies is about 2-3 mm; then selecting a typical colony to streak on a Vibrio coimajiaensis color development culture medium, culturing for 18-24h at 37 ℃, then presenting a purple colony on the Vibrio coimajiaensis color development culture medium, selecting the typical colony to continuously streak and purify for 3 times, then selecting a single colony to inoculate in 5mL of 2216E liquid culture medium, culturing for 6h at 37 ℃ and 200rpm in a shaking way, and obtaining a uniform and turbid bacterial suspension. Then identified as vibrio parahaemolyticus by 16sRNA molecular identification, one of the vibrio parahaemolyticus is named BV-21002 and stored in a refrigerator at minus 80 ℃.

(2) Phage isolation and identification

The sludge sample for the test is collected from the water bottom sludge of Qingdao, i.e. inkfarmer in 2021 years and is used as a sample for phage separation.

Sample treatment: soaking a proper amount of sludge in 20mL of 2216E liquid, centrifuging at 10000rpm for 10 minutes, removing larger impurities and most bacteria, and filtering and sterilizing by using a 0.22-micron microporous filter membrane.

Preparing a mixed bacterial suspension: 2mL of the filtrate and 1mL of the host cell suspension were added together to 27mL of 2216E liquid medium after autoclaving, and then placed in an incubator at 37 ℃ for overnight culture. After culturing, 3mL of the solution is taken, centrifuged at 10000rpm for 5 minutes, filtered and sterilized by a 0.22 mu m microporous filter, and the filtrate is collected for later use, namely the stock solution which is supposed to contain the phage.

Phage separation: separating phage by double plate method, mixing the mixed bacterial suspension filtrate 0.1mL and secondary hemolytic vibrio suspension 0.1mL, adding into 2216E agar heated to 45-54 deg.C 3mL 4%, spreading double plates, culturing in 37 deg.C incubator for 6-8h, observing result, if there is phage, there is transparent plaque on the plate. Soaking plaque in 1mL of 3% saline for 10min, adding 0.1mL of leachate and 0.1mL of bacterial suspension into 3mL of 2216E agar heated to 45-54 ℃, spreading a double plate, culturing in a 37 ℃ incubator for 4-6h for purification, and purifying for 2-3 times according to the steps until the plaque is uniform in size, thereby obtaining a phage, and the phage is named as RDP-VP-21010. The plaque formed by RDP-VP-21010 on the double plate has a diameter of 4mm-5mm, a round shape, transparency and a halo.

(3) Measurement of cell titer

Laying a double-layer plate on the purified phage, taking a single plaque, adding the plaque into 2216E liquid culture medium, simultaneously adding host bacterial suspension, after the phage is proliferated, centrifuging the proliferation liquid at 10000rpm for 5 minutes, filtering by using a 0.22-micrometer microporous filter, performing 10-fold gradient dilution on the filtrate to 10-8 times, taking 0.1mL of phage diluent and 0.1mL of host bacterial suspension with the last 3 dilutions, laying the double-layer plate, and determining the titer of the phage. Phage titer (pfu/mL) ═ plaque number dilution factor ÷ 0.1. The result shows that the plaque has consistent shape, size and definition and has typical lytic phage characteristics; the titer of the phage can reach more than 10^12 pfu/mL.

(4) Preservation of phages

Phage multiplication fluid was mixed with 60% glycerol according to 1:1, and storing in liquid nitrogen.

EXAMPLE 2 Electron microscopy of phages

Dropping 20 μ L of liquid containing crude phage particles on a copper net, naturally precipitating for 15min, sucking off excessive liquid from the side by using filter paper, adding a drop of 2% phosphotungstic acid (PTA) on the copper net to dye the phage for 10min, sucking off the staining solution from the side by using filter paper, and observing the form of the phage by using an electron microscope after a sample is dried.

As can be seen from FIG. 1, this phage RDP-VP-21010 is a Long-tailed phage with a regular hexagonal head diameter of about 56nm and a tail length of 103 nm.

Example 3 phage genome sequencing

After enrichment culture of a single phage, centrifuging for 15 minutes at 8000g under the condition of 4 ℃, adding 10% PEG8000 and 0.5M NaCl, standing overnight, adding equal amount of chloroform, mixing uniformly, standing for layering, centrifuging for 10 minutes at 5000g, removing a chloroform layer and a PEG layer, adding restriction endonuclease for digestion treatment, suspending the phage under the condition of gradient density cesium chloride, dialyzing for 3 times by TM buffer solution at the later stage, and sending the phage to a Huada gene biological sequencing company for whole genome sequencing after 30 minutes each time. The results are shown in the sequence listing.

Example 4: determination of phage pH stability

Adjusting pH of normal saline with dilute hydrochloric acid and dilute NaOH solution to obtain buffer solution with pH of 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, and diluting phage with prepared buffer solution to 1 × 10¹⁰pfu/mL, putting the diluted solution in a water bath at 37 ℃ for 1h, diluting with 10 times of normal saline, and then spreading a double-plate; counting after inverted culture at 37 ℃ for 4-6 h.

As can be seen from FIG. 2, after 1h of incubation under different pH conditions, the phage RDP-VP-21010 can maintain better activity in the pH range of 4.0-11.0, the phage titer does not change significantly, and the phage titer is highest at pH 8.0, reaching 5X 10⁹pfu/ml. The titer of the phage decreased significantly with increasing or decreasing pH. The results show that the bacteriophage RDP-VP-21010 can resist weak acid and strong alkali conditions and has good acid-base tolerance.

Example 5: determination of optimal multiplicity of infection

The phage multiplication solution and the host were added to 2216E in a ratio of multiplicity of infection of 10:1, 1:10, 1:100, 1:1000, 1:10000, and the total volume of the culture system was kept the same. After shaking culture at 37 ℃ and 200rpm for 8h, centrifuging at 12000r/min at normal temperature for 5min, and spreading the supernatant on a double-plate to determine the titer. The results are shown in Table 1.

TABLE 1

As can be seen from the data in Table 1, when the infection complex number is 1:1, the proliferation medium is relatively clear and the titer is the highest after 8 hours of culture, indicating that the optimal infection complex number of the Vibrio parahaemolyticus phage RDP-VP-21010 of the present invention is 1: 1.

Example 6: phage versus host lysis curve

The host bacterial suspension cultured for 6h was inoculated into 100mL of 2216E medium at a ratio of 1:1, and cultured with shaking at 37 ℃ and 200rpm for 2h (OD about 0.45, about 6.0X 10)⁸cfu/mL) of the bacterial suspension was divided into 4 sterilized Erlenmeyer flasks, and 0.2mL (about 4.0X 10) of a phage-proliferating solution was added to 3 of the Erlenmeyer flasks at the optimum multiplicity of infection¹¹pfu/mL, filtered through a 0.22 μm membrane), while taking the average value with no phage added as a control, the results are shown in FIG. 3.

As can be seen from FIG. 3, the Vibrio parahaemolyticus phage RDP-VP-21010 is added into the suspension of the host bacterium BV-21002, after 1h, a certain lysis effect appears, after about 1h, the OD600 value begins to decrease, the effect lasts until 8.5h, after 9h, the mixed bacterium solution begins to become turbid, the OD value gradually increases, and the balance is reached after 12h, but the OD value is still lower than that of the host bacterium. In the early stage, the phage is in the adsorption and infection stage of the host bacteria, only a small part of the phage starts to crack the host, so the amount of the cracked host is far less than the growth amount of the host, the OD600 still keeps a certain rising trend in the first 1h, the number of the cracked host is larger than the proliferation speed of the host along with the exponential increase of the number of the phage, the OD600 value starts to be gradually reduced, and the mixed bacteria liquid is clearest. After the continuous action for 9 hours, the mixed bacteria liquid turns turbid, the original sensitive bacteria are cracked by the bacteriophage, and the flora replaced by the mutant tolerant bacteria is alternated.

Example 7: determination of the thermostability of phages

The phage stock solution is subpackaged into EP tubes, incubated for 30min at 40 ℃, 50 ℃, 60 ℃ and 70 ℃, diluted by 10 times of normal saline, and spread on double plates to determine the titer. The results are shown in FIG. 4.

As can be seen from FIG. 4, the activity of the phage was kept stable at 40 ℃ to 60 ℃ and the titer was 10¹²pfu/mL or more; after the phage is acted for 30min at 70 ℃, the titer can still reach 7.4 x 10^4pfu/mL, which indicates that the phage has certain temperature tolerance.

Example 8: genetic test for stability

The RDP-VP-21010 is continuously passaged for 15 times, the same amount of RDP-VP-21010 and BV-21002 is added into each generation of culture medium, the culture time of each generation is 6h, and then double plates are paved to determine the titer. The results are shown in FIG. 5.

As shown in FIG. 5, the titer of RDP-VP-21010 was measured after continuous passage through 15 generations, and the titer stabilized at 10¹¹And pfu/mL or more indicates that the phage has better genetic stability and is suitable for industrial production.

Example 9: cracking rate test

Under aseptic conditions, 1mL of sample and 1mL of host bacterium solution (1X 10)⁶CFU/mL), incubating for 15 minutes at 37 ℃, mixing uniformly, and diluting to 10 with physiological saline^-1-10^-3Each gradient was plated at 100. mu.L on 2216E agar plates and incubated at 37 ℃ for 24 hours, with each gradient repeated twice. Simultaneously, 1mL of 3% saline and 1mL of host bacterium solution (1X 10)⁶CFU/mL) as a blank, repeat the above steps. Plates with 30-300 colonies were picked for enumeration. The test was repeated 3 times and the average was taken. The phage lysis rate was (1-number of treated colonies/number of control colonies) × 100%.

Through calculation, the cracking rate of the RDP-VP-21010 reaches 93.1%, and the RDP-VP-21010 has a good cracking effect on a host and is suitable for being used in a culture process.

Example 10: RTD experiment

The plates were divided into zones, a drop of host bacteria (about 10. mu.L) was added to the center of each zone, air dried, a drop of phage was added at different dilutions to the spot of host bacteria, air dried, and incubated overnight at 37 ℃. The result is shown in FIG. 6, where the host bacteria cannot grow continuously, and is called CL concentration (CL concentration is the concentration of host bacteria in the environment that can be completely lysed and can be used as a dilution scheme for guiding the production practice).

As can be seen from FIG. 6, RDP-VP-21010 is diluted 10⁵After doubling, the bacillus subtilis still has better lysis effect on host bacteria.

Example 11: method for large-scale production of bacteriophage

The process flow is simple, reliable and effective, and is favorable for industrial production.

The concrete steps

1. Seed preparation

(1) Preparing a host seed: under aseptic condition, selecting single colony, streaking on 2216E agar medium, culturing at 37 deg.C overnight,

then, the cultured single colony is picked up and put into 5mL 2216E liquid culture medium, shake culture is carried out for 6h at 37 ℃ and 180rpm, then the single colony is inoculated into the 2216E liquid culture medium by the inoculation amount of 1%, and shake culture is carried out for 6h at 37 ℃ and 180 rpm. The prepared seed liquid is stored in a refrigerator at 4 ℃ for later use.

(2) Preparing a phage seed: diluting the preserved phage seeds properly, placing 100 μ L and 100 μ L host bacteria in the upper layer culture medium, pouring the double-layer plate, culturing at 37 deg.C for 4-6h, scraping with sterile coating rod, centrifuging at 10000rpm for 5min, sterilizing with 0.22 μm filter membrane, and storing the collected filtrate in 4 deg.C refrigerator.

The phage and the host are respectively inoculated in 2216E liquid culture medium in the proportion of 1 percent and 5 percent, and cultured for 6-8h at 37 ℃ and 180rpm until the proliferation liquid is clear and a large amount of floccules appear.

2. And (3) phage multiplication: inoculating 2% of host bacteria in a seeding tank, culturing for 2-3h, then inoculating 0.6-0.8 OD600, then inoculating 1% of the inoculum size to phage seeds, culturing for 6-8h, until the dissolved oxygen is reversely increased and the pH value tends to be stable.

Inoculating 2% of host bacteria in a seeding tank, culturing for 4-5h until dissolved oxygen begins to slowly decrease.

Inoculating the prepared host bacteria into a large fermentation tank with the inoculation amount of 5%, culturing for 2-3h until dissolved oxygen begins to slowly decrease, then inoculating phage seeds with the inoculation amount of 5%, increasing the ventilation amount to 1vvm, increasing the rotation speed to 150rpm, culturing for 8-10h until the dissolved oxygen reversely increases and the pH value increases, and finishing proliferation.

Note: fermentation tank parameters: the seeding tank has 200-300rpm, 37 deg.c and ventilation amount of 0.6-1 vvm. Fermentation tank: 120-150pm, 37 ℃ and the ventilation volume is 0.6-1 vvm.

The components of the culture medium: 0.5% of yeast extract powder, 1% of peptone, 0.1% of beef extract, 0.5% of glycerol, 0.5% of sodium chloride, 0.1% of monopotassium phosphate, 0.1% of dipotassium phosphate, 20ppm of calcium chloride and 20ppm of magnesium chloride.

3. Phage post-treatment process

(1) And (3) filtering: and (3) respectively adopting 800nm and 500nm ceramic membranes for filtration to remove cell fragments and host bacteria which are not cracked, wherein the filtration pressure is less than 0.4mpa, and the damage of shearing force to the bacteriophage is reduced.

(2) Concentration: hollow fiber membranes are used for concentration to improve the titer of the phage.

(3) A stabilizer: adding 5% of glycerol, 3% of trehalose, 3% of polysorbate, 3% of sodium dihydrogen phosphate and 2% of sodium phosphate, and stirring until the mixture is completely dissolved.

(4) And (3) degerming: three-stage filtration (glass fiber 0.45 μm, glass fiber 0.2 μm, polyethersulfone 0.22 μm) was used for sterilization.

(5) The filter-sterilized phage liquid was stored at 4 ℃.

It should be noted that the specific embodiments are merely representative examples of the present invention, and it is obvious that the technical solution of the present invention is not limited to the above examples, and many variations are possible. Those skilled in the art, having the benefit of this disclosure and the benefit of this written description, will appreciate that other embodiments can be devised which do not depart from the specific details disclosed herein.

Sequence listing

<110> Ray union (Qingdao) bioengineering Co., Ltd

<120> lytic and high-titer vibrio parahaemolyticus phage RDP-VP-21010 and application thereof

<160> 1

<170> SIPOSequenceListing 1.0

<210> 1

<211> 87990

<212> DNA

<213> 2 Ambystoma laterale x Ambystoma jeffersonianum

<400> 1

aaaacttcaa cgagtttaac ttgttccgta aaccagatgc ttgggatcgt gaacctaaca 60

gtgcttacaa gcttcttatg gagagtgttc agaataataa acaccttatt gatgaacgtg 120

tccaagaagt tattaaaggt ttacaaccac gagaagattg gaacattgat cttgattgtc 180

gtatcggtga tttaatctta actaaattgt ttaaggatga atagttatga caacacaact 240

tacttatcat gttggtatct tatctccaag agctgaggta gttgtttaca acagacagaa 300

taaagttgag tataagatcc tatcagatta tgcttctgaa agtaatattc gttctcatgt 360

aggtaactat gggtcagggc aatacgaccc ttactacaac ggcttctatg gtaatcgtaa 420

aacagctatt actcgtttag aagatgaaat taagagaact gagagttatt tagaagcact 480

taaattctct ttgaaagtta ctaagaaaaa ggtatgggag gaaataaaat gatcacacca 540

ataatcaatg gcgatggtga agtttgcatc gatgtaatag atgtgtttga taatacaagt 600

gaccaattca aacttgagct tgtagaacgt ctatcttgtg aagatgtaat catcaaacat 660

gttattgacc aagtattaaa gggctgcaca gataatgggt atagtggttc agaagtgatt 720

gacaatttac gtttaggtag tgaactacaa caagcccgag aacgtattcg ccaacaaggt 780

aacaacttac ttgttaaaga aatcaatcgt ctacgtggag tattggaaaa tcgttgtaaa 840

tatcaagata aaggttggaa tgaatatcac aagctctata atcaagttaa tcgttgttca 900

gacatttact tgtaaataaa tcttcaagca ggggttgtgc tgacacataa tctctgctat 960

atttatatcc taaacgtaag gataaaggag aaacatcatg aatcttgata tggtaaactg 1020

cattattcga tttgataaaa ctattgacga agataataat cctgtttgga atgaaactta 1080

tttgaaagag tttgtattct atgggagtgg tggtgctagt gatgcacaaa aaggtattga 1140

taaagctaaa gaattaaact ctcaacacaa aacgcaatct cgttattaca tggctgttcg 1200

tattgatata tctaaagctg tgaaacttgg attgtttgat gagggagata atctatgagt 1260

tatgatgttg ttaaaggttg tgagagctgg gattaccatg tagtggatat tttatcagga 1320

aatgaacctg tagctggttt ctttaatcaa gatatggcaa attcttattg tgaggatttg 1380

aatagacata gaagtttatc atgttgtaaa atcagtatca gtacttctgg ttggcatcac 1440

gaccatgatt gcaaaggtgt ttatgaaaaa cctgtaacct atgataagta tcgtgtttat 1500

ctttgtgggg ataatcctac agttttcaaa caaatctcac ataataagta tgaatgtaca 1560

catggtggtt ggtcattcga agaaaggtta gatagacctt ctaatgaaat ctacttacca 1620

tataaaaaga aacctttagt ggtagctgat tctatgattg agtttaatag tatacaagag 1680

gttattgaag cagggtataa gggatatttg cgatgaatta tgatccagat acaatcttta 1740

acgggaaagc tgtgttacaa gtaaatactt ctgacccttc aaacaaggtt gtattatttg 1800

taaatgataa acattcttat atcagagaac acttcttgtg ggaacaaaag tatagtaaga 1860

atggtgaact tctaccacaa gcaaaattta ttttattaaa agacattact actttacatt 1920

tgaaaaactt gtgctattct agtgttaatt ggaaagataa gaagttgaat aaagtttttg 1980

ttgatgagtg gaattggaga attgataatg aataaagaat ttaacattgg tgaaaaactt 2040

tcaccagaag atattgaagt tggtatgatt gtcgatgtct tgacggggag ttgtggttac 2100

aaggagtgta cgatactgtt attgaaacat gattttgtaa gcttcgaaac tcctgatttt 2160

gaaggagctg ttttcaacta tgagaggttt aggaataatc gatcaataaa gttccttttt 2220

agaggttaca gtcaaaactt tattgtaagc ttcacagaca atacttctta cttatctgaa 2280

caatatatcg cagaagcaat taaaaccttg tcacgggctt cacaggagct ttctacacat 2340

gttacagttg atggaaaagg tgttgtgacg gtttacagtg attatcacga agaagattta 2400

gtgtttgaca ctgtagagaa gttaatgcag tatattgagt gtgttaaaca gctggataaa 2460

tttaaataga gggtgtgaag aatgagtaag aaatctaaag tatcatattc aataggtaca 2520

ctaaattacc tatcaaacga agctctcgat gagataatca gaggtgataa ttatgataat 2580

gaaacaaaga aactagctac cagagttctg aatgcaagag gtatgtatga ctaaacaaaa 2640

ttatgatcca tcacaagtga cagtagagat taacggtcaa gttttgcagt atgttttctc 2700

tacaacagaa tcactcaaaa cacctgacat ctacagcaca caatcactac aaatcaatag 2760

tctttgtgag tttgctcgca cactaggtta tgttccaaga gatttggata tgatttcact 2820

aggttttgag attgttgata tgcgctcttg gcacacacat tacatctctt ttaagaacat 2880

gtgtaaactt cataacggat tcttacaccg tgttatccat ttatcagatg atgttaaagc 2940

ttacgacttc ttagagttaa aacatattga cattcctaca gataagcttg aattagcttg 3000

gaacaaccga cttgtagaac gtgtaaaact acaacgtgat aagaattcat acggtggtgt 3060

gaaagttcaa agtaataaag ttaagcttac ggataaaggc aaggaatatt gtgcacagtt 3120

tgatcgttac gattttcttt ttcttgaaga ttaaaggtgt gaacatggtt gttcatgatt 3180

tataccactt tcagaagtat tgtaatcttg atgattatta tggatatcgc cgtttgtgtg 3240

ttgacacaat tggttggagg ttgtatagtt tgtcttggaa gtggaaatac ttttggaatt 3300

tattagttta taaagttgcc aaacaagttt agggagatcg actatgaaag aaaaacttta 3360

tacgccagat ttagctggta gtggacaatt tgcaaggtgt gcacattgta agaaactacc 3420

aactccagaa ggacatgatg gttgtcttgg aacactacca gaaaacatta tcatgaacgc 3480

ttgttgtggt cacggagata tagaagtagc ttatattcag ttttgggata gtaagtgtgt 3540

tcgtggtaaa gaagcaatgg aaattatcaa tagtttaaag gaggactgag ggatgaacaa 3600

aaagtttcgt aaagattttc atcgtaattt tccttggtat gattacgcct ttctccttga 3660

agcaatgttg ttatggtcag agaaagcctc agaacgtcat aagaagcatg gaaacttggt 3720

aaggagcgat gatactagta agaggctaaa acttgtctca gagcttctca agcgtcttat 3780

cgctgatgat tatgatgagc cgtgcaaagt tttcaatagt aggaataaaa ctttgtcaaa 3840

aggtaataac tttggtataa attttgatga agatttcaca tacttgaaaa gtgaacattc 3900

tatttctcgt agaaatcaag ataagaaaat gttgtttatg ttattagata aacatttgtt 3960

cgagttttgg gattaaataa ggagaagtat tatgaaagaa gtaatgatga ttttaacaat 4020

ttggattgta ggtttcttga ttggattcaa tatgccaaca ctgtttggat ggtggtaata 4080

tggacaataa actcattatt aaccttctta aaacagttgt gaaagaaagt cagaaagagg 4140

aagtcttata tcgaattcct cgtgctgtcg gagggttcag tgaagtaacc aaaacggttg 4200

ttgatgggga taagttaaaa cttggtgtag aaacaattat tgcaatgttg gaaaatgttg 4260

aggagggtga aggatgatta catgtaaaga agatttgatt aatacatata ttgagaatga 4320

ccaaggtgag ttgagagatt tgtacttagc taagtgtgaa gattttaatc ttaaaacagg 4380

gtcagataga cttctccaag acggtatctt tgaccactac gattctgagt gttttgaggt 4440

gattgacgat ggtaaggttt atttcccaaa ctttaaaaac ttatcacaga attcaagaaa 4500

actaaactta ttagacctaa aaccacgaac taaaactgcg tatgaagaag tatcgattgg 4560

tgatgtggat gaatcaatgc ttgatatcgt tttcatcgat ccgaaaggtg atgtgtcaat 4620

tagaaacgct agtgtctcag agtttatttg ctgccaactt taccgtaagg tagaaaaaga 4680

gattgattgg cgagatgaaa tcagtgatta ctttgttgag tatgaaacag cagatggtag 4740

tggtgagtat agaattgata gtgacgactt cctagatgta tgtcgaaaag tattacgtgc 4800

aacaggagaa ttaggatgaa cttaatattc gagctagatg attggagtgt tgaccatgaa 4860

atcattatta aatttaaaaa taacaagttt gattggattg atcctattgt gacagtgtat 4920

gaagatgatg ggaagctggt tgtagagaat gagagtggta aattttgaaa atggaagaaa 4980

aataatttaa atagattgga atataagagg tattagatgt atgcaagtaa aattaagagt 5040

ttgacagaaa aagaagttga aaatctagag cattataagg tgaattctaa atttattggt 5100

tttgattatg aaaatgcaac agttataaaa acctttggct ccagaaacag atggaatatg 5160

gtatttaaat gttgttgtgg agaatatttt tatgtcgata acggaaagtt gggttatgca 5220

ttatcagcag gtaatttagc taaagtaaat tgtggttgta ttgatgaaaa cgcagagaaa 5280

attaaattag aggaagctaa atctatcttc tataataaac aattacggtc agaaactaat 5340

caacactggt gcacctattg cgaatcttat cgtccagaag ttaaacagaa gagtaagaac 5400

acctttcatt gcagtaattg tgatttctta gcaagtaaag ctatagtttt aggagaaaca 5460

tttgaggaaa ttatggcaag atgggattcg acaacacaca aaccttgtac caagaagtta 5520

ccttatctag aaggaaatag taagaaaggg tacaaagtaa gaggtttcac ttatatatct 5580

tctgactggt atgatgaggt aagtaagtgg ttatggatta aagttaaaga ttacataaaa 5640

acatctttct ctaaggataa cttaagtaga ataggtaaat tacacttata taaaacttca 5700

aaagatagga agtatagagg tatcttttta catagattag ttcttggatt aggtaatgat 5760

gtaagtatgg taggtgacca cttaagtggt tataaattgg ataataggaa tagcaattta 5820

agatatataa ccgcatacca gaacagttta aactcaaaga agagttcaaa aagggcaggg 5880

aatagtaggt ataaaggggt aagtttctac aaagatagat ttgacaaagg ttgtcgttgt 5940

tggaaagcta ctttgtccat ctcaggtaaa tataggacta agtactttta tagagaagta 6000

gaagcagcta agtcttatga tagtatgcta agggaatatt tacctgacga aataaatagg 6060

tataatttcc caagggatgg tgaattatct gctatctaat tgtgtaatca accctcttat 6120

tttctagagg gttttaccct agtaaatttt aactcgacat aaaaatcatc tgaagtaaac 6180

aggaaacatg atcaactcga tagataaatt atttaggtgg aatgtctttt gttttcaata 6240

agttacaaca cgtcgatagc caaagacgat tatcgttttc tagaatcagg gatcggttaa 6300

atagattatt taagcaaatc taaatctcag caccaactct gctggaatga attattagat 6360

tttcgaatca ggatgaaatt tttatagagg agtggatttt gatttgatac aatcaggaaa 6420

taatacaaac ctttcttgaa taataatcat tcaactttgt aaataagaat cattatcaac 6480

ataaattaca agtagctccg gtgtttgtct ccaccgttta acagtgcctt atctgatata 6540

aatcatatca catagaagtt ataaagcaat aatcttccct tgataatgtg atataagtca 6600

caaataataa ccttatagaa aattaaatgt ttacatgctt ttataaatcc tttatattgc 6660

acacaacatc acatactaac tactaaggtt ttattatgac ttatcgtatt gcagcctgtt 6720

gtcaactcgg tgaacttgtc actaaccgta aaggtgaaac agtatttcaa caatattcag 6780

attgtaaaat gggaactatt accgttaaaa gtatgcaatc tttatcacca acacaacaac 6840

gtgcaaagtt gttaggtaaa gtaaagcaaa acttgcaagc cttgcaatcg caaatcaaac 6900

gtgtagctaa ctttcctgat acgcaaaaga tgtttcgcat cagcagtgac ttactacctt 6960

tatatacaca tcaagatttc acacatcttt atgatgatca aatgatgcaa gttatcagta 7020

aaagcttatc aatcactggt aagattatca aagatcatga tatccgtgta agctctcatc 7080

cttcacaatt tacaacttta tgtagtgaca aagaacatgt gattgataac tccattcgtg 7140

acttagaaca ccatgtaatg atttttaatc ttatgggttt atcaccggat gatggtattg 7200

tcatcaatat tcatgctaat ggttcatcgt ttgagcttcc agaacgcgca aaacatatat 7260

ttgactatat ctcacttgaa aacgatgaga aaaaggcggg acatgacaaa gtattaatgt 7320

tgtgtgagaa gtacggtatt cgttacgtgt ttgaccacca tcactattat tgtgagacag 7380

gtgattattt agactttaac ggggatgtgt ggcaacgtat aaaagctact tggaaaggtc 7440

agcgacctat catgcatttg tcacaatcac gaggtgatac aaactttcgt gaacaatgcg 7500

cacattctga tatgatcaca gatcaagaat tgattgagta tatcacacct tatctttatg 7560

aggcagacct agacgtagaa gcaaaatgga aaaatgcagc tagtaaccaa cttgatttag 7620

acgttcaaaa gtgtgaagcc gatcacgcat aaagtttaaa ataaaccttt acaagctgcc 7680

aaggatcggt aataattacc acatcaagac aaacacaatg ttaacaaaaa ggtaggacgt 7740

tatgaacaaa caacaattgc aatcaaaact aaatgagtta ctatcgagag gttattctct 7800

taatgatgtg gtagtagaaa tacttttcga acgtcttaaa aactttaaat aggtgtaggt 7860

gatatcatga cagcatcaat gattacagct caagttattc tcgcatgtat tgtgttttac 7920

atggaatata agcaaggtaa ataatgtgat aaatgtcacg tattacgaaa gggttttatt 7980

gacaagtgaa tagaatcctt tacaatgtga aacatagaca acaaacaaag gtaaattgct 8040

atgagtacaa cttacaagat cttaactaaa ggtttaagtt taacaaggtt tgcaggtggt 8100

aactttgaat gttatctaaa tatccataag tatgtgagtt atcacgaata ttattcttta 8160

aaacttaatt accgtcaagt tgaacactta aaagagagta tgataaactt tctacgtgta 8220

agtgattcag atatatacga actttgttca ggtttcaata tcacacgcag acaggttaaa 8280

accttgataa ctgatttaca tatctacttg aacgacccaa tacataaaag ttaaaataaa 8340

ttcaaaataa cccttgacac aaacaacaaa caagtttaat attaaacacg tagaagagag 8400

aagcatctct caacagtaaa caagataaca gtcaaacaag ctagaaggtg tatcctatga 8460

caaagatcga agcggttaaa ctttacaaac aagaaaatac agactttatt aaagactgtg 8520

aacaacgtaa agacagtgtt atgttgcgtg aagattggaa caactggaca gatggccttt 8580

gtaaaagcgg tgttattact gagaatcagt ataataagtg gtccaatcct ttttaacgtg 8640

atacaaatca caaattgatt ataacaggga atttttgttt gacttgatga aagttcccga 8700

ttatatttaa ttacatagac aaggcaataa agccaaacat actgaaaaag gtaaatacta 8760

tgtcaaacca aaaacaaatc aaactagaac ttttcaaaat tgttaacgat tcaatcgata 8820

ctgaatgtta cacagatcaa aaatcctcaa cagtagcgga aaagtgccaa tacgttttaa 8880

aatgttttga aaaggagtac tatgatcgcc gtgaatcaaa ctacacaaag cggctagcgg 8940

gttggttaca aggtttacca tcaattatga caatcccgtt ttataatggc gagattatcg 9000

agatcggtta taagctagat cttatcaaga agggcaaacg tcaaaccaca acagaaaaca 9060

atgaacaaaa gtttatagat cattggttcg aattaatggc tcacaagttg atccagatgt 9120

cacaaatgaa agattatgct gaaaagcaat ttaatgatta cattgttaag gagctttaat 9180

tatgggatta ttttcatcaa acaaaacaga acaaattgat caggaagtag ttgataagtg 9240

tttaattggt cgatacaaag acacagtgag aagtcatcta tattgtattg aagagttaaa 9300

acgcgatgat ctttctagca ttgaacgtat gacgctggaa gtgaataaac aaaaaactgt 9360

cattaaagca atcgagattc ttgatcaatt agaacataaa gatgtaatac cagtggaaga 9420

tgtgtttctt aaacctgaaa aatgtcatac attagaaatc acaaaatacc aaatccttaa 9480

ataacacaca aaaggaaata caacatgttt caaacaatga atgtaattaa aacacaacgt 9540

gtaagcttca aaccttcaga gattgatcaa gaatgggaaa ctaagcaaca gcgaaaagat 9600

aaacgaaaca agcctaatcg acgaaaaggt cgtgataata aacgtaatca attctaaaat 9660

gtgatcatta tcaaaagctg ccttaattgg tggcttttct tttatttaaa cgttgaaaat 9720

ttttccttgt ttgttatact cacatcatag aaacaaacaa tacagctttt gaggctgaac 9780

aatgacttta tacgaagaat ataatccaag ttttaaagtt tttgttgtat gtcatacaga 9840

tttcggttat attacccata gttatataac agctatcaac ttggaatatg caacagaaga 9900

agctattcaa aagtttgtta atattaaaag tataatgcag ttgaataaat aaggagagtt 9960

tattatgaga ttaaataaag cacaacaaaa gcgaattaaa cgagcggtaa ggctttcaga 10020

gtgggcttgc agacaaagaa aagcaaacgt atcagataat agtaaaacgg ctcatagtgg 10080

tatacgtgca gttatttgga gagggtagat acaaacaagt tgtattagta gctaggcgtt 10140

actcgctgcc attcatagcg gagttattca ttgataaaac gtgaccaaca tcacaacaaa 10200

acatttgaca gataacagcc agtaagcaat aattaacacg taaacacaaa caatcattta 10260

aaaggtaata gatcatgaat acttcagaac taattaaaaa gtttaatagg ggtaacacgt 10320

tgaccaataa cgaactgcta gatctctacg gtgaattatg tcagattgaa gatgtgttga 10380

ttggacaggg tgacatgttc tacccgacaa ggctatttac atcaaatatt aaccaaacag 10440

ttagaagagt gtgtaaagaa agaggtttga tttaatctat aaggtagatc acattttcta 10500

taagtcgtta tatacaaaca ttagcggctt atgtaagatg aatctacagt agatattaac 10560

caataaaggg taagttatta tgaaaacata caatgaagat attcaccttg tctatacaac 10620

acattctttg gttgttacct tcctttttat tgcaaaaaca cagaatattg aattatttat 10680

ttatgataaa acacatgaag agtggaagga attatttact cacactgaag aatggaaacc 10740

ttggcactat gaaaccctgt ttagtcaaaa acgatctatg ttaggtttag tgagttctat 10800

aattagaaaa ggttcatact atgaaaacac aaattaaact tgtatttgaa cacgaaacac 10860

atgtaaaatt aattgagttt gtaacacatt tagaatcatt atcagacgtt tgtacatttg 10920

tattagctgg agaagcttta ttacttgaat atgatgtgta tctgtatatt actattcgtg 10980

ggcagattgt taccaacaca ataaccctat tattgcaatt gatcgagatt gtcagcatga 11040

agtgttaaca gattgggatg ttgagcaatt ggaaatgaaa agatttgtta tgtgacatcg 11100

atcacagaca ataatcaata actcaattac tatacaaaca catttataag ataagagatt 11160

aaagttatga gtaaacaaaa taaaaacaac acatttcatg caatattatt cattgtgtta 11220

gtggggttct ttttatgggt aactaatcca agtgatgcga taaaggattg tgagaaagtg 11280

aatacacaag aattttgtaa tgttttgaat gctgagtgaa gatattgaat aaacaaagga 11340

gaataaaccg tgaatgagaa attaagtgat ttatcactag agttaggaga ttatttagaa 11400

aacaagcttt tacctgttta tctagaaact ttatgggaag agattgaata cttaacttta 11460

gatgagccag agattaaagg ttctatagta aacttaacat tagcaagttt ttcttgcagt 11520

attaaaatta tgctagaaaa gagagaagac ggttattata attcaatgga tgatttacat 11580

ctaatgtttt atttaataac gccttgtagt gataaatttt ctttctgtca tccattacag 11640

atcaatatgg ttttacaaaa agagttgtta atgaaggtgt acgaagtaaa taaggaagct 11700

tttgacaatt taccttttaa agtggttcta tgggattaag tataaagaga taaacccgcc 11760

atagttggcg ggtttttgtt tatgtgttag ttaccattta ggataagaga ttgcacatga 11820

ttgatagtta taataactaa actctttgtt taatcttttc cactcttcaa aacctctttg 11880

ataataacta tcattcaata actctttatc ataagggttt aagtttaacg ccttgcagta 11940

attaaaccac tctttatcat ttcttgaata cattctgtta tccttggatc atactctaac 12000

ttaactgtat atatgtttag tggggtttta ggtttgaaag tatctttgtt tgttggttta 12060

taatcgcaat tcgaaacact gttaatagtg tcaaaccatt cataatcata cttatgtagt 12120

gtgttaccat ttaattcatc tagtctatag taaagagaat tattctcatc ttcattagtt 12180

gaatagatac atttagactt cttacggtaa caacgatcaa agcttatata gtaatttgct 12240

ttgttgtcta tctcatgcca attgaaaggt ttcatttctc cagtatcagg acatataaag 12300

caattcaact ctcggcataa cctagaccat tcataaacct ttttgatatg ttctaacgtc 12360

cataacgcac caataaacca acgatcagag tttgtataac tgataaatag aaacttaggg 12420

gataacataa caactggttt tacattcaat gatccgtcac tgttatactg tttgttaata 12480

acctttttca ctaagttgtg tttgtttagg gttctaaaca gcctgtccac ctttgaaggg 12540

ctaacgctta ccttatcagc aagcttccta ttacttagtg agaatgtatt atctcgccta 12600

tcaaaacact tcttattgtt aatcaactgc attaacaatt gattctcttt gttagttagt 12660

atcaatcaaa accccctcaa atcttattga taattgttct catataatca ttataacaca 12720

ggtgtgtaat tacttgaaat gacgcagtgt gaaaagcttg ccgtcccata tacaacaagg 12780

gtttgagaat gattcttgtt tacaaattcc ttatactaat ctatctaggg attaaatata 12840

taatactctt tatcaaaggt tggatctgtc ataaataata taacctcttt aatgcggagc 12900

attgggacaa cacacaatgt aaagcggtag ctttgaatag tcatacattg acaaacggta 12960

atcagtggta agaaaagata gttattgtgt aagtatatct ttaattaaaa cgaagttttg 13020

agaagtttgt tagataaagc gagagcttta agggagaatg tttataacgg agttatgaag 13080

gattattgtt gtggaatggc aaggttagat tgggggaggc ttataagatt tcgatagaaa 13140

ttgttaagca aaccgctatc gctcttacat cacaaccaca tcaaagctac gctttaaagt 13200

atcaactacc ttttaattgc acagcaattt cacataacca cctttgaatg agaatcattc 13260

taataataat tcacattctc aacaactgat taatattcga tgcgaagcat caaatatgca 13320

gtactaaatg ataatcatta tcatgtatca gcagcaacaa atgtaacttg taagagactt 13380

gagaatagtt ctcaactgta gatgtgacaa gggatgcgga gcatcgaagg atagtgataa 13440

tgtggcatga tattttggat tcattagcga ttttaggttg acagccataa ttcacgttaa 13500

gtagttctct agcggaattt tagatatttc ccatatcaac aggtataccc ttacactaat 13560

aactatacct cccaaaaatt tttattattc ttctcacaga tatttctacc tccaaaatcg 13620

actagtttta cttcaaatta atagctgttt aataaaaatt tattcactct ttgttgtaac 13680

acaacaaggt atacataact taatcttcca gattaacaga acacagtgtt ggtaagggtt 13740

agatatttgt atgacagata tttcccagag gtggtatgta gcaatgggta tgtcaaatat 13800

tttaggtagg aaatttaata agaagaggta gtaattgagg taagttactg gtattttata 13860

gtaggtgtga tctaggaggg ataatgttac ttgtaacata gatttgattt tataagaatg 13920

atctttagaa tgttacacgt aacatataaa atgataagtt tacttggtag tggagggatg 13980

aatgctataa atcttattaa atacgtttta atagggggta ttcgagctgg gaagctctat 14040

gagaggtgtt agggagatag aggtatagtt ttgcctagat ataagaaaag ccccttagag 14100

gggcttatat aaaggataat ttataaaggt attatagtga ttgttttaca cgttcacgga 14160

tttctgaaag agaggtttct cgtagcagtt ttccatcttt gaatacagtt tctaacaacc 14220

cttgtttttc ttgttctggt gtttgctgat catacactac atacttaccg ttttcttttt 14280

caatacgtag taaccctttt gcagatttct tcttactgtc cgttttaggg tctttgaata 14340

catctacacc tttgccagcc ttttcaaccc aagttgcttt tacagcagaa ccatgtgtat 14400

cacgagttac gtattgatat gagtaactac caacaccaag aacgaccata gatgcatagc 14460

ctttatcttc tagacgttta ataatctctt tctgacgttc tagtgtaata ctgtcaccgt 14520

agatagctcc gatattatcg tgtagaagtt tcaatcctgt cggggataca gttgttccaa 14580

agatatcacc gagacatacg ataagacctt tcacctccca ctcagacact ttttcagcct 14640

cataaatacc atcactatca aacatactgt catcataagg ttttttgtac caagattttg 14700

tcttatcact gtattcaact tcatcctcag tatagccagt aagcactctt acaggatcgc 14760

cactatctgg acgaacaacc agtttaccat cacgtttaag aatatcgtct ttcagcatag 14820

gtaacccttc actcacaaac ttccagaaat caaaactatc tgatacatgt gataagatac 14880

ctgttggaac cttatccatt aagtaacgaa catactctac ttctgcttct agttttgtaa 14940

tacctttctc ttctgccata gaagtgatgt aacttgttgc tgtagagtgc tccgttgctg 15000

gcactgatgc accaactaat tcattctcta catttgcatt ataatatttt tcagcaaaca 15060

agatagctgg gatcgtatct gttcctgcaa aactacatag gtgaccaaat ccactcatag 15120

ctgcagcatg tcgcccgccc ataccacgca ttgagaagtc gtgacacatg aatggaacta 15180

aatcgtgtgg caaacctgct cggtcaaaag caccaattgt gttgcgtagg tattcacgag 15240

ctgttgtagc tgatgtttgg attaaccaat tctctgttga gaaagaggtt tcaatataat 15300

tgctcagcca ctgaaaacca tctactgtat tcacgaaggt tactggtgga acaccataag 15360

ggacactaac accttctggt agagctttaa tctctagagg taaataacct aagtcatgca 15420

actcctcgaa gtgtgaagca tcataatcaa aaccgagaca actatcagta atacgtttaa 15480

tcgaacgaat agctgcatgt tttggtagat taaagaatgt ttcattccac tctttaataa 15540

gaacatcttt gatgaagtat tgtaaaccta caaatacaac ttccttgtta tctttgacat 15600

tacttagccg tccattacgg gatgtgtagt tagaatacac cttagtgata tctgggtggt 15660

aagctgattt gtggaacatt ttgtaaaaat ctgtttgtgc ccaacttgca ttaattgtca 15720

tatatttctc ctatttaaaa tttgaaagtg attttaaagc cttttcgtaa gaagtgtgaa 15780

ttgtttttgt agtggagtac caagattcat aatagccatt atctagtttc ttatattcca 15840

caactttata tagtaaacca aagaacaatg gttccacttc aatctttcgg tcagttattg 15900

tcattaaacc tccaaacatc ttgttcatta atatagttac cgattatatt agaacaccag 15960

ataaaatcaa catctttttc taaaacttct aaacctttcg aaaagatacc gtgtgttaca 16020

aacaaaccta ctttatcagc acctttctct tttagtttct gagctaagaa tttgaaactg 16080

gcagaaccat cgcagatatc atctagtata agaatattac cttctacctt atcgactgtc 16140

aagtcacatt ttacaatatt acctgtttta acatcacgaa tcttaacagc ttggatataa 16200

tcctcatgat ctaacatcat tacactgtca aaaatctttt tagttgcgcc taaatctggt 16260

gcgcacagtt tgaagtttgg tagtttacgt gaaatctctg tcaccttgtt acgtaatagt 16320

tctgtttgtg taattacttc tacattatca ataagagctg atgtaacacc gctgtgtggg 16380

tcataaatgt aaactttact gaaaccaaaa ctattcaaga tacctgcaaa taccttagat 16440

ggtaaaggtt gtcctttttg aaatacacgg tctgctcgtg cttgcgggaa ataaccaagt 16500

gttagtttaa catctttaat acctagccga tccaaagcat ccttcaccaa cccaagacga 16560

atcaaatcac gagtaccatc ttcaatctgg atttggatat gattaagacc atcaaactct 16620

aaggggatag tccatttatc tttcgttcca tgtttgattg tacaggtttc agtaccatca 16680

ctgaaagtaa cccactcaat tgttgcacaa ccttttacaa atgtaatatt actcatactt 16740

tctccttctc aatcatcaca gccttaacat taacatgacg actatcaatt tcctctacaa 16800

ccacattgta atctacacca actttgtaaa ttagtttatc accatatgtt tgttcaagtt 16860

ctttcagtcc gaccaccagt cgttgcaaca tctcatcact attgatttct tttcccatga 16920

acggcattac aatgtgttta taattgatac aagtaacaaa atcaatataa ttgttgataa 16980

catcttcttg tgttgtgtaa ttaatcataa gttgtttctt ccttgttgtt ttatattctc 17040

taaactagcc attattctat cttggaattg tctctccctt tcttctttac cataagtaac 17100

atacatattg gtgtaggttt ctagaatacc aagaagaata gggacacgtt caactgtttc 17160

ttcacacgat agttttgtgt ttatcttttg ttccgagaag gttagttcta tatctatcac 17220

ttcattaaat atacgactac tgtcaggtgt gatctctacc gtgtaaccct ctcctgataa 17280

taaactttcg tgttttattg ttagtttttc aaaataacgt atcatacata ctccttcctt 17340

caaaacctct tattccattc atccttcgtc atcaccactt cataagtgac atctgtcccg 17400

ttccattcat aaactaaact atctacgaac ttagccttat tatgacctat cagtgcagac 17460

ggtgttacta agtggaatcc tttatctaga tacccatact cccacaagag atagtttata 17520

tattcacaat ctatccatac atccctgcaa ggtttaggtg gcttttctaa agcaagatta 17580

cctgtcgctt taagtttaac ccttttccag tccataacac aacctcttaa aagaaacttt 17640

gtcttgctaa acttaactgt ttgaaggctt ccgtaagaga gtgtgtatga cgatcaacac 17700

aaacaacatt attcattctc tcataataat cttcatcacg aatctctgtc ttagatgtaa 17760

tcacaccttt atccacacca cagaagaatg atgtttcacc atgtttagct cgataatcta 17820

gatggacaac atctgtaagc tgtaatgtaa acttgattgt gtgatgttct gtcattgtac 17880

ctacagtaat ctgtttatca tactcaccag ttacactctt accatcatca gctaagatga 17940

agtgtttcac tcgcggaatt gtgtaaactt taccattatt gtatgtgata gtgagttggt 18000

ttgttgttgc aagtttctgt ttagtcatta agttttactc ctttaagttt attgtctatt 18060

cttgtcttta attcaagaac ttcattgtaa agactttgta cataatggta ttgtgtttgg 18120

tattcttcac actgcttaaa atcctcatcc ttttctacta atgcatacac tgtgtattta 18180

gtagtcaggt tttcataatc atcgtaaggg tcagtattta catcaacttg tgtcttttgt 18240

aaatctacaa acgtccaacc accattactc tctttgtcat tgatatagtt gttaatctca 18300

ttccacatct tagcctgata gtctggttga tcaccgtaga agtaatcctt aatccaactt 18360

ttacatggaa taggtgctgt agctttatgt tctatatctt ctaactttct gcgctctgca 18420

ttataacggt ctacactttc cttaaactta gtatagtcat attcgaacca gtggtcatct 18480

aattttatct cccaaggaaa ttctttcata tcttctctcc ttaattatct aaatacacaa 18540

taacattatc tggtaaacac tctagcattg gtaacacatc ttctacactt ttaccaccat 18600

gacttactgc aggacagggt agatgaaatg tccaagtagg acgttcttca gcgatacgtt 18660

tgagtttatc aatactatct ttcacaagat aagttggtga tggttttctc caatcaatct 18720

tagtttggaa tgcaccgatc cattgttctt tccattttac aaaagcgaca ttgaactctt 18780

gccctgatac taccttatca ccaaacatct tgtcaacacc aatatatgtg tcacgagcag 18840

attttgcaca tccagcaccc atgactagct taccatcttg tttgacaaca gagttggctg 18900

taaacatata aatgttatta ccaacaacac ctcttgggta gtataaacgt tctcttttga 18960

gaatcattat tcctcctctt gttgtatata acctaaagct ctcaagacat cttcacatac 19020

ttgaacaaca taagcttggt ctgtaatgtt aatggtttcc cactgaaggt atatcatgct 19080

ttgacaattg tggtcaagtg atacatcaac agacggaagg tgtttcttca tttcgtggta 19140

aatgtttttg tgtttacata caatcatatt tcctccaatt caacatcaac aacaaactct 19200

cgattaccaa gagacttaat acttacctta acattgtaaa cactatcata attgtcacca 19260

agatgctttg caagaatctt acgaacatcc gtgttaacat tatcacgagt taagtgacaa 19320

ctatcgtata acatttctag tgtgtttaca atgtcggatt tgattgtttc tgtgttatct 19380

tgcataaaaa taaatctcca cacctaatta agatatggag attatgaact aggttttact 19440

tgttgtcaat gtttttattt aatccttatt catcttatct acaaacttaa gaatattaga 19500

aatacgttca atttcttcac cagtaaactt atattctcca atcttaacaa gtgtcacatc 19560

gtgttcttcg cttgtataaa actcagcagt aatatcttca tgattaacac catctacacc 19620

aaacgattct agtgtttccg taattttcat tttacacctc caataatatc tacgttttta 19680

taaaatgtag ttttaccaac accattaact tgtttcataa ggacaatagt ttttacagtt 19740

ggggttgttt caataaagta atcaatacaa ttttcattgt aactacgggt ttctcggtat 19800

aaggtgaaac ctttacgagt gatttcagct tgccaataaa ggaattcttt ttctgtgatt 19860

gcaactgtgt tcataagatt aatccttttt cagtaaatca ttagctgact ctacgacagt 19920

gttataacgt tcacgaagat gcttatcatc tttcacatca atccaatact gcgttaaaag 19980

ataattgata catttacata aacgttgttt atctgttgtt ggattaacca aggcatcact 20040

gcctgcgata agttttatac tgtctttgat atcgttcatc atacctcatt attcttatac 20100

ttattaacaa aggcagcttg tgaagggttt aggtaacaat ccttcccgcc attgaacaag 20160

tttttaattt ctaaatgttc tttattatat aaccaaacac caagatagtt aaatttattg 20220

tatgcgttta aatcatccaa tcttgtcttt tcttcactgg ttaattctat ccacccaaat 20280

ctataaagct gttcttcgta agtcataaac atattagagg ttttgtcttc taaagttctg 20340

ttaccaacaa ctctatccca taaagacggt ttaaatctac tctcagcata agcaatttta 20400

tccctaagca agttgtctgc ccactcaact tctttttcgt agcaatctaa tgctctttta 20460

atttcctctt ttgtatataa agcagcacca gtgaaaatac tcatctatac ctccaaatcc 20520

gcttgaatac aaaatgtgct ttcatcaaag tgttcatctt cccactcaaa atgtgccgat 20580

tcgattaagt aatccatcat aatatttgta atgtgttgtt cttgcatatt aatacctctt 20640

ttatttaata cgttgtttat ctagaatctg ttcgtatgtg tcagcttcta ctttatcata 20700

tcgtggacgt ttgtaaatag gtaagaacaa actttcttga ttagttttct tatctttcgt 20760

gatgctatcg tactcaactt caataagggg tggttgctct tttacgtaaa gtttacgttc 20820

ttcttcactg aaaccacttc caacatcaac tttaattgtt ccacactcag attcacaatg 20880

gaagccacct aacatccctt catacttgct cccaacttct gcataataaa aaccaattac 20940

cttaagatct gctggctcct tacgttttac ctttatattg aaagcaggtt taccaacatc 21000

tttccatgta gcatccatgt ccttggcaat cgcaccttcg taaccatcac gaacatattg 21060

ctcgaagact tcaaatactt cttcaacaga tacgttttct tttcgttcga tgagatgaat 21120

tttaggcgga acatagttaa tagagcacca gtagtggtat tcattcatca taagctctaa 21180

acactctcgt cgcttcttat tactttctgg gtactcacct tttggttggt aatatttagt 21240

atcaatacag tcccatactt ggagtatagc attctctttt tcttcttcag aagctgttcc 21300

ttttacgatt tttgtaataa tgccgttacc actctcacga gtagctttat caagagagta 21360

tactaactca ccttcaagag caaaaccatt gaatgcacca caagataaat gctcttgtaa 21420

acattcaata tcaagagggc taccattacg tgacatcatt gtagattgtt caccacaaat 21480

acttgctgca taactaccgt cggattttag ctcaacagct aggtttttaa tagaatgcag 21540

tttcttcaat gacttttcat tcatagaagc agcacccata cgaggtggtt taacaattaa 21600

acctttccac actttgttaa tggttgcatc tttcacacca acttttaaat cttgtaatac 21660

tactttctca agaacttctc ggtcacactg ttttgtggta aataataatt ggtcaagata 21720

ttgtcgggct tcgttacctg tgtaaatgcg gtcagataaa ttcttaagta aagggagtgc 21780

ttgttccaaa gaacccagtg ctgttggtaa tgattccccc ttttgtaaat tcttaatata 21840

gaacttaata gttggtgagt aagctaaacg gaaacactct ttcaaggttt cattatcttt 21900

atgttcaagt aagatagctt ctttctcatt cttactgctt gttgcagcca cttcttctaa 21960

aatttggtaa acttctttcg tcattacttt atttctcctt cttagggtaa aactctttag 22020

ggatttgttg attactactc caatcaaaac tttgaccacc cacacactta acttttataa 22080

ctggtgtttt accgaacata ttactataag tgtatacacc accattatca ctgcatacat 22140

aatccatctg tgctttctgt aaactattag gttcacaccc tgttaaaagt aataaacctg 22200

aaacgattaa aactttcttc atactcatcc ttctcctaaa tttctcaatt gatactcatc 22260

tgatatttca tcaacatgga ttagttttcc tacaatctcc tctggtgaac cgaacccttt 22320

atcacctttg ttatcctgac agtggtattc tgtgtgatga tatttataac ttccttcaac 22380

gaagaaccat ttctcgttca cccagtaatc atctaaatga taaaactctg tagtcatatt 22440

gtcaatcaaa tatttaatct tttcgtcaga tagttgtttt tacgttcata aattatttat 22500

ccaaataggt tgaatttaat gcagtaaaga ctcccaccac taaagttatg gcacaaatgt 22560

ggaatactcc ttgcggtaca atattacctt gatagctagc tacaatcatt gttacaaatg 22620

ttactactga tataaacaaa gaaattaaac ctacaacacc gtttgtatat actaagaatg 22680

ttttcataat tatacttcct ctaataaaca aaaatcctca tcccgaagga caaggataat 22740

ataaggttat ttgttcaagc tgtcaacgat ttctttcact ttctcatcaa gtttttgtgc 22800

aagagtatct tgacctatat tgtgtgggaa tttcacacca agtaaatctg cgtaacgttt 22860

tagatcaggt aatccgaatt catcctgtgt ctctacgaaa gatgtgaatg gtggttcacc 22920

ttccatgtgg atatccatca aacctctctt atcaattgtt aggataaact ctccctcttg 22980

aaaagtctct ccacaaactt caaaaacatt atcatctagg acacaatctt cttcagatac 23040

tacctcacct tcaccaaaca gcatagctaa gatttcttct ttagatttat cttcaaaatc 23100

ttctttggag tagagcggtt catcttcttc aaactgtttc atgaattcag attcttcctc 23160

ttggaaaagt tccatacgtt tctcaagacg ttccatgatt tcgtcagcat ccatccaaag 23220

gtctttgcca tttaacactt ctgaaatctc atcttcagac atgaagtgac ggtagtactt 23280

tttgataaga cggtctgttg cttttgcttg atgttcaaca tattgacgga cattattttc 23340

ttttgctaca tacccatatg tgcttgtgtg aatcatcatc gtagcatcat ctgtaacacg 23400

atattcatca cctgctaaga aaattaatgt agctgctgaa cacgcctctc cttcaatctc 23460

cgtaataata tgagcttgtg attgagccat actattaagg aaagcttttg ctgtatctaa 23520

acgaccacca ccagagttaa tcagtagatg aacaacatca ccttctttgg attgacggat 23580

tgttgctaac tcttcttgga atagggaagg gtcttcaata tcacggtcaa cacggattgt 23640

gtattttaca ttaataggtt cagcttgggt tcgaatgaaa ttaactggtt gctccatata 23700

ttatctcctc tttataaaaa cttatctaaa tacacttcaa tatcatcttg taagaaaaga 23760

taatcatcga tgtttctgta tactgtttca agtgctctca tttcatatga attacaccac 23820

acttgattct tactaatctt agccatcttc ataacatcaa ccacaacttg taaatacttc 23880

aactttctct catactcacg gaagatttga gattgctctg agaagtattc taaagcgtct 23940

cctttcattg tccacttaca aacaggtttg acagttttac gtttcttcca ccaagacagt 24000

ttattgtaat tagttacttc tactttccac tgctctttat aagttttatc aatacgttct 24060

acaactgtgt cataagcatc ttcaatctta cgagtgattt cttgtggtct aggaaaatca 24120

ttaacttgac ggaagaagtg atctaagtat tcgtatggaa ctttaccttg cataattatt 24180

actcctcttc cattgcagat ttcgcacaac caagtgagat taactttttg aataattctt 24240

catttgaata ttgatcaaga ttctgagatg acataccaag ttcaatacaa gaatattcta 24300

agctttcacg agagtaattt tcccaaggtg tgtatgaatt tgttccaaca ttaattttat 24360

aatttgacat aacattatct ccgttttgtg aatgtagttt aactatagat taatattgga 24420

actgtgtcaa gggtgggtta gaaattatcc tccacaaatt ctgctacacg agaacgaact 24480

acaccttcta acctcataac cttagcgaaa gggacgccgt gaaacttctc tgtagcgtaa 24540

gttaaccctg atgttcgtgg acttacgaaa tcattatcaa tctgccccaa attacccatg 24600

ataattactt tacagtactg acctgcacga gataggattg tcttagcttg agatgctgtg 24660

agattctgaa attcatccac aattaatact ttatggttaa cagaacgtcc tctcataaaa 24720

ttcaaactct taaattgaat gaatgcatcc tcattccctt cttccatatc aattgctgcg 24780

gacggttcaa atttcttatt tttactttcc ctcgcaataa tctccatatt atccgtacat 24840

ccagcaagcc atggccccat tttttgggct tctccacctt ttaagaaacc gatctcttta 24900

aattgagtat cattactccg tgagaagata atcttttcca tacgataaga ggacttttta 24960

cccttcacca tttcacaaga cgctgccatg gctaataagg tcttaccact accagcttta 25020

gcatcaagga tcacgatatc gatttcaggg ttgagcagtg catctaatgc aatagcttga 25080

tgtacatttc ttggtttaat acctgccgct ttacgagaca ttaaatgatt gtctatatta 25140

tcaaagcgga aaatacacca tttatcatcc tcagtataat ccaccagttc agcagctacc 25200

ccatcatcct cgttaattaa ccaatcattt aacgcaatag acgctgtttc atcttcctct 25260

agtaattctt gaatacatga tttagctatg tgtgtttctc cgcaagattt agaaatcact 25320

ttatctgtat ctagagatga taaccatcca tctggtacat ttaggtaacc tttagggaga 25380

aattctgggt cttctacaat aacatcacta ctaaattctt gaacttcttc aacacctata 25440

cctttcgctt tcagtctcat acaaacatca ttagatacaa gaatagtttg tgtgttactg 25500

ttctgcagag ataaggttac attaataata tggtcatcat tacatgtcgg ttttatataa 25560

gaagtatcat cagttacatc aatattctta gcaacaaaga gtttacctcc ggtaggtaag 25620

tctactccat gtttaatagg atctagatta ccaattactt catccaattg tctaatagct 25680

gctcgtgcat ctcttgcaat atctctatct gaacttttct gtttatcgag ttcttccaat 25740

acagttaaag gtacaaccac attattatct tcaaatttta acagagaatt tgggtcgctc 25800

agaaggatat tagtatccaa tacataatgt tttaccatgc gcacctcctt agtgttattt 25860

gaaatctgtt ctattaacca accaacccca tacaaacttc tcatctttac ttcgtctagt 25920

agctaagtcg atatagaatg caccctgttt aatatttaaa gcattatata aaaccttttc 25980

tcctttctta ccgcgtttat tcaaaaaaga tttcaatgct gagcatgttc ctttaccaat 26040

aataccatct tcactgatat ctttatagta agcttgattg ttgttgaaaa cgtttaacaa 26100

ctcttgtaac caacgtgacg gtcttgaggt tccagtattt actcctgtgt caaacaactc 26160

ttgtgcgata agttccgatt ctttagcgac gaggttaaat cgagggatat agaaataatc 26220

ataagcgtaa atatctttag cgaattcaat cggcatcgtt ttcatatccc cgttccagtg 26280

atataaattc caactacctt taaacttaag agcggtttta taagtaatac cccaattggt 26340

ctcaccacct gaatcactag gatcgtctac gtaaccaccc tcgtgtttag ctgttgtcat 26400

gataaagtct ttaacaaact ttggagattc tttgtaacac ccaataatac tcataattca 26460

accttctcct taaaactatc aaccattcgt aaaatctcac gaattcgccc aaacaattgt 26520

tgttcttctt cactatcatc tttaaacgta gagaagaagt aatcacctgt gtcaccaaga 26580

ttatctaagc gataacctcc atctttatga tttacaaatg tgaagtgttt accctcttca 26640

tacagaaacc aatcctttgt ccgaaacatt aaattttcca taaaatctat cctcctattg 26700

tttgatgaat attagtttat ctaacggttc acacttttct aaaacaatac cacaataacg 26760

tttcagcatg gcagaacaca ttttatcttg tggacaaacg agggtgaacg tttcatttgt 26820

tgtggtattg tgtgctgtaa gtttatcacc tttgttgtaa tactttttaa tgttagttta 26880

cctcttggaa tagatcgttg aatgagattt cttctctctt gtttacccaa ctccctacaa 26940

actcactgac gctgtcttca taagactccc agtagacatt cgaaccccaa tcaaaagttt 27000

cttcgttgta gcaagggaaa ttgttttgaa gataatcttt ttgccaatca gatagtttac 27060

ttaattctag gtaatcacct tctttcaatt tataatctga gcgtattaat tctggttctt 27120

caacttcctt cggtaacaaa tcttgtggtg ttactttgtg gaaacggtct ttatgtaaac 27180

taccttcgat acctttgtcc ataataattg tataattatc attgtttaca ttaacaactt 27240

tataatcctt accgagagtt acttgaccta aagagtcatt acaacacgtc acccaacgag 27300

gtttatcaaa gattgattct tgtaagaatt ctgtatcatc ttcaccccaa aacatccccc 27360

agtagaaatc gttgaatgtg atttcattct ccgaaggagt gcagtattca gttgttgtgc 27420

caaaacaaga tcctctggta actttaaact ttctataacg ccaaaaaggg aattcaggat 27480

ctctaaactt atctcttgat gatggtaaaa tatccagatt ctccaaacac cattgctttt 27540

gccaatctgt taactttcga acatcgaacc actggccttc tttaaattga aatttcataa 27600

ctttctcctt tatctttgtg taacatacaa cgtccatgta agtatgtatt agtttgtgtt 27660

aggtgtcaat ggtatttatt aaaaatctaa atcgtccatg tcacccatat caaaatcttc 27720

tacatcagtg ctcacagcac taaccatata gtttgttgga tcggtttcca ttggggcagt 27780

ttgatggtta ttaatattca tccaattatc ttcaaaccaa ggtagtgggt ttgtaactgt 27840

tctgcggaaa tcttgtttca aaccaaggtt gtcatacact tcttgagcat tgtaacgcac 27900

ccagtcattt aacaaaccct catttaaccc taccattcga cgtccttcac taaacaagta 27960

tttattccat gccaattctt tagtgacaac atcatctacg atttgtttaa tctcattctc 28020

gtataagtgg aataccattt tccactctcc ttgagtgagt aggacatctt gcagagcatg 28080

tctctgaact tcaaaatgaa cagtttcatc cataagaatt ttttggacta gtcgagcagc 28140

acctacaaaa tactcttgct cggctaacgc aaaagtagct gaaaaactag acatgaaact 28200

tatacgctct agtgcataaa ttgctacaac acctttaagg atgtaaggtt tgcatttctc 28260

aaaatcccac tcacctaaac tataacgtaa tccagcctct tttaactcac ctaacgcctt 28320

accaaataca tactgtctat ctaaaacctg ctcgtgctta taaaccatat cgaaaacttc 28380

ttgtgggtct ggaatacagt ttcgaacgat agatgagtaa gtttcactat gcaacatctc 28440

attctccccg atacgggcga taacgtgccc atattcccag tttgttataa atgggttgag 28500

aagttgaggg atacttgttg ctacactatc taatgaccac tgataagcta gattataaag 28560

catgatatta cgtgtattgg tatcacaacg gagtaaatcc attcgtgtct gagataaatc 28620

cacatcatca gatgcccaat ctatctgtcg taatttttcc ttgagattat agaagtgtgg 28680

gtatttcatg ttaataccgt cgtaaaggct aggatcttcg ccaccgaaaa gtgggtaatc 28740

tcctgtttta tgagctacgc ccttatcatt gaaaatactg ttcttggtca tttaaaataa 28800

ttctccttga aagagggagc ctaaactccc atttaatatt attatagtgc acatccacca 28860

gatgaacagc ctttatcttc cactacttct gcttcggtgt caactgcttt atcagatttc 28920

ttttcctctg aacctgagtt agttcggctg ttaacatagt aacgtgtttt catacccata 28980

gctgtcatat acaagaagtc tttcaacata tctttacctg taatcttctt cttagtgaag 29040

tctaggtaga aatctgcact aatagtttgg tctgtgaacg cttgaaagat tgcataggtg 29100

tcaatcaaat ctttggtttc aatatcccat gcatattcat aattttgttc tttagtgtcg 29160

tagttaggaa ccaagaaccg tgtcatttta gttgcgttag acttaaccaa cttaccactt 29220

cgagcaggta aaatactatt ggtaccgttt gtggcgatac tactgctttc attaggcatc 29280

atggcaacaa gaacactgtt gcgtagtcca ccttgtgcaa taatacgttt acgtagagac 29340

ttccaatcct tactgatcaa gtgaccaaac ttcttcttaa cattattgtt catggtatca 29400

atcggtaacc atccatcagg ccatttagtc ttgtctatgg ctgaacacaa ccctctttcc 29460

tctgctagac gtaaacttgc tttgatacaa gaatacatat gttgttcagc aaccttaaac 29520

atgtattctt tgccttcttt agatgaatat ttaagattta catctgccat atgacctgct 29580

aagtcggtaa taccaatacc aagactacga cgtgcttgag ctgttacctt caaattaggg 29640

aatgggtagt ccataatatc aataacatta tcaatcatta atgctgcgta ataggctaca 29700

tcgtcccatt cctccaaact cacacgacgt gcaacaatag cacttaaact gcataaacca 29760

atttcaccgt tccattcttc tctgtataaa tcgtctactc gattaaaacc gctcgttggt 29820

aaagcgattt cctgaccttt tcagttttga ggtcgctaca cctcttccac gatttctcat 29880

gttgtcggac tatatcatca tccgttctgg atgctctgcg cttcgaaaca ccgatagctt 29940

gtgttcctac ataatagtct ctagaccttc aattaagctt ggctccgagg ttggcatagg 30000

ataattccct tagctttccc agaattcaca gagttattcg acatatgttc ccatatgaag 30060

ctgcatcagg ttgtttacag attactcgaa tagattttat ctttaaatgg tgtgttacgg 30120

tttagttcat cagtccggtg taaatacact tgaccacttt cttgagattg aatcagaata 30180

ctcactgcaa gttttcgtgc gtctactttt ttaacattgg gattatttgc ttcatgctcg 30240

taataaagtt tctcgaactt agactggtca ccttcgtaca ttgcttcata caaatcaggg 30300

ttatcagcgt aagaaactag catccaatct tcaccatctt taaccttctg agcaaataga 30360

cggttatagc cgattgaata gtgaatacca tctacacgga ctgtaattgc agtcttttta 30420

cttttccatg tgataatctc attaatctct gggtctaaga cgttaacatg aactgtagca 30480

ctaccgcctc tcgatgattg taggttagct gcaacagaac tttcaaccat cttgtagtaa 30540

ggacgtttac cttggtgaat ggttgtcccg cctcgaacac catctccctt actccgtgtt 30600

ttgatgtgag atccgattcc agacgaatgg caggtcatca tataagcaat atggtcacca 30660

gaagctaaac tgttagctgt atcatgtgta gtataaacag cgcaactagc gaaaccacga 30720

tctggtgttc gtaatttagt cataaatggt gttggggcac aaatcttttt atcacttaaa 30780

taagtatata attgcttaac atcctgaatt cgacgatctt tgggcatacc tttcatgttt 30840

gccattgcca tacccatgta tgtaaattgt gggctctcta gtgctctatc ctctacacgg 30900

ttacggatca catacttgtc agcaatctgt ttgatttctg gataagaaga tttcaaatcc 30960

ttaccgtggt ccatgaaatt gttcaaatcc tcaaactcat cttcagagta aggtaaatct 31020

tcccagtaac cttgagacac catattatca tgatgttctt tcaaggtagg aatattacta 31080

tagccaccga aagcttcttt ataaagaaca ccagctaaaa cacggcctgc caacatgaaa 31140

tgtttttcat caaaagcctc aacacataca tcaatcagtg ccttgttaat atctgttgtt 31200

ttgcacttat catcaagacg tttataaacc tttaaggaga tatcactcca attaacttgt 31260

cgatctgctg cccaacgact tttcttattt aatttttcag catcaaaagg gacttcttta 31320

ccgttagcct tgataacagt tctaatattt gtcattacct attcttctcc tttagttata 31380

atataattaa tctcatctat ggtaatactt cttacacctg ctgtgaagct tgcctcttct 31440

tttatttggt cttgatattt ttctatgtaa gccttatcac ccttaacgta gagactacta 31500

gactttgtaa attcttcttt tgcaattaat cttcccgctc tcccaccaac tcttctcagt 31560

aggaaatcat gctctttttc cgaacctttc tccaagtatt tattataatt gatgacctct 31620

cttggagatt ttacccacac ctgaaaaaca caaggaacat cgtaatcctc tccgttgagt 31680

atgaaactat ttttaggtaa gtctttttgt aaggcaagag atgcttggtg tgtcaccttt 31740

tccataaaca acactttctt aaaggtcttt ggtaagataa aagctaccac ttctgcaccc 31800

tcttcgaaag aatggttgat aaacttcaaa gccagtgaac ttgcaaaacc aaatggaggg 31860

ttaccaacaa caactttacc tgttaaattt tcaacggtta agaaatcttt cttagtaata 31920

ttactcacct tgggttctag atcatatgct tcccatttca aatctggacg taagaagtta 31980

cctgtcccag cactaggttc tacaaaatca tagtctttag aacctaaaaa ctcgataagc 32040

atattaagac attctgtagc tatttcaggt tgcgtgtaat attgatctaa cttattcttc 32100

ttgctcacaa atccaccctt cctttatcat caactggtaa agtcttccag tcataccaat 32160

acacgagaac gttcttaaca tctttggtgt ttactaatat aactccatca tacttctgtt 32220

tagcatcata caaaatatct acaccatctt tatccctacc acaacctttg gttttagaag 32280

gggctatatc aaaggagtca tctcttgagc accagacttt ccatctacgt tcttggaaat 32340

cctctaaaca aactaaacgt ttatcagaca aatgtaaaac tgacaggtct gggaagatgt 32400

cttctaagat gtaaggtaaa actttagcaa caccactagt gtctttccac aaccttttac 32460

ttacaaaatc ctgaaagtaa tcggacaagt ctagttctat tttcataagc tattcttcac 32520

cactcatacc gttatcccac tgatcatcaa gttgtcgcat actaacagct tgataacgtg 32580

cttgacggta actatgttca tcaccaacgt ctaaaatatt tttaagttca ccaccgtgaa 32640

taaaatcacc aactagaatc tcacctgatg aatataaatc tttgtaaggg taatttggtc 32700

gaatataccc agtgtaaact ttcatctcct ttacttcgta agggcgttta ctattacgaa 32760

caaatacagt ttcactttta taatttcgat gataacgtgt atcttttgtc agcatatcat 32820

aaactgcacc ttctggtcga gcaacttcct gtaactcttt agtttgtcga gaacgacgtg 32880

ttttcttatc tgcagctttc tcatcaaact ctttctttgt ttctccataa taccagtaga 32940

aacctaagat gtataatact aaatcaatat ctacatttcc attttcatcc cgtagaggga 33000

taatttcacc catgtcatca cgaaattcca tatccgcaac tttctcaata aaatcttcac 33060

ttacacttcc gtgtagaggt gctgactgtt ttgccataac ttctcctgtt ggacaacctc 33120

atctgttatt tgaaaggttg tccattatct caaattagtg ctatcaaagc aagaaatcaa 33180

atctcaaaat aagattaaat ttagatattt gttctatgtt acaagtaaca ttttattcat 33240

cgtccggaag gttttcttca accattttca acagtgttgc ttgattatgt tcaaggaaac 33300

gagtaataac atcttcgctt gcttcgagca ttgttgttcc gagtttctct tggtaagaat 33360

aaatcttttc tacagcagaa acataatctt ccatttcatc ttctgtcata tttaacacgt 33420

aagcattagt gacaacctct gctgccagtt cttgctcttt tgtaagcttg agaaattctt 33480

gtgattgttc tggtgtaagt aagagatttg aacagatacc ttctatttgt gtgtcaatgt 33540

tgttttgcag gatgttaatt agtcgttgtt ttggtgttgt cattatttct cctttagctt 33600

agagcatcta tcgtgaaacg ataagcactt cgtcagtttt acctttacct tttcggaaac 33660

tactattgtt gtagctcatg tttaaatgat gcacttcaaa tacatcttta tgttcttcac 33720

accaatcaat caactcttga tgaactttac cacgattctc aaacacattg gagataacaa 33780

cttttgcacc acgtttttgt aattccacac aatattcaag aagtgttgta ttgtcattat 33840

cgtcccaact attctcgtta taagttgctg tggatgttcc acctccatag ggagggtcaa 33900

gatatacagt agttccagac aaattcccac tctccatcaa tcgttctatt gcatcagcga 33960

agtcttcatt ccacaactca gctttctgaa ttaagtctcg atgagtcatc aaacggtctg 34020

tatcacaacg atgtcgttta ccaaatggaa cattgaatcc tttactactg aatcgcatca 34080

tattactgtt tgaccgacat tgaagattat ataacattgc gtaatctttg caaccacctt 34140

tgttataatc ttcacgaagt tttaagtagc cgtctaagtt atgttcgtaa tcagagttaa 34200

tttgttcaac acgttcaatt tcttctggtg tgagagtttt aacccattta tgtaaaccgt 34260

gtaagaacca agctttctca ttacagatga tgttgtcgaa aagctctgtg ctattaatgg 34320

caatggtcga acttccagaa aacaaatcca gtaatacttt acgattacca tcttggagat 34380

gaggttttat tttcttaaac aattttctct tgtttcctgc aaggtaaatg gaggattgtg 34440

ctgtcattaa tatctcctct ttaaatttac acatccttgt gtaataaatt agtttaccac 34500

ttagtatgcc catctaataa ctggcgatgt tgaatccaac cttttagatt acctgaccag 34560

taattacctt cacggtctgc atgggttaca ccatcttctt cagtatctaa ccaagttgaa 34620

gggtcacaat attcctgcca ttttgtgact accataggtg aagctacatg ctcgaatggg 34680

gaggcgtgga ttttactacc accaacacct agtcgatcat aaaccttcat agctttatca 34740

aacgaattat caatagatct gtaagaaact tgtgcacaac atgaagatga gatggcaata 34800

gcatccttaa cattaacact aacaccatca accttatagg ttagttcttc agggtaacgt 34860

ttatgttcta catatggtgt atgccattca ccagctttaa gttcttcagg attactttca 34920

tcgtaagcgg acttcataca ctcagcaagt tctttgattt cgggttgagc atcttcatca 34980

catcgtaacc aaaagaagtt attatactct gttgctgtaa caacaacctt catcatttga 35040

aacggttcaa gtaaacggtt tacgatttgc ttatgtagtc cttgatgttt taagacttcg 35100

gctaacattg tgacactttg agcacaacca ttccataaat tttcacaaag tttgacatct 35160

tcatgttctg tgtcagcctg cataccagat ttattaatac cccagtggac tggttgtgct 35220

ggactcttac ggacttgctc taacatctta tcgattggaa cggcacgact actcattgca 35280

ttacgagaga ataaacggtg tgtcattaac tctgagtgaa tgaatcgagg gtattctagt 35340

tcaaaagtag tgatacgatt atcaccatta atagagtctg caacaatctt agctttgata 35400

ttaccttcac cgtgtttttc tactttatac aattcttctc tccttactta attcgacgat 35460

taatttcgtt agtaatttta ttcttctgag aaccttgtgc tcgtttcaaa gcttcacgta 35520

gttgttcaag cgacattgat ttcacattaa ctttattctt ttcttttgcc atatttgtta 35580

ttctccttct tgttccaaca tattcaacgc aatcattaaa tcttcacacg ccaacgccat 35640

attcatgaca ctacgtgagt ctcgccaaac atcgtctgac ttggtaggga tattaccttc 35700

ttcaaaatgt ttttgcaagc actttgttag gaattgtttt gtattattgt ccataatcat 35760

agcctttagc aatttggatg actttagttt ctactacagt attctgtcct acaccatcac 35820

acggttcatg gatgtttaca atctgtgcag gaatcgcatg aaaacccata atacctgaat 35880

attcaatagc atcaaaagca accccgaacg cagctccatt tacttgaaga ttaccattat 35940

catagttaca ataatggtgc atatccccca ccactaaacg atgaacgtat tcctgtaagt 36000

tattagcccg tttgtttcgt aaagtcatca aagctgattc tgaactacct ttacaattgt 36060

aaccgtgctc agcaacagtc agtttcccgt aaatacgata agttgcaaag tgaccttctg 36120

gtaggttaaa ttctatgtga tcaaaccctg caatcttaca catattctcc catgttttat 36180

ataatgtgta gtccatccca taacgtccag ctttgaacat atccatacct ttatgttcac 36240

tagcaccatg attaccgcaa acacccacaa attctacagg tatccctaat gaaaagatag 36300

gtgctaagat attaaaccat acatcttgaa tacagttagc catttgttct gcattactag 36360

tatctgttga tgctgttgac tgaacaccgt gtttaagtgc atcctcaaaa ttatctccga 36420

gagcagagaa aataatcttc tcaggttttt cttgtttgat aatatttaca agttcttgac 36480

cataataagc actagatcgt gaagcaatct cactgttcca tgattcactg actttacccc 36540

actgccaatc tgaccataga atctccactg tccttttctt tggctcagac ttgaaaaagt 36600

caatttcgtg aaaaaccaga gacttagttt ctaatttaca ggagttgatt ttatcagcaa 36660

gtaaacccat tacactctct aggtctacat ccttctcttg acggtcaaac tcttcccgct 36720

ggatcttcct gagctgcgta ttgctgcgct gggcgctacg taatctctta gccaaattac 36780

ttacgttcca atcaggatgt tcacataaac cttctaatgt ttgttcagga acaacccaat 36840

cttcttcagt attcacaaca ccattaagtg ttacaccaaa ataattacca tgtttcttat 36900

acacacgttt aatattacga actgtagatt ctttagattt cttacctaag acttctacag 36960

ctacttgtgt attaccttta ttatcttcaa acaaaccttt taggattgct aagattttct 37020

catctgataa acgtttcttt gacaaactat ttctccttac caaacatctt gcattccaat 37080

caactcagct ttaatacctg tcaattggta gaacttgtgt gctttatctt ttacatcttt 37140

aatccaagta tcaaactctg gagataaggg gtctacatca tttactgtga aacctaaaac 37200

ttgatcatca cttggtgaat cgtaatgata agaatatgtt gatagacctt gggactcata 37260

ccattcatag aattcttcgt aatagttacc ttctgtttcg taaagttcac aatcattatc 37320

ttcaaaagtt actgcagatt ccacttctga acaatttgca ccaacaatca ttccacctga 37380

aatatcaata cccatcattt ctcctcattc atcaactgtg ttttataagc ttcctttagc 37440

tgccacaatg tttctgcagc tttagttaca ctcttagctc ggaagatctt tccatacaaa 37500

ccaacatagt ttcctgtatc attgtctttg atataaatac atccatccaa cttgttacgt 37560

tccgtaatgg aaaacttagc gtttgcaata tcttcatgat tttccaaatt ctctctccta 37620

aaattcacaa tgttttaatt cacgaacaat agatgtgatt gattcagcat aactcttatt 37680

agggatatta cctccattat acatcccaac acctttgaac caatcacctt ttgcacgttt 37740

ctgccaataa acaatatctt taacaccctc ttgaatactg tattctggtg aagttatcat 37800

attctcaacc cacataccag cttcgaaagg tgacatattc tttgttttcc aacgagctac 37860

tgtgtgcaat acgccaaaac tttggtcatt aatgttacct ctgttataac gaacattgta 37920

taaaccaagt ttactttctt tccaagcaat agctactgtt gtcaaaccta agtaatactc 37980

ttctccatgt cggtaagcaa tctccatgat atctttctga taaggtgtca attgttgttt 38040

gaatccacat tctccagcat caacatcctt catatacaga gatagaataa tacaaaagat 38100

gagtaagaat aagctttcaa ctccacgagt ttctttaaat tctttgagtt tttgcttaac 38160

tcttactctt ttcttcgttg gctgtttcat gagattttac gctccacaac ctgtttaccg 38220

caatccttgc atgtttgaat gtggaaatct tcactttgtg tgtaatgcca aatctcatgc 38280

ttacaacgaa gtcgtttaat aaacatctta acttggtaaa taaaaagatt aatatcgaac 38340

ataatactat ctcctttgcc gaaggtaaac acttcacttc agtgaaacct ccttggttaa 38400

tgttgatgta atcttagcac atccctgtgt gaagtcaaca atttatttaa attattttca 38460

atctaacaaa cctaactctt catgagataa actttgcacg taatcaatct ccgttttatc 38520

cacatcaata gcttggagaa tatcataaat tgacagataa cgtcctgtat ctttgtcata 38580

caaatcttca tctggcatgg catcatacac ttcttgagca gatagaagtt tgatcgattg 38640

tagacggttg atgttagtca ttgttgttct cctgtgtaag aatttctacc acttgtttac 38700

gtcgaagttt tgcattgctt tctggtgtaa agcctttgga tgcaatccaa gatttttcat 38760

ctcctttgca gatttcaatc gctttcttct caataactgc ctcttcaaaa gaaatgccct 38820

tggcctcgga ataggactta attttgtgac agggtttgca gacgagttgg aagtttacac 38880

ttgtttgtcc tccacagtgc cataagaagg attctgcggt ttccttagat gtacatcctt 38940

ctgattctat cacatgatca cattctagtt tactcccagc cattacggtt ttacagaaaa 39000

cacattcccc tacatttttt gttgagggat gaaactttcc agattgcttt tcttctaaag 39060

ttagtggtcg aagtgaattt ttcttccaaa ccttcctaag tgggtaatcc gaccaaagtt 39120

tacgtaaagc ccctcttaac cagttccaat attgcgcctc cgttttccaa acaactttac 39180

cttcttcact ttcccaagga ttgttcatta taacaacccc gcatctttta atgacaacat 39240

ttcttctaca gacatcatag attttagttt ggtaatttta tctaatggat ctggtgcaaa 39300

ctctaacact tcaggaactt ctttgtattg taagttgtgc catgtagata tgattaggtt 39360

atccatatcg taaatagttc ctgtagcttc gtcatatttt agaataactt ctttcattaa 39420

aaatgttcct catatttatc ccaaaactca tccgattgta attggttact cagagaaaag 39480

atttcagttt gccaagttcc tgtctgtgtg tcaagattta ccattaaatg ttcatcatct 39540

ttgatataac cttctgggac ataacattga taacctacta agtattcttt cttatcattg 39600

ttccacgata cccaactgat tgtatggtct tccgtgaaag accaatgaac acaatcggag 39660

attgagagta gactagggta gctctcagga agactgtcat ggaacttctg aagtgaatct 39720

aaatccatat ttatttctcc ttatttaaaa ttcccacaag cacactcacc ttcacttctc 39780

gcttgacagt aacaatcttc attttcataa acatcagtga ttgtgtagtg tttatctttg 39840

tttaagtgat caagagtttt attacggtct tcctctgata attcaataaa tgttccataa 39900

tccatataat caccatcttg gtaaatgtag tctatacaat aattgatcat ttatttctcc 39960

aaattcttct catgctggac atactcgtaa tgacattcaa aatcaatacc ttcaagctta 40020

gatttgattt tatcaaacac ttcattcttc tcaagagatt tgagctgttt ggcacctttt 40080

aagtcttcgc tcaatataga ttgtatgagc gatgatagct cttttaggtg atcttcattg 40140

ccgcaatagg tctttactgg cggtgacaac aagttgttgt tttcaatctt acttgtatca 40200

accttcatgg ctttgcatag tttgtagaaa tccatatcat cttcataact acgggtcatg 40260

taagcaactt taaaataggt atccatgtat gtcatagtat caacatcaag gttttcattt 40320

ttatggttaa catattgaac accataaggt agaagttcag aaaatttatc tataacagct 40380

tgtaagactt ccttagctgt cttacaaggt ttgagtaatc tgtaagctga ttcatcaccg 40440

aaattaagat tttgcccaag atgcgaaaga caattgtaat tatcggcacc atctccagaa 40500

cacattgctt gccacagaag tcccttaaag ccatagaatt tataatcaac tgaacttgtt 40560

ttctggatta tccctatatc accacaatcc tttgttgaat ctttaatcag tattgcatta 40620

gggaatttaa acttacctct taaagggtta ttctctccaa cgtaagtatc aggatccact 40680

agtaatttgg ggttattttt agcatctttg tctgaagcaa taacaatatt atcgaatttg 40740

ccacatttta gataagataa gtagcctatc caaccataat gttctacacg atcatctgtc 40800

tcataaccct cacgggcaac ttcacaatca agttctttct caacccactc gcgaatctct 40860

ttaagtataa ttgggcggga actttcatta cggttaccct tgtaaggtct tactaaatct 40920

agttcgtgac ggaaacattg cccagatcca atgtgcacct tgatttttgg tatcctaaac 40980

tgttgacgga tctgtttaag tttcatataa atttgaactt tagcttgttc cacagccttg 41040

ctatgttcca tcttaagtct tgcagtttca gttatttcga aatcttcaag attccaagga 41100

tctttaccct caagctctcg ttcaatgttc ttcataccta accaactttt aggttttatg 41160

cctgcaccta aacctttaaa cgtagattta ttcttaaatt cctcttccca cccctctgtt 41220

ttatgcttga caataataaa cttattttcc atgttagcac aagccttcca cactggagta 41280

tcgaaatcgt aatgacctat acaatcttca gtgataatat cacgtatatc cttcacttga 41340

atttctggga atagtttatt atcactttca tctcggataa attctttctt ttcttgttca 41400

ggttcttcca caacattact agatgatgta aaacctttat tttcaaatct cattatttct 41460

cctgttgaag tcttattttt gatagtatgg atttagaata accatcctct gcactctcta 41520

agaacagtaa tccatcttcg aatgacctgt aggttttctc tcttggtttc actgttctaa 41580

aatctactcc ttctgcgata agagcttctt ctgagttacc atacttatta accaagtgac 41640

aacgagttcc atagctatat cccagtttat cccataaatc ttttgcggag tatacatctc 41700

ctttatactc aaattgccac atacgatcct tgaattgtac agacatcaat ttttcaatat 41760

cttcttccca ccctaaatga acaacacgat cccatatcag tcccttttca agacctaata 41820

agtggttcca ttcttctatg gttaaagtaa ttccgtaacc ccttatagta tgattgaatc 41880

tcgtgttgtt tacttgagtc ttcctattca cccatgaaca attactaggt tcataatctc 41940

cattggtatc caacctctcc agttctaagc ctattccgaa agatgattcc atatcaccaa 42000

gaaaattcaa aaacccgttt ttggtagttc ttctccaaga atcacaaaca gctatacctc 42060

gtcctccata gtgtttataa tctttccttg ttgaggtata acatcttcga ttcatatctg 42120

tccatacact atacagaggg tggtcagtga ccctttttac aggaaacttt actccacagg 42180

atatccctcg actacaacca caacttgtag aatctcctct ccataaagaa tctacacgga 42240

tattcttcgt ctctccgcac gaacatttta ctgtcacata tctataagcg tttctgccgc 42300

gctccttgta atagaaaggt gcaatcacgg ttaactctcc gtacacttta ccaacagagg 42360

atagcagttt ttctgtgaac ttactcatca ctcacctcca actctctcat tacctcaacc 42420

aaccactccc tgataacttc gttatccatc acaagaaatg tcccttcttg gtttgtcacc 42480

aaatggttga tcacttcatc aatcttgaga gtaacctcac cactaacctt attgtgttta 42540

atcatacagt ctccttttac acaagtatga aattacattg tctcaaaatc aatgaagttt 42600

tggaaagaat taaaagttat atgatataac taattcttag gtggatgata ccaagatgtc 42660

tctacaccat cgaaggtttt gaacttgaca ctagctacag ctttaccttc ttcaatattt 42720

tcatcaaaca gtccaaacat tactttttca atctgaacat taccgtaagt ttgaatatca 42780

caaaccttct taacttcttc gaagtctaac catagattag gttcaatatc taacattaat 42840

cctccttcaa agaatcatta actgattgca ttaaactgtc aattaactgc tccaatgtcc 42900

acccatcatc aagaaggctt tggtaaagtt cgatatctgt cccaagataa tatttaattt 42960

tctcattaaa ccaattacct tcttgacctt ttacaaagta gtgtttcata tcacctccta 43020

aataataaac agcatccaag aatactatat ctattcttac gattcaagga tgctttatta 43080

attattttac ttgtttacgt gccaaatcta cataatcaag aatattctct aacgtcttaa 43140

cttcttcaga caccttacct tcagtcattt cagccaccat cttcttgatg aacttggaat 43200

aatccccctt cttagcttct tccgagattt tagttttgta aacttcgtaa ccttgatcaa 43260

gggacgcctt aattgcatct tgtgcagatt ggatttccaa tgtcaaatcg tagacacgtt 43320

ggagggtgtt aaccattgtg cttgcatctt gctctgggtt tacgaattta atttgtactg 43380

tttcttgttc gatcataatt atttctccta ttaatgtctt tacttattga actgactatc 43440

ttgtgtaaat ttatatagtg atactacttt catgctttct ccttatttct tatctataag 43500

tgcataacct aaaaccaggc taataaatcc tgagaccatt tgaattaacc agtaaaatcc 43560

attactaaac aatactgtaa agaatccttg gtcggttgta aatagttcat aaataccgtg 43620

accaatccaa actacgttac ccgaaacaag aagtaaccac cccaaaagca taaaaattgc 43680

tacaaacata actttctcct taataaataa acttctctag acctacgggc tcatgaaata 43740

aacttgattt tatgatcttc ggcttatcaa atactacacc actttctaaa tctttaccag 43800

cagtaaatac gtaataatta cctactttct tatagcttac atctccgtaa cgaccttttg 43860

attcaataaa acctacttcc ccatccaaat caaatccgtg gtatagctca tcttcatgaa 43920

cgtatttact ataattacta tcagatatta cacggaaaac tccttctaca tccataattt 43980

ctgacatctt aacacataat gcgaaaagat gttcatgtag ttcataataa tcttccacat 44040

taataaggga tgaacttaac aaactgatca cttcttccgt ggtgaaaagg ttcttccatt 44100

ctttaaggtt tacttgaaaa tcttctggac tgtctgtcaa ttcacaaagc atccctactg 44160

taaagagtag gtcccctaca ccatcggctt gcatcacttt atcaccagta aaccaacctt 44220

gcaagaactc attttgacca aacatttctt cacgacttag ttgtgtgtag acagggataa 44280

gtgatttatc ttctacttca ttaccaccaa ttttattcca ttgcacaaca tcttgaataa 44340

tagcatcgaa tgtattacgt gtaaattctt tcttctccaa cctacttctc ctctaacata 44400

actaattcat tttcaaaata atcttcaaac ttactgacaa gctccttgtc caatccttca 44460

tcattaagtt tccaaatgat ttcatccttc atacactcta cttgttgttt ccatatatca 44520

acaagaatat atttctggat atgtcgatca cataagtaat aaatagtaga ttcatttgta 44580

gcatcacaat tatcgctgtc ccacacaaat ttaccaaaag ctttcacgtg gtgaacatgg 44640

tcttgccaaa cgtactctac tggaaggtaa tgttctgttc catactggtc attaatcttg 44700

tctacaatcc gtgtaaacat gtcgtggttt agcataaact gttttcctct ttcttacgat 44760

aatattcatt ttgacgttta acactttcta cgaaacgtaa tacatcattg taattgacag 44820

cttcaactcc catagaaccg tgataagagt attcgtcatg ttcttctgct gtatagattt 44880

caagagaatc ttcatgtaag cctaaactaa aacaggttac actaaaatct aatttttcac 44940

aaactttata taagtcttga ataagacaaa gcatatcaca tctcctctac ctgatctagt 45000

ccaacccaaa ctctattttt gttcaaatct aaaactactt ccaaccaagg gtatccttca 45060

cttgtgtcaa ctttacctgt tacaccagtc ataccttcat ataagcctgt taaaatttta 45120

actttcatag tattcctcca cccaaccttc ttcgatatct atgtaatcaa accacatatc 45180

ctgtccagcc aagtccttac caaacataaa cttctgagag ctcacacaga aatctgcagt 45240

aacaacttga ccatttttaa cattagaagg tttgttccag atacgttcaa tattattaat 45300

taaaaattca cctttcatag cacctcctct agatatttaa caaaaacttc ttcaacatca 45360

aaccaagttt tacacactgt atgtggtttg tcaagctcaa cttcttgaac ataaggcgat 45420

tcccacttaa taagtttcac atcatcagac atttcccgta gaaagatatt tctatcatca 45480

atcaacaaat cacatttaat aaggtgcttc tttctggcag ggatgaaatt gaaatcttct 45540

ggtaacatga aatcaaattc ttccttcaat cgtttaagtt tgtactttat ctgatcaggg 45600

cagttcatgc aataggaaat aaaaattatt tcacaattag cttcatagag gttacgaatg 45660

acgtcttttg cgccttcgtg aatatcacaa tttttataag tactcggatc gctccaatac 45720

ttaaaaccat cttctgatga aataccttca aagtacttag ttagattata ctctacttta 45780

ctgttgtttg aatcaccaat atactgttta tggttaaaat ctttatttgg tttaagtatc 45840

aaccacttaa gccggtgaat gtctgtgtta aataatgtta tgtcaagatc tataccaatt 45900

ttaattttct gcattcaacc acctattaac cagaaactca acatcattca agaaaccttc 45960

ttctgttccg ttattcacta tttgataaaa ttgaacttta tcatcgaaca tttctggtgt 46020

caaataatct cgactatctc cttcgaaaga acaaccttca cgttgaatac gtatgactaa 46080

aacattttct gcaccaatat gatcgataac tggttgtagt tcttctggga agccagaatc 46140

tgatacaaac acaatacctt cttttggaag tgaattcaca aaagctttac caaaagcatc 46200

tttaccaaat gaaggtttga tcacattttc actcatatgt atcaggaatt ctcgttttga 46260

atactgcttt cctcgaatac gctgggagat agtccccgta taatctttat accacatatc 46320

tggatcttcg taaaataaga gttccttacc tttacgatta taaacttctc ccagtgtctc 46380

atcataacca tctttgaagg tgtttacaga gacacctagt gtgtttacac agatcttgaa 46440

tagttcatct ttgaatgctc gatgataact attagtcctg atcttacaca tatgagatga 46500

tgcagaatct ttaccacttc tggcgggacc attgaatact accaccttta acatattaat 46560

cctcctcttt aattttacct aacaaaatct tcgctgtatg agccttagat ggtgttgtat 46620

attcacccat acgaacgtat tgctcaagca ctttagcagc attagaatag cttgtgaagc 46680

cattctcaca agtttccaag ggtgttttcc ctttaagctt acctttcttg taaggcgttg 46740

tgtagtgttg cacaagttca tatgctttgt caaaggttag atttgtgttt aaattaatca 46800

tagtagttcc ttattaatat ccgtgattgt ttgctgccat atcttttaca gtgatgtaag 46860

ttagtgagta atcagatggg ttgtagtaaa gcaagtcatc aatctcggct tgagattcgt 46920

tgataacacc tttcttcatg atgtgaatct ccgattgaca accgttgtca ctattaaaca 46980

tttcatgtac aagaatttca atagttttag gtttcttttt aagacacctt tgtagtgata 47040

acacccactt acgttcttct tcagtcaact tattcatctt taattttctc cttaacaaaa 47100

tcaactaacc ataaatgtaa aggtttatca atatcaacat ctagataacc aagttctttt 47160

gcgataaaca aaagagattc atggtcttgc ttataataca gtaaatcttc aaccagcaca 47220

ctcacaggta actttgacaa ttcttctttt attgttggtt tcatagtttc tcccaccaat 47280

tagttttaat cttacaatta cccatctcaa ctccacccca tccatcggag cattcccaac 47340

acacaatcat ctcatctttg ttgatctgct tgaatgagtg taccacgccc ttcaacattg 47400

tggtaggtgt ttttactact acactcgttg ccattttcaa caagagaacg cataagtgct 47460

tcacttaaga agtctgattc aaccttaacc catttactca tagtccctcc aattgttcta 47520

caattatccg tagtgaaggc cgtaaacttg ctgactctcg tgcattggcc caagacgtta 47580

acgtatccaa gatgcagatg aatactgcta cacgtttacg tgatgcactt gaaactgccg 47640

gtatcaatat tcaagaagtg ctggttcaag atattacatt tgatcctcgt attgctaagc 47700

aaattctaga tactcaaaaa cgtatccaga acgaagaaca aaagaaatct gatgagcgta 47760

tcgctaaaac tgaagcagct attaagaaag aacaagctat cggtgaaggt aacagaaagc 47820

gtgaagcagc agatgctgaa gcttaccgtg aaaagacaga agcagcaggc cgtaaagaag 47880

ccgctatttc tcgtgcgcaa ggtaaagcag agtctattac tctacaagct aaagcagaag 47940

cagatgctat taaactaatg gctgaagcaa accgtgactt ggctagatca atcacacctg 48000

cactacttga atacaaacga atcgagaatg aagcaattct ttactcgaaa tctcgtggtg 48060

aagtaccaac aactattatt ggtgatactg atctacgtgc aatcggtgtt cctgtagcaa 48120

ccggcactaa ataattgaac cagtccctgc caaatggtgg ggactctttc tttggattat 48180

tcgataaggg aggacttcgt cctcttttat gaaataacct caagaaaact actaaaggag 48240

tgaaccatgt ttcacaattt ccaagaagct gtaatgaaac aaatcgaaaa gatgtctcgc 48300

tttccactat ttgaagtgga ttactctcgt gaggagatct ttgaaaagta ccttaactct 48360

ttccgtccag aagataaccc aatcttccgt gagcgtactg aacatgattg ttcatgctgt 48420

aaatcgttca tccgtcaaat gggtggtatg gtcgctatcg acaaagataa agtgatcaca 48480

atctgggacg ttaaaggtct tgaaccaaaa taccaaatcg ttgctgatgc aatggctctg 48540

tatatccgtg ccctgcctat caaacacgtg ttcaaacgtg ctgaagcaaa aattggcaca 48600

aagcaaaacg tagaggtaac tgaaaatggt tcaattactt gggatcattt ctattctgaa 48660

gtacctatgt ctgcccgttc tcgtgatggt gatcgcttgc gtggcgaatc cgcttctcat 48720

aaagatagtc tggaacgttc tattacagaa ctaaacccag atcatgtgga agaattggtt 48780

cagttgtgta aagacaacaa cctgtactta ggtagccaac aacttcctaa actacagaaa 48840

cttcaatctc tgcaacgcga atacaatgca gctaagaaca aggagctatt cctatgggta 48900

cgctctgtgg agctgggcgc tgcgtctact attcgtaact ctgttatcgg tcaggttcta 48960

gtgaacatgg ctaaaggtga cagcctagag actgctgtag gtaaatatga ggctatgaca 49020

gccccatcta actacaaacg ctctaaagct atcgtatcgg aaggtatgat caagaaagct 49080

caccagacag tcgtacagct tggtctggaa gactctctac ttcgtcgtca tgctactaaa 49140

gacgacatca caatcaataa cgtactttac gctgataact ctatccgtga atcgcttggt 49200

gcattcgata tgttggttgg ttcaacgagc cagtctacag aaatcaaagg cactccaacg 49260

cctatgggta ttgagcagtt cttagaagaa gtactaccta actcaagcaa gcttgaagtc 49320

tacatggaga acagccactc agctaaccta atgagcctag tagctccggt taacgctgat 49380

gcgcctaaca tccttaaatg ggacaacaac ttctcttgga cttacaacgg cgaagtaacc 49440

gactctatct cagagcgtgt taaacgtgcc ggtggtaagg taacaggtaa attgcgtgtg 49500

tctctggcat ggcataacag tgatgacttg gacttaggtc taactgaacc aacaggcgaa 49560

catatttact tcggtcactc acgctcacgt caaggcggtc gtcttgatgt agacatgaat 49620

gccggtggac gtatgaaccc tgtcgatcca gtggaaaaca ttatctacgc agactctgtg 49680

aaagatggtg tctaccaagt caaagtgaac caattctcta aacgcaacaa taacaacgac 49740

aactgtggtt acacaattga agttgagtac gaaggtaaga cctacacgtt tgagtctgcg 49800

gctcaaatgc accaaggtca gacagaccta gtgactgagt ttgaagttaa gggtaacgag 49860

atttctttct ctggttcacc aagctctgct tcgaaagaag tatggggcgt cgctactaag 49920

cagttcacta ctgttaaagc agtatcacta tcacctaact tttgggatga gaaccaaagc 49980

ggtaacaagc actatttctt cttcttagat aattgtgtta acccagaatc agctcgtggc 50040

ttctacaacg aattcctacg tgatgacttg tacgatcacc gtaaagtatt tgaacatctt 50100

ggttcaaaac tgaaagctga atactccaac gatcagatgt ctggtcttgg cttctcatca 50160

accaagcgca acaaacttgt aatgcgcgtc gataaccgtt tatacgaggt acagttctaa 50220

tggaactatt cgcaaaagca acaaaacaac gtttacgttt taaaattggc aaaggtacta 50280

tctctacaga agacctttgg ttcattggca tggtcgagct agataaacta gccgttgctc 50340

tacacaaaga gttggaatct caggaaactg gttcattcct aaatcctaag aagactgacg 50400

ctaaaactca attagagttt gatgtagtta agtacgtact agaccaacgt ctagaaggtg 50460

ctcagcgtgc tgagaaagca gccgttactc gtgctaagaa cgaacgcatc gacaagatca 50520

tccagcgtaa acgcgatgaa gaacttgaaa acatgtctgt tgaagagttg gaaaaactca 50580

aagcagaata atccctaacg agagaggcct acggcctctt ttatgatttg aaccaaaagg 50640

aaactacaat gtttggtcta tttaagaaaa aagctgctgc tgcagcagtc actctacata 50700

aagtagaaaa caaagatcta atggaagcta tcgtagctgg tgctgtactg gttgctaacg 50760

ctgacggaga agcttcggct gaagaactaa ccaagctatc aggcatcatt gagaacaatg 50820

acaacttgaa gcactttggt tcagctatcg gtcagaccat cgacaagtac atgggtatgt 50880

acgagtctgg tcgtcgccta gctaagtcta agctgatgaa agaaatcgct gatgttcagg 50940

cagatgaaga tcagaaagtt gaagcattca tcattgctat cgagatcgct gatgcagacg 51000

gtgagattga tgatgcagag atggctgttc tgcgcgagat tggtaaagtc ttgggcctta 51060

acccagacaa ctacatctaa tgcaggagcg tttactcgcc ctattccttg gactgctatg 51120

tttactagta gtcatcgtag atttcacgag caagatcctg tctatcacgg tagacggggt 51180

tctcgtttgc ggaatcgtcc tcttgatctt tcgtctgcgt aaacttgatc aagaaccaaa 51240

ataatccaat aattcaaagg agagactcat gtctcaagaa caagcaatca cagtaacatc 51300

aaaagcaaca gcatctctag acaaagtggc aacaagcgta gcgaaagcag tagctgacct 51360

acaatctgta actgaagtac acggtgacct tctaggtcaa gtggctgaag cgcaacagaa 51420

cttgaaccaa atcgaagaag atactgcaac taaagtacgt aaagcaaaag ttgaacttga 51480

ccttcgtgta actgagcacg ctgatggtgt tctacagaac cttctagctg agcgtggcct 51540

aacttctatc gacgaagatg cgctagttga gctacgtcat aaagctgatc gcacttcata 51600

cgaagttgat gttgaaatca agaaagcagt taatgcagca cttgcgaaga aagacactga 51660

gcacaaactt gaacaagcag aaactaacca agttaatgca gtacgtgaag ctcaaatgga 51720

atctcgtatc gagtctctag agcaacaact tgaagcatct cgtcgtgaag ttagcaacct 51780

aactgaacaa ctatcagcag aacgtgaagc tggtacagag cgtgcaaaag cagcaggttc 51840

aatgactatc aatgccggtg gtaaataata gtcgccatgg tagggggagc tacgctccct 51900

tttccgaacc ggagactcgg atgagattcg gtatataaaa cggggttgaa ccaacctagg 51960

gcagatgcaa aaggctttgt caggccagct tacatcgttc agtcggagga ctgaattgaa 52020

ccaataaact aagcgaaggg ggacgccaac cccacgacac taactactat tcgatattct 52080

ttctttatgg gacattccag tggcttaaat gcagcatgga attgacaggg catagctagt 52140

cgtagagggc tgatcacctc gtagacacat taccccgacc gtgtagcaat actagttcaa 52200

gcagacgata aaaacagaga gagaataatg aatagccgtt gacatggata ttctgaaata 52260

tgttactcat attttattcc ttatgaagca gcacttcctt gtgctcggca agtacatgct 52320

agaaattggt gtacagggta ggtgagatgc ctaccgtcat tctgaatgtg ttttgaacca 52380

gaatacatcc agaatggcat tgctgttcag tgacattttc taatgtctcc aaagttggaa 52440

tatatctatt tacctcatag tagatcggcg agagtcgcta gaaactggac ttcgattcag 52500

gtgggatgcc tgatacctat tcactacaac acgtcctgat agtctgtcgt tgtagcctgc 52560

cttcgggcag tcggcaagcc gatgctagaa attgatcgga tgagtgagtg agagacttgc 52620

acctaattta actactggaa tttgaaccat gaaaaaagta acccctgaag tggctgaatc 52680

attgaaagct attactggtt gcccaagaag aactgaagac atcgttaaaa acttcgacat 52740

tgatcgtatt tcatttttga atcatattcg atctgctcgt gcttcgggta ttccagtaga 52800

acatattggg ccaatgtccc atcgttattt ctatctccca gccgaatacc gctataacat 52860

caaagaaaca gttgcagcat caattaaaaa gatcatggcg aatgctcggg aagaggatga 52920

cataactgtg gaatacctcg cacaagtatt ggaagtaacc actaatcatg tacgttcagt 52980

attctatgcg tggaagaaga aaggccaagt aatttacagt gcacctactg gtgttggtaa 53040

acaaaagaaa tactggacag tgcctaccga acctgttgaa cgagagtctt gactctggtt 53100

catcgcaaac tctgcgatta ataagtgatg agtgaagtgc actgagagag tgcacaactt 53160

taattaactt agaggaatag gcatggcaca agatacctta gctaaaaaga agcaagcgta 53220

tatcctgttc aaacaaggtt ggactgtgaa gcaagtagcg cagcatatga acgtaagtac 53280

tcgtatggtt taccgctaca aagacgaata tgaattacat actctccgtg agttccgtga 53340

tcaattcaaa gttgctctgg aaatcgcaga caaacaacaa cctttatctg tatttttgca 53400

gatcttaaaa gcacaagcta ataaggaaaa gaacagtgaa aaatccgctt aaatttaaaa 53460

cagtagatga tgtaatgtcg gatactgtcg gtcaactaca agagatcgaa gaacgccaac 53520

agcgaaagtc tgaagcgtta gaaaagcaaa ttgaaaagtt gaaccaacag aaggaaactt 53580

ctgacggtga agttagtaag gccaaaatgg gcattcgaaa ctttaccaaa ctatttactc 53640

aggaattgcc cgacaacgaa gagtaatgac aaaacctaag tctcctgttt aaggggactt 53700

tatttttgtt attgctaacc gcacctctat gctgaaggtg gaaaggttac agttggctgg 53760

gcaactgaga catgcaagtg aaccacctta gtagttttct tttacttgcg tgaccccagc 53820

attattcccg aataaggcgg cttatccttt aatcgtatgc taacacgata tatgccgctg 53880

aagtccccgt ccaagagtag taatcttgcg gggcaaccta tttacacaaa cccccctatg 53940

atctgaatac gccgggttaa tttgttttgc cactgcgtta actaacgaac acctaaacat 54000

gaaaactgag gtgatcgtct atggtcttta ttcagtaggg gttttttatt attgaggcag 54060

gaacatgtct aaagaacttg aattcgattt atttcgtacc tccaaggaaa cacaatttcc 54120

tgctttagta gatttgcagg tactagaccc taattcagca gaaaagctaa tcaacaattg 54180

gttcaatact tcccagtcaa atgaactcct agcgaaagaa atacgtcaaa atcgtatgac 54240

gatgagtcat ttgaaccaag ttattgaaga acagaagcga gctatcaaga aagaaggtat 54300

agctggctta gtgatcggag ccttaatggg tgccgccact gtttctttac tagttatgat 54360

ctttggggga tagaatgcga tcgtttaatc aaaaagagat tgaagacttt ttgaaccaat 54420

ctcctagtaa ggcatacaag ctagtgcctc accaatttaa ttctagtcat gcaggcaaag 54480

cttgctgtac taagtgtggg ctgctcgccc ttcgaaatag agctaccgac tggtgtgttg 54540

aaaaaggatg tgagtttgag aaccaccctg attacaccaa tgcaatgaaa cgtttaacca 54600

aataaccttg agagaacaca atgagtaatt tagattttgc agtaactcca ttgaaagcaa 54660

tcccattgat tgaacgtgca atgcgcaaac gtattgtacc aatcctgaag ggttcaccag 54720

gtatcggtaa atccgatatc atgcgtaaga tcgcacgtaa gcacggtctg aagctagtag 54780

atattcgtct agcacaggtt gacccagtag agcttaatgg cttccctaac ttcactgcgg 54840

acggtaaagc ggtttacgct ccaatggata tcttcccaac taagggacaa gagctaccac 54900

ttctacctga attcgaagac aagaaaggtg aatatgatgc cgtacttggt tttggttcag 54960

aagaagagat caaagaattc cagaaagagt actgttacaa aggttggctt ctattcttcg 55020

atgagattac ttctgctaac cgtcaggtgc aggcagcagc atacaaaatc atcctagacc 55080

acttggtagg ccaattcgat ctacacccta atgcactttg tgtagcagct ggtaacctgt 55140

cttcggataa agctgttgta aacccaatgt ctacagcact tcaatcacgt atgtctcact 55200

tgactctacg tgttgataaa gacgaatggg ttcattgggc aatcgagaac aacgtggatg 55260

ctcgtgttat tgcattcatc gaataccgtg atgatctgct tcacgacttc aatccgaacc 55320

atacctcaga taccttccct tgtcctcgta catggaaatt tatgtctgac cttgtggaag 55380

acgatgaagc aatctctagt tcactaatgc cactattggt tggtactgtg ggcggtggcg 55440

cagcaggcga gttccgtacc tttacagaaa tctggcgaga attgcctagc cttaaagata 55500

tttcagtaag ccctgagcgt actacagtac ctaaagaagg cgctacctgt tacgcaacag 55560

taggtatgtt ggcatcatct gttgacctaa catcaattga accaatctgt aaatacgttc 55620

gtcgtttggg ttcagagttc caactagtgt ttgttcgtat ggcgttcaaa tacaaccgta 55680

ctctaatcca aaacccaaca ttccagcaaa tgttgaaaga cttccgtaag gacttaaaag 55740

acgaggaata aaatgtttga tcgtgataag gctctaagta aagctaaagt tgccattatg 55800

atgaaaccga atactgcatt catttcatct gtattaatgt cacttaaatc taagtgggat 55860

gataaacatg ctacggctta tacagacggc ctaagcatcg gcatttctac tcctttctgg 55920

gaaagactaa caccggggca acgagtattc ttgttgctcc atgaaacttg gcatgtggcg 55980

ttttaccatg taactcgctg caaagaatcc aagaaatatg aaccacagaa atggaattat 56040

gcctgtgact actacatcaa cttgatgttg gtcaaagaag ggtttgaatt catcgaaggt 56100

ggtttattgg actacgccta tgatggtatg tctgtagatg aaatctatga tgcattacct 56160

gagaacctac ctaacaacct gttagatggc gatatggtag aaggtggttc aatggaagaa 56220

gaacagccta cacctgatga cgttaaagca cgtatcactg aaactctatc aagagcaatg 56280

atgcaagcaa acatgtcagg ccaagctggt tcaattcctt tggatattcg acgtgcagtt 56340

gaagagatcc gtaatccacg attaccttgg aatgttgttc ttgagcaata tcttagtgat 56400

cgtatccaag atgaatattc ttggaacaaa cgcaatcgtc gtttctccca cgtgtatctt 56460

ccggcgttag actcagaagg tatgggtgag attcgttgtt atgtggattc ctctggttca 56520

gtgagccaga aagaactatc tcttgaagta aacgaaatgt gttacatcaa agaggtatcg 56580

aatcctacat taatgacact tcgtgccttt gcgtgttctc tgggtaaagt acagcagttt 56640

gaacgagatc aagaaatcga gttcgatgtt gacgtaggtg gtggtacaag tttcggccct 56700

atctatgacg acctgatgga caacccagat actgaagtgg ctgtaatctt tacagacggg 56760

tgcctaaacg tacctgacac atcggccctt ccatgtgacc ttgtatgggt tatcgtgaat 56820

aaccctactt ggacgactaa cacaggccgt gttattcaca tggagataaa acaaggagac 56880

tagaaatggc acgtaatccg cgtaaacgta gtgtgtcttt tcgaaactac gccctgcatc 56940

atactaagtt atgttgcttt tccaaagttg aaccaaagca gctagatctc gtggtgaaac 57000

atggcgcttg ttcatacaaa ggattagact ttgcgactcc ttggcattac ggcgtgattc 57060

caccctgtga tgaagcaaag tgggggcgtg atcttgaaca acttgatcac atctggttca 57120

taactagcga atggaagaag attgaatttc atgtcattca atggttgaac cactgcaata 57180

gtatggtaga catcgttaag ttgttgccag actcaattac cgataagttt gatacaccta 57240

ttactggtga gttaaccctg tcggacgagt ggatagacat ggttcaaaac tctgatgagt 57300

ttaatatgtt agaaacactt gtgttcacta acgagatctt ggagcaatga tgtgccatac 57360

tatttggcct tcatcaaaca gaggtctata tgcgccatct tattcattct gaaaactacg 57420

atatggcagt actcattaaa gatgctgccc ttcgtaactc tgaaattgaa aaacactatt 57480

tgaaccaaaa aggattcaaa gctatctcct tctcacttga ttatggtgga aagaagaagc 57540

cgaccgctac agtggtgaaa gattatcttg atacgttgct tccagtcctt gagcaatgtg 57600

gagtacgaga tctgcttgta tgcgatacgg aatatttcaa aaagctttgt aaggttcaaa 57660

aagctgatcc tcattatggt tacattctgc cagtagctat tcccggctac gagcacatga 57720

atgctgtctt atgtaccaac tatcaagctt tgctattcaa cccgaatatg caagacaaga 57780

tagacctagc tctcggaacc atggaacagc acaaagctgg ttcatacgaa gagattggtt 57840

caaacattat tcagcatgaa gagtacccag atcagtatga tcaaattcag aaatggctcg 57900

atagcctatt acttttgcca gcccttacat gcgatattga ggcttttagt ctcaagcatt 57960

atgacgctgg tattgggagt atttcttttg cttgggacac tagtagcggt attagttttg 58020

ctgttgacta tgtgccaaca ataccccatg aaattaaagt gtggtgtaag aaagacaaga 58080

aatataaatc ccgacaagcg gtaggcaagc agggatacaa tatgcgtatt cgccaaatgc 58140

tcaaagagtt ctttatcaag tatcaaggct ctctgatctg gcacaacgcg tcttacgata 58200

tctcagtgct tatttaccaa ctctggatgg acaacatcat tgatcaagaa ggtatgcttg 58260

aaggacttga agttatgaca cgtaactttg aggacactaa gatcattact tacttggcaa 58320

ccaatagctg tgcaggcaac aagttaggtc tgaaagacca agctcatgag tttgcaggca 58380

actatgccga ggacgacatc aacgatattc gtcttatccc taaagatcag ttgttacgct 58440

acaacctgat tgactgtcta tgtacttggt acgtcatgga caaacactac gacaccatgg 58500

ttctagacga acagtacaat ctgtacgttg acctaatgaa gcctcatttg attgatatca 58560

ttcagatgca gctgaccggt atgtgtttgg atatgaacca agtccataaa gctgagaaag 58620

agttgcaggg tatctacgat actcacttaa atgcgttgat gggccaccca atcatcaaag 58680

agttcactca ccagcttcgc cttgataaga tcgaggcaga caacaagaag ctcaaaagca 58740

aggagcgatc ctatgaagag gtatctcacc ttacctttaa tcctaatagt ggtgtgcagt 58800

tacaacggtt gctctacgag tgcatggagc ttccagaaat tgactacacc aagtcaggtg 58860

ctccagccac cggaggcaag accttaaaga aactctctaa gatgacacag gataaagacg 58920

ctatctttat tttgaaccac ttgagagaat acattggcgt gaataaggta cttacttcgt 58980

tcattcctac atttaaggaa gcacccctag caccagacgg aatgcattac ctatttggtt 59040

cattcattct aggcggtact gtatcgggac gcttatcctc caaaaaccct aaccttcaac 59100

agattccatc tggttcaacc tatgccaaag taattaagaa atgctttgtg gcacctaaag 59160

gttggctatt ctgtggagca gactttaatg ctctagaaga taggattaat actctcttaa 59220

ctcgtgatcc aaacaaagaa aaagttctgc tagatggatt tgacggacac tcgttccgta 59280

cttatcactt ctggcctgaa aagtttcctc acattgatcc tgaagaccct gttagcgtga 59340

acaccattca gaaagactat gatgcagatc gtggtcgatc taaaccggtt cactttgcta 59400

tgcagtacca aggcacttgg gcaacactca tgaagaactg tggctttagt gagcaagaat 59460

ccaaggatat cgaagctaac tatcaagttc tttataagaa gtctttcgat tgggtacatg 59520

cccgaaccaa agaagcttca gacaaaggat acgctactgc tgctttcggc ctacgtatca 59580

gatgcccatt gctaggtaag accatattag gttcaagggc tactttgaac caagcggcag 59640

cagaagcaag aacacttggt aatgctatct caggacaatc ctacggattg ttaaatggtc 59700

gagcatcagt tgaacttatg cagaaagtgc gtaatcatcc taagatgaga acgtgcatta 59760

agcaatgtgc gcatattcat gatgcacagt acttctatgt gaaggatgat ctcgaagcaa 59820

tcgaatggtt gaatcgtgag ctaccgaaag ctatgagatg gcaagaactt ccagaattac 59880

aacatgaccg tattacacta ccggctgaat tagatatctt ctaccctact tgggcagacg 59940

gtttccagct agagaatgat atgactcagg atgaaattgc tgaagtttgt cgagcagagg 60000

ctcaaaaacg aagagagagt gaaaatgggt aaaacctaca aagatcgggc taaccacgcc 60060

ctacatcatg gttatgaacc aaatggtgat aaagagcaag agcttgctga tatcttagac 60120

cgtaatcctc acaagggtct acgccacggc aacaatcgta agagtgaagc caaatctaag 60180

aagatcgaac gccgcaaact tcgtcgccaa aaggagaatt tcaaccatga ttaaaatcaa 60240

taacaagcat caaaatgcta cgttacctca aactgtcgaa aacattgcac gctggcataa 60300

cgagcgtaac ctgatcgacg gaaccacttc gtataagcaa actgaaaagt tgcttgaaga 60360

attcaccgaa ctagtttcag ctcaattggc ccgagacacc ggtaagaaac cttcatctga 60420

attcgtttat gacgaaactc gctcaatgtt agaccgcctt tacgaaggtg gacgaatcaa 60480

aggaattgaa ccagaacatg ctgaagatga acttcaggat gcaattggtg acatgcttgt 60540

ggttcaaact aacattgcag agcgtgaagg tttcactatc tccgatgctg cattcaacgc 60600

ttacctagag atcaaagatc gtaagggtaa gatgattgat ggtacattcg tgaaggaggc 60660

agacctgtga tcaatgtaca aattattgct gactctaaat atggttcaaa tcgtgtaact 60720

acgtttgagc ttgagtatcc gcgatacgtg catagtgagt tgatgactca ccgcgtattc 60780

tcccgtaatg ctgcatcctc tcgtgctatt cccatcgaaa agatgatcga acaggctacc 60840

gagaacacgg ttatcccaat gtggtctaag aaccagaaag gtatgcaagg cgaacttgta 60900

cgtgacctag agactatcaa tgaagcaaac cgtatcttag gtgtgatgct caacgatgtt 60960

gtcgaaggtg tacgagagct aagcgaattg ggtatccata agcagaatgc taaccgctac 61020

cttgaaccat tccagcacat caaaaccatt gttactgcta cggaatttga gaacttcttt 61080

aacttacgtt atcacgaaga tgctcaacca gaaatccgtg cactagctgt cgagatgtat 61140

gctgcgtact gtaagtctaa gcctaagttg ctcaagccga ctgaatggca cctgccttac 61200

gttgatgtac cagtagctta ccctggttca gaatctgata gcagcgatgc tatttcacta 61260

gatgaagcta agaaagtatc agcatcatgc tgtgcccaag ttagctaccg taagaacgat 61320

acttctgttg aaaaggcaga gtttgtctac gatcgtctag taggcggtga accattacat 61380

gcgtctccat ttgaacacgt atgtcgtcct ctagctcaaa gcgaagaaca gcgtggtaac 61440

ctaatcggtt tccgtcagtt acgccagaat attgaggaca aggtatgagc aaatctgtgg 61500

tagatatttt acaactggct caagactgtg tgggtgatcg tgcggcagaa cgagatacag 61560

atgccgaacg tagcatgaag acgtgtattg atgcatttaa tgccatgtat ggcaccgagc 61620

ttaccgaaga acagggatgg atgttcatgg tgttcctgaa ggcttccaga gccaagggag 61680

gtaagttcaa gctggacgac tatgtggata tggcagctta tgcaggttta gcaggagaat 61740

gtcatggctc aaaagaagtt cagtaataat cacggtatca gcctaccctt agcggtatgg 61800

ctggcccgag acgagtatga tcatgatgat agaaccaatg tgatctcagc aactagtttg 61860

ttgaagtcgg ttcgtcagat tgtgctcagt acccgtgtgg tgccaacagc aggtaatatc 61920

gaggatattt ctgataggct tgcttcccgt attggtactg cgttacacaa ctcagtagaa 61980

cacgcatgga cggaaaatta tggacgtgcc cttagtgatc ttggttatcc tcaacatgct 62040

attgatcgtg ttgtggttaa ccctacttcg gttcaaaaga acgatatccc tgtgtatttt 62100

gagcaacgga gtgagaagac ggttggaaaa tggattgtct ctggtcagta cgatctcgtt 62160

tttgacgggg ttgttaatga tgtcaagtct acttctgtat atacgtggat aaacaatacc 62220

aacgacgcta agtataagaa gcaattatct atctaccgat ggcttaaccc tgagattatt 62280

acaaaacctc atggtgcgat ccagttcatc tttaaggact ggaacaaaaa catggcgatg 62340

ggtaaagata aaaaatatcc taaacttcct gtgatggaat acaaactcga actggatcaa 62400

ccatctacca ctgaacagtg ggtgacggac aaattgaacc agatagatga atatttacaa 62460

gtgccggaac ctgagttacc gttgtgtacc ccagaagacc tatggcaatc tgaaccaaag 62520

ttcaagtact attcatcgcc ttcggccaaa cgtgctacca aagtgttcga taacatggcg 62580

gaagcaagac gttacttaat ggttcaaaag aagggaatgg gactagtgaa agaagtatat 62640

ggtgaaccta ctgcttgtaa gttctgtcca tgtgccggta tctgttcaca gttcaaaggg 62700

tttgttaagc aaggtctaat taaataaggg gctatcgccc ctttaatgag tctaacatct 62760

aaggaataac atggaactca aaccattatc cgaggtggac tataatccaa ctcaagagga 62820

tttactccaa gtactgcgaa ccaaaacgca gaacatcgag tcagatctat actttcgtac 62880

aatcatcgct ttctatctat ctcagattgc ttcatcaatg cgtgcaacta ttgttacacc 62940

gcaccgaggc aaactaccag tgaacatgtt tgtatgtgcg ttaatggagt ctggtgccgg 63000

taaaggttat tcaatgaata tcatggaacg agaaatcgta cagggtttca gtgagacttt 63060

ccgtaaacag acattcccaa acgtatctga gcagtctatt gatgacgaag ctcgcagacg 63120

tgcagttcgg aaccaaactg atgaagctga agaacgtgaa gaactctgga aagagtttaa 63180

ctcactaggt gctatgcctt acagctttga ctctggttca ggcccagcct ataagcaagt 63240

gcgaaccaaa gcacagattg ctggtattgg tgccactaac ttcattggtg atgagatcgg 63300

tactaaccta agtagctgga gcgaaatgct tgcagttaac ttggaagctt acgatgttgg 63360

tttaatcaaa cagaaaatca ctaaaaatgg ttcagactct aaacgttatg aagaacgtag 63420

tgacccagta cctgcaaaca tgctggtatt cggtactcct tctaagctgt ttaacggcgg 63480

cattgaagaa cgtgaattca tttcactatt ggagactggt tatggtcgtc gattcctatt 63540

tggtatcggc aagaagactg tagctcagga tatgacagcc gaggagctgt tcgatatctt 63600

aacgaaccac aacagtgata cgctagttac ccaactggct aaccgtttcc aaacactggc 63660

tgacgagatt aattacaatc gcgagattaa aatctctaaa gatgtaacgt tgattaactt 63720

gcagtaccaa ttggactgtg agaaacgtgc atcttctatg tcggtatttg aaccaattcg 63780

taaagcagaa atgcagcacc gttacttcaa agcattgaag ctggctggtg tatacgcatt 63840

cattgattgc acgcctgaga tcacagaaga acaaatgtat gcggctatta agttagttga 63900

agacagtggt aaagcttgtg atcgtatctt gtcgcaggac aagaactatg tacgactagc 63960

gaagtacatt gcagcagttg gtaaagaagt cacatatgct gacctagttg aagaccttcc 64020

attcttcaat ggttcaaaag cagcgaaaga cgatatgatg tcgttggccc gagcatgggg 64080

gcaccgcaac aatgtgatca ttaccaaaaa gtttgatgat ggatttgaga tcttccgagg 64140

tgaaactcta aaagagactg accttaataa cctgatcttc tccgcatcga accacgttgc 64200

ctataactac atgccttcta cgcacaagaa tctcaaggca gtacgctctt gggagaactt 64260

ccatcgtgtt gctacccaag atggtgtgca ttggtgtaat catactttcg aggatgatca 64320

ccgagtagaa gagaaatcta ttcctgaatt caacatggta gttattgatg ttgatgggga 64380

atgttcgtta gagactgcac agatgctcat gcaagagtac aagtgcttct tctacacaac 64440

caaacgtcac actgacgacg agaaccgttt ccgcattgtg gttccactca aataccactt 64500

gaagatgaca gctaatgagt acaaggagtt catgaacaac ttgtttactt ggctaccatt 64560

caaagttgat gagcaaacga accaacgctg taagaagtgg ttaagtcatg caggaggcga 64620

gtcattgtat aacgatggcg aaatcctaga tccaactcag ttcatcccta agacagccaa 64680

aaatgaaaga cgaatggctg aagctcaaga cattggcgat atggataaag ttgaacagtg 64740

gttcgctcgc tctattgaag agggcaaccg caacaacact attttgaaat acgctttaat 64800

gctcctagac gggggcatgg cccctgacga ggctgaaacc aagactattc aattcaacga 64860

taagttgaag aatggtttgt ctaaagatga gttacgaaca acagtcctta aaaccctatg 64920

ggttaaggca caagaacaag ggaaaatcta atggatttat caaataaatc ttactccgaa 64980

ctggttcacc ttcgcgctca aatcaatcaa gcgattgctg agcaagaaac ctctgtcgtt 65040

gaacgtctga acaaaggcga aatcgtaccg aacttccgtt tcaaagaagg tcgtatgaaa 65100

cgtgcagtta cgaaccaagg tatgttggtt caaacgctac gtgacaaagg tctatctaac 65160

gaagacttgt acgaagctaa gttcgtgggc atcccaacgc tagagaaagt gcttaaagct 65220

aaactatctc aagcagatgc tgacaagatc atgaaacgtc atattgaagt ttcatacggt 65280

aatccaactc ttgagtacac aggtgaataa tggctaagca gaacgacaac ttaatcctcg 65340

tagtagggaa gtcggctgct ggtaagtcga tgtgtcttaa agacatcaaa gatcacgaag 65400

gcgtgatgta cttgaactgt gagtctggta agaaattacc attcaaaaac aacttcaagc 65460

gtctaacaat cactgaccct tacatggtat tccaagcttt ccagaaagct gaagaaatgc 65520

cagagattca tacgattgtt attgactccc ttacgtacct aatggatcta tttgagtccc 65580

tatacgtagt acctaaagct ggcaccaaac aaggtatgca agcttggtct gactacgccc 65640

agttctttaa agcattaatg caacaatatg ttgcagcttc tacaaagaac gttatcatga 65700

ctgctcacac tgctgatgaa gttaatgatg acatggtgaa agaaactttg gttaagatca 65760

aaggttctgt catgaacaac ggcgtagagt cttacttctc acaaatcgtg gcatgtaaga 65820

agatgcagct aaagcatcta gacgggtatg aatctgacct gctgaatatc actgaccgtg 65880

agacacgttt gaagcacaag tatgtgttcc agactgacct aacagcagac acgattaacg 65940

aacgtattcg tggcccattt ggaatgtggg aagaaaacga aatcttcatc gacaacaaca 66000

ttcaattagt actcgatcga attcatgact actacggtga gtagtagctg aaccagatta 66060

ccactatggg gactacgtcc ccttttgcca taagtaacac aggaaagaaa caatatgtta 66120

gacgcactaa aactatcacc agatgttgaa ctagaaaatg ataaagatgt acttggtggc 66180

ggtggcccgc ttaccacaga tatctatgca cttacatgtg agtacatgta tctagagcaa 66240

actaaatctg gtgcagttat ggctgtaggt agcttcaaag aagcagactc aggccgcaac 66300

attcgcttca gcgagtgtgt aatgtctaag aaatcaggta cgttgaaagc aacatacact 66360

gacaagaaga ctaagaaaga gcgtccgtta ccgggctaca ctaaggtact acaccttgct 66420

aaagtgattg gcctagacgt agctgactta tctggtctag cgtgtgaagc gaaagttctt 66480

aaactattcg acttcaaaga gcgtaaagaa gtacctcaag agaagaacgt acttactgac 66540

tttaccggtg cagcacttaa agcaggcgtc tacctagttc gtgaatccaa aatggaagaa 66600

agtgaaccag gtaaatggga tcagccgaca ggtgaagagt tcgctaagaa cgatatcgtt 66660

aagtggttca acgaagatgg cctaaccatc gaagaaatga ccaacggtgt tggtgatgaa 66720

ccatttgctg atcagtggcg tgaagctcac aaaggcaaag aacgcgataa ccgtgtgaaa 66780

gacgcagctc ctaagaaagg tgcaccacaa ctaggtggta ctgctccagc aggcggcggt 66840

cttaacttcg actaatgaat aggggagtag ttgtagctcc cctgcgcatt gctaagaata 66900

aaaagggtga taagcagtgg tcagtgaatt tgaaccaata ccggaataca cacttccaaa 66960

gtcttaatag cttgaagaag cgttataaag atctggtata tcttcaagta gatcaccttt 67020

caccctttac ttgtatccga cttaccattc atatctatgc cgccaataaa cgtctattcg 67080

atataggtaa tgttggttca atacacgaga aattcttttt agatgcactc gttgagttgg 67140

ggaaactacc ggatgacgat tacaatcatt gtcccgaaac ccatacgtac tttaaaggga 67200

tcgacaaaga tcaccctaga gtagaattta ttatcgagga aatcccgatg tcattatttc 67260

aaattgacaa gcctgaccgc atcccagtaa acatccgtac cactgaacat gatttggttc 67320

aattggtcaa agaagctatt gccaaagaag atccttctat cgacgtaact gatgttcgtc 67380

tagttggtct tgaagcgatc gagatcgacg catttgtagg cgaggctcct aagaagaaaa 67440

ctacccgtaa gaccactaag aaagctgaac caaagaaaga agaggttccg gctgaagtgt 67500

taaacgaagt agttgaagca caagaaaaga aagaaactga agctgaaaaa ccagttgaga 67560

ctgcggaaga tacagatcta ccgttcgacc ttgacgatgt tacagaccct gaagcttcaa 67620

gtgctgactt aacttttgcc gaccaagagc tatctctgat tgatgaagtt ctagctgaag 67680

actctaacga gggtgcagta gatgaaccaa tcaacactct aagcttcgac acaaatgaag 67740

aagcagaaag tgaaccagaa acaggtggta cgactgtagc tgatatcttt aagtaatcgc 67800

ctatgttcct gaccattccg tttaacccat tcatgatgct cgctttcatg gtgttctgtt 67860

tagtcgtaat cttcttcggc tatcgaatct ttaaatggtt cagaaacgga atgaaacggg 67920

cattcgatga aatagagaaa gaaaagaagg ggcattaagc cccttttttt attagttagt 67980

gaatggtctt agcaatagag cattgctgat catctctaac gggttgtccg gtaatcttac 68040

acgggctgct agagtatcca acgatagtag tgagtcaatc acacttggaa tagctccacc 68100

agtcattgca ttaagtacgt tgaagataat gtttcgtgca gagttctcac ggaagttacg 68160

gataatctgc ttctgaatac ggaagtagta cttagtgaac caaagtacac ccatatcatt 68220

caagtactgt agcaacggtg ttgtcggcat atcgtagttg ataaacacat ccataaccaa 68280

accagcagca tcacgacgac tcataccatc attctctgtc ttgtgcttgt atagcgcgta 68340

acgagataca aagtctgata gctgagctgc ttcattcatg atcttgaata gcttagtgtc 68400

atgtgacatg taaactacct tggcaccatt tcgaatgaat cccggtaagc gtgtagtcaa 68460

cgcttccact ttctgagtta actgtgactt atgagagaat gtatcatcag ccaattccac 68520

atcttctaca atcgactgca acataccatc ttcaatcaag cttccaacag gactagtcgc 68580

taattcgtga cgcagttcag ccatacgctc ttggttctta tccaagttag ctggttcata 68640

ccccaattct acaattttct ctaactcaaa taactcagca gcgtgacgac ggtaacgacg 68700

tgcttctacg taagcctctt tctgccactt caacgcatca ccaatagaca taccttctaa 68760

gaatagctga gaggtatttg atagagcatt catcagggtt actacacccg acttaactac 68820

cacaaattcc ttagtctctt taacaaattc tttccagata tcttctgctt tacgaatcgt 68880

acctgcggct ttcgggccta atacatggtc agcaaattga accaatgctt cacgtaccca 68940

acttgtctga gctttttcac gatcaagcaa gtcagcagca gatagtttac ggttaccgaa 69000

tacaatcgga gccaaatcac gacgaacata catacggctt acaccccaaa catcgcgagc 69060

atatgcttta gcatctggtg gaagagttgc ccagtattct ttcagaactg gatcttccga 69120

cttagccgat acctcaatgt atgcttcaga attagtagca tcgtattctt cgtatagagc 69180

atcaatcacc tgacggttca cttctggtgt tgtgcgttta tccaaagagt atgcagccat 69240

tgcgcccatt acatcatcaa agtggttatg cttcgctagt agagtatcct tagtctcttc 69300

attcatcacg taacggtaag agttgatcgt accatccgca ttgacgttag gaagtaactg 69360

accaccacgt ttagtagttg ggtcaactcg cttagcctga cgcatctcag caatatcttg 69420

ttgcttagaa cctttgatat ccattgggtc aaactcagtt tgagcagaac ctttcgcctt 69480

acgcgaagta agagagatga tacctgccat gtaagcatta gctccaccgt acttattcac 69540

cattaggtat accggttctg aaccaatgat atgagtatct tgttccagtt cacgtactac 69600

ttggtaacct tgcttttcgt attcagcacg ttcagcagaa ttcacttgaa ccacagagat 69660

attgttatct actaggtcac gggtataacc cttaatgaat tttgaaccat ctccatcaaa 69720

tagagttgct cggtcttcct cttgcatctg cttgtgcata tcaagaacgt aaccaacgct 69780

gtgctcgttt tcacgaatca attgaaccaa tgcagcttta tgctctggtg ccgtgtgttt 69840

gatagcgtac agtgaagcaa gcttatcaat cgtaccaaca tggttttccc aatccgcagg 69900

agtagttgtt acgcctagca tcttagcaat gttctcagca tttagcagag tgtgtgcttc 69960

gtacacgtta cctgttgcca tgtagtaacc aagtgcttgt gatgcacgat agtagaaacc 70020

acggttacgg cttgaaccaa gtagacctag agtacgagag atctctttct ctaactcaac 70080

aggactatca agtactgcct cgatttggtt catatctagg taatcccaaa gtgtagccaa 70140

gtcagtctta ataccggctt tagtaattgc tactttttct tcagagttca gtttacgctt 70200

aaatgaacca ttcagtgctt tagctgtatt cgtttcaatc tgcttacgat cgtggtcaag 70260

tagctttttc atctgacgag ctaaatcatg gaatcgtgca gtgctatctg tacgactcat 70320

tgcctcagtc aacagtgaac caaggatcgt ttgacgacca cctttagaat gcaacttagt 70380

ttgggccaag tattccataa agccaaacac accatcgtat gaccaacgca atacgttact 70440

tgctgtacga accactttga agcggttgtt cttcaagaaa ctcttgttac ccatttcggc 70500

agcagtctca cgagtctcat tagtaaactc gccaagtttg ttagccacaa cgtttaccgc 70560

agcatctgca tattgaacca atttgtgctt gttagctgat tcaactccag ccaattccag 70620

tgccaatttc tgaacacctt cagttactgt catatctgat gttaggccag acaagtgacc 70680

atttacccag ttcagagcag tatcaaagat attacgtaga gtctgaaccc aatcaccttc 70740

aacgaataga tctttagaga atacattctt aggcacttga gtacggctaa gtgcattcat 70800

gaacttctcg ttagtcaaag catacgctgc aaactcttgt aggtagttag aacgaccact 70860

tgcttgagca gtagtacgaa tgttcataac gtagttccaa gtgtctttag cacgcttgta 70920

gtcttctgag tccacaccgt tctgtgggtt atccattaag tcttcaacag ttacaagttt 70980

gttctgctta acataacgga acagtttcat tagctcacgt ttagcacgag agttaccgtc 71040

gatagcagca cgtaaaacac tgtgcgtcat ttcgtgagta aacgtctctt gtgcagacat 71100

ttttgaacca tggaacgcag ctacaccaga tggagttgca accgagtcaa ccatgcgtgt 71160

gtaaatgtaa acgtcatcgc ctctgatagc accataagtg ttacctagtg tatctgctag 71220

gtgtaagtta attggcgtca ttacagggcc aaccagcttg cctaataggt cacgtaggtg 71280

ttcacgatga gtagtactct ctgtcttgtt accaaggtta ttcatttggt caaacgtttg 71340

aaccaagttg tcttggctca ccttctgagt attctctcta gcgaagtcac gggtattgaa 71400

tcgtgcagcc gaagcagaga ataagatatc gtctgctgaa agctctgcat caattacttg 71460

aaccgcagtc attactttct caagagcatt gcctttcttc gcatcagtga taccgaacat 71520

ctctaggatt gcacctttaa cccaagcata agcagacttc actttgtttt cgtaagtgat 71580

ctgtgtttga gccaaagcat tctgcatctg ttttgatgtc agagtccaag cgatcatctc 71640

gtcgatgttg tcgatagagt tagccttgaa tgtatcgcgg tgattcttag ccagttcctt 71700

gcggatgtta gccaaatcat tcttagcttt gttcacttca ggattcttag aatctttcat 71760

tcgctcaact agtgcagcgt gaaccaattc gtgagcaata gtttcatcac ttaggccatt 71820

cactgcgtac tcgtcacctg caataagaat caacttctct ttagcaacat gtaggccacg 71880

gctattaacc attgaaccag agtacttcga gtgtttatct gcatcgaaca attctactgt 71940

gtagtcacgt ggtagtaggt cacctagtga tagtagagtc tgacgaatag cacggtcttt 72000

agaatgtttc ttcaagaacg ctacagtctc aacacttgtt tttgaaccag aagctaagaa 72060

ctctttgata ccttcatcca ctggataaat gttctcttgg ttctcagtcg atactgattt 72120

tggttcaggt ttagcagcat acgtatcgtt caaacgagac actacaaact caagttgagt 72180

tggttcaaat acttcaagag cattcttcac tgtaccggca cggtattcag ttttgaagtt 72240

agccaaagca ttagcaaatg cagtagcacg ctcaccacct tgttcggtat aacgatgcat 72300

tagtcgatca acaacaatgt ccatacgacc tttgttacca gtcataccac ggtggatctt 72360

accgtaagct tcttcataag aaatagtctt tggttcagca cctttattac cagttacgta 72420

gccaccattc tccatgtagt actgagacat gtatgtgata cgtggtaata gcgagttacg 72480

gccttcctcg gtatcagtta ctgtatcgtt gattgaagca aggaagttct cccaaccttt 72540

atcagcagca gttaagttag acgctttaac acgatcaata ccgtatcgag tagcttcttc 72600

gttcactacc attaggctac gggcagtttg ttcagagttt agtacgttag taccattctc 72660

ttcatcgaac ttacgtgcag cttctagagc gcgttgtagc gattcattag cttgagccac 72720

gatagagtaa tcgttcataa cttgattaaa gttacggttg attgcttcca tatctgttag 72780

gccattagcc gtaccagtaa agatcgcatc gtgtacgttt agtacgcctg cattttctag 72840

agtaagtagc attgtcgcag catctgtacc ttgtacagaa agtactgttg gacttacacc 72900

accgtctacc ggagtagtta ctcgaccaaa tgcgttacga gtcttagcac catcaattgc 72960

atacttacct ttacgcttag cctctgtacg gaactgagaa gtctccattg ggatattctc 73020

tgtcttacct acaaagatag cttcgttcaa gttaccagat tgtgctgaga aatatgagtg 73080

gattactggc tgtgcctgtg ccaactcttg ttcaacttca cgcatcatac gcagaggtag 73140

tgtctcacgt ttaccagaga tcagaccttt ctcgatagct tcttgagttt tcttctcgat 73200

aagaatatcg cgttgctgtg catacagatc gaatacgact ttcatcatat cgttcacttt 73260

agtacgacga gccataaact cagaatagtt atcttggata gcgttaaata ccgcttcgcc 73320

atagacttct gaaccaaaat taacaatatc gttttcacga cggtatggta gtttgaaatc 73380

caatgggttc ttaactagct cattagtttg agacttagtt agctgaagca ttgaaccaag 73440

attacgaaga tcttgttctg tacgcttgga gtcgccaacg tagtcataca ctttgtctag 73500

tagcatgtgg cccatagctt tagtgatcgc tttctcacct gaaccataga tagctgtcat 73560

cagtgggttc ttagtcaggt tacgacctgc tttagttacc acttcacgat cagtaccact 73620

tagtgcggca gttagctcac ccattactgt gcttagtaga gctgcatcac gttcacttag 73680

gttgtcgata cgtttaacta catcacgagc aattgcttgg tagttatcat cgttcagtgg 73740

atcagaagac ttccaatcac caaactgttg agtatcgcca tctaggttaa tacctacacg 73800

tttcagacgc tcacctacat tgtcatcaga tgcaccgata gcaaactgta gtgaagcaat 73860

agctgggccg ttagtgatac cgtcaccctc gatagagtaa tcagtttcaa actcagtacg 73920

accttggcgt agagccagca tgtactcaga gtaagcaaca agtgcatcca ttgagtgagc 73980

agcttcacct gctttatcaa ctgtagccag aatcttatcc atatcgaatg gcttgtcttc 74040

tagcatcttg attgtctctt ggataccttc attgaatact ggtgtttgaa ccaattcttc 74100

taattcgtca cgagacattt gatcagtctg tttgtcgatg cttacgccaa agccttgagc 74160

aataccaaca agaaacagat cagtattatc catggaaata gactgacgct gatcaccaac 74220

atatagaaga tgacggtgta gcttatcgcc ttgagggtta accgtattgg agacgatacc 74280

gacacgttgg ttcttccact gttcccagtt gaagtagaac ggttgttctt cgttttccaa 74340

gctttcatgg aactctctcc agtatttaac cgagttcatg atttgaccat tcttaccttc 74400

tacaccagca cgttgatgtt gctgaactgt agcagggtct ttaaggccca tcattcgacc 74460

gatttgttca tctgtcattt gagagaatag tttctgttgg ttcttcttaa caaagtgctc 74520

acgttgtgag tgcttcaata agatatcctg tactttctta gccactgact gacgagtacc 74580

tttctgacga gttactgtct tagttggttt ctcagtagtt gggccacggc ggaatgtttc 74640

agttttgaac agacgaccaa gtacatcatt tgaaccagaa gtatcttcac cgaatgaacg 74700

agcattatca gtgtctacca ttgagtagaa aaagatctca cgattctcat caatatcttt 74760

agctgtgttt gtgtcatcca aaccatcaac aatacgtgcc agttcttttt ggttcacttt 74820

gttaagttgg attaggccat taccacgaag catatctaga ccaaatagac ctaaagatgt 74880

ttctaagtta gtttgggcat tgctacctgc atccgggcca gctttgatac caagagttct 74940

aacaatagct gaaccaagtt catcaatgat gtttgactgg tgagtaccga cgttctgtag 75000

tagatcacgt agctcaggag aaacatactg ttcttcttga cgaccaacaa tgttgttgat 75060

gtcttcttgg ctattaaacg tagtctctgg agctttatga gccatccagt tatacatagc 75120

caaagcaaca gatgctttca cgttctcatc aaatgaacca tcgtcattac gtaggtaaag 75180

catgtaatca gccggtgcat aatcacctgc cgcagcttca tcaaactcag cactaaccgc 75240

tttagcgaat tcagtaaatg aaccaattgc acgagactgt tcttcagtta acgtgtcttg 75300

gtctaaatac tcaactgctt tgttcacatc agctgatagt gtttcaactg cgtttggttc 75360

aacttgaagt gggctattag ttagaccttc ttcagcaaca gtagatacga acttctcacg 75420

aaccacattg gtttcagcaa atgctttatc agcttgctca ggtgaacgtt cttctgtagt 75480

tggttcaaca gtacgagtag ttaagcgttc tgccaactta ggagttggtt cagttacttc 75540

agcttgctcg acagtttcac taataggttc cacatcagca gtagtagtaa catcgctagt 75600

aggagcttca tctacagtaa cctctggttc aatagtagtt tcagtttcaa cagcagccgg 75660

agtctctact tctgtttcca caggagcagc cacgctgtca gtagtatcaa cagcaggagt 75720

ttccacagtt ccattttgta cctcacgtaa accatttagc atagtttgta gtgcagtagc 75780

cgcttctgct tcttgttcaa tctggttcac tagagaaggt gcaatacctt ctgtcttcag 75840

atcaatagaa cgcaatagtt gagcacgttc agcatcatca gtcgtattta gagcctgacg 75900

agccacagca gctttgtttt ggcgttgggc tacaaagtta tcaagatctg taacataacg 75960

ctgtacagca gcctcattgc caccttcgat agcattagaa atgatttcct gatactcgtt 76020

caaaccacga taacccggac taccttcaaa cacgtcttta gatacttcac cgatagattt 76080

attcagacgg ttaaagtcac gtgcagcagt tactacagta cgctgttctt cagttaagtt 76140

agttgaacca agtagagctt cagcgttttc atcgcttaga gattctgggt cgattaggat 76200

agatccaata actcgctcag tagctccagt atcgcctgcc accacttgtt gcgttgcttc 76260

ttcgatgttg tttgtttcaa gttgagcctt ttgtagttga gacatgatat tgctgtaacg 76320

acgaccctca gtacgcaatg cgtcagcacg cttctggaat tgagtcgctt gctcaccctc 76380

ggcagcatta gccgcttctt caagtgtagc aatctgttca gcatagtcag tccatacttt 76440

tacagcagct tcatagttct gtactttctc ttcttgggtt acaccttcaa cattgttgcg 76500

agatgcaata ccacgcagag ttccaccaat tgagaagttt ggatcattga catcagttgt 76560

aacgctctct gttccatttt cttcaatatc ctttttagtt gtacggttct gtttagccgc 76620

tttagcttta tcagttactg aaccagtagc ctcaatgatt gaagcagcag tgttcgcagc 76680

aacgccagaa gcaccacctg atactgcggc agcaaccgca ccagtagcag cagcttcacc 76740

aacaccttca gttaggtctt gggtttcatc taccgtttgt tgtttagcaa tgttagtagc 76800

tacttgtgaa ccaccagatg cacctgtctc ttctaatgct tctttagtag caccttgagc 76860

tgtagcagct aggttactta gtgtcttacc gacacgtgaa gcagggttga acaagttagc 76920

agagaattca ccaccaccag ttagacgaga tgatacgcca gcaaatagtg ccgagtaacc 76980

aaatgccaag ttaccagcag catcttgtag acgagctttc gcttcttcat ggcttaggcc 77040

atctgcaata aggttacgat actcaggaga attttgaacc aattcttcct cagtcatctt 77100

ctcgatttca gtaagtactt gtgcttgtga accaagacct tcttgtactg cagtagttgt 77160

tacacccaaa gcagtagcac gtttcttcag ttctttttga cctgcttctg tagcagccca 77220

cttagcaact gactctgcac cttgctcagc aatttctcgt ggcacagcca aagctttacc 77280

gataccacct gttaggaaga tatcagccac gttctcaaat gctaagtcga tagccaaaga 77340

tggacgactt gcgtatgcag caacagtgtt aaagaattca ccagcaactt ctttagcttt 77400

ctcagtacca gataactctc gatcaccgat ttcagcttta cgttcttcgc ggtatgcttc 77460

cagttcttct tggttagcaa agcgtcgagc ctgaattggt tcagactggg tattattgaa 77520

atcgtcaact agagtctctg catctttaag agcagtaaga atagtattac gttcttcttg 77580

gcgttgttga accagatctt cacgagtctg agtttcacga ccttgctctg ccagtgcagc 77640

ttgtgcacgt tgaaagtaag ggttctctag aacgtcatct acttctgcgt caggtaatgc 77700

agcacctaat gctgattgtc ctaaacgaac tgcaccacga cctgcggcaa tagcagcatc 77760

tgaaccaaat tcagttaatg aacgagtacg atcatctcga cgtaaacggt tctcagcagt 77820

attcgcataa tcacgaatct ccttgagagc agccatagtc ggatctaatt ccaattcaat 77880

ttgattagaa cgttctttaa cttgttgttg ttttgaacca cgtttctgag aaagttcagc 77940

ctgtttctga ctagacttac tattgcccga agccaatgca cggaactgtg ctagttggtc 78000

ttcagtagtg gtatttggtt caaatgtaga agccaagttc tgacgacgtt cttccaaacg 78060

ttgttgaact agttggtctt gcgcagattc ttgttcagga gtgaactgac cttcttcagg 78120

taagtcgatt ggattttggc gttcttcagc cataagataa atctccttga attgatccgt 78180

acattttcga tgtagtgtaa tacgttatca aggagaaata caggaaatgt gaaggggaac 78240

acagttcccc ctctatttag ttaatgtcac ggatggtttt acgcatttct tctactttag 78300

aaggaacatt gtaagtacgc gctcgtaatg cagcaggaac atcccaagct ttaccttgac 78360

ggatttgacg agtcattgct gcctcattct ttttgatttg gctctgtaag tcagccatac 78420

cagcatccat cacgtttaga gcaaggtttg ccttacgtac tgcatcttca tcagtgatta 78480

gattgtagta ctcacgggta gcatctcgga tatttgattc agagagccag cctgtcttat 78540

tacgatcagc tcggcctaag atgtaagcca tctcagcacc agttggttca cgatcaccta 78600

gttcctgttt agcaacacct aagtaatcag caaagacttc tttacccgaa tcaagtgccc 78660

actcaagtcg aggtgcataa cgcttagata ctgtctcaag agaagtctct gtatcgttac 78720

gataattgaa tacttctggt cgagcaccaa gtgtttgagc cgcttcttct tgaatagcct 78780

ccatctcacc ttcgaactgt ttaagagcct gttgaccata acgttcttgg gtttcgtatg 78840

tttgacgttg ttcaggtgac cacgagttgt aatactcttc atggaacttc agagcttcag 78900

tacgaacctt gttagcttct tcagtagaca agtcagtacg tttagctaac tgtgtagtca 78960

ggaagtcacc ataagcatta gcatcaccct caaagcgttt aagcgtctct agagtacgat 79020

agcttgaact tagcaagtct ttctgttgct gaatacctgc tgccgtatta gttgtcttag 79080

gcggcaccgt ataattacca atatgttcta ctcggcctgt attcgggtct tcacgtaaga 79140

tctgaccatt ctcatactta aacccatatt ggttagcctg ttgttgttgc aactggagtt 79200

tacgatcatt caaatcgtta ttgatctctt gctgctccaa cacattctga aacttctgtt 79260

gctccagatt acgatcaatc aaagtatctt ggcgtgaacc aaaaacctga ttaattgctg 79320

tctggtctac ttggttaccg aactgactag agtccacacc acttaaagct tcttgtgaag 79380

tagcgcggtt caactgatca ataatattgg cagtgttacg ctctttctgt gcttcatagt 79440

tagtttggtt cacattggtc tgttcttggg ccaaggaacg taagtcatta attgactgag 79500

agatacgatc agcacctgca tcgaaagcac ccaaggcacc acctgcatca ggtacttgaa 79560

cgttatccca tcggattggt cgagccatgg gttactcctt ataaacgact tttcttctgg 79620

ttgttacgta cttcagcata cgcagcttct tccgcagtac ggcgatcacg ttgatactcg 79680

aactcttcac gttgtagttc ccaagcttca cgctgtagac ctaattggtc tttagcaata 79740

cctagttgct ggaagccgag ccaagagtta gcaagtgtac caacggcacc gatagttgga 79800

gccaaagcac cagtagagaa attaccctgc tcatcagtac caccgaacag agtgttaaat 79860

gtactcatag ggttagtacc ttgagtgttt gaaccaattc cacctgtatc gaaactgtaa 79920

cctgagctag agccgacacc gtagttattg ctgtagttat tgtccattgc taggttaaag 79980

ccttgcatag cgtttgaacc taaaccgtaa tgatttggca tatggagatc tcctatactt 80040

taatgcccat gtgggtagct actccctcaa gggaaatcat gttgtctacg tagttttcaa 80100

tatgtgcttt atcaattgga gccaagttgc ccaagtgaat acgagtagta tagtactgtt 80160

ctgctgtctc atttggaaga gccaagccag accttgaatc cgggatacct aacggatcaa 80220

tcagaaatgc agtacttgtc tcagcataga actggttcat ttcttcttcg tactcattca 80280

tctctcgttg taaagcatcg tagtcatcca tgatatcact catttcagta tcaatgtaag 80340

cgttaacgcc tgtttcgata ccagactgta atgcacttaa cgtttgggcc agacctgtct 80400

ggtcaccacc aactgaacca agagcgatcg aagcgaagat agcaaacaca acaccagcag 80460

ctacaccaag ctcatcgacc aaatagttga taccatattt gataccaacc gtaatgaccc 80520

ctagagcgat ggcacctacc acagccgcag tcaaacctgt accaaggcca agccatgcac 80580

ccatagaggc agaggcagta ccaaaggtat aaatggtcag tacaatacca acaaccgcta 80640

caatggctga gaacaagcct gtttcgtacc atttaagctt ctgtttcaca taacagttga 80700

agttgatacg catagcttcg ttcactaagt cagtacggtc tacaacacgc atattttcaa 80760

ctagacgaat gtccaaaggg ataagcatac cgtctgagaa gtcatcttca ttctgtgccg 80820

cgctaactac gttagctaat gaggtatcta ctgtatgtga tcggtaaaca tagttaatgt 80880

ggttcaaacc acgtacttca acttcatcat atgaattggc agtaatctgc ttacgtacaa 80940

cgaaagaact atcttcataa ccgtagtact gaccagtaac ttgaggacga acaatgatct 81000

cagtgttcac atcatctggt tcagcatctg gttcaattac accagttagc aaagtagatt 81060

taatgtagag ccaacctagc tcaacatcat atgaagtacc tgcctcggaa atacgtaagc 81120

tgttaatcgg tggtggagca gtcagtttga aatcttcatc tctgtcattc cactgatcat 81180

atgcttcttt gtcataggta gagttattct ctttatcttt ccagaagtcg tacaggtagc 81240

tttgtgcagc tttaatctgt gtatttggag agatgcccat taatacgtaa gcatgatcaa 81300

tatcaccaat atccgggtta gcattaaccg cactacctag ttcagagata ctcagcttta 81360

actgtcttag taaacgtcga gaagtgttat agcgttctga accatcatct actgtaagat 81420

cagcattgtt ataccgaaga ggaacaacag gaaagtaatt agagtcaaac tcttcgtaag 81480

gttccagtac ttcataggtg ccctcgtcgg gagaatagaa ccagtaataa ataactgaaa 81540

gatctacttc ctcgtttgca tcgtatttgt agtacccaac gtggtaatac ttagcatctt 81600

ctaagattga accagaatag gtatatgtct cagtgaactc atttaagaat tcatctgtat 81660

aggtaagttc aatttggttc gtactaacga tctttgcgta agagaatact gtttcgcctt 81720

gagcacctga agggggatta cttactacta atgtagtttg gttcaatcct cgtgtaccta 81780

ataagtactc aagagcaaag tagtatccat ctgctgtact gtagattgaa aagtctacaa 81840

gcacatcctc tccgatttct tcagagataa cattctgaac ggtagtaggt gaagcacctt 81900

cattgttcat tgaaccagaa ggcactccat aatagtactc ttcagatccg taccggtaat 81960

atctcttcag gttggttccc aatccactaa tggtattggc aatcaaatca tcggtgatac 82020

tacgttcagc acggatagaa gtaagtactg attgttgaac caaattgggt acttcctcaa 82080

ctagcggagt cgagaaggac tccacataaa ttttagtctt tgaaaataat cccattggtt 82140

caatccttat gcttgtacag ttacgccaac accttcacga actttgttca agacatcttt 82200

gatctgtgta cctgttacgg tagcatccag agtttcgttc tcgtttagag actcgataga 82260

tgtacgggtg ttgaatgagt ccataaatag cttagcagct ttctgctcag catcacggat 82320

gtaaccttca gcttggttct catagagagc catttgctta ccgatgatac cacctgccgc 82380

agcaccaagt actgtgtctt tggtttggta ttcttcagta acacgcttct gtgtaagtac 82440

agcagtttct gcatcaagct tagaaccttg tttaacaatg ttagcttgct ctgcttgtag 82500

gttagcttct tgctgaccag ctagagatgc ttgtgcttct gtctgtagga tttgtgcatc 82560

tttaacttct agatctttat ctaggttaag agcttgctta tccagaatca gttcttgctt 82620

aacagcattc tgcagttctt gatccagtag caattgacgc ttcgtttcag aggttgtttg 82680

ctcattaaca aaggcaatat cggcagttag tttatcaatc tgcttttgaa ccaattctac 82740

attcttctcg gcagcaagaa tctgcttgtc caagagtcgg atgttttggt tcgtaatctg 82800

gtgttgaaga aggtagttaa cagagttagc taaagtacta tctaaagcag caatgtatga 82860

cttggtatac tgatcaccag taatacgtcc ggtcttatgt tcttccgcca gatgagactt 82920

aactgcacgc atcaattcgt caaaggcacc tgaaccagtt ctctcaccag aagtaagtga 82980

gtcaatggtt agtacctctg gatcaaagga tgtaatcgag gtcatctttt ttctcctata 83040

aaaaaggctc cacttattgt gaagcccttt ggttcaaaag tctaccggcc ttagtcgata 83100

gcttgacgtt tagcttggtc agcagctagt gcatctaact cttcttgagt caacggatct 83160

aagatctcaa tgttgtactc tttagggaag aagttcttac gatacttacc acctttacca 83220

tctggaacgt ctttagtctg acggaacact ttgttcttca gtgtatcaat catcatttga 83280

gataggtgat actcgtggtc aaacagaacg tatttagtgt ggaagcctag aactgagtta 83340

ccaatcgaca tccactcacc ttgccattca cgcttgtgag ggttcataca ggtgatacga 83400

cagcgaacaa gtttaagttt gtctttacgt agttgattac gtttatccat cttagtcttg 83460

ttaggggatg ctttaacagt atttgcttca gcagcctttt catcgaattc tttaatcttt 83520

tctttgagtt tctctaaaga gattgaagga tggaatttaa tgcccttgtt ctcggcaatt 83580

tgtttaagac tgtctagttc tgtgaattct tcagacataa tatgtacctt ttagttttgt 83640

atccgttcct gatctaagca ggagggtcaa aggctcccct aaagagagga gccatattta 83700

ttacagttca gctactgtct tagctagagc aatacgctct ggacgtagga tcagtgtacc 83760

gtagtaccac tggattgaac tgaagccagt acgaccgaat gggtcgtggt gttgtgcaac 83820

accttcacca ggcatcttag tgataatctt gaacttgaat gacttaccag aagtttggaa 83880

gccgatagtt gtgaacgatt ctgaaccaat acatagcatt gggaagatat cgtatgaacc 83940

accagtctca cggtaaccac cgttagtagt tactgcttta ccagcaccag cccagtgagt 84000

catctcaggc acaactacga tacggaattg accgacttta ccgatctcac cgtgaatcat 84060

gttcacgcct tttttgtagt cgccagcgta tgcgtactgt tctacaggaa caaatgcttt 84120

gttgccaaat gcatcagtca ttttctcgat ttgcatttgt agttctgaac caacgtaaag 84180

aacacgacct gcaccgatag tagaggtatc agtcatacgt gaaccagtta ggatcttcgt 84240

agacttagga gtacggttgt catccaactg gattgacata cgttgtagat cttcgtaagt 84300

gattactgat ttaacgtcag catcttcacc agtcatttcg atgtcttgag ttgcgtcacc 84360

accgaagtag ataacaccag caccgtttag aaggtcaatt tgtagaacgt cttcagtgat 84420

ctcagatgca ccacgaacca attcacgaga aatgtgttgg tataggtcat ccattgtatc 84480

gaagtttaga gcttcatctg tgaactcatg gaagaaacca aggttctcta gattcgcttc 84540

tagcaccaaa cgtgagtaac caacacggtt cacacgacca ccagtttcag aaagaagtgg 84600

tagcttagcg ttgatagtac cgatatcttt gcttgaacca tataggttac catcagcaat 84660

agttgcaccg gcagcgtcga taccttggtc gttcacgtta cgatcatcta gcaatggaag 84720

catatggtat ttcttaattg tcttaccaaa gtgcttaggc attgctgtta catcagcaag 84780

tggttggaag tactgttctc gaacggcttc aatcagagcc attttgtggt aatagtcctt 84840

gcggatctgt tcaccagcag aagagttagt accgtcaccc cacttaatct ggttagtgtc 84900

atttagagcc atagtaatct cctagtaagt ctctattaaa ttaaagtttg atcttctcaa 84960

actcttcatc agacatattc caccaatcat gtgactcagg ttgtggctta cgtcctttgt 85020

tctgagaaga acttgcctta cggcgtttat cttgcagttg cgtttcatca gctttaggct 85080

tcggtttacg aaccactttc ttaggcttct gttgtggctc ctgtggttgg gagtcattga 85140

acgcaccttg agaaaacaat ttgttaccta ctacctgata tgcctctaca tctgaaaggc 85200

cagctaaacc acctaacatt cgttcacgct gcaactcagt ggaaatcttg tcataaatgc 85260

cattggcttt atgttcattc atgattgaca gcaattgggg tttgcttgca agttcacgct 85320

tactagactc atcccagtta gaaacgacat tcattgtgtc tgaaaatgta ggagtcgagc 85380

gaatgttctc gattacttca tccagttcca cctcattgtc gccaactgca tagttactag 85440

gtgtgtactc tttaccttcc tccagttcca taacgtcaat gccgttagtt tgaaccagtt 85500

gggcaatagc gtctttgtta ccgttcatta aatcaatagc gaatgataac ttggactcgt 85560

caagaagctc attgttggca agcatcttaa cgtacttacg ctgttctttg agatctttag 85620

tcttacgggc ataatccaaa cccatcgaca taagtctacg tgcttcagca acgtctttag 85680

gttgaaactc tgaaccatga tgcttcaaag gagacatcag ttcttcgtac aacttatcaa 85740

attcttcttt tgaaccatct tctgtttcgt cgtctgagtc ttcttcagtc tcatcgtcgt 85800

catcagattc ttgatcatca tcttctgaac cagtttcgtc atcttcgtcg tcactgtcat 85860

cttcatcaga ctctgaatca tcttcttcag aatttggttc aaattcgtct tgatctgtat 85920

cttctgtgat ttctagatcg tcgtcaagac cttctgaacc attttcttct tctggttcaa 85980

aatcatcttc actagaattg tctacagcat caagttcatt attcagttca ccgccttctt 86040

ctcgatcacg gatttcagaa tcaacagaag gctcactagg agcctccgcg aataactgat 86100

ctggatctgc gctcaagaaa tcatcgtctg acatagaata aagattgtta ttcgtagttg 86160

tcataggatt actcctcgtt tactaggtct agcttagttt gctctagttc ttcaagtgca 86220

tgtttggcat gttggccttg catgtgtact tggtggaaga acatttctag ctgagcaaca 86280

ccagtcatac ctagctctgc tgctttacgc tgacgctcgt caggagaagc aaatagtgca 86340

gtaagcgtct taggcttatc ggcaatatac ttcttgagga ttagcttttt aaaagcagaa 86400

ctgcgttcta gtttttctag tgcttcagca agttcaactg cttctttagc gtcttcaatg 86460

gatacttcga tttcttgtag atcagtcatg ttaattactc cgtgtccccg gcatcttgct 86520

cgggattatc ttgaggtgtg ttttggttca aagcatgttc gaatgcttta agtttcatgt 86580

taccttccgc ttgagcttgt tgtttctgta ggtcacgtgc atggttcaca ccattctctt 86640

ctgaaacata atccaagttc ttacgatcgg tatcagcttg tttgttacca gtttctgcca 86700

agatcttctg gatctctgct tgcgtcttct ggatttccat ttgctgagcc tgaatgtcaa 86760

gttgtagctt ctgcatctcc atttgttgtt gtggatctgg ttcagcttca tattgacgaa 86820

ttctctctgc tagttctggc attttacgta aatcagcaat ttctgcaaga attgttttat 86880

acatgcctaa accgaagctt tgaccggttg tttgaagcat aaatgacaat tcctgagctt 86940

tttgctcatc tgcctgtgcc gtactgattg ttaatcttag gtcaaattga cccattaagt 87000

catcacgtct tacagtaaca aacttattat ttgtgatacg gatcacttct tcttcgctta 87060

ggaacgtttg gttcatagca ataatctgac gtgcagcttt cttcaagcca ttagctagac 87120

gacgaagaat acccaattca cgacgagctg ctgcatcaag tgcaccttga gcatgtgtag 87180

ctgtctcacc taaaccagca ccagtaatac cttgtgcgaa tgctttaaca ccagataatg 87240

actcagcttc ttgctgttgc attgtaatca tgttgtatgc agacattggt acttctgggt 87300

attggtgcat atagattgag ttctgtgcat caccaacatc gttatattcg tagtctagac 87360

cagcatcaaa acgacgacgg ttcattgggt ctagggcacc tttacgaata cctgtttgac 87420

cgttagcact acgtgccatt aagtcgatca taccacgagt tactgcgcca attactttct 87480

ggttatcagc gataagttca ccatctggtt caccataaac tgaaccatct acaggaagat 87540

agggaactgt cacaaatgga attttaccat gaggatatgg gttctcttcc agacggatga 87600

tagttgaacc aacccaagta caaacgattg ggcgagtttc acctgaacca tctacgtccc 87660

agtaacccca gtattcataa gctactaatt tctgacgagg tttatccgag aacataaatc 87720

cattttcagc ccagttactt gcatggtcac catcagataa cggtgtgttt gaaccagaaa 87780

cgattgaatc cagattctca taaatacctg ctttacgtag ctcagcaagt gaagtttcaa 87840

acgagtagat aacgaactgt gctttctcaa tatcaccatt acaagatgga tcaataatca 87900

cattctcttg gtcacatact tctagagtcg gttggttcat tgttactaca gtttcttctt 87960

cctcgacttc accagtctta tgaggatata 87990