阅读说明：本技术 维生素b族化合物的稳定性优异的含有维生素b族化合物的酸性组合物 (Vitamin B compound-containing acidic composition having excellent stability ) 是由 喜田香织 朝武宗明 石田亮介 于 2017-12-27 设计创作，主要内容包括：本发明的目的在于,提供一种降低了选自由叶酸、维生素B<Sub>12</Sub>及生物素组成的组中的一种以上的维生素B族化合物的分解、和/或所述维生素B族化合物的稳定性优异的含有所述维生素B族化合物的酸性组合物。一种含有所述维生素B族化合物的酸性组合物,其特征在于,乙醇的含量降低。(The invention aims to provide a food additive with reduced content of vitamin B and folic acid 12 And biotin, and/or an acidic composition containing a vitamin B group compound having excellent stability. An acidic composition comprising said vitamin B group compound, wherein the amount of ethanol is reduced.)

1. A composition characterized in that it comprises, in a first aspect,

is prepared from folic acid and vitamin B₁₂And biotin,

the ethanol content is less than 0.1 wt%.

2. The composition of claim 1, wherein,

the total content of ethanol, glycerol, methanol, acetic acid, formic acid and butylamine is less than 0.1 wt%.

3. The composition of claim 2, wherein,

the total content of the protic organic solvents is less than 0.1% by weight.

4. The composition according to any one of claims 1 to 3,

the composition is a container-packaged beverage or a container-packaged jelly beverage.

5. A food containing a compound selected from folic acid and vitamin B₁₂And biotin, comprising:

the raw materials were blended so that the amount of ethanol was less than 0.1 wt%.

6. The manufacturing method according to claim 5, comprising:

the raw materials are blended so that the total amount of ethanol, glycerin, methanol, acetic acid, formic acid and butylamine is less than 0.1 wt%.

7. The manufacturing method according to claim 6, comprising:

the raw materials are blended so that the total amount of the protic organic solvent is less than 0.1% by weight.

8. The manufacturing method according to any one of claims 5 to 7,

the composition is a container-packaged beverage or a container-packaged jelly beverage.

9. An acidic composition comprising folic acid and vitamin B₁₂And biotin, comprising:

such that the amount of ethanol contained in the composition is less than 0.1 wt%.

10. The method of claim 9, comprising:

the total amount of ethanol, glycerol, methanol, acetic acid, formic acid and butylamine contained in the composition is less than 0.1 wt%.

11. The method of claim 10, comprising:

the amount of protic organic solvents contained in the composition is less than 0.1% by weight in total.

12. The method of any one of claims 9 to 11,

the composition is a container-packaged beverage or a container-packaged jelly beverage.

Technical Field

The invention relates to a method for reducing the content of vitamin B and folic acid₁₂And biotinAn acidic composition containing a vitamin B group compound, which is excellent in the decomposition rate of at least one vitamin B group compound and/or the stability of the vitamin B group compound.

Background

< about folic acid >

Folic acid is one of the essential vitamins of the living body, and folic acid functions as a coenzyme in the living body and is involved in protein biosynthesis and the like. Thus, a deficiency in folate can lead to a variety of diseases, disorders (e.g., megaloblastic anemia, neurological disorders, intestinal dysfunction, etc.). Further, it has been reported that folic acid can be administered to prevent a possible fetal neural tube abnormality due to insufficient nutrition during maternal pregnancy, and that folic acid has a protective effect against cancer, particularly epithelial cancer (non-patent documents 1 and 2). Therefore, folic acid has attracted much attention in recent years.

Recommended amounts of folic acid to be taken are 240. mu.g/day for an adult, 480. mu.g/day for a pregnant woman, and 340. mu.g/day for a lactating woman, according to "diet intake criteria by Japanese (2015 edition)" published by the university of Ministry of labour, Kyoho (non-patent document 3). Folic acid is contained in various foods, but folic acid in foods is almost half lost by cooking and heating, and therefore, it is not easy to ingest a large amount of foods to obtain the recommended ingestion amount.

Therefore, supplements and foods and drinks containing folic acid at a high concentration have been developed and sold, and folic acid in the recommended intake amount can be more efficiently ingested than foods and is therefore popular. In particular, liquid-type supplements and beverages containing folic acid at a high concentration have a high human breath from the viewpoint of ease of ingestion and preference.

On the other hand, folic acid is known to be stable in a dry state, but its stability is lowered in an aqueous solution, particularly in an acidic region suitable for beverages (patent documents 1 and 2). Patent document 1 discloses a method for improving the stability of folic acid in an acidic aqueous solution by forming a complex of folic acid and lactoferrin. Patent document 2 discloses a method for improving the stability of folic acid in a folic acid-containing beverage containing benzoic acids as preservatives by adding an inorganic salt or an organic salt of one or more metals selected from the group consisting of calcium, copper, and zinc to the beverage.

< with respect to vitamin B₁₂＞

Vitamin B₁₂Is one of vitamin B group. Vitamin B₁₂Has effects in improving nervous symptoms such as eye fatigue, myalgia, arthralgia (scapulohumeral periarthritis, lumbago, etc.), neuralgia, and numbness of hand and foot, and is combined with vitamin B₁₂The compositions of (a) are already on the market.

Patent document 3 describes that vitamin B is blended₁And vitamin B₁₂The reasons why the storage stability of both solutions is poor are that the respective stable pH ranges are different and that vitamin B is contained₁The decomposition product of (A) reduces vitamin B₁₂Stability of (2), vitamin B₁₂It is particularly unstable in aqueous sucrose solutions. Further, patent document 3 discloses a method for improving vitamin B₁And vitamin B₁₂An oral liquid for adjusting the pH to 5.8 to 7.5 by stabilizing both of them.

Patent document 4 discloses a method for increasing vitamin B₁And vitamin B₁₂Sugar alcohol is added to stabilize both components, and the pH is adjusted to 3.5-4.5.

Patent document 5 discloses vitamin B₁₂One of cyanocobalamins is unstable in an aqueous solution having a pH of 7.0 to 7.5, but is stabilized by the addition of taurine.

Patent document 6 describes vitamin B₁₂With vitamin B₁、B₂、B₆And ascorbic acid and other vitamins are decomposed and the content is reduced. Further, patent document 6 discloses that vitamin B is used as a means for solving the problem₁₂Vitamin B-containing coating adhered to inert carrier and coated with inert saccharide₁₂The composition of (1).

In patent document 7, vitamin B is used as a stable vitamin₁₂Formulation describes a powdered groupThe compound is characterized in that the compound contains vitamin B₁₂The composition in which the vitamin E-like particles are dispersed in the starch is further dispersed in calcium silicate.

< about biotin >

Biotin is one of the B-group of vitamins, also known as vitamin H or coenzyme E. Biotin catalyzes the carboxylation reaction as a coenzyme of carboxylase in organisms. Carboxylation reactions involve sugar production, branched chain amino acids, fatty acid synthesis, energy metabolism, and the like. Compositions containing biotin are already on the market.

Patent document 8 describes the use of vitamin B for solving the problem of biotin₂The problem of being easily decomposed under coexistence conditions is that the vitamin B and biotin are contained₂The liquid of (2) further contains vitamin C to improve the stability of biotin.

Patent document 9 describes a method of preparing a vitamin composition containing biotin and vitamin B₂The liquid of (2) is further combined with semen Cuscutae extract to improve the stability of biotin.

Patent document 10 describes that biotin is unstable in the presence of a reducing sugar (e.g., granulated sugar) in a liquid for internal use, and therefore it is sometimes preferable to blend sugar alcohol as a sweetener.

Patent document 11 describes that biotin is stabilized by adding stevia extract as a sweetener because biotin is decomposed when biotin is added to an oral liquid preparation at pH2 to 5 containing a sweetener such as granulated sugar.

Patent document 12 describes that biotin is stabilized by adding sucralose to an internal liquid preparation containing biotin.

Patent document 13 describes that biotin is stabilized by adding polyvinylpyrrolidone to an aqueous internal liquid preparation containing biotin.

Disclosure of Invention

The methods for stabilizing folic acid described in patent documents 1 and 2 and non-patent documents 1 to 3 are premised on blending lactoferrin and benzoic acids together with folic acid in a composition, and since there are cases where the use and form of supplements and beverages are limited, there is still a strong demand in this field for a new means capable of improving the stability of folic acid in an acidic aqueous solution.

Vitamin B modification described in patent document 3₁₂In a stabilizing method, in order to make vitamin B₁₂The pH of the liquid for oral administration is required to be adjusted to 5.8 to 7.5 in order to be stable. Vitamin B converting methods described in patent documents 4 to 7₁₂In a stabilizing method, to neutralize vitamin B in the composition₁₂Supplement and drink based on the premise of combining other components for stabilizationThe use and form of the material are limited. In this field, there is still an urgent need for an improvement of vitamin B₁₂Techniques for stability in acidic compositions.

In the methods for stabilizing biotin described in patent documents 8 to 13, the use and form of supplements and beverages may be limited on the premise that other components for stabilization are blended in the composition together with biotin. In this field, a technique for improving the stability of biotin in an acidic composition is still urgently desired.

Accordingly, it is an object of the present invention to provide a composition with reduced levels of a compound selected from the group consisting of folic acid and vitamin B₁₂And biotin, and/or an acidic composition containing the vitamin B group compound, which is excellent in the decomposition rate of at least one vitamin B group compound in the group consisting of the above-mentioned biotin and the stability of the above-mentioned vitamin B group compound.

The present inventors have made intensive studies to solve the above problems and as a result, have found that folic acid and vitamin B are selected as the active ingredients of an acidic composition₁₂And biotin, the rate of decomposition of one or more vitamin B group compounds in the group is increased and/or the stability is decreased due to the presence of ethanol. In addition, it has been found that by reducing the amount of ethanol present in the acidic composition, it is selected from the group consisting of folic acid, vitamin B₁₂And biotin, a reduced rate of decomposition of one or more vitamin B group compounds, and/or an increased stability thereof.

The present invention has been completed based on these findings, and includes the following inventions.

[1]A composition comprising a compound selected from the group consisting of folic acid and vitamin B₁₂And biotin, wherein the content of ethanol is less than 0.1 wt%.

[2] The composition according to [1], wherein the total content of ethanol, glycerin, methanol, acetic acid, formic acid and butylamine is less than 0.1% by weight.

[3] The composition according to [2], wherein the total content of the protic organic solvent is less than 0.1% by weight.

[4] The composition according to any one of [1] to [3], which is a container-packaged beverage or a container-packaged jelly beverage.

[5]A food containing a compound selected from folic acid and vitamin B₁₂And biotin, comprising blending the raw materials so that the amount of ethanol is less than 0.1% by weight.

[6] The method according to [5], which comprises blending the raw materials so that the total amount of ethanol, glycerin, methanol, acetic acid, formic acid and butylamine is less than 0.1% by weight.

[7] According to the method of [6], the raw materials are blended so that the total amount of the protic organic solvent is less than 0.1% by weight.

[8] The method according to any one of [5] to [7], wherein the composition is a container-packed beverage or a container-packed jelly beverage.

[9]An acidic composition comprising folic acid and vitamin B₁₂And biotin, comprising causing the amount of ethanol contained in the composition to be less than 0.1 wt%.

[10] The method according to [9], comprising making the amounts of ethanol, glycerin, methanol, acetic acid, formic acid and butylamine contained in the composition less than 0.1% by weight in total.

[11] The method according to [10], comprising making the amounts of the protic organic solvents contained in the composition less than 0.1% by weight in total.

[12] The method according to any one of [9] to [11], wherein the composition is a container-packed beverage or a container-packed jelly beverage.

The present specification includes the disclosures of japanese patent application No. 2017-247336 and japanese patent application No. 2017-247340, which are the bases of priority of the present application.

According to the present invention, a composition can be provided which is reduced in the amount of a substance selected from the group consisting of folic acid and vitamin B₁₂And biotin, and/or a vitamin B compound having excellent stabilityAn acidic composition of family compounds. According to the present invention, an acidic composition containing the vitamin B group compound, which has excellent storage stability and can be efficiently ingested, can be provided.

Detailed Description

The invention relates to an acidic composition, which is characterized by comprising folic acid and vitamin B₁₂And biotin, and the content of ethanol is reduced.

In the present specification, the term "vitamin B group compound" or "the vitamin B group compound" means "selected from folic acid, vitamin B, as long as it is not particularly limited₁₂And one or more vitamin B compounds selected from the group consisting of biotin.

In one embodiment of the present invention, the active ingredient is selected from folic acid, vitamin B₁₂And biotin is folic acid.

In one embodiment of the present invention, the active ingredient is selected from folic acid, vitamin B₁₂And biotin is selected from the group consisting of vitamin B₁₂。

In one embodiment of the present invention, the active ingredient is selected from folic acid, vitamin B₁₂And biotin is selected from the group consisting of vitamin B₁₂。

One embodiment of the composition of the present invention is an acidic composition characterized by containing folic acid and having a reduced content of ethanol.

One embodiment of the composition of the present invention is an acidic composition characterized by containing vitamin B₁₂And the content of ethanol is reduced.

One embodiment of the composition of the present invention is an acidic composition characterized by containing biotin and having a reduced ethanol content.

In the present invention, "folic acid" is one of the water-soluble vitamins of the vitamin B complex, and means a compound represented by the following structural formula called pteroylmonoglutamic acid,

[ chemical formula 1]

Or a derivative thereof or a salt thereof which is acceptable in pharmaceuticals, foods and beverages. Examples of the derivative include, but are not limited to, polyglutamic acid type derivatives to which a plurality of glutamic acids are bonded.

Folic acid can be extracted or purified from foods, or chemically synthesized, and examples of foods containing folic acid include, but are not limited to, asparagus, broccoli, spinach, green soy bean, broad bean, corn, mini cabbage, kale, garlic, camomile and other vegetables, litchi, strawberry, mango, avocado, durian and other fruits, chicken liver, beef liver, pork liver and other meats, and folic acid can be synthesized by a known method (eighth version of the food additive, written book, pages D-1655-D-1660, kayak store, 2007, 12.10 days), for example, by maintaining an aqueous molar solution of 2,4, 5-triamino-6-hydroxypyrimidine, p-formylglutamic acid at pH4, adding an ethanol solution of α -dibromopropionaldehyde, condensing the aqueous solution, dissolving the aqueous solution in an aqueous solution of pH9 or more, and then adjusting the pH to 7 to remove insoluble folic acid (this method).

In one embodiment, the composition of the present invention comprises folic acid. In the composition of the embodiment containing folic acid, folic acid can be appropriately contained in an amount selected from the range of 0.1 to 10ppm, preferably 1 to 10ppm, and more preferably 1 to 5 ppm. For example, the composition of the present embodiment may contain folic acid in an amount of 100 μ g or more, 150 μ g or more, 200 μ g or more, 240 μ g or more, 250 μ g or more, 300 μ g or more, 350 μ g or more, 400 μ g or more, 450 μ g or more, 500 μ g or more, and 550 μ g or more, as appropriate, when taken orally at one time. For example, the range of the intake amount (days) of folic acid recommended in "japanese diet intake standard (2015 edition)" published by the ministry of health and labor is preferably included. The upper limit of the amount of folic acid contained in the amount taken per one time is not particularly limited, and can be appropriately determined from the range of, for example, 1000. mu.g or less, 900. mu.g or less, 800. mu.g or less, 700. mu.g or less, and 600. mu.g or less.

In the present specification, the term "one-time oral intake amount" refers to the amount of the above-mentioned composition to be orally taken at one time, or the total amount to be orally taken a plurality of times at short time intervals (for example, 10 minutes or less, preferably 5 minutes or less) consecutively, and in the case where the composition is in a liquid or semisolid (gel, sol or the like) form, for example, 50m L to 500m L (typically, 50m L0, 100m L1, 150m L2, 180m L, 200m L, 250m L, 300m L, 350m L, 400m L, 450m L, or 500m L) is the amount thereof.

In the present invention, "vitamin B₁₂"is one of the water-soluble vitamins of the vitamin B complex, and is a generic term for vitamins containing cobalt. Vitamin B₁₂Specifically, the compound contains hydroxycobalamin, cobamamide, methylcobalamin, cyanocobalamin, thiocobalamin, or a derivative or salt thereof which is acceptable in pharmaceutical products, foods and beverages.

Vitamin B₁₂Can be extracted or purified from food, or produced by microorganism, or chemically synthesized. As containing vitamin B₁₂The food (b) is not particularly limited, and examples thereof include fish and shellfish such as oyster, clam, salmon roe, pacific saury, and herring, and meat such as chicken liver, beef liver, and pork liver.

Vitamin B₁₂Cyanocobalamin in (b) can be isolated from a culture solution of actinomycetes (Streptomyces) or bacteria (Agrobacterium, Bacillus, Flavobacterium, Propionibacterium, Rhizopus).

In one embodiment, the composition of the invention comprises vitamin B₁₂. Contains vitamin B₁₂The composition according to the embodiment of (1) may contain vitamin B in an amount selected from the range of 0.001ppm to 5ppm, preferably 0.005ppm to 0.5ppm, more preferably 0.01ppm to 0.05ppm₁₂. For example, the composition of the present embodiment can be orally ingested in an amount of 0.9. mu.g or more, 1.0. mu.g or more, 1.2. mu.g or more, 1.5. mu.g or more, 1.8. mu.g or more, 2.0. mu.g or more, 2.1. mu.g or more, 2.2. mu.g or more, 2.3. mu.g or more, 2.4. mu.g or more per one timeAbove, 2.5. mu.g or more, 3.0. mu.g or more, and 3.2. mu.g or more, vitamin B is appropriately contained₁₂. For example, vitamin B recommended in "diet intake Standard of Japanese (2015 edition)" published by the Ministry of health and labor of the university is preferably contained₁₂The amount (day) of intake. Vitamin B contained in a single oral intake₁₂The upper limit of the amount of (B) is not particularly limited, and can be appropriately determined from the range of, for example, 10. mu.g or less, 9. mu.g or less, 8. mu.g or less, 7. mu.g or less, 5. mu.g or less, and 4. mu.g or less.

In the present invention, "biotin" is one of the essential water-soluble vitamins, and means a compound represented by the following structural formula:

[ chemical formula 2]

Or a derivative or a salt thereof which is acceptable in a pharmaceutical product, a food or a drink.

Biotin can be extracted or purified from food, or chemically synthesized. The biotin-containing food is not particularly limited, and examples thereof include beans and grains such as beef liver, egg yolk and soybean.

In one embodiment, the composition of the invention comprises biotin. In the composition of the embodiment containing biotin, biotin can be appropriately contained in an amount selected from the range of 0.001ppm to 10ppm, preferably 0.01ppm to 1ppm, and more preferably 0.2ppm to 0.3 ppm. For example, the composition of the present invention may contain biotin in an amount of 10 μ g or more, 15 μ g or more, 20 μ g or more, 25 μ g or more, 30 μ g or more, 35 μ g or more, 40 μ g or more, 45 μ g or more, 50 μ g or more, 60 μ g or more, 70 μ g or more, or 100 μ g or more, which is taken orally at one time. For example, the range of the intake amount (days) of biotin recommended in "diet intake standard of japanese (2015 edition)" published by the ministry of health and labor is preferably included. The upper limit of the amount of biotin contained in the amount taken orally at one time is not particularly limited, and can be appropriately determined from the range of, for example, 1000. mu.g or less, 500. mu.g or less, 400. mu.g or less, 300. mu.g or less, 200. mu.g or less, and 150. mu.g or less.

In the present invention, the "protic organic solvent" refers to an organic solvent that dissociates itself to generate protons, and refers to a substance having a hydrogen atom bonded to a nitrogen atom or an oxygen atom, which is an atom having a large electronegativity. Examples of such a protic organic solvent include, but are not limited to, lower alcohols (ethanol and methanol), glycerin, acetic acid, formic acid, and butylamine.

As detailed in the examples below, ethanol has the effect of increasing the rate of decomposition of the vitamin B group compound in acidic compositions. Therefore, by reducing the amount of ethanol contained in the acidic composition, the decomposition rate of the vitamin B group compound in the acidic composition can be reduced, and the storage stability of the vitamin B group compound in the acidic composition can be improved. Since glycerin, methanol, acetic acid, formic acid, and butylamine, which are protic organic solvents, as well as ethanol, have the effect of increasing the decomposition rate of the vitamin B group compound, it is preferable that the total amount of ethanol, glycerin, methanol, acetic acid, formic acid, and butylamine contained in the acidic composition be reduced to reduce the decomposition rate of the vitamin B group compound in the acidic composition and improve the storage stability of the vitamin B group compound in the acidic composition. In particular, it is preferable that the total amount of the protic organic solvent (including ethanol, glycerol, methanol, acetic acid, formic acid, butylamine, and the like) contained in the acidic composition is reduced, whereby the decomposition rate of the vitamin B group compound in the acidic composition can be reduced, and the storage stability of the vitamin B group compound in the acidic composition can be improved.

In order to reduce the decomposition rate of the vitamin B group compound and/or improve the storage stability, the amount of ethanol contained in the composition of the present invention is preferably reduced as much as possible, and the storage stability of the vitamin B group compound in the composition is improved as the content of ethanol contained in the composition is reduced. For example, by reducing the amount of ethanol contained as compared with a conventional acidic composition containing the vitamin B group compound, a composition having a reduced decomposition rate of the vitamin B group compound and/or high storage stability as compared with a conventional acidic composition can be obtained. Therefore, the amount of ethanol contained in the composition of the present invention is not particularly limited as long as it is smaller than the amount of ethanol in the conventional acidic composition containing the vitamin B group compound, and specifically, it can be set in a range of less than 0.1 wt%, for example, less than 0.09 wt%, less than 0.08 wt%, less than 0.07 wt%, or less than 0.06 wt%. Particularly preferably, the content can be set to a range of 0.05 wt% or less, 0.04 wt% or less, 0.03 wt% or less, 0.02 wt% or less, or 0.01 wt% or less.

In order to further reduce the decomposition rate of the vitamin B group compound and/or further improve the storage stability, the total amount of ethanol, glycerol, methanol, acetic acid, formic acid and butylamine in the composition of the invention is preferably reduced as much as possible. The total amount of ethanol, glycerin, methanol, acetic acid, formic acid, and butylamine in the composition of the present invention is not particularly limited as long as it is less than the total amount in the conventional acidic composition containing the vitamin B group compound, and specifically, it can be set in a range of less than 0.1 wt%, for example, less than 0.09 wt%, less than 0.08 wt%, less than 0.07 wt%, or less than 0.06 wt%. Particularly preferably, the content can be set to a range of 0.05 wt% or less, 0.04 wt% or less, 0.03 wt% or less, 0.02 wt% or less, or 0.01 wt% or less.

In order to further reduce the decomposition rate of the vitamin B group compound and/or further improve the storage stability, the total amount of the protic organic solvent comprising ethanol, glycerol, methanol, acetic acid, formic acid, butylamine, and the like in the composition of the present invention is preferably reduced as much as possible. The total amount of the protic organic solvent in the composition of the present invention is not particularly limited as long as it is less than the total amount in the conventional acidic composition containing the vitamin B group compound, and specifically, it can be set in a range of less than 0.1 wt%, for example, less than 0.09 wt%, less than 0.08 wt%, less than 0.07 wt%, or less than 0.06 wt%. Particularly preferably, the content can be set to a range of 0.05 wt% or less, 0.04 wt% or less, 0.03 wt% or less, 0.02 wt% or less, or 0.01 wt% or less.

The terms "ethanol", "ethanol, glycerin, methanol, acetic acid, formic acid, and butylamine" and "protic organic solvent" in the present invention include not only those incorporated during the production of the acidic composition of the present invention, but also those incorporated/mixed during the production of the raw materials used for the production of the composition. For example, almost all types of perfumery formulations currently contain ethanol (Pinus, et al, journal of food hygiene, Vol.38, No.6, pp.452-459, December, 1997). However, although a diluent to be blended in a final product is shown in a commercially available flavor preparation, the diluent may not be shown in the case of a solvent used for extraction of a flavor, and a solvent contained in a flavor before blending may be shown in the case of blending a plurality of flavors in the preparation of a flavor preparation. Therefore, even in the case where the inclusion of ethanol in the flavor preparation is not shown, ethanol may be actually included, and in the case where such a flavor preparation is incorporated into the composition of the present invention, the content of ethanol in the composition can be increased, thereby increasing the decomposition rate of the vitamin B group compound.

The amounts of "ethanol", "ethanol, glycerin, methanol, acetic acid, formic acid, and butylamine" and "protic organic solvent" in the composition of the present invention can be measured by conventionally known general methods. For example, the content of each solvent component in the composition can be determined by mixing the composition with anhydrous sodium sulfate and acetonitrile, filtering the mixture, dissolving the obtained filtrate in acetonitrile, and subjecting the resulting solution to gas chromatography as an analysis sample (Yixianshi, journal of food and health, Vol.26, No.1, pp.39-45,1985).

In the present invention, "acidic composition" means a composition having a pH of 4.5 or less, and can be set to, for example, a pH of less than 4.5, a pH of less than 4.4, a pH of less than 4.3, a pH of less than 4.2, a pH of less than 4.1, a pH of 4 of less than.0, a pH of less than 3.9, a pH of less than 3.8, a pH of less than 3.7, a pH of less than 3.6, a pH of less than 3.5, a pH of less than 3.4, or a pH of less than 3.3. The lower limit of the pH value is not particularly limited, and can be appropriately determined depending on the form of the acidic composition, and can be set to, for example, pH2.5 or more, pH2.6 or more, pH2.7 or more, pH2.8 or more, pH2.9 or more, pH3.0 or more, pH3.1 or more, or pH3.2 or more.

In the case where the acidic composition of the present invention is in the form of a refreshing beverage, the pH is preferably less than pH 4.0. The sterilization and degerming methods for refreshing beverages are widely different from those of ph4.0 in accordance with the standards of food category specifications of the food sanitation act, and sterilization of beverages with ph less than ph4.0 requires heating the center portion at 65 ℃ for 10 minutes, whereas sterilization of beverages with ph4.0 or more requires heating the center portion at 85 ℃ for 30 minutes. Therefore, it is preferable that the upper limit of the pH value is less than pH4.0, because the load of the sterilization step can be reduced, and the decomposition of the vitamin B group compound in the sterilization step can be reduced. In the present specification, the pH value means a value measured at a product temperature of 20 ℃.

The pH of the acidic composition of the present invention can be adjusted by appropriately adjusting the amount of the acidulant added. Examples of the acidulant include those commonly used in the production of medicines and foods and beverages, and examples thereof include citric acid, malic acid, gluconic acid, tartaric acid, lactic acid, succinic acid, and salts thereof, and one or a mixture of two or more of these acids can be added.

In the acidic composition of the present invention, other raw materials that are acceptable as medicines or foods, excipients, disintegrators, lubricants, binders, antioxidants, coloring agents, anti-aggregation agents, absorption promoters, solvents, dissolution aids, isotonic agents, stabilizers, taste-modifying agents, pH adjusters, flavor preparations, sweeteners, thickeners, preservatives, vitamins, agar, and the like can be appropriately selected and blended according to the form required in the composition, in addition to the vitamin B group compound. However, it is preferable to use a component containing no ethanol or a low content of ethanol, more preferably a component containing no ethanol, glycerol, methanol, acetic acid, formic acid, and butylamine or a component containing a low total content thereof, and particularly preferably a component containing no protic organic solvent (including ethanol, glycerol, methanol, acetic acid, formic acid, and butylamine) or a component containing a low total content of protic organic solvents. For example, as noted above, almost all types of fragrance formulations currently contain ethanol as a solvent. In the present invention, a fragrance preparation using one or more selected from medium-chain fatty acids and/or emulsifiers instead of ethanol as a solvent can be contained instead of such a fragrance preparation. The medium-chain fatty acid may be, but not limited to, C8, C10, or C12 triglyceride, and the emulsifier may be, for example, glycerin fatty acid ester, sorbitol fatty acid ester, propylene glycol fatty acid ester, or sucrose fatty acid ester.

The acidic composition of the present invention can be provided in the form of a pharmaceutical (including a quasi drug) or a food or beverage. These forms may be liquid compositions or semisolid compositions (gels, etc.), and the vitamin B group compound and other raw materials may be appropriately blended according to the form of the preparation, and the preparation may be carried out according to a conventional method.

The container for containing the acidic composition (liquid composition) of the present invention can be suitably used as a container for beverages, and is not limited, and a container for beverage, a container made of polyethylene terephthalate (PET), a so-called PET bottle, a metal can container, and the like can be mentioned, and the form of the container is not particularly limited, and the capacity of the container is not particularly limited, and can be set to, for example, 50 to 500m L (typically 50m L, 100m L0, 150m L1, 180m L2, 200m L, 250m L, 300m L, 350m L, 400m L, 450m L, or 500m L), and preferably 100 to 200m L.

The container for containing the acidic composition (semisolid (gel, sol, etc.) composition) of the present invention can be suitably used as a container for jelly beverages, and is not limited, and a container made of a resin film and/or a metal film, a bag container, and the like can be used, and the capacity of the container is not particularly limited, and can be set to, for example, 50m L to 200m L (typically, 50m L, 100m L, 150m L, 180m L, and 200m L).

The acidic composition of the present invention can be produced as follows: the vitamin B group compound extracted or purified from a food or the chemically synthesized vitamin B group compound, an acidulant, the other raw materials as needed, and water as the remainder are mixed, and the content of ethanol is made smaller than that of ethanol in a conventional acidic composition containing the vitamin B group compound, specifically, made to be less than 0.1 wt%, for example, less than 0.09 wt%, less than 0.08 wt%, less than 0.07 wt%, or less than 0.06 wt%, preferably 0.05 wt% or less, 0.04 wt% or less, 0.03 wt% or less, 0.02 wt% or less, or 0.01 wt% or less. The acidic composition of a more preferred embodiment of the present invention can be produced as follows: the vitamin B group compound extracted or purified from a food or the chemically synthesized vitamin B group compound, an acidulant, the other raw materials as needed, and water as the rest are mixed, and the total content of ethanol, glycerin, methanol, acetic acid, formic acid, and butylamine is made smaller than the content of ethanol in a conventional acidic composition containing the vitamin B group compound, specifically, made in a range of less than 0.1 wt%, for example, less than 0.09 wt%, less than 0.08 wt%, less than 0.07 wt%, or less than 0.06 wt%, preferably 0.05 wt% or less, 0.04 wt% or less, 0.03 wt% or less, 0.02 wt% or less, or 0.01 wt% or less. The acidic composition of a particularly preferred embodiment of the present invention can be produced as follows: the vitamin B group compound extracted or purified from a food or the chemically synthesized vitamin B group compound, an acidulant, the other raw materials as needed, and the balance water are mixed so that the total content of protic organic solvents is less than the total content of protic organic solvents in a conventional acidic composition containing the vitamin B group compound, specifically, in a range of less than 0.1 wt%, for example, less than 0.09 wt%, less than 0.08 wt%, less than 0.07 wt%, or less than 0.06 wt%, preferably in a range of 0.05 wt% or less, 0.04 wt% or less, 0.03 wt% or less, 0.02 wt% or less, or 0.01 wt% or less. The means for storing and sterilizing the acidic composition of the present invention in a container can be selected arbitrarily.

The present invention will be described in further detail below with reference to examples and test examples, but the present invention is not limited to these examples.