阅读说明：本技术 抗衰老健康食品和化妆品以及源自葡萄籽的抗衰老成分的制造方法 (Anti-aging health food and cosmetic and method for producing anti-aging ingredient derived from grape seed ) 是由 永尾司 于 2017-12-15 设计创作，主要内容包括：问题：提供比白藜芦醇等已知的预计具有抗衰老作用的物质,具有更优异的抗衰老作用的抗衰老健康食品和化妆品以及具有优异的抗衰老作用的源自葡萄籽的抗衰老成分的制造方法。解决方案：本发明的抗衰老健康食品和抗衰老化妆品,含有经过粗提纯的源自葡萄籽的抗衰老成分,该成分包含60重量％以上的源自葡萄籽的多酚。(The problems are as follows: provided are anti-aging health foods and cosmetics having more excellent anti-aging effects than known substances expected to have anti-aging effects such as resveratrol, and a method for producing an anti-aging component derived from grape seeds having excellent anti-aging effects. The solution is as follows: the anti-aging health food and the anti-aging cosmetic of the present invention contain a crude purified anti-aging ingredient derived from grape seeds, which contains more than 60% by weight of polyphenols derived from grape seeds.)

1. An anti-aging health food and cosmetic characterized by: contains a crude purified anti-aging component derived from grape seeds, the component comprising more than 60% by weight of polyphenols derived from grape seeds.

2. A method for producing an anti-aging ingredient derived from grape seeds, comprising the steps of:

step 1, selecting more than one grape seeds from European grapes (Vitis vinifera L.), American grapes (Vitis labrusca L), purple grapes (Vitis coignetitae L), amur grapes (Vitis amurensis L) and white grapes (Vitisshiragai L) for pretreatment, and drying the grape seeds in a germination accelerating state at 35-60 ℃;

step 2, powdering the grape seeds dried in the step 1;

step 3, immersing the grape seed powder obtained in the step 2 in one of water, ethanol, or a mixed solvent of water and ethanol to obtain an extract fraction containing polyphenols derived from grape seeds;

and 4, drying and powdering the part of the extract containing the polyphenol derived from the grape seeds obtained in the step 3.

3. The anti-aging health food and cosmetic according to claim 1 or the method for producing the anti-aging ingredient derived from grape seeds according to claim 2, characterized in that:

the grape seed is selected from the group consisting of Alzheimer's (Agiorigitio), Vioni (Viogenier), Cabernet Sauvignon, Pinelizhu (Cabernet Franc), Jiamei (Gamayy), Jialina (Carignan), Jiamene (Carmenere), Heixinuo (Xinomaro), Geena (Grenache), Qiongyao slurry (Gewurztraminer), Kerner (Kerner), Columbard (Colombard), Jiazhou, Sudana (Sultana), Mulvesse (Gigigigere), Sandonnay (Chardonnay), white south (Chennin Blanc), Sira (Syrah), Pinnfandel (Zinfandel), Sarron (Selon), Abrus (Juniguan), Pirani (Black), Pirani (Pirani), Pirani (Pinilla), Pinilla (Pinilla), Pinolo (Pinolo), Pinolo (Pinilla), Pirani (Pinolo), Pinolo (Pinolo), Pirani (Piano (Pinilla), Pirolo), Pirani (Pirani), Pirani (Pirani), Pinolo), Pirani (Piano (Pirani), Pimpi), Pinolo (Pirani), Pirani (Pirani), Pirani (Pirani, Seeds of more than one species of vitis vinifera from the group of bailey a musk (MuscatBailey a), marbeck (Malbec), Muller-churau (Muller-Thurgau), mujjust (Mourvedre), mennier (meinier), burgundy Melon (Melon de Bourgogne), Merlot (Merlot), moschatato (moscatio), sauvignon rubra, lein (riegling) and Ruby Cabernet (Ruby Cabernet).

Technical Field

The present invention relates to anti-aging health foods and cosmetics and a method for producing anti-aging ingredients derived from grape seeds, and more particularly, to anti-aging health foods and cosmetics having an anti-aging effect superior to that of resveratrol and a method for producing anti-aging ingredients derived from grape seeds having an excellent anti-aging effect.

Background

According to studies on prevention of aging and control of lifespan to date, compounds expected to have an anti-aging effect, such as resveratrol (trans-3, 4', 5-trihydroxystilbene), have been found.

For example, non-patent document 1 describes a study result that resveratrol, in Saccharomyces cerevisiae (Saccharomyces cerevisiae), stimulates Sir2 of an enzyme of Caenorhabditis elegans (Caenorhabditis elegans) that regulates longevity to improve stability of Saccharomyces cerevisiae and extend the longevity of Saccharomyces cerevisiae by 70%.

On the other hand, however, as described in non-patent document 2, there is also reported a study result that resveratrol does not prolong the lifespan of male and female mice and thus resveratrol does not have an anti-aging effect.

Thus, although resveratrol is expected to have an anti-aging effect, it has not been actually determined whether resveratrol has an anti-aging effect.

[ Prior art documents ]

[ non-patent document ]

Non-patent document 1 Konrad T.Howitz et al, Nature 425, 191-196(2003) non-patent document 1 Richard A.Miller et al, Journal of Gerontology BIO L OGICA L SCIENCES 191-201(2010)

Disclosure of Invention

Problems to be solved by the invention

As described above, it has not been determined whether resveratrol, which is expected to have an anti-aging effect, actually has an anti-aging effect. Thus, there is a need for a more defined substance with anti-aging effects superior to resveratrol.

Through intensive research, the inventors found that a polyphenol extract derived from grape seeds has an anti-aging effect superior to that of resveratrol.

The present invention addresses the problem of providing an anti-aging component derived from grape seeds that has an anti-aging effect superior to chenopodium album alcohol, a method for producing the same, and an anti-aging health food and an anti-aging cosmetic containing the anti-aging component.

Means for solving the problems

The present invention according to claim 1 relates to anti-aging health foods and cosmetics characterized by containing a roughly purified anti-aging component derived from grape seeds, which contains more than 60% by weight of polyphenols derived from grape seeds.

Invention claim 2 relates to a method for manufacturing an anti-ageing component derived from grape seeds, comprising the steps of:

step 1, selecting more than one grape seeds from European grape (vitas vinifera L.), American grape (vitas labrusca L.), purple kudzu grape (vitas coignetiae L.), Vitis amurensis L and white congratulation grape (vitas shiragai L), pretreating, and drying the grape seeds in a germination accelerating state at 35-60 ℃;

step 2, powdering the grape seeds dried in the step 1;

step 3, immersing the grape seed powder obtained in the step 2 in one of water, ethanol, or a mixed solvent of water and ethanol to obtain an extract fraction containing polyphenols derived from grape seeds;

and 4, drying and powdering the part of the extract containing the polyphenol derived from the grape seeds obtained in the step 3.

The present invention according to claim 3 relates to the antiaging health foods and cosmetics as set forth in claim 1 or the method for producing a grape seed-derived antiaging ingredient as set forth in claim 2, characterized in that the grape seed is selected from the group consisting of aggirik (Agiorgitiko), veroni (Viornier), Sauvignon (Cabernet Sauvignon), pinkis (CabernetFranc), Jiamei (Gamayy), Jialian (Carignan), Jiamena (Carmenere), Black Xiono (Xinomairo), Geena (Grenache), Johno pulp (Gewurztraminer), Kerner (Kerner), Colomo (Colomo), Sulta (Sultana), Sancojevignon (Sangiove), Durioli (Chardonnay), white south (Cheranc), Blira (Blania), Sanbaronary (Blani), Sanbaronary (Syranolo), Sanbaro (Piracy), Sanvial), Sandalo (Piracy), Sandale (Piracon), Piracon (Nao), Piracon (Nao), Piracon (Piracon), Piracon, Babela (Barbera), pinotagi (Pinotage), melanopino (Pinot Noir), chenopodium (Pinot Gris), pinoponto (Pinot Blanc), Petit Verdot (Petit Verdot), Queen Black (Black Queen), belley a musk (Muscat Bailey a), marbeck (Malbec), Muller-churu, mujvate (Mourvedre), mennier (meinier), burgh Melon (Melon de Bourgogne), meroro (Merlot), moschatato (moscator), sauvigna rubra, leist (riegling), and ruby within the ruby caber (ruby caber).

Effects of the invention

According to the invention of claim 1, since the anti-aging health food and cosmetics contain the crude-purified anti-aging ingredient derived from grape seeds, which includes more than 60% by weight of polyphenols derived from grape seeds, excellent anti-aging effect is obtained, and the anti-aging ingredient can be used for the anti-aging health food and cosmetics.

According to the invention of claim 2, the method for producing the antiaging ingredient derived from grape seeds comprises the steps of 1, pretreating one or more grape seeds selected from Vitis vinifera (Vitis vinifera L.), Vitis americana (Vitis labrusca L.), Vitis corni (Vitis coigneniae L), Vitis amurensis (Vitis amurensis L) and Vitis vinifera (Vitis shiragai L), drying the grape seeds in a sprouting state at 35 to 60 ℃, 2, powdering the grape seeds dried in the step 1, 3, immersing the grape seed powder obtained in the step 2 in one of water, ethanol, or a mixed solvent of water and ethanol to obtain an extract fraction containing polyphenols derived from the grape seeds, and 4, drying and powdering the extract fraction containing the polyphenols derived from the grape seeds obtained in the step 3.

According to the invention of claim 3, the anti-aging health food and cosmetic as set forth in claim 1 or the method for producing an anti-aging ingredient derived from grape seed as set forth in claim 2 is selected from the group consisting of aggirtike (Agiorgitiko), veouri (Viogenier), Cabernet (Cabernet Sauvignon), pindol (Cabernet Franc), camet (Gamay), Canianna (Carignnan), Canmenanere (Carmenere), Xiunovoro (Xinomaro), henna (Grenache), Yao slurry (Gewurzzramine), Kerner (Kerner), Colombard (Colombard), Jiazhou, Sulta (Sulta), Sangiove (Sangiove), Chardonnay (Chardonnay), white south China (Cheranc), Wenyana (Symilah), Blira (Blulan), Nevira (Black), Sannilla (Avulan), Pingeon (Avulan), Pinna (Piraco), Pinna (Avulan), Pinna (Piraco), Pingyona (Piraco), Pink ' (Piraco), Pinna (Piraco), Pingyun (Piraco), Pink ' (Piraco), Pingyun) and Piraco (Piraco), Pingylco (Piraco), Pincear ' (Piraco), Pincear, Babela (Barbera), pinotagi (Pinotage), melanopino (Pinot Noir), chenopodium (Pinot Gris), pinoponto (Pinot Blanc), Petit Verdot (Petit Verdot), Queen Black (Black Queen), belley a musk (Muscat Bailey a), marbeck (Malbec), Muller-churu (Muller-Thurgau), muurvedre (Mourvedre), mennier (meinier), burgh Melon (Melon de Bourgogne), meroro (Merlot), moschatato (moscator), sauvigna rubra, lei (rieng), and seeds of more than one species of wine grape within ruby cabber (ruby caber), so that a healthy food and cosmetic having more excellent anti-aging effects can be provided, or ingredients derived from anti-aging can be made more excellent.

Drawings

FIG. 1 is a graph showing the amounts of senescent cells calculated in examples 1 to 3, comparative example 1 and control group. (a) Is the amount of senescent cells in cells cultured for 7 days, (b) is the amount of senescent cells in cells cultured for 14 days, and (c) is the amount of senescent cells in cells cultured for 21 days. The amount of senescent cells in examples 1 to 3 and comparative example 1 was expressed as a ratio to the amount of senescent cells in the control group, with the amount of senescent cells calculated in the control group being 100%;

FIG. 2 is a graph showing the amounts of mitochondria measured in examples 1 to 3, comparative example 1 and a control group. (a) Is the amount of mitochondria in cells cultured for 7 days, (b) is the amount of mitochondria in cells cultured for 14 days, and (c) is the amount of mitochondria in cells cultured for 21 days. The amount of mitochondria calculated in the control group was defined as 100%, and the amounts of mitochondria of examples 1 to 3 and comparative example 1 were expressed as a ratio to the amount of mitochondria in the control group;

FIG. 3 is a graph showing the workload imposed on a given amount of mitochondria as measured in examples 1 to 3, comparative example 1, and control. (a) The amount of work carried by a certain amount of mitochondria in cells cultured for 7 days, (b) the amount of work carried by a certain amount of mitochondria in cells cultured for 14 days, and (c) the amount of work carried by a certain amount of mitochondria in cells cultured for 21 days. The amount of work carried by a certain amount of mitochondria calculated in the control group was defined as 100%, and the amount of work carried by a certain amount of mitochondria in examples 1 to 3 and comparative example 1 was represented as a ratio to the amount of work carried by a certain amount of mitochondria in the control group;

FIG. 4 is a graph showing the amounts of apoptotic cells measured in examples 1 to 3, comparative example 1 and control group. (a) Is the amount of apoptotic cells in cells cultured for 7 days, (b) is the amount of apoptotic cells in cells cultured for 14 days, and (c) is the amount of apoptotic cells in cells cultured for 21 days. The amount of apoptotic cells calculated in the control group was defined as 100%, and the amount of apoptotic cells in examples 1 to 3 and comparative example 1 was represented as a ratio to the amount of apoptotic cells in the control group;

FIG. 5 is a graph showing the amounts of necrotic cells measured in examples 1 to 3, comparative example 1, and control group. (a) Is the amount of necrotic cells in cells cultured for 7 days, (b) is the amount of necrotic cells in cells cultured for 14 days, and (c) is the amount of necrotic cells in cells cultured for 21 days. The amount of necrotic cells calculated in the control group was defined as 100%, and the amount of necrotic cells in examples 1 to 3 and comparative example 1 was expressed as a ratio to the amount of necrotic cells in the control group.

Detailed Description

Hereinafter, the method for producing the anti-aging health food and cosmetic of the present invention and the anti-aging component derived from grape seeds will be described.

In the present specification, "senescence" means a state in which the cell multiplication ability is reduced or a state in which the cell growth is irreversibly stopped in G1. These conditions involve inhibition of prohibitionsGenes that progress into the cell cycle, p16 that inhibit the cell cycle^INK4aAnd expression of p53 and the transcription factor p21 as its target^CIP1Senescent cells characterize the phenomenon of senescence in cells with, for example, the activity of senescence-associated acid β -galactosidase (SA β Gal; sensor associated β -galactosidase) as a biochemical marker and have been shown to be useful for characterizing similar phenomena in organisms.

The anti-aging health food and cosmetic of the present invention contains a crude purified anti-aging ingredient derived from grape seeds, which contains more than 60% by weight of polyphenols derived from grape seeds.

The antiaging component containing a polyphenol component derived from grape seeds contained in the antiaging health foods and cosmetics of the invention is obtained by the following production method.

< step 1>

Step 1 is a step of pretreating grape seeds and drying the grape seeds in a pregermination state.

The kind of grape to which the grape seed used in the present invention belongs is not particularly limited, and for example, at least one grape may be selected from european grape (Vitis vinifera L.), american grape (Vitis labrusca L.), ziggu grape (Vitis coignetia L.), Vitis amurensis L, and white congress grape (Vitis shiragai L).

Among them, preferred are Abetique (Agiorigitiko), Vioni (Viogenier), Cabernet Sauvignon (Cabernet France), Pinctare (Camay), Jialian (Carignan), Jiamene (Carmenere), Heixinuo (Xinomaro), Geena (Grenache), Qiongyao slurry (Gewurztraminer), Kerner (Kerner), Columbard (Colombard), Jiazhou, Sudana (Sultana), Mulvesse (Gigiese), Sandonnay (Chardonnay), white poem (Chennin Blanc), Sira (Syrah), Xianpinpink (Zinfandel), Sarron (Selon), Abrus (Black), Pinolo (Pinile), Pinile (Pinilla), Pinilla (Pinilla), Pinolo (Pinolo), Pinilla (Pinilla), Pinolo (Pinolo), Pinolo (Pirolo), Pirolo (Pirolo), and Pinolo (Piraco), Piraco (Sulta), Sulta (Sulta, Bailey a musk (MuscatBailey a), marbeck (Malbec), Muller-chart high (Muller-Thurgau), mujwitt (mourverde), mennier (meinier), burgundy (melons de Bourgogne), Merlot (Merlot), moscator (moscator), sauvignon rubra, renling (rieslling) and Ruby Cabernet, among others, species of vintage grapes, particularly preferred sauvignon rubra, mello, cila, melanopino.

In the present specification, "pregermination" means that grape seeds enter a state in which germination starts. More specifically, it refers to a state in which the germ portion of grape seeds is slightly swollen and swollen.

The degree of the protrusion is not particularly limited, and is, for example, a state of being protruded 1mm to 2mm from the surface of the embryo part of the grape seed before pregermination.

Furthermore, in the present invention, it is not recommended to select grape seeds in a germinated state as a raw material because grape seeds in a germinated state have a lower anti-aging effect than grape seeds in a state to be germinated.

In the present invention, the method of pretreating grape seeds to bring the grape seeds into a sprouting state is not particularly limited, and physical methods such as low-temperature treatment, heat bath treatment, mechanical destruction and the like, chemical methods such as gas (acetylene, ether, hydrogen and the like) treatment, auxin treatment, gibberellin treatment and the like can be selected, and any other conventional method for bringing plant seeds into a sprouting state can also be used.

As described above, in the present invention, the pretreatment method for bringing the grape seeds into the sprouting state is not particularly limited, and for example, the grape seeds can be brought into the sprouting state by the following more specific pretreatment method.

Firstly, grape seeds are immersed in water at 30-60 ℃.

The soaking time is not limited, but is preferably 20 to 80 hours.

And then, fishing out the grape seeds soaked in the water with the temperature of 30-60 ℃ and naturally drying in the air.

The temperature for natural drying is not limited, but is preferably 10 to 50 ℃.

The time for natural drying is not limited, but is preferably 1 to 10 hours.

And then, immersing the grape seeds naturally dried in the air into water at 15-45 ℃.

The soaking time is not limited, but is preferably 10 to 200 minutes.

And then, fishing out the grape seeds soaked in the water at the temperature of 15-45 ℃ and naturally drying in the air.

The temperature for natural drying is not limited, but is preferably 10 to 50 ℃.

The time for natural drying is not limited, but is preferably 3 to 12 hours.

Repeating the step of immersing the grape seeds in water at 15-45 ℃ and the step of naturally drying the grape seeds in the air, and keeping the grape seeds in a state of intermittent moisture content until the germ part of the grape seeds slightly swells and swells after natural drying.

And (3) after the grape seeds are in a state of natural drying and the germ parts of the grape seeds are slightly raised and expanded, placing the grape seeds at a temperature (below 80 ℃) which is enough to kill various bacteria for 2-5 days, and further drying the grape seeds.

In addition, the drying time may be appropriately changed according to the season, the ambient temperature, and the humidity.

After the germination stimulation treatment, phenolic molecules in grape seeds can be peeled off from the thin phenolic layer and polymerized to generate a plurality of polyphenols.

Resveratrol is a polyphenol with a molecular weight of about 250, but the newly formed component is characterized by a large polymer structure and a molecular weight of 4000.

< step 2>

Step 2 is a step of powdering the grape seeds in the pregerminated state dried in step 1.

The powdering method is not limited, and the powdering can be carried out by a conventional method, for example, by a mill or the like.

< step 3>

Step 3 is a step of extracting the powdered grape seeds with a solvent.

By this step 3, the grape seed antiaging component composed of polyphenols derived from grape seeds is obtained.

As the solvent, water, ethanol, or a mixed solvent of water and ethanol can be used.

The extraction can be performed by adding 50 to 1000 parts by weight of a solvent to 100 parts by weight of the grape seed powder.

As described above, the grape seed anti-aging component comprising polyphenols derived from grape seeds comprises a polymer component having a molecular weight of about 4000(3500 to 4500).

< step 4>

Next, the grape seed antiaging component composed of polyphenols derived from grape seeds obtained in step 3 is dried and then powdered.

The drying method is not limited, and drying under reduced pressure is preferably used.

The powdering method is not limited, and conventional methods such as powdering with a mill can be used.

Through the above steps, a powder of the anti-aging component of grape seeds consisting of polyphenols derived from grape seeds can be obtained.

The anti-aging component derived from grape seeds obtained by the above method is subjected to crude purification, and contains 50 to 80 wt% of polyphenol.

In order to achieve an excellent anti-aging effect, the anti-aging ingredient derived from grape seeds is preferably a crude purified polyphenol containing 60% by weight or more.

The polyphenol contained in the antiaging component derived from grape seeds is a polyphenol contained in various grape seeds, for example, resveratrol, tannin, etc.

The proanthocyanidin polymer is contained in the polyphenol contained in the anti-aging component derived from grape seeds in an amount of 50 to 80 wt%.

The present inventors confirmed that an anti-aging ingredient derived from grape seeds containing the above ingredients has an anti-aging effect.

In the present specification, the term "crude purification" means a state in which impurities are contained only by extraction, drying and powdering, and processing such as concentration is not performed. The anti-aging component derived from grape seeds obtained by the steps 1-4 is a crude pure extract.

The anti-aging health food of the present invention can be obtained by adding an anti-aging ingredient derived from grape seeds to a health food.

By adding an anti-aging ingredient derived from grape seeds to a health food, the anti-aging ingredient derived from grape seeds can be easily taken daily.

The anti-aging health food of the present invention may be prepared by appropriately compounding and using additives used in common food materials, for example, glucose, fructose, sucrose, maltose, sorbitol, stevioside, rubusoside, corn syrup, lactose, citric acid, tartaric acid, malic acid, succinic acid, lactic acid, L-ascorbic acid, dl- α -tocopherol, sodium erythorbate, glycerin, propylene glycol, glycerin fatty acid ester, polyglycerin fatty acid ester, sucrose fatty acid ester, sorbitan fatty acid ester, propylene glycol fatty acid ester gum arabic, carrageenan, casein, gelatin, pectin, agar, vitamin B, nicotinamide, calcium pantothenate, amino acids, calcium salts, pigments, flavors, preservatives, and the like, according to a conventional method.

The cosmetic of the present invention can be obtained by adding an anti-aging ingredient derived from grape seeds to the cosmetic.

The anti-aging ingredient derived from grape seeds can be used daily by adding the anti-aging ingredient derived from grape seeds to the cosmetic.

The form of the anti-aging component derived from grape seeds contained in the anti-aging cosmetic of the present invention is not particularly limited, and a multilayered microcapsule is preferable in order to allow easier penetration into the skin.

The anti-aging cosmetic can be properly matched with common additives such as a whitening agent, an active oxygen remover, an antioxidant, an ultraviolet ray preventer and the like and prepared according to a conventional method.

Although the intake amount of the anti-aging component derived from grape seeds in the anti-aging health food and the anti-aging cosmetic of the present invention is not limited, it is preferable that the intake amount per day is at least 50mg or more in order to achieve an excellent anti-aging effect.

If the daily intake of the anti-aging component derived from grape seeds in the anti-aging health food and the anti-aging cosmetic of the present invention is less than 50mg, the anti-aging effect may not be sufficiently exerted, and thus it is not desirable.

In addition, the anti-aging ingredient derived from grape seeds in the anti-aging health food and the anti-aging cosmetic of the present invention has little gain in effect even if it is ingested in excess of 7500mg (body weight 60 kg) per day.

Therefore, the anti-aging ingredient derived from grape seeds in the anti-aging health food and the anti-aging cosmetic of the present invention is preferably ingested at 50mg to 7500mg per day.

In addition, the anti-aging health food and the anti-aging cosmetic of the present invention may be appropriately compounded with excipients, pH regulators, preservatives, chelating agents, stabilizers and the like according to the intended use.

In addition, although the intake amount per kg body weight of the anti-aging component derived from grape seeds contained in the anti-aging health food and the anti-aging cosmetic of the present invention is not limited, for example, the intake amount is desirably 0.8 to 125 mg/kg/day in order to achieve an excellent anti-aging effect.

If the intake amount of the anti-aging component derived from grape seeds in the anti-aging health food and the anti-aging cosmetic of the present invention is less than 0.8 mg/kg/day per kg of body weight, the anti-aging effect may not be sufficiently exerted, and thus it is not preferable.

In addition, the anti-aging ingredient derived from grape seeds in the anti-aging health food and the anti-aging cosmetic of the present invention has no change in effect even if ingested in excess of 125 mg/kg/day.

Therefore, the preferable intake amount of the anti-aging component derived from grape seeds in the anti-aging health food and the anti-aging cosmetic of the present invention is 0.8 to 125 mg/kg/day.