阅读说明：本技术 一种治疗骨折、关节炎及急慢性软组织损伤的中药组合物及其制备方法 (A Chinese medicinal composition for treating fracture, arthritis and acute and chronic soft tissue injury, and its preparation method ) 是由 段建西 段景怡 于 2020-06-23 设计创作，主要内容包括：本发明公开了一种治疗骨折、关节炎及急慢性软组织损伤的中药组合物及其制备方法,其特征在于,它是由有效成分和辅料组成,所述有效成分为：川续断10-50g、骨碎补10-50g、巴吉天10-50g、透骨草5-40g、乳香5-30g、没药5-40g、自然铜10-50g、川牛膝5-40g、土鳖虫5-30g、当归5-40g、红花5-40g、龙骨10-55g、蜂蜜400-600g。本发明中药组合物可直接作用于患处,增加局部血流速度和血管通透性,使病灶周围血管扩张,血流加速,同时促进药物有效成分的渗入吸收,以促进炎症吸收和肿胀的消退,加快局部血液循环,缩短治疗时间,促进骨折快速康复。(The invention discloses a Chinese medicinal composition for treating fracture, arthritis and acute and chronic soft tissue injury and a preparation method thereof, and is characterized in that the Chinese medicinal composition comprises effective components and auxiliary materials, wherein the effective components comprise: 10-50g of dipsacus asperoides, 10-50g of drynaria rhizome, 10-50g of radix angelicae batryae, 5-40g of garden balsam stem, 5-30g of frankincense, 5-40g of myrrh, 10-50g of native copper, 5-40g of medicinal cyathula root, 5-30g of ground beetle, 5-40g of angelica, 5-40g of safflower, 10-55g of keel and 600g of honey 400-. The Chinese medicinal composition can directly act on an affected part, increase the local blood flow speed and the vascular permeability, dilate blood vessels around a focus, accelerate blood flow, promote the infiltration and absorption of effective components of the medicament, promote the absorption of inflammation and the regression of swelling, accelerate the local blood circulation, shorten the treatment time and promote the quick recovery of fracture.)

1. A Chinese medicinal composition for treating fracture, arthritis and acute and chronic soft tissue injury is characterized by comprising effective components and auxiliary materials, wherein the effective components are as follows: 10-50g of dipsacus asperoides, 10-50g of drynaria rhizome, 10-50g of radix angelicae batryae, 5-40g of garden balsam stem, 5-30g of frankincense, 5-40g of myrrh, 10-50g of native copper, 5-40g of medicinal cyathula root, 5-30g of ground beetle, 5-40g of angelica, 5-40g of safflower, 10-55g of keel and 600g of honey 400-.

2. The traditional Chinese medicine composition of claim 1, wherein the effective components further comprise 5-40g of rheum officinale and 5-25g of mirabilite.

3. The traditional Chinese medicine composition of claim 1, wherein the effective ingredients further comprise 5-30g of cuttlebone and 10-40g of endothelium corneum gigeriae galli.

4. The traditional Chinese medicine composition of claim 2 or 3, wherein the effective ingredients are: 15-45g of dipsacus asperoides, 15-45g of drynaria rhizome, 15-50g of radix angelicae gigantis, 10-30g of garden balsam stem, 10-30g of frankincense, 10-35g of myrrh, 10-40g of native copper, 5-30g of medicinal cyathula root, 5-30g of ground beetle, 5-40g of angelica, 5-40g of safflower, 10-50g of dragon bone, 5-40g of rhubarb, 5-25g of mirabilite, 10-30g of cuttlebone, 10-40g of chicken's gizzard-membrane and 600g of honey 400.

5. The traditional Chinese medicine composition of claim 4, wherein the effective ingredients are: 15-45g of dipsacus asperoides, 15-45g of drynaria rhizome, 15-50g of radix angelicae gigantis, 10-30g of garden balsam stem, 10-30g of frankincense, 10-30g of myrrh, 20-40g of native copper, 10-30g of medicinal cyathula root, 10-30g of ground beetle, 10-30g of angelica, 5-40g of safflower, 22-46g of dragon bone, 10-25g of rhubarb, 10-20g of mirabilite, 20-30g of cuttlebone, 20-30g of endothelium corneum gigeriae galli and 500g of honey.

6. The traditional Chinese medicine composition of claim 5, wherein the effective ingredients are: 30g of dipsacus asperoides, 30g of drynaria rhizome, 30g of radix baji, 20g of garden balsam stem, 20g of frankincense, 20g of myrrh, 30g of native copper, 20g of medicinal cyathula root, 20g of ground beetle, 20g of angelica, 20g of safflower, 36g of dragon bone, 20g of rhubarb, 15g of mirabilite, 20g of cuttlebone, 30g of endothelium corneum gigeriae galli and 500g of honey.

7. The Chinese medicinal composition according to any one of claims 1 to 6, wherein the composition is added with pharmaceutically acceptable excipients commonly used in pharmacy to prepare an external patch.

8. A method of preparing the traditional Chinese medicine composition of claim 1, 2 or 3, characterized in that:

[1] respectively drying and crushing the 16 raw material medicines, and sieving the raw material medicines by a 100-mesh sieve for later use;

[2] mixing 500kg of honey with 80ml of azone, adding the medicinal powder prepared in the step, fully stirring and uniformly mixing to prepare paste, coating the paste on auxiliary materials, and adding the paste to a non-woven fabric adhesive plaster to obtain the honey-fried glutinous rice flour.

9. The use of the Chinese medicinal composition of claim 1, 2 or 3 in the preparation of medicaments for treating bone fracture, arthritis, acute and chronic soft tissue injury, etc.

Technical Field

The invention belongs to the field of traditional Chinese medicines, and particularly relates to a traditional Chinese medicine composition for treating fracture, arthritis and acute and chronic soft tissue injury and a preparation method thereof.

Background

Fracture is a common clinical orthopedic disease, and refers to the condition that the bone structure is completely or partially broken continuously. The causes of fractures are mainly related to direct or indirect violence, long-term or repeated injury and other factors. In recent years, the incidence of fracture in China is gradually increased, and clinical symptoms of patients after fracture are mainly manifested as limited movement, deformity, grating bone and the like. Fracture can seriously damage the motor function of a patient, so that the life quality of the patient is linearly reduced, and the patient needs to be treated in time.

At present, the main clinical treatment means for fracture include operations, plaster fixation, brace fixation and the like.

After fracture, local tissues can be seriously damaged, pain and swelling occur, and local bleeding occurs, the operation is a wound, the pain and swelling are easily aggravated, adverse effects are caused on wound healing of a patient, nerve damage, blood vessel damage, venous reflux obstruction and the like are easily caused by poor treatment, the injured parts are accompanied with the pain and swelling for a long time, even osteofascial compartment syndrome is caused, wound healing is delayed, and postoperative rehabilitation and life quality of the patient are seriously affected; and the operation treatment fracture aggravates the time of the patient lying in bed, the treatment period, the cost is high, and some operations even need to be repeated for a plurality of times, thereby bringing the patient with the double-body fracture.

The plaster and the brace are adopted for fixing and treating the fracture, the venous and lymphatic return is blocked due to long-time fixation, the phenomena of muscular atrophy, blood vessel nerve regulation dysfunction and the like are caused, the function of a deep venous valve of the lower limb is reduced, the tissue edema is caused, the delay rehabilitation is mainly treated clinically by adopting conventional means of diminishing inflammation, relieving pain, stopping bleeding and the like at present, and the aim is to promote the rapid healing of the fracture, but the effect is poor.

In clinic, although the patient is treated by western medicine timely and effectively, the best rehabilitation therapy opportunity is missed due to swelling, pain and the like. The traditional Chinese medicine has certain help in relieving fracture pain and swelling, provides good conditions for carrying out rehabilitation treatment as early as possible, and is beneficial to fracture healing. The healing and blood circulation promoting decoction is used for treating the initial stage of fracture, and aims to relieve pain, accelerate the subsidence of swelling, promote the blood circulation around the fracture and create good conditions for the early development of rehabilitation training.

The traditional Chinese medicine has great advantages in the aspect of preventing and treating osteoarthritis, and the traditional Chinese medicine theory holds that meridians and collaterals are damaged after the body is fractured, so that nutrient blood is separated from meridians and collaterals, the veins are blocked, and the nutrient blood is accumulated in skin striae, and further swelling is caused. The blood stasis of the meridians and qi stagnation can lead to venous obstruction and pain, which can cause swelling and pain. Therefore, the treatment principle is that the swelling and pain can be relieved, the blood circulation can be promoted, the blood stasis can be removed, and the nutrient-blood can be separated from the meridians and can be blocked and accumulated in the skin striae, thereby causing the swelling. The blood stasis of the meridians and qi stagnation can lead to venous obstruction and pain, which can cause swelling and pain. Therefore, the treatment principle is mainly to relieve swelling and pain, activate blood and resolve stasis, and clear and activate the channels and collaterals. The stasis can not be removed when blood is not alive, the bone can not be connected when the blood stasis is not removed, the traditional Chinese medicine for reuniting the bones and the tendons has the functions of promoting the absorption of the blood stasis, the swelling subsides, the circulation of qi and blood and accelerating the growth of callus, the external application traditional Chinese medicine has higher application value in the treatment of fracture patients, the external application traditional Chinese medicine can directly act on the affected part by contacting the affected part, thereby increasing the speed of local blood flow and the permeability of blood vessels, expanding the blood vessels around the focus, accelerating the blood flow, simultaneously promoting the infiltration and absorption of effective components of the medicine, promoting the absorption of inflammation and the subsidence of the swelling, accelerating the local blood circulation, shortening the treatment time, promoting the quick recovery of the fracture, improving the.

Disclosure of Invention

The invention aims to provide a traditional Chinese medicine composition for treating fracture, arthritis and acute and chronic soft tissue injuries and a preparation method thereof.

In order to achieve the purpose, the technical scheme adopted by the invention comprises the following steps:

a Chinese medicinal composition for treating fracture, arthritis and acute and chronic soft tissue injury is characterized by comprising effective components and auxiliary materials, wherein the effective components are as follows: 10-50g of dipsacus asperoides, 10-50g of drynaria rhizome, 10-50g of radix angelicae batryae, 5-40g of garden balsam stem, 5-30g of frankincense, 5-40g of myrrh, 10-50g of native copper, 5-40g of medicinal cyathula root, 5-30g of ground beetle, 5-40g of angelica, 5-40g of safflower, 10-55g of keel and 600g of honey 400-.

More preferably, the effective components also comprise 5-40g of rhubarb and 5-25g of mirabilite.

Preferably, the effective components also comprise 5-30g of cuttlebone and 10-40g of chicken's gizzard-membrane.

Further preferably, the effective components in the traditional Chinese medicine composition are as follows: 15-45g of dipsacus asperoides, 15-45g of drynaria rhizome, 15-50g of radix angelicae gigantis, 10-30g of garden balsam stem, 10-30g of frankincense, 10-35g of myrrh, 10-40g of native copper, 5-30g of medicinal cyathula root, 5-30g of ground beetle, 5-40g of angelica, 5-40g of safflower, 10-50g of dragon bone, 5-40g of rhubarb, 5-25g of mirabilite, 10-30g of cuttlebone, 10-40g of chicken's gizzard-membrane and 600g of honey 400.

Further preferably, the effective components in the traditional Chinese medicine composition are as follows: 15-45g of dipsacus asperoides, 15-45g of drynaria rhizome, 15-50g of radix angelicae gigantis, 10-30g of garden balsam stem, 10-30g of frankincense, 10-30g of myrrh, 20-40g of native copper, 10-30g of medicinal cyathula root, 10-30g of ground beetle, 10-30g of angelica, 5-40g of safflower, 22-46g of dragon bone, 10-25g of rhubarb, 10-20g of mirabilite, 20-30g of cuttlebone, 20-30g of endothelium corneum gigeriae galli and 500g of honey.

Further preferably, the effective components in the traditional Chinese medicine composition are as follows: 30g of dipsacus asperoides, 30g of drynaria rhizome, 30g of radix baji, 20g of garden balsam stem, 20g of frankincense, 20g of myrrh, 30g of native copper, 20g of medicinal cyathula root, 20g of ground beetle, 20g of angelica, 20g of safflower, 36g of dragon bone, 20g of rhubarb, 15g of mirabilite, 20g of cuttlebone, 30g of endothelium corneum gigeriae galli and 500g of honey.

Furthermore, the traditional Chinese medicine composition is added with pharmaceutical auxiliary materials commonly used in pharmaceutics to prepare an external patch.

The invention also aims to provide a preparation method of the traditional Chinese medicine composition for treating fracture, arthritis and acute and chronic soft tissue injury, which is characterized by comprising the following steps:

[1] respectively drying and crushing the raw material medicines, and sieving the raw material medicines by a 100-mesh sieve for later use;

[2] mixing 500kg of honey with 80ml of azone, adding the medicinal powder prepared in the step, fully stirring and uniformly mixing to prepare paste, coating the paste on auxiliary materials, and adding the paste to a non-woven fabric adhesive plaster to obtain the honey-fried glutinous rice flour.

Furthermore, the invention also aims to protect the application of the traditional Chinese medicine composition in preparing medicines for treating fracture, arthritis, acute and chronic soft tissue injury and the like.

The traditional Chinese medicine comprises the following traditional Chinese medicinal materials in the formula:

keel: sweet, astringent and slightly cold; the functional indications are as follows: calming the liver and suppressing yang, tranquilizing and allaying excitement, astringing and arresting discharge, yin deficiency and yang hyperactivity, dysphoria and irascibility, dizziness, blurred vision, uneasiness, palpitation, insomnia, epilepsy, mania, spermatorrhea, leukorrhagia, sweating, metrorrhagia.

Rhubarb: bitter and cold; the functional indications are as follows: it has effects in clearing away heat and toxic materials, purging pathogenic accumulation, clearing away heat, purging pathogenic fire, promoting blood circulation, and dispelling blood stasis, and can be used for treating intestinal stagnation, constipation, hematemesis due to blood heat, epistaxis due to hemorrhage, conjunctival congestion, pharyngalgia, gingival swelling and pain, burn, female blood stasis, amenorrhea, puerperal lochiorrhea, scar accumulation, and traumatic injury.

Mirabilite: salty, bitter and cold; the functional indications are as follows: purgation, hardness softening and heat clearing, and can be used for treating excess heat accumulation, constipation, pharyngalgia, aphtha, conjunctival congestion and pyocutaneous disease.

Cuttlebone: salty, astringent and slightly warm; the functional indications are as follows: metrorrhagia, metrostaxis, hemorrhage of lung and stomach, hemorrhage due to trauma, spermatorrhea, leukorrhagia, gastralgia, acid regurgitation, eczema, and ulcer with pus.

Endothelium corneum gigeriae galli: sweet and mild; the functional indications are as follows: activating spleen to promote digestion, and securing essence to stop enuresis, and originally used for treating dyspepsia, indigestion, cellulitis in the interior of children and the like.

Teasel root: bitter, sweet, pungent and slightly warm; the functional indications are as follows: tonify liver and kidney, promote blood circulation, reunite tendons and bones, and treat lumbago, leg weakness, spermatorrhea, metrorrhagia, traumatic injury, incised wound, carbuncle, ulcer.

Rhizoma drynariae: bitter and warm; the functional indications are as follows: invigorating kidney, promoting blood circulation, stopping bleeding, and healing wound, and can be used for treating lumbago due to kidney deficiency, foot weakness, tinnitus, deafness, toothache, chronic diarrhea, traumatic injury, contusion, and injury of bones and muscles.

Baji day: pungent, sweet and slightly cold; the functional indications are as follows: invigorating kidney, tonifying yang, dispelling pathogenic wind, removing dampness, and can be used for treating sexual impotence, frequent micturition, infertility due to cold womb, menoxenia, lower abdomen psychroalgia, lumbago, gonalgia, and weakness.

Garden balsam stem: pungent and warm; the functional indications are as follows: promoting blood circulation and relieving pain, and can be used for treating rheumatic arthritis, spasm of tendons and bones, eczema, pyocutaneous disease, toxic swelling, and numbness of limbs.

Frankincense: pungent, bitter and slightly warm; the functional indications are as follows: promoting blood circulation and activating qi-flowing, inducing menstruation to relieve pain, subsiding swelling and promoting granulation, relieving pain of heart and abdomen, rheumatalgia, amenorrhea and dysmenorrhea, traumatic injury and blood stasis and pain, superficial infection and toxic swelling, appendicitis, and unhealed sore and ulcer.

Myrrh: bitter and flat; the functional indications are as follows: promoting blood circulation, relieving pain, subsiding swelling, promoting granulation, blood stasis and pain in chest and abdomen, dysmenorrhea, amenorrhea, abdominal mass, traumatic injury, carbuncle, swelling, sore and ulcer, intestinal abscess, and conjunctival congestion with swelling and pain.

Native copper: pungent and mild; the functional indications are as follows: removing blood stasis, relieving pain, setting bone and healing wound, and can be used for treating traumatic injury, fracture, and swelling and pain due to blood stasis.

Radix cyathulae: bitter, sour and mild; the functional indications are as follows: promoting blood circulation, dispelling blood stasis, nourishing liver and kidney, strengthening tendons and bones, inducing diuresis, treating stranguria, and promoting blood circulation, and can be used for treating menoxenia due to blood stasis, dysmenorrhea, amenorrhea, puerperal abdominal pain due to blood stasis, traumatic injury, soreness of waist and knees, weakness of lower limbs, hematuria, dysuria, and urethral pain.

Ground beetle: salty, cold and slightly toxic; the functional indications are as follows: removing blood stasis, and promoting reunion of fractured tendons and bones, and can be used for treating amenorrhea, puerperal stagnation, abdominal mass, fracture injury, stagnation pain, and lumbar sprain.

Chinese angelica: sweet, pungent and warm, with the main functions: replenishing blood, promoting blood circulation, relieving pain, and moistening intestine, and can be used for treating blood deficiency syndrome, menoxenia, amenorrhea, dysmenorrhea, asthenia cold abdominal pain, blood stasis pain, traumatic injury, arthralgia, numbness, superficial infection, and pyocutaneous disease.

Safflower: pungent and warm; the functional indications are as follows: promoting blood circulation, dispelling blood stasis, dredging channels, promoting blood circulation, amenorrhea, puerperal stagnation, abdominal pain, abdominal mass, traumatic injury, blood stasis and pain, and arthralgia.

Honey: sweet and mild; the functional indications are as follows: lubricant and excipient, can be administered orally for invigorating middle warmer, relieving spasm, moistening lung, relieving cough, and relaxing bowels, and can be used as ointment and plaster matrix for external use.

The prescription is reasonable and innovative, radix cyathulae and teasel root in the prescription have obvious curative effects of tonifying liver and kidney and strengthening bones and muscles, especially in osteoporosis and fracture, the radix stemonae and the rhizoma drynariae can reunite bones and tendons, dissipate blood stasis and relieve pain, the broken bones can promote the absorption of the bones to calcium, the levels of blood calcium and blood phosphorus are improved, the fracture healing is facilitated, the garden balsam stems can relax tendons and activate collaterals, the frankincense and the myrrh can promote blood circulation and relieve pain, the blood viscosity can be reduced, the microcirculation is improved, the platelet aggregation and the thrombus formation are inhibited, the absorption and the blood circulation of blood stasis can be promoted, conditions are created for organism tissue repair, the safflower is used for removing blood stasis and relieving pain, the carthamin the safflower can effectively reduce the platelet aggregation and inhibit the formation, the angelica is used for promoting blood circulation and removing blood stasis, the platelet aggregation can be reduced, the immune function can also be adjusted, and the native copper.

Mirabilite is a crystal refined from natural minerals containing sodium sulfate, and has the main effects of purgation, hardness softening and heat clearing. The rhubarb has the main functions of purgation and accumulation elimination, heat clearing and fire purging, hemostasis, detoxification, blood circulation activation and blood stasis removal, and the mirabilite and the rhubarb are used together, so that the constipation is treated, the fat liquefaction, infection and hematoma of an incision in an abdominal operation can be prevented and treated, the healing time of the incision can be shortened, and the treatment effects mainly come from the anti-inflammation, swelling-diminishing and pain-relieving effects of the rhubarb and the mirabilite.

Cuttlebone, with the actions of relieving hyperacidity and pain, astringing and arresting seminal emission, blood and leucorrhea, is currently used in the treatment of various bleeding, gastroduodenal ulcer, chronic gastritis and other diseases. But the book of Yi Xue Zhong xi Lu considers that it has the functions of tonifying kidney meridian and assisting kidney to store, besides it is salty in taste and warm in nature, so Hai Piao Xiao can also warm and remove blood stasis and blood, and has mild potency of medicine, and can promote the formation of blood vessels at the fracture part to different degrees.

The endothelium corneum gigeriae galli has the effects of helping digestion and strengthening spleen, arresting seminal emission and arresting enuresis, treating stranguria and dissolving calculus and regulating blood rheology, is commonly used for diseases such as indigestion, infantile malnutrition, enuresis and nocturnal emission, stranguria with stone and relieving pain, and is considered by the records of Zhongzhong Zhongxi of medicine to eliminate accumulation of spleen and stomach and any accumulation syndrome, and blood stasis in blood vessels of muscles and bones is actually accumulation of blood stasis, and the endothelium gigeriae galli contains abundant gastric hormone, amylase, trace pepsin, keratin and various amino acids, and can permeate skin to promote healing of local damaged tissues when being externally used. Although the two medicines of the cuttlebone and the endothelium corneum gigeriae galli are both rarely applied to the fracture, the clinical practice proves that the cuttlebone is compatible with the endothelium corneum gigeriae galli, the two medicines take the effects of dispersing blood stasis together and have mild medicine properties, and the effect of treating the bone fracture disease is clear.

The honey is sweet and nontoxic, has the effects of tonifying qi and strengthening the middle-jiao, relieving pain and detoxifying, and removing diseases, and contains acidic substances and high-concentration carbohydrate substances, so that bacteria can hardly survive at wound parts, the infection caused by blood stasis is reduced, and the absorption caused by local blood stasis is facilitated. Meanwhile, the original pure honey contains biotin which plays a promoting role in organism generation, so the pure honey is selected as a substrate. The medicines are used together to play the functions of relieving swelling and pain, promoting reunion of fractured bones, and clearing and activating the channels and collaterals.

Compared with the prior art, the traditional Chinese medicine composition disclosed by the invention is reasonable and innovative in formula, has the effects of promoting blood circulation to remove blood stasis, relieving swelling and eliminating swelling, warming and activating meridians, strengthening muscles and bones, eliminating dampness and activating collaterals, and warming and relieving pain, can exert good effects of dredging meridians, activating blood circulation to remove blood stasis, accelerating the healing of fracture, promoting the subsidence of swelling, accelerating local blood circulation, and effectively avoiding the occurrence of complications such as joint stiffness, muscular atrophy and the like.

The traditional Chinese medicine composition can treat various arthritis, osteoarthropathy and gouty arthritis caused by wind-cold-dampness blocking channels and collaterals; muscle and bone pain, debilitation and cramp caused by chronic strain. The traditional Chinese medicine has mild nature and taste, does not cause obvious adverse reaction and toxic and side effect to patients, has low price, is easy to master a treatment method, does not cause great psychological pressure and economic burden to the patients, and has relatively high acceptance and tolerance of the patients.

Detailed Description

The present invention will be further described with reference to the following examples, but the present invention is not limited thereto.

The following is a pharmacodynamic study of the composition of the present invention, and in order to simplify the experimental operation and save the research cost, and follow the experimental principles of parallel control, thereby more scientifically highlighting the creativity of the technical solution of the present invention, we will typically perform a representative study on one technical solution group of the present invention, which can be fully deduced by those skilled in the art to understand the beneficial effects of other parameter point technical solutions in the present application, and therefore the technical content and the pharmacodynamic results of the present invention are by no means limited to this scope.

Application embodiment 1

1 test materials and results processing method:

1.1 preparation of the test drugs of the invention:

[1] the raw material medicaments in the following weight ratio are taken: 30g of dipsacus asperoides, 30g of drynaria rhizome, 30g of radix baji, 20g of garden balsam stem, 20g of frankincense, 20g of myrrh, 30g of native copper, 20g of medicinal cyathula root, 20g of ground beetle, 20g of angelica, 20g of safflower, 36g of dragon bone, 20g of rhubarb, 15g of mirabilite, 20g of cuttlebone and 30g of endothelium corneum gigeriae galli. Drying and crushing, and sieving with a 100-mesh sieve for later use;

[2] adding 500g of honey into the medicinal powder prepared in the step, fully stirring and uniformly mixing, and uniformly coating the medicinal powder and auxiliary materials to obtain the honey-honey.

1.2 test animals: SD mice and rats provided by the Experimental animals center of Shanxi university of traditional Chinese medicine;

1.3 statistical treatment: results of the experiment toIndicating that the significance of the difference between groups was determined using the t-test.

2. Pharmacodynamic test and results:

2.1 anti-inflammatory assay:

effect on swelling of mouse ear: 20 mice are taken and randomly divided into 2 groups, administration is carried out once a day, before administration, a depilatory area with the same size is formed on the back of each group of mice, then test medicines are externally pasted and smeared on the area for continuous administration for 7d, after last administration lh, the front and back surfaces of the right ear of each group of mice are coated with 0.05ml of dimethylbenzene, after 2h of dimethylbenzene, the animals are killed, ears are cut, corresponding parts are punched with 8mm steel lugs, the lugs are weighed, and the difference between the weights of the left ear and the right ear is taken as the swelling degree, and the result is shown in table 1.

TABLE 1 Effect of paraxylene on ear swelling in mice

Note: p <0.01 compared to blank group.

The influence on the permeability of the skin capillary vessels of the mice is as follows: 20 mice were divided into 2 groups at random, and once daily administration was carried out, before the administration, a depilated area of uniform size was formed on the back of each group of mice, and then the test drug was applied to the area by external application and smeared for 7 days, and after the last lh administration, l% Evans blue physiological saline was injected into the tail vein at 0.1ml/10g body weight, and histamine was immediately injected into the abdominal depilated area at 5. mu.g (0.1ml) intradermally. After 20min, the animals were sacrificed, blue-stained skin pieces were cut off, soaked in a mixture of acetone physiological saline (7: 3), and after 24min, centrifuged to take the supernatant, and the result was subjected to color comparison at a wavelength of 610nm in a model 721 spectrophotometer, and is shown in Table 2.

TABLE 2 Effect on mouse skin capillary Permeability

Note: p <0.001 compared to blank group.

Effects on rat paw swelling: 20 rats are randomly divided into 2 groups, before administration, the back of each group of mice forms a depilatory area with the same size, test medicines are externally applied and smeared on the area 24h and lh before inflammation respectively, 7d of continuous administration is carried out, 0.1ml of welan gum is injected into 1% of the corner under the tendon membrane of the right and rear foot of the rat to cause foot swelling, the foot volumes before and after modeling (1, 3, 5 and 7h) are measured by a foot volume method, and the difference between the front and the rear is used for representing the foot swelling degree, and the result is shown in table 3.

Table 3 Effect on carrageenan-induced foot swelling in rats (n ═ 10)

Note: p <0.01, P <0.001 compared to blank.

The anti-inflammatory results in tables 1-3 show that the drug group of the present invention has significant differences in the effects on mouse ear swelling, rat foot swelling, and other tests, compared to the blank group.

2.2 analgesic test

The method has the following effects of torsion-induced reaction of mouse acetic acid: 20 mice are taken and randomly divided into 2 groups, administration is carried out once a day, depilatory areas with the same size are formed on the backs of the mice in each group before administration, then test medicines are externally pasted and smeared on the areas for continuous administration for 5 days, 0.6% glacial acetic acid is injected into the abdominal cavity per mouse after last intragastric administration lh, the times of torsion reactions (belly indent, hind limb extension and hip elevation) of the mice within 20min are observed and recorded, and the results are shown in table 4.

TABLE 4 analgesic Effect on mouse acetic acid writhing response

Note: p <0.001 compared to blank group.

The influence on the threshold value of the electric stimulation tail-flicking pain of the mouse is as follows: 20 mice are taken and randomly divided into 2 groups, administration is carried out once a day, before administration, a depilatory area with the same size is formed on the back of each group of mice, then a test medicament is externally pasted and smeared on the area, administration is continuously carried out for 5 days, after last intragastric administration lh, one electrode is placed on the feet of the mice, the other electrode stimulates the tail, after electrification, the pain threshold value of the mice in tail flicking is observed, and the analgesic inhibition rate is calculated, and the result is shown in Table 5.

The analgesic inhibition rate is (pain threshold of administration group-pain threshold of control group)/pain threshold of control group × 100%

TABLE 5 Effect on electrical stimulation tail-flick threshold in mice

Note: p <0.001 compared to blank group.

Effects on mouse hot plate stimulation on the threshold of foot licking: the mouse is placed on a hot plate apparatus (the temperature is 55 ℃), the time required for the mouse to lick the foot after the mouse is placed on the hot plate apparatus is taken as the pain threshold value of the mouse, and the animal which is unqualified is regarded as the animal when the time for licking the foot is less than 5 seconds or more than 30 seconds or jumps. 20 qualified mice were randomly divided into 2 groups, and once a day, before administration, a depilated area of the same size was formed on the back of each group of mice, and then the test drug was applied to the area by external application for three consecutive days, and 1 hour after the last administration, the pain threshold of the mice was measured, and the results are shown in table 6.

TABLE 6 Effect on Hot plate stimulation of mice on the threshold for licking foot pain

Note: p <0.001 compared to blank group;

the results in tables 4-6 show that the drug group of the invention has significant differences in experiments of body writhing with acetic acid, threshold value of tail flicking pain by electrical stimulation and threshold value of foot licking pain by hot plate stimulation compared with the blank control group.

2.3 Effect on the blood System

First, influence on acute blood stasis type white rat blood rheology indexes

20 rats were randomly divided into 2 groups, administered once a day, the backs of the rats in each group were formed into depilatory regions of uniform size before administration, then test drugs were applied to the regions by external application for three consecutive days, the four limbs and the head of the rat were fixed after the last administration for 1 hour, sterilized with 75% alcohol, placed around the experimental rat in an ice bag, frozen for 1 hour, rewarmed for 5min in 45 ℃ warm water, and then raised for 3 days conventionally, blood was collected and the hemorheology was measured, and the results are shown in tables 7 and 8.

TABLE 7 Effect on rat Whole blood viscosity ((S))n＝10)

TABLE 8 Effect on rat hemorheology indices (n＝10)

Note: p <0.01, P <0.001 compared to the blank water group.

The results in tables 7 and 8 show that the drug groups of the present invention have significant differences in whole blood viscosity at high, medium, and low shear rates when compared to the blank control group; in addition, the blood plasma viscosity and the hematocrit are remarkably different, and the results show the great advantages of the medicine group.

Application example two