阅读说明：本技术 一种用于羊栖菜多糖的脱色方法 (Decolorizing method for sargassum fusiforme polysaccharide ) 是由 金旭东 汪财生 丁浩淼 李贵锋 钱国英 王俊琪 李沁清 白雪 陈雪佳 于 2020-04-03 设计创作，主要内容包括：本发明涉及一种用于羊栖菜多糖的脱色方法,包括以下步骤：(1)氧化铝活化,按一定配比将氧化铝、氧化镁以及白陶土混合均匀,将蒸馏水搅拌均匀,装入层析柱,压柱；(2)将待脱色的羊栖菜多糖溶于水中配制成一定浓度的羊栖菜多糖溶液,离心取上清；(3)将上清液过层析柱,洗脱,真空冷冻干燥得脱色后的羊栖菜多糖。与现有技术相比,本发明对羊栖菜多糖的生物活性影响较小,吸附效果好,且脱色效率高,可实现在线洗脱,进而实现连续生产,达到规模化的要求。(The invention relates to a decolorizing method for sargassum fusiforme polysaccharide, which comprises the following steps: (1) activating alumina, uniformly mixing the alumina, the magnesium oxide and kaolin according to a certain ratio, uniformly stirring distilled water, and filling into a chromatographic column and a compression column; (2) dissolving sargassum fusiforme polysaccharide to be decolorized in water to prepare sargassum fusiforme polysaccharide solution with a certain concentration, and centrifuging to obtain supernatant; (3) and (4) passing the supernatant through a chromatographic column, eluting, and carrying out vacuum freeze drying to obtain the decolorized sargassum fusiforme polysaccharide. Compared with the prior art, the method has the advantages of small influence on the biological activity of the sargassum fusiforme polysaccharide, good adsorption effect and high decolorization efficiency, can realize online elution, further realize continuous production and meet the requirement of large scale.)

1. A decolorization method for sargassum fusiforme polysaccharide is characterized by comprising the following steps:

(1) activating alumina, uniformly mixing the alumina, the magnesia and the kaolin according to a certain proportion, uniformly stirring distilled water, filling the mixture into a chromatographic column, and pressing the chromatographic column under a certain pressure to obtain an alumina-magnesia-kaolin composite chromatographic column;

(2) dissolving sargassum fusiforme polysaccharide to be decolorized in water to prepare sargassum fusiforme polysaccharide solution with a certain concentration, and centrifuging to obtain supernatant;

(3) and (3) passing the supernatant obtained in the step (2) through the alumina-magnesia-kaolin composite chromatographic column, eluting, and carrying out vacuum freeze drying to obtain the decolorized sargassum fusiforme polysaccharide.

2. The decoloring method according to claim 1, wherein the alumina, the magnesia and the kaolin in the step (1) are mixed in the following ratio by mass: 1-2: 1-4: 1-2.

3. The decoloring method according to claim 1, wherein the column pressure in the step (1) is 10 to 100 bar.

4. The decoloring method according to claim 1, wherein the concentration of the sargassum fusiforme polysaccharide solution of the step (2) is 1/15 to 1/10.

5. The decoloring method according to any one of claims 1 to 4, wherein the activation of the alumina in the step (1) is: and soaking the alumina in distilled water, naturally settling until the supernatant is not turbid, and drying the precipitate at 200-250 ℃ for 4-12 h.

6. The decolorization method according to any one of claims 1 to 4, wherein a material of the chromatography column in step (1) is a glass or a metal capable of withstanding a pressure of 100 to 150 bar.

7. The decoloring method according to any one of claims 1 to 4, wherein the centrifugation in step (2) is performed at 6000 to 10000rpm for 20 to 40 min.

8. The decolorization method according to any one of claims 1 to 4, wherein the elution conditions in step (3) are: the eluent is distilled water, the elution pressure is 100bar, the elution speed is 2 BV/h-3 BV/h, and the elution volume is 3 BV-5 BV.

9. A decolorization process according to any one of claims 1 to 4, characterized in that the alumina is neutral alumina.

10. A decolorization process according to any one of claims 1 to 4, characterized in that the magnesium oxide is light magnesium oxide.

Technical Field

The invention relates to the technical field of food processing, in particular to a decolorizing method for sargassum fusiforme polysaccharide.

Background

Sargassum fusiforme polysaccharide is an extract of Sargassum fusiforme, and has effects of inhibiting blood cholesterol, triglyceride and low density lipoprotein cholesterol, and increasing high density lipoprotein cholesterol content. The sargassum fusiforme polysaccharide obtained by the traditional extraction method usually contains a large amount of pigment, and the direct use of the sargassum fusiforme polysaccharide as a raw material for producing food or cosmetics can affect the sensory evaluation of products, so that the decolorization treatment is needed. The existing polysaccharide decoloring method comprises activated carbon decoloring, macroporous resin decoloring, hydrogen peroxide decoloring and the like, and the existing polysaccharide decoloring method for the sargassum fusiforme polysaccharide has a plurality of problems, such as: when activated carbon is used for decoloring, the activated carbon needs to be pretreated, and the problems of long decoloring time, repeated decoloring and the like exist; when the hydrogen peroxide is used for decoloring, the biological activity of the sargassum fusiforme polysaccharide is influenced due to the strong oxidation activity of the hydrogen peroxide.

Disclosure of Invention

The invention aims to solve the technical problem of providing a method for decolorizing sargassum fusiforme polysaccharide in the prior art, which has high decolorizing efficiency and good decolorizing effect and does not influence the biological activity of the sargassum fusiforme polysaccharide.

The technical scheme adopted by the invention for solving the technical problems is as follows: a decolorization method for sargassum fusiforme polysaccharide is characterized by comprising the following steps:

(1) activating alumina, namely uniformly mixing the activated alumina, magnesium oxide and kaolin according to a certain ratio, uniformly stirring distilled water, filling the mixture into a chromatographic column, and pressing the chromatographic column under a certain pressure to obtain an alumina-magnesia-kaolin composite chromatographic column;

(2) dissolving sargassum fusiforme polysaccharide to be decolorized in water to prepare sargassum fusiforme polysaccharide solution with a certain concentration, and centrifuging to obtain supernatant;

(3) and (3) passing the supernatant obtained in the step (2) through the alumina-magnesia-kaolin composite chromatographic column, eluting, and carrying out vacuum freeze drying to obtain the decolorized sargassum fusiforme polysaccharide.

Further, in the step (1), the mixing mass part ratio of the aluminum oxide, the magnesium oxide and the kaolin is as follows: 1-2: 1-4: 1-2. Thereby enabling the mixture to better exert the adsorption effect, enabling the alumina to better exert the effect of the filter aid, better solving the problem of slow flow rate of chromatography, and enabling the liquid after adsorption to be clearer and more transparent.

Further, the pressure of the compression column in the step (1) is 10-100 bar. Thereby realizing stable column pressing without pressure shaking.

Further, the concentration of the sargassum fusiforme polysaccharide solution in the step (2) is 1/15-1/10.

Further, the activation process of the alumina in the step (1) is as follows: and soaking the alumina in distilled water, naturally settling until the supernatant is not turbid, and drying the precipitate at 200-250 ℃ for 4-12 h.

Furthermore, the material of the chromatographic column in the step (1) is pressure-resistant glass or metal which can resist 100 bar-150 bar.

Further, the centrifugal speed in the step (2) is 6000 rpm-10000 rpm, and the centrifugal time is 20 min-40 min, so that the centrifugal effect is ensured.

Further, the elution conditions in the step (3) are as follows: the eluent is distilled water, the elution pressure is 100bar, the elution speed is 2 BV/h-3 BV/h, and the elution volume is 3 BV-5 BV. The elution is carried out by adopting higher pressure, and the composite filler is combined, so that the problem that the flow rate of a single magnesium oxide column is low and even the column is blocked is avoided, and the foundation is laid for realizing industrial production.

Further, preferably, the alumina is neutral alumina.

Further, preferably, the magnesium oxide is light magnesium oxide.

Compared with the prior art, the invention has the advantages that:

(1) the invention adopts a physical adsorption method for decolorization, and compared with the traditional method adopting chemical decolorization, the invention has smaller influence on the biological activity of the sargassum fusiforme polysaccharide in the decolorization process.

(2) According to the invention, alumina, magnesium oxide and kaolin are used as chromatographic fillers for adsorption, and compared with common activated carbon which is physically adsorbed, the adsorption effect is better, and the liquid after adsorption is clearer and more transparent.

(3) According to the invention, the alumina, the magnesium oxide and the kaolin are mixed and then used as chromatographic fillers for adsorption, and compared with the single use of alumina, the adsorption capacity is larger and the adsorption effect is better.

(4) According to the invention, alumina, magnesia and kaolin are mixed and then used as chromatography fillers for adsorption, compared with the single use of magnesia, the magnesia has a fine particle size, so that a chromatography column is seriously blocked in the chromatography process, the flow rate is extremely slow, and the phenomenon that a sample loading liquid cannot be pumped can occur in the single magnesia along with the test; the method mixes activated alumina and magnesium oxide and then uses the mixture as chromatographic packing, and the alumina has adsorption capacity and also serves as a filter aid, so that the problem of slow chromatographic flow rate is solved; the addition of the kaolin can adsorb pigments and small particles in a wider spectrum, so that the adsorbed liquid is clearer and more transparent.

(5) The invention can realize on-line elution, further realize continuous production and meet the requirement of scale.

Detailed Description

The present invention will be described in further detail with reference to examples.