阅读说明：本技术 一种杜仲总黄酮的提取方法及其在抗氧化剂和降糖剂中的应用 (Method for extracting total flavonoids of eucommia ulmoides and application of total flavonoids of eucommia ulmoides in antioxidant and hypoglycemic agent ) 是由 欧阳辉 于 2019-12-06 设计创作，主要内容包括：本发明涉及杜仲提取物的研究,具体涉及一种杜仲总黄酮的提取方法及其在抗氧化剂和降糖剂中的应用,本发明以杜仲为原料,微波辅助法提取杜仲中的总黄酮,总黄酮的最佳提取条件通过设置单因素实验和响应面优化来确定,并提供了总黄酮的抗氧化性及降糖活性的应用。(The invention relates to research of eucommia ulmoides extracts, in particular to an extraction method of total flavonoids of eucommia ulmoides and application of the total flavonoids of eucommia ulmoides in an antioxidant and a hypoglycemic agent.)

1. A method for extracting total flavonoids of eucommia ulmoides is characterized by comprising the following steps:

(1) drying and crushing eucommia ulmoides, and then sieving the eucommia ulmoides by a 60-80-mesh sieve;

(2) mixing the eucommia ulmoides powder obtained in the step (1) with ethanol, and mixing the mixture in a material-liquid ratio of 1: 20-60 percent of ethanol with the concentration of 60-80 percent;

(3) microwave extracting for 15-35min, and cooling for separation.

2. The method for extracting total flavonoids from eucommia ulmoides as claimed in claim 1, wherein the eucommia ulmoides is extracted from eucommia ulmoides samara.

3. The method for extracting total flavonoids from eucommia ulmoides as claimed in claim 1, wherein the concentration of ethanol is 70%.

4. Use of the extract obtained by the extraction process according to claims 1-3, characterized in that the extract obtained is used as an antioxidant.

5. Use of the extract obtained by the extraction process according to claims 1-3, characterized in that the obtained extract is used as a hypoglycemic agent.

6. The use of claim 5, wherein the extract is formulated with pharmaceutically acceptable excipients into an oral dosage form.

Technical Field

The invention belongs to research on eucommia ulmoides extracts, and particularly relates to an extraction method of total flavonoids of eucommia ulmoides and application of the total flavonoids of eucommia ulmoides in an antioxidant and a hypoglycemic agent.

Background

The fruit of eucommia ulmoides is developed from 2 oblong carpels, which are essentially nuts surrounded by thin, leathery wings and are also commonly referred to as "winged fruits". The eucommia ulmoides samara is flat and oblong. The periphery of the fruit is provided with wings, winged leathers and slightly concave tops, and the growing period of the fruit is 4-10 months. Joints are arranged at the joints of the ovary handle phase and the fruit stalk phase. The seeds are flat and linear, the length is 1.4-2.5cm, the width is 5mm, the thickness is about 2mm, the two ends are round, and the two ends are slightly concave.

Eucommia bark is a rare and old traditional Chinese medicinal material, and various scholars determine active substances of the eucommia bark in order to prove the disease treatment mechanism of the eucommia bark, and carry out a great deal of research on chemical components of the eucommia bark, wherein the main components comprise organic compounds such as lignans and flavonoids, and mineral elements such as calcium, iron, manganese, zinc, selenium and the like.

The "extraction process optimization of total flavonoids from eucommia ulmoides" in No. 20 and No. 12 of 2011 "of pharmaceutical technology" discloses a method for extracting total flavonoids from eucommia ulmoides by using a microwave extraction method, which comprises the following steps: weighing 10g of eucommia ulmoides powder (sieving with a 60-mesh sieve), respectively extracting by a reflux extraction method (adding 200mL of 60% ethanol, refluxing in water bath for 60min, filtering, extracting for 2 times, combining filtrates, fixing the volume to 100mL), an ultrasonic extraction method (placing in a 500mL conical flask, adding 200mL of 60% ethanol, performing ultrasonic extraction at room temperature for 60min, filtering, fixing the volume of the filtrate to 100mL), a microwave extraction method (adding 200mL of 60% ethanol, heating and extracting for 120s under the condition of microwave power of 200W, filtering, extracting for 2 times, combining the filtrates, fixing the volume to 100mL), and the like. However, the extraction rate of this method is not high, and application of the extract is not studied.

Disclosure of Invention

In order to solve the problems in the prior art, the invention provides a novel method for extracting total flavonoids from eucommia ulmoides and application of the total flavonoids in antioxidants and hypoglycemic agents through research.

The scheme of the invention is that the method for extracting the total flavonoids of eucommia ulmoides comprises the following steps:

drying and crushing eucommia ulmoides, and then sieving the eucommia ulmoides by a sieve of 60-80 meshes;

(2) mixing the eucommia ulmoides powder obtained in the step (1) with ethanol, and mixing the mixture in a material-liquid ratio of 1: 20-60 percent of ethanol with the concentration of 60-80 percent;

(3) microwave extracting for 15-35min, and cooling for separation.

In another preferred scheme of the invention, the eucommia ulmoides is extracted from eucommia ulmoides samara.

In another preferred embodiment of the present invention, the ethanol concentration is 70%.

The invention also provides application of the extract obtained by the extraction method, wherein the application of the extract is to use the extract for an antioxidant.

The invention also provides another application of the extract obtained by the extraction method, and the application of the extract is to use the extract in a hypoglycemic agent. More preferably, the obtained extract is mixed with pharmaceutically acceptable adjuvants to make into oral preparation.

The antioxidants are mostly used in food preservation medicine and the like, and the research finds that the artificially synthesized antioxidants such as BHT, TBHQ and the like have certain toxicity although having strong antioxidant capacity, so that the search for safe and natural food antioxidants is increasingly a research hotspot. The antioxidant disclosed by the invention has the advantages of safety and no toxicity, and can be widely applied to food additives and used as the antioxidant.

α -glucosidase inhibitor is often used for treating type II diabetes mellitus, can effectively reduce postprandial blood sugar level and reduce the occurrence of diabetic complications, so that finding an efficient α -glucosidase inhibitor from natural products becomes a research hotspot in recent years, and natural products such as citrus peel, liquorice, mulberry leaf, garden burnet, grapefruit, dalbergia wood, Pu' er tea, balsam pear, ginkgo and the like are reported to be rich in components such as flavone, polyphenol, polysaccharide and the like, so the research on an optimization process for extracting the flavone from the eucommia samara has high practical significance and wide application prospect for improving the economic value of eucommia.

The antioxidant activity of the eucommia samara extract is researched by observing the DPPH free radical eliminating capacity and the ABTS + caused absorbance change eliminating capacity through DPPH and ABTS methods, and the in-vitro hypoglycemic activity of the eucommia samara total flavonoids is researched through the inhibition rate of α -glucosidase, so that important data is provided for the evaluation, development and utilization of the health care function of the eucommia samara.

Drawings

FIG. 1 is a graph showing the effect of microwave time on the extraction rate of total flavonoids according to the present invention;

FIG. 2 is a graph showing the effect of feed liquid ratio on total flavone extraction rate in the example of the present invention;

FIG. 3 is a graph showing the effect of ethanol concentration on total flavone extraction rate according to the present invention;

FIG. 4 is a graph showing the effect of microwave temperature on total flavone extraction rate according to the present invention;

FIG. 5a is a graph of the interaction curve of microwave time versus feed-to-liquid ratio for an embodiment of the present invention;

FIG. 5b is a graph of the interaction curve of microwave time with ethanol concentration for an embodiment of the present invention;

FIG. 5c is a graph of the interaction curve of temperature and microwave time for an embodiment of the present invention;

FIG. 5d is a graph of the interaction curve of ethanol concentration versus feed-to-liquid ratio for the examples of the present invention;

FIG. 5e is a graph of the interaction surface of temperature versus feed-to-liquid ratio for an embodiment of the present invention;

FIG. 5a is a graph of the interaction of temperature with ethanol concentration for an embodiment of the present invention;

FIG. 6 is a graph of the clearance of different Vc solution concentrations versus DPPH;

FIG. 7 is a graph of the clearance of DPPH versus concentration of different total flavonoids;

FIG. 8 is a graph of the scavenging capacity of Trolox standard solutions for ABTS free radicals;

FIG. 9 is a graph of total flavonoid solution scavenging ability of ABTS free radicals;

FIG. 10 is a graph of the inhibition of α -glucosidase at different acarbose solution concentrations;

FIG. 11 is a graph of the inhibition rate of α -glucosidase by different total flavone solution concentrations.

Detailed Description

The present invention will now be described in detail with reference to the drawings, which are given by way of illustration and explanation only and should not be construed to limit the scope of the present invention in any way. Furthermore, features from embodiments in this document and from different embodiments may be combined accordingly by a person skilled in the art from the description in this document.