阅读说明：本技术 一种从水产品加工副产物中高效富集n-3PUFA脂质的方法 (Method for efficiently enriching n-3PUFA lipid from aquatic product processing by-products ) 是由 王�琦 岳大鹏 张维农 胡志雄 赵秀举 张燕鹏 贺军波 于 2019-11-13 设计创作，主要内容包括：本发明公开了一种从水产品加工副产物中高效富集n-3PUFA脂质的方法,包括以下步骤:S1、将水产品加工副产物洗净后破碎,得到破碎后的副产物；S2、将破碎后的副产品和无水乙醇按照1:6-10g/mL的料液比混合均匀,得到混合物,然后在混合物中加入超纯水,直至混合物中乙醇的体积占比为75-85％,得到待处理物；S3、将待处理物高速破碎,得到浆液,备用；S4、将浆液进行过滤,得到乙醇提取液；S5、去除乙醇提取液中的乙醇,得到二次提取液,备用；S6、将二次提取液和丙酮按照1:5-10g/mL的质量体积比混合均匀,然后离心后,去除丙酮上清液,得到固体提取物,并脱解固体提取物中残留的丙酮后,即得到n-3PUFA脂质。本发明提供一种从水产品加工副产物中高效富集n-3PUFA脂质的方法。(The invention discloses a method for efficiently enriching n-3PUFA lipid from aquatic product processing byproducts, which comprises the following steps of S1, washing and crushing the aquatic product processing byproducts to obtain crushed byproducts; s2, uniformly mixing the crushed by-product and absolute ethyl alcohol according to the feed-liquid ratio of 1:6-10g/mL to obtain a mixture, and then adding ultrapure water into the mixture until the volume ratio of the ethyl alcohol in the mixture is 75-85% to obtain a substance to be treated; s3, crushing the object to be processed at high speed to obtain slurry for later use; s4, filtering the serous fluid to obtain an ethanol extracting solution; s5, removing ethanol in the ethanol extract to obtain a secondary extract for later use; s6, uniformly mixing the secondary extracting solution and acetone according to the mass-volume ratio of 1:5-10g/mL, centrifuging, removing acetone supernatant to obtain a solid extract, and removing residual acetone in the solid extract to obtain the n-3PUFA lipid. The invention provides a method for efficiently enriching n-3PUFA lipid from aquatic product processing byproducts.)

1. A method for efficiently enriching n-3PUFA lipid from aquatic product processing byproducts is characterized by comprising the following steps:

s1, washing and crushing the aquatic product processing by-product to obtain a crushed by-product for later use;

s2, uniformly mixing the crushed by-product and absolute ethyl alcohol according to a material-liquid ratio of 1:6-10g/mL to obtain a mixture, and then adding ultrapure water into the mixture until the volume ratio of the ethyl alcohol in the mixture is 75-85% to obtain a substance to be treated for later use;

s3, crushing the object to be processed at high speed to obtain slurry for later use;

s4, filtering the serous fluid to obtain an ethanol extracting solution for later use;

s5, removing ethanol in the ethanol extracting solution to obtain a secondary extracting solution for later use;

s6, uniformly mixing the secondary extracting solution and acetone according to the mass-volume ratio of 1:5-10g/mL, then centrifuging, removing acetone supernatant obtained after centrifuging, reserving solid extract, and removing residual acetone in the solid extract to obtain the n-3PUFA lipid.

2. The method for efficiently enriching n-3PUFA lipids from by-products of aquatic product processing according to claim 1, wherein when the crushed by-products and absolute ethanol are uniformly mixed in a feed-to-liquid ratio of 1:6-10g/mL in S2, the volume X of the absolute ethanol to be added is calculated as follows:

X＝ML(F+W)

wherein M is the mass of the crushed by-product; l is the feed-liquid ratio of the crushed by-product to absolute ethyl alcohol; w is the water content of the crushed by-product; f is the volume ratio of the ethanol in the substance to be treated.

3. The method for efficiently enriching n-3PUFA lipids according to claim 2, wherein the volume x of the ultrapure water added to the mixture in S2 is calculated as follows:

x＝M*F-X。

4. the method for efficiently enriching n-3PUFA lipids from the by-products of aquatic product processing according to any one of claims 1 to 3, wherein the by-products of aquatic product processing are crayfish heads.

5. The method for efficiently enriching n-3PUFA lipids in the by-products of aquatic product processing according to claim 4, wherein the crayfish head is chopped into 0.5-1cm square pieces in S1.

6. The method for efficiently enriching n-3PUFA lipids from byproducts of aquatic product processing according to claim 4, wherein the acetone remaining in the solid extract is removed, and the n-3PUFA lipids are obtained by washing the solid extract with absolute ethanol and then removing the absolute ethanol remaining in the solid extract.

Technical Field

The present invention relates to the field of n-3PUFA lipid extraction. More particularly, the invention relates to a method for efficiently enriching n-3PUFA lipid from byproducts generated in aquatic product processing.

Background

The n-3 polyunsaturated fatty acid, i.e. n-3PUFA lipid, is an important nutrient substance, has various physiological functions, can be used as a regulator of gene expression to directly and independently regulate the gene expression, and widely influences related metabolic and physiological pathological phenomena in animals.

China has numerous lakes and rivers and abundant aquatic resources. But the utilization rate of the processing by-products of the existing aquatic products is not high, and the problems of more primary processing mode, less fine and deep processing and poor market demand adaptability still exist. The finished product mainly focuses on gelatin, flavored food and flavor seasoning, the fish oil product is relatively few, the n-3PUFA lipid of the fish oil is rich, and especially eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) play a significant role in improving memory and sleep and reducing the risk of cardiovascular and cerebrovascular diseases, and the market demand is large. The fish head is an important byproduct in the fish processing process, the fish head contains rich grease, the content of n-3PUFA in the grease is generally 20-30%, and a method for efficiently enriching n-3PUFA lipid in the aquatic product processing byproduct is urgently needed.

Disclosure of Invention

The invention provides a method for efficiently enriching n-3PUFA lipid from aquatic product processing byproducts.

To achieve these objects and other advantages in accordance with the present invention, there is provided a method for efficiently enriching n-3PUFA lipids from by-products of aquatic product processing, comprising the steps of:

s1, washing and crushing the aquatic product processing by-product to obtain a crushed by-product for later use;

s2, uniformly mixing the crushed by-product and absolute ethyl alcohol according to a material-liquid ratio of 1:6-10g/mL to obtain a mixture, and then adding ultrapure water into the mixture until the volume ratio of the ethyl alcohol in the mixture is 75-85% to obtain a substance to be treated for later use;

s3, crushing the object to be processed at high speed to obtain slurry for later use;

s4, filtering the serous fluid to obtain an ethanol extracting solution for later use;

s5, removing ethanol in the ethanol extracting solution to obtain a secondary extracting solution for later use;

s6, uniformly mixing the secondary extracting solution and acetone according to the mass-volume ratio of 1:5-10g/mL, then centrifuging, removing acetone supernatant obtained after centrifuging, reserving solid extract, and removing residual acetone in the solid extract to obtain the n-3PUFA lipid.

Preferably, in the method for efficiently enriching n-3PUFA lipids from by-products of aquatic product processing, when the crushed by-products and absolute ethanol are uniformly mixed in a feed-to-liquid ratio of 1:6-10g/mL in S2, the method for calculating the volume X of the absolute ethanol that is not required to be added is as follows:

X＝ML(F+W)

wherein M is the mass of the crushed by-product; l is the feed-liquid ratio of the crushed by-product to absolute ethyl alcohol; w is the water content of the crushed by-product; f is the volume ratio of the ethanol in the substance to be treated.

Preferably, in the method for efficiently enriching n-3PUFA lipids from by-products of aquatic product processing, the volume x of ultrapure water added to the mixture in S2 is calculated as follows:

x＝M*F-X。

preferably, in the method for efficiently enriching the n-3PUFA lipid from the aquatic product processing by-products, the crayfish heads are used as the aquatic product processing by-products.

Preferably, in the method for efficiently enriching n-3PUFA lipid from the by-products of aquatic product processing, the heads of the crayfish are chopped into 0.5-1cm square pieces in S1.

Preferably, in the method for efficiently enriching the n-3PUFA lipids from the byproducts of the aquatic product processing, when removing the residual acetone in the solid extract, the solid extract is washed by using absolute ethyl alcohol, and then the residual absolute ethyl alcohol in the solid extract is removed, so that the n-3PUFA lipids are obtained.

The invention improves the purity of the n-3PUFA lipid to a great extent from the aspects of actual safety and high efficiency, increases the extraction purity of the n-3PUFA lipid from 20-30 percent to 50-60 percent, and realizes the high-efficiency enrichment of the n-3PUFA lipid in the by-products of aquatic product processing.

Additional advantages, objects, and features of the invention will be set forth in part in the description which follows and in part will become apparent to those having ordinary skill in the art upon examination of the following or may be learned from practice of the invention.

Detailed Description

The present invention is further described in detail below with reference to examples so that those skilled in the art can practice the invention with reference to the description.

It is to be noted that the experimental methods described in the following embodiments are all conventional methods unless otherwise specified, and the reagents and materials are commercially available unless otherwise specified.

< example 1>

A method for efficiently enriching n-3PUFA lipid from aquatic product processing byproducts comprises the following steps:

s1, cleaning and crushing the crayfish heads to obtain 0.5cm square fragments, and obtaining crushed byproducts for later use;

s2, uniformly mixing the crushed by-product and absolute ethyl alcohol according to a material-liquid ratio of 1:6g/mL to obtain a mixture, and then adding ultrapure water into the mixture until the volume of the ethyl alcohol in the mixture accounts for 75% to obtain a substance to be treated for later use;

s3, crushing the object to be processed at high speed to obtain slurry for later use;

s4, filtering the serous fluid to obtain an ethanol extracting solution for later use;

s5, removing ethanol in the ethanol extracting solution to obtain a secondary extracting solution for later use;

s6, uniformly mixing the secondary extracting solution and acetone according to the mass-volume ratio of 1:5g/mL, then centrifuging, removing acetone supernatant obtained after centrifuging, reserving solid extract, and removing residual acetone in the solid extract to obtain the n-3PUFA lipid.

Wherein when removing the residual acetone in the solid extract, the solid extract is washed by absolute ethyl alcohol, and then the residual absolute ethyl alcohol in the solid extract is removed, so as to obtain the n-3PUFA lipid.

< example 2>

A method for efficiently enriching n-3PUFA lipid from aquatic product processing byproducts comprises the following steps:

s1, cleaning and crushing the crayfish heads to obtain 0.8cm square fragments, and obtaining crushed byproducts for later use;

s2, uniformly mixing the crushed by-product and absolute ethyl alcohol according to a material-liquid ratio of 1:8g/mL to obtain a mixture, and then adding ultrapure water into the mixture until the volume of the ethyl alcohol in the mixture accounts for 80% to obtain a substance to be treated for later use;

s3, crushing the object to be processed at high speed to obtain slurry for later use;

s4, filtering the serous fluid to obtain an ethanol extracting solution for later use;

s5, removing ethanol in the ethanol extracting solution to obtain a secondary extracting solution for later use;

s6, uniformly mixing the secondary extracting solution and acetone according to the mass-volume ratio of 1:7g/mL, then centrifuging, removing acetone supernatant obtained after centrifuging, reserving solid extract, and removing residual acetone in the solid extract to obtain the n-3PUFA lipid.

Wherein when removing the residual acetone in the solid extract, the solid extract is washed by absolute ethyl alcohol, and then the residual absolute ethyl alcohol in the solid extract is removed, so as to obtain the n-3PUFA lipid.

< example 3>

A method for efficiently enriching n-3PUFA lipid from aquatic product processing byproducts comprises the following steps:

s1, cleaning and crushing the crayfish heads to obtain 1cm square fragments, and obtaining crushed byproducts for later use;

s2, uniformly mixing the crushed by-product and absolute ethyl alcohol according to a material-liquid ratio of 1:10g/mL to obtain a mixture, and then adding ultrapure water into the mixture until the volume of the ethyl alcohol in the mixture accounts for 85% to obtain a substance to be treated for later use;

s3, crushing the object to be processed at high speed to obtain slurry for later use;

s4, filtering the serous fluid to obtain an ethanol extracting solution for later use;

s5, removing ethanol in the ethanol extracting solution to obtain a secondary extracting solution for later use;

s6, uniformly mixing the secondary extracting solution and acetone according to the mass-volume ratio of 1:10g/mL, then centrifuging, removing acetone supernatant obtained after centrifuging, reserving solid extract, and removing residual acetone in the solid extract to obtain the n-3PUFA lipid.

Wherein when removing the residual acetone in the solid extract, the solid extract is washed by absolute ethyl alcohol, and then the residual absolute ethyl alcohol in the solid extract is removed, so as to obtain the n-3PUFA lipid.

Embodiments 1 to 3 of the present invention efficiently enrich n-3PUFA lipids in aquatic product processing by-products, so that the extraction purity of n-3PUFA lipids is increased from 20 to 30% to 50 to 60%, thereby achieving efficient enrichment of n-3PUFA lipids in aquatic product processing by-products.

While embodiments of the invention have been described above, it is not limited to the applications set forth in the description and the embodiments, which are fully applicable to various fields of endeavor for which the invention may be embodied with additional modifications as would be readily apparent to those skilled in the art, and the invention is therefore not limited to the details given herein and to the embodiments shown and described without departing from the generic concept as defined by the claims and their equivalents.