阅读说明：本技术 一种含***二酚纳米脂质体的润肤水及制备方法 (Skin lotion containing cannabidiol nanoliposome and preparation method thereof ) 是由 刘胜贵 王钲霖 薛红芬 付彬彬 李智高 孔令羽 于 2019-12-06 设计创作，主要内容包括：本发明公开了一种含大麻二酚纳米脂质体的润肤水及制备方法,其特征在于,所述润肤水由如下质量百分比的原料组成：0.1-1%大麻二酚纳米脂质体、5-10%甘油、0.5-3%透明质酸、0.1-1%神经酰胺、1-5%海藻糖、0.001-0.01%苯氧乙醇、去离子水。大麻二酚(CBD)有诸多生理功效：如抗菌消炎、祛痘舒缓、抗氧化等功效,但由于CBD不溶于水限制了其应用领域和生物利用度。本发明将脂溶性的CBD制成纳米级的大麻二酚脂质体,不仅提高了CBD水溶性和稳定性,同时促进CBD的透皮吸收效果,使其快速有效的穿透皮肤表层,直达肌肤深层并缓慢释放CBD,使其功效更持久。本发明所述润肤水,外观清澈透明,无沉淀或CBD结晶析出,不含酒精、激素、色素、香精等成分,配方柔和安全,具有高效保湿、抗菌消炎、祛痘舒缓、抗氧化等多重功效。(The invention discloses a skin lotion containing cannabidiol nanoliposomes and a preparation method thereof, and is characterized in that the skin lotion is prepared from the following raw materials in percentage by mass: 0.1-1% cannabidiol nanoliposome, 5-10% glycerin, 0.5-3% hyaluronic acid, 0.1-1% ceramide, 1-5% trehalose, 0.001-0.01% phenoxyethanol and deionized water. Cannabidiol (CBD) has a number of physiological effects: such as antibacterial and anti-inflammatory effects, acne removal and soothing effects, oxidation resistance and the like, but the application field and the bioavailability of the CBD are limited due to the insolubility of the CBD in water. According to the invention, the fat-soluble CBD is prepared into the nanoscale cannabidiol liposome, so that the water solubility and stability of the CBD are improved, the transdermal absorption effect of the CBD is promoted, the CBD can quickly and effectively penetrate through the surface layer of the skin and directly reach the deep layer of the skin, the CBD is slowly released, and the effect is more durable. The skin moistening water disclosed by the invention is clear and transparent in appearance, free of precipitation or CBD crystallization, soft and safe in formula, free of components such as alcohol, hormone, pigment and essence, and has multiple effects of high-efficiency moisturizing, antibiosis and antiphlogosis, acne removal and soothing, oxidation resistance and the like.)

1. The skin lotion containing cannabidiol nanoliposomes is characterized by comprising the following raw materials in percentage by mass: 0.1-1% cannabidiol nanoliposome, 5-10% glycerin, 0.5-3% hyaluronic acid, 0.1-1% ceramide, 1-5% trehalose, 0.001-0.01% phenoxyethanol and the balance of deionized water.

2. The skin lotion containing cannabidiol nanoliposomes as claimed in claim 1, and the preparation method thereof, wherein the preparation method of the skin lotion comprises the following steps:

s1, sequentially adding glycerol, hyaluronic acid, ceramide and trehalose into deionized water according to the formula proportion, heating to 70-80 ℃, stirring for dissolving, and uniformly mixing;

s2, cooling to 40-50 ℃, sequentially adding the cannabidiol nanoliposome and phenoxyethanol into the solution obtained in the step S1, uniformly stirring to obtain a mixed solution, standing for 2 hours, filtering, and collecting filtrate;

s3, filtering the obtained filtrate by adopting a 0.45-micron microporous filter membrane for sterilization to obtain the skin moisturizing lotion.

3. The skin lotion containing cannabidiol nanoliposomes as claimed in claim 1 or 2, and the preparation method thereof, wherein the preparation method of cannabidiol nanoliposomes comprises the following steps:

(1) preparing an organic phase: accurately weighing cannabidiol, phospholipid and cholesterol according to a certain proportion, adding into an organic solvent, heating and stirring to fully dissolve the cannabidiol, the phospholipid and the cholesterol to obtain an organic phase;

(2) film preparation: evaporating the organic phase obtained in the step (1) on a rotary evaporator to remove the solvent, so that the residue forms a phospholipid film on the inner wall of the container;

(3) hydration: adding a hydration medium phosphate buffer solution into the container obtained in the step (2), and continuously performing rotary evaporation to enable a phospholipid film to fall off to obtain a cannabidiol liposome suspension;

(4) homogenizing: injecting the suspension into a high-pressure homogenizer for homogenization, and filtering and extruding through an extruder after homogenization;

(5) and (3) drying: and (4) carrying out spray drying on the extrusion liquid to obtain the cannabidiol nanoliposome.

4. The method of claim 3, wherein the phospholipid comprises any one of egg yolk lecithin, hydrogenated egg yolk lecithin, soybean lecithin, hydrogenated soybean lecithin, sphingomyelin, phosphatidylethanolamine, dimyristoylphosphatidylcholine (i.e., DMPC), dimyristoylphosphatidylglycerol (i.e., DMPG), dipalmitoylphosphatidylcholine, distearoylphosphatidylcholine, dioleoylphosphatidylcholine, dilauroylphosphatidylcholine, and combinations thereof; the organic solvent comprises methanol, ethanol, dichloromethane, chloroform and their mixture.

5. The method for preparing cannabidiol nanoliposomes according to claim 3, wherein the ratio of phospholipid to cholesterol is 2-6: 1, cannabidiol to phospholipid ratio of 1: 10 to 30.

6. The method for preparing cannabidiol nanoliposomes according to claim 3, wherein the rotary evaporation temperature is 40-60 ℃, the vacuum degree is 0.1-0.6 MPa, and the rotary evaporation time is 1-3 h.

7. The method for preparing cannabidiol nanoliposomes according to claim 3, wherein the phosphate buffer solution has a pH of 5-8 and a concentration of 0.01-0.1 mol/L.

8. The method for preparing cannabidiol nanoliposomes according to claim 3, wherein the homogenization is performed for 2-3 times, the homogenization pressure is 20-50 MPa, and the pore diameter of the filter membrane is less than 200 nm; spray drying conditions: the air inlet temperature is 160-200 ℃, and the air outlet temperature is 70-110 ℃.

Technical Field

The invention belongs to the technical field of cosmetics, and particularly relates to a skin lotion containing cannabidiol nanoliposomes and a preparation method thereof.

Background

Emollient water is a liquid skin care cosmetic with low viscosity and very fluid like water, and its most original and basic function is to supplement and maintain the water needed by human skin. However, with the consumption requirements of different levels, people do not satisfy the effects of moisturizing skin lotion, such as merely replenishing water and moisturizing skin, and also need to have other skin care and nourishing effects, such as diminishing inflammation, removing acne, soothing, whitening, resisting wrinkles, repairing and the like, and meanwhile, the used components are safe and have no side effect.

Cannabis has been increasingly appreciated by the world due to its important pharmacological and physiological activities of the active ingredient cannabinoid, and more than 100 phyto-cannabinoids have been isolated to date. Cannabidiol (CBD) with high content of cannabinoids is a main active ingredient in cannabinoids, is a non-addictive active ingredient, and has no neurotoxicity. In recent years, scientists find that Cannabidiol (CBD) is a powerful antioxidant and has a series of physiological activity functions of blocking breast cancer metastasis, resisting epilepsy, resisting spasm, resisting rheumatoid arthritis, resisting anxiety, resisting insomnia and the like. Due to its strong antioxidant activity, antibacterial and anti-inflammatory effects, anti-acne and soothing effects, anti-aging effects and repairing effects on damaged cells, people pay more and more attention to the anti-acne and anti-acne cream. Therefore, the cannabidiol has wide application prospect in the fields of cosmetics, medicines and the like.

Because cannabidiol is a fat-soluble compound and is insoluble in water, the cannabidiol cannot be dissolved and dispersed when being directly added into cosmetics, and is difficult to be absorbed and utilized by skin. The skin moistening water is mostly deionized water, and even if the cannabidiol is dissolved by the solubilizer, the cannabidiol is easy to recrystallize and separate out after a large amount of deionized water is added.

With the development of nanotechnology, nanomaterials are increasingly applied to the cosmetic industry, and functional active ingredients are wrapped in capsules with the diameter of nanometer, and the nanocapsules are used as carriers and automatically and uniformly slowly released to act on skin tissues, so that the functional ingredients are maintained in effective concentration for a long time, and the effects of stabilizing the effective ingredients, reducing the stimulation of special additives to the skin and the like are achieved. Because the nano-capsule particles are tiny, are easy to disperse and suspend in water to form a colloidal solution, are clear and transparent in appearance, and have unique properties different from those of common microcapsules, the nano-capsule particles are widely applied to many fields, particularly the fields of medicines and cosmetics.

The water solubility of the lipid-soluble substance can be obviously improved after the lipid-soluble substance is prepared into the liposome by a certain process. According to the invention, the cannabidiol nano-active substance with the particle size reaching the nanometer level is prepared by utilizing a liposome technology, and is added into the emollient water, so that the problems of water solubility and stability of CBD can be solved, the CBD is stably wrapped in the nano-liposome, and the transdermal absorption efficiency and bioavailability of the active ingredient CBD in the emollient water can be greatly improved.

At present, the cannabidiol nanoliposome serving as an active ingredient is not reported to be added into skin moistening water to play the effect.

Disclosure of Invention

The invention aims to solve the problems of easy skin allergy, unsafety and the like caused by alcohol, pigment, hormone and the like in skin moistening water in the current market; on the other hand, the problem of water solubility of cannabidiol with obvious skin care effect is solved, and the skin lotion containing the cannabidiol nano liposome and the preparation method are provided, the prepared cannabidiol liposome is clear and transparent after being dissolved in water, and no precipitate or CBD crystal precipitation exists; the skin lotion has good moisturizing effect, and also has multiple skin care effects of resisting bacteria, diminishing inflammation, removing acne, relieving, resisting oxidation and the like. The invention has simple components, does not contain alcohol, hormone, pigment, essence and the like, and has soft and safe formula.

In order to achieve the purpose, the invention adopts the following technical scheme:

the skin lotion containing cannabidiol nanoliposomes is characterized by comprising the following raw materials in percentage by mass: 0.1-1% cannabidiol nanoliposome, 5-10% glycerin, 0.5-3% hyaluronic acid, 0.1-1% ceramide, 1-5% trehalose, 0.001-0.01% phenoxyethanol and the balance of deionized water.

The invention provides a preparation method of cannabidiol nanoliposomes, which comprises the following steps:

(1) preparing an organic phase: cannabidiol, phospholipid and cholesterol are weighed accurately according to a certain proportion and added into an organic solvent, and the mixture is heated and stirred to be fully dissolved to obtain an organic phase.

(2) Film preparation: evaporating the organic phase obtained in step (1) on a rotary evaporator to remove the solvent, so that the residue forms a phospholipid film on the inner wall of the container.

(3) Hydration: and (3) adding a hydration medium phosphate buffer solution into the container obtained in the step (2), and continuously performing rotary evaporation to enable the phospholipid film to fall off to obtain the cannabidiol liposome suspension.

(4) Homogenizing: and (3) injecting the suspension into a high-pressure homogenizer for homogenizing for 2-3 times, wherein the homogenizing pressure is 30-50 MPa, filtering through a 100-200 nm filter membrane after homogenizing, and extruding.

(5) And (3) drying: and (4) carrying out spray drying on the extrusion liquid to obtain the cannabidiol nanoliposome.

The invention also provides a preparation method of the skin lotion containing the cannabidiol nanoliposome, which comprises the following steps:

s1, sequentially adding glycerol, hyaluronic acid, ceramide and trehalose into deionized water according to the formula proportion, heating to 70-80 ℃, stirring for dissolving, and uniformly mixing.

S2, cooling to 40-50 ℃, sequentially adding the cannabidiol nanoliposome and phenoxyethanol into the solution obtained in the step S1, uniformly stirring to obtain a mixed solution, standing for 2 hours, filtering, and collecting filtrate.

S3, filtering the obtained filtrate by adopting a 0.45-micron microporous filter membrane for sterilization to obtain the skin moisturizing lotion.

The water replenishing and moisturizing ingredient used in the invention has the following effects:

(1) glycerol: colorless, odorless, sweet in taste, clear, viscous and liquid in appearance, strong in hygroscopicity, capable of being mixed and dissolved with water and alcohols in any proportion, low in price and stable, and is a common humectant.

(2) Hyaluronic acid: also called hyaluronic acid, which is a mucus in the dermis layer of human skin. Hyaluronic acid has a special water retention effect, is the best substance found in nature in the present day, is called as an ideal natural moisturizing factor, can absorb water and carry water molecules which are hundreds of times of the weight of the hyaluronic acid to skin, and is a good transdermal absorption promoter while retaining moisture.

(3) Trehalose: trehalose is an extract from deep sea algae, and not only can inject rich and pure moisture into skin, but also can play a role in purifying skin oil. The seaweed extract also has a deep-positioned oil control system, and can deeply penetrate pores to play a role in controlling oil secretion in addition to deeply supplementing water and moisturizing. Therefore, the composition is suitable for the skin of the oil or the pox muscle. Trehalose is a non-reducing saccharide composed of specific disaccharide molecules, is very stable in properties, and can form a specific protective film on the cell surface under severe conditions such as high temperature, high cold, and drying and dehydration, and therefore, trehalose is not only an excellent humectant, but also a stabilizer and a protectant for bioactive substances. The trehalose and the hyaluronic acid are applied in a combined way, complement and synergize, and have the functions of biological preservation and intelligent moisture preservation.

(4) Ceramide: is an originally existing component of skin, and is intercellular lipid which is combined with cholesterol and unsaturated fatty acid to form the skin. Ceramides have a strong ability to associate with water molecules, and maintain the moisture of the skin through a network structure formed in the stratum corneum. The components not only have moisturizing effect, but also can help the skin to lock moisture for a long time, and prevent the skin moisture from losing.

The invention has the advantages and beneficial effects that:

(1) according to the invention, the cannabidiol insoluble in water is embedded in the phospholipid bilayer by a liposome embedding technology to form the closed vesicle with hydrophilic outside and lipophilic inside, the particle size of the liposome prepared by the technology reaches the nanometer level, the water solubility and the stability of CBD can be effectively improved, the CBD is stably encapsulated in the nanoliposome, and the transdermal absorption efficiency and the bioavailability of the CBD can be greatly increased.

(2) The skin lotion is added with the CBD nano liposome, so that the CBD can quickly and effectively penetrate through the surface layer of the skin and directly reach the deep layer of the skin to slowly release the CBD, and the effect is more durable.

(3) The skin moistening water disclosed by the invention is simple in components, safe, free of side effects, soft in formula, clear and transparent in appearance, free of precipitation or CBD crystallization, and free of alcohol, pigment, hormone, essence and the like.

(4) The lotion containing the CBD nanoliposome has good moisturizing effect, and also has multiple skin care effects of resisting bacteria, diminishing inflammation, removing acne, relieving, resisting oxidation and the like.

Detailed Description

The present invention is further illustrated by, but is not limited to, the following examples.