阅读说明：本技术 俄色茶和/或俄色茶提取物在制备防治非酒精性肝病和/或非酒精性肝损伤的药品中的应用 (Application of Russian tea and/or Russian tea extract in preparation of medicines for preventing and treating non-alcoholic liver disease and/or non-alcoholic liver injury ) 是由 程桂广 樊志峰 曹建新 杨美莲 赵天瑞 蔡圣宝 于 2019-11-05 设计创作，主要内容包括：本发明提供了俄色茶和/或俄色茶提取物在制备防治非酒精性肝病和/或非酒精性肝损伤的药品中的应用。俄色茶和/或俄色茶提取物对防治非酒精性肝病具备显著的防治作用。通过应用发现,俄色茶提取物能够有效地抑制肝系数的增加,从而抑制非酒精性脂肪肝的产生；俄色茶能够有效的抑制长期高脂饮食所造成的脂肪在肝细胞中的积累。俄色茶和/或俄色茶提取物用于制备防治非酒精性肝病的药品具有以下优势：俄色茶在我国的分布广泛,价格低廉；俄色茶为可食用的天然植物材料,安全性高；俄色茶提取和制备的工艺流程方便简单。(The invention provides application of Russian tea and/or Russian tea extract in preparation of medicines for preventing and treating nonalcoholic liver diseases and/or nonalcoholic liver injury. The Russian tea and/or the Russian tea extract has a remarkable prevention and treatment effect on the non-alcoholic liver disease. Through application, the Russian tea extract can effectively inhibit the increase of the liver coefficient, so that the production of non-alcoholic fatty liver is inhibited; the Russian tea can effectively inhibit the accumulation of fat in liver cells caused by long-term high-fat diet. The Russian tea and/or the Russian tea extract used for preparing the medicine for preventing and treating the non-alcoholic liver disease have the following advantages: the Russian tea is widely distributed in China and is low in price; the Russian tea is an edible natural plant material, and has high safety; the process flow of the extraction and preparation of the Russian tea is convenient and simple.)

1. Application of Russian tea and/or Russian tea extract in preparing medicine for preventing and treating non-alcoholic liver disease is provided.

2. The use of claim 1, wherein the non-alcoholic liver disease comprises non-alcoholic fatty liver disease and/or non-alcoholic fatty liver complications.

3. Application of Russian tea and/or Russian tea extract in preparing medicine for preventing and treating non-alcoholic liver injury is provided.

4. Application of Russian tea and/or Russian tea extract in preparing food, health product or medicine for protecting liver and/or inhibiting fat particle deposition in liver is provided.

5. The use according to any one of claims 1 to 4, wherein the medicament further comprises a pharmaceutically acceptable excipient.

6. The use of any one of claims 1 to 4, wherein the medicament is in the form of a powder, granule, pill, tablet, capsule, paste or decoction.

7. The use of any one of claims 1 to 4, wherein the Russian tea extract comprises Russian tea alcohol extract and/or Russian tea water extract.

8. The use of claim 7, wherein the Russian tea alcohol extract is prepared by the following method: mixing Russian tea with an ethanol water solution, performing ultrasonic extraction to obtain a crude extract, centrifuging the crude extract, and collecting the supernatant to obtain Russian tea ethanol extract; the volume percentage of the ethanol in the ethanol water solution is 75-85%.

9. The application of claim 8, wherein the temperature of ultrasonic extraction is 20-30 ℃, the time is 25-35 min, and the ultrasonic power is 30-40 Kw.

10. The use of claim 7, wherein the aqueous Russian tea extract is prepared by the following method: mixing the Russian tea with water, boiling for 0.8-1.5 h at 95-105 ℃ to obtain a crude extract, centrifuging the crude extract, and collecting the supernatant to obtain the Russian tea water extract.

Technical Field

The invention relates to the technical field of nonalcoholic liver disease treatment, in particular to application of Russian tea and/or Russian tea extract in preparation of a medicine for preventing and treating nonalcoholic liver disease and/or nonalcoholic liver injury.

Background

Non-alcoholic fatty liver disease (NAFLD) refers to a clinical pathological syndrome characterized mainly by excessive fat deposition in liver cells due to alcohol and other definite liver damage factors, and acquired metabolic stress liver injury closely related to insulin resistance and genetic susceptibility. Including Simple Fatty Liver (SFL), non-alcoholic steatohepatitis (NASH) and its associated cirrhosis. With the prevalence trend of obesity and its associated metabolic syndrome globalization.

NAFLD can directly cause diseases such as compensatory liver cirrhosis, liver cancer and the like, can also affect the progress of other chronic liver diseases, and is involved in the onset of type 2 diabetes and atherosclerosis. Malignant tumors associated with metabolic syndrome, arteriosclerotic cardiovascular and cerebrovascular diseases, and liver cirrhosis are important factors affecting the quality of life and life expectancy of patients with NAFLD. However, there is currently no clinical therapeutic drug specifically targeted at NAFLD, making NAFLD a great challenge in the contemporary medical field.

Disclosure of Invention

The invention aims to provide application of Russian tea and/or Russian tea extracts in preparation of medicines for preventing and treating nonalcoholic liver diseases and/or nonalcoholic liver injuries.

In order to achieve the above object, the present invention provides the following technical solutions:

the invention provides application of Russian tea and/or Russian tea extract in preparation of medicines for preventing and treating nonalcoholic liver diseases.

Preferably, the non-alcoholic liver disease comprises non-alcoholic fatty liver disease and/or non-alcoholic fatty liver complications.

The invention also provides application of the Russian tea and/or the Russian tea extract in preparation of medicines for preventing and treating non-alcoholic liver injury.

The invention also provides application of the Russian tea and/or the Russian tea extract in preparing food, health-care products or medicines for protecting the liver and/or inhibiting fat particle deposition in the liver.

Preferably, the medicine also comprises pharmaceutically acceptable auxiliary materials.

Preferably, the dosage form of the medicine comprises powder, granules, pills, tablets, capsules, paste or decoction.

Preferably, the russian tea extract comprises a russian tea alcohol extract and/or a russian tea water extract.

Preferably, the russian tea alcohol extract is prepared by the following method: mixing Russian tea with an ethanol water solution, performing ultrasonic extraction to obtain a crude extract, centrifuging the crude extract, and collecting the supernatant to obtain Russian tea ethanol extract; the volume percentage of the ethanol in the ethanol water solution is 75-85%.

Preferably, the temperature of ultrasonic extraction is 20-30 ℃, the time is 25-35 min, and the ultrasonic power is 30-40 Kw.

Preferably, the russian tea water extract is prepared by the following method: mixing the Russian tea with water, boiling for 0.8-1.5 h at 95-105 ℃ to obtain a crude extract, centrifuging the crude extract, and collecting the supernatant to obtain the Russian tea water extract.

The invention has the beneficial effects that: the invention provides application of Russian tea and/or Russian tea extract in preparation of medicines for preventing and treating nonalcoholic liver diseases. The Russian tea and/or the Russian tea extract has a remarkable prevention and treatment effect on the non-alcoholic liver disease. Through application, the Russian tea extract can effectively inhibit the increase of the liver coefficient, so that the production of non-alcoholic fatty liver is inhibited; the Russian tea can effectively inhibit the accumulation of fat in liver cells caused by long-term high-fat diet. The Russian tea and/or the Russian tea extract used for preparing the medicine for preventing and treating the non-alcoholic liver disease have the following advantages: the Russian tea is widely distributed in China and is low in price; the Russian tea is an edible natural plant material, and has high safety; the process flow of the extraction and preparation of the Russian tea is convenient and simple.

Drawings

FIG. 1 is a pathological section of rat liver in example 2, in which FIG. 1-A is a pathological section of rat liver in control group; FIG. 1-B is a pathological section of rat liver in model group; FIG. 1-C is a pathological section of liver of rat in low concentration group of alcohol extract; FIG. 1-D is a pathological section of liver of rat in high concentration group of alcohol extract; FIG. 1-E is a pathological section of liver of rats in a group with low concentration of water extract; FIG. 1-F is a pathological section of liver of rat in high-concentration water extract group;

FIG. 2 is a section view of immunohistochemical FAS in example 2, wherein FIG. 2-A is a section view of immunohistochemical FAS of a control group; FIG. 2-B is a section view of a model set immunohistochemical FAS; FIG. 2-C is a sectional view of an immunohistochemical FAS of the alcohol extract low concentration group; FIG. 2-D is a sectional view of an ethanol extract high concentration immunohistochemical FAS; FIG. 2-E are sectional views of immunohistochemical FAS of the water extract low concentration group; FIG. 2-F is a slice of an aqueous extract immunohistochemical FAS in a high concentration group.

Detailed Description

The invention provides an application of Russian tea and/or Russian tea extract in preparing a medicine for preventing and treating nonalcoholic liver diseases; the non-alcoholic liver disease preferably comprises non-alcoholic fatty liver disease and/or non-alcoholic fatty liver complications.

The invention also provides application of the Russian tea and/or the Russian tea extract in preparation of medicines for preventing and treating non-alcoholic liver injury.

The invention also provides application of the Russian tea and/or the Russian tea extract in preparing food, health-care products or medicines for protecting the liver and/or inhibiting fat particle deposition in the liver.

In the present invention, the pharmaceutical product preferably further comprises pharmaceutically acceptable excipients.

In the invention, the dosage form of the medicine preferably comprises powder, granules, pills, tablets, capsules, paste or decoction.

In the invention, the Russian tea extract preferably comprises a Russian tea alcohol extract and/or a Russian tea water extract.

In the invention, the Russian tea alcohol extract is prepared by adopting the following method: mixing Russian tea with an ethanol water solution, performing ultrasonic extraction to obtain a crude extract, centrifuging the crude extract, and collecting the supernatant to obtain Russian tea ethanol extract; the volume percentage content of the ethanol in the ethanol water solution is 75-85%, and the preferred volume percentage content is 80%.

In the invention, the Russian tea is collected from Sichuan province, and a sample is stored in the research institute of food safety in Yunnan province; the volume ratio of the Russian tea to the ethanol water solution is preferably 1: 9-11, and more preferably 1: 10; the ultrasonic extraction temperature is preferably 20-30 ℃, and more preferably 25 ℃; the number of times of ultrasonic extraction is preferably 3, and the time of each ultrasonic extraction is preferably 25-35 min, and more preferably 30 min; the power of ultrasonic extraction is preferably 30-40 Kw, and more preferably 35 Kw; the rotation speed of the centrifugation is preferably 3000-5000 rpm, and more preferably 4000 rpm; the centrifugation time is preferably 5-15 min, and more preferably 10 mim; after the Russian tea alcohol extract is obtained, the Russian tea alcohol extract is preferably subjected to freeze drying. Under the optimized condition, the pure Russian tea extract has the highest content of polyphenol brass, the best effects of resisting oxidation and inhibiting apoptosis and the obvious liver protection effect.

In the invention, the Russian tea aqueous extract is preferably prepared by the following method: mixing Russian tea with water, boiling for 0.8-1.5 h at 95-105 ℃ to obtain a crude extract, centrifuging the crude extract, and collecting the supernatant to obtain a Russian tea water extract; the volume ratio of the Russian tea to the water is preferably 1: 14-16, and more preferably 1: 15; the cooking temperature is preferably 100 ℃; the cooking time is preferably 1-1.2 h; the rotation speed of the centrifugation is preferably 3000-5000 rpm, and more preferably 4000 rpm; the time of the centrifugation is preferably 10 min; after the Russian tea water extract is obtained, the method preferably further comprises the steps of carrying out suction filtration and freeze drying on the Russian tea water extract. Under the optimized condition, the content of polysaccharide in the Russian tea water extract is highest, and the reported effect is obvious.

The technical solutions provided by the present invention are described in detail below with reference to examples, but they should not be construed as limiting the scope of the present invention.