阅读说明：本技术 一种利用植物秸秆制备l-***糖的方法 (Method for preparing L-arabinose by using plant straws ) 是由 宋建民 王德海 宛荣生 张琴 王颂 黄祥君 于 2019-11-14 设计创作，主要内容包括：本发明公开了糖类制备相关技术领域的一种利用植物秸秆制备L-阿拉伯糖的方法,包括如下步骤:按重量份计,向反应釜中加入2000～3000份植物秸秆、6000～7000份的水和10～15份的质量百分浓度为0.5～0.8％的强酸,在0.15～0.2MPa条件下加热至110～120℃处理45～50min；加入碱溶液,调节pH至6.5～7.5；过滤得到滤渣；加40～50份氮源和1～3份微生物并在富氧条件下维持40～42℃发酵60～72h；过滤得到发酵液；减压蒸馏得到浓度为75～80wt％的浓缩液；冷却至50～55℃后,加入L-阿拉伯糖晶种；在100～120℃条件下烘干制得L-阿拉伯糖；本发明利用植物秸秆作为生产原料,成本低廉；技术路线简单,便于推广应用；有效提高了生产效率；制得产品纯度优于行业普遍水准。(The invention discloses a method for preparing L-arabinose by utilizing plant straws in the related technical field of saccharide preparation, which comprises the following steps of adding 2000-3000 parts by weight of plant straws, 6000-7000 parts by weight of water and 10-15 parts by weight of strong acid with the mass percentage concentration of 0.5-0.8% into a reaction kettle, and heating to 110-120 ℃ under the condition of 0.15-0.2 MPa for processing for 45-50 min; adding an alkali solution, and adjusting the pH value to 6.5-7.5; filtering to obtain filter residue; adding 40-50 parts of nitrogen source and 1-3 parts of microorganism, and fermenting for 60-72 hours under the condition of oxygen enrichment and maintaining the temperature at 40-42 ℃; filtering to obtain fermentation liquor; carrying out reduced pressure distillation to obtain a concentrated solution with the concentration of 75-80 wt%; cooling to 50-55 ℃, and adding L-arabinose seed crystals; drying at 100-120 ℃ to obtain L-arabinose; the invention uses plant straw as raw material, which has low cost; the technical route is simple and is convenient to popularize and apply; the production efficiency is effectively improved; the purity of the prepared product is superior to the common level of the industry.)

1. A method for preparing L-arabinose by utilizing plant straws is characterized by comprising the following steps: the method comprises the following steps:

s1) adding the following raw materials in parts by weight into a reaction kettle: 2000-3000 parts of plant straw crushed into small sections, 6000-7000 parts of water and 10-15 parts of strong acid with the mass percentage concentration of 0.5-0.8%, and heating to 110-120 ℃ under the condition of 0.15-0.2 MPa for 45-50 min;

s2) cooling the mixture obtained in the step S1, adding an alkali solution, and adjusting the pH value to 6.5-7.5;

s3) filtering the mixture obtained in S2 to obtain filter residue;

s4) adding the following raw materials in parts by weight into the filter residue obtained in the step S4: 40-50 parts of nitrogen source and 1-3 parts of microorganism, and fermenting for 60-72 hours under the oxygen-enriched condition at 40-42 ℃;

s5) filtering the mixture obtained in the S4 to obtain a fermentation liquid;

s6) carrying out reduced pressure distillation on the fermentation liquor obtained in the step S5 to obtain a concentrated solution with the concentration of 75-80 wt%;

s7) cooling the concentrated solution obtained in the step S6 to 50-55 ℃, and adding L-arabinose seed crystals into the concentrated solution according to 0.1-0.3% of the total weight of the concentrated solution;

s8) drying the crystal precipitated in the S7 at the temperature of 100-120 ℃ to obtain the L-arabinose.

2. The method for preparing L-arabinose by using plant straws as claimed in claim 1, which comprises the following steps: the strong acid in S1 is one or more of nitric acid, sulfuric acid, hydrochloric acid and phosphoric acid.

3. The method for preparing L-arabinose by using plant straws as claimed in claim 1, which comprises the following steps: the alkali in S2 is one or more of sodium hydroxide, potassium hydroxide and calcium hydroxide.

4. The method for preparing L-arabinose by using plant straws as claimed in claim 1, which comprises the following steps: the microorganism in S4 is one or more of yeast and Escherichia coli.

5. The method for preparing L-arabinose by using plant straws as claimed in claim 4, which comprises the following steps: the yeast is one or more of candida, baker's yeast, beer yeast and pichia pastoris.

6. The method for preparing L-arabinose by using plant straws as claimed in claim 1, which comprises the following steps: the nitrogen source in S4 is one or more of yeast extract, yeast powder, corn steep liquor dry powder, soybean cake powder, ammonium sulfate and urea.

7. The method for preparing L-arabinose by using plant straws as claimed in any one of claims 1 to 6, which is characterized in that: the plant straw comprises one or more of wheat straw, rice straw, corn straw, sorghum straw, cottonseed hull and bagasse.

Technical Field

The invention relates to the technical field related to saccharide preparation, in particular to a method for preparing L-arabinose by utilizing plant straws.

Background

L-arabinose, also known as arabinose, fructose; is an aldopentose. In nature, L-arabinose, although widely present in the peel and hull of fruits and roughages, rarely exists in the form of monosaccharides, is usually combined with other monosaccharides, and exists in the form of heteropolysaccharides in gums, hemicelluloses, pectic acids, bacterial polysaccharides and certain glycosides.

The L-arabinose can inhibit the activity of sucrase in human intestinal tracts, so that the L-arabinose has the effect of inhibiting the absorption of sucrose; in addition, L-arabinose can also inhibit body fat accumulation, so that it can be used for preventing and treating obesity, hypertension, hyperlipidemia and other diseases.

Disclosure of Invention

Aiming at the technical problems of high production cost, long time consumption in the crystallization process, low crystallization purity, great difficulty in industrial production and the like in the prior art, the invention provides a method for preparing L-arabinose by utilizing plant straws.

In order to achieve the purpose, the technical scheme of the invention is as follows:

a method for preparing L-arabinose by utilizing plant straws comprises the following steps:

s1) adding the following raw materials in parts by weight into a reaction kettle: 2000-3000 parts of plant straw crushed into 3-5 cm small sections, 6000-7000 parts of water and 10-15 parts of strong acid with the mass percentage concentration of 0.5-0.8%, and heating to 110-120 ℃ under the condition of 0.15-0.2 MPa for treatment for 45-50 min;

s2) cooling the mixed solution obtained in the step S1, adding an alkali solution into the mixed solution, and adjusting the pH value to 6.5-7.5;

s3) filtering the mixture obtained in the step S2, discarding the filtrate, and reserving the filter residue for subsequent operation;

s4) adding the following raw materials in parts by weight into the filter residue obtained in the step S4: 40-50 parts of nitrogen source and 1-3 parts of microorganism, and fermenting for 60-72 hours under the oxygen-enriched condition at 40-42 ℃;

s5) filtering the mixture obtained in the step S4, discarding filter residues, and reserving fermentation liquor for subsequent operation;

s6) carrying out reduced pressure distillation on the fermentation liquor obtained in the step S5 to obtain a concentrated solution with the concentration of 75-80 wt%;

s7) cooling the concentrated solution obtained in the step S6 to 50-55 ℃, and adding L-arabinose seed crystals into the concentrated solution according to 0.1-0.3% of the total weight of the concentrated solution;

s8) drying the crystal precipitated in the S7 at the temperature of 100-120 ℃ to obtain the L-arabinose.

Preferably, the strong acid described in S1 is one or more of nitric acid, sulfuric acid, hydrochloric acid and phosphoric acid.

Preferably, the base described in S2 is one or more of sodium hydroxide, potassium hydroxide and calcium hydroxide.

Preferably, the microorganism described in S4 is one or more of yeast and escherichia coli.

Preferably, the yeast is one or more of candida, baker's yeast, brewer's yeast and pichia pastoris.

Preferably, the nitrogen source in S4 is one or more of yeast extract, yeast powder, corn steep liquor dry powder, soybean cake powder, ammonium sulfate and urea.

On the basis of the various schemes, the plant straws comprise one or more of wheat straws, rice straws, corn straws, sorghum straws, cottonseed hulls and bagasse.

The invention has the following advantages:

1. the invention uses plant straw as raw material, which has low cost;

2. the invention utilizes plant straws for production, can recycle the plant straws, and avoids the atmospheric pollution caused by the nowhere stacking of the straws or the burning of a large amount of straws;

3. the invention has simple technical route and convenient popularization and application;

4. the crystallization time of the method only needs 8-12 hours, and only in the step, at least 16 hours are saved compared with the traditional production process, so that the production efficiency is effectively improved;

5. the purity of the L-arabinose prepared by the invention is as high as 99.8 percent, which is superior to the general level of the industry.

Detailed Description

The following further describes the embodiments of the present invention. It should be noted that the description of the embodiments is provided to help understanding of the present invention, but the present invention is not limited thereto. In addition, the technical features involved in the embodiments of the present invention described below may be combined with each other as long as they do not conflict with each other.

In addition, the raw materials used in the invention, such as wheat straw, rice straw, corn straw, sorghum straw, cottonseed hull, bagasse and the like, are all taken from common crops, and even can be waste; strong acids such as nitric acid, sulfuric acid, hydrochloric acid, and phosphoric acid in the present invention; alkali such as sodium hydroxide, potassium hydroxide and calcium hydroxide; escherichia coli, and yeasts such as Candida, Saccharomyces cerevisiae, and Pichia pastoris; the nitrogen sources such as yeast extract, yeast powder, corn steep liquor dry powder, soybean cake powder, ammonium sulfate, urea and the like are all sold in the market and have no special requirement. The technical personnel in the field can implement the method according to the scheme by themselves without creative labor by combining with own experience.