阅读说明：本技术 一种降低断奶仔猪腹泻的饲用酶制剂及其制备方法 (Feed enzyme preparation for reducing diarrhea of weaned piglets and preparation method thereof ) 是由 张扬 孟勇 张秀红 尤雅 黄甜 李雪梅 于 2019-10-11 设计创作，主要内容包括：本发明提供了一种降低断奶仔猪腹泻的饲用酶制剂,该饲用酶制剂包括复合酶、茶多酚和载体,所述复合酶包括2000U/g葡萄糖氧化酶、3000U/g淀粉酶、8000U/g酸性蛋白酶、2000U/g酸性甘露聚糖酶和10000U/g木聚糖酶,所述载体包括玉米淀粉、石粉、麦饭石或米糠中的一种或几种。其制备方法包括如下步骤,复合酶制剂的各组分按配比称取后,先将复合酶的各组分混匀后,再与茶多酚和载体一同加入双轴高效混合机内混合,混合时间为10min～15min,混合后对成品进行混匀度检测,确保变异系数≤5％。本发明具有提高断奶仔猪的生长性能,降低料肉比和腹泻率,且本发明的饲用酶制剂为绿色环保添加剂。(The invention provides a feed enzyme preparation for reducing diarrhea of weaned piglets, which comprises a complex enzyme, tea polyphenol and a carrier, wherein the complex enzyme comprises 2000U/g glucose oxidase, 3000U/g amylase, 8000U/g acid protease, 2000U/g acid mannanase and 10000U/g xylanase, and the carrier comprises one or more of corn starch, stone powder, medical stone or rice bran. The preparation method comprises the following steps of weighing the components of the complex enzyme preparation according to the proportion, firstly uniformly mixing the components of the complex enzyme, then adding the components, tea polyphenol and a carrier into a double-shaft efficient mixer for mixing for 10-15 min, and detecting the degree of mixing of the finished product after mixing to ensure that the coefficient of variation is less than or equal to 5%. The feed enzyme preparation disclosed by the invention has the advantages that the growth performance of weaned piglets is improved, the feed conversion ratio and the diarrhea rate are reduced, and the feed enzyme preparation disclosed by the invention is a green and environment-friendly additive.)

1. The feed enzyme preparation for reducing diarrhea of weaned piglets is characterized by comprising a complex enzyme, tea polyphenol and a carrier, wherein the complex enzyme comprises 2000U/g glucose oxidase, 3000U/g amylase, 8000U/g acid protease, 2000U/g acid mannanase and 10000U/g xylanase, and the carrier comprises one or more of corn starch, stone powder, medical stone or rice bran.

2. The feed enzyme preparation as claimed in claim 1, wherein the feed enzyme preparation comprises, by weight, 15-25% of glucose oxidase, 5-15% of amylase, 10-20% of acid protease, 10-15% of acid mannase, 10-15% of xylanase, 3-5% of tea polyphenol and the balance of a carrier.

3. The feed enzyme preparation as claimed in claim 1, wherein the complex enzyme further comprises 10000U/g phytase, and the phytase accounts for 3-5% of the total weight of the feed enzyme preparation.

4. The feed enzyme preparation according to claim 1, wherein the fermentation strain of glucose oxidase is Pichia pastoris.

5. A feed enzyme preparation according to any one of claims 1 to 4, characterised in that it is prepared by a method comprising the steps of:

after weighing the components of the complex enzyme preparation according to the proportion, firstly, uniformly mixing the components of the complex enzyme, then adding the components, tea polyphenol and a carrier into a double-shaft efficient mixer for mixing for 10-15 min, and detecting the degree of mixing of the mixed product to ensure that the coefficient of variation is less than or equal to 5%.

Technical Field

The invention belongs to the technical field of feed enzymes, and particularly relates to a feed enzyme preparation and a preparation method thereof.

Background

The weaning of piglets is an important link in pig production, and due to weaning stress, piglets often produce diarrhea, so that the growth performance of the weaned piglets is affected, and the piglets can die in severe cases. In production, high dose of zinc oxide is often added into feed to reduce diarrhea rate, but the long-term use of the feed causes side effects.

Aiming at the problems, the feed additive capable of reducing diarrhea of weaned piglets and increasing weight gain is researched and developed, and the feed additive has extremely important significance for promoting the healthy development of the livestock industry.

Disclosure of Invention

Aiming at the defects in the prior art, the invention aims to provide the feeding enzyme preparation for reducing the diarrhea of weaned piglets and the preparation method thereof.

In order to achieve the above purpose, the solution adopted by the invention is as follows:

the feed enzyme preparation for reducing diarrhea of weaned piglets comprises a complex enzyme, tea polyphenol and a carrier, wherein the complex enzyme comprises 2000U/g glucose oxidase, 3000U/g amylase, 8000U/g acid protease, 2000U/g acid mannanase and 10000U/g xylanase, and the carrier comprises one or more of corn starch, stone powder, medical stone or rice bran.

Preferably, the feed enzyme preparation comprises, by weight, 15-25% of glucose oxidase, 5-15% of amylase, 10-20% of acid protease, 10-15% of acid mannase, 10-15% of xylanase, 3-5% of tea polyphenol and the balance of a carrier.

Preferably, the complex enzyme also comprises 10000U/g phytase, and the phytase accounts for 3-5% of the total weight of the feed enzyme preparation.

Preferably, the fermentation strain of the glucose oxidase is pichia pastoris.

A feeding enzyme preparation is prepared by the following steps:

after weighing the components of the feed enzyme preparation according to the proportion, firstly, uniformly mixing the components of the complex enzyme, then adding the components, the tea polyphenol and the carrier into a double-shaft efficient mixer for mixing for 10-15 min, and detecting the degree of mixing of the finished product after mixing to ensure that the coefficient of variation is less than or equal to 5%.

The invention has the beneficial effects that:

the feed enzyme preparation has the functions of promoting digestion, resisting oxidation and enhancing immunity in the bodies of the piglets, so that the intestinal tracts of the piglets can be developed and perfected, the intestinal microflora is improved, the diarrhea rate is reduced, the digestion utilization rate of nutrients is improved, the growth and development of the piglets are promoted, and the whole growth period is safer. The feed enzyme preparation improves the growth performance of weaned piglets, reduces the feed conversion ratio and the diarrhea rate, and is a green and environment-friendly additive.

The utilization rate of the feed for piglets is hindered by a large amount of fibers in the daily feed, and cellulose in the daily feed can be decomposed by the xylanase and the acidic mannase, so that the cellulose is effectively utilized, the acidic mannase is resistant to gastric acid, and the utilization rate of the feed is improved; the acidic protease can eliminate anti-nutritional factors in the feed, thereby improving the absorption of the piglet on the feed nutrients and utilizing the nutrients in the feed to the maximum extent; the amylase can supplement the shortage of endogenous enzymes of piglets and degrade starch which is difficult to degrade in the feed; the phytase is used for degrading phytic acid, releasing phosphorus in phytic acid molecules, reducing the use of inorganic phosphorus and reducing the discharge of redundant phosphorus, thereby reducing the environmental pollution caused by phosphorus discharge; the glucose oxidase can regulate the balance of intestinal microorganisms, improve the digestive capacity of the intestinal tract, improve the oxidation resistance, improve biochemical indexes and improve the immunity, and the glucose oxidase can reduce the permeability of cell membranes and inhibit creatine kinase from entering blood from muscle cells, thereby reducing the risk of animals from suffering from diseases. The tea polyphenols have antibacterial, antiviral, intestinal microbial environment improving, and antioxidant effects, and can promote absorption of piglet and utilize nutritional ingredients in daily ration, thereby improving conversion rate and production performance of daily ration.

Detailed Description

In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below. The examples, in which specific conditions are not specified, were conducted under conventional conditions or conditions recommended by the manufacturer. The reagents or instruments used are not indicated by the manufacturer, and are all conventional products available commercially.

The following describes a feeding enzyme preparation for reducing diarrhea of weaned pigs provided by the embodiment of the invention.

A feed enzyme preparation for reducing diarrhea of weaned piglets comprises a complex enzyme, tea polyphenol and a carrier, wherein the complex enzyme comprises 15% of 2000U/g glucose oxidase, 10% of 3000U/g amylase, 15% of 8000U/g acid protease, 10% of 2000U/g acid mannanase and 15% of 10000U/g xylanase by weight ratio, the carrier is 32% of medical stone and the tea polyphenol is 3%.

The fermentation strain of the glucose oxidase is pichia pastoris.

The preparation method comprises the following steps:

after weighing the components of the feed enzyme preparation according to the proportion, uniformly mixing the components of the complex enzyme, adding the components, tea polyphenol and a carrier into a double-shaft efficient mixer for mixing for 10min, and detecting the degree of mixing of a finished product after mixing to ensure that the coefficient of variation is less than or equal to 5%.