阅读说明：本技术 一种生物质食品高效防腐剂及其制备方法 (Efficient preservative for biomass food and preparation method thereof ) 是由 李骏 于 2019-11-28 设计创作，主要内容包括：本发明公开了一种生物质食品高效防腐剂及其制备方法,包括,乳链菌肽、溶菌酶、天然生物提取物、脂肪酸酯、尿囊素和溶剂,其中,以质量份数计,乳链菌肽为8～16份,溶菌酶为8～16份,天然生物提取物为6～12份,脂肪酸酯为5～10份,尿囊素为3～6份,溶剂为40～70份。本发明的生物质高效防腐剂采用生物质原料,且效果明显,在使用时只需0.002％～0.01％的用量即可保证其防腐功效,本身无毒并且有效用量极少,故对人体健康和环境无影响,其最佳的pH使用范围是5.5～7.5,但超出此范围仍能具有较好的防腐性能,具有pH值响应性。(The invention discloses a biomass food efficient preservative and a preparation method thereof, wherein the preservative comprises 8-16 parts of nisin, 8-16 parts of lysozyme, 6-12 parts of natural biological extract, 5-10 parts of fatty acid ester, 3-6 parts of allantoin and 40-70 parts of solvent by mass. The biomass high-efficiency preservative adopts biomass raw materials, has obvious effect, can ensure the preservative effect only by using 0.002-0.01 percent of the amount when in use, has no toxicity and little effective amount, has no influence on human health and environment, has the optimal pH application range of 5.5-7.5, still has better preservative property and pH value responsiveness when exceeding the range.)

1. The high-efficiency preservative for the biomass food is characterized in that: comprises the steps of (a) preparing a mixture of a plurality of raw materials,

nisin, lysozyme, a natural biological extract, fatty acid ester, allantoin and a solvent, wherein the nisin, the lysozyme, the natural biological extract, the fatty acid ester, the allantoin and the solvent are mixed according to the mass part,

8-16 parts of nisin, 8-16 parts of lysozyme, 6-12 parts of a natural biological extract, 5-10 parts of fatty acid ester, 3-6 parts of allantoin and 40-70 parts of a solvent.

2. The biomass food efficient preservative according to claim 1, characterized in that: the natural biological extract is one or two or more of cactus extract and propolis extract.

3. The biomass food efficient preservative according to claim 1, characterized in that: the preparation method of the natural biological extract comprises the following steps,

drying natural organisms in an oven at 25-35 ℃ for 3-5 hours in advance, adding the dried natural organisms into a medicine crusher, fully crushing, soaking in 70-80% ethanol water solution, and adding a small amount of citric acid, wherein the mass ratio of the natural organisms to the ethanol water solution to the citric acid is 1: 15-25: 0.02-0.05, uniformly mixing the whole system, soaking for 8 hours, and filtering to obtain a natural organism primary extracting solution;

and (3) dialyzing and separating the natural biological primary extract obtained by filtering by using a dialysis membrane with the molecular weight cutoff of 12000-16000 for 2-8 days, carrying out rotary evaporation concentration treatment after dialysis is finished, and then carrying out suction filtration on the whole system through filter paper with the aperture of 1-5 mu m, wherein the pump pressure is 0.02-0.05 MPa, thus obtaining the natural biological extract.

4. The biomass food efficient preservative according to claim 1, characterized in that: the fatty acid ester is one or more of sucrose fatty acid diester, polyglycerol fatty acid ester, ethylhexyl palmitate and propylene glycol fatty acid ester.

5. The biomass food efficient preservative according to claim 1, characterized in that: the particle size range of the allantoin is 25-80 mu m, and the average particle size is 50 mu m.

6. The biomass food efficient preservative according to claim 1, characterized in that: the solvent is a composition of water, ethanol, glycerol and 1, 2-butanediol, wherein the mass ratio of the water to the ethanol to the glycerol is 20: 17: 10: 8.

7. the preparation method of the biomass food high-efficiency preservative according to any one of claims 1 to 6, wherein the preparation method comprises the following steps: the method comprises the specific steps of,

adding 8-16 parts of nisin, 8-16 parts of lysozyme and 6-12 parts of natural biological extract into a container at normal temperature, heating the mixture in a water bath to raise the temperature of the whole system to 35-40 ℃, and stirring and mixing the mixture uniformly at 80-140 rpm;

and continuously and slowly adding 40-70 parts of solvent, 5-10 parts of fatty acid ester and 3-6 parts of allantoin in turn, stirring at the speed of 90-150 rpm while adding, finishing adding within 12-18 min, and adjusting the pH value of the system to be 5.5-7.5 by using citric acid and sodium carbonate to obtain the biomass high-efficiency preservative.

Technical Field

The invention relates to the technical field of food preservatives, and particularly relates to a biomass food efficient preservative and a preparation method thereof.

Background

As for products such as food, medicine, health care products and the like, the safety of the used components is not small and varied, and the components are taken as products needing to be imported, so that the components are not only nontoxic and harmless, but also the safety and health of the products in the shelf life are ensured, and a certain amount of preservative is required to be added to maintain the quality of the products. However, some preservatives interfere with the endocrine function of the human body, have a cumulative effect in the human body and even have certain irritation per se; the preservative has little harm to human bodies but no broad-spectrum sterilization, and the preservative effect is not ideal enough; and many preservatives are not high temperature resistant, and the cleanness and safety of food and medicines are difficult to maintain after temperature change.

In addition, the addition of the preservative may affect the nutrition, properties and efficacy of the food, and the presence of the preservative accounts for the proportion of other effective components.

Therefore, the problem to be solved in the art is to develop a biomass high-efficiency preservative with high-efficiency sterilization and extremely low dosage, greatly reduce or even avoid the influence of the preservative on human health, and enable effective ingredients in food and medicines to better play a role.

Disclosure of Invention

This section is for the purpose of summarizing some aspects of embodiments of the invention and to briefly introduce some preferred embodiments. In this section, as well as in the abstract and the title of the invention of this application, simplifications or omissions may be made to avoid obscuring the purpose of the section, the abstract and the title, and such simplifications or omissions are not intended to limit the scope of the invention.

The present invention has been made in view of the above and/or problems occurring in the existing food preservatives.

Therefore, one of the purposes of the invention is to overcome the defects of the existing food preservative product and provide a biomass food high-efficiency preservative.

To solve the above technical problem, according to an aspect of the present invention, the present invention provides the following technical solutions: the efficient preservative for the biomass food comprises, by mass, 8-16 parts of nisin, 8-16 parts of lysozyme, 6-12 parts of natural biological extract, 5-10 parts of fatty acid ester, 3-6 parts of allantoin and 40-70 parts of solvent.

As a preferable scheme of the high-efficiency preservative for the biomass food, the preservative comprises the following components: the natural biological extract is one or two or more of cactus extract and propolis extract.

As a preferable scheme of the high-efficiency preservative for the biomass food, the preservative comprises the following components: the preparation method of the natural biological extract comprises the steps of drying natural organisms in an oven at 25-35 ℃ for 3-5 hours in advance, adding the dried natural organisms into a medicine crusher, fully crushing, soaking in 70-80% ethanol water solution, and adding a small amount of citric acid, wherein the mass ratio of the natural organisms to the ethanol water solution to the citric acid is 1: 15-25: 0.02-0.05, uniformly mixing the whole system, soaking for 8 hours, and filtering to obtain a natural biological primary extracting solution; and (3) dialyzing and separating the natural biological primary extract obtained by filtering by using a dialysis membrane with the molecular weight cutoff of 12000-16000 for 2-8 days, carrying out rotary evaporation concentration treatment after dialysis is finished, and then carrying out suction filtration on the whole system through filter paper with the aperture of 1-5 mu m, wherein the pump pressure is 0.02-0.05 MPa, thus obtaining the natural biological extract.

As a preferable scheme of the high-efficiency preservative for the biomass food, the preservative comprises the following components: the fatty acid ester is one or more of sucrose fatty acid diester, polyglycerol fatty acid ester, ethylhexyl palmitate and propylene glycol fatty acid ester.

As a preferable scheme of the high-efficiency preservative for the biomass food, the preservative comprises the following components: the particle size range of the allantoin is 25-80 mu m, and the average particle size is 50 mu m.

As a preferable scheme of the high-efficiency preservative for the biomass food, the preservative comprises the following components: the solvent is a composition of water, ethanol, glycerol and 1, 2-butanediol, wherein the mass ratio of the water to the ethanol to the glycerol is 20: 17: 10: 8.

the invention also aims to provide a preparation method of the biomass food high-efficiency preservative.

To solve the above technical problem, according to an aspect of the present invention, the present invention provides the following technical solutions: a preparation method of a biomass food high-efficiency preservative comprises the specific steps of adding 8-16 parts of nisin, 8-16 parts of lysozyme and 6-12 parts of natural biological extract into a container at normal temperature, heating the whole system to 35-40 ℃ in a water bath, and stirring and mixing uniformly at 80-140 rpm; and continuously and slowly adding 40-70 parts of solvent, 5-10 parts of fatty acid ester and 3-6 parts of allantoin in turn, stirring at the speed of 90-150 rpm while adding, finishing adding within 12-18 min, and adjusting the pH value of the system to be 5.5-7.5 by using citric acid and sodium carbonate to obtain the biomass high-efficiency preservative.

The invention has the beneficial effects that:

(1) the biomass high-efficiency preservative adopts biomass raw materials, has obvious effect, can ensure the preservative effect only by using 0.002-0.01 percent of the amount when in use, is non-toxic and has little effective amount, thus having no influence on the health of human bodies and the environment.

(2) The biomass high-efficiency preservative achieves the high-efficiency sterilization and corrosion prevention effects by compounding nisin, lysozyme and natural biological extracts, and experiments prove that the reasonable compounding effect of the three components is far better than that of any one of the three components which are used independently, so that the high-efficiency preservative has high sterilization rate and good sterilization broad spectrum. The two bio-based polypeptides of nisin and lysozyme mainly have killing and inhibiting effects on bacteria and viruses. They break cell walls, break the insoluble mucopolysaccharides of cell walls into soluble glycopeptides, cause the contents of broken cell walls to escape and allow bacterial lysis, and can also directly bind to negatively charged viral proteins, form double salts with DNA, RNA, and apoproteins, and inactivate viruses. The natural biological extract mainly aims at inhibiting mould, and essential oil and volatile components in the natural biological extract have obvious inhibiting and killing effects on the mould. Therefore, the reasonable compounding of the three components greatly improves the sterilization effect and the sterilization broad spectrum of the preservative, and the three components have synergistic effect.

(3) The addition of the allantoin and the fatty acid ester in the biomass high-efficiency preservative not only promotes the sterilization and preservation effects of the biomass high-efficiency preservative, but also has the thickening and emulsifying functions, so that the biomass high-efficiency preservative is particularly suitable for being used in the production and processing of foods, health-care products and the like, the products have ideal properties and excellent preservative performance, the use of other edible additives is reduced, and the total cost is reduced.

(4) The optimal pH application range of the biomass high-efficiency preservative is 5.5-7.5, but the preservative can still have better preservative performance when the pH application range is beyond the optimal pH application range, and the preservative performance can be controlled regularly along with the change of the pH value, namely the preservative has pH value responsiveness.

(5) The biomass high-efficiency preservative has the following surprising findings in the preparation process: the raw materials are added according to the sequence of the solvent in front and the allantoin and the fatty acid ester in back, otherwise the antiseptic performance is obviously reduced, and analysis shows that the addition sequence is more favorable for the exertion of the functions of all the components.

Detailed Description

In order to make the aforementioned objects, features and advantages of the present invention more comprehensible, specific embodiments thereof are described in detail below with reference to examples of the specification.

In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention, but the present invention may be practiced in other ways than those specifically described and will be readily apparent to those of ordinary skill in the art without departing from the spirit of the present invention, and therefore the present invention is not limited to the specific embodiments disclosed below.

Furthermore, reference herein to "one embodiment" or "an embodiment" means that a particular feature, structure, or characteristic described in connection with the embodiment is included in at least one implementation of the invention. The appearances of the phrase "in one embodiment" in various places in the specification are not necessarily all referring to the same embodiment, nor are separate or alternative embodiments mutually exclusive of other embodiments.

Nisin (nisin) used in the present invention, commercially available, food additive, luoyang qihong biotechnology limited; lysozyme, commercially available, food grade, nanning pombo bioengineering, ltd; other raw materials, which are not specially indicated, are all commercially available and are food grade.

The test method comprises the following steps:

and (3) testing the corrosion resistance: the preservative was tested in a fluid food product at 0.005% based on CTFA challenge test and evaluation criteria, with an initial inoculum size of 10⁶cfu/g (note: no other ingredients in the food sample that contribute to sterilization, bacteriostasis, preservation), then:

at 28 days, the samples contain bacteria or mould of more than 1000cfu/g, the samples can not pass the bacteria-adding antiseptic challenge test, the antiseptic in the samples can not effectively inhibit the action of microorganism, and the products are easily polluted by the microorganism during production, storage and use.

On day 28, the sample contains 100-1000 cfu/g of bacteria or mold, and the sample conditionally passes the challenge test, namely the protein or other animal and biological material components in the sample are not particularly high, the produced sanitary environment meets the requirement, and the preservative can be used when the package is not easy to generate secondary pollution, otherwise, the preservative cannot be used.

On the 28 th day, the sample contains 10-100 cfu/g of bacteria or mould, and the challenge test of the sample shows that the preservative in the sample has a strong effect of inhibiting and killing microorganisms, so that the product is not easily polluted by the microorganisms during production, storage and use.

From day 7 onwards, the samples contained <10cfu/g of bacteria or mold, and the samples passed challenge tests, which indicated that the preservatives in the samples had a particularly strong killing effect on microorganisms, and the products were not easily contaminated with microorganisms during production, storage and use.