阅读说明：本技术 一种巴戟天提取物在制备抗骨骼肌疲劳剂中的应用 (Application of morinda officinalis extract in preparation of anti-skeletal muscle fatigue agent ) 是由 赵玉勤 于 2019-10-14 设计创作，主要内容包括：本发明涉及中药提取物及其应用领域,公开了一种巴戟天提取物在制备抗骨骼肌疲劳剂中的应用,抗骨骼肌疲劳剂的组分包括巴戟天提取物0.1～2.5mg/mL、NaCl 6～7mg/mL,KCl 0.13～0.15mg/mL,NaHCO<Sub>3</Sub> 0.1～0.3mg/mL,CaCl<Sub>2</Sub> 0.1～0.3mg/mL,NaH<Sub>2</Sub>PO<Sub>4</Sub> 0.01～0.02 mg/mL,葡萄糖1～3mg/mL和水。本发明用传统中草药巴戟天提取物作为抗骨骼肌疲劳剂中的主要成分,可以使骨骼肌达到疲劳状态的时间延长,有效提高骨骼肌的抗疲劳活性,并且无需添加兴奋剂,是一种安全高效的抗疲劳药物。(The invention relates to the field of traditional Chinese medicine extracts and application thereof, and discloses application of a morinda officinalis extract in preparation of an anti-skeletal muscle fatigue agent, wherein the anti-skeletal muscle fatigue agent comprises the components of 0.1-2.5 mg/mL of the morinda officinalis extract, 6-7 mg/mL of NaCl, 0.13-0.15 mg/mL of KCl and NaHCO 3 0.1～0.3mg/mL，CaCl 2 0.1～0.3mg/mL，NaH 2 PO 4 0.01-0.02 mg/mL, 1-3 mg/mL glucose and water. The invention uses traditional Chinese herbal medicine morinda officinalis extract as the main component of the anti-fatigue agent for skeletal muscles, can prolong the time for the skeletal muscles to reach a fatigue state, effectively improves the anti-fatigue activity of the skeletal muscles, does not need to add an excitant, and is a safe and efficient anti-fatigue drug.)

1. The application of the morinda officinalis extract in preparing the skeletal muscle fatigue resisting agent is characterized in that the components of the skeletal muscle fatigue resisting agent comprise the morinda officinalis extract, NaCl, KCl and NaHCO₃、CaCl₂、NaH₂PO₄Glucose and water.

2. The use of Morinda citrifolia extract in the preparation of an anti-skeletal muscle fatigue agent according to claim 1, wherein the concentration of Morinda citrifolia extract in the anti-skeletal muscle fatigue agent is 0.1-2.5 mg/mL.

3. The use of Morinda citrifolia extract in the preparation of an anti-skeletal muscle fatigue agent according to claim 1 or 2, wherein the anti-skeletal muscle fatigue agent comprises the following ingredients: 6-7 mg/mL NaCl, 0.13-0.15 mg/mL KCl, NaHCO₃0.1～0.3mg/mL，CaCl₂0.1～0.3mg/mL，NaH₂PO₄0.01-0.02 mg/mL, and 1-3 mg/mL glucose.

4. The use of Morinda citrifolia extract to prepare an anti-skeletal muscle fatigue agent according to claim 1, wherein said Morinda citrifolia extract is prepared by the steps of:

(1) crude extraction: adding water into radix Morindae officinalis powder, ultrasonically extracting, filtering to obtain primary filtrate and primary residue, extracting the primary residue with the same method for three times, mixing the three filtrates with the primary filtrate, concentrating, and drying to obtain crude extract;

(2) fine extraction: adding ethanol into the crude extract of morinda officinalis, precipitating with ethanol, filtering to obtain a secondary filtrate, concentrating the secondary filtrate, and drying to obtain the extract of morinda officinalis.

5. The use of the extract of morinda officinalis how to prepare an anti-skeletal muscle fatigue agent as claimed in claim 4, wherein the mass ratio of morinda officinalis powder to water in step (1) is 1: (5-10).

6. The use of the Morinda citrifolia extract in preparing an anti-skeletal muscle fatigue agent according to claim 4, wherein the ultrasonic power in step (1) is 800-1000W, the frequency is 40-60 Hz, and the extraction time is 20-40 min.

7. The use of Morinda citrifolia extract for preparing an anti-fatigue agent for skeletal muscle according to claim 4, wherein the filtration in step (1) and step (2) is performed using filter paper with a pore size of 30-50 μm.

8. The use of Morinda citrifolia extract for the preparation of an anti-skeletal muscle fatigue agent according to claim 4, wherein the weight ratio of Morinda citrifolia crude extract to ethanol in step (2) is 2: (3-5).

9. The use of the Morinda citrifolia extract in the preparation of an anti-fatigue agent for skeletal muscle according to claim 4, wherein the alcohol precipitation time in step (2) is 6-12 h.

Technical Field

The invention relates to the field of traditional Chinese medicine extracts and application thereof, in particular to application of a morinda officinalis extract in preparation of an anti-skeletal muscle fatigue agent.

Background

In modern society, as various external stresses and psychological stresses are continuously increased, various age groups begin to show different levels of fatigue. The sleepiness symptom is slightly appeared, but the sleepiness symptom can be relieved by supplementing nutrition, resting and sleeping and the like; endocrine dyscrasia, organ pathological changes and even mental diseases appear seriously, and the physical and mental health of people is threatened seriously. Along with the development of science, various researches on fatigue are continuously deepened, and anti-fatigue medicines are also continuously generated. However, some anti-fatigue drugs contain components such as stimulants, which may cause certain harm to the body, so the application of the anti-fatigue drugs is also greatly limited.

Efforts are also underway to develop safer, effective anti-fatigue drugs. The traditional Chinese herbal medicine in China is the focus of research and development of the medicine in recent years because of the advantages of few side effects and no hidden danger caused by adding an excitant. Morinda officinalis (Morinda officinalis) is a traditional Chinese herbal medicine in China, is still widely applied to the field of traditional Chinese medicine at present, and is mainly used for strengthening bones and muscles, dispelling wind-damp, tonifying qi and blood and the like. In addition, the morinda officinalis is also rich in saccharides, anthraquinones, iridoids, flavonoids and other chemical components through detection, and modern pharmacological research also shows that due to the diversity of functions of the morinda officinalis, the morinda officinalis is not limited to the traditional Chinese medicine effects, and has certain auxiliary effects on resisting aging, enhancing the immunity of a human body, resisting myocardial anoxia and resisting cancers. For example, the application of morinda officinalis extract in the preparation of immunoadjuvant disclosed in chinese patent document, whose publication No. CN103372208B, discloses that morinda officinalis extract can be used as a main effective ingredient for preparing immunoadjuvant, i.e., morinda officinalis extract can be used for preparing immunoadjuvant, which is a safe and effective vaccine adjuvant capable of enhancing antigen immunoprotection effect. Can be applied to the immune protection of the traditional attenuated vaccine, the inactivated vaccine, the DNA vaccine, the polypeptide vaccine and the inactivated protein vaccine.

However, the application of the morinda officinalis extract in the anti-fatigue medicine is not available in the existing research, and if the effective components in the morinda officinalis can be extracted and used for preparing the anti-fatigue medicine, the side effects of the anti-fatigue medicine can be greatly reduced, so that the anti-fatigue medicine is safer and has extremely high application value and economic development value.

Disclosure of Invention

The invention aims to overcome the problems that the anti-fatigue medicine in the prior art contains components such as stimulant and the like, and causes certain harm to the organism after being taken, and needs to find a safe and efficient anti-fatigue medicine with less side effect and no stimulant, and provides the application of the morinda officinalis extract in preparing the anti-skeletal muscle fatigue agent.

In order to achieve the purpose, the invention adopts the following technical scheme:

application of radix Morindae officinalis extract in preparing anti-skeletal muscle fatigue agent comprises radix Morindae officinalis extract, NaCl, KCl, and NaHCO₃、CaCl₂、NaH₂PO₄Glucose and water.

Preferably, the concentration of the morinda officinalis extract in the anti-skeletal muscle fatigue agent is 0.1-2.5 mg/mL.

Preferably, the concentration of each component in the anti-skeletal muscle fatigue agent is as follows: 6-7 mg/mL NaCl, 0.13-0.15 mg/mL KCl, NaHCO₃0.1～0.3mg/mL，CaCl₂0.1～0.3mg/mL，NaH₂PO₄0.01-0.02 mg/mL, and 1-3 mg/mL glucose.

Preferably, the preparation method of the morinda officinalis extract comprises the following steps:

(1) crude extraction: adding water into radix Morindae officinalis powder, ultrasonically extracting, filtering to obtain primary filtrate and primary residue, extracting the primary residue with the same method for three times, mixing the three filtrates with the primary filtrate, concentrating, and drying to obtain crude extract;

(2) fine extraction: adding ethanol into the crude extract of morinda officinalis, precipitating with ethanol, filtering to obtain a secondary filtrate, concentrating the secondary filtrate, and drying to obtain the extract of morinda officinalis.

Preferably, in the step (1), the mass ratio of the morinda officinalis medicinal material powder to the water is 1: (5-10).

Preferably, the ultrasonic power in the step (1) is 800-1000W, the frequency is 40-60 Hz, and the extraction time is 20-40 min.

Preferably, filter paper having a pore size of 30 to 50 μm is used for the filtration in the step (1) and the step (2).

Preferably, the mass ratio of the morinda officinalis crude extract to the ethanol in the step (2) is 2: (3-5).

Preferably, the alcohol precipitation time in the step (2) is 6-12 h.

Fatigue is classified into peripheral nervous fatigue and central fatigue. There are many factors that can cause peripheral neuropathic fatigue, and most of the prior studies have been concerned with the energy required for muscle contraction. All energy required by our daily life is provided by three substances, namely, carbohydrates, lipids and proteins. Exercise is accompanied by energy consumption, and these substances are also greatly reduced, and if not timely supplemented, fatigue will be caused. The saccharides in the three are also the main energy substances for maintaining the life activities of human bodies, and are always preferentially utilized, so that the blood sugar level is reduced, and the fatigue is followed.

One of the factors causing central fatigue is that oxidative reactions occurring in the brain cause damage to the brain, and in order to prevent such damage, the brain makes a corresponding defense mechanism, thereby causing fatigue. During daily physical exercise, the content of active oxygen in the brain increases, further promoting the occurrence of oxidation reaction. For organs that are highly sensitive to damage by reactive oxygen species, the number of oxidizable molecules determines the extent of damage.

In addition, fatigue is caused by peroxidation of free radicals and lipid, and the free radicals are substances which are generated in the oxidation reaction in organisms and cause damage to organisms, are accompanied by strong oxidizability, and can oxidize various substances in the organisms and change the configuration of compounds. The free radical oxidation reaction may occur because the active oxygen radicals combine with unsaturated fatty acids on the plasma membrane of cells to cause lipid peroxidation, lipid peroxides are generated, and then the lipid peroxides are continuously degraded into terminal products such as Malondialdehyde (MDA) and alkanes, and the like, and the products are accumulated to damage the cell membrane, finally the cell is damaged to die, and various pathological changes are caused. When the reaction of in vivo free radical oxidation to destroy cell membrane occurs, the enzyme Catalase (CAT) capable of specifically combining with the oxidizing substance is generated in human body, and the amount of the enzyme is greatly increased, so as to achieve the purpose of antioxidation. Furthermore, Catalase (CAT) is an important antioxidant enzyme that scavenges oxygen radicals in the body and prevents damage to cellular structures. It is known that free radicals produced by skeletal muscle movement cause changes in CAT activity, and therefore the magnitude of CAT activity is closely related to skeletal muscle fatigue.

The morinda officinalis extract obtained by the method contains various anthraquinones and iridoid compounds, and the mother ring of the iridoid compounds contains carbon-carbon double bonds which have chemical properties easy to oxidize, so that the compounds have certain antioxidant activity; in addition, the morinda officinalis extract also contains a plurality of soluble polysaccharides which are used as one of main energy supply substances for life activities, saccharides play a very important role in maintaining normal life activities of the organism, and the energy supply of the saccharides is also required when skeletal muscles contract; polysaccharide compounds are the largest number of natural macromolecules, can be used as protective agents to resist oxidation reaction, can be used as a retardant for aging reaction, and can change nutrient metabolism, release intestinal hormone, regulate immune system and intestinal microorganisms; in addition, the invention adds K into the anti-skeletal muscle fatigue agent⁺、Ca²⁺、Na⁺And inorganic ions, which are closely related to the contraction of skeletal muscle.

Therefore, the morinda officinalis extract prepared by the method is applied to the anti-skeletal muscle fatigue agent in a proper concentration, so that the time for the skeletal muscle to reach a fatigue state can be prolonged, and the anti-fatigue activity of the skeletal muscle can be effectively improved.

Therefore, the invention has the following beneficial effects:

(1) the extract of morinda officinalis which is a traditional Chinese herbal medicine is used as a main component of the anti-fatigue agent for skeletal muscles, so that the time for the skeletal muscles to reach a fatigue state can be prolonged, the anti-fatigue activity of the skeletal muscles is effectively improved, and an excitant is not required to be added, so that the anti-fatigue agent is a safe and efficient anti-fatigue medicine;

(2) the morinda officinalis extract obtained by the method contains various anthraquinones, iridoid compounds and polysaccharides, and is assisted by antibioticsK in skeletal muscle fatigue agent⁺、Ca²⁺、Na⁺And the like, has certain antioxidant activity, can supply energy for the contraction of skeletal muscles, is used as a main component in an anti-skeletal muscle fatigue agent, and can effectively relieve skeletal muscle fatigue.

Drawings

FIG. 1 is an IR spectrum of Morinda citrifolia extract of example 1;

FIG. 2 is a high performance liquid chromatogram of Morinda citrifolia extract of example 1;

FIG. 3 is a high performance liquid chromatogram of a standard sample;

FIG. 4 is a graph showing the effect of the anti-skeletal muscle fatigue agent of examples 1 to 3 on the calf contraction force of a toad.

Detailed Description

The invention is further described with reference to the following detailed description and accompanying drawings.