阅读说明：本技术 使用柑橘衰退病毒载体进行外源基因表达 (Exogenous gene expression using citrus tristeza virus vectors ) 是由 威廉·欧·多森 斯维特拉娜·弗里莫诺娃 卓阿·埃尔默塔 于 2012-09-21 设计创作，主要内容包括：本发明揭示了基于对柑橘衰退病毒的修改、为有益目的用于转染柑橘树的病毒载体。本发明揭示了包括含有一个或多个编码异源性多肽的基因盒的病毒载体。所述基因盒位于病毒基因组上的理想位点,使得增加表达量的同时保存病毒的功能性。本发明同时揭示了用病毒载体转染植物的方法以及被病毒转染的植物的实施例。(The present invention discloses a viral vector comprising or more gene cassettes encoding heterologous polypeptides, said gene cassettes being located at desired sites on the viral genome such that expression is increased while preserving the functionality of the virus.)

CTV viral vectors for infecting citrus trees, said CTV viral vectors being constructed to contain at least gene cassettes comprising a heterologous nucleic acid, said CTV viral vectors being constructed such that said gene cassettes are located between the CTV genomic regions p13-p20 or p20-p23, and said heterologous nucleic acid is expressed when the citrus trees are infected.

2. The CTV viral vector as claimed in claim 1, wherein the at least gene cassettes are located between CTV genomes p13-p 20.

3. The CTV viral vector as claimed in claim 1, wherein the at least gene cassettes are located between CTV genomes p20-p 23.

4. The CTV viral vector as recited in claim 1 wherein said at least gene cassettes further comprises steps comprising at least subgenomic mRNA control subelements upstream of said polynucleotide encoding a heterologous polypeptide.

A method of infecting trees to express a heterologous polypeptide, the method comprising transfecting at least cells of a tree with a CTV viral vector configured to comprise at least gene cassettes comprising a polynucleotide encoding a heterologous polypeptide, the CTV viral vector configured to have the gene cassettes located between the p13-p20 or p20-p23 regions of the CTV genome and to be expressed when a tree is infected.

6. The method as recited in claim 5, wherein said tree is a citrus tree.

7. The CTV viral vector as recited in claim 1 wherein said gene cassette further comprises steps of subgenomic mRNA control subelements located upstream of the polynucleotide encoding the heterologous polypeptide.

8. The vector of claim 1, wherein the CTV viral vector is constructed to comprise a plurality of gene cassettes at or more positions.

9. The vector of claim 8, wherein the CTV viral vector comprises at least two gene cassettes at sites and at least gene cassettes at different sites.

10. The method of claim 5, wherein the CTV viral vector is constructed to comprise a plurality of gene cassettes at or more positions.

11. The vector of claim 8, wherein the CTV viral vector comprises at least two gene cassettes at sites and at least gene cassettes at different sites.