阅读说明：本技术 一种具有生精作用的药物组合物 (A pharmaceutical composition with vital essence generation effect ) 是由 李世杰 刘涛 宋卓悦 叶贤英 陈瑶 于 2019-10-28 设计创作，主要内容包括：本发明涉及一种具有生精作用的药物组合物,该药物组合物由有效成分和医学上可接受的辅料组成,其特征在于,所述的有效成分为以下重量百分比原料药制备的分子量大于3500道尔顿的多糖提取物：牡蛎肉干品37～55％,巴戟天23～35％,肉苁蓉14～22％,刺参6～8％。本发明所述药物组合物具有药简力专,效果显著的优点。(The invention relates to a pharmaceutical composition with spermatogenic effect, which consists of effective components and medically acceptable auxiliary materials, and is characterized in that the effective components are polysaccharide extracts with molecular weight more than 3500 daltons, which are prepared from the following raw material medicines in percentage by weight: 37-55% of dried oyster, 23-35% of morinda officinalis, 14-22% of cistanche and 6-8% of stichopus japonicus. The pharmaceutical composition has the advantages of simple and special medicine and remarkable effect.)

1. A pharmaceutical composition with spermatogenic effect comprises effective components and medically acceptable auxiliary materials, and is characterized in that the effective components are polysaccharide extracts with molecular weight more than 3500 daltons, which are prepared from the following raw material medicines in percentage by weight:

37-55% of dried oyster, 23-35% of morinda officinalis, 14-22% of cistanche and 6-8% of stichopus japonicus.

2. The pharmaceutical composition with spermatogenic effect according to claim 1, wherein the effective components are polysaccharide extracts with molecular weight greater than 3500 daltons prepared from the following bulk drugs in percentage by weight:

46% of dried oyster, 29% of morinda officinalis, 18% of cistanche and 7% of stichopus japonicus.

3. The composition of claim 1 or 2, wherein the polysaccharide extract is prepared by the following method:

(1) taking the raw materials, drying, crushing, adding 15-25 times of hot water with the temperature of 75-80 ℃, ultrasonically extracting for two times, each time for 20-30 minutes, collecting an extracting solution, and concentrating into an extract;

(2) adding ethanol with the volume of 4 times of the obtained extract for precipitation, collecting precipitate, centrifuging, and freeze-drying to obtain freeze-dried powder;

(3) dissolving the obtained lyophilized powder in water, dialyzing with dialysis bag with molecular weight cutoff of 3500 Dalton, collecting polysaccharide dialysate with molecular weight greater than 3500 Dalton, concentrating, and lyophilizing to obtain the polysaccharide extract.

Technical Field

The invention relates to a medical preparation, in particular to a medicinal preparation containing undetermined structures of traditional Chinese medicine raw materials, which has the effect of promoting vital essence generation.

Background

Infertility is a common clinical problem affecting nearly 15% of couples worldwide. Approximately 50% of infertility factors are caused by male factors including oxidative stress damage, diabetes-induced hyperglycemia damage, DNA damage, chronic intermittent thermal stress, and copper exposure, among others. Oxidative stress injury has now been identified as an important factor in the development of reproductive dysfunction leading to male infertility. Oxidative stress causes atrophy and reduction of the number of spermatogenic cells at all levels in the male testis, the number of mature sperms is reduced, and most of the mature sperms are abnormal.

At present, vitamin E is the main clinical medicine for male oxidative stress reproductive injury, but the long-term taking of the medicine can cause hypertension, atherosclerosis, aggravation of coronary heart disease and angina pectoris [ Zhao Hai Ying, study on side effect of vitamin E [ J ]. strait pharmacy 2005.17(4): 155-. Chinese patent medicine with less toxic and side effects is also commonly used for male reproductive injury, but the prescription is complex, for example, the cynomorium songaricum semen-securing pill is composed of 24 Chinese medicines such as cynomorium songaricum, cistanche, morinda officinalis and the like, and is not beneficial to quality control. Patent application with publication number CN109043105A discloses a cordyceps and sea cucumber peptide tablet candy for tonifying kidney and producing sperm, which is prepared from 5 traditional Chinese medicines such as cordyceps polysaccharide compound, sea cucumber peptide, rhizoma polygonati, oyster peptide, medlar and the like. Although the medicine can achieve the purposes of nourishing yin, tonifying kidney, producing sperm and enhancing the kidney function of human body, the main medicine of the medicine is precious cordyceps, the cost is high, the extraction and preparation process is complicated, the production period is long, and the production and market popularization are not facilitated.

Therefore, the search for a suitable medicament for improving the oxidative stress injury of male reproduction is a clinically urgent problem to be solved.

Disclosure of Invention

The technical problem to be solved by the invention is to provide the pharmaceutical composition with the function of promoting vital essence generation, and the pharmaceutical composition has the advantages of simple and special medicament and remarkable effect.

The technical scheme for solving the problems is as follows:

a pharmaceutical composition with spermatogenic effect comprises effective components and medically acceptable auxiliary materials, and is characterized in that the effective components are polysaccharide extracts with molecular weight more than 3500 daltons, which are prepared from the following raw material medicines in percentage by weight: 37-55% of dried oyster, 23-35% of morinda officinalis, 14-22% of cistanche and 6-8% of stichopus japonicus.

In the scheme, the optimal mixture ratio of the raw material medicines is as follows: 46% of dried oyster, 29% of morinda officinalis, 18% of cistanche and 7% of stichopus japonicus.

In the above scheme, the dried Carnis Ostreae product can be prepared by removing shell of Ostrea rivularis, Ostrea gigas, and Ostrea denselamellosa of Ostreidae, collecting meat, and sun drying (see related records in Chinese materia Medica).

In the scheme, the polysaccharide extract is prepared by the following method:

(1) taking the raw materials, drying, crushing, adding 15-25 times of hot water with the temperature of 75-80 ℃, ultrasonically extracting for two times, each time for 20-30 minutes, collecting an extracting solution, and concentrating into an extract;

(2) adding ethanol with the volume of 4 times of the obtained extract for precipitation, collecting precipitate, centrifuging, and freeze-drying to obtain freeze-dried powder;

(3) dissolving the obtained lyophilized powder in water, dialyzing with dialysis bag with molecular weight cutoff of 3500 Dalton, collecting polysaccharide dialysate with molecular weight greater than 3500 Dalton, concentrating, and lyophilizing to obtain the polysaccharide extract.

The pharmaceutical composition provided by the invention is a common oral preparation, such as granules, tablets or capsules.

The pharmaceutical composition has obvious improvement effect on the spermatogenic ability of oxidative stress injury mice caused by cyclophosphamide, and can be used for treating diseases related to spermatogenic dysfunction. The diseases related to spermatogenic dysfunction comprise: testicular atrophy, oligospermia, asthenospermia.

Detailed Description

The present invention will be described in detail with reference to specific examples.