阅读说明：本技术 一种利用贻贝废弃物质制备高蛋白高钙补钙剂的方法 (Method for preparing high-protein high-calcium supplement by using mussel waste materials ) 是由 纪丽丽 张岩 王亚宁 宋文东 郭健 蔡璐 于 2019-09-30 设计创作，主要内容包括：本发明涉及添加剂加工,特别是涉及一种利用贻贝废弃物质制备高蛋白高钙补钙剂的方法,包括1)取贻贝壳洗净酸浸,蒸馏水清洗至清洗液呈中性后粉碎成微纳米贻贝壳粉；2)贻贝去壳去内脏后洗净晾干,捣碎后加蒸馏水蒸煮,取贻贝蒸煮汁上清液；3)贻贝蒸煮汁上清液依次经胰蛋白酶水解与氨肽酶水解,得酶解液；4)酶解液与贻贝壳粉完成螯和反应,过滤掉反应残渣,反应溶液进行干燥即得。本制备方法简单易行,实现了废弃物资源回收利用,避免了营养成分的流失,提升了经济价值,补钙剂的总钙含量、总蛋白含量以及氨基酸液态氮含量较高,易于吸收,充分利用贻贝的独特风味,利于促进禽、畜、鱼等对高蛋白高钙补钙剂的高效摄入与吸收。(The invention relates to additive processing, in particular to a method for preparing a high-protein high-calcium supplement by using mussel waste materials, which comprises the following steps of 1) taking mussel shells, cleaning, pickling, cleaning with distilled water until the cleaning solution is neutral, and crushing into micro-nano mussel shell powder; 2) removing shells and viscera of mussels, cleaning, air drying, mashing, adding distilled water, and decocting to obtain supernatant of decoction; 3) sequentially hydrolyzing the supernatant of the mussel decoction by trypsin and aminopeptidase to obtain an enzymatic hydrolysate; 4) and (3) carrying out chelation reaction on the enzymatic hydrolysate and the mussel shell powder, filtering reaction residues, and drying the reaction solution to obtain the compound. The preparation method is simple and easy to implement, realizes the recycling of waste resources, avoids the loss of nutrient components, improves the economic value, has higher total calcium content, total protein content and amino acid liquid nitrogen content of the calcium supplement, is easy to absorb, fully utilizes the unique flavor of mussels, and is beneficial to promoting the high-efficiency intake and absorption of high-protein high-calcium supplements for poultry, livestock, fish and the like.)

1. A method for preparing a high-protein high-calcium supplement by using mussel waste materials is characterized by comprising the following steps:

1) cleaning mussel shells, soaking the mussel shells in 1-2 mol/L hydrochloric acid for 5-6 h, cleaning the mussel shells with distilled water until the cleaning solution is neutral, and crushing the mussel shells into micro-nano mussel shell powder by using a micro-nano crusher;

2) removing shells and viscera of mussels, cleaning, air drying, mashing, adding distilled water, and decocting to obtain supernatant of decoction;

3) sequentially hydrolyzing the supernatant of the mussel decoction by trypsin and aminopeptidase to obtain an enzymolysis solution containing proteins with molecular weight of below 500 Da;

4) the enzymolysis liquid and the mussel shell powder complete the chelation reaction, the reaction residue is filtered, and the reaction solution is dried to obtain the high-protein high-calcium supplement.

2. The method of claim 1, wherein: in high-protein high-calcium supplement

The total protein content can reach more than 16.8 percent,

the total calcium content is not less than 60 percent,

the content of amino acid liquid nitrogen is not less than 0.86g/100 mL.

3. The method according to claim 1 or 2, characterized in that: the particle size of the micro-nano mussel shell powder obtained in the step 1) is 1-2 nm.

4. The method according to claim 1 or 2, characterized in that: m is used for cooking in the step 2)_Mussel:m_{Water (W)}Cooking for 1h at the ratio of 1: 1-2.

5. The method according to claim 1 or 2, characterized in that: the trypsin hydrolysis method of the step 3) comprises the following steps: adding 3-5% of trypsin, and carrying out enzymolysis for 2-3 h at the pH of 8.0-10 and the temperature of 40-50 ℃.

6. The method according to claim 1 or 2, characterized in that: the aminopeptidase hydrolysis method of the step 3) comprises the following steps: adding 4% aminopeptidase, and performing enzymolysis at 55 deg.C and pH9.0 for 2.5 h.

7. The method according to claim 1 or 2, characterized in that: in the chelation reaction in the step 4), the material-liquid ratio is 1: 2-4 (g/g), the temperature is 40 ℃, and the chelation reaction is 60-90 min.

8. The method according to claim 1 or 2, characterized in that: the drying of step 4) may be selected from spray drying.

9. A high-protein high-calcium supplement, which is prepared by the method for preparing the high-protein high-calcium supplement by using mussel waste materials according to any one of claims 1 to 8.

10. The use of the high protein high calcium supplement of claim 9, wherein said use comprises:

1) the application in the rapid calcium supplement of the cultured animals; and/or

2) Use in the prevention and/or treatment of calcium deficiency in farmed animals; and/or

3) The application of the composition in improving the immunity and/or protecting the liver function of the cultured animals; and/or

4) The use in the prevention and/or treatment of acidosis in farmed animals; and/or

5) Use in maintaining electrolyte balance in a farmed animal; and/or

6) The application in smoothly shelling and fixing shells of shell-changing animals.

Technical Field

The invention relates to the field of additive processing, in particular to a method for preparing a high-protein high-calcium supplement by using mussel waste materials.

Background

The shellfish production capacity in China is the first in the world, mussels are the main production target, and due to the demand, wild fishing and a plurality of artificial breeding plants exist. In addition to eating the soft part of mussel, people can also eat a plurality of processing methods, such as mussel meat cans, mussel flavor spices and the like. For ease of transportation and to extend the shelf life, the more common processed products are frozen and dried products. In this course of working, produce a large amount of mussel boiling water, many producers go the mussel boiling water and directly discharge in the river, cause a large amount of wastes, also caused huge harm to the environment. Except for mussel cooking juice, mussel shells are the most basic waste, and according to the demand of China for mussels, huge quantities of mussel shells can be generated every year, and are stacked up to cause huge damage to the environment. Mussel cooking juice is milk white and contains a large amount of protein, free amino acid, liver sugar and the like, mussel shells contain rich calcium, trace elements and organic matters which are not fully utilized to cause a large amount of nutrition loss, and the substances are just necessary nutrient substances for growth of poultry, livestock, fish and the like. Therefore, the invention utilizes the mussel boiling juice and the mussel shells to prepare a high-protein high-calcium supplement, supplements trace elements such as calcium and the like, and simultaneously supplements the amino acid content in the body.

The above background disclosure is only for the purpose of assisting understanding of the inventive concept and technical solutions of the present invention, and does not necessarily belong to the prior art of the present patent application, and should not be used for evaluating the novelty and inventive step of the present application in the case that there is no clear evidence that the above content is disclosed at the filing date of the present patent application.

Disclosure of Invention

The invention aims to provide a method for preparing a high-protein high-calcium supplement by using mussel waste, which is simple, realizes the recycling of waste resources, avoids the loss of nutrient components, improves the economic value, has higher total calcium content, total protein content and amino acid liquid nitrogen content, is easy to absorb, fully utilizes the unique flavor of mussels, and is beneficial to promoting the efficient absorption and absorption of poultry, livestock, fish and the like on the high-protein high-calcium supplement.

The technical scheme adopted by the invention comprises the following aspects.

In a first aspect, the present application provides a method for preparing a high-protein high-calcium supplement by using mussel waste material, which comprises the following steps:

1) cleaning mussel shells, soaking the mussel shells in 1-2 mol/L hydrochloric acid for 5-6 h, cleaning the mussel shells with distilled water until the cleaning solution is neutral, and crushing the mussel shells into micro-nano mussel shell powder by using a micro-nano crusher;

2) removing shells and viscera of mussels, cleaning, air drying, mashing, adding distilled water, and decocting to obtain supernatant of decoction;

3) sequentially hydrolyzing the supernatant of the mussel decoction by trypsin and aminopeptidase to obtain an enzymolysis solution containing proteins with molecular weight of below 500 Da;

4) the enzymolysis liquid and the mussel shell powder complete the chelation reaction, the reaction residue is filtered, and the reaction solution is dried to obtain the high-protein high-calcium supplement.

Preferably, the particle size of the micro-nano mussel shell powder obtained in the step 1) is 1-2 nm.

Preferably, the cooking of step 2) is performed in m_Mussel:m_{Water (W)}Cooking for 1h at the ratio of 1: 1-2.

Preferably, the trypsin hydrolysis method of step 3) is: adding 3-5% of trypsin, and carrying out enzymolysis for 2-3 h at the pH of 8.0-10 and the temperature of 40-50 ℃.

Preferably, the aminopeptidase hydrolysis method of step 3) is: adding 4% aminopeptidase, and performing enzymolysis at 55 deg.C and pH9.0 for 2.5 h.

Preferably, in the chelation reaction in the step 4), the material-liquid ratio is 1: 2-4 (g/g), the temperature is 40 ℃, and the chelation reaction is 60-90 min.

Preferably, the drying of step 4) may be selected from spray drying.

The preparation method utilizes mussel waste mussel cooking juice and mussel shell chela to prepare the high-protein high-calcium supplement, and the high-protein high-calcium supplement prepared by the preparation method has the advantages that the total protein content can reach more than 16.8%, the total calcium content is not less than 60%, the amino acid liquid nitrogen content is not less than 0.86g/100mL, calcium ions can be slowly released to be absorbed by a group, the molecular structure of the calcium supplement is similar to that of low-peptide protein calcium in a natural substance and is easy to be absorbed by intestinal tracts, on one hand, mussel resources are fully utilized, the recycling of waste resources is realized, the full utilization of calcium of residual nutrients in the mussel cooking juice and mussel shells is creatively realized, the loss of nutrient components is avoided, the economic value is improved, the environment friendliness is achieved, on the other hand, the defect of single flavor of the traditional calcium supplement is overcome, the unique flavor of mussels is fully utilized, and the promotion of poultry and, The high-protein high-calcium supplement is efficiently ingested and absorbed by livestock, fish and the like, so that the amino acid content in the body is fully supplemented, and the problem that the calcium supplement mode of food supplement cannot meet the calcium supplement requirement of the body is solved.

In a second aspect, the present application further provides a high-protein high-calcium supplement, which is prepared by any one of the methods described in the first aspect above for preparing a high-protein high-calcium supplement from mussel waste.

In a third aspect, the present application also provides a use of the high protein and high calcium supplement of the second aspect, including but not limited to:

1) the application in the rapid calcium supplement of the cultured animals; and/or

2) Use in the prevention and/or treatment of calcium deficiency in farmed animals; and/or

3) The application of the composition in improving the immunity and/or protecting the liver function of the cultured animals; and/or

4) The use in the prevention and/or treatment of acidosis in farmed animals; and/or

5) Use in maintaining electrolyte balance in a farmed animal; and/or

6) The application in smoothly shelling and fixing shells of shell-changing animals.

The invention has the beneficial effects that:

1) the high-protein high-calcium supplement prepared by the method has the advantages that the total protein content can reach more than 16.8%, the total calcium content is not lower than 60%, the content of amino acid liquid nitrogen is not lower than 0.86g/100mL, calcium ions can be slowly released for collective absorption, the molecular structure of the calcium supplement is similar to that of low-peptide protein calcium in natural real objects, and the calcium supplement is easy to absorb by intestinal tracts;

2) mussel resources are fully utilized, waste resource recycling is realized, calcium of residual nutrients in mussel cooking juice and mussel shells is creatively and fully utilized, loss of nutrient components is avoided, economic value is improved, and the method is environment-friendly;

3) the defect of single flavor of the traditional calcium supplement is overcome, the unique flavor of the mussel is fully utilized, the high-protein high-calcium supplement is promoted to be efficiently absorbed by poultry, livestock, fish and the like, the amino acid content in the body is fully supplemented, and the problem that the calcium supplement mode of food supplement cannot meet the calcium supplement requirement of the body is solved.

The invention adopts the technical scheme to provide the model essay, makes up the defects of the prior art, and has reasonable design and convenient operation.

Detailed Description

Unless defined otherwise, technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The present invention uses the methods and materials described herein; other suitable methods and materials known in the art may be used. The materials, methods, and examples described herein are illustrative only and are not intended to be limiting. All publications, patent applications, patents, provisional applications, database entries, and other references mentioned herein, and the like, are incorporated by reference herein in their entirety. In case of conflict, the present specification, including definitions, will control.