阅读说明：本技术 一种治疗腹泻的四联活菌制剂及其应用 (Quadruple viable bacteria preparation for treating diarrhea and application thereof ) 是由 德艳艳 廖洪 张艳花 于 2019-10-22 设计创作，主要内容包括：本发明公开了一种治疗腹泻的四联活菌制剂及其应用,包括5-50％益生菌混合物、6-10％低聚木糖,余量为葡萄糖,益生菌混合物包括动物双歧杆菌冻干粉、副干酪乳酸杆菌冻干粉、屎肠球菌冻干粉和酿酒酵母菌冻干粉。本发明的双歧杆菌、乳酸杆菌、屎肠球菌和酿酒酵母菌复合活菌制剂用于治疗犬肠道菌群失调引起腹泻,该制品对犬肠道菌群失调引起的腹泻症状有明显的治疗效果。且双歧杆菌、乳酸杆菌、屎肠球菌和酿酒酵母菌四种菌具有很好的耐酸耐胆盐效果,其组合物能有效改善肠道菌群结构、抑制病原菌、增加肠道有益菌群,提高机体免疫力,以达到防病、治病的效果。(The invention discloses a tetragenous viable bacteria preparation for treating diarrhea and application thereof, wherein the tetragenous viable bacteria preparation comprises 5-50% of probiotic mixture, 6-10% of xylooligosaccharide and the balance of glucose, and the probiotic mixture comprises animal bifidobacterium freeze-dried powder, lactobacillus paracasei freeze-dried powder, enterococcus faecium freeze-dried powder and saccharomyces cerevisiae freeze-dried powder. The composite live bacterium preparation of bifidobacterium, lactobacillus, enterococcus faecium and saccharomyces cerevisiae is used for treating diarrhea caused by dog intestinal flora imbalance, and has an obvious treatment effect on diarrhea symptoms caused by dog intestinal flora imbalance. The composition can effectively improve the intestinal flora structure, inhibit pathogenic bacteria, increase the intestinal beneficial flora and improve the immunity of the organism so as to achieve the effects of preventing and treating diseases.)

1. A tetrad viable bacteria preparation for treating diarrhea is characterized in that: comprises 5-50% of probiotic mixture, 6-10% of xylo-oligosaccharide and the balance of glucose;

the probiotic mixture comprises animal bifidobacterium freeze-dried powder, lactobacillus paracasei freeze-dried powder, enterococcus faecium freeze-dried powder and saccharomyces cerevisiae freeze-dried powder.

2. The live tetranection preparation for treating diarrhea according to claim 1, wherein: the viable count ratio of the bifidobacterium animalis, the lactobacillus paracasei, the enterococcus faecium and the saccharomyces cerevisiae is 10-20:10-20:1-2: 1-2.

3. The live tetranection preparation for treating diarrhea according to claim 1, wherein: the viable count of the bifidobacterium animalis B1014 strain is more than or equal to 2.0 multiplied by 10⁹CFU/g, the viable count of the lactobacillus paracasei L1056 strain is more than or equal to 2.0 multiplied by 10⁹CFU/g, the viable count of the enterococcus faecium EF1012 strain is more than or equal to 2.0 multiplied by 10⁸CFU/g, the number of viable bacteria of the saccharomyces cerevisiae S1072 strain is more than or equal to 2.0 multiplied by 10⁸CFU/g。

4. The live tetranection preparation for treating diarrhea according to claim 1, wherein: the survival rate of the strains is higher than 70 percent when the lactobacillus paracasei, the enterococcus faecium and the saccharomyces cerevisiae are cultured in gastric acid with the pH value of 3.0 for 4 hours; the survival rate of the bifidobacterium animalis strain cultured in gastric acid with the pH value of 3.0 for 4 hours is 58.3 percent, the survival rate of the bifidobacterium animalis strain cultured in 0.3 percent of No. 3 bile salt for 4 hours is higher than 50 percent, and the survival rate of the lactobacillus paracasei strain cultured in 0.3 percent of No. 3 bile salt for 2 hours is higher than 30 percent.

5. Use of a live tetra-bacterial formulation according to claim 1 for the treatment of diarrhoea, wherein: the live tetranection preparation is used for treating diarrhea caused by dog intestinal flora imbalance.

Technical Field

The invention relates to the technical field of medicines, in particular to a tetrad viable bacteria preparation for treating diarrhea and application thereof.

Background

The flora balance of the animal intestinal tract has an important influence on the health status of the digestive tract. In the pet market, represented by canines, antibiotics are the predominant drug. Although the development of various pathogenic bacteria such as campylobacter jejuni, escherichia coli, salmonella and other pathogenic bacteria and the symptoms of intestinal flora disturbance caused by some viral diseases are well controlled for a period of time, the symptoms take effect quickly, the defects after the antibiotics are used are gradually shown, such as the generation of drug-resistant pathogenic bacteria and the residue of drugs in livestock products, the antibiotics can also eliminate sensitive beneficial bacteria with the effects of protecting barriers and resisting external attack bacteria, the dysbacteriosis is aggravated, and then a series of typical symptoms of the intestinal flora disturbance such as vomiting, diarrhea, inappetence and the like are generated, and the fungal infection is caused to aggravate the diarrhea. Therefore, the diarrhea-affected dogs need to be treated by the probiotics, and the diarrhea-affected dogs need not to be treated blindly by antibiotics in clinical practice so as to prevent the further aggravation of the disturbance of the flora. And diseases caused by exogenous causes such as iatrogenic and food-borne diseases can be regulated and recovered by the probiotic. With the knowledge of serious disadvantages of antibiotics, probiotics gradually replace antibiotics, have various advantages of no side effect, capability of promoting animal growth and the like, and are widely concerned by people. Meanwhile, the intestinal flora imbalance of the dogs can be caused by exogenous factors such as too fast and too frequent change of diet, improper nutritional structure, environmental stress and the like.

However, the microecological preparation for dogs in the domestic market is irregular at present, and no microecological preparation for dogs is available.

Disclosure of Invention

The invention aims to provide a tetragenous viable bacteria preparation for treating diarrhea and application thereof.

In order to achieve the purpose, the invention provides the following technical scheme: a tetragenous viable bacteria preparation for treating diarrhea comprises 5-50% of probiotic mixture, 6-10% of xylooligosaccharide, and the balance of glucose;

the probiotic mixture comprises animal bifidobacterium freeze-dried powder, lactobacillus paracasei freeze-dried powder, enterococcus faecium freeze-dried powder and saccharomyces cerevisiae freeze-dried powder.

Furthermore, the viable count ratio of the animal bifidobacterium, the lactobacillus paracasei, the enterococcus faecium and the saccharomyces cerevisiae is 10-20:10-20:1-2: 1-2.

Furthermore, the viable count of the bifidobacterium animalis B1014 strain is more than or equal to 2.0 multiplied by 10⁹CFU/g, the viable count of the lactobacillus paracasei L1056 strain is more than or equal to 2.0 multiplied by 10⁹CFU/g, the viable count of the enterococcus faecium EF1012 strain is more than or equal to 2.0 multiplied by 10⁸CFU/g, the number of viable bacteria of the saccharomyces cerevisiae S1072 strain is more than or equal to 2.0 multiplied by 10⁸CFU/g。

Further, the survival rate of the strains is higher than 70 percent when the lactobacillus paracasei, the enterococcus faecium and the saccharomyces cerevisiae are cultured in gastric acid with the pH value of 3.0 for 4 hours; the survival rate of the bifidobacterium animalis strain cultured in gastric acid with the pH value of 3.0 for 4 hours is 58.3 percent, the survival rate of the bifidobacterium animalis strain cultured in 0.3 percent of No. 3 bile salt for 4 hours is higher than 50 percent, and the survival rate of the lactobacillus paracasei strain cultured in 0.3 percent of No. 3 bile salt for 2 hours is higher than 30 percent.

The invention also discloses an application of the tetrad viable bacteria preparation for treating diarrhea, which is characterized in that: the live tetranection preparation is used for treating diarrhea caused by dog intestinal flora imbalance.

Compared with the prior art, the invention has the beneficial effects that: the composite live bacterium preparation of bifidobacterium, lactobacillus, enterococcus faecium and saccharomyces cerevisiae is used for treating diarrhea caused by dog intestinal flora imbalance, the preparation has an obvious treatment effect on diarrhea symptoms caused by dog intestinal flora imbalance, and the bifidobacterium, the lactobacillus, the enterococcus faecium and the saccharomyces cerevisiae can effectively improve the intestinal flora structure, inhibit pathogenic bacteria and improve the immunity of organisms so as to achieve the effects of preventing and treating diseases.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.