阅读说明：本技术 一种抗蜜蜂麻痹病毒提取物及其制备方法 (Anti-bee paralysis virus extract and preparation method thereof ) 是由 侯春生 邓帅 杨卅 刁青云 王新玲 于 2019-10-12 设计创作，主要内容包括：本发明公开了一种抗蜜蜂麻痹病毒的组合物及其制剂与应用。该抗蜜蜂麻痹病毒的组合物,其特征在于,它由下述质量百分含量的组分组成：丹桂50-65％、八月桂25-35％,人参花5-25％。本发明的抗蜜蜂麻痹病毒的组合物适用于预防和治疗蜜蜂麻痹病及改善蜂群整体抗病毒能力。(The invention discloses a composition for resisting bee paralytic virus, and a preparation and application thereof. The composition for resisting the bee paralytic virus is characterized by comprising the following components in percentage by mass: 50-65% of cinnamon, 25-35% of Balaurel and 5-25% of ginseng flower. The composition for resisting the bee paralysis virus is suitable for preventing and treating the bee paralysis and improving the overall antiviral ability of bee colonies.)

1. The composition for resisting the bee paralytic virus is characterized by comprising the following components in percentage by mass: 50-65% of salvia miltiorrhiza and cassia bark leaf, 25-35% of august leaf and 5-25% of ginseng flower.

2. The composition against poliovirus of honey bees according to claim 1, wherein: the traditional Chinese medicine composition comprises 55% of salvia miltiorrhiza and cassia twig leaves, 30% of augusticum acutiloba leaves and 15% of ginseng flowers by mass percentage.

3. A preparation method of an anti-bee paralytic virus extract, which is prepared by the composition of claim 1 or 2, and comprises the following steps: extracting saponin from the ginseng flower, and then mixing the saponin with the salvia miltiorrhiza and cinnamomum japonicum leaf powder to obtain the bee paralysis virus resistant extract.

4. The method for preparing the anti-paralytic virus bee extract according to claim 3, comprising the steps of:

1) leaching flos Ginseng with 60-75% ethanol solution as solvent, collecting the solution, recovering ethanol, and concentrating at 55 deg.C to obtain saponin extract with relative density of 1.25-1.50;

2) pulverizing cortex Cinnamomi and Augusticica leaves by 80-120 mesh, and sieving;

3) adding the powder of the cinnamomum wilsonii leaves and the cinnamomum wilsonii leaves in the step 2) and the saponin extracting solution obtained in the step 1) into water, uniformly mixing, filtering to remove impurities, and concentrating the filtrate at 55 ℃ to obtain a concentrated solution with the relative density of 1.25-1.50, namely the bee paralysis virus resisting extract.

5. An anti-poliovirus extract prepared by the method of claim 3 or 4.

6. Use of the composition against poliovirus of claim 1 or 2 for the prevention and treatment of bee paralysis.

7. Use of the composition against poliovirus of honey bees according to claim 1 or 2 for improving the overall resistance of the bee colony against poliovirus.

8. The composition against poliovirus of claim 1 or 2, the use of the extract against poliovirus of claim 5 for preparing a preparation against poliovirus.

9. The use of the anti-poliovirus extract of claim 5 for the prevention and treatment of bee paralysis.

10. The use of the anti-paralytic virus bee extract of claim 5 to improve the overall resistance of a colony to paralytic viruses in bees.

Technical Field

The invention relates to an anti-bee paralysis virus extract and a preparation method thereof.

Background

Bees are social insects, and like other livestock and poultry animals, bees are also attacked by various diseases such as bacteria, fungi, viruses, parasites and the like, and among the various diseases of the bees, bee virus diseases are the most special and the most complex disease causing mechanisms. In addition, the globalization of bees and bee product trade leads to the rapid spread of bee diseases and enemies in different areas of the world, and seriously threatens the health of the bees. The virus disease is the most serious disease which is prevalent so far, and is also one of the important factors threatening the health of the bee colony. Although some chemicals are available, the efficacy is not significant and the virus is not completely eliminated, resulting in a latent infection of the virus in the bee colony and a new pandemic in the spring of the next year.

The mechanism for preventing and treating the bee paralytic virus globally available at present is mainly aimed at inhibiting or blocking RNA polymerase or helicase dependent on virus RNA. The rapid propagation of the virus can be reduced at that time, but the virus has the biggest characteristic of extremely rapid variation, and is easy to escape from host or drug resistance so as to change the sequence of the original gene, so that the virus disease cannot be effectively prevented and treated, and the risk of polluting bee products exists.

The natural product is considered to be the most widely used raw material for drug development or disease prevention and control all the time, and has the advantages of no toxicity, no harm, no toxic or side effect, easy degradation, no residue and capability of exerting the function of natural product functional molecules to the maximum extent.

The bee virus disease, especially one of the diseases with the biggest damage of paralysis disease to bees, is not solved completely by the insect virus field, once bees are infected, the bees are easy to remain latent, and great infectious sources and damage are caused.

Traditionally, viral diseases have not been treated as truly as other bacterial and parasitic diseases. However, with the rapid development of molecular biotechnology in this field, new substances with antiviral properties are continuously discovered. The existing medicine has the defects of large toxic and side effects, long-term use, inaccurate compatibility, temporary solution, virus eradication, failure in inhibiting the diffusion of the paralytic virus of bees, low immunity of bee colonies and finally death of the bee colonies.

Disclosure of Invention

The invention is based on modern biotechnology, and long-term experimental verification, is prepared by adopting a new process, and proves that the plant extract has great potential in treating the intractable bee disease caused by virus infection such as bee acute paralysis, israel bee paralysis, bee chronic paralysis and the like by preventing and treating a large number of bee colonies caused by bee paralysis virus.

Based on the above, the invention aims to provide the anti-bee paralysis virus composition which has proper drug compatibility, maintains the original natural component effects through a modern extraction process, has the effects of resisting virus, enhancing immunity, improving bee colony health and the like, is suitable for preventing and treating israeli bee paralysis, bee acute paralysis and bee chronic paralysis, as well as an extracting solution and a preparation thereof, and a preparation method thereof.

The composition for resisting the bee paralytic virus is composed of the following components in percentage by mass: 50-65% of salvia miltiorrhiza and cassia bark leaf, 25-35% of august leaf and 5-25% of ginseng flower.

As a preferable scheme of the invention, the composition for resisting the bee paralytic virus comprises 55% of cortex cinnamomi, 30% of fructus cinnamomi, and 15% of ginseng flower by mass.

The invention also provides an anti-bee paralysis virus extract.

The anti-bee paralytic virus extract is prepared by utilizing the composition, and the preparation method of the anti-bee paralytic virus extract comprises the following steps: extracting saponin from the ginseng flower, and then mixing the saponin with the salvia miltiorrhiza and cinnamomum japonicum leaf powder to obtain the bee paralysis virus resistant extract. Wherein, the method for extracting saponin by using ginseng flower is to extract by using 60-75% ethanol solution as solvent. The powder of the red sage root and cassia twig is obtained by superfine grinding, and the particle size is preferably 80-120 meshes.

In one embodiment of the present invention, the preparation method of the anti-bee paralytic virus extract comprises the following steps:

1) leaching flos Ginseng with 60-75 vol% ethanol solution as solvent, collecting the percolate, recovering ethanol, and concentrating at 55 deg.C to obtain flos Ginseng extractive solution with relative density of 1.25-1.50; the main component of the ginseng flower extract is saponin.

2) Pulverizing cortex Cinnamomi and Augusticica leaves by 80-120 mesh, and sieving;

3) adding the powder of the salvia miltiorrhiza and cinnamomum bayenii leaves in the step 2) and the saponin extracting solution obtained in the step 1) into water, uniformly mixing, filtering (80-120 meshes), and concentrating the filtrate at 55 ℃ to obtain a concentrated solution with the relative density of 1.25-1.50, namely the bee paralysis virus resisting extract.

The above extract can also be made into capsule, paste or spray.

The application of the composition for resisting the bee paralysis virus or the extract for resisting the bee paralysis virus in preventing and treating the bee paralysis disease and the application in improving the integral resistance of bee colonies to the bee paralysis virus also belong to the protection scope of the invention.

The composition for resisting the bee paralysis virus has proper compatibility of medicines, maintains the original natural component effects through a modern extraction process, has the effects of resisting viruses, enhancing immunity, improving bee colony health and the like, is suitable for resisting the bee paralysis virus for preventing and treating the israeli bee paralysis, the bee acute paralysis and the bee chronic paralysis, and has excellent effect.

Drawings

FIG. 1 shows the antiviral effect of the composition against poliovirus of example 3.

Fig. 2 is a comparison of the test results of dead bees on the fifth and sixth days with the blank control group. Lanes 5 and 6 are blank control bee samples on fifth and sixth days, respectively; lanes 1, 7 are day five and six virus-infected cohort bee-like samples; lanes 4, 8 are day six virus-infected cohort bee-like samples; lanes 2 and 3 are control bee samples at day five and six virus plus antiviral extract doses of 100 μ g/mL; lanes 9 and 10 are bee samples from day five and six virus groups at a dose of 10 μ g/mL antiviral extract; lane 11 is a fifth day virus plus antiviral extract dose of 50. mu.g/mL swarms.

Detailed Description

The methods in the following examples are conventional methods unless otherwise specified.

The materials used in the examples of the invention are as follows:

leaf of red-rooted cinnamon: purchased from online mall of Alibara, weights are on a dry basis.

And (3) Bayue cinnamon leaf: purchased from online mall of Alibara, weights are on a dry basis.

Ginseng flower: purchased from online mall of Alibara, weights are on a dry basis.