阅读说明：本技术 一种dna提取方法 (DNA extraction method ) 是由 王中刚 程郎 汪玉洁 于 2019-09-04 设计创作，主要内容包括：本发明属于遗传工程技术领域,具体的说是一种DNA提取方法,包括包括上壳体、下壳体、缓冲液储存室、底板、滑动塞、传动轴和碾压轮；所述缓冲液储存室固接在上壳体顶部；所述底板固接在缓冲液储存室内侧底部；所述传动轴贯穿上壳体一侧侧壁中部,并安装到相对侧壁中部,传动轴与侧壁间通过轴承连接；所述传动轴中部间隔套接有碾压轮,碾压轮偏心固接在传动轴中部,且传动轴中心线距离碾压轮中心线距离均相等,两个碾压轮之间相互旋转对称；本发明通过设置上壳体和下壳体的组装方式,使得仪器使用起来更加方便,而且使得DNA提取仪器更加简单,方便携带,能够满足室外各种天气环境下对样本DNA的提取。(The invention belongs to the technical field of genetic engineering, and particularly relates to a DNA extraction method, which comprises an upper shell, a lower shell, a buffer solution storage chamber, a bottom plate, a sliding plug, a transmission shaft and a rolling wheel; the buffer solution storage chamber is fixedly connected to the top of the upper shell; the bottom plate is fixedly connected to the bottom of the inner side of the buffer solution storage chamber; the transmission shaft penetrates through the middle part of the side wall of one side of the upper shell and is installed in the middle part of the opposite side wall, and the transmission shaft is connected with the side wall through a bearing; the middle part of the transmission shaft is sleeved with rolling wheels at intervals, the rolling wheels are eccentrically and fixedly connected to the middle part of the transmission shaft, the distances from the center line of the transmission shaft to the center lines of the rolling wheels are equal, and the two rolling wheels are rotationally symmetrical; according to the invention, the upper shell and the lower shell are assembled, so that the instrument is more convenient to use, and the DNA extraction instrument is simpler and more convenient to carry, and can be used for extracting the sample DNA in outdoor various weather environments.)

1. A DNA extraction method is characterized by comprising the following steps:

S1, selecting a sample, selecting the tender part as much as possible, facilitating the grinding and crushing to extract the DNA, cleaning the sample with distilled water, and removing the rotten and abnormal part in the sample to avoid the interference of the rotten part of the microorganism on the extraction of the DNA;

s2, putting the sample in S1 into a DNA extraction instrument, grinding and crushing the sample, adding an extracting solution, and destroying cell walls of cells to expose DNA parts so as to extract the DNA parts;

S3, after the sample in S2 is rolled and crushed, adding a buffer solution into the sample, and continuing to roll or stir the sample to fully mix the buffer solution with the sample so as to gradually extract DNA in the cells;

S4, after the sample in S3 fully acts with the buffer solution, filtering the mixed solution after the sample is rolled, standing the mixed solution to obtain the supernatant of the mixed solution, wherein the supernatant contains the DNA of the sample, performing an experiment on the DNA in the supernatant, recording the concrete condition of the DNA, and finally storing and keeping the supernatant;

the DNA extraction instrument used in the S2 and S3 comprises an upper shell (1), a lower shell (2), a buffer solution storage chamber (3), a bottom plate (31), water passing holes (32), a plug (33), arc-shaped holes (35), a transmission shaft (4), a coupler (41), a rolling wheel (5), a rolling net (6), a support belt (7), tapered holes (71) and an extracting solution storage chamber (8); the buffer solution storage chamber (3) is fixedly connected to the top of the upper shell (1); the bottom plate (31) is fixedly connected to the bottom of the inner side of the buffer solution storage chamber (3); an arc-shaped hole (35) is formed in the middle of the bottom plate (31), and one end, close to the tip end of the bottom plate (31), of the arc-shaped hole (35) is communicated with the inside of the upper shell (1); a water through hole (32) is formed in the middle of the top of the bottom plate (31), and the water through hole (32) is communicated with the arc-shaped hole (35); a horizontal hole is formed in the tip end of the bottom plate (31), the horizontal hole is intersected with the arc-shaped hole (35), and the aperture of the horizontal hole is larger than that of the arc-shaped hole (35); a plug (33) is arranged in the horizontal hole; one end of the transmission shaft (4) penetrates through the middle part of the side wall of one side of the upper shell (1) and is installed in the middle part of the opposite side wall, the transmission shaft (4) is connected with the side wall through a bearing, and the other end of the transmission shaft (4) is located outside the upper shell (1) and is provided with a coupler (41); the middle part of the transmission shaft (4) is sleeved with rolling wheels (5) at intervals, the rolling wheels (5) are eccentrically and fixedly connected to the middle part of the transmission shaft (4), the distances from the central line of the transmission shaft (4) to the central line of the rolling wheels (5) are equal, and the two rolling wheels (5) are rotationally symmetrical; a supporting rod is arranged at the position, closest to the transmission shaft (4), of the side surface adjacent to the rolling wheel (5), a bearing hole is formed in the position, opposite to the supporting rod, of the side surface, and the supporting rod on one side of the rolling wheel (5) is connected into the bearing hole in the side surface adjacent to the other side of the rolling wheel (5); the rolling net (6) is fixedly connected to the bottom side of the inside of the upper shell (1), the rolling net (6) is of a concave structure, and when the wheel edge of the rolling wheel (5) which is farthest away from the transmission shaft (4) is located below, the bottom of the rolling net (6) is extruded by the rolling wheel (5); the top end of the lower shell (2) is provided with a thread groove, the bottom of the upper shell (1) is provided with threads, and the top end of the lower shell (2) is fixedly connected to the bottom end of the upper shell (1) through thread engagement; a supporting belt (7) is arranged at the top end inside the lower shell (2); support area (7) middle part and be provided with bell mouth (71), support area (7) below is extract apotheca (8).

2. The method for extracting DNA according to claim 1, wherein: arc-shaped leakage grooves (51) are formed in two sides of the rolling wheel (5), and the arc-shaped leakage grooves (51) are outwards diffused by taking the transmission shaft (4) as a diffusion center.

3. the method for extracting DNA according to claim 1, wherein: a piston plate (81) is arranged at the bottom of the inner side of the lower shell (2); an air vent (82) is formed in one side of the bottom of the lower shell (2), and an air cylinder (83) is arranged on the outer side of the lower shell (2); an air pressure piston (84) is arranged in the air cylinder (83); the top of the air pressure piston (84) is fixedly connected with a push rod (85); the push rod (85) penetrates through the top of the air cylinder (83) and is connected to the bottom side of the handle (86), and the connection part of the push rod (85) and the air cylinder (83) is connected through threads.

4. The method for extracting DNA according to claim 1, wherein: a stop lever (34) is arranged in the arc-shaped hole (35); a fracture is arranged in the middle of the stop lever (34), a spring is connected to the fracture, and two ends of the spring are fixedly connected to two fracture surfaces of the fracture in the middle of the stop lever (34) respectively; a water passing groove is arranged on the outer side of one end, close to the plug (33), of the stop lever (34), the water passing groove is in a thread shape, and the other end of the water passing groove corresponds to the position of the water passing hole (32); sliding plugs (341) are arranged at two ends of the stop lever (34), and the sliding plugs (341) are made of magnet materials; magnet blocks (52) are arranged on the wheel edges of the positions, farthest from the transmission rod, of the rolling wheels (5), and the two magnet blocks (52) on the wheel edges of different rolling wheels (5) correspond to the positions of the magnet material sliding plugs (341) at the two ends of the stop rod (34) respectively and attract each other magnetically.

5. the method for extracting DNA according to claim 4, wherein: the cross section of the sliding plug (341) is a regular hexagon, the cross section of the arc-shaped hole (35) where the sliding plug (341) is located is a regular hexagon, the arc-shaped hole (35) is of a prismatic table structure, the cross section area of the regular hexagon at the topmost end of the prismatic table is smaller than that of the sliding plug (341), and the cross section area of the regular hexagon at the bottommost end of the prismatic table is larger than that of the sliding plug (341).

6. the method for extracting DNA according to claim 1, wherein: the included angle between the bottom surface and the side surface of the rolling net (6) is smaller than the included angle between the bottom surface of the supporting belt (7) and the side surface of the supporting belt (7).

Technical Field

The invention belongs to the technical field of genetic engineering, and particularly relates to a DNA extraction method.

background

Along with the development of the scientific and technical level, the biological gene technology is also a new and advanced technology, when some rare species or variant species are researched, NDA extraction and analysis are often performed on the rare species or variant species, so that the genetic condition of the rare species or variant species is known, the biological characteristics of the rare species or variant species are researched, so that the root cause of the unique external expression characteristics of the organisms can be known, DNA extraction is gradually and frequently performed in the process, in addition, many scientific research teams and exploration teams also need to perform DNA extraction on some discovered biological samples in the field, as is well known, scientific research is performed in the field, and more things need to be carried, so that the used DNA extraction instrument needs to meet the characteristics of portability, reliability and practicability, and can meet the use requirements under various weather conditions in the field.

The existing DNA extraction instrument is heavy and inconvenient to carry manually, various different experimental utensils are required to be used in the using process, the carrying is more unfavorable, and the DNA extraction instrument is easy to damage, so that the DNA extraction instrument which is convenient to carry and use is more and more important to biological research and scientific research processes.

Disclosure of Invention

In order to make up for the defects of the prior art and solve the problems, the DNA extraction method provided by the invention has the advantages that the DNA extraction instrument is more convenient to use by arranging the upper shell and the lower shell in an assembling mode, the DNA extraction instrument is simpler and is convenient to carry, and the extraction of sample DNA in various outdoor weather environments can be met.

The technical scheme adopted by the invention for solving the technical problems is as follows: the DNA extraction method comprises the following steps:

s1, selecting a sample, selecting the tender part as much as possible, facilitating the grinding and crushing to extract the DNA, cleaning the sample with distilled water, and removing the rotten and abnormal part in the sample to avoid the interference of the rotten part of the microorganism on the extraction of the DNA;

s2, putting the sample in S1 into a DNA extraction instrument, grinding and crushing the sample, adding an extracting solution, and destroying cell walls of cells to expose DNA parts so as to extract the DNA parts;

S3, after the sample in S2 is rolled and crushed, adding a buffer solution into the sample, and continuing to roll or stir the sample to fully mix the buffer solution with the sample so as to gradually extract DNA in the cells;

S4, after the sample in S3 fully acts with the buffer solution, filtering the mixed solution after the sample is rolled, standing the mixed solution to obtain the supernatant of the mixed solution, wherein the supernatant contains the DNA of the sample, performing an experiment on the DNA in the supernatant, recording the concrete condition of the DNA, and finally storing and keeping the supernatant;

The DNA extraction instrument used in the S2 and S3 comprises an upper shell, a lower shell, a buffer solution storage chamber, a bottom plate, a water passing hole, a plug, an arc-shaped hole, a transmission shaft, a coupler, a rolling wheel, a rolling net, a supporting belt, a conical hole and an extracting solution storage chamber; the buffer solution storage chamber is fixedly connected to the top of the upper shell; the bottom plate is fixedly connected to the bottom of the inner side of the buffer solution storage chamber; an arc-shaped hole is formed in the middle of the bottom plate, and one end, close to the tip end of the bottom plate, of the arc-shaped hole is communicated with the interior of the upper shell; a water passing hole is formed in the middle of the top of the bottom plate and is communicated with the arc-shaped hole; the top end part of the bottom plate is provided with a horizontal hole, the horizontal hole is intersected with the arc-shaped hole, and the aperture of the horizontal hole is larger than that of the arc-shaped hole; a plug is arranged in the horizontal hole; the transmission shaft penetrates through the middle part of the side wall of one side of the upper shell and is installed in the middle part of the opposite side wall, the transmission shaft is connected with the side wall through a bearing, and the other end of the transmission shaft is positioned outside the upper shell and is provided with a coupler; the middle part of the transmission shaft is sleeved with rolling wheels at intervals, the rolling wheels are eccentrically and fixedly connected to the middle part of the transmission shaft, the distances from the center line of the transmission shaft to the center lines of the rolling wheels are equal, and the two rolling wheels are rotationally symmetrical; a supporting rod is arranged at the position, closest to the transmission shaft, of the adjacent side surface of the rolling wheel, a bearing hole is formed in the position, opposite to the supporting rod, of the side surface, and the supporting rod on one side of the rolling wheel is connected into the bearing hole in the adjacent side surface of the other side of the rolling wheel; the rolling net is fixedly connected to the bottom side in the upper shell, the rolling net is of a concave structure, and when the wheel edge of the rolling wheel, which is farthest away from the transmission shaft, is positioned below the transmission shaft, the bottom of the rolling net is extruded by the rolling wheel; the top end of the lower shell is provided with a thread groove, the bottom of the upper shell is provided with threads, and the top end of the lower shell is fixedly connected to the bottom end of the upper shell through thread engagement; a supporting belt is arranged at the top end of the inner part of the lower shell; the support belt middle part is provided with the bell mouth, the support belt below is the extract apotheca. When the device works, the current DNA extraction instrument is heavy and inconvenient to carry, so that in outdoor environment, the extraction of DNA samples is complex, especially in the field environment of various geographical and weather conditions, the DNA of fresh samples is difficult to extract in time, the device is greatly simplified on the premise of ensuring the smooth extraction of the DNA of the samples, firstly, a buffer solution (generally Tris-cl solution with the concentration of 0.1M, pH8.0) is placed in a buffer solution storage chamber at the top, a plug is plugged, an extracting solution (generally NAOH solution with the concentration of 0.4) is added into the extracting solution storage chamber, the samples are placed between a rolling net and a supporting belt, after an upper shell and a lower shell are erected, a coupler at the outer end of a transmission shaft is connected to a motor shaft of the motor, the motor is electrified, the motor rotates, the rolling wheel is driven to rotate by the transmission shaft, and the rolling wheel is eccentrically arranged on the transmission shaft, after the rolling wheel rotates, the rolling wheels on two sides can sequentially extrude the rolling net below, so that the rolling net deforms, and the sample below is rolled and crushed, under the pressure action of the rolling net, the supporting belt also deforms, in the process of downward deformation of the supporting belt, the conical hole in the middle of the supporting belt also deforms, the conical opening below is expanded after the conical opening deforms, so that along with the rolling, the conical hole is also opened, the extracting solution below overflows, the extracting solution cannot be continuously added after the water level of the extracting solution drops to a certain degree, under the action of the extracting solution, the cell wall is gradually crushed, DNA is gradually exposed, after the rolling is completed, the plug of the buffer solution storage chamber is opened, the buffer solution flows into the upper shell through the arc-shaped hole and is sprinkled on the sample, at the moment, the rolling wheels continue to rotate, accelerating the effect of the buffer solution on the sample, accelerating the DNA extraction efficiency, plugging a plug of the buffer solution storage chamber after the extraction is finished, closing a motor power supply, inverting the device, filtering and sprinkling the rolled sample mixed solution downwards by the rolling net, and removing the supernatant to analyze the DNA of the sample after standing.

Preferably, the rolling wheel both sides set up the arc small opening groove, and the arc small opening groove all uses the transmission shaft to outwards disperse as the center of dispersing. The during operation, when buffer solution is to the trickle down, the inhomogeneous situation of trickle can appear unavoidably to lead to the inhomogeneous situation of buffer solution effect to appear, and the arc small opening can be broken up the buffer solution that spills down and even trickle on the sample, makes buffer solution and sample fully contact, and even effect is favorable to improving DNA extraction efficiency on all samples.

preferably, a piston plate is arranged at the bottom of the inner side of the lower shell; an air vent is formed in one side of the bottom of the lower shell, and an air cylinder is arranged outside the lower shell; the air cylinder is internally provided with an air pressure piston; the top of the air pressure piston is fixedly connected with a push rod; the push rod penetrates through the top of the inflator and is connected to the bottom side of the handle, and the connection position of the push rod and the inflator is in threaded connection. During operation, the extracting solution inside the lower shell overflows upwards in the rolling process, the extracting solution is stopped being provided after the water level of the extracting solution drops, therefore, the dosage of the supplying of the extracting solution needs to be controlled under some special conditions, the water level of the extracting solution needs to be controlled, when the water level of the extracting solution is too high, the handle can be rotated upwards, the volume in the air cylinder is increased, the air at the bottom of the lower shell is sucked into the air cylinder, in order to maintain the air pressure balance, the piston plate below the lower shell drops, the water level of the extracting solution further drops, when the water level of the extracting solution is too low, the handle is rotated downwards, the air in the air cylinder is squeezed into the lower shell, the piston plate can be jacked, and the.

Preferably, a stop lever is arranged in the arc-shaped hole; a fracture is arranged in the middle of the stop lever, a spring is connected to the fracture, and two ends of the spring are fixedly connected to two fracture surfaces of the fracture in the middle of the stop lever respectively; the outer side of one end, close to the plug, of the stop lever is provided with a water passing groove, the water passing groove is in a thread shape, and the other end of the water passing groove corresponds to the position of the water passing hole; sliding plugs are arranged at two ends of the stop lever and made of magnet materials; the wheel edge of the farthest position of the rolling wheel from the transmission rod is provided with a magnet block, and the two magnet blocks on the wheel edges of different rolling wheels correspond to the positions of the magnet material sliding plugs at the two ends of the stop rod respectively and attract each other magnetically. During operation, the interpolation of buffer solution needs progressively a small amount of shower on the sample surface, consequently, need carry out the management and control to the shower of buffer solution, just can make the rolling wheel roll the sample in-process and progressively shower buffer solution, under normal conditions, the pin is under the tensile force effect of middle part spring, the slip stopper at both ends can tightly laminate at the water hole both ends, prevent the buffer solution to pass through, when the rolling wheel is located the top apart from the wheel limit of transmission shaft farthest, the magnet piece of this department just can attract the slip stopper, another rolling wheel is located the below apart from the wheel limit of transmission shaft farthest this moment, it is far away from the pin position, consequently, under the attraction effect, the pin takes place to move in the arc hole, the slip stopper that is close to the rolling wheel is attracted by the magnet piece of rolling wheel limit, the spring at pin middle part is elongated, the slip stopper leaves this department arc hole, the jam of arc hole, make the buffer solution shower from the top, if the stopper is in the open mode this moment, buffer solution alright in order to accomplish the shower to the sample, because the rolling wheel sets up for rotational symmetry, consequently along with the rotation of transmission shaft, the magnet piece of the farthest department of transmission shaft will be located the top position in turn separately, make pin both ends slip stopper receive the appeal effect in turn, consequently the pin just can be at the downthehole round trip movement of arc, and then make the buffer solution opening open and shut along with the rotation of rolling wheel, buffer solution also can progressively shower downwards, the outer water trough of crossing of pin can help buffer solution flow direction opening part.

preferably, the sliding plug cross section is regular hexagon, and the arc hole cross section at sliding plug place is regular hexagon, and this department arc hole is the terrace with edge structure, and terrace with edge regular hexagon cross sectional area at the top is littleer than sliding plug regular hexagon cross sectional area, and terrace with edge regular hexagon cross sectional area at the bottom is bigger than sliding plug regular hexagon cross sectional area. The during operation, the slip stopper can be along with the rotation of rolling wheel and at the downthehole round trip movement of arc, block in proper order and open the opening in arc hole, regular hexagon's cross sectional shape can make the slip stopper be the plane contact with the contact surface in this arc hole, resistance when having reduced the slip stopper and outwards having slided, make the slip stopper round trip movement more swift, and the mode of plane contact also makes the slip stopper more inseparable with the contact in this arc hole, be favorable to the slip stopper to the sealed of buffer solution opening part.

Preferably, the included angle between the bottom surface and the side surface of the rolling net is smaller than the included angle between the bottom surface and the side surface of the supporting belt. The during operation, the rolling wheel extrudees rolling net earlier, make rolling net carry out deformation, then rolling net extrudees the support area of below again, consequently, rolling net's deformation volume is big than the deformation volume of supporting the area, consequently at the in-process that rolls, if support the contained angle between area bottom surface and the support area side is less, so support the area and roll net and can form the clearance in this department, rolling net can't roll the sample of this department, thereby influence the extraction of DNA, consequently, rolling net bottom surface and the contained angle between the side is less than the contained angle that supports between area bottom surface and the support area side and can guarantee that the sample of controlling about can both be fine rolls the breakage.

The invention has the following beneficial effects:

1. according to the DNA extraction method, the upper shell and the lower shell are assembled, so that the DNA extraction instrument can be normally placed to perform operations such as grinding, crushing and the like on a sample, the instrument can be inverted to perform operations such as extraction, standing and the like, the DNA extraction instrument is more convenient to use, the DNA extraction instrument is simpler and more convenient to carry, and the extraction of the sample DNA in outdoor various weather environments can be met.

2. According to the DNA extraction method, the arc-shaped leakage groove is arranged, so that the sprayed buffer solution can be scattered and uniformly sprayed on the sample, the buffer solution is fully contacted with the sample and uniformly acts on all the samples, and the DNA extraction efficiency is improved.

drawings

The invention will be further explained with reference to the drawings.

FIG. 1 is a flow chart of the present invention;

FIG. 2 is a sectional view of the DNA extracting apparatus according to the present invention;

FIG. 3 is an enlarged view of a portion of FIG. 2 at A;

Figure 4 is a perspective view of the rolling wheel;

FIG. 5 is a perspective view of the end of the stop lever;

In the figure: the device comprises an upper shell 1, a lower shell 2, a buffer solution storage chamber 3, a bottom plate 31, a water through hole 32, a plug 33, a stop lever 34, a sliding plug 341, an arc hole 35, a transmission shaft 4, a coupler 41, a rolling wheel 5, an arc leakage groove 51, a magnet block 52, a rolling net 6, a supporting belt 7, a tapered hole 71, an extracting solution storage chamber 8, a piston plate 81, an air vent 82, an air cylinder 83, an air pressure piston 84, a push rod 85 and a handle 86.

Detailed Description

In order to make the technical means, the creation characteristics, the achievement purposes and the effects of the invention easy to understand, the invention is further described with the specific embodiments.

As shown in fig. 1 to 5, the DNA extraction method of the present invention includes the following steps:

S1, selecting a sample, selecting the tender part as much as possible, facilitating the grinding and crushing to extract the DNA, cleaning the sample with distilled water, and removing the rotten and abnormal part in the sample to avoid the interference of the rotten part of the microorganism on the extraction of the DNA;

S2, putting the sample in S1 into a DNA extraction instrument, grinding and crushing the sample, adding an extracting solution, and destroying cell walls of cells to expose DNA parts so as to extract the DNA parts;

S3, after the sample in S2 is rolled and crushed, adding a buffer solution into the sample, and continuing to roll or stir the sample to fully mix the buffer solution with the sample so as to gradually extract DNA in the cells;

S4, after the sample in S3 fully acts with the buffer solution, filtering the mixed solution after the sample is rolled, standing the mixed solution to obtain the supernatant of the mixed solution, wherein the supernatant contains the DNA of the sample, performing an experiment on the DNA in the supernatant, recording the concrete condition of the DNA, and finally storing and keeping the supernatant;

The DNA extraction instrument used in the S2 and S3 comprises an upper shell 1, a lower shell 2, a buffer solution storage chamber 3, a bottom plate 31, a water through hole 32, a plug 33, an arc-shaped hole 35, a transmission shaft 4, a coupler 41, a rolling wheel 5, a rolling net 6, a support belt 7, a tapered hole 71 and an extracting solution storage chamber 8; the buffer solution storage chamber 3 is fixedly connected to the top of the upper shell 1; the bottom plate 31 is fixedly connected to the bottom of the inner side of the buffer solution storage chamber 3; an arc-shaped hole 35 is formed in the middle of the bottom plate 31, and one end, close to the tip end of the bottom plate 31, of the arc-shaped hole 35 is communicated with the inside of the upper shell 1; a water through hole 32 is formed in the middle of the top of the bottom plate 31, and the water through hole 32 is communicated with the arc-shaped hole 35; a horizontal hole is formed in the tip end of the bottom plate 31 and is intersected with the arc-shaped hole 35, and the aperture of the horizontal hole is larger than that of the arc-shaped hole 35; a plug 33 is arranged in the horizontal hole; the transmission shaft 4 penetrates through the middle part of the side wall of one side of the upper shell 1 and is installed in the middle part of the opposite side wall, the transmission shaft 4 is connected with the side wall through a bearing, and the other end of the transmission shaft 4 is positioned outside the upper shell 1 and is provided with a coupler 41; the middle part of the transmission shaft 4 is sleeved with rolling wheels 5 at intervals, the rolling wheels 5 are eccentrically and fixedly connected to the middle part of the transmission shaft 4, the distances from the center line of the transmission shaft 4 to the center lines of the rolling wheels 5 are equal, and the two rolling wheels 5 are rotationally symmetrical; a supporting rod is arranged at the position, closest to the transmission shaft 4, of the side surface of the rolling wheel 5, a bearing hole is formed in the position, opposite to the supporting rod, of the side surface, and the supporting rod on the rolling wheel 5 on one side is connected into the bearing hole in the adjacent side surface of the rolling wheel 5 on the other side; the rolling net 6 is fixedly connected to the bottom side of the inner part of the upper shell 1, the rolling net 6 is of a concave structure, and when the wheel edge of the rolling wheel 5, which is farthest away from the transmission shaft 4, is positioned below, the bottom of the rolling net 6 is extruded by the rolling wheel 5; the top end of the lower shell 2 is provided with a thread groove, the bottom of the upper shell 1 is provided with threads, and the top end of the lower shell 2 is fixedly connected to the bottom end of the upper shell 1 through thread engagement; a supporting belt 7 is arranged at the top end inside the lower shell 2; the middle part of the supporting belt 7 is provided with a tapered hole 71, and an extracting solution storage chamber 8 is arranged below the supporting belt 7. When the DNA extraction instrument works, the existing DNA extraction instrument is heavy and inconvenient to carry, so that in an outdoor environment, the extraction of DNA samples is complex, particularly in a field environment with various geographical and weather conditions, the DNA of fresh samples is difficult to extract in time, the invention realizes great simplification of equipment on the premise of ensuring the smooth extraction of the DNA of the samples, firstly, a buffer solution (generally Tris-cl solution with the concentration of 0.1M, pH8.0) is placed in a buffer solution storage chamber 3 at the top, a plug 33 is plugged, an extracting solution (generally NAOH solution with the concentration of 0.4) is added into the extracting solution storage chamber 8, the samples are placed between a rolling net 6 and a supporting belt 7, after an upper shell 1 and a lower shell 2 are erected, a coupler 41 at the outer end of a transmission shaft 4 is connected to a motor shaft of a motor, the motor is electrified, the motor rotates, and then a rolling wheel 5 is driven by the transmission shaft 4 to rotate, the rolling wheel 5 is eccentrically arranged on the transmission shaft 4, after the rolling wheel 5 rotates, the rolling wheels 5 on two sides can sequentially extrude the rolling net 6 below, so that the rolling net 6 deforms, and the sample below is rolled and crushed, under the pressure action of the rolling net 6, the supporting belt 7 also deforms, in the process that the supporting belt 7 deforms downwards, the tapered hole 71 in the middle of the supporting belt 7 also deforms, the tapered hole below deforms and is then opened, so that the tapered hole 71 can be opened along with the rolling, the extracting solution below overflows, when the water level of the extracting solution drops to a certain degree, the extracting solution cannot be continuously added, under the action of the extracting solution, the cell wall is gradually crushed, DNA is gradually exposed, after the rolling is finished, the plug 33 of the buffer solution storage chamber 3 is opened, so that the buffer solution flows into the upper shell 1 through the arc-shaped hole 35 from the water through hole 32 and is sprayed on the sample, at the moment, the rolling wheel 5 continues to rotate to accelerate the action of the buffer solution on the sample and accelerate the DNA extraction efficiency, after the extraction is finished, a plug 33 of the buffer solution storage chamber 3 is plugged, the power supply of the motor is turned off, the equipment is inverted, the rolled sample mixed solution is downwards filtered and sprinkled by the rolling net 6, and after the standing is finished, the supernatant is removed to analyze the DNA of the sample.

in one embodiment of the present invention, the rolling wheels 5 are provided with arc-shaped leakage grooves 51 at both sides, and the arc-shaped leakage grooves 51 are all outwardly divergent with the transmission shaft 4 as a divergent center. The during operation, when buffer solution is to the trickle down, the inhomogeneous situation of trickle can appear unavoidably to lead to the inhomogeneous situation of buffer solution effect to appear, and arc small opening 51 can break up the buffer solution that spills down and even trickle on the sample, makes buffer solution and sample fully contact, and even effect is favorable to improving DNA extraction efficiency on all samples.

As an embodiment of the present invention, a piston plate 81 is provided at the bottom inside the lower housing 2; an air vent 82 is formed in one side of the bottom of the lower shell 2, and an air cylinder 83 is arranged on the outer side of the lower shell 2; an air pressure piston 84 is arranged in the air cylinder 83; the top of the air pressure piston 84 is fixedly connected with a push rod 85; the push rod 85 penetrates the top of the air cylinder 83 and is connected to the bottom side of the handle 86, and the connection part of the push rod 85 and the air cylinder 83 is connected through threads. In operation, the extracting solution inside the lower casing 2 overflows upwards in the rolling process, the extracting solution supply is stopped after the extracting solution level drops, therefore, the dosage of the extracting solution supply needs to be controlled under some special conditions, the extracting solution level needs to be controlled, when the extracting solution level is too high, the handle 86 can be rotated upwards, the volume in the air cylinder 83 is increased, the air at the bottom of the lower casing 2 is sucked into the air cylinder 83, the piston plate 81 below the lower casing 2 is lowered to further lower the extracting solution level, when the extracting solution level is too low, the handle 86 is rotated downwards, the air in the air cylinder 83 is squeezed into the lower casing 2 to jack the piston plate 81, and the extracting solution level is raised.

as an embodiment of the present invention, a stop lever 34 is installed inside the arc-shaped hole 35; a fracture is arranged in the middle of the stop lever 34, a spring is connected to the fracture, and two ends of the spring are fixedly connected to two fracture surfaces of the fracture in the middle of the stop lever 34 respectively; a water passing groove is arranged on the outer side of one end, close to the plug 33, of the stop lever 34, the water passing groove is in a thread shape, and the other end of the water passing groove corresponds to the position of the water passing hole 32; sliding plugs 341 are arranged at both ends of the stop lever 34, and the sliding plugs 341 are made of magnet material; the wheel edges of the farthest positions of the rolling wheels 5 from the transmission rod are provided with magnet blocks 52, and the two magnet blocks 52 on the wheel edges of different rolling wheels 5 correspond to the magnet material sliding plugs 341 at the two ends of the stop rod 34 respectively in position and are magnetically attracted. During operation, the interpolation of buffer solution needs progressively a small amount of shower on the sample surface, consequently, need carry out the management and control to the shower of buffer solution, just can make rolling wheel 5 roll the sample in-process progressively shower buffer solution, under normal conditions, pin 34 is under the pulling force effect of middle part spring, the sliding plug 341 at both ends can tightly laminate at water hole 32 both ends, prevent the buffer solution to pass through, when rolling wheel 5 is located the top apart from the wheel limit of transmission shaft 4 farthest, magnet piece 52 of this department just can attract sliding plug 341, another rolling wheel 5 is located the below apart from the wheel limit of transmission shaft 4 farthest this moment, distance pin 34 position is far away, consequently, under the attraction effect, pin 34 takes place to remove in arc hole 35, sliding plug 341 near rolling wheel 5 is attracted by the magnet piece 52 of rolling wheel 5 wheel limit, the spring in pin middle part is lengthened, sliding plug 341 leaves this arc hole 35, arc hole 35 loses the jam of sliding plug 341, make buffer solution shower from the top, if stopper 33 is in the open mode this moment, buffer solution alright accomplish the shower to the sample, because rolling wheel 5 sets up for rotational symmetry, consequently along with the rotation of transmission shaft 4, magnet piece 52 apart from 4 farthest departments of transmission shaft will be located the top position in turn separately, make stop lever 34 both ends sliding plug 341 receive the suction in turn, consequently stop lever 34 just can round trip movement in arc hole 35, and then make the buffer solution opening open and shut along with rolling wheel 5's rotation, buffer solution also can shower downwards gradually, the water trough of crossing of stop lever 34 outer layer can help buffer solution flow direction opening part.

as an embodiment of the present invention, the cross section of the sliding plug 341 is a regular hexagon, the cross section of the arc hole 35 where the sliding plug 341 is located is a regular hexagon, the arc hole 35 is in a frustum of a prism structure, the cross section area of the regular hexagon at the topmost end of the frustum of a prism is smaller than that of the regular hexagon of the sliding plug 341, and the cross section area of the regular hexagon at the bottommost end of the frustum of a prism is larger than that of the regular hexagon of the sliding plug 341. During operation, sliding plug 341 can be along with rolling wheel 5's rotation and round trip movement in arc hole 35, block in proper order and open the opening in arc hole 35, regular hexagon's cross sectional shape can make sliding plug 341 and the contact surface in this department arc hole 35 be the plane contact, resistance when sliding plug 341 outwards slides has been reduced, make sliding plug 341 round trip movement more swift, and the mode of plane contact also makes sliding plug 341 and the contact in this department arc hole 35 more inseparable, be favorable to sliding plug 341 to the sealed of buffer solution opening part.

in one embodiment of the present invention, the angle between the bottom surface and the side surface of the crushing net 6 is smaller than the angle between the bottom surface of the supporting belt 7 and the side surface of the supporting belt 7. The during operation, rolling wheel 5 extrudees rolling net 6 earlier, make rolling net 6 carry out deformation, then rolling net 6 extrudees supporting band 7 of below again, consequently, the deformation volume of rolling net 6 is bigger than the deformation volume of supporting band 7, consequently at the in-process that rolls, if supporting band 7 bottom surface and supporting band 7 contained angle between the side is less, supporting band 7 and rolling net 6 can form the clearance in this department so, rolling net 6 can't roll the sample of this department, thereby influence the extraction of DNA, consequently, the contained angle between rolling net 6 bottom surface and side is less than 7 bottom surfaces and supporting band 7 contained angles between the side and can guarantee that the sample of supporting the area of both right and left can be fine rolls the breakage.

when the DNA extraction instrument works, the existing DNA extraction instrument is heavy and inconvenient to carry, so that in an outdoor environment, the extraction of DNA samples is complex, particularly in a field environment with various geographical and weather conditions, the DNA of fresh samples is difficult to extract in time, the invention realizes great simplification of equipment on the premise of ensuring the smooth extraction of the DNA of the samples, firstly, a buffer solution (generally Tris-cl solution with the concentration of 0.1M, pH8.0) is placed in a buffer solution storage chamber 3 at the top, a plug 33 is plugged, an extracting solution (generally NAOH solution with the concentration of 0.4) is added into the extracting solution storage chamber 8, the samples are placed between a rolling net 6 and a supporting belt 7, after an upper shell 1 and a lower shell 2 are erected, a coupler 41 at the outer end of a transmission shaft 4 is connected to a motor shaft of a motor, the motor is electrified, the motor rotates, and then a rolling wheel 5 is driven by the transmission shaft 4 to rotate, the rolling wheel 5 is eccentrically arranged on the transmission shaft 4, after the rolling wheel 5 rotates, the rolling wheels 5 on two sides can sequentially extrude the rolling net 6 below, so that the rolling net 6 deforms, and the sample below is rolled and crushed, under the pressure action of the rolling net 6, the supporting belt 7 also deforms, in the process that the supporting belt 7 deforms downwards, the tapered hole 71 in the middle of the supporting belt 7 also deforms, the tapered hole below deforms and is then opened, so that the tapered hole 71 can be opened along with the rolling, the extracting solution below overflows, when the water level of the extracting solution drops to a certain degree, the extracting solution cannot be continuously added, under the action of the extracting solution, the cell wall is gradually crushed, DNA is gradually exposed, after the rolling is finished, the plug 33 of the buffer solution storage chamber 3 is opened, so that the buffer solution flows into the upper shell 1 through the arc-shaped hole 35 from the water through hole 32 and is sprayed on the sample, at the moment, the rolling wheel 5 continues to rotate to accelerate the action of the buffer solution on the sample and accelerate the DNA extraction efficiency, after the extraction is finished, a plug 33 of the buffer solution storage chamber 3 is plugged, the power supply of the motor is turned off, the equipment is inverted, the rolled sample mixed solution is downwards filtered and sprinkled by the rolling net 6, and after the standing is finished, the supernatant is removed to analyze the DNA of the sample.

the foregoing illustrates and describes the principles, general features, and advantages of the present invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are described in the specification and illustrated only to illustrate the principle of the present invention, but that various changes and modifications may be made therein without departing from the spirit and scope of the present invention, which fall within the scope of the invention as claimed. The scope of the invention is defined by the appended claims and equivalents thereof.