阅读说明：本技术 抗体选择方法 (Antibody selection method ) 是由 H·克腾伯格 W·理查特 L·拉里维尔 T·克拉夫特 T·埃姆里希 于 2018-04-25 设计创作，主要内容包括：本文中报道了一种用于选择在食蟹猴中具有小于8mL/kg/天的全身清除率的抗体的方法,所述方法包括步骤：采用阳性线性pH梯度进行FcRn亲和色谱和采用阳性线性电导率/盐梯度上进行肝素亲和色谱,测量抗体的保留时间,并且选择抗体,所述抗体具有比SEQ ID NO:03和04的氧化型抗Her3抗体制备物的峰2和峰3保留时间之间的保留时间差异的1.78倍小的FcRn亲和色谱柱上相对保留时间,和比SEQ ID NO:01和02的抗pTau抗体的保留时间的0.87倍小的肝素亲和色谱柱上相对保留时间。(Herein is reported a method for selecting antibodies having a systemic clearance in cynomolgus monkeys of less than 8 mL/kg/day, said method comprising the steps of: FcRn affinity chromatography with a positive linear pH gradient and heparin affinity chromatography on a positive linear conductivity/salt gradient, the retention times of the antibodies were measured and antibodies were selected having a relative retention time on the FcRn affinity chromatography column that was less than 1.78 times the retention time difference between the retention times of peak 2 and peak 3 of the oxidized anti-Her 3 antibody preparations of SEQ ID NOs 03 and 04 and a relative retention time on the heparin affinity chromatography column that was less than 0.87 times the retention time of the anti-pTau antibodies of SEQ ID NOs 01 and 02.)