阅读说明：本技术 2，6-二羧基吡啶的应用 (Application of 2,6-dicarboxy pyridine ) 是由 拉博德·佩德罗 王苏妍 宋雪歌 于 2019-09-09 设计创作，主要内容包括：本发明提供2,6-二羧基吡啶在制备拮抗植物病原真菌的药物上的应用；所述植物病原真菌包括梨树腐烂病病菌、链格孢菌、葡萄座腔菌或立枯丝核菌的一种或多种；本发明还提供2,6-二羧基吡啶在梨树腐烂病的预防或治疗药物方面的应用；本发明还提供一种拮抗植物病原真菌的药物,其组成成分中至少包含2,6-二羧基吡啶。本发明还提供一种梨树腐烂病预防或治疗药物,其组成成分中至少包含2,6-二羧基吡啶。本发明的2,6-二羧基吡啶所制备药物具有能够非可逆性杀死梨树腐烂病病菌的作用；其中的2,6-二羧基吡啶能够渗透树皮和韧皮部,3mM的2,6-二羧基吡啶能完全抑制梨树腐烂病病菌的生长。(The invention provides application of 2,6-dicarboxy pyridine in preparing medicine for antagonizing plant pathogenic fungi; the plant pathogenic fungi comprise one or more of pear tree rot disease bacteria, alternaria alternate, grape cavity bacteria or rhizoctonia solani; the invention also provides the application of the 2,6-dicarboxy pyridine in the aspect of preventing or treating the pear tree rot; the invention also provides a drug for antagonizing plant pathogenic fungi, which at least comprises 2,6-dicarboxy pyridine. The invention also provides a medicament for preventing or treating the pear tree rot, which comprises at least 2,6-dicarboxy pyridine in the components. The medicine prepared from the 2, 6-dicarboxylpyridine has the effect of irreversibly killing the pathogens of the pear tree rot disease; wherein the 2, 6-dicarboxylpyridine can permeate bark and phloem, and 3mM of the 2, 6-dicarboxylpyridine can completely inhibit the growth of the pyricularia piricola.)

The application of 2,6-dicarboxy pyridine in preparing medicine for antagonizing plant pathogenic fungi is disclosed.

2. Use of 2, 6-dicarboxylpyridines in the preparation of a medicament to antagonize phytopathogenic fungi of the species pyricularia, alternaria, botrytis or rhizoctonia solani.

The application of 2,6-dicarboxy pyridine in preparing the medicine for preventing or treating pear tree rot is disclosed.

4. A medicament for combating phytopathogenic fungi, characterized in that it comprises at least 2, 6-dicarboxylpyridine.

5. A preventive or therapeutic agent for pear tree rot is characterized by comprising at least 2, 6-dicarboxylpyridine as a component.

6. The preventive or therapeutic agent for pear tree rot according to claim 5, comprising Zn²⁺、Co²⁺、Cu²⁺And 2, 6-dicarboxylpyridine.

Technical Field

The invention belongs to the field of biological agriculture, and particularly relates to application of 2, 6-dicarboxylpyridine.

Background

The pear industry is an important industry for producing Chinese fruit trees. In recent years, along with the enlargement of the planting scale of pear trees, the disease of the pear trees is more serious, wherein the rot disease of the pear trees is one of the most serious fungal diseases damaging the pear industry and is called as 'cancer' of the pear trees. Once the disease occurs, a large amount of dead trees and even garden damage can be caused, and great loss is caused to fruit growers. The pear rot disease is a fungal disease, can invade from a wound part of a bark through conidia, is greatly accumulated in a phloem, causes the phloem cells to die, gradually invades a cambium and a xylem, and finally causes the pear tree to die. The best control period for this disease is before the mycelium reaches the cambium, and the difficulty of control is the lack of highly effective fungicides that can effectively penetrate the bark to the pathogenic site.

So far, no special medicament for preventing and treating the disease exists in the market. In production, a method of smearing chemical agents (such as lime sulphur, humic acid copper aqua and the like) after removing scabs is often adopted. Although the methods obtain good control effect, the method needs manual scraping of disease spots, is time-consuming and labor-consuming, and has increasingly prominent problems of pesticide residue and environmental pollution caused by the use of chemical agents. Therefore, the development of the fungicide with low toxicity and environmental protection is of great significance for preventing and treating the pear tree rot and promoting the stable development of the pear industry.

2, 6-dicarboxyphenylpyridine (2, 6-dicarboxyphenylidine, DPA, with the structural formula

2, 6-dicarboxylpyridine is a natural product, is an oxidation degradation product of vitamins, coenzyme and alkaloid, is also an important component of fulvic acid, is an intermediate of a tryptophan degradation pathway and a precursor substance of NAD coenzyme, and has the characteristic of good environmental compatibility. A few reports on the study of 2, 6-dicarboxylpyridine in complexation with copper as an antagonist of Candida (Journal of Thermal Analysis & calibration 2014,115: 2191-352201; Polyandron 2009,28:3526-3532), but the study of 2, 6-dicarboxylpyridine for the control of phytopathogenic fungi remains blank.

Disclosure of Invention

The invention aims to provide application of 2,6-dicarboxy pyridine.

In order to solve the above technical problems, embodiments of the present invention provide an application of 2, 6-dicarboxylpyridine in the preparation of a drug for antagonizing a plant pathogenic fungus.

Further, the plant pathogenic fungi comprise one or more of pyricularia piricola, alternaria alternata, gluconobacter or rhizoctonia solani.

The embodiment of the invention also provides application of the 2,6-dicarboxy pyridine in preparing the medicine for preventing or treating the pear tree rot.

The embodiment of the invention also provides a drug for antagonizing the plant pathogenic fungi, which is characterized in that the components at least comprise 2,6-dicarboxy pyridine.

The embodiment of the invention also provides a medicament for preventing or treating the pear tree rot, which is characterized by comprising at least 2,6-dicarboxy pyridine in the components.

Further, a preventive or therapeutic agent for pear tree rot, comprising Zn²⁺、Co²⁺、Cu²⁺And 2, 6-dicarboxylpyridine.

The technical scheme of the invention has the following beneficial effects: the medicine prepared from the 2, 6-dicarboxylpyridine has the effect of irreversibly killing the pathogens of the pear tree rot disease; wherein the 2, 6-dicarboxylpyridine is capable of penetrating the bark and phloem with half-lives of 15 days and 10 days, respectively; the product is insensitive to light irradiation, and still stably exists after being irradiated by sunlight for 15 days; the 3mM 2,6-dicarboxy pyridine can completely inhibit the growth of the pear tree rotten disease germs; the inhibition ability of the 2,6-dicarboxy pyridine solution to the pear tree rotten disease germ in the pH 1.0-3.0 solution is stronger than that in the pH 4.0-7.0; 2, 6-dicarboxylpyridines with Zn²⁺、Co^{2 +}、Cu²⁺The medicine prepared by ions can enhance the inhibition capability of the pear tree rot pathogen.

Drawings

FIG. 1 is a graph showing the antifungal activity of 2, 6-dicarboxylpyridine against plant pathogenic fungi in example of the present invention;

wherein, FIG. 1A is a graph showing the effect of different concentrations of 2, 6-dicarboxylpyridine on the growth of the mycelia of Pyricularia pyricularia (Valsa ambiens var. pyr. and Valsa mali var. pyr.), Alternaria (Alternaria alternata), Staphylomyces viticola (Botryosphaeria dothidea) and Rhizoctonia solani (Rhizoctonia solani); FIG. 1B is a graph of the growth of Valsa mali var. pyri on PDA solid medium containing 0mM, 1mM, 3mM and 5mM 2, 6-dicarboxylpyridine;

FIG. 2 is a graph of the effect of 2, 6-dicarboxylpyridine solutions of different pH on the inhibition of growth of Valsa mali var. pyri in examples of the present invention;

FIG. 3 shows different complex metal ions (Fe)²⁺、Ni²⁺、Zn²⁺、Co²⁺、Cu²⁺) Graph of the effect on the anti-Valsa mali var. pyri activity of 2, 6-dicarboxylpyridine;

FIG. 4 is a graph of stability and penetration of 2, 6-dicarboxylpyridine in pear tree branches; wherein, FIG. 4A is a graph of stability of 2, 6-dicarboxylpyridine in bark, and FIG. 4B is a graph of stability of 2, 6-dicarboxylpyridine in phloem;

FIG. 5 shows the preventive and therapeutic effects of 2, 6-dicarboxylpyridine on pear tree rot; wherein, FIG. 5A is a statistical data analysis chart of the preventive and therapeutic effects of 2, 6-dicarboxylpyridine on pear tree rot; FIG. 5B is a picture of the events following the prevention or treatment of pear tree rot with 2, 6-dicarboxylpyridine.

Detailed Description

In order to make the technical problems, technical solutions and advantages to be solved by the present invention clearer, the following detailed description is given with reference to specific embodiments.