阅读说明：本技术 有机硒生态氨基酸营养剂及其制备方法 (Organic selenium ecological amino acid nutrient and preparation method thereof ) 是由 郑明德 于 2019-09-23 设计创作，主要内容包括：本发明提供一种有机硒生态氨基酸营养剂及其制备方法,属于饲料原料开发技术领域,是将富含硒元素的毛发、蹄、甲作为原料,再依次向反应罐中搅拌加入食用级纯碱、水反应后,加入酸性的柠檬酸后再酶解、加入液体CM菌种得到本发明有机硒生态氨基酸营养剂。本发明原料来源广泛,降低产物生产成本,且制备方法简单,具有更好的适口性和稳定性。(The invention provides an organic selenium ecological amino acid nutritional agent and a preparation method thereof, belonging to the technical field of feed raw material development. The invention has wide raw material sources, reduces the production cost of the product, has simple preparation method and better palatability and stability.)

1. The preparation method of the organic selenium ecological amino acid nutritional agent is characterized by comprising the following steps:

s1, preparing raw materials: cleaning hair, hoof and nail rich in selenium, and air drying to obtain raw materials for later use;

s2, chelation reaction: injecting water and edible soda ash into a reaction tank, heating the reaction tank to 85-95 ℃, putting the raw materials into the reaction tank, and carrying out stirring chelation reaction for 16-20 hours until the raw materials are pasty to obtain a base material;

s3: adding citric acid into the basic material, stirring, adjusting pH to neutral, adding mixed protease, and treating at 35-40 deg.C for 18-25 hr to obtain organic selenium solution;

s4, uniformly stirring the organic selenium solution, the liquid CM strain and the carbon source, and putting the mixture into a container with vent holes for sealed fermentation for 3-5 days to obtain the organic selenium ecological amino acid nutritional agent.

2. The method for preparing organic selenium ecological amino acid nutrient according to claim 1, wherein in step S1, the raw material is dried to no moisture on the surface.

3. The method for preparing the organic selenium ecological amino acid nutritional agent according to claim 1, wherein in step S2, the mass ratio of the water to the edible soda ash is 10:0.06-0.12, and the mass ratio of the total weight of the water and the edible soda ash to the raw materials is 10: 2-3.

4. The method for preparing the organic selenium ecological amino acid nutritional agent according to claim 3, wherein the mass ratio of the water to the edible soda ash is 10:0.08, and the mass ratio of the total weight of the water to the edible soda ash to the raw materials is 10: 2.5.

5. the method for preparing the organic selenium ecological amino acid nutritional agent according to claim 1, wherein in step S3, the mass ratio of the base material, the citric acid and the mixed protease is 4: 0.5-0.6: 0.002-0.003.

6. The method for preparing organic selenium ecological amino acid nutrient according to claim 1, wherein in step S3, the mixed protease is a composition of keratinase, thiol protease and endopeptidase.

7. The method for preparing the organic selenium ecological amino acid nutrient according to claim 6, wherein the mass ratio of the keratinase, the thiol protease and the endopeptidase is 0.3-0.6: 1: 10.

8. the method for preparing organic selenium ecological amino acid nutrient according to claim 1, wherein in step S4, the mass ratio of the organic selenium solution to the CM species is 10: 0.03-0.04.

9. The method for preparing organic selenium ecological amino acid nutrient according to claim 1, wherein in step S4, the carbon source is brown sugar or molasses; the mass ratio of the organic selenium solution to the carbon source is 10: 0.05-0.08.

10. An organic selenium ecological amino acid nutritional agent obtained by the preparation method of claims 1-9.

Technical Field

The invention relates to the technical field of feed processing, in particular to an organic selenium ecological amino acid nutritional agent and a preparation method thereof.

Background

Selenium is one of essential trace elements for human and animal growth, and after being absorbed by animal, all tissues and cells of body contain selenium, and the content is 20-50 mug/kg body weight. Wherein, the content of the fat is the highest in kidney, liver and fur, the second in muscle, bone and blood and the lowest in fat. Proper amount of selenium has various physiological functions, can improve the immunity of organisms, regulate growth and improve production performance, and has the effects of resisting cancer, oxidation and stress, delaying senility, antagonizing certain toxicants or toxic elements and the like. The selenium element is naturally generated by animals or plants and is the really active organic selenium protein nutrient, so the selenium-containing range of the livestock and poultry meat and eggs obtained by feeding meets the requirement required by the human body by adding the selenium-rich ecological amino acid nutrient into the complete feed, and the selenium-containing range is easier to be absorbed by the human body.

The inventor submits Chinese patent application (application publication number is CN106798078A) in 2016, and discloses a method for extracting selenium-rich nutrient solution from chicken feathers, wherein the method has certain defects, the raw materials are single, keratin is extracted after diluted acid and heating reaction, then the keratin is fermented to obtain nutrient solution, the mental state of partial livestock and poultry fed by the nutrient solution is poor, and the nutrient solution is often diluted, so that the inventor develops the nutrient solution with better adaptability by readjustment.

Disclosure of Invention

In order to solve the problems, the invention provides the organic selenium ecological amino acid nutritional agent and the preparation method thereof.

The technical scheme adopted by the invention is as follows:

a preparation method of an organic selenium ecological amino acid nutritional agent comprises the following steps:

s1, preparing raw materials: cleaning hair, hoof and nail rich in selenium, and air drying to obtain raw materials for later use;

s2, chelation reaction: injecting water and edible soda ash into a reaction tank, heating the reaction tank to 85-95 ℃, putting the raw materials into the reaction tank, and carrying out stirring chelation reaction for 16-20 hours until the raw materials are pasty to obtain a base material;

s3: adding citric acid into the basic material, stirring, adjusting pH to neutral, adding mixed protease, and treating at 35-40 deg.C for 18-25 hr to obtain organic selenium solution;

s4, uniformly stirring the organic selenium solution, the liquid CM strain and the carbon source, and putting the mixture into a container with vent holes for sealed fermentation for 3-5 days to obtain the organic selenium ecological amino acid nutritional agent.

Further, in step S1, the raw material is dried until the surface of the raw material is free of moisture.

Further, in step S2, the mass ratio of the water to the edible soda ash is 10:0.06-0.12, and the mass ratio of the total weight of the water and the edible soda ash to the raw materials is 10: 2-3.

Further, the mass ratio of the water to the edible soda ash is 10:0.08, and the mass ratio of the total weight of the water to the edible soda ash to the raw materials is 10: 2.5.

Further, in step S3, the mass ratio of the base material, citric acid and mixed protease is 4: 0.5-0.6: 0.002-0.003.

Further, in step S3, the mixed protease is a combination of keratinase, thiol protease, and endopeptidase.

Further, the mass ratio of the keratinase, the thiol protease and the endopeptidase is 0.3-0.6: 1: 10.

further, in step S4, the mass ratio of the organic selenium solution to the CM strain is 10: 0.03-0.04.

Further, in step S4, the carbon source is brown sugar or molasses; the mass ratio of the organic selenium solution to the carbon source is 10: 0.05-0.08.

The invention also comprises the organic selenium ecological amino acid nutritional agent obtained by any one of the preparation methods.

The raw materials of the invention, namely edible sodium carbonate (sodium hydroxide), citric acid, keratinase, thiol protease, endopeptidase and CM strain, are all purchased from chemical raw material companies at home and abroad and are directly used without any processing.

The invention has the following beneficial effects:

1. the invention adopts the discarded hair, hoof and nail as raw materials, the raw material sources are wide, not only changing waste into valuables, saving resources and protecting the environment, but also being an inexhaustible selenium resource, the extracted nutrient is rich in natural active organic selenoprotein, is safest, has no toxic or side effect, has the most obvious clinical effect and the highest absorption rate, is not limited by crowds and dosage, is widely applied to livestock and poultry breeding, and realizes the aims of supplementing selenium for the whole people and healthy China; can effectively improve the quality of livestock and poultry and improve the economic benefit.

2. According to the invention, the raw materials are firstly subjected to decomposition treatment of edible sodium carbonate, namely sodium hydroxide, so as to obtain the pasty base material, and the method can overcome the defects of high-temperature acidolysis treatment and high equipment requirement in the traditional method, and can better decompose the raw materials.

3. The invention adopts the dual functions of mixed protease and CM strain, which can effectively improve the gastrointestinal tract function of livestock and poultry, enhance the disease resistance, and completely and thoroughly replace the function of antibiotic drugs in feed; the raw materials are subjected to enzymolysis treatment by mixed protease to obtain an organic selenium solution, a CM strain and a carbon source are added into the organic selenium solution for fermentation treatment, the content of the obtained natural active organic selenium protein is 3-4 times that of the natural active organic selenium protein which is not fermented by the CM strain, the selenium existing form is quickly converted into the organic selenium protein after the CM strain is treated, the absorption efficiency of livestock and poultry can be greatly improved, and the selenium content of the meat and eggs of the livestock and poultry which are fed reaches the internationally established standard.

Detailed Description

The following examples may assist those skilled in the art in a more complete understanding of the present invention, but are not intended to limit the invention in any way.