阅读说明：本技术 节节麦hmt1基因的应用 (Application of arthroncus sylvestris HMT1 gene ) 是由 张波 张怀刚 吴丽军 刘宝龙 刘韬 周让让 陈红 于 2019-10-24 设计创作，主要内容包括：本发明涉及节节麦HMT1基因的应用,属于基因功能鉴定技术领域。本发明提供了节节麦HMT1基因在提高植物抗逆能力、总抗氧化能力、硒耐受能力以及植物硒吸收和累积能力中的应用。节节麦HMT1基因能够提高植物抗逆能力、总抗氧化能力、硒耐受能力并能够提高植物硒吸收和累积能力。(The invention relates to application of a arthroncus evansi HMT1 gene, belonging to the technical field of gene function identification. The invention provides application of arthroncus sylvestris HMT1 gene in improving stress resistance, total antioxidant capacity, selenium tolerance capacity and selenium absorption and accumulation capacity of plants. The arthroncus arvensis HMT1 gene can improve plant stress resistance, total antioxidant capacity and selenium tolerance and can improve plant selenium absorption and accumulation capacity.)

1. Application of arthroncus sylvestris HMT1 gene in improving stress resistance of plants.

2. Application of arthroncus sylvestris HMT1 gene in improving total antioxidant capacity of plants.

3. Application of arthroncus evansi HMT1 gene in improving selenium tolerance of plants.

4. Application of arthroncus evansi HMT1 gene in improving selenium absorption and accumulation capacity of plants.

5. The use according to any one of claims 1 to 4, wherein the plant comprises tobacco.

6. Application of arthroncus evansi HMT1 gene in cultivating selenium-rich plants.

7. Application of arthroncus sylvestris HMT1 gene in cultivation of selenium-enriched wheat.

Technical Field

The invention relates to the technical field of gene function identification, in particular to application of a arthroncus evansi HMT1 gene.

Background

Selenocysteine Methyltransferase (SMT) is believed to be a methylselenocysteine methyltransferase that specifically catalyzes the production of non-protein by selenocysteine (secs) with methanol methyl, thereby reducing the intracellular concentrations of selenocysteine and selenomethionine. The homology of the SMT protein of Astragalus (Astragalus bisultatus) and the Escherichia coli yagD is more than 40%, and the latter protein has the capability of catalyzing homocysteine, selenocysteine and selenocysteine by methyl.

Comparing the SMT amino acid sequence of astragalus root in each database, the similarity of the sequence and the Homocysteine Methyltransferase (HMT, EC 2.1.1.10) of arabidopsis thaliana and rice reaches 70 percent and 65 percent respectively. Furthermore, cDNA clones of Arabidopsis HMT were obtained by functional complementation experiments of E.coli yagD mutant. Homocysteine Methyltransferase (HMT) catalyzes methionine synthesis from homocysteine with methyl methionine as a methyl donor. Collectively, these results indicate that SMT and HMT have similar structure and function, and both have the ability to catalyze methylcysteine and homocysteine and their selenium isomorphs, with differing specificities.

The HMT gene and the SMT gene are derived from a common ancestral gene. SMT and HMT are highly consistent, can catalyze cysteine to be methyl cysteine, have different catalytic activities and play different roles in the metabolic process of plant sulfur and selenium. The sequence of the arthroncus HMT1 gene has been published, but the functional study of the gene has not been reported.

Disclosure of Invention

The invention aims to provide application of the arthroncus evansi HMT1 gene. The arthroncus arvensis HMT1 gene can improve plant stress resistance, total antioxidant capacity and selenium tolerance and can improve plant selenium absorption and accumulation capacity.

The invention provides application of arthroncus sylvestris HMT1 gene in improving stress resistance of plants.

The invention also provides application of the arthroncus sylvestris HMT1 gene in improving the total antioxidant capacity of plants.

The invention also provides application of the arthroncus sylvestris HMT1 gene in improving selenium tolerance of plants.

The invention also provides application of the arthroncus sylvestris HMT1 gene in improving selenium absorption and accumulation capacity of plants.

Preferably, the plant comprises tobacco.

The invention also provides application of the arthroncus evansi HMT1 gene in cultivation of selenium-rich plants.

The invention also provides application of the arthroncus william HMT1 gene in cultivation of selenium-enriched wheat.

The invention provides application of a arthroncus evansi HMT1 gene. The arthroncus arvensis HMT1(AetHMT1) gene can improve the stress resistance, total antioxidant capacity and selenium tolerance of plants and can improve the selenium absorption and accumulation capacity of the plants; the AetHMT1 gene is related to plant selenium metabolism, can improve the absorption and accumulation capacity of plant selenium, takes the Jiegeng wheat HMT1 as an effective selenium resource, and can synthesize selenium-rich wheat by a genetic breeding method to meet the daily selenium nutrition of people so as to reduce the cancer risk of people.

Drawings

FIG. 1 is a schematic diagram of the route for the genetic transformation of the gene HMT1 into tobacco by Agrobacterium tumefaciens LBA4404 mediated transformation provided by the present invention;

FIG. 2 is a schematic diagram of the construction of the plant expression vector of AetHMT1 gene provided by the present invention;

FIG. 3 is a diagram showing the results of colony PCR screening of the plant expression vector pBI-XS HMT1 provided by the present invention;

FIG. 4 is a diagram of the tissue culture process of the tobacco with the gene AetHMT1 provided by the invention;

FIG. 5 is a diagram showing the result of PCR detection of the DNA level of the tobacco transformed with AetHMT1 gene according to the present invention;

FIG. 6 is a total RNA electrophoresis chart of a tobacco sample with transferred AetHMT1 gene provided by the invention;

FIG. 7 is a diagram showing the result of PCR detection of the transcription level of the tobacco transformed with AetHMT1 gene according to the present invention;

FIG. 8 is a diagram showing the flowering and seed acquisition of the AetHMT1 transgenic tobacco provided by the present invention;

FIG. 9 is a graph showing the effect of AetHMT1 on tobacco moisture content under selenium stress according to the present invention;

FIG. 10 is a graph showing the effect of AetHMT1 on tobacco MDA content under selenium stress;

FIG. 11 is a graph showing the effect of AetHMT1 on the T-AOC capacity of tobacco treated with selenium according to the present invention;

FIG. 12 is a graph showing the effect of AetHMT1 on tobacco POD activity under selenium stress according to the present invention;

FIG. 13 is a graph showing the effect of AetHMT1 on the total selenium content of tobacco treated with selenium according to the present invention.

Detailed Description

The invention provides application of arthroncus sylvestris HMT1 gene in improving stress resistance of plants.

The invention also provides application of the arthroncus sylvestris HMT1 gene in improving the total antioxidant capacity of plants. The T-AOC capability of the tobacco with the transferred AetHMT1 gene is higher than that of wild tobacco.

The invention also provides application of the arthroncus sylvestris HMT1 gene in improving selenium tolerance of plants.

The invention also provides application of the arthroncus sylvestris HMT1 gene in improving selenium absorption and accumulation capacity of plants. The total selenium content of the tobacco with the transferred AetHMT1 gene is higher than that of the wild tobacco by three liters.

In the present invention, the plant comprises tobacco.

The invention also provides application of the arthroncus evansi HMT1 gene in cultivation of selenium-rich plants.

The invention also provides application of the arthroncus william HMT1 gene in cultivation of selenium-enriched wheat.

The application of the arthroncus wheat HMT1 gene of the present invention will be described in further detail with reference to the following specific examples, and the technical solution of the present invention includes, but is not limited to, the following examples.