阅读说明：本技术 一种用于同时提取氟苯尼考、氯霉素、甲砜霉素的免疫亲和柱 (It is a kind of for extracting the immune affinity column of Florfenicol, chloramphenicol, Thiamphenicol simultaneously ) 是由 武玉香 沈志强 崔平 于 2019-09-04 设计创作，主要内容包括：本发明实施例公开了一种用于同时提取氟苯尼考、氯霉素、甲砜霉素的免疫亲和柱,所述免疫亲和柱包括一空心柱体,所述空心柱体的一端设有第一盖体,另一端设有第二盖体,在所述空心柱体靠近所述第二盖体内部填充有偶联氟苯尼考单克隆抗体的固相介质,在所述固相介质的上表面上设有第一筛板,在所述固相介质的下表面上设有第二筛板。本发明实施例公开了一种可同时提取氟苯尼考、氯霉素、甲砜霉素的免疫亲和柱,其中在亲和柱中填充有偶联有氟苯尼考抗体的固相介质,可同时检测样本中氟苯尼考、氯霉素、甲砜霉素残留,取得了意想不到的技术效果。(The embodiment of the invention discloses a kind of for extracting the immune affinity column of Florfenicol, chloramphenicol, Thiamphenicol simultaneously, the immune affinity column includes a hollow cylinder, one end of the hollow cylinder is equipped with the first lid, the other end is equipped with the second lid, solid-phase media in the hollow cylinder close to second lid portion inside filled with coupling Florfenicol monoclonal antibody, it is equipped with the first sieve plate on the upper surface of the solid-phase media, the second sieve plate is equipped on the lower surface of the solid-phase media.The embodiment of the invention discloses the immune affinity columns that one kind can extract Florfenicol, chloramphenicol, Thiamphenicol simultaneously, wherein there is the solid-phase media of Florfenicol antibody filled with coupling in affinity column, Florfenicol in sample, chloramphenicol, Thiamphenicol residual can be detected simultaneously, achieve unexpected technical effect.)

1. a kind of for extracting the immune affinity column of Florfenicol, chloramphenicol, Thiamphenicol simultaneously, which is characterized in that described to exempt from Epidemic disease affinity column includes a hollow cylinder, and one end of the hollow cylinder is equipped with the first lid, and the other end is equipped with the second lid, in institute Solid-phase media of the hollow cylinder close to second lid portion inside filled with coupling Florfenicol monoclonal antibody is stated, described solid The upper surface of phase medium is equipped with the first sieve plate, and the second sieve plate is equipped on the lower surface of the solid-phase media.

2. the immune affinity column as described in claim 1 being used for while extracting Florfenicol, chloramphenicol, Thiamphenicol, special Sign is,

The Florfenicol monoclonal antibody is prepared by Florfenicol FF3C11 cell strain, the Florfenicol FF3C11 cell strain is preserved on July 4th, 2019 positioned at Wuhan, China Wuhan University China typical culture collection center, Deposit number is CTCC NO:C2019152.

3. the immune affinity column as claimed in claim 2 being used for while extracting Florfenicol, chloramphenicol, Thiamphenicol, special Sign is,

The Florfenicol monoclonal antibody is to be obtained by Florfenicol hapten-carrier protein conjugate as immunogene preparation .

4. the immune affinity column as claimed in claim 3 being used for while extracting Florfenicol, chloramphenicol, Thiamphenicol, special Sign is,

The carrier protein is bovine serum albumin(BSA), ovalbumin, perforation hemocyanin or human serum albumins.

5. the immune affinity column as described in claim 1 being used for while extracting Florfenicol, chloramphenicol, Thiamphenicol, special Sign is,

First cover board and second cover board are all made of plastic material and are made；

First sieve plate and the second sieve plate are all made of water wetted material and are made.

6. the immune affinity column as described in claim 1 being used for while extracting Florfenicol, chloramphenicol, Thiamphenicol, special Sign is,

The diameter of the hollow cylinder is 2cm, is highly 10cm.

7. the immune affinity column as described in claim 1 being used for while extracting Florfenicol, chloramphenicol, Thiamphenicol, special Sign is,

The solid-phase media includes agarose 4FF medium.

8. the immune affinity column as described in claim 1 being used for while extracting Florfenicol, chloramphenicol, Thiamphenicol, special Sign is,

The ethyl alcohol for being 20% filled with mass fraction in the hollow cylinder saves liquid.

Technical field

The present embodiments relate to technical field of immunoassay, and in particular to for simultaneously extract Florfenicol, chloramphenicol, The immune affinity column of Thiamphenicol.

Background technique

Chloromycetin series antibiotics specifically include that Florfenicol, chloramphenicol, Thiamphenicol, Florfenicol are amphenicols the 3rd For animal specific antimicrobial, there is wide spectrum, efficient, good absorbing, be distributed in vivo extensively, without spies such as induced aplastic anemia effects Point, the alternative medicine as chloramphenicol are widely used in treating the bacteriosis of livestock and poultry.Studies have shown that Florfenicol, chlorine are mould Element, Thiamphenicol have embryotoxicity and drug resistance, and the extensive application in Production of Livestock and Poultry also causes it residual in animal products It stays, endangers public health.For the liquid chromatographic detection of chloromycetin series antibiotics, sample acquisition and pre-treatment are to divide at present Bottleneck in analysis method, sample-pretreating method step is various, is divided into extraction and purification 2 big steps, especially purifying step More, it is easy to cause error, within error rate 20%, operating process is loaded down with trivial details, complicated, the time is long, large labor intensity, to operator Member's injury is larger.

Therefore, it is necessary to how research and develop a kind of easy to operate and high chloromycetin series antibiotics immune affinity column of purification efficiency, The chloromycetin series antibiotics in sample to be examined are separated and purified using chloromycetin series antibiotics specific antibody, are urgently The technical issues of solution.

Summary of the invention

For this purpose, the embodiment of the present invention provide it is a kind of for and meanwhile extract the immune parent of Florfenicol, chloramphenicol, Thiamphenicol And column, to solve to extract in the prior art, separation Florfenicol, chloramphenicol, Thiamphenicol process are complicated, and purification efficiency is low to ask Topic.

To achieve the goals above, the embodiment of the present invention provides the following technical solutions:

It is a kind of for extracting the immune affinity column of Florfenicol, chloramphenicol, Thiamphenicol, the immune affinity column simultaneously Including a hollow cylinder, one end of the hollow cylinder is equipped with the first lid, and the other end is equipped with the second lid, in the open tubular column Solid-phase media of the body close to second lid portion inside filled with coupling Florfenicol monoclonal antibody, in the solid-phase media Upper surface is equipped with the first sieve plate, and the second sieve plate is equipped on the lower surface of the solid-phase media.

Preferably, the Florfenicol monoclonal antibody is prepared by Florfenicol FF3C11 cell strain, The Florfenicol FF3C11 cell strain has been preserved on July 4th, 2019 to be trained positioned at Wuhan, China Wuhan University Chinese Typical Representative Object collection is supported, deposit number is CTCC NO:C2019152.

Preferably, the Florfenicol monoclonal antibody is to be used as to exempt from by Florfenicol hapten-carrier protein conjugate Epidemic focus prepares.

Preferably, the carrier protein is bovine serum albumin(BSA), ovalbumin, perforation hemocyanin or human seralbumin egg It is white.

Preferably, first cover board and second cover board are all made of plastic material and are made；

First sieve plate and the second sieve plate are all made of water wetted material and are made.

Preferably, the diameter of the hollow cylinder is 2cm, is highly 10cm.

Preferably, the solid-phase media includes agarose 4FF medium.

Preferably, the ethyl alcohol for being 20% filled with mass fraction in the hollow cylinder saves liquid.

The embodiment of the present invention has the advantages that

The embodiment of the invention discloses one kind can extract the immune affinity column of Florfenicol, chloramphenicol, Thiamphenicol simultaneously, Wherein there is the solid-phase media of Florfenicol antibody filled with coupling in affinity column, Florfenicol in sample, chlorine can be detected simultaneously Mycin, Thiamphenicol residual, achieve unexpected technical effect.Immune affinity column provided by the present invention has operation letter The features such as list, high sensitivity, detection speed fast, at low cost, it is suitble to honey, meat, egg, milk, feed, serum, feed, egg etc. more The detection of kind sample.The immune affinity column of the embodiment of the present invention uses specific immune response for separation means, substantially increases The purity and purification efficiency of sample, ensure that the reliability and stability of testing result.The embodiment of the present invention uses diameter 2cm, High 10cm hollow cylinder, increases active area, the pressure for saving solution is greatly reduced, so that the aperture of medium is not by pressure It influences and becomes smaller, to not influence flow velocity, avoid and drained using pressure pump device help, provided just to the detection of sample Benefit.

Detailed description of the invention

It, below will be to embodiment party in order to illustrate more clearly of embodiments of the present invention or technical solution in the prior art Formula or attached drawing needed to be used in the description of the prior art are briefly described.It should be evident that the accompanying drawings in the following description is only It is merely exemplary, it for those of ordinary skill in the art, without creative efforts, can also basis The attached drawing of offer, which is extended, obtains other implementation attached drawings.

Fig. 1 is the structural schematic diagram of immune affinity column provided in an embodiment of the present invention；

Fig. 2 is Florfenicol monoclonal antibody Purity figure provided in an embodiment of the present invention.

In figure: 100- hollow cylinder；The first lid of 110-；The second lid of 120-；The first sieve plate of 130-；140- second is sieved Plate；150- saves liquid.

Specific embodiment

Embodiments of the present invention are illustrated by particular specific embodiment below, those skilled in the art can be by this explanation Content disclosed by book is understood other advantages and efficacy of the present invention easily, it is clear that described embodiment is the present invention one Section Example, instead of all the embodiments.Based on the embodiments of the present invention, those of ordinary skill in the art are not doing Every other embodiment obtained under the premise of creative work out, shall fall within the protection scope of the present invention.