阅读说明：本技术 便携式背向照明光学成像显微镜 (Portable back-carrying is to light optics imaging microscope ) 是由 侯国利 侯国辉 刘友兰 侯利艳 于 2019-09-23 设计创作，主要内容包括：本发明涉及显微镜的技术领域,公开了便携式背向照明光学成像显微镜,包括物镜、Z方向位移控制模块、XY方向位移控制模块、背向照明模块、成像单元转接模块、成像单元以及目镜；本发明提供的便携式背向照明光学成像显微镜避开了传统科勒照明光源结构,光源不需要通过二次成像的像做光源进行样品照明,而是直接选用具有面光源特性的LED作为光源采用轴向位移控制的模块进行直接背照方式照明。这样便缩短了原来需要采用科勒照明光路结构引入的距离,使得显微镜的结构与体积进一步减小。因此,这种设计采用平面LED光源作为照明系统,大大降低整体系统的成本和占用空间。(The present invention relates to microscopical technical fields, disclose Portable back-carrying to light optics imaging microscope, including object lens, Z-direction Bit andits control module, the direction XY displacement control module, backwards lighting module, imaging unit interconnecting module, imaging unit and eyepiece；Portable back-carrying provided by the invention avoids traditional kohler's illumination light-source structure to light optics imaging microscope, light source does not need to do light source progress sample illumination by the picture of secondary imaging, but the module that directly there is the LED of area source characteristic to be controlled as light source using axial displacement for selection carries out direct back-illuminated mode and illuminates.The distance for needing to introduce originally using kohler's illumination light channel structure is just shortened in this way, so that microscopical structure is further decreased with volume.Therefore, this design substantially reduces the cost and occupied space of total system using planar LED light source as lighting system.)

1. Portable back-carrying is to light optics imaging microscope, which is characterized in that including object lens, Z-direction Bit andits control module, the side XY To Bit andits control module, backwards to lighting module, imaging unit interconnecting module, imaging unit and eyepiece；The Z-direction displacement control Molding block is for controlling the object lens and the distance between sample to be observed, and the direction the XY displacement control module is for controlling The position of the object lens observation visual field；It is described to include half-plane LED illumination light source, be divided plate, for pacifying backwards to lighting module It fills the half-plane LED illumination light source and is divided the mounting fixing parts and displacement slot of plate, the mounting fixing parts are slidably It is set in the displacement slot；The imaging unit is for receiving optical signal and generating picture number according to the received optical signal of institute According to；The imaging unit interconnecting module includes slidable reflective mirror；Wherein, the light that the half-plane LED light source issues passes through The light splitting plate is reflected into the object lens, irradiates sample to be observed, and sample to be observed reflects light back into object lens, then The first light is formed transmitted through the light splitting plate, it is described when the reflective mirror slides into the opposite place of first light First light is imaged through the mirror reflection to the imaging unit；When the reflective mirror slides into other positions, First light is directly entered eyepiece for human eye observation.

2. Portable back-carrying as described in claim 1 is to light optics imaging microscope, which is characterized in that the imaging unit turns Connection module further includes adapter, and the adapter includes horizontally disposed first light channel and vertically arranged second light Channel, the middle part of first light channel are connected to the middle part of second light channel, first light channel and institute It is positioned opposite to state eyepiece, second light channel and the imaging unit are positioned opposite；The adapter has sliding slot, described Reflective mirror is slidably set in the sliding slot.

3. Portable back-carrying as described in claim 1 is to light optics imaging microscope, which is characterized in that the direction the XY displacement Control module include along the x axis screw rod and Y direction screw rod, the X-direction screw rod arrange along the x axis, the X-axis Direction screw rod realizes the object lens X-direction displacement for abutting the object lens, and the Y direction screw rod is arranged along the y axis, The Y direction screw rod realizes the object lens Y-direction displacement for abutting the object lens.

4. Portable back-carrying as described in claim 1 is to light optics imaging microscope, which is characterized in that the Z-direction displacement Control module includes the screw rod along Z-direction arrangement, and the screw rod is threadedly coupled with the object lens, when the rotation screw rod, institute Object lens are stated to move along Z-direction.

5. Portable back-carrying as described in claim 1-4 any one is to light optics imaging microscope, which is characterized in that described Imaging unit includes imaging sensor, converter, single-chip microcontroller, display, in which: the imaging sensor is used for optical signal It is converted into electric signal and is transmitted to the converter；The converter is for handling the electricity sent by the imaging sensor Signal, and the single-chip microcontroller is sent to after the electric signal is converted to digital signal；The single-chip microcontroller is for encoding by the AD The digital signal that converter is sent obtains image data.

6. Portable back-carrying as claimed in claim 5 is to light optics imaging microscope, which is characterized in that the imaging sensor For ccd sensor.

7. Portable back-carrying as claimed in claim 5 is to light optics imaging microscope, which is characterized in that the imaging sensor For cmos sensor.

8. Portable back-carrying as claimed in claim 5 is to light optics imaging microscope, which is characterized in that the Portable back-carrying to Light optics imaging microscope includes the communication module for establishing communication connection with terminal, the communication module and the monolithic Machine connection.

9. Portable back-carrying as claimed in claim 5 is to light optics imaging microscope, which is characterized in that the periphery of the object lens It is equipped with resilient cushion.

Technical field

The present invention relates to microscopical technical field, especially Portable back-carryings to light optics imaging microscope.

Background technique

Ordinary optical imaging microscope refers to that the optical imagery microscope illuminated using traditional visible light, light source are had Not in fluorescence imaging microscope and nonlinear imaging microscope.The imaging illumination mode and purposes of ordinary optical imaging microscope are situated between It continues as follows:

There are mainly two types of lighting methods, first is that forward direction transmission-type illumination (irradiation light penetrates sample, into object lens), second is that back To reflective (irradiation light exposes to sample by object lens, and light returns again to object lens after the sample reflects) optical microscopy of illumination.

Purposes and technical pattern and technique corresponding to them:

What the principle that the technology that two kinds of lighting methods use is all based on Kohler illumination carried out, so-called Kohler illumination, it is The picture of filament is used to carry out sample illumination as secondary souce.Purpose is to eliminate to carry out on sample using filament direct imaging Illumination unevenness caused by illuminating.It is secondary illumination using basic principle be wavelet superposition principle carry out, i.e., in filament image planes Each point carry out secondary light emitting as point light source, it then follows the principle of wavelet superposition is illuminated, and the benefit done so is just It is illumination at sample is substantially uniform illumination.

It is mainly biomicroscope that forward direction transmission-type, which illuminates microscope, is mainly that metallographic is aobvious backwards to reflecting microscope is illuminated Micro mirror.

Problems and shortcomings existing for two kinds of lighting methods:

Using the microscope of Kohler illumination mode, volume is relatively large, and price is relatively high, and is unfavorable for portable monomer Operation.Although also there is the transmitted light microscope for using LED as luminaire inexpensive instantly, volume is slightly larger, is unfavorable for It carries, and the influence vulnerable to sample transparency.That is, existing optical imagery microscope inexpensively generallys use just To transmission-type lighting method, the sample of transparent sample preparation production imaging is used.It is this for special opaque sample Imaging microscope is outdated.

Optical tube length and the displacement mode for adjusting sample, cause microscope not portable, it appears and it is very heavy, meanwhile, it shows The enlargement ratio of micro mirror can be because used object lens and magnification of ocular be smaller and limited.Optical imagery microscope be by object lens with The imaging organism that lighting source is constituted, so, it is bound to meet optical imagery rule.And traditional Photobiology imaging is micro- Mirror is mainly transmission-type illumination, and leading to the sample of observation is essentially all transparent film-making；Although metallographic microscope mirror is backwards Imaging mode is illuminated, but the lighting source used is halogen lamp or tungsten halogen lamp, this lighting method, which is all strangled by traditional section, shines The influence of bright image optics principle (picture of lighting source filament is as light source illumination sample), cause the size of imaging microscope compared with Greatly, price is also higher.

Summary of the invention

The purpose of the present invention is to provide Portable back-carryings to light optics imaging microscope, it is intended to which solution is shown in the prior art Micro mirror has a single function, not Portable belt the problem of

The invention is realized in this way Portable back-carrying is controlled to light optics imaging microscope, including the displacement of object lens, Z-direction Molding block, the direction XY displacement control module, backwards lighting module, imaging unit interconnecting module, imaging unit and eyepiece；It is described Z-direction Bit andits control module is for controlling the object lens and the distance between sample to be observed, the direction XY Bit andits control Module is used to control the position of the object lens observation visual field；It is described to include half-plane LED illumination light source, be divided backwards to lighting module Plate, mounting fixing parts and displacement slot for installing the half-plane LED illumination light source and light splitting plate, the installation are solid Determine part to be slidably set in the displacement slot；The imaging unit is for receiving optical signal and according to the received optical signal life of institute At image data；The imaging unit interconnecting module includes slidable reflective mirror；Wherein, the half-plane LED light source issues Light be reflected into the object lens by the light splitting plate, irradiate sample to be observed, sample to be observed reflects light Object lens are returned, then form the first light transmitted through the light splitting plate, when the reflective mirror slides into the opposite of first light When place, first light is imaged through the mirror reflection to the imaging unit；When the reflective mirror slides into it When his position, first light is directly entered eyepiece for human eye observation.

Further, the imaging unit interconnecting module further includes adapter, and the adapter includes horizontally disposed One light channel and vertically arranged second light channel, the middle part of first light channel and second light channel Middle part connection, first light channel and the eyepiece are positioned opposite, second light channel and the imaging unit It is positioned opposite；The adapter has sliding slot, and the reflective mirror is slidably set in the sliding slot.

Further, the direction the XY displacement control module includes screw rod and Y direction screw rod along the x axis, the X Axis direction screw rod arranges that the X-direction screw rod is for abutting the object lens to realize object lens X-direction position along the x axis It moves, the Y direction screw rod is arranged along the y axis, and the Y direction screw rod is for abutting the object lens to realize the object lens Y-direction displacement.

Further, the Z-direction Bit andits control module include along Z-direction arrangement screw rod, the screw rod with it is described Object lens connection, when rotating the screw rod, the object lens are moved along Z-direction.

Further, the imaging unit includes imaging sensor, converter, single-chip microcontroller, display, in which: described Imaging sensor is for converting optical signal into electric signal and being transmitted to the converter；The converter for handle by The electric signal that the imaging sensor is sent, and the single-chip microcontroller is sent to after the electric signal is converted to digital signal；It is described Single-chip microcontroller obtains image data by the digital signal that the converter is sent for encoding.

Further, the imaging sensor is ccd sensor.

Further, the imaging sensor is cmos sensor.

Further, the Portable back-carrying to light optics imaging microscope include for establishing communication connection with terminal Communication module, the communication module are connect with the single-chip microcontroller.

Further, the peripheral ring of the object lens is equipped with resilient cushion.

Compared with prior art, Portable back-carrying provided by the invention avoids traditional section to light optics imaging microscope and strangles Lighting light source structure, light source does not need to do light source progress sample illumination by the picture of secondary imaging, but directly selecting has face The LED of light source characteristic carries out direct back-illuminated mode as light source and illuminates.It just shortens and needs originally using kohler's illumination light in this way The distance that line structure introduces, so that microscopical structure further decreases.Therefore, this design uses the conduct of planar LED light source Lighting system substantially reduces the cost and occupied space of total system.

Detailed description of the invention

Fig. 1 is formula provided in an embodiment of the present invention of taking backwards to the stereoscopic schematic diagram of light optics imaging microscope；

Fig. 2 is formula provided in an embodiment of the present invention of taking backwards to the decomposition diagram of light optics imaging microscope；

Fig. 3 is the decomposition diagram of imaging unit interconnecting module and imaging unit provided in an embodiment of the present invention；

Fig. 4 is the decomposition diagram provided in an embodiment of the present invention backwards to lighting module.

Specific embodiment

In order to make the objectives, technical solutions, and advantages of the present invention clearer, with reference to the accompanying drawings and embodiments, right The present invention is further elaborated.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, and It is not used in the restriction present invention.

The same or similar label correspond to the same or similar components in the attached drawing of the present embodiment；In description of the invention In, it is to be understood that if there is the orientation or positional relationship of the instructions such as term " on ", "lower", "left", "right" for based on attached drawing institute The orientation or positional relationship shown, is merely for convenience of description of the present invention and simplification of the description, rather than the dress of indication or suggestion meaning It sets or element must have a particular orientation, be constructed and operated in a specific orientation, therefore describe the use of positional relationship in attached drawing Language only for illustration, should not be understood as the limitation to this patent, for the ordinary skill in the art, can be with The concrete meaning of above-mentioned term is understood as the case may be.

Realization of the invention is described in detail below in conjunction with specific embodiment.

Referring to Fig.1 shown in -4, preferred embodiment is provided for the present invention.

Portable back-carrying is to light optics imaging microscope, including object lens 1, Z-direction Bit andits control module, the displacement control of the direction XY Molding block 2, backwards lighting module 5, imaging unit interconnecting module 6, imaging unit 3 and eyepiece 4；Z-direction Bit andits control module For controlling object lens 1 and the distance between sample to be observed, the direction XY displacement control module 2 is for controlling the observation view of object lens 1 The position of field；It include half-plane LED illumination light source 51, light splitting plate 52, for installing half-plane LED photograph backwards to lighting module 5 The mounting fixing parts 53 and displacement slot 54 in Mingguang City source 51 and light splitting plate 52, mounting fixing parts 53 are slidably set to displacement slot In 54；Imaging unit 3 is for receiving optical signal and generating image data according to the received optical signal of institute；Imaging unit interconnecting module 6 include slidable reflective mirror 62；Wherein, the light that half-plane LED light source issues is reflected into object lens by light splitting plate 52 1, sample to be observed is irradiated, sample to be observed reflects light back into object lens 1, then forms the first light transmitted through light splitting plate 52 Line, when reflective mirror 62 slides into the opposite place of the first light, the first light through reflective mirror 62 reflex to imaging unit 3 carry out at Picture；When reflective mirror 62 slides into other positions, the first light is directly entered eyepiece 4 for human eye observation.

Image-forming principle of the Portable back-carrying of above-mentioned offer to light optics imaging microscope are as follows: half-plane LED light source issues Light be reflected into object lens 1 by light splitting plate 52, irradiate sample to be observed, sample to be observed reflects light back into object lens 1, then the first light, when reflective mirror 62 to be slid into the opposite position of the first light, first are formed transmitted through light splitting plate 52 Light reflexes to imaging unit 3 through reflective mirror 62 and is imaged, and when reflective mirror 62 slides into other positions, the first light is direct Into eyepiece 4 for human eye observation.

Traditional Photobiology imaging microscope is mainly transmission-type illumination, and it is essentially all transparent for leading to the sample of observation Film-making；Although metallographic microscope mirror is backwards to illumination imaging mode, but the lighting source used is halogen lamp or tungsten halogen lamp, this Kind lighting method is all by traditional kohler's illumination image optics principle (picture of lighting source filament illuminates sample as light source) Influence.Cause the size of imaging microscope larger, price is also higher.

The present invention be directed to conventional microscopes due to the drawbacks of being generated using Kohler illumination structure, invent this Portable back-carrying To light optics imaging microscope.It is sent out solving displacement with portable dimension and observation these paradox of Iimaging Stability Bright Creative Design.

Portable back-carrying provided by the invention avoids traditional kohler's illumination light-source structure, light to light optics imaging microscope Source does not need to do light source progress sample illumination by the picture of secondary imaging, but directly selecting, there is the LED of area source characteristic to make Direct back-illuminated mode is carried out for light source to illuminate.Just shorten so originally need using kohler's illumination light channel structure introduce away from From so that microscopical structure further decreases.Therefore, this design is dropped significantly using planar LED light source as lighting system The cost and occupied space of low total system.

The explanation of the image-forming principle of this imaging system: acting on object in uniform source of light, secondary unrestrained on body surface It penetrates light to be imaged by lens group, image-forming principle also meets geometric optics Gauss image-forming principle.

Core of the invention technology is mainly reflected in lighting method and breaches traditional optical imagery microscope by section's Le The limitation of illumination imaging systems light source.Use relatively inexpensive face element formula white light LEDs as light source, so that core is more honest and cleaner Valence, to improve this portable microscope in the convenience of field work.

Specifically, imaging unit interconnecting module 6 further includes adapter 61, and adapter 61 includes horizontally disposed first light Channel 612 and vertically arranged second light channel 613, the middle part of the first light channel 612 and the second light channel 613 Middle part connection, the first light channel 612 and eyepiece 4 are positioned opposite, and the second light channel 613 and imaging unit 3 are positioned opposite；Turn Device 61 is connect with sliding slot 611, reflective mirror 62 is slidably set in sliding slot 611；In this way, when making reflective mirror 62 slide into the first light When the opposite place of line, the first light reflexes to imaging unit 3 through reflective mirror 62 and is imaged；When making reflective mirror 62 slide into other When position, the first light is directly entered eyepiece 4 for human eye observation.

Specifically, the direction XY displacement control module 2 includes screw rod 21 and Y direction screw rod 22 along the x axis, X-axis side It is arranged along the x axis to screw rod 21, X-direction screw rod 21 is for abutting object lens 1, and Y direction screw rod 22 is arranged along the y axis, Y Axis direction screw rod 22 is for abutting object lens 1；In this way by screwing X-direction screw rod 21, it can control object lens 1 and move along the x axis It is dynamic, it can control object lens 1 by screwing Y direction screw rod 22 and move along the y axis, may be implemented by XY direction controlling module Object lens 1 along the x axis with the movement of Y direction, and then realize object lens 1 observation visual field position control.

Furthermore Z-direction Bit andits control module includes the screw rod along Z-direction arrangement, and screw rod is threadedly coupled with object lens 1, when Rotary screw rod, object lens 1 are moved along Z-direction；Realize control object lens 1 and the distance between sample to be observed.

In the present embodiment, imaging unit 3 includes imaging sensor, converter, single-chip microcontroller, display, in which: imaging passes Sensor is for converting optical signal into electric signal and being transmitted to converter；Converter is sent for handling by imaging sensor Electric signal, and be sent to single-chip microcontroller after the electric signal is converted to digital signal；Single-chip microcontroller is sent out for encoding by converter The digital signal sent obtains image data.

In the present embodiment, imaging sensor is ccd sensor.

Alternatively, imaging sensor is cmos sensor as other embodiments.

In the present embodiment, Portable back-carrying to light optics imaging microscope include for establishing the logical of communication connection with terminal Module is interrogated, communication module is connect with single-chip microcontroller；In this way, establishing communication connection by communication module and terminal, realize monolithic The image data that machine encodes is transmitted to terminal, terminal storage and display image data.

Further, the peripheral ring of object lens 1 is equipped with resilient cushion, and the arrangement of resilient cushion breaches the limitation of real-world environment, makes More extensive usage scenario and environment can be suitable for by obtaining portable light optics imaging microscope backwards.

The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all in essence of the invention Made any modifications, equivalent replacements, and improvements etc., should all be included in the protection scope of the present invention within mind and principle.