阅读说明：本技术 一种磁微粒储存液及其制备 (A kind of magnetic particle storing liquid and its preparation ) 是由 胡亚辉 唐若愚 王唯 于 2019-07-16 设计创作，主要内容包括：本发明公开了一种磁微粒储存液及其制备,其特征在于,所述磁微粒储存液的组分包括：Tris、氯化钠、Proclin-300、吐温20、海藻糖、酪蛋白、氢氧化钠。本发明通过多种配方的组合,实现了磁微粒试剂在灵敏度、测定范围、重复性、稳定性等方面,性能得以提升。譬如,通过加入酪蛋白、海藻糖,保护了磁微粒上的结合物；加入Proclin-300作为防腐剂,抑制了细菌生长,避免试剂被污染,性能受到影响；pH调整至反应最适合的pH,利于化学发光反应稳定地进行,保证了测试结果的可重复性。综合以上因素,本发明在提升试剂本身性能的同时,保证了磁微粒储存液的长期有效。(The invention discloses a kind of magnetic particle storing liquid and its preparations, which is characterized in that the component of the magnetic particle storing liquid includes: Tris, sodium chloride, Proclin-300, polysorbas20, trehalose, casein, sodium hydroxide.The present invention by the combinations of multiple formulations, realize magnetic particle reagent sensitivity, measurement range, repeatability, in terms of, property can be promoted.For example, by the way that casein, trehalose is added, the conjugate on magnetic particle is protected；Proclin-300 is added as preservative, it is suppressed that bacterial growth avoids reagent contaminated, and performance is affected；PH is adjusted to most suitable pH is reacted, and is steadily carried out conducive to chemiluminescence reaction, be ensure that the repeatability of test result.In summary factor, the present invention ensure that the permanently effective of magnetic particle storing liquid while promoting reagent performance itself.)

1. a kind of magnetic particle storing liquid, which is characterized in that the component of the magnetic particle storing liquid includes:

Tris, sodium chloride, Proclin-300, polysorbas20, trehalose, casein, sodium hydroxide.

2. a kind of magnetic particle storing liquid as described in claim 1, which is characterized in that the content of the component is as follows:

Tris, 0.7wt% of 1wt%~1.4wt%~1.1wt% sodium chloride, 0.02vol%~0.2vol% Proclin-300,0.05vol%~0.15vol% polysorbas20, the trehalose of 0.1wt%, 0.2wt%~0.3wt% junket The sodium hydroxide of albumen, 0.02wt%, remaining content complement to 100% with water.

3. a kind of magnetic particle storing liquid as described in claim 1, which is characterized in that including following component and content:

The sodium chloride of Tris, 0.9wt% of 1.2wt%, Proclin-300,0.15vol% of 0.05vol% polysorbas20, The trehalose of 0.1wt%, the casein of 0.25wt%, 0.02wt% sodium hydroxide, remaining content complements to water 100%.

4. a kind of magnetic particle storing liquid as described in claim 1, it is characterised in that:

The color of the magnetic particle storing liquid be it is colorless and transparent, without insoluble matter.

5. a kind of magnetic particle storing liquid as described in claim 1, it is characterised in that:

The pH range of the magnetic particle storing liquid is 7.1~7.5, and the water is purified water.

6. a kind of preparation of magnetic particle storing liquid includes a kind of magnetic particle storing liquid as described in Claims 1 to 5, feature exists In, including following preparation step:

S1, measurement NaOH, casein, purified water stir to after being completely dissolved centrifugation and obtain supernatant；

S2, weigh Tris, NaCl, purified water is stirred to being completely dissolved；

S3, the pH value that solution is adjusted in solution obtained in hydrochloric acid to the step S2 is drawn with pipettor；

S4, the supernatant is added in solution obtained in the step S3, is stirred evenly；

S5, Proclin-300, polysorbas20 are measured, is stirred in solution obtained in trehalose to the step S4 to being completely dissolved；

S6, it will be mixed after the resulting solution constant volume of the step S5 with water；

S7, it will be filtered to obtain magnetic particle storing liquid by the prepared solution of step S1-S6.

7. a kind of preparation of magnetic particle storing liquid as claimed in claim 6, which is characterized in that in the step S1:

The revolving speed of the centrifugation is 4000rpm, centrifugation time 20min.

8. a kind of preparation of magnetic particle storing liquid as claimed in claim 6, which is characterized in that in the step S3:

The molar concentration of the hydrochloric acid is 5mol/L；Described draws solution obtained in hydrochloric acid to the step S2 with pipettor The middle pH value for adjusting solution is to 7.3 ± 0.2.

9. a kind of preparation of magnetic particle storing liquid as claimed in claim 6, which is characterized in that in the step S6:

Filter sizes needed for the filtering are 0.22 μm.

Technical field

The invention belongs to field of biotechnology, and in particular to a kind of magnetic particle storing liquid and its preparation.

Background technique

Magnetism particulate immuno chemistry luminescence method is to rise the new analysis method of the one kind to come into vogue in recent years.Magnetic microparticle chemiluminescence Method combines magnetic separation technique, chemiluminescence, immuno analytical method, using magnetic particle as carrier, passes through detection chemistry hair The luminous intensity of light reaction calculates the concentration of checking matter.Because of high sensitivity, high specificity, measurement range it is wide, it is easy to operate, The advantages that pollution-free, shows one's talent from a series of immuno analytical methods, becomes more and more popular.

However the storage of magnetic particle is to realize a big difficulty of Magnetism particulate immuno chemistry luminescence method, existing magnetic particle storage liquid is equal There are background value height, luminescent properties are poor, stability is poor.Because of factors such as component, pH, influenced chemiluminescence reaction into Row, leads to the decline of reagent luminescent properties itself.In addition, causing because lacking to magnetic particle and the protection of conjugate thereon Reagent stability is poor, and validity period is short.

The performance of magnetic particle tends to influence the stability of entire reagent, to impact to reagent performance.In addition, Different reagent objects detected are different, and used detection method changes therewith, and different antibody, antigen are to magnetic bead, magnetic bead There are also differences for the requirement of storing liquid.

Summary of the invention

Due to above-mentioned deficiency in the prior art, the present invention devises one for magnetic bead used in Magnetism particulate immuno chemistry luminescence method Kind storing liquid, storage, transport, use for magnetic bead in magnetic microparticle chemiluminescence reagent.

The present invention reduces background value, has correspondinglyd increase chemistry while promoting the luminescent properties of chemiluminescence reaction Furthermore the sensitivity of luminescence reagent, the range of linearity ensure that the stability of magnetic particle storage, extend having for magnetic particle storing liquid The effect phase.

The present invention is suitable for magnetic particle used in magnetic microparticle chemiluminescence reagent, is particularly directed to be pre-conjugated biotin labeling The Streptavidin magnetic particle of antibody is better than other magnetic particle storing liquids using effect of the present invention.

The present invention provides a kind of magnetic particle storing liquid to achieve the above object, and the invention adopts the following technical scheme:

The component of the magnetic particle storing liquid includes:

Tris, sodium chloride, Proclin-300, polysorbas20, trehalose, casein, sodium hydroxide.

In the technical program, by the combination of multiple formulations, realize magnetic particle storing liquid sensitivity, measurement range, Repeatability, stability etc., property can be promoted.For example, it by the way that casein, trehalose is added, protects on magnetic particle Conjugate；Proclin-300 is added as preservative, it is suppressed that bacterial growth avoids reagent contaminated, and performance is affected； PH is adjusted to most suitable pH is reacted, and is steadily carried out conducive to chemiluminescence reaction, be ensure that the repeatability of test result.It is comprehensive Factors above is closed, the present invention ensure that the permanently effective of magnetic particle storing liquid while promoting reagent performance itself.

Preferably, the content of the component is as follows:

Tris, 0.7wt% of 1wt%~1.4wt%~1.1wt% sodium chloride, 0.02vol%~0.2vol% Proclin-300,0.05vol%~0.15vol% polysorbas20, the trehalose of 0.1wt%, 0.2wt%~0.3w t% The sodium hydroxide of casein, 0.02wt%, remaining content complement to 100% with water.

Further, the content of the component is preferably as follows:

The tween of the sodium chloride of Tris, 0.9wt% of 1.2wt%, Proclin-300,0.15vol% of 0.05vol% 20, the sodium hydroxide of the trehalose of 0.1wt%, the casein of 0.25wt%, 0.02wt%, remaining content are complemented to water 100%.

Preferably, the color of the magnetic particle storing liquid is colorless and transparent, without insoluble matter.

Preferably, the pH range of the magnetic particle storing liquid is 7.1~7.5, and the water is purified water.

The present invention also provides a kind of preparations of magnetic particle storing liquid, and its step are as follows:

S1, measurement NaOH, casein, purified water stir to after being completely dissolved centrifugation and obtain supernatant；

S2, weigh Tris, NaCl, purified water is stirred to being completely dissolved；

S3, the pH value that solution is adjusted in solution obtained in hydrochloric acid to the step S2 is drawn with pipettor；

S4, the supernatant is added in solution obtained in the step S3, is stirred evenly；

S5, measure Proclin-300, polysorbas20, in solution obtained in trehalose to the step S4 stirring to complete Dissolution；

S6, it will be mixed after the resulting solution constant volume of the step S5 with water；

S7, it will be filtered to obtain magnetic particle storing liquid by the prepared solution of step S1-S6.

Preferably, in the step S1: the revolving speed of the centrifugation is 4000rpm, centrifugation time 20min.

Preferably, the molar concentration of the hydrochloric acid is 5mol/L；Described draws hydrochloric acid to the step S2 with pipettor Obtained in the pH value of solution is adjusted in solution to 7.3 ± 0.2.

In the technical program, chemiluminescence reaction needs are carried out at a certain range of PH, and pH value had both been protected to 7.3 ± 0.2 Going on smoothly for chemiluminescence reaction has been demonstrate,proved, has also avoided the performance of magnetic particle because strong acid and strong base is destroyed.

Preferably, filter sizes needed for the filtering are 0.22 μm.

The beneficial effects of the present invention are:

1, the present invention is by the combinations of multiple formulations, realize magnetic particle reagent sensitivity, measurement range, repeatability, Stability etc., property can be promoted.By the way that casein, trehalose is added, the conjugate on magnetic particle is protected；It is added Proclin-300 is as preservative, it is suppressed that bacterial growth avoids reagent contaminated, and performance is affected；PH is adjusted to reaction Most suitable pH steadily carries out conducive to chemiluminescence reaction, ensure that the repeatability of test result.In summary factor, The present invention ensure that the permanently effective of magnetic particle reagent while promoting reagent performance itself.

2, the present invention reduces background value, has correspondinglyd increase change while promoting the luminescent properties of chemiluminescence reaction The sensitivity of luminescence reagent is learned, furthermore the range of linearity ensure that the stability of magnetic particle storage, extend having for magnetic particle reagent The effect phase.

3, the present invention is suitable for magnetic particle used in magnetic microparticle chemiluminescence reagent, is particularly directed to be pre-conjugated biotin mark The Streptavidin magnetic particle for remembering antibody is better than other magnetic particle storing liquids using effect of the present invention.

Specific embodiment

The invention will now be further described with reference to specific embodiments, but these examples are merely exemplary, it is not right The scope of the present invention constitutes any restrictions.Those skilled in the art, which should be understood that, is not departing from the present invention Under the premise of principle, several improvements and modifications can also be made, these modifications and embellishments should also be considered as the scope of protection of the present invention.

The component and content of magnetic particle storing liquid are as follows: Tris, 0.7wt% of 1wt%~1.4wt%~1.1wt% chlorine Change sodium, Proclin-300,0.05vol%~0.15vol% polysorbas20 of 0.02vol%~0.2vol%, 0.1wt% Trehalose, the casein of 0.2wt%~0.3wt%, 0.02wt% sodium hydroxide, remaining content complements to 100% with water.

The present invention also provides a kind of preparation steps of magnetic particle storing liquid, and described steps are as follows:

S1, measurement NaOH, casein, purified water stir to after being completely dissolved centrifugation and obtain supernatant；

S2, weigh Tris, NaCl, ultrapure water is stirred to being completely dissolved；

S3, the pH value that solution is adjusted in solution obtained in hydrochloric acid to the step S2 is drawn with pipettor；

S4, the supernatant is added in solution obtained in the step S3, is stirred evenly；

S5, measure Proclin-300, polysorbas20, in solution obtained in trehalose to the step S4 stirring to complete Dissolution；

S6, it will be mixed after the resulting solution constant volume of the step S5 with water；

S7, it will be filtered to obtain magnetic particle storing liquid by the prepared solution of step S1-S6.