阅读说明：本技术 一种无杆动态轴向压缩色谱柱系统 (Rodless dynamic axial compression chromatographic column system ) 是由 肖立峰 孙新芬 于 2021-09-08 设计创作，主要内容包括：本发明公开了一种无杆动态轴向压缩色谱柱系统,包括色谱柱,还包括压力溶剂罐和压力溶剂泵,所述色谱柱内设有活塞,所述活塞上侧形成压力溶剂腔,活塞下侧形成填料柱床腔,所述色谱柱上端设有第一压力溶剂接头,色谱柱下端设有第二压力溶剂接头,所述压力溶剂罐的出口与所述压力溶剂泵的入口连通,所述压力溶剂泵的出口连接有进液控制阀,所述第一压力溶剂接头和第二压力溶剂接头均与所述进液控制阀连通。本发明具有结构简单、制造成本低、高度尺寸小等优点。(The invention discloses a rodless dynamic axial compression chromatographic column system which comprises a chromatographic column, a pressure solvent tank and a pressure solvent pump, wherein a piston is arranged in the chromatographic column, a pressure solvent cavity is formed on the upper side of the piston, a packed column bed cavity is formed on the lower side of the piston, a first pressure solvent joint is arranged at the upper end of the chromatographic column, a second pressure solvent joint is arranged at the lower end of the chromatographic column, an outlet of the pressure solvent tank is communicated with an inlet of the pressure solvent pump, an outlet of the pressure solvent pump is connected with a liquid inlet control valve, and the first pressure solvent joint and the second pressure solvent joint are both communicated with the liquid inlet control valve. The invention has the advantages of simple structure, low manufacturing cost, small height and size and the like.)

1. A rodless dynamic axial compression chromatography column system comprising a chromatography column (1), characterized by: still include pressure solvent jar (2) and pressure solvent pump (3), be equipped with piston (4) in chromatographic column (1), piston (4) upside forms pressure solvent chamber (11), and piston (4) downside forms packed column bed chamber (12), chromatographic column (1) upper end is equipped with first pressure solvent and connects (13), and chromatographic column (1) lower extreme is equipped with second pressure solvent and connects (14), the export of pressure solvent jar (2) with the entry intercommunication of pressure solvent pump (3), the exit linkage of pressure solvent pump (3) has into liquid control valve (31), first pressure solvent connect (13) and second pressure solvent connect (14) all with it communicates to advance liquid control valve (31).

2. The rodless dynamic axial compression chromatography column system of claim 1, wherein: and a pressure sensor (32) is arranged between the pressure solvent pump (3) and the liquid inlet control valve (31).

3. The rodless dynamic axial compression chromatography column system of claim 1, wherein: the pressure solvent tank (2) is connected with a backflow control valve (21), and the first pressure solvent connector (13) and the second pressure solvent connector (14) are communicated with the backflow control valve (21).

4. The rodless dynamic axial compression chromatography column system of claim 3, wherein: the liquid inlet control valve (31) and the backflow control valve (21) are three-way valves, the outlet of the pressure solvent pump (3) is communicated with the inlet of the liquid inlet control valve (31), one outlet of the liquid inlet control valve (31) is communicated with the first pressure solvent joint (13), the other outlet of the liquid inlet control valve is communicated with the second pressure solvent joint (14), one inlet of the backflow control valve (21) is communicated with the first pressure solvent joint (13), the other inlet of the backflow control valve (21) is communicated with the second pressure solvent joint (14), and the outlet of the backflow control valve (21) is communicated with the pressure solvent tank (2).

5. The rodless dynamic axial compression chromatography column system of claim 3, wherein: the liquid inlet control valve (31) and the backflow control valve (21) are both pneumatic ball valves.

6. The rodless dynamic axial compression chromatography column system of any of claims 1-5, wherein: still include homogenate jar (5), sample jar (6) and fraction collection tank (7), chromatographic column (1) upper end is equipped with first sample and connects (15), chromatographic column (1) lower extreme is equipped with homogenate and connects (16) and fraction recovery joint (17), homogenate jar (5) through homogenate pump (51) with homogenate connects (16) intercommunication, sample jar (6) through a sample pump (61) with first sample connects (15) intercommunication, fraction recovery joint (17) with fraction collection tank (7) intercommunication.

7. The rodless dynamic axial compression chromatography column system of claim 6, wherein: and a second sample joint (41) for communicating the pressure solvent cavity (11) with the packed bed cavity (12) is arranged on the piston (4), and the first sample joint (15) is communicated with the second sample joint (41).

8. The rodless dynamic axial compression chromatography column system of claim 7, wherein: the first sample connector (15) is communicated with the second sample connector (41) through a telescopic pipeline (42).

9. The rodless dynamic axial compression chromatography column system of claim 6, wherein: chromatographic column (1) includes column tube (18) and can dismantle column tube lower cover (19) of being connected with column tube (18) lower extreme, second pressure solvent connect (14) with homogenate connects (16) to be located column tube (18) lower extreme, fraction recovery connects (17) to be located on column tube lower cover (19), homogenate jar (5) are located the below of chromatographic column (1).

Technical Field

The invention relates to a chromatographic column, in particular to a rodless dynamic axial compression chromatographic column system.

Background

Chromatographic separation techniques are widely used to separate chemical and biological substances, and liquid chromatographic separation plays an important role in the pharmaceutical and biological industries for the preparation, purification, and analysis of proteins and nucleic acids, etc. The technical scheme includes that a piston (or a piston assembly) is arranged in a column tube and is driven by a piston rod (or a rod-shaped component) to move up and down in the column tube, the whole structure is complex, the manufacturing cost is high, the height size of the system is large, the space occupied in the vertical direction is large, and meanwhile, when the packing is disassembled, more manual participation or auxiliary equipment is needed, so that the convenience is not high, and secondary pollution of the packing is easily caused.

Disclosure of Invention

The technical problem to be solved by the invention is to overcome the defects of the prior art and provide a rodless dynamic axial compression chromatographic column system with simple structure, low manufacturing cost and small height size.

In order to solve the technical problems, the invention adopts the following technical scheme:

the utility model provides a no pole developments axial compression chromatographic column system, includes the chromatographic column, still includes pressure solvent jar and pressure solvent pump, be equipped with the piston in the chromatographic column, the piston upside forms the pressure solvent chamber, and the piston downside forms packed column bed chamber, the chromatographic column upper end is equipped with first pressure solvent and connects, and the chromatographic column lower extreme is equipped with second pressure solvent and connects, the export of pressure solvent jar with the entry intercommunication of pressure solvent pump, the exit linkage of pressure solvent pump has into liquid control valve, first pressure solvent connect and second pressure solvent connect all with advance liquid control valve intercommunication.

As a further improvement of the above technical solution:

and a pressure sensor is arranged between the pressure solvent pump and the liquid inlet control valve.

The pressure solvent tank is connected with a reflux control valve, and the first pressure solvent joint and the second pressure solvent joint are communicated with the reflux control valve.

The liquid inlet control valve and the backflow control valve are three-way valves, an outlet of the pressure solvent pump is communicated with an inlet of the liquid inlet control valve, an outlet of the liquid inlet control valve is communicated with the first pressure solvent joint, the other outlet of the liquid inlet control valve is communicated with the second pressure solvent joint, an inlet of the backflow control valve is communicated with the first pressure solvent joint, the other inlet of the backflow control valve is communicated with the second pressure solvent joint, and an outlet of the backflow control valve is communicated with the pressure solvent tank.

And the liquid inlet control valve and the backflow control valve are pneumatic ball valves.

Still include homogenate jar, sample jar and fraction collection tank, the chromatographic column upper end is equipped with first sample and connects, the chromatographic column lower extreme is equipped with homogenate liquid and connects and fraction recovery joint, homogenate jar through an homogenate pump with homogenate liquid connects the intercommunication, the sample jar through a sample pump with first sample connects the intercommunication, fraction recovery joint with fraction collection tank intercommunication.

And a second sample joint for communicating the pressure solvent cavity and the packed column bed cavity is arranged on the piston, and the first sample joint is communicated with the second sample joint.

The first sample joint is communicated with the second sample joint through a telescopic pipeline.

The chromatographic column comprises a column tube and a column tube lower cover detachably connected with the lower end of the column tube, the second pressure solvent joint and the homogenate liquid joint are arranged at the lower end of the column tube, the fraction recovery joint is arranged on the column tube lower cover, and the homogenate tank is arranged below the chromatographic column.

Compared with the prior art, the invention has the advantages that: the invention discloses a rodless dynamic axial compression chromatographic column system, which comprises a pressure solvent tank and a pressure solvent pump, wherein the upper end and the lower end of a chromatographic column are respectively provided with a first pressure solvent joint and a second pressure solvent joint; on the contrary, when the pressure solvent enters the packed column bed cavity through the second pressure solvent joint, the pressure on the lower side of the piston is greater than the pressure on the upper side under the action of the pressure solvent, the piston can move upwards, the piston does not need to be provided with a piston rod, the structure is simplified, the manufacturing cost is reduced, and the height size of the chromatographic column is greatly reduced.

Drawings

FIG. 1 is a schematic representation of the rodless dynamic axial compression chromatography column system of the present invention.

The reference numerals in the figures denote: 1. a chromatographic column; 11. a pressurized solvent chamber; 12. a packed column bed chamber; 13. a first pressure solvent joint; 14. a second pressure solvent joint; 15. a first sample joint; 16. a homogenate joint; 17. a distillate recovery sub; 18. a column tube; 19. a lower cover of the column tube; 2. a pressurized solvent tank; 21. a reflux control valve; 3. a pressurized solvent pump; 31. a liquid inlet control valve; 32. a pressure sensor; 4. a piston; 41. a second sample adaptor; 42. a telescopic pipeline; 5. a homogenizing tank; 51. a homogenizing pump; 6. a sample tank; 61. a sample pump; 7. and a fraction collection tank.

Detailed Description

The invention is described in further detail below with reference to the figures and specific examples of the specification.

Fig. 1 shows an embodiment of a rodless dynamic axial compression chromatographic column system according to the present invention, which includes a chromatographic column 1, a pressure solvent tank 2 and a pressure solvent pump 3, wherein a piston 4 is disposed in the chromatographic column 1, a pressure solvent chamber 11 is formed on the upper side of the piston 4, a packed bed chamber 12 is formed on the lower side of the piston 4, a first pressure solvent joint 13 is disposed at the upper end of the chromatographic column 1, a second pressure solvent joint 14 is disposed at the lower end of the chromatographic column 1, an outlet of the pressure solvent tank 2 is communicated with an inlet of the pressure solvent pump 3, an outlet of the pressure solvent pump 3 is connected with a liquid inlet control valve 31, and both the first pressure solvent joint 13 and the second pressure solvent joint 14 are communicated with the liquid inlet control valve 31.

The rodless dynamic axial compression chromatographic column system comprises a chromatographic column 1, a pressure solvent tank 2 and a pressure solvent pump 3, wherein a first pressure solvent joint 13 and a second pressure solvent joint 14 are respectively arranged at the upper end and the lower end of the chromatographic column 1, a pressure solvent in the pressure solvent tank 2 is output through the pressure solvent pump 3, the pressure solvent is controlled to enter the first pressure solvent joint 13 or the second pressure solvent joint 14 through a liquid inlet control valve 31, when the pressure solvent enters a pressure solvent cavity 11 through the first pressure solvent joint 13, the pressure on the upper side of a piston 4 is greater than the pressure on the lower side of the piston 4 under the action of the pressure solvent, and the piston 4 can move downwards; on the contrary, when the pressure solvent enters the packed column bed cavity 12 through the second pressure solvent joint 14, the pressure on the lower side of the piston 4 is greater than the pressure on the upper side under the action of the pressure solvent, the piston 4 can move upwards, the piston 4 does not need to be provided with a piston rod, the structure is simplified, the manufacturing cost is reduced, and the height size of the chromatographic column 1 is greatly reduced. Among these, in order to prevent the packing in the bed from being contaminated by the pressure solvent in the event of failure of the piston 4, the pressure solvent is preferably the reagent used in the initial homogenization of the packing.

Further, in the present embodiment, a pressure sensor 32 is provided between the pressure solvent pump 3 and the liquid inlet control valve 31. The pressure of the pressurized solvent output by the pressurized solvent pump 3 can be monitored in real time by the pressure sensor 32.

Further, in the present embodiment, a reflux control valve 21 is connected to the pressure solvent tank 2, and the first pressure solvent connector 13 and the second pressure solvent connector 14 are both communicated with the reflux control valve 21. When the piston 4 needs to move downwards, the pressure solvent in the pressure solvent tank 2 enters the pressure solvent cavity 11, if the pressure solvent exists in the packed column bed cavity 12, the pressure solvent in the packed column bed cavity 12 flows back to the pressure solvent tank 2 through the backflow control valve 21 along with the downward movement of the piston 4, and the recovery and the cyclic utilization of the pressure solvent are realized; on the contrary, when the piston 4 needs to move upwards, the pressure solvent in the pressure solvent tank 2 enters the packed bed chamber 12, and if the pressure solvent is in the pressure solvent chamber 11, the pressure solvent in the pressure solvent chamber 11 flows back to the pressure solvent tank 2 through the backflow control valve 21 along with the upward movement of the piston 4, so that the recovery and the cyclic utilization of the pressure solvent are realized.

Further, in this embodiment, the liquid inlet control valve 31 and the return flow control valve 21 are both three-way valves, the outlet of the pressure solvent pump 3 is communicated with the inlet of the liquid inlet control valve 31, one outlet of the liquid inlet control valve 31 is communicated with the first pressure solvent joint 13, the other outlet is communicated with the second pressure solvent joint 14, and the pressure solvent output by the pressure solvent pump 3 is controlled to enter the first pressure solvent joint 13 or the second pressure solvent joint 14 by switching of the three-way valves; an import and the first pressure solvent of backflow control valve 21 connect 13 the intercommunication, another import and the second pressure solvent connects 14 the intercommunication, and the export and the pressure solvent jar 2 intercommunication of backflow control valve 21 control first pressure solvent through the switching of three-way valve connect 13 or the second pressure solvent connects 14 to flow back to in the pressure solvent jar 2, are favorable to simplifying the connecting line of system, and control is convenient.

In a preferred embodiment, both the inlet control valve 31 and the return control valve 21 are pneumatic ball valves.

In this embodiment, no pole developments axial compression chromatographic column system still includes homogenate jar 5, sample jar 6 and fraction collection tank 7, and 1 upper end of chromatographic column is equipped with first sample joint 15, and 1 lower extreme of chromatographic column is equipped with homogenate joint 16 and fraction recovery joint 17, and homogenate jar 5 communicates with homogenate joint 16 through a homogenate pump 51, and sample jar 6 communicates with first sample joint 15 through a sample pump 61, and fraction recovery joint 17 communicates with fraction collection tank 7. In a preferred embodiment, the piston 4 is provided with a second sample joint 41 for communicating the pressure solvent cavity 11 and the packed bed cavity 12, and the first sample joint 15 is communicated with the second sample joint 41, so that the sample can enter the packed bed cavity 12 at the lower side through the piston 4; the first sample connector 15 is communicated with the second sample connector 41 through an expansion pipeline 42 (such as a metal hose), and when the second sample connector 41 moves up and down along with the piston 4, the expansion pipeline 42 expands and contracts, so that the first sample connector 15 and the second sample connector 41 are always kept communicated, and the structure is simple and reliable.

As a preferred embodiment, the column 1 comprises a column tube 18 and a lower column tube cover 19 detachably connected to the lower end of the column tube 18, the second pressure solvent connector 14 and the homogenate connector 16 are provided at the lower end of the column tube 18, the fraction recovery connector 17 is provided on the lower column tube cover 19, and the homogenate tank 5 is provided below the column 1. Open pillar pipe lower cover 19 and homogenate jar 5, under the effect of pressure solvent, in the homogenate jar 5 of the downside is impressed with the filler column bed to the decline of piston 4, simple structure, convenient to use have avoided the artifical secondary pollution problem of unloading and leading to.

The working principle of the rodless dynamic axial compression chromatographic column system is as follows:

1) the filler is stirred uniformly in the homogenizing tank 5 and then pumped into the packed column bed chamber 12 at the lower end of the column 1 by a homogenizing pump 51 (e.g., a pneumatic diaphragm pump).

2) And column packing: the piston 4 moves down: liquid is fed into the first pressure solvent joint 13 through the liquid inlet control valve 31, the pressure solvent is input into the pressure solvent cavity 11 at the upper end of the column tube 18 through the pressure solvent pump 3, the piston 4 is pushed to move downwards, the flowing phase in the homogenate liquid in the packed column bed cavity 12 is pressed out through the fraction collecting joint 17 and then is discharged, and the homogenate liquid in the packed column bed cavity 12 reaches the set pressure.

3) After the chromatographic column 1 is filled, the sample to be processed in the sample tank 6 is uniformly input into the column bed through the first sample joint 15, the telescopic pipeline 42 and the second sample joint 41 in sequence by using the sample pump 61 for purification treatment.

4) The processed sample enters the fraction collection tank 7 through the fraction recovery joint 17 to be collected.

5) And dismantling the column: the piston 4 moves down: and (3) removing the lower cover 19 of the column tube, opening the homogenization tank 5, switching to the first pressure solvent joint 13 through the liquid inlet control valve 31 to feed liquid, inputting the pressure solvent into the pressure solvent cavity 11 through the pressure solvent pump 3, pushing the piston 4 to move downwards, and extruding the packed column bed from the lower end of the column tube 18 into the homogenization tank 5.

6) And the piston 4 moves upwards: the column tube lower cover 19 is assembled, liquid is fed into the second pressure solvent joint 14 through the liquid inlet control valve 31, the backflow control valve 21 is opened to enable the first pressure solvent joint 13 to be communicated with the backflow port of the pressure solvent tank 2, the pressure solvent is input into the packed column bed cavity 12 through the pressure solvent pump 3, the piston 4 is pushed to move upwards, the pressure solvent in the pressure solvent cavity 11 is recovered into the pressure solvent tank 2 through the backflow control valve 21, after the piston 4 moves upwards to the set position, the pressure solvent pump 3 stops working, the backflow control valve 21 is switched to enable the second pressure solvent joint 14 to be communicated with the backflow port of the pressure solvent tank 2, and the pressure solvent in the packed column bed cavity 12 flows back into the pressure solvent tank 2.

Although the present invention has been described with reference to the preferred embodiments, it is not intended to be limited thereto. Those skilled in the art can make numerous possible variations and modifications to the present invention, or modify equivalent embodiments to equivalent variations, without departing from the scope of the invention, using the teachings disclosed above. Therefore, any simple modification, equivalent change and modification made to the above embodiments according to the technical spirit of the present invention should fall within the protection scope of the technical scheme of the present invention, unless the technical spirit of the present invention departs from the content of the technical scheme of the present invention.