阅读说明：本技术 一种从杜仲叶中提取总黄酮的制备方法 (Preparation method for extracting total flavonoids from eucommia leaves ) 是由 杨成东 于 2018-05-29 设计创作，主要内容包括：本发明公开了一种从杜仲叶中提取总黄酮的制备方法,其特征在于以下步骤：1)取干燥杜仲叶叶,研磨制成粗粉；2)取粗粉,加入无水乙醇80ml,超声3次,功率120~240w,每次30min,合并提取液,回收乙醇,得到杜仲叶浸膏；3)提取：取杜仲叶浸膏加入适量蒸馏水,搅匀,加入到层析柱中调节流速为5ml/min,用蒸馏水洗去杂质,以10~50%乙醇为流动相解吸附；4)回收乙醇,得总黄酮提取物,真空干燥即得总黄酮。本发明制备的总黄酮,产品纯度高,品质好,工艺方法简便易行,适用于工业化生产。(The invention discloses a preparation method for extracting total flavonoids from eucommia leaves, which is characterized by comprising the following steps: 1) grinding dry folium Eucommiae into coarse powder; 2) taking the coarse powder, adding 80ml of absolute ethyl alcohol, performing ultrasonic treatment for 3 times with the power of 120-240 w for 30min each time, combining extracting solutions, and recovering the ethyl alcohol to obtain eucommia leaf extract; 3) extraction: adding a proper amount of distilled water into the eucommia ulmoides leaf extract, uniformly stirring, adding the mixture into a chromatographic column, adjusting the flow rate to be 5ml/min, washing the mixture with distilled water to remove impurities, and desorbing the mixture by using 10-50% ethanol as a mobile phase; 4) recovering ethanol to obtain total flavone extract, and vacuum drying to obtain total flavone. The total flavone prepared by the method has high product purity, good quality, simple and easy process method, and is suitable for industrial production.)

1. a preparation method for extracting total flavonoids from eucommia leaves is characterized by comprising the following steps:

1) Grinding dried folium Eucommiae into coarse powder;

2) Taking the coarse powder, adding 80ml of absolute ethyl alcohol, performing ultrasonic treatment for 3 times with the power of 120-240 w for 30min each time, combining extracting solutions, and recovering the ethyl alcohol to obtain eucommia leaf extract;

3) Extraction: adding a proper amount of distilled water into the eucommia ulmoides leaf extract, uniformly stirring, adding the mixture into a chromatographic column, adjusting the flow rate to be 5ml/min, washing the mixture with distilled water to remove impurities, and desorbing the mixture by using 10-50% ethanol as a mobile phase;

4) recovering ethanol to obtain total flavone extract, and vacuum drying to obtain total flavone.

2. The method for extracting total flavonoids from eucommia ulmoides leaves according to claim 1, wherein the ultrasonic power in the step 2) is 60-200W.

3. The preparation method for extracting total flavonoids from folium cortex eucommiae as claimed in claim 1, wherein the chromatographic column in step 3) is prepared by pretreating D101 type macroporous resin and polyamide, and the content of ethanol desorbed by a mobile phase is 30-60%.

Technical Field

the invention belongs to the technical field of medicines, and particularly relates to a preparation method of total flavonoids.

Background

Eucommia ulmoides is the dried bark of Eucommia ulmoides (Eucommia ulmoides Oliver) belonging to family Eucommiaceae, and is a famous and precious tonic herb unique to China. Research results show that the eucommia ulmoides has very obvious effects on promoting body functions, resisting aging, resisting cancer and the like, particularly has bidirectional regulation effect on blood pressure, is incomparable with any chemical medicine, belongs to the first-class health-care medicine for the elderly, and the extract of the eucommia ulmoides has been widely used as a health-care food component with homology of medicine and food in China. Eucommia bark mainly contains organic compounds such as lignans, iridoids, flavonoids, polysaccharide, amino acid, gutta percha and the like, and inorganic elements such as calcium, iron and the like. The flavonoid compound contained in the eucommia ulmoides oliv extract is one of main effective components of the eucommia ulmoides oliv, and has the activities of resisting oxidation and removing free radicals.

Flavonoids (flavanones) are a class of compounds that occur in nature and have the structure of 2-phenyl chromones (flavanones). They have a keto carbonyl group in their molecule, the first oxygen atom is basic and can form salts with strong acids, and their hydroxy derivatives are often yellow, so they are also called flavonones or flavones. Flavonoids are usually combined with sugars in plants to form glycosides, and a small part exists in the form of free form (aglycones). Most plants contain flavonoid compounds, which play important roles in plant growth, development, flowering, fruiting, antibiosis, disease prevention and the like.

In recent years, an ultrasonic extraction method is widely applied to extraction of effective components in natural plants as an excellent extraction method, and compared with a Soxhlet extraction method, a reflux extraction method and an ultrasonic extraction method for extracting total flavonoids, the result shows that the ultrasonic extraction method is higher in extraction rate than the Soxhlet extraction method and the reflux extraction method, and is shorter in time, so that the ultrasonic extraction method is more effective and simpler in method for extracting the total flavonoids.

Disclosure of Invention

The invention aims to provide a method for extracting total flavonoids, and the total flavonoids have wide medicinal and health-care values.

the invention is completed by the following technical scheme, and the specific content comprises the following steps: method for extracting total flavone

1) Grinding dried folium Eucommiae into coarse powder;

2) Taking the coarse powder, adding 80ml of absolute ethyl alcohol, performing ultrasonic treatment for 3 times with the power of 120-240 w for 30min each time, combining extracting solutions, and recovering the ethyl alcohol to obtain eucommia leaf extract;

3) Extraction: adding a proper amount of distilled water into the eucommia ulmoides leaf extract, uniformly stirring, adding the mixture into a chromatographic column, adjusting the flow rate to be 5ml/min, washing the mixture with distilled water to remove impurities, and desorbing the mixture by using 10-50% ethanol as a mobile phase;

4) recovering ethanol to obtain total flavone extract, and vacuum drying to obtain total flavone.

The power of the ultrasonic wave in the step 2) is 120-240W.

the chromatographic column in the step 3) is prepared by pretreating D101 type macroporous resin and polyamide, and the content of the mobile phase desorption ethanol is 10-50%.

the invention has the advantages that

the invention adopts ultrasonic extraction-D101 type macroporous resin and polyamide column chromatography to extract the total flavone in the eucommia leaves, the sample capacity of the column chromatography is large, the extraction efficiency is high, and the method is simple and easy to implement.

The present invention will be further described with reference to specific embodiments, but the scope of the invention as claimed is not limited to the following embodiments.

The specific implementation mode is as follows: