阅读说明：本技术 一种茯苓皮提取物制备方法及应用 (preparation method and application of poria peel extract ) 是由 杨成东 于 2018-05-30 设计创作，主要内容包括：本发明公开了一种茯苓皮提取物制备方法及应用,所述茯苓皮提取物制备方法包括：a、将茯苓皮用乙醇水溶液提取,取滤液备用；b、将所得药渣加乙醇重复提取；c、合并滤液,于恒温水浴蒸干得茯苓皮提取物。本发明所述茯苓皮提取物主要为三萜类和多糖类等,性平,味甘、淡,归心、肺、脾、肾经,功专利水消肿。(The invention discloses a preparation method and application of a poria peel extract, wherein the preparation method of the poria peel extract comprises the following steps: a. extracting exodermis Poria with ethanol water solution, and collecting filtrate; b. extracting the residue with ethanol repeatedly; c. mixing filtrates, and evaporating to dryness in constant temperature water bath to obtain Poria peel extract. The poria peel extract provided by the invention mainly comprises triterpenes, polysaccharides and the like, is neutral in nature, sweet and light in taste, and has the effects of nourishing heart, lung, spleen and kidney channels, and removing edema.)

1. The preparation method and application of the poria peel extract are characterized in that the preparation method of the poria peel extract comprises the following steps: a. extracting exodermis Poria with ethanol water solution, and collecting filtrate; b. extracting the residue with ethanol repeatedly; c. mixing filtrates, and evaporating to dryness in constant temperature water bath to obtain Poria peel extract.

2. The method of claim 1, wherein the concentration of the aqueous ethanol solution in step a is 95% and the amount is 3 times of the volume of the poria peel, and the ultrasonic extraction is performed for 30 min.

3. The process of claim 1, wherein the concentration of the ethanol aqueous solution in step b is 95%, and the extraction is repeated for 2 times.

4. The method of claim 1, wherein the 3 filtrates are combined and evaporated to dryness in a thermostatic water bath at 60 ℃.

Technical Field

The invention relates to the technical field of biological extraction, and particularly relates to a preparation method and application of a poria peel extract.

Background

Poria is a dry fungal sclerotium of polyporaceae family polyporaceae genus of polyporaceae, mainly produced in Yunnan, Anhui and Hubei provinces, its sex is flat, the taste is sweet, light, it has effects of diuresis, eliminating dampness, invigorating spleen, regulating stomach and calming the heart and tranquilization, mainly used for treating dysuresia, edema, spleen and stomach qi deficiency, anorexia, loose stool, tired and weak power and diseases such as the restlessness of heart prescription, pavor and insomnia, it is the raw materials of many Chinese traditional medicines, there is "ten medicine nine Poria" said. The outer skin of the medicine is poria peel, has mild nature, sweet and light taste, and enters heart, lung, spleen and kidney channels, and the medicine has the effect of relieving swelling. The chemical components separated from Poria mainly include triterpenes, polysaccharides and steroids, while the exodermis Poria mainly includes triterpenes and polysaccharides. In the literature, poria peel is a commonly used diuretic detumescence drug in China, but is discarded as leftover material in Japan, which causes huge loss of resources. With the increasing reports of adverse reactions of western medicines to diuretic, it is becoming more and more important to find a traditional Chinese medicine diuretic with good effect. For a long time, the research on the tuckahoe peel mainly focuses on the activities of resisting tumors, inflammation and bacteria, but neglects the traditional diuretic activity. The research and the diuretic activity of the poria peel are preliminarily researched, and an experimental basis is provided for further clarifying diuretic active sites and chemical components of the poria peel.

Disclosure of Invention

Based on the above purposes, the invention provides a preparation method of a poria peel extract and application of the poria peel extract in the aspect of diuretic activity.

the invention relates to a poria peel extract, and the preparation method comprises the following steps:

a. Adding 3 times volume of 95% ethanol into 1.5 kg of exodermis Poria, and ultrasonically extracting for 30min to obtain filtrate for later use;

b. Extracting the residue with 95% ethanol for 2 times;

c. Mixing the filtrates, evaporating to dryness in 60 deg.C constant temperature water bath to obtain 279 g of exodermis Poria ethanol extract with extraction rate of 18.6%, and storing in refrigerator for use.

The invention provides an application of a poria peel extract in the aspect of diuretic activity.

SD rats were 40 and randomly divided into 5 groups of 8 rats each. One group was a normal control group, and one group was a positive furosemide control group. Before the experiment, water is not forbidden for 12 hours. The stomach of each group is irrigated with 50 mL/kg of physiological saline, and the retention of sodium and water in the rat body is caused. After 30min, the normal control group was perfused with 10 mg/kg tap water, the positive control group was administered with 20 mg/kg furosemide solution, and the other 3 groups were administered with 150, 300 and 600 mg/kg ethanol extracts of Poria cocos (equivalent to crude drug amounts of 0.85, 1.7 and 3.4 g/kg, respectively). Immediately after dosing, rats were placed in metabolic cages, 1 per cage, graduated tubes were placed under the metabolic cages, urine was recorded 1 time per hour and collected for 6 h continuously. The pH value of the urine and the contents of Na +, K + and Cl-are measured.

Within 6 h after the administration, the urine volume of the rats is increased remarkably compared with the water control group (P < 0.01),

Respectively increased by 29%, 21% and 42%, and has diuretic effect uniformly distributed within 1-6 hr

And (4) acting. The diuretic effect of furosemide is very obvious (P < 0.01). Urine Na of each group of poria peel ethanol extract

The ratio of +/-K + is obviously increased (P is less than 0.05, and P is less than 0.01). Poria peel can increase urine

The pH value. The furosemide increases Na + and Cl-discharge (P < 0.01) and increases Na +/K + value (P)

Less than 0.01) and has no obvious influence on the pH value of urine.

Research results show that the poria peel ethanol extract has a remarkable diuretic effect. Furosemide is a loop diuretic, influences dilution and concentration of urine by inhibiting a loop ascending and branching thick segment Na + -K + -2 Cl-co-transporter, generates a rapid and strong diuretic effect, expands systemic arterioles while inducing diuresis, reduces peripheral resistance, increases renal blood flow without reducing glomerular filtration rate, and is the most powerful diuretic currently. The ethanol extract of the tuckahoe peel has mild and lasting diuretic effect and is distributed for 1 to 6 hours.

The poria peel ethanol extract can increase the discharge of electrolyte Na + and reduce the discharge of K + while promoting diuresis, so that the ratio of Na +/K + in urine is increased, which indicates that the poria peel ethanol extract can discharge Na + and promote diuresis, the mechanism of the poria peel ethanol extract can inhibit the Na + -K + exchange of renal distal convoluted tubule and collecting duct, and the potassium-retaining and sodium-discharging effect similar to that of the spironolactone which is a low-efficiency diuretic is generated.

Detailed Description

the present invention will be further described with reference to the following examples.