阅读说明：本技术 一种用于外伤急救止血材料的制备方法 (Preparation method of hemostatic material for trauma emergency treatment ) 是由 吕文亮 邢霞 刘聚伟 刘杰 于 2021-09-02 设计创作，主要内容包括：本发明公开了一种用于外伤急救止血材料的制备方法,包括以下步骤：将明胶在40-60℃温度下将其溶解于去离子水,配制成明胶溶液；在明胶溶液中加入三维多孔双交联聚苯胺/石墨烯复合干凝胶,磁力搅拌使干凝胶分散均匀,添加中药成分混合液,然后倒入模具中,常温下静置并排出气泡,然后放入零下40℃-零下25℃的低温下预冻,然后在真空冷冻干燥机中冷冻干燥,得到混合干凝胶,将混合干凝胶从模具中取出,并在上下两面分别覆盖一层医用纱布,紧密贴合后,即为用于外伤急救止血材料。该材料可以对各种出血情况及时止血,一般止血所需时间低于5分钟。(The invention discloses a preparation method of a hemostatic material for trauma emergency treatment, which comprises the following steps: dissolving gelatin in deionized water at 40-60 deg.C to obtain gelatin solution; adding three-dimensional porous double-crosslinked polyaniline/graphene composite xerogel into gelatin solution, uniformly dispersing the xerogel by magnetic stirring, adding a traditional Chinese medicine component mixed solution, pouring into a mold, standing at normal temperature and discharging air bubbles, pre-freezing at the low temperature of minus 40-minus 25 ℃, freeze-drying in a vacuum freeze dryer to obtain mixed xerogel, taking the mixed xerogel out of the mold, respectively covering a layer of medical gauze on the upper surface and the lower surface, and tightly attaching to obtain the trauma emergency hemostatic material. The material can timely stop bleeding for various bleeding conditions, and the time for stopping bleeding is generally less than 5 minutes.)

1. A preparation method of a hemostatic material for trauma emergency treatment is characterized by comprising the following steps:

(1) dissolving gelatin in deionized water at 40-60 deg.C to obtain gelatin solution with mass concentration of 15-25%;

(2) adding three-dimensional porous double-crosslinked polyaniline/graphene composite xerogel into gelatin solution, and magnetically stirring at 300-400rpm for 20-30min to uniformly disperse the xerogel to obtain mixed solution;

(3) adding the mixed solution of the traditional Chinese medicine components into the mixed solution, pouring the mixed solution into a mold, keeping the liquid layer at the thickness of 5-8mm for 45-60min at normal temperature, discharging air bubbles, pre-freezing the mixed solution at the low temperature of minus 40-minus 25 ℃ for 1-1.5h, and freeze-drying the mixed solution in a vacuum freeze dryer to obtain mixed xerogel;

(4) and taking the mixed xerogel out of the die, respectively covering a layer of medical gauze on the upper surface and the lower surface, and tightly attaching to obtain the trauma emergency hemostatic material.

2. The preparation method of the trauma emergency hemostatic material according to claim 1, wherein in the step (2), the addition amount of the three-dimensional porous double-crosslinked polyaniline/graphene composite xerogel is 0.1-0.3% of the mass of the gelatin solution.

3. The method for preparing a hemostatic material for trauma emergency treatment according to claim 1, wherein in the step (3), the amount of the mixed solution of the Chinese medicinal materials is 1.8-3.5% by mass of the gelatin solution.

4. The preparation method of the trauma emergency hemostatic material according to claim 1, wherein the traditional Chinese medicine component mixed solution comprises the following components by weight percent: 2-5% of vaseline, 1-3% of sodium dodecyl sulfate, 3-6% of semisynthetic fatty glyceride, 0.1-0.3% of borneol, 1.8-2.5% of glycerol, 0.5-0.8% of pearl powder, 8-12% of distilled water and the balance of traditional Chinese medicine extract.

5. The method for preparing a hemostatic material for trauma emergency treatment according to claim 4, wherein the Chinese medicinal extractive solution is prepared by extracting the following Chinese medicinal herbs with water: cortex Phellodendri, radix et rhizoma Rhei, rhizoma Polygoni Cuspidati, radix Saposhnikoviae, flos Sophorae Immaturus, radix Angelicae sinensis, Glycyrrhrizae radix, Bulbus Lilii, artificial Moschus, rhizoma Gymnadeniae and lignum Dalbergiae Odoriferae.

6. The preparation method of the trauma emergency hemostasis material of claim 5, wherein the traditional Chinese medicine extract comprises the following traditional Chinese medicine components in parts by weight: 5-20 parts of phellodendron, 5-20 parts of rhubarb, 2-10 parts of giant knotweed, 3-15 parts of divaricate saposhnikovia root, 3-15 parts of sophora flower, 8-25 parts of angelica, 15-25 parts of liquorice, 2-10 parts of lily, 2-10 parts of artificial musk, 5-20 parts of gymnadenia conopsea and 3-15 parts of dalbergia wood.

7. The preparation method of the trauma emergency hemostasis material of claim 5, wherein the traditional Chinese medicine extract comprises the following traditional Chinese medicine components in parts by weight: 8-15 parts of phellodendron, 8-15 parts of rheum officinale, 5-8 parts of polygonum cuspidatum, 6-12 parts of divaricate saposhnikovia root, 6-12 parts of sophora flower, 10-18 parts of angelica sinensis, 15-25 parts of liquorice, 5-8 parts of lily, 5-8 parts of artificial musk, 10-18 parts of gymnadenia conopsea and 6-12 parts of rosewood heart wood.

8. The preparation method of the trauma emergency hemostatic material according to claim 4, wherein the preparation method of the mixed solution of the traditional Chinese medicine components comprises the following steps:

A. soaking cortex Phellodendri, radix et rhizoma Rhei, rhizoma Polygoni Cuspidati, radix Saposhnikoviae, flos Sophorae Immaturus, radix Angelicae sinensis, Glycyrrhrizae radix, Bulbus Lilii, rhizoma Gymnadeniae and lignum Dalbergiae Odoriferae in water, reflux-extracting twice, collecting the two water extractive solutions, mixing, and filtering; concentrating the filtrate to relative specific gravity of 1.05-1.10 to obtain Chinese medicinal ointment;

B. mixing vaseline and semisynthetic fatty glyceride, heating to dissolve, and keeping the temperature at 75-78 deg.C to obtain oil phase;

C. mixing sodium laurylsulfate, glycerol, distilled water and Chinese medicinal paste, heating, adding Borneolum Syntheticum and Margarita powder, stirring, and keeping the temperature at 60-65 deg.C to obtain water phase;

D. slowly adding the oil phase into the water phase, stirring, and cooling to 40-45 deg.C.

9. The method for preparing a hemostatic material for trauma emergency treatment according to claim 8, wherein in step C, the particle size of the pearl powder and borneol is 250 mesh.

Technical Field

The invention relates to the technical field of hemostatic external drugs, in particular to a preparation method of a hemostatic material for trauma emergency treatment.

Background

Major hemorrhage refers to a phenomenon of massive hemorrhage caused by rupture of arteries, injury of internal organs, or the like. Major bleeding is a common problem in natural disasters, traffic accidents, and emergencies.

The normal hemostasis of the body mainly depends on the structure and function of the intact blood vessel wall, the effective quality and quantity of blood platelets and the activity of normal blood coagulation factors, wherein the effects of the blood platelets with negative charges and the blood coagulation factors are the main. The hemostatic sponge is a hemostatic material which is fine, porous and positively charged, and can immediately adhere and aggregate platelets when contacting human blood, so that the blood forms thrombus and blocks wounds, various factors related to blood coagulation are released, and the blood forms stable fibrin polymers under the combined action of endogenous and exogenous blood coagulation paths, so that blood clots are formed, and the purpose of wound hemostasis is achieved.

However, in the actual situation, the hemostasis of the major hemorrhage caused by the rupture of the aorta and the great vein is very difficult, and the hemostasis effect of the existing hemostatic materials is to be improved, so that a hemostatic material for trauma emergency treatment is needed to be researched.

Disclosure of Invention

Based on the technical problems in the background art, the invention provides a preparation method of a trauma emergency hemostatic material.

The technical scheme of the invention is as follows:

a preparation method of a hemostatic material for trauma emergency treatment comprises the following steps:

(1) dissolving gelatin in deionized water at 40-60 deg.C to obtain gelatin solution with mass concentration of 15-25%;

(2) adding three-dimensional porous double-crosslinked polyaniline/graphene composite xerogel into gelatin solution, and magnetically stirring at 300-400rpm for 20-30min to uniformly disperse the xerogel to obtain mixed solution;

(3) adding the mixed solution of the traditional Chinese medicine components into the mixed solution, pouring the mixed solution into a mold, keeping the liquid layer at the thickness of 5-8mm for 45-60min at normal temperature, discharging air bubbles, pre-freezing the mixed solution at the low temperature of minus 40-minus 25 ℃ for 1-1.5h, and freeze-drying the mixed solution in a vacuum freeze dryer to obtain mixed xerogel;

(4) and taking the mixed xerogel out of the die, respectively covering a layer of medical gauze on the upper surface and the lower surface, and tightly attaching to obtain the trauma emergency hemostatic material.

The preparation method of the three-dimensional porous double-crosslinked polyaniline/graphene composite xerogel refers to embodiment 1 of Chinese patent CN 110183718A.

Preferably, in the step (2), the addition amount of the three-dimensional porous double-crosslinked polyaniline/graphene composite xerogel is 0.1-0.3% of the mass of the gelatin solution.

Preferably, in the step (3), the amount of the mixed solution of the traditional Chinese medicine components is 1.8-3.5% of the mass of the gelatin solution.

Further preferably, the mixed solution of the traditional Chinese medicine components consists of the following components in percentage by weight: 2-5% of vaseline, 1-3% of sodium dodecyl sulfate, 3-6% of semisynthetic fatty glyceride, 0.1-0.3% of borneol, 1.8-2.5% of glycerol, 0.5-0.8% of pearl powder, 8-12% of distilled water and the balance of traditional Chinese medicine extract.

Preferably, the traditional Chinese medicine extracting solution is prepared by extracting the following traditional Chinese medicines with water: cortex Phellodendri, radix et rhizoma Rhei, rhizoma Polygoni Cuspidati, radix Saposhnikoviae, flos Sophorae Immaturus, radix Angelicae sinensis, Glycyrrhrizae radix, Bulbus Lilii, artificial Moschus, rhizoma Gymnadeniae and lignum Dalbergiae Odoriferae.

Preferably, in the traditional Chinese medicine extracting solution, the weight parts of the traditional Chinese medicine components are as follows: 5-20 parts of phellodendron, 5-20 parts of rhubarb, 2-10 parts of giant knotweed, 3-15 parts of divaricate saposhnikovia root, 3-15 parts of sophora flower, 8-25 parts of angelica, 15-25 parts of liquorice, 2-10 parts of lily, 2-10 parts of artificial musk, 5-20 parts of gymnadenia conopsea and 3-15 parts of dalbergia wood.

Further preferably, in the traditional Chinese medicine extracting solution, the weight parts of the traditional Chinese medicine components are as follows: 8-15 parts of phellodendron, 8-15 parts of rheum officinale, 5-8 parts of polygonum cuspidatum, 6-12 parts of divaricate saposhnikovia root, 6-12 parts of sophora flower, 10-18 parts of angelica sinensis, 15-25 parts of liquorice, 5-8 parts of lily, 5-8 parts of artificial musk, 10-18 parts of gymnadenia conopsea and 6-12 parts of rosewood heart wood.

Preferably, the preparation method of the mixed solution of the traditional Chinese medicine components comprises the following steps:

A. soaking cortex Phellodendri, radix et rhizoma Rhei, rhizoma Polygoni Cuspidati, radix Saposhnikoviae, flos Sophorae Immaturus, radix Angelicae sinensis, Glycyrrhrizae radix, Bulbus Lilii, rhizoma Gymnadeniae and lignum Dalbergiae Odoriferae in water, reflux-extracting twice, collecting the two water extractive solutions, mixing, and filtering; concentrating the filtrate to relative specific gravity of 1.05-1.10 to obtain Chinese medicinal ointment;

B. mixing vaseline and semisynthetic fatty glyceride, heating to dissolve, and keeping the temperature at 75-78 deg.C to obtain oil phase;

C. mixing sodium laurylsulfate, glycerol, distilled water and Chinese medicinal paste, heating, adding Borneolum Syntheticum and Margarita powder, stirring, and keeping the temperature at 60-65 deg.C to obtain water phase;

D. slowly adding the oil phase into the water phase, stirring, and cooling to 40-45 deg.C.

Preferably, in the step C, the particle size of the pearl powder and the borneol is 250 meshes.

Phellodendron (hu an ng b oa), traditional Chinese medicine name. Is dried bark of phellandron chinensis schneid of Rutaceae. It is mainly used for treating damp-heat dysentery, jaundice, dark urine, leukorrhagia, pudendal pruritus, pyretic stranguria, pain, tinea pedis, atrophic debility cramped, bone-steaming, overstrain, night sweat, spermatorrhea, pyocutaneous disease, toxic swelling, eczema, and eczema. Salted cortex phellodendri is used for nourishing yin and reducing internal heat. Can be used for treating hyperactivity of fire due to yin deficiency, night sweat and steaming bone.

Rhubarb, named as traditional Chinese medicine. The product is rhizome of Rheum palmatum L., Rheum tanguticum Maxim. or Rheum officinale Baill. of Polygonaceae. Selecting plants growing for more than 3 years in 9-10 months, digging rootstocks, cutting off stems, leaves and rootstocks, scraping off rough skin and terminal buds, air drying, oven drying or slicing and sun drying. The main functional indications are as follows: purging heat-toxin, breaking stagnation and removing blood stasis. Treating excessive heat constipation, delirium, fever, food stagnation, abdominal fullness, dysentery, tenesmus, stasis, amenorrhea, abdominal mass, epidemic heat, acute conjunctivitis, hematemesis, epistaxis, yang jaundice, edema, stranguria with turbid urine, carbuncle, sore, and injury due to fire and dampness. Purge heat and unblock intestines, cool blood and remove toxicity, dispel stasis and dredge meridians. Can be used for treating constipation due to excessive heat, abdominal pain due to stagnation, dysentery, and jaundice due to damp-heat.

Giant knotweed (academic name: Reynoutria japonica Houtt.): polygonaceae, Polygonum perennial herb. The rhizomes are thick, the stems are upright and the height can reach 2 meters, the rhizomes are hollow, the leaves are wide, oval or oval, the rhizomes are approximate to leathery, the two sides have no hair, the tops are tapered, the bases are wide, wedge-shaped, section-shaped or approximate to circular, the leaf supporting sheathing stroma is conical inflorescence, the parthenocarpy is, the male and female heterozygotes, and the axilla is grown; the bract is funnel-shaped, the flower quilt is light green, the lean fruit is egg-shaped, the fruit is glossy and black brown, the fruit blooms in 8-9 months, and the fruit can bear in 9-10 months. Has effects in promoting blood circulation, dispelling blood stasis, dredging channels, and relieving cough.

Divaricate Saposhnikovia divaricata (Trucz.) Schischk.) perennial herbs, thick roots, slender cylinders, light yellow-brown. Fang Feng is used as root medicine. Pungent and sweet in flavor and warm in nature. Has effects in relieving exterior syndrome, inducing perspiration, dispelling pathogenic wind, and removing dampness, and can be used for treating wind-cold type common cold, headache, fever, joint pain, tetanus, etc.

Flos sophorae, the name of traditional Chinese medicine. Is dried flower and bud of Sophora japonica L.of Leguminosae. It is mainly used for hematochezia, hemorrhoidal bleeding, bloody dysentery, metrorrhagia, hematemesis, epistaxis, liver heat with conjunctival congestion, headache and vertigo.

The Chinese angelica is named as dry angelica, Qinlian, Xi angelica, Min angelica, golden angelica, angelica body, culvert angelica tail, wendu, angelica starter and native angelica, and is herbaceous for many years and 0.4 to 1 meter higher. The flowering period is 6-7 months, and the fruit period is 7-9 months. The root of it can be used as a medicine, and is one of the most commonly used Chinese herbs. Has effects in invigorating qi, regulating menstruation, relieving pain, moistening dryness, smoothing intestine, resisting cancer, resisting aging, and enhancing immunity.

Licorice, name of Chinese medicine. Is dried root and rhizome of Glycyrrhiza uralensis Fisch, Glycyrrhiza inflata Bat, or Glycyrrhiza glabra L. Distributed in northeast, northeast China, Shaanxi, Gansu, Qinghai, Xinjiang, Shandong, etc. Has the effects of invigorating spleen and replenishing qi, clearing away heat and toxic materials, eliminating phlegm and stopping cough, relieving spasm and pain, and harmonizing the medicines. It is indicated for spleen and stomach deficiency, lassitude and hypodynamia, palpitation and shortness of breath, cough with profuse sputum, spasm and pain of abdomen and limbs, carbuncle and sore, and to relieve toxicity and strong action of drugs.

Bulbus Lilii is dried fleshy scale leaf of Lilium tigrinum Thunb, Lilium brownnii F.E.Brown var. viridumum Baker or Lilium pumilum DC. Has the effects of nourishing yin, moistening lung, clearing away heart-fire and tranquilizing mind. It is commonly indicated for dry cough due to yin deficiency, cough with blood, restlessness and pavor due to deficiency, insomnia and dreaminess, absentmindedness.

Artificial musk is used for fever unconsciousness, apoplexy with phlegm syncope, qi depression with sudden syncope, central aversion to coma, heart and abdomen pain, swelling and pain scrofula, sore throat and traumatic injury.

Gymnadenia conopsea (L.) R.Br.) of Monocotyledoneae, Orchidaceae, genus Gymnadenia, plant of 60 cm, fleshy elliptic tuber, lower palm-shaped division, upright stem, leaf linear shape coated with needle, long and narrow round or belt shape, total shape inflorescence, dense flower, bud sheet coated with needle, upright petal, pollen ball shaped egg ball, and disc-sticking linear shape coated with needle. In 6 to 7 months, spike-shaped inflorescences are extracted, pink flowers are bloomed, white flowers are small, and the flower is beautiful. Mature capsule with long round shape in 8-9 months. Can be used for treating kidney invigorating, essence replenishing, asthenia after illness, cough, leucorrhea, traumatic injury, blood stasis, and swelling and pain.

Dalbergia wood, name of traditional Chinese medicine. Is dried heartwood of trunk and root of Dalbergia odorifera T.Chen of Leguminosae. It is mainly used for hematemesis, epistaxis, traumatic hemorrhage, hypochondriac pain due to liver depression, thoracic obstruction, stabbing pain, traumatic injury, emesis and abdominal pain.

The invention has the advantages that: the invention relates to a trauma emergency hemostasis material, which comprises the following steps: dissolving gelatin in deionized water at 40-60 deg.C to obtain gelatin solution; adding three-dimensional porous double-crosslinked polyaniline/graphene composite xerogel into gelatin solution, uniformly dispersing the xerogel by magnetic stirring, adding a traditional Chinese medicine component mixed solution, pouring into a mold, standing at normal temperature and discharging air bubbles, pre-freezing at the low temperature of minus 40-minus 25 ℃, freeze-drying in a vacuum freeze dryer to obtain mixed xerogel, taking the mixed xerogel out of the mold, respectively covering a layer of medical gauze on the upper surface and the lower surface, and tightly attaching to obtain the trauma emergency hemostatic material. The traditional Chinese medicine component mixed liquor comprises the following components in percentage by weight: 2-5% of vaseline, 1-3% of sodium dodecyl sulfate, 3-6% of semisynthetic fatty glyceride, 0.1-0.3% of borneol, 1.8-2.5% of glycerol, 0.5-0.8% of pearl powder, 8-12% of distilled water and the balance of traditional Chinese medicine extract. Wherein the traditional Chinese medicine extracting solution is prepared by extracting the following traditional Chinese medicines with water: cortex Phellodendri, radix et rhizoma Rhei, rhizoma Polygoni Cuspidati, radix Saposhnikoviae, flos Sophorae Immaturus, radix Angelicae sinensis, Glycyrrhrizae radix, Bulbus Lilii, artificial Moschus, rhizoma Gymnadeniae and lignum Dalbergiae Odoriferae. The novel material can timely stop bleeding for various bleeding conditions, and the time required for stopping bleeding is generally less than 5 minutes. After the three-dimensional porous double-crosslinked polyaniline/graphene composite xerogel is added into the novel material, the drug effect can be obviously improved.

Detailed Description

Example 1

A preparation method of a hemostatic material for trauma emergency treatment comprises the following steps:

(1) dissolving gelatin in deionized water at 45 ℃ to prepare a gelatin solution with the mass concentration of 18%;

(2) adding three-dimensional porous double-crosslinked polyaniline/graphene composite xerogel into gelatin solution, and magnetically stirring for 22min at 350pm to uniformly disperse the xerogel to obtain mixed solution;

(3) adding the mixed solution of the traditional Chinese medicine components into the mixed solution, pouring the mixed solution into a mould, keeping the liquid layer at the thickness of 6mm, standing the mixed solution for 55min at normal temperature, discharging air bubbles, pre-freezing the mixed solution at the low temperature of-28 ℃ for 1.2h, and freeze-drying the pre-frozen mixed solution in a vacuum freeze-drying machine to obtain mixed xerogel;

(4) and taking the mixed xerogel out of the die, respectively covering a layer of 4mm medical gauze on the upper surface and the lower surface, and tightly attaching to obtain the trauma emergency hemostatic material.

Preferably, in the step (2), the addition amount of the three-dimensional porous double-crosslinked polyaniline/graphene composite xerogel is 0.18% of the mass of the gelatin solution.

Preferably, in the step (3), the amount of the mixed solution of the traditional Chinese medicine components is 2.5% of the mass of the gelatin solution.

The traditional Chinese medicine component mixed liquor comprises the following components in percentage by weight: 3.5 percent of vaseline, 1.8 percent of sodium dodecyl sulfate, 5.5 percent of semisynthetic fatty glyceride, 0.25 percent of borneol, 1.95 percent of glycerol, 0.7 percent of pearl powder, 10.5 percent of distilled water and the balance of traditional Chinese medicine extract.

In the traditional Chinese medicine extracting solution, the weight parts of the traditional Chinese medicine components are as follows: 12 parts of phellodendron, 12 parts of rhubarb, 7 parts of giant knotweed, 9 parts of divaricate saposhnikovia root, 9 parts of sophora flower, 15 parts of angelica, 18 parts of liquorice, 6 parts of lily, 6 parts of artificial musk, 15 parts of gymnadenia conopsea and 8 parts of rosewood.

The preparation method of the mixed liquid of the traditional Chinese medicine components comprises the following steps:

A. soaking cortex Phellodendri, radix et rhizoma Rhei, rhizoma Polygoni Cuspidati, radix Saposhnikoviae, flos Sophorae Immaturus, radix Angelicae sinensis, Glycyrrhrizae radix, Bulbus Lilii, rhizoma Gymnadeniae and lignum Dalbergiae Odoriferae in water, reflux-extracting twice, collecting the two water extractive solutions, mixing, and filtering; concentrating the filtrate to relative specific gravity of 1.08 to obtain Chinese medicinal ointment;

B. mixing vaseline and semisynthetic fatty glyceride, heating to dissolve, and keeping the temperature at 77 deg.C to obtain oil phase;

C. mixing sodium dodecyl sulfate, glycerol, distilled water and the Chinese medicinal paste, heating, adding Borneolum Syntheticum and Margarita powder, stirring, and keeping the temperature at 62 deg.C to obtain water phase;

D. slowly adding the oil phase into the water phase, stirring, and cooling to 43 deg.C.

In the step C, the particle sizes of the pearl powder and the borneol are 250 meshes.

Example 2

A preparation method of a hemostatic material for trauma emergency treatment comprises the following steps:

(1) dissolving gelatin in deionized water at 60 ℃ to prepare a gelatin solution with the mass concentration of 15%;

(2) adding the three-dimensional porous double-crosslinked polyaniline/graphene composite xerogel into the gelatin solution, and magnetically stirring at 400rpm for 20min to uniformly disperse the xerogel to obtain a mixed solution;

(3) adding the mixed solution of the traditional Chinese medicine components into the mixed solution, pouring the mixed solution into a mold, keeping the liquid layer at the thickness of 8mm for 45min at normal temperature, discharging air bubbles, pre-freezing the mixed solution at the low temperature of minus 40 ℃ for 1h, and freeze-drying the pre-frozen solution in a vacuum freeze-drying machine to obtain mixed xerogel;

(4) and taking the mixed xerogel out of the die, respectively covering a layer of 4mm medical gauze on the upper surface and the lower surface, and tightly attaching to obtain the trauma emergency hemostatic material.

Preferably, in the step (2), the addition amount of the three-dimensional porous double-crosslinked polyaniline/graphene composite xerogel is 0.1% of the mass of the gelatin solution.

Preferably, in the step (3), the amount of the mixed solution of the traditional Chinese medicine components is 3.5% of the mass of the gelatin solution.

The traditional Chinese medicine component mixed liquor comprises the following components in percentage by weight: 5% of vaseline, 1% of sodium dodecyl sulfate, 6% of semisynthetic fatty glyceride, 0.1% of borneol, 2.5% of glycerol, 0.5% of pearl powder, 12% of distilled water and the balance of traditional Chinese medicine extract.

In the traditional Chinese medicine extracting solution, the weight parts of the traditional Chinese medicine components are as follows: 15 parts of phellodendron, 8 parts of rhubarb, 8 parts of giant knotweed, 6 parts of divaricate saposhnikovia root, 12 parts of sophora flower, 10 parts of angelica, 25 parts of liquorice, 5 parts of lily, 8 parts of artificial musk, 18 parts of gymnadenia conopsea and 6 parts of rosewood.

The preparation method of the mixed liquid of the traditional Chinese medicine components comprises the following steps:

A. soaking cortex Phellodendri, radix et rhizoma Rhei, rhizoma Polygoni Cuspidati, radix Saposhnikoviae, flos Sophorae Immaturus, radix Angelicae sinensis, Glycyrrhrizae radix, Bulbus Lilii, rhizoma Gymnadeniae and lignum Dalbergiae Odoriferae in water, reflux-extracting twice, collecting the two water extractive solutions, mixing, and filtering; concentrating the filtrate to relative specific gravity of 1.10 to obtain Chinese medicinal ointment;

B. mixing vaseline and semisynthetic fatty glyceride, heating to dissolve, and keeping the temperature at 75 deg.C to obtain oil phase;

C. mixing sodium dodecyl sulfate, glycerol, distilled water and the Chinese medicinal paste, heating, adding Borneolum Syntheticum and Margarita powder, stirring, and keeping the temperature at 65 deg.C to obtain water phase;

D. slowly adding the oil phase into the water phase, stirring, and cooling to 40 deg.C.

In the step C, the particle sizes of the pearl powder and the borneol are 250 meshes.

Example 3

A preparation method of a hemostatic material for trauma emergency treatment comprises the following steps:

(1) dissolving gelatin in deionized water at 40 ℃ to prepare gelatin solution with mass concentration of 25%;

(2) adding the three-dimensional porous double-crosslinked polyaniline/graphene composite xerogel into the gelatin solution, and magnetically stirring at 300rpm for 30min to uniformly disperse the xerogel to obtain a mixed solution;

(3) adding the mixed solution of the traditional Chinese medicine components into the mixed solution, pouring the mixed solution into a mold, keeping the liquid layer at the thickness of 5mm for 60min at normal temperature, discharging air bubbles, pre-freezing the mixed solution at the low temperature of minus 25 ℃ for 1.5h, and freeze-drying the mixed solution in a vacuum freeze-drying machine to obtain mixed xerogel;

(4) and taking the mixed xerogel out of the die, respectively covering a layer of 5mm medical gauze on the upper surface and the lower surface, and tightly attaching to obtain the trauma emergency hemostatic material.

Preferably, in the step (2), the addition amount of the three-dimensional porous double-crosslinked polyaniline/graphene composite xerogel is 0.3% of the mass of the gelatin solution.

Preferably, in the step (3), the amount of the mixed solution of the traditional Chinese medicine components is 1.8% of the mass of the gelatin solution.

The traditional Chinese medicine component mixed liquor comprises the following components in percentage by weight: 2% of vaseline, 3% of sodium dodecyl sulfate, 3% of semisynthetic fatty glyceride, 0.3% of borneol, 1.8% of glycerol, 0.8% of pearl powder, 8% of distilled water and the balance of traditional Chinese medicine extract.

In the traditional Chinese medicine extracting solution, the weight parts of the traditional Chinese medicine components are as follows: 8 parts of phellodendron, 15 parts of rhubarb, 5 parts of giant knotweed, 12 parts of divaricate saposhnikovia root, 6 parts of sophora flower, 18 parts of angelica, 15 parts of liquorice, 8 parts of lily, 5 parts of artificial musk, 10 parts of gymnadenia conopsea and 12 parts of rosewood.

The preparation method of the mixed liquid of the traditional Chinese medicine components comprises the following steps:

A. soaking cortex Phellodendri, radix et rhizoma Rhei, rhizoma Polygoni Cuspidati, radix Saposhnikoviae, flos Sophorae Immaturus, radix Angelicae sinensis, Glycyrrhrizae radix, Bulbus Lilii, rhizoma Gymnadeniae and lignum Dalbergiae Odoriferae in water, reflux-extracting twice, collecting the two water extractive solutions, mixing, and filtering; concentrating the filtrate to relative specific gravity of 1.05-1.10 to obtain Chinese medicinal ointment;

B. mixing vaseline and semisynthetic fatty glyceride, heating to dissolve, and keeping the temperature at 75-78 deg.C to obtain oil phase;

C. mixing sodium laurylsulfate, glycerol, distilled water and Chinese medicinal paste, heating, adding Borneolum Syntheticum and Margarita powder, stirring, and keeping the temperature at 60-65 deg.C to obtain water phase;

D. slowly adding the oil phase into the water phase, stirring, and cooling to 40-45 deg.C.

In the step C, the particle sizes of the pearl powder and the borneol are 250 meshes.

Comparative example 1

The gymnadenia conopsea in the traditional Chinese medicine extracting solution is removed, and the rest proportion and the preparation method are the same as the embodiment 1.

Comparative example 2

The gymnadenia conopsea in the traditional Chinese medicine extracting solution is replaced by eucommia ulmoides, and the rest proportion and the preparation method are the same as the embodiment 1.

Comparative example 3

The dalbergia wood in the traditional Chinese medicine extracting solution is removed, and the rest proportion and the preparation method are the same as the embodiment 1.

Comparative example 4

The rosewood heart wood in the traditional Chinese medicine extracting solution is replaced by the pseudo-ginseng, and the rest proportion and the preparation method are the same as the embodiment 1.

Comparative example 5

The gymnadenia conopsea and the dalbergia wood in the traditional Chinese medicine extracting solution are removed at the same time, and the rest proportion and the preparation method are the same as the embodiment 1.

Comparative example 6

The three-dimensional porous double-crosslinked polyaniline/graphene composite xerogel is replaced by graphene oxide, and the rest proportion and preparation are the same as those in the embodiment 1.

Comparative example 7

The three-dimensional porous double-crosslinked polyaniline/graphene composite xerogel is removed, and the rest proportion and preparation are the same as those in the embodiment 1.

Test example

The following novel materials of examples 1-3 and comparative examples 1-6 were applied to porcine femoral artery hemostasis and the wound healing time was measured every 1 minute from 3 minutes (wound healing time was counted for pigs with healed wounds for 20 minutes only, and non-healed pigs were not counted) as follows: 50 100 +/-2 kg of long white pigs are selected in each example (the male-female ratio is about 1:1, and no obvious difference exists among the examples); making a femoral artery incision with the length of 3 +/-0.2 cm by using a thin blade; the new material was immediately applied to the wound and finger pressed. The Yunnan white powder and thick gauze are adopted and finger pressure is carried out to be used as a control example; the specific test data are shown in Table 1.

Table 1: the hemostatic effect contrast detection data is applied to the femoral artery of the pig;

	total number of pigs	Number of healed wound	Wound healing time (min)
				Example 1	50	50	4.5
Example 2	50	50	4.7
				Example 3	50	50	4.4
Comparative example 1	50	45	7.1
				Comparative example 2	50	46	6.5
Comparative example 3	50	48	6.2
				Comparative example 4	50	50	6.1
Comparative example 5	50	41	7.9
				Comparative example 6	50	37	8.7
Comparative example 7	50	34	9.1
				Comparative example	50	46	10.4

Remarking: the wound healing means that blood does not seep within 10s under the condition of not pressing the wound.

From the above test data, it can be known that the novel material of the present invention, applied to hemostasis, can significantly shorten the wound healing time.

The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.