阅读说明：本技术 串联柱固相萃取系统及其操作方法 (Series column solid phase extraction system and operation method thereof ) 是由 彭德义 姜小燕 徐进勇 王彤 蔡秀丽 孙傲 王乐然 路玉沁 于 2021-08-11 设计创作，主要内容包括：一种串联柱固相萃取系统及其操作方法,包括储液瓶阵列、注射泵、选择阀、分配阀、第一、第二、第三级三通阀组V1、V2、V3、样品管阵列、多级萃取柱阵列、缓冲管阵列、样品收集管阵列和一个废液桶,以及自动控制单元等。本系统由多个相互独立、并列的萃取通道组成,每个萃取通道包括独立的样品管、萃取柱和缓冲管。所述样品管阵列、缓冲管阵列固定在各自的支撑架上,在电机驱动下可上下翻转。本系统可对多个样品自动完成两级串联固相萃取的所有操作,具有省时省力的特点。(A serial column solid phase extraction system and an operation method thereof comprise a liquid storage bottle array, an injection pump, a selection valve, a distribution valve, a first, a second and a third three-stage valve group V1, V2, V3, a sample tube array, a multistage extraction column array, a buffer tube array, a sample collection tube array, a waste liquid barrel, an automatic control unit and the like. The system consists of a plurality of mutually independent and parallel extraction channels, and each extraction channel comprises an independent sample tube, an extraction column and a buffer tube. The sample tube array and the buffer tube array are fixed on respective supporting frames and can be turned over up and down under the drive of a motor. The system can automatically complete all operations of two-stage series solid-phase extraction on a plurality of samples, and has the characteristics of time saving and labor saving.)

1. A series column solid phase extraction system comprises an automatic control unit, and is characterized in that a plurality of liquid storage bottles for respectively storing cleaning reagents, leacheate or eluent and an outlet of an air filter G are respectively connected with a gating end of a selection valve CV1 through pipelines, a common end of the selection valve CV1 is communicated with an injector (1), other gating ends of the selection valve CV1 are connected with a common end of a distribution valve DV1, a plurality of common ends of a first-stage three-way valve group V1 are respectively connected with a gating end of the distribution valve DV1 through pipelines, a plurality of 1-th opening ends of a first-stage three-way valve group V1 are respectively connected with an inlet of a sample tube (2), and inlet covers are respectively screwed on inlets of the sample tubes (2); a plurality of 2 nd opening ends of the first-stage three-way valve group V1 are respectively connected with a second opening end of the third-stage three-way valve group V3, outlets of a plurality of sample tubes (2) are respectively connected with a first solid phase extraction column (4) and a common end of the second-stage three-way valve group V2 in sequence, a plurality of first opening ends of the second-stage three-way valve group V2 are respectively communicated with a waste liquid barrel (8) through pipelines, a plurality of second opening ends of the second-stage three-way valve group V2 are respectively connected with a first opening end of the third-stage three-way valve group V3, and a plurality of common ends of the third-stage three-way valve group V3 are respectively connected with a buffer tube, a second-stage solid phase extraction column (6) and a sample collection cup (7) in sequence through pipelines; also has a roll-over stand (3): the horizontal shaft is rotatably erected on the left bearing and the right bearing, the shaft of the stepping motor is connected with the horizontal shaft through a coupler, and the sample tubes (2) are fixed on the horizontal shaft along the vertical direction.

2. The series column solid phase extraction system of claim 1, further comprising a plurality of buffer tubes (5) having the same number as the sample tubes (2), wherein the buffer tubes (5) are connected between the common end of the third stage three-way valve set V3 and the second stage solid phase extraction column (6); and a second roll-over stand (3 ') with the same structure as the roll-over stand (3), and a plurality of buffer tubes (5) are fixed on the second roll-over stand (3').

3. A series column solid phase extraction system according to claim 1 or claim 2 wherein the selection valve CV1 further has a gate end in line communication with the waste drum (8).

4. The series column solid phase extraction system of claim 3, wherein the number of said liquid storage bottles is 7, said selection valve CV1 has 10 gate ends A, B, C, D, E, F, G, H, I, J; said distribution valve DV1 has 10 gating ends a ', B', C ', D', E ', F', G ', H', I ', J'; the number of the sample tubes (2), the first-stage solid phase extraction column (4), the second-stage solid phase extraction column (6), the buffer tube (5), the first-stage three-way valve group V1, the second-stage three-way valve group V2 and the third-stage three-way valve group V3 is 10, and the number of the sample collection cups (7) can be set as required; all the three-way valves are controllable three-way valves (including pneumatic three-way valves, electromagnetic three-way valves and the like).

5. The series column solid phase extraction system of claim 4, wherein inlets and outlets of the sample tube (2) and the buffer tube (5) are connected with corresponding pipelines by luer connectors; all the lines are composed of a flexible tube which can be repeatedly bent.

6. A method of operating a solid phase extraction system as claimed in claim 5, comprising the steps of:

firstly, preparing liquid storage bottles R1-R7: connecting the corresponding liquid storage bottle with the corresponding gating end of a selector valve CV 1;

second, the sample is loaded into sample tube (2): opening the inlet covers of all the sample tubes, filling all the sample solutions to be processed into the corresponding sample tubes, sealing all the sample inlet covers, and keeping the sample tubes vertical with the inlets of the sample tubes upward;

thirdly, loading a sample into the first-stage extraction column (4): switching the common end of the selector valve CV1 to the gate end connected to the air filter, the injector (1) sucking air into the cylinder through the common end of the selector valve; the common end of the selector valve is switched to a designated gate end, and the gate end of the selector valve is connected with the common end of the distribution valve DV 1; then the common end of the distribution valve is switched to the corresponding gating end, and the gating end is connected with the common end of the corresponding three-way valve in the rear first-stage three-way valve group V1; the common end of the three-way valves is switched to the first opening end, the first opening of the three-way valves is connected with the inlet of the corresponding sample tube (2), and the outlet of the sample tube is connected with the inlet of the first-stage extraction column (4); the outlet of the first-stage extraction column is connected with the common end of a second-stage three-way valve group V2;

after the state switching of the selector valve CV1, the distribution valve DV1, the first-stage three-way valve V1 and the second-stage three-way valve V2 is completed, a push rod of the injector (1) is pushed to push the air sucked into the syringe in front into the sample tube (2), the pressure in the sample tube is increased, and then the sample in the sample tube is pressed into the first-stage extraction column (4); the components flowing out of the outlet of the first-stage extraction column are introduced into a buffer tube (5) through a second opening end of a second-stage three-way valve group V2 and a first opening end and a common end of a third-stage three-way valve group V3 or introduced into a waste liquid barrel (8) through a first opening end of the second-stage three-way valve group according to requirements; at this point, the operation of loading the first batch of samples into the first-stage extraction column (4) is completed;

fourthly, cleaning the residual sample on the inner wall of the sample tube (2), and loading the cleaning liquid into the first-stage extraction column (4): the specific operation steps are that a driving motor, namely a stepping motor, of the sample tube turning frame (3) turns the sample tube (2) upwards by 180 degrees, so that the outlet of the sample tube (2) is upward and the inlet of the sample tube is downward; then according to the operation step of the third step, the injector (1) sucks cleaning reagent from the liquid storage bottle selected from the liquid storage bottles R1-R7 through one gating end of a selection valve CV1, and then pushes the reagent into the sample tube (2) through a distribution valve DV1 and a first-stage three-way valve group V1; then the turnover frame (3) is turned over for a plurality of times, and the reagent in the sample tube cleans the inner wall of the sample tube (2); after the sample tube is cleaned, the inlet of the sample tube (2) is upward, and the outlet is downward; then according to the operation steps of the third step: the gating end of the selector valve (2) is switched to the gating end connected with the air filter, air is sucked in by the injector, and then pushed into the sample tube (2) through the distribution valve DV1 and the first-stage three-way valve group V1, the pressure in the sample tube (2) is increased, and a reagent for cleaning the sample tube is pressed into the first-stage extraction column (4); the above cleaning steps are repeated for a plurality of times according to actual conditions;

fifthly, solid-phase extraction, leaching and elution operations of the first-stage extraction column (4): according to the third step or the fourth step similar operation steps, a required eluent or eluent is sucked from a bottle corresponding to a liquid storage bottle R1-R7 by an injector (1) through a corresponding gating end of a selector valve CV1, and then the eluent and the eluent with required types and required volumes are sequentially injected into a first-stage extraction column (4) through a distribution valve DV1 first-stage three-way valve group V1 and a sample tube (2), so that the sample in the first-stage extraction column (4) is subjected to solid phase extraction elution and elution; wherein, the elution component that flows out from the first-stage extraction column (4) export is the waste liquid of solid phase extraction and discharges into the waste liquid bucket, the elution component that first-stage extraction column (4) export is the target thing of solid phase extraction and flows into in buffer tube (6) through the second open end of second level three-way valve group V2 and with the first open end and the public end of third level three-way valve group V3, or another mode is: the eluted components of the first stage extraction column (4) do not pass through a buffer tube, but directly flow into the second stage extraction column (6) through the common end of V3;

sixthly, chemical treatment of the solution in the buffer tube (5): firstly, the buffer tube (5) is turned over by 180 degrees through a driving motor of a buffer tube turning frame (3') so that the outlet of the buffer tube is upward and the inlet of the buffer tube is downward; then according to the third step or the fourth step or the fifth step similar operation steps, the injector (1) sucks reagent from a corresponding liquid storage bottle from the liquid storage bottles R1-R7 through the gating end of the selector valve CV1, and then injects the reagent into the buffer tube (5) through the distributing valve DV1, the common end-the second opening end of the first-stage three-way valve group and the second opening end-the common end of the third-stage three-way valve group in sequence; then the buffer tube (5) is turned over for many times to fully mix and react the solution in the buffer tube; after the solution in the buffer tube (5) is uniformly mixed and the reaction is finished, the outlet of the buffer tube (5) faces downwards and the inlet faces upwards;

seventhly, loading the solution in the buffer tube into a second-stage extraction column (6): according to the operation steps similar to the third step, the fourth step, the fifth step or the sixth step, the injector (1) sucks air through a gating end of a selection valve CV1 connected with an air filter, then pushes the air into the buffer tube (5) through the common end-the second open end of the first-stage three-way valve group V1 and the second open end-the common end of the third-stage three-way valve group V3 again, the pressure of the buffer tube (5) is increased, so that the solution in the buffer tube (5) is pressed into the second-stage extraction column (6), and the components flowing out of the outlet of the second-stage extraction column (6) in the process of loading the solution are discharged into a waste liquid barrel or a sample collecting cup (7);

and step eight, the leaching and elution operation of the second-stage extraction column (6): according to the third step, the fourth step, the fifth step, the sixth step or the seventh step, which are similar operation steps, the injector (1) sucks a reagent from a corresponding liquid storage bottle from R1-R7 through the gating end of the selector valve CV1, then injects the reagent into the second-stage extraction column (6) through the distribution valve DV1, the common end-the second open end of the first-stage three-way valve group, the second open end-the common end of the third-stage three-way valve group and the buffer pipe (5) in sequence, and leacheate or elution components flowing out of the outlet of the second-stage extraction column (6) flow into a waste liquid barrel (8) or a collection cup (7) according to needs.

Technical Field

The invention relates to the technical field of chemical analysis pretreatment instruments, in particular to a multi-stage series column solid phase extraction system.

Background

Solid phase extraction is a chemical separation and enrichment technology of samples, and is widely applied to industries such as medical treatment, food, materials, chemical industry, geological metallurgy and the like. Compared with the solvent extraction separation and enrichment technology, the solid phase extraction technology has the advantages of reusable extraction agent, high recovery rate, simple and convenient operation, easy automation and the like. The existing automatic solid phase extraction instrument only has a first-stage solid phase extraction column, and a sample can only complete one-time solid phase extraction separation. When the sample matrix is complex, the number of interference components is large, and the purity requirement of the separated and purified components is high, the requirement is difficult to be met by one-time solid-phase extraction separation; if a single-stage (one-stage) solid phase extraction instrument is used, each extraction operation needs manual participation, and components obtained by the last extraction are subjected to intermediate treatment (such as evaporation concentration, re-dissolution, sample loading and the like), so that the operation is complicated and time-consuming, and the pollution risk is increased.

Disclosure of Invention

The invention aims to provide a series column solid phase extraction system, aiming at automatically completing all operations of a two-stage solid phase extraction process in one set of system: cleaning a sample tube, leaching and eluting an extraction column, collecting eluent to a waste liquid barrel, carrying out automatic online chemical treatment on solution in a buffer tube, collecting elution components of eluent to a sample collecting cup, and collecting extraction waste liquid.

The purpose of the invention is realized as follows: a series column solid phase extraction system comprises an automatic control unit, a plurality of liquid storage bottles for respectively storing cleaning reagents, leacheate or eluent and an air filter G outlet are respectively connected with a gating end of a selection valve CV1 through pipelines, a common end of a selection valve CV1 is communicated with an injector, a gated end of the selection valve CV1 is connected with a common end of a distribution valve DV1, a plurality of common ends of a first-stage three-way valve group V1 are respectively connected with a gating end of the distribution valve DV1 through pipelines, a plurality of 1 st opening ends of a first-stage three-way valve group V1 are respectively connected with an inlet of a sample tube, and the inlets of the sample tubes are respectively screwed with an inlet; a plurality of 2 nd opening ends of the first-stage three-way valve group V1 are respectively connected with a second opening end of the third-stage three-way valve group V3, outlets of a plurality of sample tubes are respectively connected with a first-stage solid phase extraction column and a common end of the second-stage three-way valve group V2 in sequence, a plurality of first opening ends of the second-stage three-way valve group V2 are respectively communicated with a waste liquid barrel through pipelines, a plurality of second opening ends of the second-stage three-way valve group V2 are respectively connected with a first opening end of the third-stage three-way valve group V3, and a plurality of common ends of the third-stage three-way valve group V3 are respectively connected with a second-stage solid phase extraction column and a sample collection cup in sequence through pipelines; also has a roll-over stand: the horizontal axis is rotatably erected on the left bearing and the right bearing, the shaft of the stepping motor is connected with the horizontal axis through the coupler, and the plurality of sample tubes are fixed on the horizontal axis along the vertical direction. A series column solid phase extraction system also comprises a plurality of buffer tubes with the same number as the sample tubes, and the buffer tubes are connected between the common end of a third-stage three-way valve group V3 and a second-stage solid phase extraction column; the buffer tube type buffer tube overturning machine is also provided with a second overturning frame with the same structure as the overturning frame, and a plurality of buffer tubes are fixed on the second overturning frame.

In a series column solid phase extraction system, the selection valve CV1 also has a gating end communicated with a waste liquid barrel through a pipeline.

A tandem column solid phase extraction system, the stock solution bottle is 7, the selection valve CV1 has 10 gating ends A, B, C, D, E, F, G, H, I, J; said distribution valve DV1 has 10 gating ends a ', B', C ', D', E ', F', G ', H', I ', J'; the number of the sample tubes, the number of the first-stage solid phase extraction columns, the number of the second-stage solid phase extraction columns, the number of the buffer tubes, the number of the first-stage three-way valve group V1, the number of the second-stage three-way valve group V2, the number of the third-stage three-way valve group V3 and the number of the sample collection cups are all 10; all the three-way valves can be of the type of automatically controllable three-way valves such as electromagnetic three-way valves or pneumatic three-way valves.

Inlets and outlets of the sample tube and the buffer tube are connected with corresponding pipelines by luer connectors; all the pipelines are composed of hoses.

It is a further object of the present invention to provide a method of operating the above system.

Yet another object of the present invention is achieved by: a method of operating a solid phase extraction system comprising the steps of:

firstly, preparing liquid storage bottles R1-R7: connecting the liquid storage bottles corresponding to various reagents required by the prepared solid-phase extraction with the corresponding gating ends of a selection valve CV 1;

second, sample is loaded into sample tubes: opening the inlet covers of all the sample tubes, filling all the sample solutions to be processed into the corresponding sample tubes, sealing all the sample inlet covers, and keeping the sample tubes vertical with the sample tube inlets on the upper side;

third, the first batch of samples is loaded into the first stage extraction column: switching the common terminal of the selector valve CV1 to the gate terminal connected to the air filter, the injector sucking air into the cylinder through the common terminal of the selector valve; the common end of the selector valve is switched to a designated gate end, and the gate end of the selector valve is connected with the common end of the distribution valve DV 1; then the common end of the distribution valve is switched to the corresponding gating end, and the gating end is connected with the common end of the corresponding three-way valve in the rear first-stage three-way valve group V1; the common end of the three-way valves is switched to the first opening end, the first opening is connected with the inlet of the corresponding sample tube, and the outlet of the sample tube is connected with the inlet of the first-stage extraction column; the outlet of the first-stage extraction column is connected with the common end of a second-stage three-way valve group V2;

after the state switching of the selector valve CV1, the distribution valve DV1, the first-stage three-way valve V1 and the second-stage three-way valve V2 is completed, the injector push rod is pushed to push the air sucked into the syringe in front into the sample tube, the pressure in the sample tube is increased, and then the sample in the sample tube is pressed into the first-stage extraction column; the components flowing out of the outlet of the first-stage extraction column are introduced into a buffer tube through a second opening end of a second-stage three-way valve group V2 and a first opening end and a common end of a third-stage three-way valve group V3 or introduced into a waste liquid barrel through a first opening end of a second-stage three-way valve group of the second-stage three-way valve group as required; so far, the operation of loading the first batch of samples into the first-stage extraction column is completed;

fourthly, cleaning the residual sample on the inner wall of the sample tube, and loading cleaning liquid into the first-stage extraction column: the specific operation steps are that a driving motor of the sample tube turning frame, namely a stepping motor, turns the sample tube upwards by 180 degrees, so that the outlet of the sample tube is upward and the inlet of the sample tube is downward; then according to the operation step of the third step, the injector sucks cleaning reagent from the liquid storage bottle selected from the liquid storage bottles R1-R7 through one gating end of a selection valve CV1, and then pushes the reagent into the sample tube through a distribution valve DV1 and a first-stage three-way valve group V1; then the turnover frame is turned over for many times, and the reagent in the sample tube cleans the inner wall of the sample tube; after the sample tube is cleaned, the inlet of the sample tube is upward, and the outlet of the sample tube is downward; then according to the operation steps of the third step: the gating end of the selector valve is switched to the gating end connected with the air filter, air is sucked in by the injector, the air is pushed into the sample tube through the distribution valve DV1 and the first-stage three-way valve group V1, the pressure in the sample tube is increased, and a reagent for cleaning the sample tube is pressed into the first-stage extraction column; the above cleaning steps are repeated for a plurality of times according to actual conditions;

fifthly, the first-stage extraction column solid phase extraction leaching and elution operation: according to the similar operation steps of the third step or the fourth step, a required leacheate or eluent is absorbed from a bottle corresponding to a liquid storage bottle R1-R7 by an injector through a corresponding gating end of a selection valve CV1, and then the leacheate and the eluent with required types and required volumes are sequentially injected into a first-stage extraction column through a first-stage three-way valve group V1 of a distribution valve DV1 and a sample tube 2, so that a sample in the first-stage extraction column is leached and eluted by solid-phase extraction; wherein, the elution component that flows out from first order extraction column outlet is the waste liquid discharge of solid phase extraction and goes into the waste liquid bucket promptly, and the elution component that first order extraction column outlet flows out promptly the target thing of solid phase extraction through the second open end of second level three-way valve group V2 and with the first open end and the public end inflow buffer tube of third level three-way valve group V3, or another mode is: the eluted components of the first stage extraction column did not pass through the buffer tube but flowed directly into the second stage extraction column through the common port of V3;

sixthly, chemically treating the solution in the buffer tube: firstly, the buffer tube is overturned for 180 degrees by a driving motor of a buffer tube overturning frame, so that the outlet of the buffer tube is upward and the inlet of the buffer tube is downward; then according to the third step or the fourth step or the fifth step similar operation steps, the injector sucks a reagent from a corresponding liquid storage bottle from the liquid storage bottles R1-R7 through the gating end of the selector valve CV1, and then injects the reagent into the buffer tube through the distributing valve DV1, the common end-the second opening end of the first-stage three-way valve group and the second opening end-the common end of the third-stage three-way valve group in sequence; then, turning the buffer tube up and down for many times so as to fully mix and react the solution in the buffer tube; after the solution in the buffer tube is uniformly mixed and the reaction is finished, the outlet of the buffer tube 5 is downward and the inlet is upward;

seventhly, loading the solution in the buffer tube into a second-stage extraction column: according to the operation steps similar to the third step, the fourth step, the fifth step or the sixth step, the injector sucks air through a gating end connected with the air filter through a selector valve CV1, then pushes the air into a buffer tube through the common end-the second open end of the first-stage three-way valve group V1 and the second open end-the common end of the third-stage three-way valve group V3 again, the pressure of the buffer tube is increased, so that the solution in the buffer tube is pressed into the second-stage extraction column, and the components flowing out of the outlet of the second-stage extraction column in the solution loading process are discharged into a waste liquid barrel or a sample collection cup;

step eight, the second-stage extraction column leaching and elution operation: according to the third step, the fourth step, the fifth step, the sixth step or the seventh step, similar operation steps are carried out, the injector sucks a reagent from a corresponding liquid storage bottle from R1-R7 through a gating end of a selector valve CV1, then the reagent is injected into the second-stage extraction column through a distribution valve DV1, a common end-second open end of the first-stage three-way valve group, a second open end-common end of the third-stage three-way valve group and a buffer pipe in sequence, and a leaching component or an elution component flowing out of an outlet of the second-stage extraction column flows into a waste liquid barrel or a collection cup as required.

Compared with the prior art, the invention has the beneficial effects that:

1. the invention can automatically complete the operation of solid phase extraction of two or more stages of series columns (as shown in figure 3), thereby reducing the labor cost of the extraction operation.

2. The invention can automatically complete all the operations related to two-stage or more than two-stage solid phase extraction, and the sample tube can be automatically turned over to realize the cleaning of the sample tube.

3. The buffer tube is used for storing specific components of the first-stage extraction column, and the buffer tube can also be automatically turned over and realizes automatic online chemical treatment in the buffer tube. .

4. According to the solid phase extraction system, the sample tube is always in a fully closed state in the extraction process, and is not easy to be polluted.

Drawings

FIG. 1 is a plan view of ten typical channel operating principles without buffer tubes

FIG. 2 is a plan view of ten typical channel operating principles including buffer tubes

FIG. 3 is a plan view of the working principle of forty channels of four multi-position switching valve sets

1-syringe or syringe pump; R1-R7 liquid storage bottle; g-an air filter; CV1, CV2 selector valve; DV1, DV 2-dispensing valve; v1-first stage three-way valve set; v2-second stage three-way valve set; v3-third stage three-way valve set; 2-sample tube can be inverted; 3. a 3' -roll-over stand and a second roll-over stand (the two roll-over stands have the same structure and the motor driving mechanisms of the two roll-over stands are the same); 4-first stage solid phase extraction column; 5-a buffer tube; 6-a second-stage solid phase extraction column; 7-a sample collection cup; 8-waste liquid barrel.

Detailed Description

The following detailed description of the principles and specific processes of the present invention is provided for a more detailed understanding of the present invention.

In the drawings, the right ends of all three-way valves are referred to as first open ends, and the left ends are referred to as second open ends.

A series column solid phase extraction system comprises an automatic control unit, a plurality of liquid storage bottles for respectively storing cleaning reagents, leacheate or eluent and an air filter G, wherein outlets of the air filter G are respectively connected with a gating end of a selection valve CV1 through pipelines, a common end of the selection valve CV1 is communicated with an injector 1, a gated end of the selection valve CV1 is connected with a common end of a distribution valve DV1, a plurality of common ends of a first-stage three-way valve group V1 are respectively connected with a gating end of the distribution valve DV1 through pipelines, a plurality of 1 st opening ends of a first-stage three-way valve group V1 are respectively connected with an inlet of a sample tube 2, and inlet covers are screwed on inlets of the sample tubes 2; a plurality of 2 nd opening ends of the first-stage three-way valve group V1 are respectively connected with a second opening end of the third-stage three-way valve group V3, outlets of a plurality of sample tubes 2 are respectively connected with a common end of a first-stage solid phase extraction column 4 and a second-stage three-way valve group V2 in sequence, a plurality of first opening ends of the second-stage three-way valve group V2 are respectively communicated with a waste liquid barrel 8 through pipelines, a plurality of second opening ends of the second-stage three-way valve group V2 are respectively connected with a first opening end of the third-stage three-way valve group V3, and a plurality of common ends of the third-stage three-way valve group V3 are respectively connected with a second-stage solid phase extraction column 6 and a sample collection cup 7 in sequence through pipelines; also has a roll-over stand 3: the horizontal axis is rotatably erected on the left bearing and the right bearing, the shaft of the stepping motor is connected with the horizontal axis through a coupler, and the sample tubes 2 are fixed on the horizontal axis along the vertical direction.

A series column solid phase extraction system also has a plurality of buffer tubes 5 with the same number as the sample tubes 2, the buffer tubes 5 are connected between the common end of a third stage three-way valve group V3 and a second stage solid phase extraction column 6; there is also a second roll-over stand 3 'of the same construction as the roll-over stand, to which a number of buffer tubes 5 are fixed 3'.

In a series column solid phase extraction system, the selection valve CV1 also has a gate end connected to the waste liquid barrel 8 via a pipeline.

A tandem column solid phase extraction system, the stock solution bottle is 7, the selection valve CV1 has 10 gating ends A, B, C, D, E, F, G, H, I, J; said distribution valve DV1 has 10 gating ends a ', B', C ', D', E ', F', G ', H', I ', J'; the number of the sample tubes 2, the number of the first-stage solid-phase extraction columns 4, the number of the second-stage solid-phase extraction columns 6, the number of the buffer tubes 5, the number of the first-stage three-way valve groups V1, the number of the second-stage three-way valve groups V2, the number of the third-stage three-way valve groups V3 and the number of the sample collection cups 7 are all 10; all the three-way valves are electric three-way valves (see fig. 1 and 2).

Inlets and outlets of the sample tube 2 and the buffer tube 5 are connected with corresponding pipelines by luer connectors; all of the lines are composed of a flexible tube that can be repeatedly bent.

The invention provides a system capable of carrying out two-stage series column automatic solid phase extraction on a sample, and aims to solve the technical problems of a single-stage extraction column solid phase extraction system in the technical background.

The invention relates to a series column solid phase extraction system, which comprises nine main units, namely a liquid storage bottle, an injection pump, a selection valve group, a distribution valve group, a three-way valve group, a sample tube, a series extraction column, a buffer tube, a collecting tube and a waste liquid barrel. Each sample to be processed corresponds to an independent solid phase extraction channel, and each independent solid phase extraction channel comprises an independent sample tube, independent extraction columns (a first stage and a second stage) connected in series and an independent buffer tube.

The liquid storage bottle and the system can be provided with a plurality of liquid storage bottles to provide required reagents for solid-phase extraction, and each liquid storage bottle is connected with one gating end corresponding to the selection valve.

The common end of the selection valve is connected with the outlet (inlet) of the syringe, and the common end of the selection valve can be communicated with only one gating end at a time; the gate terminal specific (designated) to the selector valve is connected to the common terminal of the distribution valve.

Each gating end of the distribution valve is correspondingly connected with a common end of each three-way valve in the first-stage three-way valve group one by one; and each normally open end (or normally closed end) of the three-way valve in the first-stage three-way valve group is correspondingly connected with each sample tube inlet one by one.

The outlet of each sample tube is correspondingly connected with the common end of each three-way valve in the second-stage three-way valve group one by one; the normally open end (or normally closed end) of each three-way valve in the second-stage three-way valve group is correspondingly connected with the normally open end (or normally closed end) of each three-way valve in the third-stage three-way valve group; the common end of each three-way valve in the third-stage three-way valve group is connected with the inlet of each buffer tube; the normally closed end (or normally open end) of each three-way valve in the third-stage three-way valve group is connected with the normally closed end (or normally open end) of each three-way valve in the first-stage three-way valve group.

The outlet of each buffer tube is connected with the inlet of each series extraction column of the second stage; if no buffer tube is required, the common end of each three-way valve in the third stage three-way valve set can be directly connected to the inlet of each second stage series extraction column.

The outlet of each series extraction column of the second stage is connected with a sample collecting unit; the collecting unit is used for collecting target components of solid phase extraction.

The sample tube and the buffer tube are respectively arranged on respective support frames, and each support frame can be driven by a motor to turn over by 180 degrees up and down.

The three units of the selection valve group, the distribution valve group and the injection pump can be expanded into a plurality of selection valves, distribution valves and injection pumps so as to increase the number of channels for processing samples in parallel; taking the expansion number of 2 as an example, 2 selector valves, 2 distribution valves and 2-way injectors are correspondingly needed; wherein the same gating end of each selection valve and the distribution valve is in a parallel operation state, namely 2 sample channels can operate in parallel.

The number of specific valves in the selection valve group and the distribution valve group can be increased according to the same number, and a first-stage three-way valve group, a sample tube, a ground first-stage extraction column, a second-stage three-way valve group, a third-stage three-way valve group, a buffer tube, a second-stage extraction column and a collecting tube which are connected with the distribution valves are correspondingly increased according to the same proportion. Thereby increasing the flux of a single parallel processing sample of the series column solid phase extraction system and increasing the total amount of sample processing of the system.

The three units of the selection valve group, the distribution valve group and the injection pump can be expanded into a plurality of selection valves, distribution valves and injectors so as to increase the number of channels for processing samples in parallel; referring to fig. 3, taking the expansion number 2 as an example, 2 selector valves, 2 distribution valves, and 2-way syringe pumps are correspondingly required; wherein the same gating end of each selector valve and the same gating end of each distribution valve are in a parallel operation state, and a waste liquid barrel is adopted at the same time, so that 2 sample channels can operate simultaneously.

The invention is a single-stage (one-stage) solid-phase extraction system when the first-stage extraction column is directly connected with the collecting pipe.

The selection valve and the distribution valve are both motor-driven rotary cutting valves, such as multi-way switching valves with model number SV-06. The first and second open ends of all three-way valves cannot be communicated with each other and can only be respectively communicated with the public port.

The waste liquid barrel is connected with a gating end of the selection valve and is used under the following conditions: when the injector sucks one liquid and needs to switch to the next different liquid, the interference of the first liquid reagent to the second liquid reagent (the residual problems of pipelines and the inner wall of the injector) is prevented; the solution is as follows: firstly absorbing a part of required target liquid to rinse the inner wall of the pipeline and the injector, then rotatably cutting the pipeline to a waste liquid port, discharging the waste liquid to a waste liquid barrel, and finally rotatably cutting the target liquid port back to absorb the target liquid. (rinsing with the target solution to ensure as little disturbance of the former liquid as possible).