阅读说明：本技术 一种微生物盐碱土壤改良剂及其制备方法与应用 (Microbial saline-alkali soil conditioner and preparation method and application thereof ) 是由 吴元凤 孟庆伟 段雪雯 张少骅 江洋 马婷 柳慧静 申术霞 闫冲 王倩倩 于 2021-07-28 设计创作，主要内容包括：本发明公开了一种微生物盐碱土壤改良剂及其制备方法与应用。属于土壤改良技术领域。该改良剂包括载体及复合微生物；所述载体包括如下质量百分比的组分：凹凸棒粉10～15％、腐殖酸8～12％、余量为有机肥；所述复合微生物包括枯草芽孢杆菌、放线菌和胶冻样类芽孢杆菌。与现有技术相比,本发明取得的有益效果为：将本发明改良剂施用到待改良的盐碱土壤中,施用量为150kg/亩,施用一个作物的生长周期,可有效的降低土壤pH0.1～0.5个单位,降低土壤容重10％。(The invention discloses a microbial saline-alkali soil conditioner and a preparation method and application thereof. Belongs to the technical field of soil improvement. The modifier comprises a carrier and a compound microorganism; the carrier comprises the following components in percentage by mass: 10-15% of attapulgite powder, 8-12% of humic acid and the balance of organic fertilizer; the compound microorganism comprises bacillus subtilis, actinomycetes and paenibacillus mucilaginosus. Compared with the prior art, the invention has the following beneficial effects: the conditioner is applied to the saline-alkali soil to be improved, the application amount is 150 kg/mu, the pH of the soil can be effectively reduced by 0.1-0.5 unit by applying the conditioner to the growth cycle of one crop, and the volume weight of the soil is reduced by 10%.)

1. A microbial saline-alkali soil conditioner is characterized by comprising a carrier and a compound microorganism;

the carrier comprises the following components in percentage by mass: 10-15% of attapulgite powder, 8-12% of humic acid and the balance of organic fertilizer;

the compound microorganism comprises bacillus subtilis, actinomycetes and paenibacillus mucilaginosus.

2. The microbial saline-alkali soil amendment according to claim 1, which comprises a carrier and a compound microorganism;

the carrier comprises the following components in percentage by mass: 11.8% of attapulgite powder, 10% of humic acid and the balance of organic fertilizer;

the compound microorganism comprises bacillus subtilis, actinomycetes and paenibacillus mucilaginosus.

3. The microbial saline-alkali soil improver as claimed in any one of claims 1 or 2, wherein the microbial saline-alkali soil improver has an effective viable count of not less than 3.0 hundred million/g, an organic matter content of not less than 50%, a pH of 7.5 to 9.0, and a water content of not less than 30%.

4. The microbial amendment for saline-alkali soil according to claim 1 or 2, wherein the bacillus subtilis has a bacterial content ≧ 1 × 10¹⁰Hundred million/ml, the bacteria content of the actinomycetes is not less than 1 multiplied by 10⁹Hundred million/ml, the bacteria content of the paenibacillus mucilaginosus is not less than 1 multiplied by 10¹⁰Billion/g;

wherein the addition amount of each microbial inoculum in the soil conditioner is 10-20 ml, 50-100 ml and 5-10 g per 1000 g.

5. A microbial amendment according to claim 1 or 2, wherein the organic fertilizer is in accordance with the NY/T525-2021.

6. A method for preparing a microbial saline-alkali soil amendment according to any one of claims 1 or 2, which comprises the following steps:

(1) preparation of the carrier: weighing attapulgite powder, humic acid and organic fertilizer according to the mass percentage, and uniformly mixing for later use;

(2) preparing a compound microorganism:

(21) respectively inoculating bacillus subtilis and actinomycetes to corresponding culture media, stirring and fermenting to obtain fermentation liquor, wherein the fermentation temperature is 32-35 ℃, the fermentation time is 48 +/-12 hours, and the stirring speed is 200 r/min;

(22) centrifuging the fermentation liquor, taking the supernatant, and concentrating to a required concentration to obtain a concentrated solution;

(3) and uniformly mixing the concentrated solution, the paenibacillus mucilaginosus and the carrier.

7. The preparation method according to claim 6, wherein the Bacillus subtilis culture medium in the step (21) comprises the following components in parts by mass: 20-30 parts of glucose, 15-25 parts of corn flour and soybean meal and MgSO (MgSO)₄0.05 to 0.08 portion of (NH)₄)₂SO₄0.3 to 0.5 part of K₂HPO₄0.5-1.0 parts of water 800 &1200 parts of (B); the volume ratio of the bacillus subtilis seed liquid to the bacillus subtilis culture medium is 1-1.5%;

the actinomycete culture medium comprises the following components in parts by weight: 1.0-1.2 parts of potassium nitrate, 0.4-0.8 part of dipotassium hydrogen phosphate, 0.5-0.9 part of magnesium sulfate, 0.5-1.0 part of sodium chloride, 15-20 parts of soluble starch and 800-1200 parts of water; the volume ratio of the actinomycete seed liquid to the actinomycete culture medium is 2-3%.

8. The method according to claim 6, wherein the centrifugation in the step (22) is carried out at 4000r/min for 5 min.

9. The method according to claim 6, wherein in step (22), the fermentation broth of Bacillus subtilis is concentrated to a cell content of 1X 10 ≧ 1¹⁰Billion/ml, concentrating actinomycete fermentation liquor to the bacteria content ≧ 1 × 10⁹Billion/ml.

10. The use of a microbial saline alkali soil amendment according to any one of claims 1 or 2 for soil improvement at an application rate of 150 kg/acre.

Technical Field

The invention relates to the technical field of soil improvement, in particular to a microbial saline-alkali soil conditioner and a preparation method and application thereof.

Background

The saline-alkali soil comprises: primary salinized, secondary salinized and various alkalized soils. The saline-alkali soil is clean soil which is not polluted by chemical fertilizers and pesticides, and the heavy metal content is low, so that the saline-alkali soil is defined as a reserve cultivated land resource, namely a cultivated land strategic reserve resource, and the method has great significance for guaranteeing national food safety and ecological safety. Under the actual condition that the resources of the back-up ploughed land are deficient, the practice of developing the directional cultivation, maintenance and tending process of the saline-alkali land (back-up ploughed land) to the ploughed land is very important.

Regarding the improvement of saline-alkali soil, the whole process goes through four stages of drainage improvement technology stage and technology application stage adopting biological measure to improve, improvement stage combining biological technology and engineering technology, and comprehensive improvement technology stage. A plurality of researchers at home and abroad research the formation and distribution of saline-alkali soil and the occurrence characteristics of salinization, and through continuous progress and development, the research is carried out to the stage of comprehensively treating the saline-alkali soil, and a new step is formed for the improvement and treatment of the saline-alkali soil. Although the saline-alkali soil is improved by using the microbial improver at the test stage, most of the improver has poor improvement effect and long application period, and generally needs 2-3 years, the method has higher practicability for farmers and needs to be studied deeply.

In conclusion, how to provide a microbial saline-alkali soil conditioner is a problem which needs to be solved urgently by the technical personnel in the field.

Disclosure of Invention

In view of the above, the invention provides a microbial saline-alkali soil conditioner, and a preparation method and application thereof. The invention utilizes the biological organic fertilizer to improve the saline-alkali soil, explores a practical, cheap and suitable method and material for effectively improving the saline-alkali soil, realizes the improvement and the effective utilization of the saline-alkali soil according to the principle of efficiently utilizing waste resources and reducing environmental pollution, enables the saline-alkali soil to become an agricultural available resource, promotes the sustainable development of society, agriculture and economy, and has important promotion significance for the benefit of the resources.

In order to achieve the purpose, the invention adopts the following technical scheme:

a microbial saline-alkali soil conditioner comprises a carrier and a compound microorganism;

the carrier comprises the following components in percentage by mass: 10-15% of attapulgite powder, 8-12% of humic acid and the balance of organic fertilizer;

the compound microorganism comprises bacillus subtilis, actinomycetes and paenibacillus mucilaginosus.

The beneficial effects are as follows: the attapulgite powder and the humic acid are selected, so that the safety is high, various mineral substances can be effectively released, the soil alkalinity can be effectively improved, the physicochemical property of the saline-alkali soil is improved, the loss of soil moisture is reduced, and the sustainable development of the saline-alkali soil is ensured.

The addition of bacillus subtilis and actinomycetes can affect the microbial community structure, the number and the diversity of bacteria in the saline-alkali soil, and the addition of the paenibacillus mucilaginosus can effectively improve the contents of soil organic matters, quick-acting P, quick-acting K and alkaline hydrolysis N, reduce the pH of the soil, and remove free Na in the saline-alkali soil⁺Taking away the fertilizer, and fundamentally improving the saline-alkali soil.

Further, a microbial saline-alkali soil conditioner comprises a carrier and compound microorganisms;

the carrier comprises the following components in percentage by mass: 11.8% of attapulgite powder, 10% of humic acid and the balance of organic fertilizer;

the compound microorganism comprises bacillus subtilis, actinomycetes and paenibacillus mucilaginosus.

Furthermore, the number of effective viable bacteria of the microbial saline-alkali soil conditioner is not less than 3.0 hundred million/g, the organic matter is not less than 50%, the pH value is 7.5-9.0, and the water content is not less than 30%.

Further, the bacterium content of the bacillus subtilis is ≧ 1 × 10¹⁰Hundred million/ml, the bacteria content of the actinomycetes is not less than 1 multiplied by 10⁹Hundred million/ml, the bacteria content of the paenibacillus mucilaginosus is not less than 1 multiplied by 10¹⁰Billion/g;

wherein the addition amount of each microbial inoculum in the soil conditioner is 10-20 ml, 50-100 ml and 5-10 g per 1000 g.

Furthermore, the main components of the organic fertilizer are sheep manure and crop straws, and the indexes meet the regulations of NY/T525-2021.

A preparation method of a microbial saline-alkali soil conditioner comprises the following steps:

(1) preparation of the carrier: weighing attapulgite powder, humic acid and organic fertilizer according to the mass percentage, and uniformly mixing for later use;

(2) preparing a compound microorganism:

(21) respectively inoculating bacillus subtilis and actinomycetes to corresponding culture media, stirring and fermenting to obtain fermentation liquor, wherein the fermentation temperature is 32-35 ℃, the fermentation time is 48 +/-12 hours, and the stirring speed is 200 r/min;

in the fermentation process, stirring is not required all the time, and the stirring can be stopped for 0.5-1 h every 4-6 h. In the fermentation process, the intermittent stirring process is relatively continuous, and the bacteria content can be effectively improved by 1-1.5%.

(22) Centrifuging the fermentation liquor, taking the supernatant, and concentrating to a required concentration to obtain a concentrated solution;

(3) and uniformly mixing the concentrated solution, the paenibacillus mucilaginosus and the carrier.

Further, the bacillus subtilis culture medium in the step (21) comprises the following components in parts by mass: 20-30 parts of glucose, 15-25 parts of corn flour and soybean meal and MgSO (MgSO)₄0.05 to 0.08 portion of (NH)₄)₂SO₄0.3 to 0.5 part of K₂HPO₄0.5-1.0 part of water and 800-1200 parts of water; the volume ratio of the bacillus subtilis seed liquid to the bacillus subtilis culture medium is 1-1.5%;

the actinomycete culture medium comprises the following components in parts by weight: 1.0-1.2 parts of potassium nitrate, 0.4-0.8 part of dipotassium hydrogen phosphate, 0.5-0.9 part of magnesium sulfate, 0.5-1.0 part of sodium chloride, 15-20 parts of soluble starch and 800-1200 parts of water; the volume ratio of the actinomycete seed liquid to the actinomycete culture medium is 2-3%.

Further, the centrifugation condition in the step (22) is 4000r/min for 5 min.

Further, in the step (22), the bacillus subtilis fermentation liquor is concentrated to a bacteria content ≧ 1 × 10¹⁰Billion/ml, concentrating actinomycete fermentation liquor to the bacteria content ≧ 1 × 10⁹Billion/ml.

Further, the application of the microbial saline-alkali soil conditioner in soil improvement is characterized in that the application amount is 150 kg/mu.

According to the technical scheme, compared with the prior art, the invention has the following beneficial effects: (1) the humic acid and the attapulgite powder can effectively improve the alkalinity of the soil, improve the physical and chemical properties of the soil and reduce the loss of soil moisture; the bacillus subtilis and the actinomycetes can promote the saline alkali improvement of soil and promote the decomposition of related antibiotic substances, and have no inhibiting effect on other bacteria; the addition of the paenibacillus mucilaginosus can effectively increase the water retention of the saline-alkali soil and reduce the loss of water. (2) The prepared modifier is compounded with the specification of NY884-2012, and the quality guarantee period is not less than 6 months. (3) The conditioner is applied to the saline-alkali soil to be improved, the pH value of the soil can be effectively reduced by 0.1-0.5 unit by applying the conditioner in the growth period of one crop (about 1 year), the volume weight of the soil is reduced by 10%, the microbial diversity in the soil is improved, and the number of microbial flora in the soil is enriched.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

The agents required for the embodiments of the present invention are conventional experimental agents purchased from commercially available sources, such as:

paenibacillus mucilaginosus is purchased from a Schchen Sipex manufacturer;

the experimental methods not mentioned in the examples are conventional experimental methods, and are not described in detail herein.

Example 1

A microbial saline-alkali soil conditioner comprises a carrier and a compound microorganism;

the carrier comprises the following components in percentage by mass: 10% of attapulgite powder, 8% of humic acid and 82% of organic fertilizer;

the compound microorganism comprises Bacillus subtilis, Actinomyces and Paenibacillus mucilaginosus.

The number of effective viable bacteria of the microbial saline-alkali soil conditioner is not less than 3.0 hundred million/g, the organic matter is not less than 50%, the pH is 7.5, and the water content is not less than 30%.

The bacteria content of Bacillus subtilis is ≧ 1 × 10¹⁰Hundred million/ml, actinomycetes containing ≧ 1 × 10⁹Hundred million/ml, and the bacterial content of the bacillus mucilaginosus is not less than 1 multiplied by 10¹⁰Billion/g.

The indexes of the organic fertilizer meet the regulations of NY/T525-2021.

The preparation method comprises the following steps:

(1) preparation of the carrier: weighing attapulgite powder, humic acid and organic fertilizer according to the mass percentage, and uniformly mixing for later use;

(2) preparing a compound microorganism:

(21) respectively inoculating bacillus subtilis and actinomycetes to corresponding culture media, stirring and fermenting to obtain fermentation liquor, wherein the fermentation temperature is 32 ℃, the fermentation time is 36 hours, and the stirring speed is 200 r/min;

the bacillus subtilis culture medium: 20kg of glucose, 15kg of corn flour and soybean meal and MgSO₄0.05kg、(NH₄)₂SO₄0.3kg、K₂HPO₄0.5kg and 800kg of water; the volume ratio of the bacillus subtilis seed liquid to the bacillus subtilis culture medium is 1 percent;

actinomycetes culture medium: 1.0kg of potassium nitrate, 0.4kg of dipotassium hydrogen phosphate, 0.5kg of magnesium sulfate, 0.5kg of sodium chloride, 15kg of soluble starch and 800kg of water; the volume ratio of the actinomycete seed liquid to the actinomycete culture medium is 2%.

(22) Centrifuging the fermentation liquor, taking the supernatant, and concentrating to a required concentration to obtain a concentrated solution;

centrifuging for 5min at 4000 r/min;

concentrating the Bacillus subtilis fermentation liquid to a bacteria content ≧ 1 × 10¹⁰Billion/ml, concentrating actinomycete fermentation liquor to the bacteria content ≧ 1 × 10⁹Billion/ml.

(3) And (3) uniformly mixing the concentrated solution, the paenibacillus mucilaginosus and the carrier (wherein the concentrated solution is added in a mode of air drying for 48 hours under an aseptic condition according to a proportion, and the paenibacillus mucilaginosus is directly added into a commercially available microbial inoculum).

Example 2

A microbial saline-alkali soil conditioner comprises a carrier and a compound microorganism;

the carrier comprises the following components in percentage by mass: 15% of attapulgite powder, 12% of humic acid and 73% of organic fertilizer;

the compound microorganism comprises Bacillus subtilis, Actinomyces and Paenibacillus mucilaginosus.

The number of effective viable bacteria of the microbial saline-alkali soil conditioner is not less than 3.0 hundred million/g, the organic matter is not less than 50%, the pH is 9.0, and the water content is not less than 30%.

The bacteria content of Bacillus subtilis is ≧ 1 × 10¹⁰Hundred million/ml, actinomycetes containing ≧ 1 × 10⁹Hundred million/ml, and the bacterial content of the bacillus mucilaginosus is not less than 1 multiplied by 10¹⁰Billion g.

The indexes of the organic fertilizer meet the regulations of NY/T525-2021.

The preparation method comprises the following steps:

(1) preparation of the carrier: weighing attapulgite powder, humic acid and organic fertilizer according to the mass percentage, and uniformly mixing for later use;

(2) preparing a compound microorganism:

(21) respectively inoculating bacillus subtilis and actinomycetes to corresponding culture media, stirring and fermenting to obtain fermentation liquor, wherein the fermentation temperature is 35 ℃, the fermentation time is 60 hours, and the stirring speed is 200 r/min;

the bacillus subtilis culture medium: 30kg of glucose, 25kg of corn flour and soybean meal and MgSO₄0.08kg、(NH₄)₂SO₄0.5kg、K₂HPO₄1.0kg and 1200kg of water; the volume ratio of the bacillus subtilis seed liquid to the bacillus subtilis culture medium is 1.5 percent;

actinomycetes culture medium: 1.2kg of potassium nitrate, 0.8kg of dipotassium hydrogen phosphate, 0.9kg of magnesium sulfate, 1.0kg of sodium chloride, 20kg of soluble starch and 1200kg of water; the volume ratio of the actinomycete seed liquid to the actinomycete culture medium is 3%.

(22) Centrifuging the fermentation liquor, taking the supernatant, and concentrating to a required concentration to obtain a concentrated solution;

centrifuging for 5min at 4000 r/min;

concentrating the Bacillus subtilis fermentation liquid to a bacteria content ≧ 1 × 10¹⁰Billion/ml, concentrating actinomycete fermentation liquor to the bacteria content ≧ 1 × 10⁹Billion/ml.

(3) And (3) uniformly mixing the concentrated solution, the paenibacillus mucilaginosus and the carrier (wherein the concentrated solution is added in a mode of air drying for 48 hours under an aseptic condition according to a proportion, and the paenibacillus mucilaginosus is directly added into a commercially available microbial inoculum).

Example 3

A microbial saline-alkali soil conditioner comprises a carrier and a compound microorganism;

the carrier comprises the following components in percentage by mass: 11.8% of attapulgite powder, 10% of humic acid and 78.2% of organic fertilizer;

the compound microorganism comprises Bacillus subtilis, Actinomyces and Paenibacillus mucilaginosus.

The number of effective viable bacteria of the microbial saline-alkali soil conditioner is not less than 3.0 hundred million/g, the organic matter is not less than 50%, the pH is 8.0, and the water content is not less than 30%.

The bacteria content of Bacillus subtilis is ≧ 1 × 10¹⁰Hundred million/ml, actinomycetes containing ≧ 1 × 10⁹Hundred million/ml, and the bacterial content of the bacillus mucilaginosus is not less than 1 multiplied by 10¹⁰Billion/g.

The indexes of the organic fertilizer meet the regulations of NY/T525-2021.

The preparation method comprises the following steps:

(1) preparation of the carrier: weighing attapulgite powder, humic acid and organic fertilizer according to the mass percentage, and uniformly mixing for later use;

(2) preparing a compound microorganism:

(21) respectively inoculating bacillus subtilis and actinomycetes to corresponding culture media, stirring and fermenting to obtain fermentation liquor, wherein the fermentation temperature is 33 ℃, the fermentation time is 48 hours, and the stirring speed is 200 r/min;

the bacillus subtilis culture medium: 25kg of glucose, 20kg of corn flour and soybean meal and MgSO₄0.06kg、(NH₄)₂SO₄0.4kg、K₂HPO₄0.7kg and 1000kg of water; the volume ratio of the bacillus subtilis seed liquid to the bacillus subtilis culture medium is 1.2 percent;

actinomycetes culture medium: 1.1kg of potassium nitrate, 0.6kg of dipotassium hydrogen phosphate, 0.7kg of magnesium sulfate, 0.7kg of sodium chloride, 17kg of soluble starch and 1000kg of water; the volume ratio of the actinomycete seed liquid to the actinomycete culture medium is 2.5%.

(22) Centrifuging the fermentation liquor, taking the supernatant, and concentrating to a required concentration to obtain a concentrated solution;

centrifuging for 5min at 4000 r/min;

concentrating the Bacillus subtilis fermentation liquid to a bacteria content ≧ 1 × 10¹⁰Billion/ml, concentrating actinomycete fermentation liquor to the bacteria content ≧ 1 × 10⁹Billion/ml.

(3) And (3) uniformly mixing the concentrated solution, the paenibacillus mucilaginosus and the carrier (wherein the concentrated solution is added in a mode of air drying for 48 hours under an aseptic condition according to a proportion, and the paenibacillus mucilaginosus is directly added into a commercially available microbial inoculum).

Application cases:

the test site is located on a northeast farm in east China of Shandong, and the soil type of the region is tidal soil. The test design is a positioning test of corn-wheat crop rotation, and the test years are 2015 and 2016. The soil texture of the test soil is sandy loam. The tested crops are semen Maydis (Zhengdan 958) and winter wheat (green wheat No. 6), and are completely randomized block design, each treatment is repeated for 3 times, and each cell area is 50m²(5m × l0m), and the total area is 1.5 mu.

The experimental plot is provided with 4 treatments, three times of repetition and 50m of cell area²And randomly arranging, and setting a protection row for 2 meters in a cell. The specific treatment is as follows:

(1) the treatment 1(K0) is used for the fertilization of farmers, the fertilization of corn in season is generally 16 kg of nitrogenous fertilizer, 8kg of phosphate fertilizer and 7kg of potash fertilizer applied per mu, and the fertilizers are spread and turned over. In wheat season, 18 kg of nitrogenous fertilizer, 10 kg of phosphate fertilizer and 5kg of potash fertilizer are applied per mu (pure), and the fertilizers are spread for ploughing.

(2) Treatment 2(K1) is optimized fertilization treatment, 12 kilograms of nitrogenous fertilizer, 4 kilograms of phosphate fertilizer and potash fertilizer are applied to each mu of corn in season, and the fertilizers are spread and ploughed. 16 kg of nitrogenous fertilizer, 8kg of phosphate fertilizer and 4kg of potash fertilizer are applied in wheat seasons (pure), and the fertilizers are spread for ploughing.

(3) And the treatment 3(K2) is respectively applied with the microbial soil conditioner which is inactivated at high temperature and high pressure and has the same quantity as the treatment 4 on the basis of the optimized fertilization of the two-season crops, and is applied with the fertilizer for ploughing.

(4) Treatment 4(K3) microbial soil conditioner (prepared in example 3) was basal applied at 150 kg/mu on the basis of optimum fertilization of the two-season crop, respectively, and was spread together with the fertilizer for tillage.

Soil sample determination after crops are harvested in each season, sampling is carried out on 5 plum blossom-shaped points in each cell, and soil is taken from the ground surface by a cutting ring at the depth of 0-20 cm. And (4) measuring the pH value of the soil and the volume weight of the soil before and after application.

The soil pH results are shown in table 1.

TABLE 1 soil pH before and after application

The results of the two-season positioning test show that: compared with the pH 8.4 of a basic soil sample, the change of the pH value of the soil treated by the method 1 is small, the pH values of the soil treated by the methods 2, 3 and 4 are reduced, and after the corn is harvested, the change of the pH value of the soil between the treatments reaches the level of difference significance; after wheat harvest, a significance level of 5% was achieved between treatment 3 and treatment 4. In general, soil conditioners can reduce the pH value of soil, and the pH value of soil can be continuously improved along with the increase of the service life.

The pH value of the soil is obviously reduced by the additional application of the soil conditioner 3 and the treatment 4, wherein the pH value of the soil is reduced most obviously by 0.27 compared with the conventional application (treatment 1) after the treatment 4; compared with treatment 3, the two-season test achieves the significance level of 5%, which shows that the microbial strains in the soil conditioner have the effect of improving the pH value of soil; in comparison with treatment 3, the first season test of treatment 2 reached a significance level of 5%, and the soil pH value in the second season tended to decrease, indicating that the carrier in the soil conditioner also had the effect of improving the soil pH value.

The soil volume weight results are shown in table 2.

TABLE 2 soil bulk weights before and after application

The volume weight of the soil is reduced along with the increasing application of the soil conditioner, and the difference of the volume weight of the soil between different treatments reaches the significance level of 5%. The volume weight of the soil is not greatly changed under the conditions of conventional fertilization (treatment 1) and optimized fertilization (treatment 2), and the total weight of the soil has an increasing trend; the soil volume weight reduction trend is obvious by additionally applying the soil conditioner 3 and the soil conditioner 4, wherein the soil volume weight reduction of the soil conditioner 4 is the most obvious and is reduced by 14.73 percent compared with the conventional fertilization (treatment 1); compared with treatment 3, the two-season test achieves the significance level of 5 percent, which shows that the microbial strains in the soil conditioner have the effect of improving the volume weight of soil; the two-season test also reached a significance level of 5% in treatment 2 compared to treatment 3, indicating that the carrier in the soil conditioner also has the effect of improving the volume weight of the soil.

The embodiments in the present description are described in a progressive manner, each embodiment focuses on differences from other embodiments, and the same and similar parts among the embodiments are referred to each other.

The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.