阅读说明：本技术 一种用于调节偏酸性土壤的生物调理剂及其制备方法和应用方法 (Biological conditioner for regulating subacid soil and preparation method and application method thereof ) 是由 阎应广 柏万文 黄亦 蒋天举 于 2021-08-03 设计创作，主要内容包括：本发明公开一种用于调节偏酸性土壤的生物调理剂及其制备方法和应用方法,属于土壤调节技术领域。本发明土壤调节剂是包括以下重量份的原料制备而成：甘蔗生物碳20-40份、高炉矿渣粉20-30份、腐植酸10-20份、沸石5-10份、石灰粉3-5份、微生物菌粉3-5份。本发明各原料相互作用协同配合,共同实现酸性土壤环境的理化性质调节,提升pH,增加土孔隙度,提升有机质等各类营养物质含量,提升土壤各类酶活性,同时实现了工业废渣的回收再利用,经济效益和社会效益显著。(The invention discloses a biological conditioner for regulating subacid soil as well as a preparation method and an application method thereof, belonging to the technical field of soil regulation. The soil conditioner is prepared from the following raw materials in parts by weight: 20-40 parts of sugarcane biochar, 20-30 parts of blast furnace slag powder, 10-20 parts of humic acid, 5-10 parts of zeolite, 3-5 parts of lime powder and 3-5 parts of microbial powder. The raw materials of the invention interact and cooperate with each other to jointly realize the adjustment of the physicochemical property of the acid soil environment, improve the pH, increase the soil porosity, improve the content of various nutrients such as organic matters and the like, improve the activity of various enzymes of the soil, realize the recycling of industrial waste residues, and have obvious economic benefit and social benefit.)

1. The biological conditioner for regulating the meta-acid soil is characterized by being prepared from the following raw materials in parts by weight: 20-40 parts of sugarcane biochar, 20-30 parts of blast furnace slag powder, 10-20 parts of humic acid, 5-10 parts of zeolite, 3-5 parts of lime powder and 3-5 parts of microbial powder.

2. The bioregulator for regulating subacid soils according to claim 1, wherein the sugar cane biochar is prepared by a method comprising: drying and pulverizing sugarcane peel, pre-carbonizing at 200 deg.C for 1-3 hr in muffle furnace, introducing nitrogen, and heating at 5 deg.C/min in nitrogen environment^-1Keeping the temperature at 500 ℃ for 1 h; at a temperature rise rate of 2 ℃ min^-1Keeping the temperature at 700 ℃ for 1h, and cooling to room temperature to obtain the sugarcane biochar.

3. The biological conditioner for conditioning meta-acid soil according to claim 1, wherein the blast furnace slag powder is prepared by the following method: ball-milling the blast furnace slag until the specific surface area is 300-²And/kg, adding a sodium hydroxide solution, soaking for 3-5h, drying, adding 3 wt% of an alkaline activator, mixing and ball-milling for 1-2h again to obtain the blast furnace slag powder.

4. The bioregulator for the regulation of meta-acid soils according to claim 3, wherein the alkaline activator is silica fume and calcium oxide 1: 1 and mixing.

5. The bioregulator for the conditioning of meta-acid soils according to claim 3, wherein the sodium hydroxide solution has a concentration of 1 mol/L.

6. The biological conditioner for regulating meta-acid soil according to claim 1, wherein the microbial powder is a mixture of bacillus subtilis powder and streptomyces albus powder, and the specific preparation method of the streptomyces albus powder comprises the following steps: inoculating Streptomyces albus preserved in a freeze-drying tube into a solid slant of a broth culture medium, and culturing at 30-32 ℃ for 24-48 hours; picking the bacterial colony to be inoculated into a sterilized liquid culture medium, and culturing for 24-36 hours at the temperature of 26-28 ℃ and under the condition of 150-; and (4) spray-drying the fermentation liquor to obtain the streptomyces albus powder.

7. The bioregulator for regulating meta-acid soil according to claim 6, wherein the liquid medium comprises the following components in parts by weight: 20 parts of sucrose, 5 parts of sodium chloride, 20 parts of peptone, 5 parts of magnesium sulfate, 3 parts of monopotassium phosphate and 1000 parts of water.

8. A method of preparing a bioregulator for the regulation of meta-acid soils as claimed in any one of claims 1 to 7 comprising the steps of:

(1) preparing the sugarcane biochar: drying and pulverizing sugarcane peel, pre-carbonizing at 200 deg.C for 1-3 hr in muffle furnace, introducing nitrogen, and heating at 5 deg.C/min in nitrogen environment^-1Keeping the temperature at 500 ℃ for 1 h; at a temperature rise rate of 2 ℃ min^-1Keeping the temperature at 700 ℃ for 1h, and cooling to room temperature to obtain the sugarcane biochar;

(2) preparing blast furnace slag powder: ball-milling the blast furnace slag until the specific surface area is 300-²Adding sodium hydroxide solution into the mixture to soak the mixture for 3 to 5 hours, drying the mixture, adding 3 weight percent of alkaline exciting agent into the mixture, mixing and ball-milling the mixture for 1 to 2 hours again to obtain blast furnace slag powder;

(3) mixing the raw materials according to the weight part, granulating by a granulator, controlling the particle size to be 3-6mm, fully drying after the granulation is finished, packaging and warehousing.

9. A method of using a bioregulator for the regulation of meta-acid soils according to any one of claims 1 to 7 wherein the conditioner is applied by broadcast, furrow or hole application or mixed with other fertilisers in the range of 20 to 30kg per acre.

Technical Field

The invention belongs to the technical field of soil regulation, and particularly relates to a biological conditioner for regulating subacid soil as well as a preparation method and an application method thereof.

Background

Soil acidification refers to a process in which a soil absorptive complex receives a certain amount of exchangeable hydrogen ions or aluminum ions to leach alkaline (base) ions in soil. The phenomenon of soil acidification is more and more serious due to large precipitation and strong concentrated leaching action in partial areas, large loss of alkaline salts such as calcium, magnesium, potassium and the like, acid rain generated by fire coal and industrial activities and large use of chemical nitrogen fertilizers in agricultural production.

Soil acidification affects the effectiveness of soil nutrients in the first place. After soil acidification, soil fertility is reduced, alkaline base ions in soil are reduced, and Al³⁺And H⁺And in the case of insufficient organic matters, the physical properties of the soil are deteriorated, the soil is heavily hardened, and the ventilation is poor, so that the quality of the soil is reduced. Research shows that the mineral dissolution, organic matter decomposition and soil nutrient element conversion of soil are related to the pH value of the soil, and Ca in the soil²⁺、Mg²⁺、K⁺The equal runoff quantity is in a positive correlation with the pH value of the soil, the content of soil nutrients is in a negative correlation with the pH value of the soil, the activity of metal ions in the soil can be enhanced after the soil is acidified, such as aluminum, iron, manganese, chromium and lead, the harm of heavy metals in the soil is aggravated, the fertilizer retention and supply capacity of the soil is low, the adsorption and fixation of the soil on phosphoric acid are influenced, and the fertilizer efficiency of a phosphate fertilizer is reduced. Furthermore, soil acidification can also affect soil enzyme activity. The pH value of the soil is increased, the activities of urease, phosphatase and protease are all inhibited, the activity of the soil enzyme is reduced, and finally soil microorganisms are affected and the harm of pathogenic microorganisms is aggravated.

The traditional method for improving the acid soil is to apply lime, the lime can obviously reduce the content of exchangeable acid and exchangeable aluminum in the soil, the acid reduction effect can be kept for a long time, but the application of a large amount of lime has the defects of soil hardening, calcification of available nutrients, reduction of soil fertilizer efficiency and the like, the long-term application of the lime is not favorable, the absorption and utilization of nutrient elements by crops are influenced, the frequent use also has a great negative effect on the soil micro-ecological environment, and the long-term sustainable development of agriculture is not favorable.

Therefore, how to reduce the use of lime, effectively improve the physicochemical property of the acid soil, improve the biological activity of the soil, and improve the yield and quality of crops is a problem to be solved urgently in the field of the current soil conditioner.

Disclosure of Invention

The invention provides a soil conditioner aiming at acid soil regulation, which reduces the negative influence of lime on soil on one hand, provides rich nutrient substances for soil on the other hand, regulates physical and chemical properties, improves the ecological activity of soil, and realizes the yield and income increase and the sustainable development of basic agriculture.

In order to achieve the technical purpose, the technical scheme adopted by the invention is as follows:

a biological conditioner for regulating meta-acid soil is prepared from the following raw materials in parts by weight: 20-40 parts of sugarcane biochar, 20-30 parts of blast furnace slag powder, 10-20 parts of humic acid, 5-10 parts of zeolite, 3-5 parts of lime powder and 3-5 parts of microbial powder.

Further, the preparation method of the sugarcane biochar comprises the following steps: drying and pulverizing sugarcane peel, pre-carbonizing at 200 deg.C for 1-3 hr in muffle furnace, introducing nitrogen, and heating at 5 deg.C/min in nitrogen environment^-1Keeping the temperature at 500 ℃ for 1 h; at a temperature rise rate of 2 ℃ min^-1Keeping the temperature at 700 ℃ for 1h, and cooling to room temperature to obtain the sugarcane biochar.

Further, the preparation method of the blast furnace slag powder comprises the following steps: ball-milling the blast furnace slag until the specific surface area is 300-²And/kg, adding a sodium hydroxide solution, soaking for 3-5h, drying, adding 3 wt% of an alkaline activator, mixing and ball-milling for 1-2h again to obtain the blast furnace slag powder.

Further, the alkali activator is silica fume and calcium oxide 1: 1 and mixing.

Further, the concentration of the sodium hydroxide solution is 1 mol/L.

The blast furnace slag is typical industrial waste and contains rich mineral elements, the slag is crushed under the action of mechanical force in the grinding process, the surface of particles generates defects and the breakage of network bonds, the network structure is favorably disintegrated, meanwhile, the surface of the slag particles is enlarged due to the reduction of the particle fineness, the activity of the slag is greatly improved under the action of an alkaline activator, the use amount of lime can be reduced to a certain degree, and the soil is activated.

Further, the microbial powder is a mixture of bacillus subtilis powder and streptomyces albus powder, and the specific preparation method of the streptomyces albus powder comprises the following steps: inoculating Streptomyces albus preserved in a freeze-drying tube into a solid slant of a broth culture medium, and culturing at 30-32 ℃ for 24-48 hours; picking the bacterial colony to be inoculated into a sterilized liquid culture medium, and culturing for 24-36 hours at the temperature of 26-28 ℃ and under the condition of 150-; and (4) spray-drying the fermentation liquor to obtain the streptomyces albus powder.

Further, the liquid culture medium comprises the following components in parts by weight: 20 parts of sucrose, 5 parts of sodium chloride, 20 parts of peptone, 5 parts of magnesium sulfate, 3 parts of monopotassium phosphate and 1000 parts of water.

The bacillus subtilis can be selected from commercial bacterial powder, and the Streptomyces albus strain (Streptomyces albus) is purchased from China general microbiological culture collection management center (address: China academy of sciences, 3, west road 1, north Chen, south China, Beijing), the collection number is CGMCC4.7658, and the collection date is 2019, 8 months and 23 days.

The viable count of the bacillus subtilis and the streptomyces albus in the microbial powder provided by the invention is respectively more than 30 hundred million/g and more than 1 hundred million/g.

A method for preparing a biological conditioner for conditioning meta-acid soil, comprising the following steps:

(1) preparing the sugarcane biochar: drying and pulverizing sugarcane peel, pre-carbonizing at 200 deg.C for 1-3 hr in muffle furnace, introducing nitrogen, and heating at 5 deg.C/min in nitrogen environment^-1Keeping the temperature at 500 ℃ for 1 h; at a temperature rise rate of 2 ℃ min^-1Keeping the temperature at 700 ℃ for 1h, and cooling to room temperature to obtain the sugarcane biochar;

(2) preparing blast furnace slag powder: ball milling blast furnace slagTo a specific surface area of 300-400m²Adding sodium hydroxide solution into the mixture to soak the mixture for 3 to 5 hours, drying the mixture, adding 3 weight percent of alkaline exciting agent into the mixture, mixing and ball-milling the mixture for 1 to 2 hours again to obtain blast furnace slag powder;

(3) mixing the raw materials according to the weight part, granulating by a granulator, controlling the particle size to be 3-6mm, fully drying after the granulation is finished, packaging and warehousing. Binders such as modified starch can be added during the granulation process to facilitate granulation.

A biological conditioner for regulating acid soil is prepared by applying the biological conditioner to the surface of acidic soil in field, ditch or hole, or mixing with other fertilizers, and the dosage per mu is 20-30 kg.

Advantageous effects

(1) According to the invention, the biomass charcoal is prepared by using the sugarcane waste through a pre-carbonization and stage heating calcination manner, the obtained biomass charcoal has a loose and porous structure, so that on one hand, a microbial agent is effectively protected to continuously play a role, on the other hand, blast furnace slag powder, lime powder and humic acid can be uniformly adsorbed, a continuous and efficient regulation effect on the soil acidic environment is achieved, the use of lime is reduced, meanwhile, nutrient substances in the humic acid are stably output, and the soil nutrient structure is optimized while the soil structure is regulated;

(2) the bacillus subtilis is a typical antibiotic agent, can effectively improve the disease resistance of crops, and the streptomyces albus can generate resistant substances such as lipopeptide, peptide, phospholipid, bacteriocin and the like, has good prevention and control effects on bacterial, fungal and even viral plant diseases, and can greatly improve the disease resistance of soil and improve the yield by matching the bacillus subtilis and the streptomyces albus; meanwhile, lipopeptide substances are good biosurfactants, and can promote the uniform distribution of fine particles such as blast furnace slag powder, lime powder and the like while promoting the function of resistant substances, so that the lipopeptide substances can continuously and effectively play a role in soil regulation;

(3) the raw materials of the invention interact and cooperate with each other to jointly realize the adjustment of the physicochemical property of the acid soil environment, improve the pH, increase the porosity, improve the content of various nutrients such as organic matters and the like, improve the activity of various enzymes of the soil, realize the recycling of industrial waste residues, and have obvious economic benefit and social benefit.

Drawings

FIG. 1 is a microscopic view of sugar cane biochar in example 3 of the present invention;

FIG. 2 is a partial enlarged view of the microstructure of the sugar cane biochar in example 3 of the present invention.

Detailed Description

The technical solution of the present invention is further described below with reference to specific embodiments, but is not limited thereto.

Example 1

A biological conditioner for regulating meta-acid soil is prepared from the following raw materials in parts by weight: 20 parts of sugarcane biochar, 20 parts of blast furnace slag powder, 10 parts of humic acid, 5 parts of zeolite, 3 parts of lime powder and 3 parts of microbial powder.

The preparation method of the sugarcane biochar comprises the following steps: drying and crushing sugarcane peel, pre-carbonizing the sugarcane peel in a muffle furnace for 1h at 200 ℃, introducing nitrogen, and heating at 5 ℃ for min in a nitrogen environment^-1Keeping the temperature at 500 ℃ for 1 h; at a temperature rise rate of 2 ℃ min^-1Keeping the temperature at 700 ℃ for 1h, and cooling to room temperature to obtain the sugarcane biochar.

The preparation method of the blast furnace slag powder comprises the following steps: ball-milling the blast furnace slag until the specific surface area is 300-²And/kg, adding a sodium hydroxide solution, soaking for 3 hours, drying, adding 3 wt% of an alkaline activator, mixing and ball-milling for 1 hour again to obtain the blast furnace slag powder.

The alkali activator is silica fume and calcium oxide 1: 1 and mixing.

The concentration of the sodium hydroxide solution is 1 mol/L.

The microbial powder is a mixture of bacillus subtilis powder and streptomyces albus powder, and the specific preparation method of the streptomyces albus powder comprises the following steps: inoculating Streptomyces albus preserved in a freeze-drying tube into a solid slant of a broth culture medium, and culturing at 30-32 ℃ for 24 hours; picking the bacterial colony to be inoculated into a sterilized liquid culture medium, and culturing for 24 hours at the temperature of 26-28 ℃ and under the condition of 150-; and (4) spray-drying the fermentation liquor to obtain the streptomyces albus powder.

The liquid culture medium comprises the following components in parts by weight: 20 parts of sucrose, 5 parts of sodium chloride, 20 parts of peptone, 5 parts of magnesium sulfate, 3 parts of monopotassium phosphate and 1000 parts of water.

The Bacillus subtilis can be commercially available bacterial powder, and Streptomyces albus strain (Streptomyces albus) is purchased from China general microbiological culture Collection center (address: China academy of sciences, China, No. 3, West Lu No. 1, Beijing, Chaoyang, and has a preservation number of CGMCC4.7658 and a preservation date of 2019, 8 months and 23 days.

The viable count of the bacillus subtilis and the streptomyces albus in the microbial powder is respectively more than 30 hundred million/g and more than 1 hundred million/g.

A method for preparing a biological conditioner for conditioning meta-acid soil, comprising the following steps:

(1) preparing the sugarcane biochar: drying and crushing sugarcane peel, pre-carbonizing the sugarcane peel in a muffle furnace for 1h at 200 ℃, introducing nitrogen, and heating at 5 ℃ for min in a nitrogen environment^-1Keeping the temperature at 500 ℃ for 1 h; at a temperature rise rate of 2 ℃ min^-1Keeping the temperature at 700 ℃ for 1h, and cooling to room temperature to obtain the sugarcane biochar;

(2) preparing blast furnace slag powder: ball-milling the blast furnace slag until the specific surface area is 300-²Adding sodium hydroxide solution into the mixture to soak the mixture for 3 hours, drying the mixture, adding 3 wt% of alkaline exciting agent into the mixture, mixing and ball-milling the mixture for 1 hour again to obtain blast furnace slag powder;

(3) mixing the raw materials according to the weight part, granulating by a granulator, controlling the particle size to be 3-6mm, fully drying after the granulation is finished, packaging and warehousing. Binders such as modified starch can be added during the granulation process to facilitate granulation.

A biological conditioner for regulating acid soil is prepared by applying the biological conditioner to the surface of acidic soil in field, ditch or hole, or mixing with other fertilizers, and the dosage per mu is 20-30 kg.

Example 2

A biological conditioner for regulating meta-acid soil is prepared from the following raw materials in parts by weight: 30 parts of sugarcane biochar, 20 parts of blast furnace slag powder, 15 parts of humic acid, 5 parts of zeolite, 3 parts of lime powder and 3 parts of microbial powder.

The preparation method of the sugarcane biochar comprises the following steps: drying and crushing sugarcane peel, pre-carbonizing the sugarcane peel in a muffle furnace for 3 hours at 200 ℃, introducing nitrogen, and heating at 5 ℃ for min in a nitrogen environment^-1Keeping the temperature at 500 ℃ for 1 h; at a temperature rise rate of 2 ℃ min^-1Keeping the temperature at 700 ℃ for 1h, and cooling to room temperature to obtain the sugarcane biochar.

The preparation method of the blast furnace slag powder comprises the following steps: ball-milling the blast furnace slag until the specific surface area is 300-²And/kg, adding a sodium hydroxide solution, soaking for 5 hours, drying, adding 3 wt% of an alkaline activator, mixing and ball-milling for 2 hours again to obtain blast furnace slag powder.

The alkali activator is silica fume and calcium oxide 1: 1 and mixing.

The concentration of the sodium hydroxide solution is 1 mol/L.

The microbial powder is a mixture of bacillus subtilis powder and streptomyces albus powder, and the specific preparation method of the streptomyces albus powder comprises the following steps: inoculating Streptomyces albus preserved in a freeze-drying tube into a solid slant of a broth culture medium, and culturing at 30-32 ℃ for 48 hours; picking the bacterial colony to be inoculated into a sterilized liquid culture medium, and culturing for 36 hours at the temperature of 26-28 ℃ and under the condition of 150-; and (4) spray-drying the fermentation liquor to obtain the streptomyces albus powder.

The liquid culture medium comprises the following components in parts by weight: 20 parts of sucrose, 5 parts of sodium chloride, 20 parts of peptone, 5 parts of magnesium sulfate, 3 parts of monopotassium phosphate and 1000 parts of water.

The Bacillus subtilis can be commercially available bacterial powder, and Streptomyces albus strain (Streptomyces albus) is purchased from China general microbiological culture Collection center (address: China academy of sciences, China, No. 3, West Lu No. 1, Beijing, Chaoyang, and has a preservation number of CGMCC4.7658 and a preservation date of 2019, 8 months and 23 days.

The viable count of the bacillus subtilis and the streptomyces albus in the microbial powder is respectively more than 30 hundred million/g and more than 1 hundred million/g.

A method for preparing a biological conditioner for conditioning meta-acid soil, comprising the following steps:

(1) preparing the sugarcane biochar: drying and crushing sugarcane peel, pre-carbonizing the sugarcane peel in a muffle furnace for 3 hours at 200 ℃, introducing nitrogen, and heating at 5 ℃ for min in a nitrogen environment^-1Keeping the temperature at 500 ℃ for 1 h; at a temperature rise rate of 2 ℃ min^-1Keeping the temperature at 700 ℃ for 1h, and cooling to room temperature to obtain the sugarcane biochar;

(2) preparing blast furnace slag powder: ball-milling the blast furnace slag until the specific surface area is 300-²Adding sodium hydroxide solution to soak for 5 hours, drying, adding 3 wt% of alkaline activator, mixing and ball-milling for 2 hours again to obtain blast furnace slag powder;

(3) mixing the raw materials according to the weight part, granulating by a granulator, controlling the particle size to be 3-6mm, fully drying after the granulation is finished, packaging and warehousing. Binders such as modified starch can be added during the granulation process to facilitate granulation.

A biological conditioner for regulating acid soil is prepared by applying the biological conditioner to the surface of acidic soil in field, ditch or hole, or mixing with other fertilizers, and the dosage per mu is 20-30 kg.

Example 3

A biological conditioner for regulating meta-acid soil is prepared from the following raw materials in parts by weight: 40 parts of sugarcane biochar, 30 parts of blast furnace slag powder, 20 parts of humic acid, 10 parts of zeolite, 5 parts of lime powder and 5 parts of microbial powder.

The preparation method of the sugarcane biochar comprises the following steps: drying and crushing sugarcane peel, pre-carbonizing the sugarcane peel in a muffle furnace for 3 hours at 200 ℃, introducing nitrogen, and heating at 5 ℃ for min in a nitrogen environment^-1Keeping the temperature at 500 ℃ for 1 h; at a temperature rise rate of 2 ℃ min^-1Keeping the temperature at 700 ℃ for 1h, and cooling to room temperature to obtain the sugarcane biochar.

The microstructure diagram of the obtained sugarcane biochar is shown in figure 1, and the diagram shows that the sugarcane biochar prepared by the invention has a good honeycomb structure, can effectively adsorb raw material substances, can effectively play a role in supplying and regulating, and simultaneously plays a role in protecting microorganisms.

The preparation method of the blast furnace slag powder comprises the following steps: ball-milling the blast furnace slag until the specific surface area is 300-²And/kg, adding a sodium hydroxide solution, soaking for 5 hours, drying, adding 3 wt% of an alkaline activator, mixing and ball-milling for 2 hours again to obtain blast furnace slag powder.

The alkali activator is silica fume and calcium oxide 1: 1 and mixing.

The concentration of the sodium hydroxide solution is 1 mol/L.

The blast furnace slag is typical industrial waste and contains rich mineral elements, the slag is crushed under the action of mechanical force in the grinding process, the surface of particles generates defects and the breakage of network bonds, the network structure is favorably disintegrated, meanwhile, the surface of the slag particles is enlarged due to the reduction of the particle fineness, the activity of the slag is greatly improved under the action of an alkaline activator, the use amount of lime can be reduced to a certain degree, and the soil is activated.

The microbial powder is a mixture of bacillus subtilis powder and streptomyces albus powder, and the specific preparation method of the streptomyces albus powder comprises the following steps: inoculating Streptomyces albus preserved in a freeze-drying tube into a solid slant of a broth culture medium, and culturing at 30-32 ℃ for 48 hours; picking the bacterial colony to be inoculated into a sterilized liquid culture medium, and culturing for 36 hours under the conditions of 28 ℃, 150-; and (4) spray-drying the fermentation liquor to obtain the streptomyces albus powder.

The liquid culture medium comprises the following components in parts by weight: 20 parts of sucrose, 5 parts of sodium chloride, 20 parts of peptone, 5 parts of magnesium sulfate, 3 parts of monopotassium phosphate and 1000 parts of water.

The Bacillus subtilis can be commercially available bacterial powder, and Streptomyces albus strain (Streptomyces albus) is purchased from China general microbiological culture Collection center (address: China academy of sciences, China, No. 3, West Lu No. 1, Beijing, Chaoyang, and has a preservation number of CGMCC4.7658 and a preservation date of 2019, 8 months and 23 days.

The viable count of the bacillus subtilis and the streptomyces albus in the microbial powder is respectively more than 30 hundred million/g and more than 1 hundred million/g.

A method for preparing a biological conditioner for conditioning meta-acid soil, comprising the following steps:

(1) preparing the sugarcane biochar: drying and crushing sugarcane peel, pre-carbonizing the sugarcane peel in a muffle furnace for 3 hours at 200 ℃, introducing nitrogen, and heating at 5 ℃ for min in a nitrogen environment^-1Keeping the temperature at 500 ℃ for 1 h; at a temperature rise rate of 2 ℃ min^-1Keeping the temperature at 700 ℃ for 1h, and cooling to room temperature to obtain the sugarcane biochar;

(2) preparing blast furnace slag powder: ball-milling the blast furnace slag until the specific surface area is 300-²Adding sodium hydroxide solution to soak for 5 hours, drying, adding 3 wt% of alkaline activator, mixing and ball-milling for 2 hours again to obtain blast furnace slag powder;

(3) mixing the raw materials according to the weight part, granulating by a granulator, controlling the particle size to be 3-6mm, fully drying after the granulation is finished, packaging and warehousing. Binders such as modified starch can be added during the granulation process to facilitate granulation.

A biological conditioner for regulating acid soil is prepared by applying the biological conditioner to the surface of acidic soil in field, ditch or hole, or mixing with other fertilizers, and the dosage per mu is 20-30 kg.

Comparative example 1

A biological conditioner for regulating meta-acid soil is prepared from the following raw materials in parts by weight: 40 parts of biological carbon, 30 parts of blast furnace slag powder, 20 parts of humic acid, 10 parts of zeolite, 5 parts of lime powder and 5 parts of microbial powder.

In the comparative example, the sugarcane biochar is replaced by commercial biochar on the basis of example 3.

The preparation method of the blast furnace slag powder comprises the following steps: ball-milling the blast furnace slag until the specific surface area is 300-²And/kg, adding a sodium hydroxide solution, soaking for 5 hours, drying, adding 3 wt% of an alkaline activator, mixing and ball-milling for 2 hours again to obtain blast furnace slag powder.

The alkali activator is silica fume and calcium oxide 1: 1 and mixing.

The concentration of the sodium hydroxide solution is 1 mol/L.

The microbial powder is a mixture of bacillus subtilis powder and streptomyces albus powder, and the specific preparation method of the streptomyces albus powder comprises the following steps: inoculating Streptomyces albus preserved in a freeze-drying tube into a solid slant of a broth culture medium, and culturing at 30-32 ℃ for 48 hours; picking the bacterial colony to be inoculated into a sterilized liquid culture medium, and culturing for 36 hours under the conditions of 28 ℃, 150-; and (4) spray-drying the fermentation liquor to obtain the streptomyces albus powder.

The liquid culture medium comprises the following components in parts by weight: 20 parts of sucrose, 5 parts of sodium chloride, 20 parts of peptone, 5 parts of magnesium sulfate, 3 parts of monopotassium phosphate and 1000 parts of water.

The Bacillus subtilis can be commercially available bacterial powder, and Streptomyces albus strain (Streptomyces albus) is purchased from China general microbiological culture Collection center (address: China academy of sciences, China, No. 3, West Lu No. 1, Beijing, Chaoyang, and has a preservation number of CGMCC4.7658 and a preservation date of 2019, 8 months and 23 days.

The viable count of the bacillus subtilis and the streptomyces albus in the microbial powder is respectively more than 30 hundred million/g and more than 1 hundred million/g.

A method for preparing a biological conditioner for conditioning meta-acid soil, comprising the following steps:

(1) preparing blast furnace slag powder: ball-milling the blast furnace slag until the specific surface area is 300-²Adding sodium hydroxide solution to soak for 5 hours, drying, adding 3 wt% of alkaline activator, mixing and ball-milling for 2 hours again to obtain blast furnace slag powder;

(2) mixing the raw materials according to the weight part, granulating by a granulator, controlling the particle size to be 3-6mm, fully drying after the granulation is finished, packaging and warehousing. Binders such as modified starch can be added during the granulation process to facilitate granulation.

A biological conditioner for regulating acid soil is prepared by applying the biological conditioner to the surface of acidic soil in field, ditch or hole, or mixing with other fertilizers, and the dosage per mu is 20-30 kg.

This comparative example was the same as example 3 except that the sugar cane biomass char described in example 3 was not used.

Comparative example 2

A biological conditioner for regulating meta-acid soil is prepared from the following raw materials in parts by weight: 40 parts of sugarcane biochar, 30 parts of blast furnace slag powder, 20 parts of humic acid, 10 parts of zeolite, 5 parts of lime powder and 5 parts of microbial powder.

The preparation method of the sugarcane biochar comprises the following steps: drying and crushing sugarcane peel, pre-carbonizing the sugarcane peel in a muffle furnace for 3 hours at 200 ℃, introducing nitrogen, and heating at 5 ℃ for min in a nitrogen environment^-1Keeping the temperature at 500 ℃ for 1 h; at a temperature rise rate of 2 ℃ min^-1Keeping the temperature at 700 ℃ for 1h, and cooling to room temperature to obtain the sugarcane biochar.

The microbial powder is a mixture of bacillus subtilis powder and streptomyces albus powder, and the specific preparation method of the streptomyces albus powder comprises the following steps: inoculating Streptomyces albus preserved in a freeze-drying tube into a solid slant of a broth culture medium, and culturing at 30-32 ℃ for 48 hours; picking the bacterial colony to be inoculated into a sterilized liquid culture medium, and culturing for 36 hours under the conditions of 28 ℃, 150-; and (4) spray-drying the fermentation liquor to obtain the streptomyces albus powder.

The liquid culture medium comprises the following components in parts by weight: 20 parts of sucrose, 5 parts of sodium chloride, 20 parts of peptone, 5 parts of magnesium sulfate, 3 parts of monopotassium phosphate and 1000 parts of water.

The Bacillus subtilis can be commercially available bacterial powder, and Streptomyces albus strain (Streptomyces albus) is purchased from China general microbiological culture Collection center (address: China academy of sciences, China, No. 3, West Lu No. 1, Beijing, Chaoyang, and has a preservation number of CGMCC4.7658 and a preservation date of 2019, 8 months and 23 days.

The viable count of the bacillus subtilis and the streptomyces albus in the microbial powder is respectively more than 30 hundred million/g and more than 1 hundred million/g.

A method for preparing a biological conditioner for conditioning meta-acid soil, comprising the following steps:

(1) preparing the sugarcane biochar: drying and crushing sugarcane peel, pre-carbonizing the sugarcane peel in a muffle furnace for 3 hours at 200 ℃, introducing nitrogen, and heating at 5 ℃ for min in a nitrogen environment^-1Keeping the temperature at 500 ℃ for 1 h; at a temperature rise rate of 2 ℃ min^-1Keeping the temperature at 700 ℃ for 1h, and cooling to room temperature to obtain the sugarcane biochar;

(2) mixing the raw materials according to the weight part, granulating by a granulator, controlling the particle size to be 3-6mm, fully drying after the granulation is finished, packaging and warehousing. Binders such as modified starch can be added during the granulation process to facilitate granulation.

A biological conditioner for regulating acid soil is prepared by applying the biological conditioner to the surface of acidic soil in field, ditch or hole, or mixing with other fertilizers, and the dosage per mu is 20-30 kg.

In this comparative example, commercial blast furnace slag powder was used as it is, and the other raw materials and preparation method were the same as in example 3, except that the blast furnace slag powder was not treated.

Comparative example 3

A biological conditioner for regulating meta-acid soil is prepared from the following raw materials in parts by weight: 40 parts of sugarcane biochar, 30 parts of blast furnace slag powder, 20 parts of humic acid, 10 parts of zeolite, 5 parts of lime powder and 5 parts of microbial powder.

The preparation method of the sugarcane biochar comprises the following steps: drying and crushing sugarcane peel, pre-carbonizing the sugarcane peel in a muffle furnace for 3 hours at 200 ℃, introducing nitrogen, and heating at 5 ℃ for min in a nitrogen environment^-1Keeping the temperature at 500 ℃ for 1 h; at a temperature rise rate of 2 ℃ min^-1Keeping the temperature at 700 ℃ for 1h, and cooling to room temperature to obtain the sugarcane biochar.

The preparation method of the blast furnace slag powder comprises the following steps: ball-milling the blast furnace slag until the specific surface area is 300-²And/kg, adding a sodium hydroxide solution, soaking for 5 hours, drying, adding 3 wt% of an alkaline activator, mixing and ball-milling for 2 hours again to obtain blast furnace slag powder.

The alkali activator is silica fume and calcium oxide 1: 1 and mixing.

The concentration of the sodium hydroxide solution is 1 mol/L.

The microbial powder is bacillus subtilis powder.

The viable count of the bacillus subtilis in the microbial powder is more than 30 hundred million/g

A method for preparing a biological conditioner for conditioning meta-acid soil, comprising the following steps:

(1) preparing the sugarcane biochar: drying and crushing sugarcane peel, pre-carbonizing the sugarcane peel in a muffle furnace for 3 hours at 200 ℃, introducing nitrogen, and heating at 5 ℃ for min in a nitrogen environment^-1Keeping the temperature at 500 ℃ for 1 h; at a temperature rise rate of 2 ℃ min^-1Keeping the temperature at 700 ℃ for 1h, and cooling to room temperature to obtain the sugarcane biochar;

(2) preparing blast furnace slag powder: ball-milling the blast furnace slag until the specific surface area is 300-²Adding sodium hydroxide solution to soak for 5 hours, drying, adding 3 wt% of alkaline activator, mixing and ball-milling for 2 hours again to obtain blast furnace slag powder;

(3) mixing the raw materials according to the weight part, granulating by a granulator, controlling the particle size to be 3-6mm, fully drying after the granulation is finished, packaging and warehousing. Binders such as modified starch can be added during the granulation process to facilitate granulation.

A biological conditioner for regulating acid soil is prepared by applying the biological conditioner to the surface of acidic soil in field, ditch or hole, or mixing with other fertilizers, and the dosage per mu is 20-30 kg.

The comparative example is the same as example 3 except that the Streptomyces albus powder is not added.

Test verification

1. Test article

The apple variety is red Fuji, and the planting density is 700 plants/hm²The ages of the trees are the same and the tree vigor is consistent.

2. Test soil

The acidification is serious, the soil physical and chemical properties are that the type is brown soil, and the basic physical and chemical properties of the soil layer are as follows: pH 4.60, organic matter 7.05 g.kg^-1Effective nitrogen 92.4 mg/kg^-150.4 mg/kg of available phosphorus^-1Effective potassium 131.5mg·kg^-1Volume weight of 1.5g/cm³。

3. Design of experiments

The test was carried out in five treatment groups, respectively: s1 example 3 conditioner + conventional fertilization; s2 comparative example 1 conditioner

Conventional fertilization; s3 conditioner of comparative example 2 + conventional fertilization; s4 conditioner of comparative example 3 + conventional fertilization; s5 adding a commercially available acidified soil conditioner and conventional fertilization; the application rates of S1-S4 are all 30 kg/mu, and the commercially available soil conditioner is applied according to the requirements.

The test is continuously carried out for 2 years, the soil conditioner is applied for 1 time per year in a basal mode, the fertilization time is consistent with the first application time of the conventional fertilization, the conventional fertilization is divided into two times, the first fertilization time is 3 and 15 days in 2018 and 2 and 20 days in 2019, and the conventional fertilization is applied with an organic-inorganic compound fertilizer (organic matter is more than or equal to 20 percent, N-P is more than or equal to 20 percent, and the content of N-P is equal to or less than that of N-P₂O₅-K₂15-5-10)2200kg/hm²The second fertilization time is that the compound fertilizer (N-P) is applied for 22 days in 6 months in 2018 and 17 days in 6 months in 2019₂O₅-K₂O is 15-15-15)3500kg/hm². Each treatment was repeated 4 times, 3 trees were repeated one time, and 5 treatments consisted of 60 trees. And digging holes at the position 1m away from the trunk of the fruit tree below the crown in a fertilizing mode, wherein the depth of each hole is about 20cm, the diameter of each hole is about 25cm, and 8 holes are dug in each fruit tree. Except different experimental fertilizers, other agricultural measures and field management of each community are consistent.

4. Sampling method

In the apple maturity stage in 10 months in 2018 and 10 months in 2019, 12 sample fruits are randomly harvested from 4 directions of south, east and north according to treatment, and the fruit trees are taken back to a laboratory for subsequent single fruit weight and quality index determination. The soil sample is selected from a diagonal 5-point sampling method to collect a plough layer soil sample of 0-20cm, about 150g of the soil sample is reserved by a quartering method, the soil sample is taken back to a laboratory, dried in the air and ground, and then the ground soil sample is respectively sieved by a 10-mesh sieve and a 60-mesh sieve for subsequent index determination of the soil. The fruits are harvested at one time respectively, and the actual harvest amount is taken as the yield of each cell.

5. Test method

Measuring soluble solid of the fruit by using an automatic digital refractometer (ATAGO RX-5000 alpha); vitamin C

(Vc) is determined by 2, 6-dichlorophenol indophenol sodium titration; the total sugar content is reduced by acid hydrolysis copper-direct titration method;

titratable acids were determined by NaOH neutralization titration.

Measuring the volume weight of the soil by adopting a cutting ring method; measuring the porosity of the soil by adopting a drying method, taking a required soil sample by using a cutting ring, weighing, then putting the soil sample into a drying oven, drying for 24 hours at 105 ℃, taking out and weighing; the pH value of the soil is measured by adopting a water-soil ratio of 5:1 and an acidimeter; the soil exchangeable acid adopts potassium chloride exchange and neutralization titration, and the soil available phosphorus adopts sodium bicarbonate molybdenum antimony colorimetry; the effective potassium is obtained by an ammonium acetate flame photometer, organic matters are obtained by a potassium dichromate volumetric method, urease is obtained by a phenol-sodium hypochlorite colorimetric method, catalase is obtained by a titration method, and sucrase is obtained by a 3, 5-dinitrosalicylic acid colorimetric method.

The results of the experiment are shown in tables 1-2:

TABLE 1 apple quality test results

TABLE 2 soil conditions

The apples obtained by the soil conditioner test group in example 3 of the present invention had higher yield and quality than the comparative examples in which the composition of the raw materials was changed. Similarly, the soil test result shows that the soil conditioner can effectively improve the physical and chemical properties of soil, reduce the volume weight of the soil and improve the activity of soil enzyme, thereby promoting the absorption and utilization of nutrient substances by crops and improving the yield and quality of fruits. The raw materials are cooperatively matched, so that the effect is weak in the absence of one raw material, and the physical and chemical properties of the acidified soil are effectively adjusted.

It should be noted that the above-mentioned embodiments are only some of the preferred modes for implementing the invention, and not all of them. Obviously, all other embodiments obtained by persons of ordinary skill in the art based on the above-mentioned embodiments of the present invention without any creative effort shall fall within the protection scope of the present invention.