阅读说明：本技术 一种促进口腔软硬组织原位再生修复的口香糖及其制备方法 (Chewing gum for promoting in-situ regeneration and repair of soft and hard tissues of oral cavity and preparation method thereof ) 是由 吴昌鸿 于 2021-09-02 设计创作，主要内容包括：本发明提供一种促进口腔软硬组织原位再生修复的口香糖,包括以下重量份数的组分：再生修复材料：1～30份；胶基：20～50份；甜味剂：10～40份；甘油：0.7～2份；所述再生修复材料包括以下摩尔分数的成分：SiO-(2)：42.0～47.0％,CaO：28.0～32.0％,SrO：8.0～12.0％,Na-(2)O：4.0～7.0％,MgO：4.0～6.0％,P-(2)O-(5):1.5～3.5％。本发明通过将再生修复材料、胶基、赤藓糖醇、香精,甘油采用特定方式进行配比混合,并以口香糖的形态提供,可以同时达到口腔软硬组织原位再生修复的作用。本发明还提供了一种促进口腔软硬组织原位再生修复的口香糖的制备方法。(The invention provides a chewing gum for promoting in-situ regeneration and repair of soft and hard tissues of an oral cavity, which comprises the following components in parts by weight: regeneration and repair materials: 1-30 parts; gum base: 20-50 parts of a solvent; a sweetening agent: 10-40 parts; glycerol: 0.7-2 parts; the regenerative repair material comprises the following components in mole fraction: SiO2 2 ：42.0～47.0％，CaO：28.0～32.0％，SrO：8.0～12.0％，Na 2 O：4.0～7.0％，MgO：4.0～6.0％，P 2 O 5 1.5 to 3.5 percent. The regeneration and repair material, the gum base, the erythritol, the essence and the glycerol are mixed in a specific mode and provided in the form of chewing gum, so that the in-situ regeneration and repair effect of soft and hard tissues of the oral cavity can be achieved at the same time. The invention also provides a method for promoting the regeneration of soft and hard tissues in the oral cavity in situA method for preparing a repaired chewing gum.)

1. A chewing gum for promoting in-situ regeneration and repair of soft and hard tissues of an oral cavity comprises the following components in parts by weight:

regeneration and repair materials: 1-30 parts;

gum base: 20-50 parts of a solvent;

a sweetening agent: 10-40 parts;

glycerol: 0.7-2 parts;

the regenerative repair material comprises the following components in mole fraction:

SiO₂：42.0～47.0％，CaO：28.0～32.0％，SrO：8.0～12.0％，Na₂O：4.0～7.0％，MgO：4.0～6.0％，P₂O₅:1.5～3.5％。

2. a chewing gum according to claim 1, wherein the particle size of the regenerative repair material is 0.03 to 50 μm.

3. The chewing gum according to claim 1, wherein the pH value of the chewing gum for promoting in-situ regeneration and repair of soft and hard tissues in the oral cavity is 7.0-8.5.

4. A chewing gum according to claim 1, wherein the sweetener is erythritol.

5. The chewing gum according to claim 1, wherein the chewing gum for promoting in-situ regeneration and repair of soft and hard tissues of the oral cavity further comprises 0.3-2 parts of essence.

6. The method for preparing chewing gum for promoting in-situ regeneration and repair of soft and hard tissues in oral cavity according to claim 1, comprising the following steps:

A) mixing the gum base softened by heating, the regeneration repair material, the sweetening agent and the glycerol under the stirring condition to obtain a mixed material;

B) extruding and molding the mixed material to obtain a sugar blank;

C) and (3) aging the sugar blank to obtain the chewing gum for promoting in-situ regeneration and repair of soft and hard tissues of the oral cavity.

7. The preparation method according to claim 6, wherein the heating temperature of the gum base is 50-60 ℃; the heating time is 5-10 min.

8. The preparation method according to claim 6, wherein the stirring temperature in the step A) is 40-50 ℃; the stirring time is 10-15 min.

9. The method according to claim 6, wherein the temperature of the extrusion in the step B) is 40 to 50 ℃.

10. The method according to claim 6, wherein the aging time in the step C) is 12 to 24 hours.

Technical Field

The invention belongs to the technical field of biomedical materials, and particularly relates to chewing gum for promoting in-situ regeneration and repair of soft and hard tissues of an oral cavity and a preparation method thereof.

Background

Caries and periodontal disease are the most common, frequent oral diseases. The result of the 'fourth national oral health epidemiological survey' issued in 2017 shows that the oral health condition of residents in China is happy and anxious, and 60-90% of children are seriously affected by decayed teeth, but most of the children cannot be effectively treated due to the conditions of oral health, economic reasons, lack of oral professional technology and the like. The causes of dental caries are many, such as unhealthy diet, bad oral hygiene, excessive intake of sugar, and various carbonated drinks. Can directly damage enamel, soften and loosen mineral substances on the surface of teeth, seriously cause the mineral to fall off, expose dentin and further cause dental caries. Therefore, protecting the tooth enamel is the primary barrier to caries. Periodontal disease mainly includes gingivitis and periodontitis, where periodontitis is usually the result of a broken gum and further worsening of gingivitis. Once the periodontal disease is acquired, untimely treatment may cause tooth loss. The report shows that the gum bleeding detection rate of residents aged 35 to 44 years in China is up to 87.4 percent.

How to improve the environmental sanitation of the oral cavity and reduce the caries and the periodontal disease is a problem to be solved urgently at present. There are few products specially used for tooth restoration in the market, and the toothpaste is mainly of a restoration type. The repairing toothpaste sold in the market is mainly the toothpaste containing hydroxyapatite or calcium sodium phosphosilicate, and in addition, bioactive glass such as conventional 45S5 is introduced into the toothpaste to obtain a certain repairing effect. The products have the following disadvantages:

1) the repair speed is slow, and the long-term durable use is required. Also, when used as a toothpaste, the residence time in the mouth is very short, resulting in very limited effectiveness.

2) When the bioactive glass particles are contacted with liquid, the surface of the bioactive glass particles rapidly reacts to cause the local pH value to be rapidly increased, the pH value can reach 13 at most, strong alkalinity is formed, certain cytotoxicity is realized, and certain pain is brought to patients in the using process.

The mouthwash mainly aims at periodontal diseases, is conventional mouthwash, has single function, mainly plays a role in resisting and inhibiting bacteria, and does not have a regeneration and repair function.

People suffering from oral diseases are likely to suffer from dental caries and periodontal diseases at the same time, so that the product with single function cannot meet the market demand.

Disclosure of Invention

The chewing gum has higher biological activity, can quickly take effect, can simultaneously realize in-situ regeneration and repair of tooth injury and periodontal injury, and has no discomfort in the using process.

The invention provides a chewing gum for promoting in-situ regeneration and repair of soft and hard tissues of an oral cavity, which comprises the following components in parts by weight:

regeneration and repair materials: 1-30 parts;

gum base: 20-50 parts of a solvent;

a sweetening agent: 10-40 parts;

glycerol: 0.7-2 parts;

the regenerative repair material comprises the following components in mole fraction:

SiO₂：42.0～47.0％，CaO：28.0～32.0％，SrO：8.0～12.0％，Na₂O：4.0～7.0％，MgO：4.0～6.0％，P₂O₅:1.5～3.5％。

preferably, the particle size of the regenerative repair material is 0.03-50 μm.

Preferably, the pH value of the chewing gum for promoting the in-situ regeneration and repair of soft and hard tissues of the oral cavity is 7.0-8.5.

Preferably, the sweetener is erythritol.

Preferably, the chewing gum for promoting in-situ regeneration and repair of soft and hard tissues of the oral cavity further comprises 0.3-2 parts of essence.

The invention provides a preparation method of chewing gum for promoting in-situ regeneration and repair of soft and hard tissues of an oral cavity, which comprises the following steps:

A) mixing the gum base softened by heating, the regeneration repair material, the sweetening agent and the glycerol under the stirring condition to obtain a mixed material;

B) extruding and molding the mixed material to obtain a sugar blank;

C) and (3) aging the sugar blank to obtain the chewing gum for promoting in-situ regeneration and repair of soft and hard tissues of the oral cavity.

Preferably, the heating temperature of the gum base is 50-60 ℃; the heating time is 5-10 min.

Preferably, the stirring temperature in the step A) is 40-50 ℃; the stirring time is 10-15 min.

Preferably, the extrusion temperature in the step B) is 40-50 ℃.

Preferably, the aging time in the step C) is 12-24 hours.

The invention provides a chewing gum for promoting in-situ regeneration and repair of soft and hard tissues of an oral cavity, which comprises the following components in parts by weight: regeneration and repair materials: 1-30 parts; gum base: 20-50 parts of a solvent; a sweetening agent: 10-40 parts; glycerol: 0.7-2 parts; the regenerative repair material comprises the following components in mole fraction: SiO2₂：42.0～47.0％，CaO：28.0～32.0％，SrO：8.0～12.0％，Na₂O：4.0～7.0％，MgO：4.0～6.0％，P₂O₅1.5 to 3.5 percent. The regeneration and repair material, the gum base, the erythritol, the essence and the glycerol are mixed in a specific mode and provided in the form of chewing gum, so that the in-situ regeneration and repair effect of soft and hard tissues of the oral cavity can be achieved at the same time. For soft tissues with oral injuries, such as gum damage, oral ulcer, gingivitis, periodontitis and the like, the product promotes the healing acceleration of wounds by guiding the ordered creeping substitution of tissues such as cells, extracellular matrixes, blood vessels and the like, realizes the ordered growth of fiber layers of new tissues, and further realizes the regeneration and repair of the injured tissues. For the hard tissues of oral cavity injury, such as decayed teeth, tooth demineralization, microcracks and the like, the regeneration and repair material contained in the product has higher reactivity and bioactivity, reacts quickly when contacting with saliva, has faster therapeutic ion release, forms hydroxyapatite in situ on the surface of the injured teeth, realizes the regeneration and repair of the hard tissues of the oral cavity, is applied in the form of chewing gum, promotes the secretion of the saliva on one hand during chewing, and has longer retention time in the oral cavity on the other hand, so that the product has more sufficient reaction and higher repair effect. Meanwhile, the pH value of the mixture is 7.0-8.5, which is relatively close to the pH value of a human body, and the mixture has small irritation to the human body and no cytotoxicity in the using process.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the provided drawings without creative efforts.

FIG. 1 is an SEM image of a tooth with an exposed dentinal ostiole of the present invention;

FIG. 2 is an SEM photograph of the tooth surface after gum treatment in example 2 of the present invention;

FIG. 3 is an EDS elemental analysis of the tooth surface after gum treatment in example 2 of the present invention;

FIG. 4 is an SEM image of the tooth surface after treatment with a control of the present invention.

Detailed Description

The invention provides chewing gum for promoting in-situ regeneration and repair of soft and hard tissues of an oral cavity, which comprises the following components in parts by weight:

regeneration and repair materials: 1-30 parts;

gum base: 20-50 parts of a solvent;

a sweetening agent: 10-40 parts;

glycerol: 0.7-2 parts;

the regenerative repair material comprises the following components in mole fraction:

SiO₂：42.0～47.0％，CaO：28.0～32.0％，SrO：8.0～12.0％，Na₂O：4.0～7.0％，MgO：4.0～6.0％，P₂O₅:1.5～3.5％。

in the present invention, in the regenerative repair material, SiO₂The molar fraction (b) is preferably 42.0 to 47.0%, more preferably 43.0 to 46.0%, such as 42.0%, 43.0%, 44.0%, 45.0%, 46.0%, 47.0%, and preferably a range value having any of the above values as an upper limit or a lower limit.

The molar fraction of CaO is preferably 28.0 to 32.0%, more preferably 29.0 to 31.0%, such as 28.0%, 29.0%, 30.0%, 31.0%, 32.0%, and preferably ranges in which any of the above values is an upper limit or a lower limit.

The molar fraction of SrO is preferably 8.0 to 12.0%, more preferably 9.0 to 11.0%, such as 8.0%, 9.0%, 10.0%, 11.0%, 12.0%, and preferably ranges from any of the above values as upper or lower limits.

Na₂The mole fraction of O is preferably 4.0 to 7.0%, more preferably 5.0 to 6.0%, such as 4.0%, 5.0%, 6.0%, 7.0%, and preferably ranges in which any of the above values is an upper limit or a lower limit.

The molar fraction of MgO is preferably 4.0 to 6.0%, more preferably 5.0 to 6.0%, such as 4.0%, 5.0%, 6.0%, and preferably ranges from any of the above values as upper or lower limits.

P₂O₅The molar fraction (c) is preferably 1.5 to 3.5%, more preferably 2.0 to 3.0%, such as 1.5%, 2.0%, 3.0%, 3.5%, and preferably ranges from any of the above values as upper or lower limits.

Ca and P in the regenerative repair material are main components of bones and teeth, Ca ions and P ions are uniformly distributed in a three-dimensional network structure rearranged by silicon bonds, the degradation speed is high, the wound healing is accelerated by guiding the ordered creeping substitution of tissues such as cells, extracellular matrix, blood vessels and the like, and the ordered growth of a new tissue fiber layer is realized; when the oral hard tissue regeneration liquid contacts with saliva, the oral hard tissue regeneration liquid reacts quickly, therapeutic ions are released quickly, hydroxyapatite is formed on the surface of the damaged tooth in situ, and regeneration and repair of the oral hard tissue are achieved.

In the present invention, the weight part of the regenerative repair material is preferably 1 to 30 parts, more preferably 10 to 25 parts, such as 1 part, 5 parts, 10 parts, 15 parts, 20 parts, 25 parts or 30 parts, and preferably any of the above values is a range with an upper limit or a lower limit.

In the invention, the particle size of the regenerative repair material is preferably 0.03-50 μm, and more preferably 0.05-0.2 μm.

In the invention, the gum base has a shaping effect and does not react with the surface of the regenerative repair material, so that the product has good stability during storage and does not lose the treatment effect. The gum base is preferably 20-50 parts by weight, more preferably 25-45 parts by weight, such as 20 parts, 25 parts, 30 parts, 35 parts, 40 parts, 45 parts and 50 parts by weight, and preferably ranges with any value as an upper limit or a lower limit.

In the invention, the sweetener is preferably erythritol, and the erythritol can play a role of the sweetener, can play a role in synergistic repair of hard tissues and can promote in-situ repair of tooth remineralization. The weight portion of the sweetener is preferably 10 to 40 parts, more preferably 20 to 30 parts, such as 10 parts, 15 parts, 20 parts, 25 parts, 30 parts, 35 parts and 40 parts, and preferably any value is a range with an upper limit or a lower limit.

In the invention, the glycerin has the synergistic repair effect on soft tissues, has the effects of promoting the absorption of a skin nervous system, healing wound tissues and the like, and can enable the regenerative repair material to better exert the wound healing and repairing regeneration effects. The weight portion of the glycerin is preferably 0.7 to 2 parts, more preferably 1 to 1.5 parts, such as 0.7 part, 1.0 part, 1.5 parts, 2.0 parts, and preferably a range value with any of the above values as an upper limit or a lower limit.

In the invention, the formula of the chewing gum also comprises essence, the specific type of the essence is not particularly limited, and the essence can be edible essence commonly used in the field according to the taste requirement. The weight portion of the essence is preferably 0.3-2 parts, more preferably 0.5-1.5 parts, such as 0.3 part, 0.5 part, 1.0 part, 1.5 part and 2.0 parts, and preferably the range value taking any value as the upper limit or the lower limit.

The pH value of the chewing gum is 7.0-8.5, is relatively close to the pH value of a human body, and has small irritation to the human body and no cytotoxicity in the using process.

The invention also provides a preparation method of the chewing gum for promoting in-situ regeneration and repair of soft and hard tissues of the oral cavity, which comprises the following steps:

A) mixing the gum base softened by heating, the regeneration repair material, the sweetening agent and the glycerol under the stirring condition to obtain a mixed material;

B) extruding and molding the mixed material to obtain a sugar blank;

C) and (3) aging the sugar blank to obtain the chewing gum for promoting in-situ regeneration and repair of soft and hard tissues of the oral cavity.

The method comprises the steps of firstly heating and softening a gum base, then mixing the softened gum base with essence, glycerol, a sweetening agent and a regeneration repair material under the stirring condition, and uniformly stirring to obtain a mixed material.

In the present invention, the kinds and amounts of the gum base, essence, glycerin, sweetener and regenerative repair material are the same as those of the gum base, essence, glycerin, sweetener and regenerative repair material, and the present invention is not described herein again.

In the invention, the heating temperature of the gum base is preferably 50-60 ℃, and more preferably 55 ℃; the heating time is preferably 5-10 min.

In the invention, the stirring temperature is preferably 40-50 ℃, such as 45 ℃; the stirring time is preferably 10-15 min.

After the mixed material is obtained, the mixed material is extruded and molded to obtain the sugar blank, wherein the extrusion molding sequentially comprises two steps of extrusion and pressing, and the extrusion temperature is preferably 40-50 ℃, and more preferably 45 ℃.

The invention can select the extruded size and the pressed size according to the actual requirement, and if necessary, the extruded chewing gum sheet can be cut into pieces.

According to the invention, the extruded chewing gum tablet is aged, and the aging temperature is preferably selected; the aging time is preferably 12-24 hours, and the gum tablet is aged to reach the moisture balance to obtain the gum.

The invention provides a chewing gum for promoting in-situ regeneration and repair of soft and hard tissues of an oral cavity, which comprises the following components in parts by weight: regeneration and repair materials: 1-30 parts; gum base: 20-50 parts of a solvent; a sweetening agent: 10-40 parts; glycerol: 0.7-2 parts; the regenerative repair material comprises the following components in mole fraction: SiO2₂：42.0～47.0％，CaO：28.0～32.0％，SrO：8.0～12.0％，Na₂O：4.0～7.0％，MgO：4.0～6.0％，P₂O₅1.5 to 3.5 percent. The regeneration and repair material, the gum base, the erythritol, the essence and the glycerol are mixed in a specific mode and provided in the form of chewing gum, so that the in-situ regeneration and repair effect of soft and hard tissues of the oral cavity can be achieved at the same time. For soft tissues of oral lesions, e.g.The product promotes the healing acceleration of wounds by the action of guiding the tissues such as cells, extracellular matrixes, blood vessels and the like to crawl and replace in order, realizes the ordered growth of fiber layers of new tissues and further realizes the regeneration and repair of damaged tissues. For the hard tissues of oral cavity injury, such as decayed teeth, tooth demineralization, microcracks and the like, the regeneration and repair material contained in the product has higher reactivity and bioactivity, reacts quickly when contacting with saliva, has faster therapeutic ion release, forms hydroxyapatite in situ on the surface of the injured teeth, realizes the regeneration and repair of the hard tissues of the oral cavity, is applied in the form of chewing gum, promotes the secretion of the saliva on one hand during chewing, and has longer retention time in the oral cavity on the other hand, so that the product has more sufficient reaction and higher repair effect. Meanwhile, the pH value of the mixture is 7.0-8.5, which is relatively close to the pH value of a human body, and the mixture has small irritation to the human body and no cytotoxicity in the using process.

In order to further illustrate the present invention, the chewing gum and the preparation method thereof for promoting the regeneration and repair of soft and hard tissues in the oral cavity in situ provided by the present invention are described in detail with reference to the following examples, which should not be construed as limiting the scope of the present invention.

Examples 1 to 3

Chewing gum was prepared according to the raw material amounts in table 1 by the following method:

step S1: heating the gum base in an oven at 55 ℃ for 10min for softening, adding into a stirrer, sequentially adding essence, glycerol, erythritol solution and regeneration repair material in batches, keeping the stirring temperature at 45 ℃, and stirring for 15 min;

step S2: the uniformly stirred materials enter an extrusion forming stage, an extruder is kept at a constant temperature of about 45 ℃ to extrude a sugar blank with the thickness of 10mm and the width (400 +/-10), and the sugar blank has complete tissue, smooth surface and no sand hole cavity; and (3) entering a pressing link, pressing the sugar blank to the thickness of (4 +/-0.5) mm, and keeping the size of the cut block to be (0.75 +/-0.1) g.

Step S3: transferring the chewing gum sheet to an aging room, aging for more than 12h to make the chewing gum sheet reach moisture balance and age, and obtaining the chewing gum.

TABLE 1 raw material ratios (parts by weight) of examples 1 to 3

	Example 1	Example 2	Example 3
				Regenerative repair material	15	20	25
Gum bases	48	43	38
				Erythritol and its preparation method	35	35	35
Essence	0.5	0.5	0.5
				Glycerol	1.5	1.5	1.5

The compositions of the regenerative repair materials used in examples 1 to 3 were as follows:

SiO₂：43.0％，CaO：34.0％，SrO：10.0％，Na₂O：5.0％，MgO：5.0％，P₂O₅the rest is.

Sensory evaluation

In sensory evaluation, 10 food professionals were asked to taste and rate them according to sensory evaluation criteria (see table 2), and the results of the scoring were counted (table 3).

TABLE 2 sensory evaluation criteria

Item	Index (I)	Score of
			Taste of the product	Has comfortable taste, sweet taste and certain elasticity	25
Smell(s)	Pleasant fragrance	25
			Form of the composition	Smooth surface and moderate hardness	25
Color	Has luster	25

Table 3: comprehensive scoring results

	Example 1	Example 2	Example 3
				Score of	85	94	86

Experiments prove the pH, the soft tissue repair effect and the hard tissue repair effect of the product prepared by the invention.

1. Product pH value test and cytotoxicity test

The experimental method comprises the following steps: the product of the present invention was placed in 10ml of simulated oral Saliva (SOF) (specifically, 137.35mM NaCl, 0.90mM CaCl2, 3.01mM KCl, 7.17mM K2HPO 4.3H2O, 4.17mM NaHCO3, 1.53mM MgCl 2.6H2O, 20.00mM HCl, 0.51mM Na2SO4 and Tris adjusted to pH 7.0), chewed in a simulated chewing gum manner, repeatedly pounded in a mortar, ground for 10 minutes, and filtered, and the resulting filtrate was measured for pH using a precision pH meter.

Cytotoxicity was evaluated by using human epidermal fibroblasts and MTT method.

The test results are shown in table 4:

table 4: results of pH and cytotoxicity test

	Example 1	Example 2	Example 3
				pH value	7.51	7.69	7.82
Cytotoxicity	0	0	0

The detection result shows that the product of the invention has weak alkalinity in all contact with simulated oral saliva, is relatively close to the pH value of a human body, and has little pH change along with the increase of the regeneration and repair material amount in the product. For cytotoxicity, the results indicated that the cytotoxicity was 0, i.e., no cytotoxicity. Therefore, the product has no irritation or very little irritation to human body and high safety. The pH value of the formula of the special bioactive glass is as high as more than 10. Certain pain can be brought to the patient in the actual use process.

2. Soft tissue repair effect test

The healthy New Zealand white rabbits are 9, 4 months old, male and female, are randomly divided into 3 groups according to the body weight, and each group comprises 3 rabbits, and the groups respectively correspond to an invention group, a blank control group and a positive control group. The experiment was started: (1) establishing a dental ulcer model: narcotizing the ear vein of a rabbit with 3% sodium pentobarbital to pull open the upper lip and expose the mucous membrane of the lip, soaking glacial acetic acid with volume fraction of 40% by using a filter paper sheet with the prefabricated diameter of 5mm and the thickness of about 1mm, placing the filter paper sheet on two sides of the upper lip of the rabbit, and taking out the filter paper sheet for 60s to show that the area has 5.5mm white damage; ulcers with a diameter of about 5.5mm appeared in 48 hours; (2) the inventive group used fresh filtrate from example 2 described in experiment 1; the blank control group was not treated; the positive control group was applied with a proper amount of a similar marketed toothpaste containing a representative bioactive glass (ingredients 46.1 mol.% SiO2, 24.4 mol.% Na2O, 26.9 mol.% CaO, and 2.6 mol.% P2O5) and spread evenly; the medicine is added once a day, and the healing condition of the wound surface is observed. The results obtained are shown in Table 5:

TABLE 5 test results of soft tissue repair effect

	Time of healing	Healing effect
			Invention group	2d	Smooth, elastic, dark and non-infectious
Blank control group	9d	Unevenness, raised, dark, and red and swollen
			Positive control group	6d	Relatively flat, slightly convex and no red swelling

The experimental comparison results show that the invention group has obvious effect of promoting the soft tissue repair, can obviously shorten the healing period, and the healed wound surface is flat and smooth, and the repair effect is greatly improved.

3. Hard tissue repair effect test

Taking the dog teeth in vitro, after the dog teeth are embedded with a hot embedding machine, polishing and grinding the dog teeth respectively by using 80, 200, 400, 1000 and 2000 silicon carbide water sand paper until the surface is bright and flat, finally, polishing and smoothing the dog teeth by using polishing paper, and ultrasonically cleaning the dog teeth in deionized water for 3min for later use to obtain a sample with exposed dentin pinholes (a typical picture is shown in figure 1). Appropriate samples of dental enamel were selected and randomly divided into 2 groups of 5 each. The two groups were named as the invention group and the control group, respectively.

The treatment method comprises the following steps:

the inventive group was directly soaked in fresh filtrate obtained from example 2 described in test 1 (product pH test and cytotoxicity test) for 2 min. The control group was brushed for 2min with a commercial toothpaste containing representative bioactive glass (ingredients 46.1 mol.% SiO2, 24.4 mol.% Na2O, 26.9 mol.% CaO, and 2.6 mol.% P2O5) using a toothbrushing regimen.

Subsequently, each cured sample was taken out and dried at 60 ℃; after complete drying, the sample surface was sprayed with gold for SEM observation to characterize the microscopic state of the material surface, as shown in FIGS. 2-4. FIG. 2 is a Scanning Electron Microscope (SEM) topography of the surface of the material after the inventive group treatment. As can be seen from fig. 2, a layer of hydroxyapatite particles completely covered the surface of the sample, completely repairing the exposed dentinal pinholes; fig. 3 is an EDS elemental analysis chart of the surface of the corresponding material, and the results show Ca, P, O elements, confirming that hydroxyapatite is formed on the surface.

The test shows that the premixed strontium-doped calcium silicate-based bioactive preparation obtained by the invention group has very high bioactivity; while the control group formed partial hydroxyapatite under the same test conditions, most of the dentinal pinholes remained exposed and were not repaired, as shown in fig. 4.

Therefore, the chewing gum for promoting the in-situ regeneration and repair of soft and hard tissues in the oral cavity has higher biological activity and quick response.

The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.