阅读说明：本技术 具有诱导肿瘤细胞凋亡的组合物及制备方法及用途 (Composition capable of inducing tumor cell apoptosis and preparation method and application thereof ) 是由 谭娟 于 2021-09-07 设计创作，主要内容包括：本发明公开了一种具有诱导肿瘤细胞凋亡的组合物及制备方法及用途,所述组合物按质量百分比由下述组分制成：山药小分子肽10％-20％；香菇糖肽5％-10％；藻油粉10％和膨化米粉60％-70％。本发明通过研究香菇糖肽和山药小分子肽的配比,提高了组合物促进肿瘤细胞凋亡作用,促进了人体吸收,改善营养效果显著。本发明的方法优化了原料的添加顺序,疏松堆密度,使混合物一、混合物二、混合物三疏松堆密度接近,制备得到的组合物质量稳定,产品外观颜色一致,口感细腻,提高产品营养均匀性。(The invention discloses a composition capable of inducing tumor cell apoptosis, a preparation method and application thereof, wherein the composition is prepared from the following components in percentage by mass: 10% -20% of yam small molecular peptide; 5% -10% of lentinan; 10% of algae oil powder and 60% -70% of puffed rice flour. According to the invention, by researching the proportion of the lentinan peptide and the Chinese yam micromolecule peptide, the effect of the composition on promoting tumor cell apoptosis is improved, the absorption of a human body is promoted, and the nutrition improving effect is obvious. The method optimizes the adding sequence and the loose bulk density of the raw materials, so that the loose bulk densities of the mixture I, the mixture II and the mixture III are close to each other, the prepared composition has stable quality, the appearance and the color of the product are consistent, the taste is fine and smooth, and the nutrition uniformity of the product is improved.)

1. The composition capable of inducing tumor cell apoptosis is characterized by being prepared from the following components in percentage by mass:

10% -20% of yam small molecular peptide; 5% -10% of lentinan; 10% of algae oil powder and 60% -70% of puffed rice flour.

2. The composition according to claim 1, characterized by consisting of, in mass percent:

20% of yam small molecular peptide; 10% of lentinan; 10% of algae oil powder; 60 percent of puffed rice flour.

3. The composition as claimed in claim 1 or 2, wherein the yam small molecule peptide has a peptide mass content of 80% or more, and a peptide mass content of 75% or more with a relative molecular weight of less than 1000 Da.

4. The composition as claimed in claim 1 or 2, wherein the glycopeptide of Lentinus edodes has a peptide mass content of 80% or more and a peptide mass content of 60% or more with a relative molecular weight of 500 Da or less.

5. The composition according to claim 1 or 2, wherein the algal oil powder has a DHA content of 10% by mass or more.

6. Method for the preparation of a composition with induction of apoptosis of tumor cells according to any one of claims 1 to 5, characterized in that it comprises the following steps:

(1) weighing Chinese yam micromolecule peptide according to the mass percentage; lentinan; algae oil powder and puffed rice flour;

(2) dividing the puffed rice flour into a part A, a part B and a part C according to the mass, wherein the mass of the part A is the same as that of the yam micromolecule peptide; the mass of the part B is equal to that of the lentinan peptide, and the mass of the part C is the sum of the mass of the puffed rice flour minus the mass of the part A and the mass of the part B;

(3) airflow mixing the yam small molecular peptide and the part A of puffed rice flour for 15-25min to obtain a mixture I; airflow mixing Lentinus Edodes glycopeptide and part B of puffed rice flour for 15-25min to obtain mixture II; and (3) mixing the algae oil powder and the C part puffed rice flour for 5-15min in an air flow manner to obtain a mixture III, adding the mixture I and the mixture II into the mixture III, continuing to mix for 20-30min in an air flow manner, and drying to obtain the composition for inducing tumor cell apoptosis.

7. The method of claim 6, wherein the gas streams are mixed by: starting the stirring paddle, wherein the speed of the stirring paddle is 6r/s, and simultaneously blowing air with the temperature of 20-30 ℃, the relative humidity of 80-90% and the air speed of 7m/s to boil the materials.

8. The method according to claim 6, wherein the drying is: air with the temperature of 60 ℃ and the relative humidity of 20-30% is introduced for 5-10 minutes at the wind speed of 10 m/s.

9. Use of a composition according to any one of claims 1 to 5 for the preparation of an anticancer food product.

Technical Field

The invention belongs to the field of medicines and foods, and particularly relates to a composition capable of inducing tumor cell apoptosis, a preparation method and application thereof.

Background

At present, about 40% -80% of tumor patients with malnutrition are killed directly by the malnutrition in about 20% of tumor patients.

The cause of malnutrition in patients with tumors is that tumors are a wasting disease, leading to a reduction in proteins in the body. The formula food of the composition for inducing the tumor cell apoptosis is developed, the metabolic absorption abnormality of the tumor patients is regulated, the nutritional requirements of the tumor patients are met, the incidence rate of malnutrition of the tumor patients is reduced, and the positive effect is achieved. The protein source is mainly divided into whole protein, protein hydrolysate-peptide and the like. The whole protein is decomposed into amino acids or protein hydrolysate, namely peptide, in the process of human digestion, and the protein is also partially decomposed into human harmful substances such as ammonia, keto acid, urea and the like, so that side effects are generated on human bodies. The absorption and utilization rate of the whole protein is 70 to 80 percent usually by human bodies. When a human body takes in whole protein, energy is consumed to digest and decompose the whole protein, so that the burden of a liver is increased, dyspepsia is easily caused, and the function of a kidney is influenced.

Disclosure of Invention

The invention aims to overcome the defects of the prior art and provide a composition for inducing tumor cell apoptosis.

The second purpose of the invention is to provide a preparation method of the composition for inducing tumor cell apoptosis.

The third purpose of the invention is to provide the application of the composition for inducing tumor cell apoptosis in preparing anti-cancer food.

The technical scheme of the invention is summarized as follows:

a composition for inducing tumor cell apoptosis is prepared from the following components in percentage by mass: 10% -20% of yam small molecular peptide; 5% -10% of lentinan; 10% of algae oil powder and 60% -70% of puffed rice flour.

Preferably, the composition consists of the following components in percentage by mass: 20% of yam small molecular peptide; 10% of lentinan; 10% of algae oil powder; 60 percent of puffed rice flour.

The yam small molecular peptide has a peptide mass content of more than or equal to 80 percent, and a peptide mass content of less than 1000Da relative to the molecular weight of more than or equal to 75 percent.

The lentinan peptide has a peptide mass content of more than or equal to 80 percent and a peptide mass content of more than or equal to 60 percent with a relative molecular weight of less than or equal to 500-1000 Da.

The DHA content in the algae oil powder is more than or equal to 10 percent.

The preparation method of the following composition for inducing tumor cell apoptosis comprises the following steps:

(1) weighing Chinese yam micromolecule peptide according to the mass percentage; lentinan; algae oil powder and puffed rice flour;

(2) dividing the puffed rice flour into a part A, a part B and a part C according to the mass, wherein the mass of the part A is the same as that of the yam micromolecule peptide; the mass of the part B is equal to that of the lentinan peptide, and the mass of the part C is the sum of the mass of the puffed rice flour minus the mass of the part A and the mass of the part B;

(3) airflow mixing the yam small molecular peptide and the part A of puffed rice flour for 15-25min to obtain a mixture I; airflow mixing Lentinus Edodes glycopeptide and part B of puffed rice flour for 15-25min to obtain mixture II; and (3) mixing the algae oil powder and the C part puffed rice flour for 5-15min in an air flow manner to obtain a mixture III, adding the mixture I and the mixture II into the mixture III, continuing to mix for 20-30min in an air flow manner, and drying to obtain the composition for inducing tumor cell apoptosis.

The gas flow mixing is as follows: starting the stirring paddle, wherein the speed of the stirring paddle is 6r/s, and simultaneously blowing air with the temperature of 20-30 ℃, the relative humidity of 80-90% and the air speed of 7m/s to boil the materials.

The drying comprises the following steps: air with the temperature of 60 ℃ and the relative humidity of 20-30% is introduced for 5-10 minutes at the wind speed of 10 m/s.

The application of the composition in preparing anticancer food is provided.

The invention has the advantages that:

the composition and the dosage ratio of the components are optimized, and particularly, the ratio of the lentinan peptide to the yam micromolecular peptide is researched, so that the effect of the composition on promoting tumor cell apoptosis is improved, the absorption of a human body is promoted, and the nutrition improving effect is obvious.

The method optimizes the adding sequence and the loose bulk density of the raw materials, so that the loose bulk densities of the mixture I, the mixture II and the mixture III in the step (4) are close to each other, the prepared composition has stable quality, the appearance and the color of the product are consistent, the taste is fine and smooth, and the nutrition uniformity of the product is improved.

The composition prepared by the invention has obvious effect of improving immunity for people of 10 years old and older, has no side effect, and avoids adverse consequences such as protein allergy and the like.

Detailed Description

The yam small molecule peptide is purchased from Hibiscus parviensis Biotech, Inc.

The lentinan is purchased from Hibiscus Biotech, Inc.

Algal oil powder was purchased from Qingdaoyuangkang biotechnology limited.

The puffed rice flour meets the national standard of GB 17401-2014 food safety puffed food.

The sources of the components are disclosed to enable one skilled in the art to practice the invention, but are not intended to limit the sources of the components of the invention, and other companies' corresponding products meeting the requirements may be used in the invention.

The present invention will be further illustrated by the following specific examples.

Example 1

A composition for inducing tumor cell apoptosis is prepared from the following components in percentage by mass: 20kg of Chinese yam small molecular peptide; 10kg of lentinan peptide; 10kg of algae oil powder; 60kg of puffed rice noodles.

The mass content of the yam small molecular peptides is more than or equal to 80 percent, and the mass content of the peptides with the relative molecular weight less than 1000Da is more than or equal to 75 percent.

The lentinan has a peptide mass content of 80% or more, and a peptide mass content of 60% or more with a relative molecular weight of 500-1000Da or less.

The DHA content in the algae oil powder is more than or equal to 10 percent.

Example 2

A composition for inducing tumor cell apoptosis is prepared from the following components in percentage by mass: 10kg of Chinese yam micromolecule peptide; 10kg of lentinan peptide; 10kg of algae oil powder; puffed rice flour 70 kg.

The mass content of the yam small molecular peptides is more than or equal to 80 percent, and the mass content of the peptides with the relative molecular weight less than 1000Da is more than or equal to 75 percent.

The lentinan has a peptide mass content of 80% or more, and a peptide mass content of 60% or more with a relative molecular weight of 500-1000Da or less.

The DHA content in the algae oil powder is more than or equal to 10 percent.

Example 3

A composition for inducing tumor cell apoptosis is prepared from the following components in percentage by mass: 15kg of Chinese yam micromolecule peptide; 5kg of lentinan peptide; 10kg of algae oil powder; puffed rice flour 70 kg.

The mass content of the yam small molecular peptides is more than or equal to 80 percent, and the mass content of the peptides with the relative molecular weight less than 1000Da is more than or equal to 75 percent.

The lentinan has a peptide mass content of 80% or more, and a peptide mass content of 60% or more with a relative molecular weight of 500-1000Da or less.

The DHA content in the algae oil powder is more than or equal to 10 percent.

Example 4

The preparation method of the composition for inducing the tumor cell apoptosis comprises the following steps:

(1) weighing 20Kg of yam micromolecule peptide according to the mass; 10Kg of lentinan; 10Kg of algae oil powder; 60Kg of puffed rice flour (composition of example 1);

(2) dividing the puffed rice flour into three parts A, B and C according to the mass, wherein the mass of the part A is 20Kg as that of the yam micromolecule peptide; the mass of the part B is 10Kg as that of the lentinan, and the mass of the part C is 30Kg after subtracting the sum of the mass of the part A and the mass of the part B from the mass of the puffed rice flour in the step (1);

(3) airflow mixing the yam small molecular peptide and the part A of puffed rice flour for 20min to obtain a mixture I; airflow mixing Lentinus edodes glycopeptide and part B of puffed rice flour for 20min to obtain mixture II; and (3) mixing the algae oil powder and the C part puffed rice flour for 10min in an air flow manner to obtain a mixture III, adding the mixture I and the mixture II into the mixture III, continuing to mix for 25min in an air flow manner, and drying to obtain the composition for inducing the apoptosis of the tumor cells.

And in the air flow mixing process, starting a stirring paddle, wherein the speed of the stirring paddle is 6r/s, and simultaneously blowing air with the temperature of 25 ℃, the relative humidity of 85% and the air speed of 7m/s to boil the materials.

The drying is as follows: air with the temperature of 60 ℃ and the relative humidity of 25 percent is introduced for 8 minutes at the wind speed of 10 m/s.

Example 5

The preparation method of the composition for inducing the tumor cell apoptosis comprises the following steps:

(1) weighing 10Kg of yam micromolecule peptide according to the mass; 10Kg of lentinan; 10Kg of algae oil powder; puffed rice flour 70Kg (composition of example 2);

(2) dividing the puffed rice flour into three parts A, B and C according to the mass, wherein the mass of the part A is 10Kg as that of the yam micromolecule peptide; the mass of the part B is 10Kg as that of the lentinan, and the mass of the part C is 50Kg after subtracting the sum of the mass of the part A and the mass of the part B from the mass of the puffed rice flour in the step (1);

(3) airflow mixing the yam small molecular peptide and the part A of puffed rice flour for 15min to obtain a mixture I; airflow mixing Lentinus edodes glycopeptide and part B of puffed rice flour for 15min to obtain mixture II; and (3) mixing the algae oil powder and the C part puffed rice flour for 5min in an air flow manner to obtain a mixture III, adding the mixture I and the mixture II into the mixture III, continuing to mix for 20min in an air flow manner, and drying to obtain the composition for inducing tumor cell apoptosis.

And in the air flow mixing process, starting a stirring paddle, blowing air with the temperature of 20 ℃, the relative humidity of 80% and the air speed of 7m/s into the stirring paddle at the speed of 6r/s so as to boil the materials.

The drying is as follows: air with the temperature of 60 ℃ and the relative humidity of 20 percent is introduced for 5 minutes at the wind speed of 10 m/s.

Example 6

The preparation method of the composition for inducing the tumor cell apoptosis comprises the following steps:

(1) weighing 15Kg of yam micromolecule peptide; 5Kg of lentinan; 10Kg of algae oil powder; puffed rice flour 70Kg (composition of example 3);

(2) dividing the puffed rice flour into three parts A, B and C according to the mass, wherein the mass of the part A is 15Kg as that of the yam micromolecule peptide; the mass of the part B is 5Kg as that of the lentinan, and the mass of the part C is 50Kg after subtracting the sum of the mass of the part A and the mass of the part B from the mass of the puffed rice flour in the step (1);

(3) airflow mixing the yam small molecular peptide and the part A of puffed rice flour for 25min to obtain a mixture I; airflow mixing Lentinus edodes glycopeptide and part B puffed rice flour for 25min to obtain mixture II; and (3) mixing the algae oil powder and the C part puffed rice flour for 15min in an air flow manner to obtain a mixture III, adding the mixture I and the mixture II into the mixture III, continuing mixing for 30min in an air flow manner, and drying to obtain the composition for inducing tumor cell apoptosis.

And in the air flow mixing process, starting a stirring paddle, blowing air with the temperature of 30 ℃, the relative humidity of 90% and the air speed of 7m/s into the stirring paddle at the speed of 6r/s so as to boil the materials.

The drying is as follows: air with the temperature of 60 ℃ and the relative humidity of 30 percent is introduced for 10 minutes at the wind speed of 10 m/s.

Test example 1

Animal modeling, grouping and administration:

80 mice (SPF grade, female mice, 18-22g, 5-6 weeks) were randomly divided into model group, blank group (normal group), experimental group including lentinan peptide group, yam small molecule peptide group, algae oil powder group, example 4 group, example 5 group, example 6 group. 10/group, weighed and recorded. U14 cells (from Tokyo (Shanghai) Biotech Co., Ltd., source: Chinese medical academy of sciences) growing to logarithmic phase were inoculated subcutaneously to axilla of right forelimb⁵/mL)2mL, 1 time/d, 1g/Kg, 14d total administration by intragastric administration on the day after inoculation; the model group mice were administered 1ml/Kg of physiological saline by gastric gavage.

Weighing the mass 24h after the last administration, killing the mice by cervical dislocation, taking and weighing the tumor mass according to the following formula

Tumor inhibition rate,% (test group/model group tumor mass)

And respectively calculating the tumor inhibition rate. The processing adopts SPSS statistical software to process data for dataThe mean value comparison among the groups adopts one-factor analysis of variance, and the difference with P less than 0.05 has statistical significance.

TABLE 1 Effect on the body weight and tumor weight of cervical carcinoma U14 tumor-bearing mice (n ═ 10)

Test group Classification	Body weight, g before administration	Body weight, g after administration	Tumor mass, mg	Tumor inhibition rate%
					Model set	16.56±2.87	/	107±8.42	/
Blank group	23.43±2.56	23.43±2.56	/	/
					Lentinus edodes glycopeptide	16.56±2.87	18.45±2.13	92.34±8.36*	15
Chinese yam small molecular peptide	16.56±2.87	18.37±2.41	89.34±8.42*	17
					Algae oil powder	16.56±2.87	18.19±1.37	96.34±8.37*	11
Example 4	16.56±2.87	21.75±2.13*	45.74±5.19**	58
					Example 5	16.56±2.87	21.12±1.71*	50.38±4.34**	47
Example 6	16.56±2.87	21.05±2.42*	56.23±3.25**	48

Note: p <0.05, P <0.01,

through the test example 1, it can be seen that the influence of the raw material group (lentinan, yam small molecular peptide and algae oil powder) and the example group (example 4, example 5 and example 6) on the tumor mass content of the cervical cancer U14 tumor-bearing mice is different, and the tumor mass can be reduced (P < 0.05).

The weight of the raw material group (lentinan, yam micromolecular peptide and algae oil powder) has no significant difference relative to the weight of the model group. The results show that the weight of the mice is not improved, the malnutrition of the mice cannot be improved, and the weight of the mice is increased after the administration of the example groups (example 4, example 5 and example 6), and the differences of the weight of the mice relative to the model group are significant (P < 0.05). It is demonstrated that the group of examples (example 4, example 5, and example 6) can increase the body weight after administration and improve the malnutrition of mice. The formula food of the invention is superior to a single raw material, and has the effects of promoting tissue regeneration, improving the malnutrition of tumor patients and increasing the weight.

Test example 2

Animal modeling, grouping and administration: 50 mice (SPF grade, female mice, average body weight 18-22g, 5-6 weeks) were randomly assigned to model group, blank group (normal group), example 4 group, example 5 group, example 6 group, 10/group. Model and example groups a suspension of U14 cells (5 × 10) grown to logarithmic growth phase was inoculated subcutaneously in the axilla of the right forelimb⁵mL)2mL (from tokyo (shanghai) biotechnology limited, source: chinese academy of medical sciences), the groups of the examples and the inoculation are administered by intragastric administration for 1 time/d and 1g/Kg for 14 d; the model group mice were administered 1ml/Kg of physiological saline by gastric gavage.

Removing eyeball and blood 24 hr after last administration; killing mice by cervical dislocation, taking spleen and thymus, taking blood, centrifuging blood, separating serum, and detecting INF-gamma, TNF-alpha and IL-12 content according to the operation steps in the kit specification. The processing adopts SPSS statistical software to process data for dataThe mean value comparison among the groups adopts one-factor analysis of variance, and the difference with P less than 0.05 has statistical significance.

TABLE 2 Effect on the levels of INF-. gamma., TNF-. alpha., IL-2 in the sera of mice bearing tumors of cervical carcinoma U14 (n. 10)

Note: p <0.05, P <0.01,

as can be seen from the table 2, the groups in example 4 enhance the constitution of the mice, improve the blood indexes INF-gamma, TNF-alpha and IL-2 of the serum of the cervical cancer U14 tumor-bearing mice, and have a significant difference P of less than 0.05.

Example 5 groups of mice enhance the body constitution, improve the blood indexes TNF-alpha and IL-2 of the blood serum of the tumor-bearing mice with cervical cancer U14, and the difference is obvious, wherein P is less than 0.05.

Example 6 groups of mice enhanced constitution, the main action mechanism of the mice enhanced the blood indexes INF-gamma and IL-2 of the blood serum of the mice with the tumor of cervical cancer U14, and the difference is significant, and the P is less than 0.05.

Interleukin 2(IL-2) has a powerful regulating effect on the immune system. TNF-alpha is a factor contained in serum and capable of killing some tumor cells or making in vivo tumor tissue undergo the process of blood necrosis, INF-gamma interferon is a high-effective antiviral biological active substance, and is a lymph factor with extensive immunoregulation action.

The composition of the invention is prepared into oral preparations which are beneficial to use, such as powder, granules, capsules, tablets and the like, and is used as anti-cancer food for preventing and treating cancers.