阅读说明：本技术 生物多样性启动载体的制备方法 (Preparation method of biodiversity initiation carrier ) 是由 龙龙 黄润梓 李燕平 胡金龙 于 2021-09-08 设计创作，主要内容包括：本发明涉及一种生物多样性启动载体的制备方法,通过将载体与缓释碳源混合,然后与微生物菌剂定植发酵,使得微生物菌剂能够有效与载体结合,菌群间协同性好,然后吸附原生活性淤泥及有益藻种制得生物多样性启动载体,投入恢复区域的沉入底泥表层后,能够持续地释放微生物、原生活性微型生物及有益藻种,能够起到改造底泥和降解污染物的作用,并快速构建完整的适合该恢复区域环境条件下生长的微型生物种群,从而能够改善微环境内生物多样性,强化物种丰度,建立水环境物质循环和能量循环的微生态平衡系统,为下一步整个恢复区域的生物多样性的自然回归提供良好环境条件和基础物种。(The invention relates to a preparation method of a biodiversity initiation carrier, which comprises the steps of mixing the carrier with a slow-release carbon source, then the microbial inoculum is planted and fermented with the microbial inoculum, so that the microbial inoculum can be effectively combined with a carrier, the cooperativity among floras is good, then absorbing the primary activated sludge and beneficial algae species to prepare a biodiversity starting carrier, putting the biodiversity starting carrier into the recovery area and sinking into the surface layer of the bottom sludge, can continuously release microorganisms, primary active microorganisms and beneficial algae species, can play the roles of improving bottom mud and degrading pollutants, and rapidly construct a complete microbial population suitable for growth under the environmental conditions of the recovery area, therefore, the biodiversity in the microenvironment can be improved, the species abundance is enhanced, a microecological balance system of water environment substance circulation and energy circulation is established, and good environmental conditions and basic species are provided for the natural regression of the biodiversity of the whole recovery area in the next step.)

1. A preparation method of a biodiversity initiation vector is characterized by comprising the following steps:

mixing and granulating the carrier and the slow-release carbon source to obtain a first composite carrier;

fermenting and culturing the microbial agent to obtain a seed solution;

mixing the first composite carrier and the seed solution for field planting fermentation, and drying to obtain a second composite carrier;

mixing and adsorbing the second composite carrier and the primary activated sludge to obtain a third composite carrier;

embedding beneficial algae seeds in the third composite carrier to obtain the biodiversity starting carrier.

2. The method of claim 1, wherein the vector comprises: at least one of zeolite, volcanic rock, biological bamboo charcoal and ceramsite.

3. The method of claim 1, wherein the microbial agent comprises: at least one of bacillus, nitrobacteria, yeast, lactic acid bacteria, actinomycetes, ammonia oxidizing bacteria, denitrifying bacteria, EM bacteria, enterococcus and photosynthetic bacteria.

4. The method for preparing the biodiversity activating vector according to claim 1, wherein the step of performing fermentation culture on the microbial agent to obtain a seed solution comprises:

under the condition of high-temperature sterilization, the microbial inoculum is inoculated in a liquid culture medium, and then the liquid culture medium is placed in a shaking table for constant-temperature fermentation culture, so that the seed liquid is obtained.

5. The method for preparing the biodiversity initiation vector according to claim 1, wherein the step of mixing the first composite vector and the seed solution for field planting fermentation and drying to obtain the second composite vector comprises the following steps:

mixing the volume of the seed solution and the mass ratio of the first composite carrier according to the ratio of 3:1-5, adding a planting fermentation culture medium, finally putting the mixture into a fermentation tank for planting fermentation for 1-5 days, and finally filtering and air-drying the mixture to obtain the second composite carrier.

6. The method for preparing the biodiversity activating vector according to claim 5, wherein the permanent planting fermentation medium is 10g/L brown sugar and 5g/L yeast extract, and the pH value is 6.0.

7. The method of preparing a biodiversity activating vector according to claim 1, wherein the method of treating the primary activated sludge comprises:

collecting bottom sludge and water in a recovery area, mixing the bottom sludge and the water according to the volume ratio of 1:5-50, and then placing the mixture in an aeration tank for aeration for 2-5 days to enrich active microorganisms to obtain fermented bottom sludge;

detecting the biochemical oxygen demand and the ratio of nitrogen to phosphorus of the fermentation substrate sludge, supplementing a carbon source, a nitrogen source and a phosphorus source according to the ratio of the biochemical oxygen demand to the nitrogen to the phosphorus of 100:5:1, continuing aeration for 1-3 days, and repeating the operation for 1-3 times to obtain the primary activated sludge.

8. The method for preparing the biodiversity activating vector according to claim 1, wherein the biodiversity activating vector comprises the following components in parts by mass:

9. the method of claim 1, wherein the embedding of beneficial algal species within the third composite carrier further comprises:

and uniformly mixing the third composite carrier embedded with the beneficial algae seeds and the adhesive slow-release agent, and then granulating to obtain the biodiversity starting carrier.

10. The method for preparing the biodiversity activating carrier according to claim 9, wherein the third composite carrier embedding the beneficial alga species is 30 to 80 parts by mass, and the adhesive sustained-release agent is 1 to 20 parts by mass.

Technical Field

The invention relates to the technical field of water environment biodiversity protection and reconstruction, in particular to a preparation method of a biodiversity starting carrier.

Background

The existing treatment on black and odorous water is mainly to cut off dirt and remove silt, although the treatment can be immediately and quickly taken, because the water loses the self-purification capacity, the black and odorous return cannot be avoided, and the maintenance cost is high and can not be continued.

The microorganism is a natural decomposer, plays a fundamental core role in the ecological cycle of the earth environment, plays a role of a final decomposer, and directly throws the high-efficiency microorganism into the black and odorous water body, so that a good effect can be obtained in a short period.

Disclosure of Invention

Based on this, there is a need for a method for preparing a biodiversity priming vector.

The technical scheme for solving the technical problems is as follows: a preparation method of a biodiversity initiation vector comprises the following steps:

mixing and granulating the carrier and the slow-release carbon source to obtain a first composite carrier;

fermenting and culturing the microbial agent to obtain a seed solution;

mixing the first composite carrier and the seed solution for field planting fermentation, and drying to obtain a second composite carrier;

mixing and adsorbing the second composite carrier and the primary activated sludge to obtain a third composite carrier;

embedding beneficial algae seeds in the third composite carrier to obtain the biodiversity starting carrier.

In one embodiment, the carrier comprises: at least one of zeolite, volcanic rock, biological bamboo charcoal and ceramsite.

In one embodiment, the microbial agent comprises: at least one of bacillus, nitrobacteria, yeast, lactic acid bacteria, actinomycetes, ammonia oxidizing bacteria, denitrifying bacteria, EM bacteria, enterococcus and photosynthetic bacteria.

In one embodiment, the performing fermentation culture on the microbial agent to obtain a seed solution includes:

under the condition of high-temperature sterilization, the microbial inoculum is inoculated in a liquid culture medium, and then the liquid culture medium is placed in a shaking table for constant-temperature fermentation culture, so that the seed liquid is obtained.

In one embodiment, the mixing the first composite carrier and the seed solution for field planting fermentation, and drying to obtain the second composite carrier includes:

mixing the volume of the seed solution and the mass ratio of the first composite carrier according to the ratio of 3:1-5, adding a planting fermentation culture medium, finally putting the mixture into a fermentation tank for planting fermentation for 1-5 days, and finally filtering and air-drying the mixture to obtain the second composite carrier.

In one embodiment, the permanent planting fermentation medium comprises 10g/L of brown sugar and 5g/L of yeast extract, and the pH value is 6.0.

In one embodiment, the method of treating primary activated sludge comprises:

collecting bottom sludge and water in a recovery area, mixing the bottom sludge and the water according to the volume ratio of 1:5-50, and then placing the mixture in an aeration tank for aeration for 2-5 days to enrich active microorganisms to obtain fermented bottom sludge;

detecting the biochemical oxygen demand and the ratio of nitrogen to phosphorus of the fermentation substrate sludge, supplementing a carbon source, a nitrogen source and a phosphorus source according to the ratio of the biochemical oxygen demand to the nitrogen to the phosphorus of 100:5:1, continuing aeration for 1-3 days, and repeating the operation for 1-3 times to obtain the primary activated sludge.

In one embodiment, the biodiversity initiating vehicle comprises the following components in parts by mass:

in one embodiment, after embedding the beneficial algal species in the third composite carrier, the method further comprises:

and uniformly mixing the third composite carrier embedded with the beneficial algae seeds and the adhesive slow-release agent, and then granulating to obtain the biodiversity starting carrier.

In one embodiment, the third composite carrier embedding the beneficial algae is 30-80 parts by weight, and the adhesive sustained-release agent is 1-20 parts by weight.

The invention has the beneficial effects that: the invention provides a preparation method of a biodiversity initiation carrier, which comprises the steps of mixing the carrier with a slow-release carbon source, then the microbial inoculum is planted and fermented with the microbial inoculum, so that the microbial inoculum can be effectively combined with a carrier, the cooperativity among floras is good, then absorbing the primary activated sludge and beneficial algae species to prepare a biodiversity starting carrier, putting the biodiversity starting carrier into the recovery area and sinking into the surface layer of the bottom sludge, can continuously release microorganisms, primary active microorganisms and beneficial algae species, can play the roles of improving bottom mud and degrading pollutants, and rapidly construct a complete microbial population suitable for growth under the environmental conditions of the recovery area, therefore, the biodiversity in the microenvironment can be improved, the species abundance is enhanced, a microecological balance system of water environment substance circulation and energy circulation is established, and good environmental conditions and basic species are provided for the natural regression of the biodiversity of the whole recovery area in the next step.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings that are required to be used in the embodiments will be briefly described below, it should be understood that the following drawings only illustrate some embodiments of the present application and therefore should not be considered as limiting the scope, and for those skilled in the art, other related drawings can be obtained from the drawings without inventive effort.

FIG. 1 is a schematic flow chart of a method for preparing a biodiversity priming vector according to an embodiment of the present invention.

Detailed Description

To facilitate an understanding of the invention, the invention will now be described more fully with reference to the accompanying drawings. Preferred embodiments of the present invention are shown in the drawings. This invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete.

It will be understood that when an element is referred to as being "secured to" another element, it can be directly on the other element or intervening elements may also be present. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present. The terms "vertical," "horizontal," "left," "right," and the like as used herein are for illustrative purposes only and do not represent the only embodiments.

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used herein in the description of the invention is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. As used herein, the term "and/or" includes any and all combinations of one or more of the associated listed items.

In one embodiment, as shown in FIG. 1, a method for preparing a biodiversity priming vector comprises the steps of:

and 110, mixing and granulating the carrier and the slow-release carbon source to obtain a first composite carrier.

Specifically, the carrier includes: the slow-release carbon source can be modified slow-release starch, the carrier and the slow-release carbon source are mixed and granulated, and the slow-release carbon source is attached to the porous structure of the carrier, so that the internal condition of the porous structure of the carrier can be changed, energy, nutrient salt, vitamins and trace elements can be provided for biological endogenous metabolism, and a proper environment is provided for subsequent field planting of a microbial agent.

And step 120, performing fermentation culture on the microbial agent to obtain a seed solution.

Specifically, the microbial agent comprises: the bacillus can be one or more of bacillus subtilis, bacillus licheniformis, bacillus pumilus, bacillus cereus and nitrobacter winogradskyi, the yeast can be at least one of marine yeast and candida, the enterococcus can be at least one of enterococcus faecalis and enterococcus faecium, the lactic acid bacteria can be at least one of lactobacillus plantarum and lactobacillus lactis, the actinomycetes can be streptomyces, and the bacillus has the effects of killing or inhibiting microbes in water bodies, inhibiting blue algae and degrading toxic and harmful substances, improving bottom mud, purifying water quality and treating heavy metal pollution and improving the micro-ecological environment of the water bodies.

In this example, a liquid medium was prepared from 5g/L glucose, 3g/L peptone, 2g/L yeast extract, 1g/L sodium chloride, 0.25g/L magnesium chloride and 0.5g/L potassium dihydrogen phosphate, the pH of the liquid medium was adjusted to 6.5 to obtain the liquid medium, the microbial inoculum was inoculated into the liquid medium under high-temperature sterilization, the liquid medium was finally placed in a shaker at a culture temperature of 28 ℃ to 30 ℃ for constant-temperature fermentation culture for 30h to 60h to obtain a seed solution of the microbial inoculum, and the seed solution of the microbial inoculum was inoculated into the liquid medium again for proliferation culture to obtain a seed solution of the microbial inoculum after expanded culture.

And step 130, mixing the first composite carrier and the seed liquid for field planting fermentation, and drying to obtain a second composite carrier.

Specifically, a planting fermentation culture medium is prepared according to the raw material ratio of 10g/L brown sugar and 5g/L yeast extract, the pH value of the planting fermentation culture medium is adjusted to 6.0, the seed solution is mixed according to the mass ratio of 3:1-5 with the first composite carrier, then the prepared planting fermentation culture medium is added, finally the planting fermentation culture medium and the prepared planting fermentation culture medium are placed into a fermentation tank together, planting fermentation is carried out for 1-5 days under the culture conditions that the culture temperature is 28-30 ℃ and the stirring rate is 100r/min, and finally filtering and air drying are carried out to obtain a second composite carrier.

In this embodiment, prepare the second composite carrier through the seed solution of microbial inoculum and the first composite carrier field planting fermentation, the compatibility between microorganism and the carrier has been improved, the internal environment of microorganism adaptation carrier, make the growth and reproduction of microorganism coordinate with the internal environment of carrier mutually, thus, microorganism on the second composite carrier can combine with the carrier effectively, the ratio between flora quantity and each flora is more stable, and single thallus vigor is higher, have more efficient synergism between each flora, after putting into the recovery area, can continuously release microorganism to water and mud floating layer, reform transform and degrade the pollutant to the bed mud, the slow-release microorganism biomass is big, it is better to the environmental modification effect.

And 140, mixing and adsorbing the second composite carrier and the primary activated sludge to obtain a third composite carrier.

Specifically, the method for treating the primary activated sludge comprises the steps of collecting bottom sludge and water in a recovery area, mixing the bottom sludge and the water according to the volume ratio of 1:5-50, placing the mixture in an aeration tank, aerating for 2-5 days to enrich active microorganisms to obtain fermented bottom sludge, detecting the biochemical oxygen demand and the nitrogen-phosphorus ratio of the fermented bottom sludge, supplementing a carbon source, a nitrogen source and a phosphorus source according to the biochemical oxygen demand and nitrogen-phosphorus ratio of 100:5:1, continuing aerating for 1-3 days, and repeating the operation for 1-3 times to obtain the primary activated sludge.

In this embodiment, the second composite carrier and the primary activated sludge are mixed to obtain the carrier of the primary microorganisms with the recovery area, after the carrier is thrown into the recovery area, the primary microorganisms are gradually released, the adaptability is strong, a complete microbial population suitable for the growth of the recovery area can be quickly constructed, the environment is further improved, and the ecological balance of the water body microenvironment is perfected.

And 150, embedding the beneficial algae in the third composite carrier to obtain the biodiversity starting carrier.

Specifically, beneficial algae seeds are embedded in the third composite carrier, so that after the carrier is thrown into a recovery area, the beneficial algae seeds can absorb nutrient salts converted when the microbial agent transforms bottom mud and a water body, photosynthesis is carried out by absorbing solar energy to synthesize energy for growth and propagation, meanwhile, the energy is provided for a micro-ecological system, a complete and closed water ecological microenvironment is established, and the micro-ecological balance system with water environmental substance circulation and energy circulation is obtained.

In one embodiment, the biodiversity initiating vehicle comprises the following components in parts by mass:

the invention provides a preparation method of a biodiversity starting carrier, which comprises the steps of organically combining a carrier, a slow-release carbon source, a microbial agent, primary activated sludge, beneficial algae species and an embedding medium to obtain the biodiversity starting carrier, putting the biodiversity starting carrier into a water body in a recovery area, sinking the biodiversity starting carrier into the surface layer of bottom sludge, continuously releasing microorganisms, primary active microorganisms and beneficial algae to the recovery area, leading the microorganisms to play a role in modifying the bottom sludge and degrading pollutants, quickly constructing a complete microbial population suitable for growing in the recovery area under the environmental condition through the primary active microorganisms, further perfecting the ecological balance of the water body, matching with the beneficial algae species to absorb nutrient salts in the bottom sludge and the water body, carrying out photosynthesis through absorbing solar energy to provide energy for a micro ecological system, and further quickly establishing a micro microbial population based on the beneficial microbial population, with the assistance of activated sludge micro organisms, beneficial algae species absorb solar energy to carry out photosynthesis to supplement energy to form a complete closed water ecological microenvironment, so that a micro ecological balance system with water environmental substance circulation and energy circulation is obtained, and good environmental conditions and basic species are provided for natural regression of biodiversity of the whole recovery area in the next step.

In one embodiment, after the beneficial algae species are embedded in the third composite carrier, the method further comprises the steps of uniformly mixing the beneficial algae species embedded third composite carrier with the adhesive sustained-release agent, and then granulating to obtain the biodiversity initiating carrier. Specifically, the beneficial alga species-embedded third composite carrier is prepared from 30-80 parts by mass and 1-20 parts by mass of the adhesive slow-release agent through mixing and granulating according to the proportion, so that when the biodiversity starting carrier is put into water, the microbial, the protogenic active microorganisms and the beneficial alga are slowly decomposed through the adhesive slow-release agent and are gradually released to act on a recovery area, and meanwhile, starch of a dissolving part of the adhesive slow-release agent forms a new carbon source, so that energy can be provided for the microorganisms, and the aquatic microenvironment of the recovery area can be continuously modified.

The invention is further described below with specific embodiments.

The shallow Australian river is an artificial flood diversion river entering the sea, is positioned in a great Asia bay area of Huizhou city, Guangdong province, has the characteristics of emergent water flow, large water area, large tidal and rainy season water level drop and alternation of saline water and has the main indexes of successfully reaching four standards of national surface water after years of treatment, and the treatment section is in a bell mouth shape, the downstream width exceeds 200 meters, and the upstream width is less than 30 meters. Through detection, in the midstream of the Weak Australian river, the number of upstream crabs is small, the damage of habitat and environment is not completely recovered, and the loss of biological chains is serious.

When starting biological diversity recovery for the river basin of the Weak Australia, selecting an upstream sweet osmanthus bridge area, a midstream river beach park area and a downstream Cangzhou aigret area as biological diversity recovery starting points, taking the three starting points of the upstream, the midstream and the downstream as throwing areas of biological diversity starting carriers, and selecting three key points at a river course slow flow position to facilitate starting biological diversity recovery work.

After the water is put for six months, the water quality of the starting point of biological diversity recovery is measured, the ammonia nitrogen content of the upper, middle and lower starting points is lower than 1.5mg/L, the phosphorus content is lower than 0.3mg/L, the chemical oxygen demand is lower than 30mg/L, the heavy metal copper ion content is lower than 0.5mg/L, and the water body with the heavy metal lead ion content of lower than 0.02mg/L is clear, transparent and tasteless. The microbial bed is established, a large number of organisms suitable for the whole environment multiply and live, benthic algae are increased obviously, plankton is enriched obviously, the abundance of various typical species organisms is greatly improved, data analysis shows that the minimum promotion proportion of the plankton to the benthic organisms is 15 percent and the maximum promotion proportion is 1000 percent, and the light Australian river establishes a rudiment of energy flow, material flow and information flow circulation, so that good environmental conditions and basic species are provided for the natural regression of the biodiversity of the whole recovery area in the next step.

The technical features of the embodiments described above may be arbitrarily combined, and for the sake of brevity, all possible combinations of the technical features in the embodiments described above are not described, but should be considered as being within the scope of the present specification as long as there is no contradiction between the combinations of the technical features.

The above embodiments only express a few embodiments of the present application, and the description thereof is more specific and detailed, but not construed as limiting the scope of the invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the concept of the present application, which falls within the scope of protection of the present application. Therefore, the protection scope of the present patent shall be subject to the appended claims.