阅读说明：本技术 一种胶原蛋白肽组合物及其制备方法 (Collagen peptide composition and preparation method thereof ) 是由 贾振华 吴相君 吴瑞 刘京 张璐 李锦� 张胜楠 郭晓玉 于 2020-06-03 设计创作，主要内容包括：本发明提供了一种胶原蛋白肽组合物及其制备方法。该组合物由胶原蛋白肽及红石榴粉等主要成分组成,通过合理的配伍和科学的制备,香气宜人,味道可口,深受广大试用人群的好评,方便携带,能有效的实现美白保湿的效果,值得推广应用。(The invention provides a collagen peptide composition and a preparation method thereof. The composition comprises main components such as collagen peptide and pomegranate powder, is pleasant in fragrance and delicious in taste through reasonable compatibility and scientific preparation, is deeply favored by general trial crowds, is convenient to carry, can effectively realize the effects of whitening and moisturizing, and is worthy of popularization and application.)

1. The collagen peptide composition is characterized by comprising the following raw materials in parts by weight: 60-90 parts of fish collagen peptide; 8-40 parts of pomegranate juice powder; 1-3 parts of yeast extract.

2. The composition according to claim 1, characterized in that it consists of the following raw materials in parts by weight: 60 parts of fish collagen peptide; 38 parts of pomegranate juice powder; and 2 parts of yeast extract.

3. The composition according to claim 1, characterized in that it consists of the following raw materials in parts by weight: 90 parts of fish collagen peptide; 9 parts of pomegranate juice powder; 1 part of yeast extract.

4. The composition according to claim 1, which is prepared from the following raw materials in parts by weight: 83.34 parts of fish collagen peptide; 15 parts of pomegranate juice powder; 1.66 parts of yeast extract.

5. The composition according to any one of claims 1-4, wherein the fish collagen peptides are a blend of fish collagen peptides of the two specifications NLC-500-G and P-FC04, and the pomegranate juice powder is a blend of pomegranate fruit powders of the two specifications DSL-1 and BS 25-1.

6. The composition according to claim 5, characterized in that it consists of the following raw materials in parts by weight: 41.67 parts of NLC-500-G fish collagen peptide powder, 41.67 parts of P-FC04 fish collagen peptide powder, 12 parts of DSL-1 red pomegranate fruit juice powder, 3 parts of BS25-1 red pomegranate fruit juice powder and 1.66 parts of yeast extract.

7. The composition according to any one of claims 1 to 4, wherein the composition is formulated as a solid beverage, soft drink, mixed powder, rice milk, milk shake, capsule or tablet.

8. Composition according to claim 7, characterized in that the solid beverage product process is:

a. weighing: respectively weighing raw and auxiliary materials including fish collagen peptide powder NLC-500-G, fish collagen peptide powder P-FC04, pomegranate fruit juice powder DSL-1, pomegranate fruit juice powder BS25-1 and yeast extract for later use;

b. mixing: mixing weighed pomegranate fruit juice powder BS25-1 and yeast extract for 5min, mixing with pomegranate fruit juice powder DSL-1 for 10min, mixing with fish collagen peptide powder NLC-500-G and fish collagen peptide powder P-FC04 for 30 min until the color is uniform and consistent to obtain total mixed powder;

c. packaging, inspecting, and warehousing.

Technical Field

The invention relates to a collagen peptide composition and a preparation method thereof, in particular to a composition with the effects of increasing skin elasticity, moisturizing and whitening skin.

Background

With the increasing living standard of people, the pursuit of beauty for people is rapidly improved. The skin itself is aged with the age, and in addition, the health of the skin is seriously affected by smoking, drinking, staying up all night and the like in daily life, so that various problems occur to the skin, and negative images are caused to the body and the mind.

Good skin can make people more confident, thereby changing the mind of the people. According to data obtained by network investigation, Chinese people in the world desire to improve a series of problems such as dull aging, lack of elasticity and the like of own skin by various ways, not only women, but also men. The greatest demand for improvement of skin is whitening, and other demands accompanying whitening are ranked as follows: 33% hoped to simultaneously combat skin aging, 28.5% hoped to simultaneously lighten skin tone, 21.5% hoped to simultaneously lighten existing spots, and 17% hoped to simultaneously be more moist. Therefore, the skin care effect is mixed with whitening, and the skin care product is more and more popular with women.

Human skin tissue is rich in more than 50% of collagen, maintains the basic structure of the skin in the form of collagen fibers and elastic fibers, and plays an important role in the moisture, elasticity and texture of the skin. However, with aging, collagen in human body is gradually lost in large quantity, which is the most main reason for skin aging. Skin aging can cause a series of negative reactions such as skin darkness, dryness and the like, so that the skin can be kept young by continuously supplementing collagen.

Various facial masks and creams can moisturize water and keep moisture because the facial masks and creams can moisturize the horny layer of the skin and moisturize and lock the horny layer at the outermost layer of the skin. In the case of dry atrophy of the stratum corneum, the problem of severe skin dryness due to water loss occurs. At this time, the skin care product with strong water replenishing function is used, and the water shortage condition of the skin can be improved after the skin care product is used for a plurality of days. However, the effect is very little for the overall improvement, so that the requirements of skin whitening and moisturizing can not be met only by the external product, and the skin can be helped to be recovered to the best state by combining the related beauty products and good daily life habits while the external product is used.

To make the collagen ingested by oral administration easier to be digested and absorbed by human body, manufacturers need to optimize the process design of the collagen continuously and improve the production level of the collagen to achieve the effective absorption of the collagen by human body. Various collagen products are mixed in the current market, most enterprises use the condition that the molecular weight range of national industry standard is too large, large molecular weight collagen which is not easy to be absorbed by human bodies flows into the market, and bad images are caused to consumers. And the collagen can only improve the skin elasticity, and can not improve the skin in all directions.

China is the biggest fish culture country in the world, and the fish scale resources are quite rich, so that the problem of environmental pollution can be solved by reasonably utilizing the fish scales. The structure of collagen and its hydrolysate is similar to that of collagen on human skin, and is rich in amino acids and hydroxyl radicals, so that collagen is used as the basis of beautifying. Collagen peptide (collagen peptide for short) is protein with smaller molecular weight than collagen, and has stronger digestion and absorption, thereby being beneficial to the digestion and absorption of human small intestine.

Pomegranate powder is a cosmetic product that has been widely accepted by the market in recent years. Has the following functions: 1) Antioxidation: punicalagin and ellagic acid in the pomegranate condensed powder can greatly improve the oxidation resistance of blood, and ellagic acid is an antioxidant which can improve the oxidation resistance of blood; through experimental control, the pomegranate concentrated powder can obviously slow down protein and lipid peroxidation; 2) Resisting: the fibroblast irradiated by ultraviolet light and blue light is effectively protected, and death sources of the fibroblast are reduced; reducing the expression of MMP1, MMP13, and caspase, thereby preventing zhidao degradation of collagen in skin and reducing apoptosis of cells after blue light irradiation; 3) Blocking the pigmentation process: ellagic acid in punica granatum can effectively inhibit tyrosinase activity, thereby blocking generation of melanin after sunburn.

Similar products are available on the market, but the effects are different because of different proportions. The inventor provides a verified composition with reasonable proportion, and experiments prove that the composition has very good beautifying effect and good and praised mouthfeel, and is a skin beautifying product which is urgently needed in the market.

Disclosure of Invention

The invention provides a composition, which is prepared by taking fish collagen peptide powder as a main raw material and combining pomegranate juice powder and yeast extract. The composition obtained by researching the wild AB strain zebra fish has obvious effects on skin elasticity, water replenishing, moisture preserving and whitening.

The composition is prepared by mixing a plurality of components, and no auxiliary materials or additives are added in the processing process, so that the nutritional ingredients of the raw materials can be completely reserved, and the characteristics of rich flavor, rich nutrition, fine and smooth mouthfeel, easiness in brewing and stable product state are achieved. The product can be mixed with cold water, warm water or boiled water, and can be eaten directly without hot water.

The composition has a simple preparation process and scientific formula of each component, meets the requirements of target people on improving skin, achieves the effects of whitening, moisturizing, increasing skin elasticity and the like, and is suitable for popularization.

The composition of the invention comprises the following raw materials in parts by weight: 60-90 parts of collagen peptide; 8-40 parts of pomegranate juice powder; 1-3 parts of yeast extract.

Preferably, the composition of the invention consists of the following raw materials in parts by weight: 60 parts of collagen peptide; 38 parts of pomegranate juice powder; and 2 parts of yeast extract.

Preferably, the composition of the present invention may also be composed of the following raw materials in parts by weight: 90 parts of collagen peptide; 9 parts of pomegranate juice powder; 1 part of yeast extract.

More preferably, the composition of the invention can also consist of the following raw materials in parts by weight: 83.34 parts of collagen peptide; 15 parts of pomegranate juice powder; 1.66 parts of yeast extract.

The collagen peptide used in the composition of the present invention is a fish collagen peptide.

The fish collagen peptide used in the components of the invention is formed by mixing two fish collagen peptides of NLC-500-G and P-FC04 with the same amount.

The pomegranate juice in the composition of the invention is prepared by mixing the pomegranate juice powders of two specifications of DSL-1 and BS 25-1.

The composition of the invention preferably also comprises the following raw materials in parts by weight: 41.67 parts of NLC-500-G fish collagen peptide powder, 41.67 parts of P-FC04 fish collagen peptide powder, 12 parts of DSL-1 red pomegranate fruit juice powder, 3 parts of BS25-1 red pomegranate fruit juice powder and 1.66 parts of yeast extract.

The composition of the invention can be prepared into solid beverages, soft drinks, mixed powder, rice water, milk shake, capsules or tablets.

The composition of the present invention is preferably prepared as a solid beverage.

The product process for preparing the solid beverage comprises the following steps:

a. weighing: respectively weighing raw and auxiliary materials including fish collagen peptide powder NLC-500-G, fish collagen peptide powder P-FC04, pomegranate fruit juice powder DSL-1, pomegranate fruit juice powder BS25-1 and yeast extract for later use;

b. mixing: mixing weighed pomegranate fruit juice powder BS25-1 and yeast extract for 5min, mixing with pomegranate fruit juice powder DSL-1 for 10min, mixing with fish collagen peptide powder NLC-500-G and fish collagen peptide powder P-FC04 for 30 min until the color is uniform and consistent to obtain total mixed powder;

c. packaging, inspecting, and warehousing.

The composition is convenient to take and good in taste, and meets the requirements of target people on the effects of improving skin, increasing skin elasticity, whitening and moisturizing; and the storage period is long, thus being suitable for popularization in crowds.

In order to verify the mouthfeel properties and specific efficacy of the composition of the invention, the inventors carried out experiments using the solid beverage prepared in example 1 and comparative examples 1 to 7 with different component ratios, as follows:

test example 1: to characterize the properties of the solid beverages prepared by the method of the present invention, sensory evaluation tests were performed on the samples prepared in the examples, as well as the samples prepared in comparative examples 1 to 7, by a panel of 10 persons consisting of a professional. Carrying out comprehensive scoring according to the appearance, smell, color, solubility and tissue state of the solid beverage, wherein the calculation method of the comprehensive scoring comprises the following steps: the full scores of the five investigation indexes are all 100, the weight occupied in the evaluation system is 20%, each index score is multiplied by the proportion of the occupied weight, the scores are added to obtain a comprehensive score, and the specific results are shown in the following table 1:

as can be seen from table 1, comparative examples 2, 6 and examples all scored significantly higher than the other samples.

In order to investigate the efficacy of the composition, the composition is specially adopted to evaluate the efficacy of the wild AB strain zebra fish on skin elasticity, moisturizing and whitening.

Main apparatus and reagents: dissecting microscope (SZX 7, OLYMPUS, Japan), camera connected to microscope (VertA 1, shanghai saton vision science and technology limited, china); a micro-spectrophotometer (NanoDrop 2000, Thermo, America); general PCR amplificators (T100, Bio-rad, Singapore); fluorescent quantitative PCR instrument (CFX Connect, Bio-rad, Singapore); precision electronic balances (CP 214, OHAUS, China); 6-well plates (Fisher scientific, China); low skirt 96-well plates (clear) (HSP 9601, Bio-rad, America); optical adhesive seal B (MSB 1001, Bio-rad, America).

Isopropanol (cat No. a507048, shanghai bio); chloroform (cat. No. T130144, alatin reagent (shanghai) ltd.); dimethyl sulfoxide (DMSO, lot No. SHBH6853, Sigma); absolute ethanol (batch No. 20181210, kunshan jincheng reagent limited); methylcellulose (batch No. 079K0054V, Sigma, USA); iTaq Universal SYBR Green Supermix (1725121, Bio-rad, America); FastQuant RT Kit (With gDNase) Kit (cat # KR106, Tiangen); TRI reagent (cat # 1382739, nvitrogen); bradford 1x Dye Reagent (# 500-; bone Serum Albumin (BSA) Standard (# 500-; RIPA lysate (P0013B, Beyotime); 100 mM PMSF (ST 506-2, Beyotime), L-levodopa (I1705022, Aladdin).

Sodium chloride, white crystals, batch No. 20180803, available from shanghai, u.s.a.test; arbutin, white powder, lot 35648, available from Aladdin reagent (Shanghai) Co., Ltd.

Experimental methods

To identify the efficacy of the compositions of the present invention, efficacy evaluation experiments were conducted on the samples prepared in the examples, as well as the samples prepared in comparative examples 1 to 7. The examples are prepared into three high, medium and low concentration aqueous solutions of 222, 667 and 2000 mug/mL, and the comparative samples are all prepared into 667 mug/mL aqueous solutions. The skin elasticity, the moisturizing effect and the whitening effect of each sample are examined by referring to the wild AB strain zebra fish, and the method specifically comprises the following steps:

1. influence on skin elasticity

Wild type AB line zebra fish 5 days after fertilization were randomly selected and placed in a six-well plate, and 30 zebra fish were treated per well (experimental group). The samples of examples and comparative examples were each dosed in water, while a normal control group was set, with a capacity of 3 mL per well. The experiments were performed in parallel for 3 times. After the test sample treatment is finished, the classical Trizol method is used for extracting the total RNA of each group of zebra fish, and the transcription level of the target gene col1a1b is detected by q-PCR. Beta-actin is used as an internal reference for gene expression, and the relative expression quantity of the RNA of the target gene is calculated. Statistical analysis using analysis of variance and Dunnett's T-test indicated significant differences with p < 0.05.

As can be seen from Table 2, after the zebra fish is treated by the test product, compared with the normal control group, the relative expression level of the skin elasticity col1a1b gene can be added in each dosage group, and the obvious difference (p < 0.01) is obtained, namely the solid beverage provided by the invention has an obvious effect of increasing the skin elasticity.

2. Moisturizing effect

Randomly selecting 2 days after fertilization, namely selecting the black pigment allele mutation translucent AL strain zebra fish in a six-hole plate, and treating 30 zebra fish in each hole (experimental group). And (3) dissolving in water and giving sodium chloride to establish a zebra fish micro-seepage water-shortage model. The samples of examples and comparative examples were each dosed in water, while a normal control group and a model control group were set with a capacity of 3 mL per well. After the treatment of the test article is finished, 10 zebra fish of each group are randomly taken and photographed under an anatomical microscope and pictures are stored, the tail area (A) of the zebra fish is analyzed by NIS-Elements D3.10 advanced image processing software, the water replenishing and moisturizing effects of the sample on the slightly water-deficient zebra fish are evaluated according to the statistical analysis result of the tail area, and the statistical treatment result is expressed by mean +/-SE. Statistical analysis using analysis of variance and Dunnett's T-test indicated significant differences with p < 0.05.

According to the experimental results, the composition solid beverage has the functions of supplementing water and moisturizing.

3. Evaluation of whitening Effect

Randomly selecting wild AB strain zebra fishes 6 hours after fertilization into a six-hole plate, treating 30 zebra fishes in each hole (experimental group), respectively dissolving the zebra fishes in water, and giving samples of examples and comparative examples, wherein the concentration of arbutin in a positive control group is 3000 mug/mL, and meanwhile, setting a normal control group (water for fish culture to treat the zebra fishes), wherein the volume of each hole is 3 mL. After each experimental group is treated for 48 hours, 10 zebra fish are randomly selected from each experimental group, photographed under an anatomical microscope and data are collected, Image analysis is carried out by Image J Image processing software, data are collected, the optical density sum of melanin at the head of the zebra fish is analyzed and counted, and the whitening effect of the test article on the zebra fish is evaluated according to the statistical analysis result of the optical density sum of the melanin.

As shown in Table 4, after the zebra fish is treated by the test product, compared with a normal control group, the total melanin optical density of each dose group is reduced, and the significant difference (p is less than 0.001) is obtained, namely the solid beverage of the composition has obvious whitening effect.

4. Inhibition of tyrosinase activity

Wild-type AB strain zebra fish 6 hours after fertilization are randomly selected to be placed in a six-well plate, 30 zebra fish are treated in each well (experimental group), samples of examples and comparative examples are given in a water-soluble mode, a positive control agent arbutin is 3000 mu g/mL, a normal control group (zebra fish treated by water for fish culture) is arranged at the same time, three groups are arranged in parallel in each group, and the capacity of each well is 3 mL. After each experimental group is treated for 48 hours, an absorbance value is measured at the wavelength of 475 nm by a dopamine oxidation method, each group is provided with 3 compound holes, and the inhibition effect of the test sample on the tyrosinase activity of the zebra fish is evaluated according to the statistical analysis result of the absorbance value.

As can be seen from Table 5, after the zebra fish is treated with the test product, compared with the normal control group, the activity of tyrosinase can be reduced in each dose group, and the significant difference (p < 0.001) is obtained, namely the solid beverage of the invention has an obvious inhibition effect on tyrosinase.

According to the test results, the solid beverage prepared by the formula and the preparation method of the solid beverage has the color and luster, good solubility, no peculiar smell in taste, moderate sweetness and sourness, the flavor of the solid beverage, the grade of sensory evaluation experts is more than 90 minutes, and the composition has obvious effects on skin elasticity, moisturizing, whitening and tyrosinase inhibition compared with single components. And the expert scores of the samples prepared in the comparative examples 1-7 (except for the comparative examples 2 and 6) are all below 80 points, which shows that the change of the proportion of the ingredients has great influence on the aspects of appearance, smell, color and the like of the solid beverage and directly influences the quality of the product. Comparative examples 2 and 6 have the highest scores in sensory evaluation, but have significantly lower skin elasticity, moisturizing, whitening effects, and tyrosinase inhibitory effects in efficacy evaluation than in examples. Therefore, by combining the results of the sensory evaluation and the efficacy evaluation, the dosage ratio of the raw materials to be protected by the invention is the optimal proportioning range obtained by a great deal of labor of the inventor.

Comparative examples 1 to 7: adjusting the types and the dosages of the fish collagen peptide powder and the pomegranate juice powder, wherein the specific dosages are shown in the following table:

Detailed Description

The present invention will be described in further detail below with reference to specific examples to assist understanding of the contents of the present invention.

Example 1

41.67G of NLC-500-G fish collagen peptide powder, 41.67G of P-FC04 fish collagen peptide powder, 12G of DSL-1 pomegranate fruit juice powder, 3G of BS25-1 pomegranate fruit juice powder and 1.66G of yeast extract

The preparation method comprises the following steps:

a. weighing: respectively weighing fish collagen peptide powder NLC-500-G, fish collagen peptide powder P-FC04, pomegranate fruit juice powder DSL-1, pomegranate fruit juice powder BS25-1 and yeast extract for later use;

b. mixing: mixing weighed pomegranate fruit juice powder BS25-1 and yeast extract for 5min, mixing with pomegranate fruit juice powder DSL-1 for 10min, mixing with fish collagen peptide powder NLC-500-G and fish collagen peptide powder P-FC04 for 30 min until the color is uniform and consistent to obtain total mixed powder;

c. packaging, inspecting, and warehousing.

Example 2

NLC-500-G fish collagen peptide powder 83.34G, DSL-1 red pomegranate fruit juice powder 12G, BS25-1 red pomegranate fruit juice powder 3G, yeast extract 1.66G

The preparation method comprises the following steps:

a. weighing: respectively weighing fish collagen peptide powder NLC-500-G, pomegranate fruit juice powder DSL-1, pomegranate fruit juice powder BS25-1 and yeast extract for later use;

b. mixing: mixing weighed pomegranate fruit juice powder BS25-1 and yeast extract for 5min, mixing with pomegranate fruit juice powder DSL-1 for 10min, and mixing with fish collagen peptide powder NLC-500-G for 30 min until the color is uniform to obtain total mixed powder;

c. packaging, inspecting, and warehousing.

Example 3

P-FC04 fish collagen peptide powder 83.34g, DSL-1 red pomegranate fruit juice powder 12g, BS25-1 red pomegranate fruit juice powder 3g, yeast extract 1.66g

The preparation method comprises the following steps:

a. weighing: respectively weighing fish collagen peptide powder P-FC04, pomegranate fruit juice powder DSL-1, pomegranate fruit juice powder BS25-1 and yeast extract for later use;

b. mixing: mixing weighed pomegranate fruit juice powder BS25-1 and yeast extract for 5min, mixing with pomegranate fruit juice powder DSL-1 for 10min, and mixing with fish collagen peptide powder P-FC04 for 30 min until the color is uniform to obtain total mixed powder;

c. packaging, inspecting, and warehousing.

Example 4

NLC-500-G fish collagen peptide powder 30G, P-FC04 fish collagen peptide powder 30G, DSL-1 red pomegranate fruit juice powder 25G, BS25-1 red pomegranate fruit juice powder 13G, yeast extract 2G

The preparation method comprises the following steps:

a. weighing: respectively weighing fish collagen peptide powder NLC-500-G, fish collagen peptide powder P-FC04, pomegranate fruit juice powder DSL-1, pomegranate fruit juice powder BS25-1 and yeast extract for later use;

b. mixing: mixing weighed pomegranate fruit juice powder BS25-1 and yeast extract for 5min, mixing with pomegranate fruit juice powder DSL-1 for 10min, mixing with fish collagen peptide powder NLC-500-G and fish collagen peptide powder P-FC04 for 30 min until the color is uniform and consistent to obtain total mixed powder;

c. packaging, inspecting, and warehousing.