阅读说明：本技术 一种具有解酒功效低聚果胶复合冲剂的制备方法 (Preparation method of pectin oligomer composite granules with anti-alcohol effect ) 是由 陈进林 陈文勇 莫树平 于 2021-09-06 设计创作，主要内容包括：本发明公开了一种具有解酒功效低聚果胶复合冲剂的制备方法,其原料中含有：低聚改性果胶、葛根、山楂、甘草、陈皮、谷胱甘肽、米糠脂肪烷醇、柠檬酸钾、柠檬酸。制备时,将原料经粉碎、萃取、混合、造粒、烘干即为成品。整个配方相互配伍,发挥协同增效作用,起到餐前开胃、防醉解酒、保护肠胃等效果。本发明剂型为颗粒冲剂,可以冲服成饮品、携带方便、便于保存,可用于所有饮酒过量者。(The invention discloses a preparation method of an oligo-pectin composite electuary with an anti-alcoholism effect, which comprises the following raw materials: oligomerization modified pectin, radix puerariae, hawthorn, liquorice, pericarpium citri reticulatae, glutathione, rice bran fatty alkanol, potassium citrate and citric acid. During preparation, the raw materials are crushed, extracted, mixed, granulated and dried to obtain a finished product. The whole formula is compatible with each other, plays a role in synergy, and has the effects of stimulating appetite before meals, preventing drunkenness and relieving alcoholism, protecting intestines and stomach and the like. The preparation is granule, can be taken as beverage, is convenient to carry and store, and can be used for all people who drink too much.)

1. The preparation method of the oligo pectin composite granules with the effect of relieving alcoholism is characterized by comprising the following steps:

(1) crushing: freezing and pulverizing radix Puerariae, fructus crataegi, Glycyrrhrizae radix, and pericarpium Citri Tangerinae to obtain powder with particle size of 30-200 μm;

(2) and (3) extraction: and (3) processing the crushed raw materials obtained in the step (1) by adopting a supercritical extraction technology to extract active ingredients in the materials.

(3) Adsorption: adding the extract obtained in the step (2) into an oligomerization modified pectin solution, stirring at 45-65 ℃ for 12-24h, and then freeze-drying the adsorption solution;

(4) and (3) granulation: and (4) uniformly mixing the oligo-pectin freeze-dried powder obtained in the step (3) with glutathione, rice bran fatty alkanol, potassium citrate and citric acid, adding a small amount of auxiliary materials, granulating, drying and crushing to obtain the composite electuary.

2. The method according to claim 1, wherein the cold pulverization temperature is from-150 ℃ to-90 ℃ in the step (1).

3. The preparation method according to claim 1, wherein in the step (1), the radix puerariae is added in an amount of 1-5 parts, 2-6 parts of hawthorn, 2-4 parts of liquorice and 2-5 parts of dried orange peel.

4. The preparation method according to claim 1, wherein in the step (2), the supercritical extraction technology pulverizes the raw material to 20-30% of the volume of the extraction kettle.

5. The method according to claim 1, wherein in the step (2), the flow rate of the nitrogen dioxide in the supercritical extraction process is 27 to 30 kg/h.

6. The method according to claim 1, wherein the supercritical extraction time in step (2) is 90-120 min.

7. The method according to claim 1, wherein in the step (3), the molecular weight of the oligo-modified pectin is 10 to 30 ku.

8. The preparation method according to claim 1, wherein in the step (3), the grafting ratio of the flavonoid compound in the oligo-modified pectin is not less than 100 mg/g.

9. The preparation method according to claim 1, wherein in the step (4), the addition amount of the freeze-dried low pectin powder is 5-10 parts, 2-5 parts of glutathione, 2-4 parts of rice bran fatty alkanol, 1-3 parts of potassium citrate and 1-3 parts of citric acid.

Technical Field

The invention relates to a preparation method of an oligo-pectin composite electuary with an anti-alcohol effect, and belongs to the technical field of foods.

Background

Chinese has a long history of wine culture, and drinking becomes an indispensable social mode for people. Acute alcoholism caused by a single large amount of drinking can cause acidosis, heart failure, autonomic nerve and brain dysfunction and even cause respiratory depression to die, however, with the improvement of living standard, the number of drinking people in China also increases year by year, and high attention should be paid to the prevention and treatment of acute alcoholism. Therefore, there is a need for an anti-hangover product that is effective, safe, non-toxic, and non-dependent. At present, the types of the dealcoholic products on the market are more, the general reaction effect is not obvious, and the protection of the gastrointestinal tract and the liver is not obvious while sobering.

Alcohol entering human body is absorbed mainly by stomach and small intestine, and is metabolized by alcohol dehydrogenase and acetaldehyde dehydrogenase to generate acetic acid. The metabolism induces the production of free radicals in vivo, and damages various organs such as liver. Researches show that the oligo-pectin can form a protective film in the gastrointestinal tract of a human body and can enter blood to promote alcohol decomposition, and the oligo-pectin modified by the flavonoid compound can better adsorb active ingredients in plants, further improve the activity of acetaldehyde dehydrogenase, accelerate alcohol metabolism in vivo and play a double role in dispelling effects of alcohol and protecting liver. The invention adopts the means of freezing and crushing, supercritical extraction, physical adsorption and the like to prepare the low-polymer pectin compound, and aims to provide a novel compound medicinal granule which is safe and efficient and can synergistically enhance the effects of relieving alcoholism and vomiting and protecting liver and spleen.

Disclosure of Invention

The invention aims to provide a preparation method of an oligo-pectin composite granule with an anti-alcoholism effect aiming at the defects of the prior art. The detailed technical scheme of the invention is as follows.

A preparation method of an oligo pectin composite electuary with an anti-alcoholism effect comprises the following steps:

(1) crushing: freezing and pulverizing radix Puerariae, fructus crataegi, Glycyrrhrizae radix, and pericarpium Citri Tangerinae to obtain powder with particle size of 30-200 μm;

(2) and (3) extraction: and (3) processing the crushed raw materials obtained in the step (1) by adopting a supercritical extraction technology to extract active ingredients in the materials.

(3) Adsorption: adding the extract obtained in the step (2) into an oligomerization modified pectin solution, stirring at 45-65 ℃ for 12-24h, and then freeze-drying the adsorption solution;

(4) and (3) granulation: and (4) uniformly mixing the oligo-pectin freeze-dried powder obtained in the step (3) with glutathione, rice bran fatty alkanol, potassium citrate and citric acid, adding a small amount of auxiliary materials, granulating, drying and crushing to obtain the composite electuary.

Further, in the step (1), the cold crushing temperature is-150 ℃ to-90 ℃.

Further, in the step (1), the addition amount of the kudzuvine root is 1-5 parts, the hawthorn fruit is 2-6 parts, the liquorice is 2-4 parts, and the dried orange peel is 2-5 parts.

Further, in the step (2), the raw material crushed by the supercritical extraction technology is 20-30% of the volume of the extraction kettle.

Further, in the step (2), the flow rate of carbon dioxide in the supercritical extraction process is 27-30 kg/h.

Further, in the step (2), the supercritical extraction time is 90-120 min.

Further, in the step (3), the molecular weight of the oligomeric modified pectin is 10-30 ku.

Further, in the step (3), the grafting ratio of the flavonoid compound in the oligomerization modified pectin is not less than 100 mg/g.

Further, in the step (4), the addition amount of the low pectin freeze-dried powder is 5-10 parts, 2-5 parts of glutathione, 2-4 parts of rice bran fatty alkanol, 1-3 parts of potassium citrate and 1-3 parts of citric acid.

Compared with the prior art, the invention has the following advantages and beneficial effects:

(1) compared with the existing formula of the anti-alcohol drug, the compound electuary provided by the invention is prepared from drugs or foods with homology of medicine and food according to a certain compatibility, has the effects of helping digestion and preventing body injury after drinking, is safe to take and is easy to accept.

(2) The invention adopts the freezing and crushing and supercritical extraction technology, greatly improves the yield and activity of the extracted substances, and avoids degradation and inactivation caused by high-temperature extraction of active substances.

Drawings

FIG. 1 is a process flow diagram of the present invention;

FIG. 2 is a histogram of the anti-hangover effect of the composite granules prepared in examples 1 to 4;

FIG. 3 is a bar graph of the activity of mouse liver tissue alcohol dehydrogenase of the complex granules prepared in examples 1 to 4;

FIG. 4 is a histogram of the activity of mouse liver tissue GSH prepared with the composite granules of examples 1-4;

FIG. 5 is a histogram of the activity of SOD in liver tissue of mice with the composite granules prepared in examples 1-4;

Detailed Description

The technical solutions of the present invention are further described in detail below with reference to specific examples and drawings, but the scope and implementation of the present invention are not limited thereto.

Example 1

Mixing 3 parts of radix Puerariae, 3 parts of fructus crataegi, 2 parts of Glycyrrhrizae radix, and 2 parts of pericarpium Citri Tangerinae, placing in a freezing pulverizer, and pulverizing at-120 deg.C to obtain particles of 50 μm; performing carbon dioxide supercritical extraction on the crushed raw materials, setting the flow of carbon dioxide to be 27kg/h, setting the crushed raw materials to be 25% of the volume of the extraction kettle, and extracting for 90 min; mixing the obtained extract with oligomeric modified pectin solution, stirring at 55 deg.C for 16 hr, freeze drying the obtained adsorption solution, and pulverizing; and (2) uniformly mixing 10 parts of freeze-dried powder, 5 parts of glutathione, 2 parts of rice bran fatty alkanol, 1 part of potassium citrate and 1 part of citric acid, adding a small amount of auxiliary materials, granulating, drying and crushing to obtain the composite electuary.

Example 2

Mixing 2 parts of radix puerariae, 4 parts of hawthorn, 2 parts of liquorice and 2 parts of dried orange peel, and placing the mixture in a freezing pulverizer, wherein the pulverizing temperature is set to-140 ℃, and the pulverizing particle size is 40 mu m; performing carbon dioxide supercritical extraction on the crushed raw materials, setting the flow of carbon dioxide to be 28kg/h, setting the crushed raw materials to be 25% of the volume of the extraction kettle, and extracting for 120 min; mixing the obtained extract with oligomeric modified pectin solution, stirring at 55 deg.C for 20 hr, freeze drying the obtained adsorption solution, and pulverizing; and (3) uniformly mixing 10 parts of freeze-dried powder, 4 parts of glutathione, 2 parts of rice bran fatty alkanol, 1 part of potassium citrate and 2 parts of citric acid, adding a small amount of auxiliary materials, granulating, drying and crushing to obtain the composite electuary.

Example 3

Mixing 4 parts of radix puerariae, 2 parts of hawthorn, 1 part of liquorice and 3 parts of dried orange peel, and placing the mixture in a freezing pulverizer, wherein the pulverizing temperature is set to-150 ℃, and the pulverizing particle size is 60 mu m; performing carbon dioxide supercritical extraction on the crushed raw materials, setting the flow of carbon dioxide to be 27kg/h, setting the crushed raw materials to be 29 percent of the volume of the extraction kettle, and extracting for 120 min; mixing the obtained extract with oligomeric modified pectin solution, stirring at 60 deg.C for 20 hr, freeze drying the obtained adsorption solution, and pulverizing; and uniformly mixing 8 parts of freeze-dried powder, 4 parts of glutathione, 1 part of rice bran fatty alkanol, 1 part of potassium citrate and 1 part of citric acid, adding a small amount of auxiliary materials, granulating, drying and crushing to obtain the composite electuary.

Example 4

Mixing 5 parts of radix Puerariae, 4 parts of fructus crataegi, 3 parts of Glycyrrhrizae radix, and 3 parts of pericarpium Citri Tangerinae, placing in a freezing pulverizer, and pulverizing at-100 deg.C to obtain powder with particle size of 80 μm; performing carbon dioxide supercritical extraction on the crushed raw materials, setting the flow of carbon dioxide to be 30kg/h, setting the crushed raw materials to be 25% of the volume of the extraction kettle, and extracting for 120 min; mixing the obtained extract with oligomeric modified pectin solution, stirring at 55 deg.C for 16 hr, freeze drying the obtained adsorption solution, and pulverizing; and uniformly mixing 8 parts of freeze-dried powder, 6 parts of glutathione, 3 parts of rice bran fatty alkanol, 1 part of potassium citrate and 1 part of citric acid, adding a small amount of auxiliary materials, granulating, drying and crushing to obtain the composite electuary.

Functional assay experiment

The composite granules prepared in the embodiments 1 to 4 are measured for the capability of alleviating hangover, the activity of tissue alcohol dehydrogenase, the content of GSH and the activity of SOD as follows:

an anti-alcohol experiment:

100 mice were taken, half female and half male, and fasted overnight (12h) before the experiment. Each group was administered with 52% (v/v) Chinese liquor at a dose of 0.25mL/10g, and a group of blanks was administered with gastric lavage saline. After the model group and the administration group are drunk for 30min, the normal saline with the concentration of 0.25mL/10g and the compound granules with the concentration of 1-4 in the embodiment are respectively used for drenching, and the hangover alleviating rate of the mice after 1h is observed. After the experiment, the liver tissues of the mice are collected, and the contents of ADH, GSH and SOD are measured according to the kit instructions.

The histogram of the antialcoholism ability of the composite granules prepared in the embodiments 1 to 4 is shown in fig. 2, and the composite granules have remarkable antialcoholism ability; in addition, the Acetaldehyde Dehydrogenase (ADH), the GSH content and the SOD activity of the mice are obviously improved in each embodiment. The pectin oligomer composite granules can accelerate the conversion of acetaldehyde into acetic acid and promote the decomposition of alcohol so as to achieve the effects of relieving alcoholism and protecting liver.

The above embodiments are preferred embodiments of the present invention, but the present invention is not limited to the above embodiments, and any other changes, modifications, substitutions, combinations, and simplifications which do not depart from the spirit and principle of the present invention should be construed as equivalents thereof, and all such changes, modifications, substitutions, combinations, and simplifications are intended to be included in the scope of the present invention.