阅读说明：本技术 一种改善贫血的组合物、其功能食品、制备方法及应用 (Composition for improving anemia, functional food thereof, preparation method and application ) 是由 贺砥砥 林波 田鑫 于 2021-08-09 设计创作，主要内容包括：本发明涉及一种改善贫血的组合物、其功能食品、制备方法及应用,所述的改善贫血的组合物包括如下重量份的原料：牛骨髓多肽粉5-25份、红枣粉10-30份、牛血蛋白多肽粉20-40份、枸杞粉12-25份、低聚果糖13-27份。本发明所述的改善贫血的组合物,从缺铁性贫血中铁的吸收利用率及造血功能的恢复等原因入手,通过多组分的相互配合,协同作用,可达到有效调节与改善贫血人群贫血状况的目的。对于饮食中缺乏铁、过分的节食或饮食不规律、消化道功能减弱、营养吸收差、再生障碍性贫血等造成的贫血有效率高、安全性好。(The invention relates to a composition for improving anemia, a functional food, a preparation method and application thereof, wherein the composition for improving anemia comprises the following raw materials in parts by weight: 5-25 parts of bovine bone marrow polypeptide powder, 10-30 parts of red date powder, 20-40 parts of bovine blood protein polypeptide powder, 12-25 parts of medlar powder and 13-27 parts of fructo-oligosaccharide. The composition for improving the anemia starts from the reasons of absorption and utilization rate of iron in the iron-deficiency anemia, recovery of hematopoietic function and the like, and can achieve the purpose of effectively regulating and improving the anemia condition of anemia people through mutual cooperation and synergistic action of multiple components. Has high effective rate and good safety for anemia caused by iron deficiency, excessive diet or irregular diet, weakened digestive tract function, poor nutrient absorption, aplastic anemia and the like.)

1. The composition for improving anemia is characterized by comprising the following raw materials in parts by weight:

5-25 parts of bovine bone marrow polypeptide powder, 10-30 parts of red date powder, 20-40 parts of bovine blood protein polypeptide powder, 12-25 parts of medlar powder and 13-27 parts of fructo-oligosaccharide.

2. The composition for improving anemia according to claim 1, comprising the following raw materials in parts by weight:

15 parts of bovine bone marrow polypeptide powder, 18 parts of red date powder, 30 parts of bovine blood protein polypeptide powder, 16 parts of medlar powder and 21 parts of fructo-oligosaccharide.

3. The composition for improving anemia according to claim 1 or 2, wherein said bovine bone marrow polypeptide powder is prepared by the following method: crushing and cleaning fresh ox stick bone, adding water, decocting, cooling to separate out oil, and filtering to remove oil to obtain filtrate; adding animal protease into the filtrate for enzymolysis, filtering, concentrating, adjusting pH, inactivating enzyme, filtering, concentrating, and drying.

4. The composition for improving anemia according to claim 3, wherein water is added according to a mass ratio of the bovine clavus to the water of 1:2-5 for boiling, the boiling temperature is 100 ℃, the boiling time is 3-5 hours, and then the temperature is reduced to below 75 ℃ to separate out oil; the enzymolysis is carried out by adding 200000-500000u/g animal protease, the enzymolysis temperature is 35-65 ℃, and the enzymolysis time is 6-7 h.

5. The composition for improving anemia according to claim 1 or 2, wherein the red date powder is instant red date powder, and the instant red date powder is prepared by the following method: cleaning fructus Jujubae, grinding, adding 14-16 times of water, extracting at 85-95 deg.C for 3-4 hr to obtain water extractive solution, filtering, concentrating, drying, and sieving.

6. The composition for improving anemia according to claim 1 or 2, wherein said bovine blood protein polypeptide powder is prepared by the following method: centrifuging bovine blood to remove plasma to obtain blood cells; adding deionized water into the blood cells, then carrying out ultrasonic treatment for assisting hemolysis, then carrying out refrigerated centrifugation, and collecting supernatant to obtain hemoglobin liquid; adding trypsin and neutral protease into hemoglobin solution for enzymolysis, filtering, concentrating, adjusting pH, inactivating enzyme, filtering, concentrating, and drying.

7. The composition for improving anemia as claimed in claim 6, wherein the enzymolysis is performed by adding 2000000-3500000u/g trypsin and neutral protease, the enzymolysis time is 5-7h, pH is 6-9, and temperature is 35-55 ℃.

8. The method for preparing a composition for improving anemia according to any one of claims 1 to 7, comprising the steps of: respectively taking bovine bone marrow polypeptide powder, red date powder, bovine blood protein polypeptide powder, medlar powder and fructo-oligosaccharide, and uniformly mixing the raw materials to obtain the feed.

9. A functional food for ameliorating anemia, comprising the anemia-ameliorating composition according to any one of claims 1 to 7.

10. Use of a composition according to any one of claims 1 to 7 for the preparation of a functional food for ameliorating anemia.

Technical Field

The invention belongs to the technical field of food, and particularly relates to a composition for improving anemia, a functional food thereof, a preparation method and an application.

Background

In recent years, patients with hyperlipidemia, obesity, and diabetes have been increasing because of the fact that "rich diseases" are frequently caused by overnutrition and unbalanced diet. At the same time, the incidence of anemia, often associated with "malnutrition", is high. Sampling survey shows that about one fifth of urban and rural residents in China have anemia symptoms of different degrees. Anemia results when the amount of hemoglobin and the number of red blood cells in the blood are below normal. Debilitation, dizziness, insomnia, tinnitus, palpitation, pale complexion, and the like are common symptoms of anemia. Anemia is caused by various reasons, and besides common nutrient deficiency, anemia may be caused by chronic blood loss, bone marrow hematopoietic disorder or hemolytic disease, and the most common nutritional deficiency anemia is. Nutritional anemia refers to anemia caused by deficiency of nutrients including iron, folic acid, vitamin B12, vitamin B6, vitamin A, vitamin C, protein, copper and the like, which are involved in the formation of hemoglobin and red blood cells due to malnutrition, wherein iron deficiency anemia caused by iron deficiency is the most common anemia. Iron deficiency causes depletion of stored iron in the body, and hemoglobin synthesis decreases, thereby affecting anemia caused by erythropoiesis. According to the report of the world health organization, the incidence rate of iron-deficiency anemia is higher than 50% in the main anemia population such as children and pregnant women. The elderly are also anemia-prone people. The organ function of the old people is declined to different degrees, and the old people are influenced by chronic diseases, comorbidities and multiple medicines, and the problems of malnutrition, anemia and the like are caused by insufficient intake of early-saturation food due to the reduction of living or activity capacity.

There are two main sources of iron in the diet: heme iron and non-heme iron. Heme iron is mainly obtained from meat foods, and is more easily absorbed and utilized in the human body than non-heme iron in grains and vegetables. At present, products aiming at improving the anemia condition of anemia people mostly focus on supplementing iron content, and neglect the absorption and utilization rate of iron and the recovery of hematopoietic function.

Disclosure of Invention

The invention aims to provide a composition for improving anemia, a functional food thereof, a preparation method and application. The composition has high absorption and utilization rate while supplementing various nutrient elements required by human hematopoiesis, and simultaneously promotes the recovery of hematopoiesis function, so that the composition can effectively regulate and improve anemia symptoms of anemia people, and has high effective rate and good safety on anemia caused by iron deficiency, excessive diet or irregular diet, weakened digestive tract function, poor nutrient absorption, aplastic anemia and the like.

In order to achieve the purpose, the invention adopts the following technical scheme:

the invention provides a composition for improving anemia, which comprises the following raw materials in parts by weight: 5-25 parts of bovine bone marrow polypeptide powder, 10-30 parts of red date powder, 20-40 parts of bovine blood protein polypeptide powder, 12-25 parts of medlar powder and 13-27 parts of fructo-oligosaccharide. Preferably, the feed comprises the following raw materials in parts by weight: 10-20 parts of bovine bone marrow polypeptide powder, 15-20 parts of red date powder, 25-35 parts of bovine blood protein polypeptide powder, 14-18 parts of medlar powder and 16-20 parts of fructo-oligosaccharide. More preferably, the feed comprises the following raw materials in parts by weight: 15 parts of bovine bone marrow polypeptide powder, 18 parts of red date powder, 30 parts of bovine blood protein polypeptide powder, 16 parts of medlar powder and 21 parts of fructo-oligosaccharide.

The bone marrow is the main hematopoietic organ and the origin of various blood cells, and the support, induction and regulation of the bone marrow hematopoietic microenvironment on the proliferation and differentiation of blood cells are realized by various growth factors in the matrix. The bovine bone marrow polypeptide powder contains a large amount of active ingredients such as cell factors, hormones, mineral substances (Ca, P, Fe, Zn, Cu and the like), bioactive polypeptides and the like, and can enhance the immunity of an organism and supplement trace elements required by the organism. Wherein a tetrapeptide molecule with the molecular weight of 487 exists in bovine bone marrow, and has the effects of promoting the granulo-monocytic progenitor cells to enter a DNA synthesis stage, stimulating the granulo-monocytic progenitor cells to proliferate, and promoting the recovery of hematopoietic function on a hematopoietic system. Meanwhile, the function of phagocyte and Natural Killer (NK) cell of human body is improved, and the function of T lymphocyte of human body is promoted to a certain extent.

Heme iron, also known as iron porphyrin, is a ferriporphyrin compound composed of porphyrin and a molecule of ferrous iron, and has an iron content of 1.0-2.5%, and a higher absorption rate than other iron enhancers. The animal blood is rich in heme iron. The blood of the cattle is rich in nutrition, and the content of various components in the blood is quite stable. Bovine blood consisted of 80.9% water, 17.3% protein, 0.5% fat, 0.5% carbohydrate and 0.8% minerals, with the exception of iron (36.3 mg/100 g blood), which was similar to the composition of meat, which is ten times as high as it is. The dry matter content of blood is about 19%, the protein content of dry matter is up to 90%, its nutritional properties are first reflected in the protein content, its amino acid composition is balanced, containing 18 amino acids, of which 8 essential amino acids and 2 semi-essential amino acids. Also contains appropriate amount of minerals such as calcium, phosphorus, ferrum, manganese, zinc, etc., and physiologically active substances such as thiamine, riboflavin, pantothenic acid, folic acid, vitamin B12, etc. The bioactive peptide with edible animal blood as protein source is prepared through converting globulin in blood into small molecular peptide via modern biotechnology. The small molecular blood peptide not only has good solubility, low viscosity and gel formation resistance, but also is fast to digest and absorb in vivo, and is suitable for being used as a main source of protein in the diet of patients who cannot digest and absorb complete protein, patients suffering from stomach diseases and patients suffering from liver diseases; is beneficial to the absorption and utilization of mineral elements; some small peptides also have immunopotentiating activity and the like. The heme iron and the small molecular weight peptide in the bovine blood protein polypeptide powder are easy to be absorbed by a human body, and meanwhile, the iron element and the amino acid required by the hematopoiesis of the human body can be quickly supplemented. Folic acid, in the form of a coenzyme, promotes purine and pyrimidine biosynthesis, i.e., is involved in DNA and RNA synthesis. Folic acid plays an important role in protein synthesis, cell division and growth, and has a promoting effect on the formation of normal red blood cells. Vitamin B12 improves folic acid utilization rate and promotes erythrocyte development and maturation. Methylmalonyl-coa is converted to succinyl-coa, which is involved in the tricarboxylic acid cycle, where succinyl-coa is involved in the synthesis of heme. It is also involved in the synthesis of deoxynucleic acid (DNA), the metabolism of fat, carbohydrate and protein, and the synthesis of nucleic acid and protein.

The red dates are sweet in taste and warm in nature, and can tonify spleen and stomach, tonify qi and promote fluid production, nourish heart and moisten lung, nourish blood and calm nerves. The red dates contain more than 50 chemical substances and 36 trace elements. Mainly comprises sugar, protein, amino acid, fat, polyphenol, cyclic adenosine monophosphate, cyclic guanosine monophosphate, triterpene and glycosides thereof, alkaloid, inorganic elements, vitamins and other active substances. The high vitamin content in the red dates has a health-care effect on human capillary vessels; the flavonoids have tranquilizing, hypnotic and blood pressure lowering effects. Cyclic adenosine monophosphate has the functions of regulating the metabolism of saccharides, lipids and proteins, promoting the synthesis and secretion of certain hormones and neurotransmitters in a body, participating in the proliferation and differentiation of cells and the like. The blood replenishing effect of red dates is the result of the comprehensive effect of various active ingredients such as polysaccharide, cyclic adenosine monophosphate, vitamins, inorganic salts and the like. The red jujube extract can obviously increase the serum iron content, iron saturation and the liver iron content of rats with iron deficiency anemia, and obviously reduce the serum unsaturated iron binding force, and the blood nourishing and hematogenesis functions of the red jujubes are proved from the modern pharmacology perspective.

The fructus Lycii is rich in fructus Lycii polysaccharide, fat, protein, free amino acids, taurine, betaine, vitamin B1, vitamin B2, vitamin E, and vitamin C, especially carotenoid. In addition, it also contains a large amount of mineral elements, such as potassium, sodium, calcium, magnesium, iron, copper, manganese, zinc, selenium, etc. The lycium barbarum polysaccharide can act on main immunocompetent cells such as T cells and B cells and the like, and can regulate the cellular immune function and the humoral immune function of an organism. The lycium barbarum polysaccharide can effectively enhance the phagocytic capacity of phagocytes, promote the proliferation of T lymphocytes, increase the content of serum IgG and enhance the activity of complement. The long-term consumption of the medlar is beneficial to improving the nonspecific immunity function and the specific immunity function of the organism. Carotenoids are converted into vitamin A in human body, and the vitamin A can reduce infectious anemia by regulating hematopoiesis and enhancing immunity to infectious diseases, regulate iron metabolism and the like to influence the occurrence of anemia.

The fructo-oligosaccharide is a water-soluble dietary fiber, can not be digested and absorbed by stomach and small intestine, but can form fructo-oligosaccharide-mineral complex with minerals, and after reaching large intestine, the fructo-oligosaccharide is fermented by bacteria to generate short chain fatty acid, and release mineral elements (Ca)²⁺、Mg²⁺、Fe²⁺And Zn²⁺Etc.), and the dissolution rate of minerals such as calcium, magnesium, iron, zinc and the like is increased under acidic conditions because the short-chain fatty acid reduces the pH value of the intestinal tract, and in addition, the short-chain fatty acid, particularly butyric acid, can stimulate the growth of conjunctival cells and improve the intestinal mucosa separationThe absorption capacity of the seed. The fructo-oligosaccharide can promote small intestine peristalsis, accelerate degradation and elimination of spoilage substances in intestinal tract, and has effects of loosening bowel to relieve constipation, improving stool properties, and preventing and relieving constipation. The fructo-oligosaccharide can rapidly proliferate Bacillus bifidus, which can synthesize vitamins B1, B2, B6, B12, nicotinic acid, folic acid, etc. in intestinal tract.

The composition provided by the invention has excellent effects of enriching blood, nourishing blood and generating blood through the synergistic effect of all the components, and can effectively regulate and improve the anemia condition of anemia people.

According to the composition for improving anemia, the bovine bone marrow polypeptide powder is prepared by the following method: crushing and cleaning fresh ox stick bone, adding water, decocting, cooling to separate out oil, and filtering to remove oil to obtain filtrate; adding animal protease into the filtrate for enzymolysis, filtering, concentrating, adjusting pH, inactivating enzyme, filtering, concentrating, and drying. Preferably, the animal protease is alkaline protease and trypsin in a mass ratio of 1:2-4, water is added into the animal protease and boiled according to a mass ratio of 1:2-5 between the ox clavus and the water, the boiling temperature is 100 ℃, the boiling time is 3-5 hours, and then the temperature is reduced to below 75 ℃ to separate out grease; the enzymolysis is carried out by adding 200000-500000u/g animal protease, the enzymolysis temperature is 35-65 ℃, and the enzymolysis time is 6-7 h. The bovine bone marrow polypeptide powder prepared by the method has the peptide content of 90-95 percent, the average molecular weight of the bovine bone marrow polypeptide is 300-500Da, and the hydroxyproline content is more than 9 percent. By evaluation of cytotoxicity experimental data, the bovine bone marrow polypeptide powder is safe and has no side effect.

According to the composition for improving anemia, the red date powder is instant red date powder, and the instant red date powder is prepared by the following method: cleaning fructus Jujubae, grinding, adding 14-16 times of water, extracting at 85-95 deg.C for 3-4 hr to obtain water extractive solution, filtering, concentrating, drying, and sieving.

According to the composition for improving anemia, the bovine blood protein polypeptide powder is prepared by the following method: centrifuging bovine blood to remove plasma to obtain blood cells; adding deionized water into the blood cells, then carrying out ultrasonic treatment for assisting hemolysis, then carrying out refrigerated centrifugation, and collecting supernatant to obtain hemoglobin liquid; adding trypsin and protease into hemoglobin solution for enzymolysis, filtering, concentrating, adjusting pH, inactivating enzyme, filtering, concentrating, and drying. Preferably, 2000000-3500000u/g trypsin and neutral protease are added for enzymolysis, the mass ratio of the trypsin to the neutral protease is 1-3:1, the enzymolysis time is 5-7h, the pH value is 6-9, and the temperature is 35-55 ℃. The bovine blood protein polypeptide powder prepared by the method has the peptide content of 70-80 percent, the average molecular weight of the bovine blood protein polypeptide is 400-600Da, and the iron content is more than 0.25 percent. By evaluation of cytotoxicity experimental data, the bovine blood protein polypeptide powder is safe and has no side effect.

The preparation method of the composition for improving the anemia has no special requirements in principle, and the raw materials are uniformly mixed.

It is understood that the composition for improving anemia of the present invention can be prepared into various forms of functional foods for improving anemia by adding conventional other auxiliary materials according to the conventional process in the art. The scope of protection of the present invention therefore also relates to functional food comprising the composition according to the invention and to the use of the composition according to the invention for preparing functional food for ameliorating anemia.

The invention has the beneficial effects that:

(1) the composition starts from the reasons of absorption and utilization rate of iron in iron-deficiency anemia, recovery of hematopoietic function and the like, and can achieve the purpose of effectively regulating and improving the anemia condition of anemia people through the mutual cooperation and synergistic effect of multiple components. On one hand, on the other hand, the iron heme in the bovine blood protein polypeptide powder, the iron in the bovine bone marrow polypeptide powder and the carotenoid in the Chinese wolfberry powder have a synergistic effect, so that the iron element required by human hematopoiesis is quickly supplemented, the bioavailability of the iron is increased, any discomfort symptom of the digestive tract cannot be generated, and the symptoms of fatigue, drowsiness, weakness and the like of anemia people can be quickly relieved. On the other hand, the bovine bone marrow polypeptide powder, the bovine blood protein polypeptide powder and the red date powder have a synergistic effect, and the small molecular peptides, the amino acids and the amino acids in the red date powder in the bovine blood protein polypeptide powder and the bovine bone marrow polypeptide powder provide sufficient raw materials for synthesis of hemoglobin and blood cells. Meanwhile, folic acid in the bovine blood protein peptide powder, vitamin B12, cyclic adenosine monophosphate in the red date powder and tetrapeptide in the bovine bone marrow polypeptide powder promote the myelomonocytic hematopoietic progenitor cells to enter a DNA synthesis period, stimulate the myelomonocytic progenitor cells to proliferate, promote the formation, development and maturation of the red blood cells, further restore the hematopoietic function, and effectively improve the symptoms of insomnia, dizziness, pale complexion and the like of anemic people. Tests prove that the effective rate of the composition for treating general symptoms of anemia (hypodynamia, dizziness, insomnia, tinnitus, palpitation and pale complexion) and other symptoms (polyuria at night, anorexia and cold hands and feet) is up to 86 percent, which shows that the composition has a remarkable effect of improving the anemia.

(2) The composition does not contain auxiliary materials such as cane sugar, maltodextrin, pigment, essence and the like, and is added with functional oligosaccharide, so that the composition has natural taste, is safe and reliable, and can be used as functional food for improving anemia symptoms corresponding to anemia people for long-term eating.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more apparent, the technical solutions of the present invention will be described in detail below. It is to be understood that the described embodiments are merely a few embodiments of the invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the examples given herein without any inventive step, are within the scope of the present invention.

Example 1

The embodiment provides a composition for improving anemia, which comprises the following raw materials in parts by weight:

5kg of bovine bone marrow polypeptide powder, 30kg of instant red date powder, 20kg of bovine blood protein polypeptide powder, 25kg of medlar powder and 20kg of fructo-oligosaccharide;

the bovine bone marrow polypeptide powder is prepared by the following method: fresh ox stick bones of agriculture and animal husbandry product enterprises which obtain Muslim certification in Mengdong area are taken, washed with cold water and warm water for 3 times, and stored in a freezer at the temperature of-20 ℃ after bone fragments and meat are removed; crushing fresh ox bone with a bone crusher, washing with cold water, putting into a tank, adding water 2 times the mass of the fresh ox bone, heating the temperature in the tank to 100 ℃, stirring and heating for 4 hours, reducing the temperature in the tank to 75 ℃ to separate out grease, and filtering the upper grease to obtain filtrate; adding 200000u/g animal protease (alkaline protease and trypsin at a mass ratio of 1: 2) into the filtrate, performing enzymolysis at 35 deg.C for 7h, filtering, concentrating, adjusting pH to 9.0, heating for 5min (80 deg.C), inactivating enzyme, filtering, concentrating, spray drying, and sieving with 80 mesh sieve to obtain bovine bone marrow polypeptide powder. Through detection, the peptide content of the obtained bovine bone marrow polypeptide powder is 90%, the average molecular weight of the bovine bone marrow polypeptide is 500Da, and the hydroxyproline content is 9%. By evaluating the cytotoxicity experimental data, the bovine bone marrow polypeptide powder is safe and has no side effect;

the instant red date powder is prepared by the following method: cleaning fructus Jujubae, grinding, adding 14 times of water, extracting at 85 deg.C for 4 hr to obtain water extractive solution, filtering, concentrating, drying, and sieving;

the bovine blood protein polypeptide powder is prepared by the following method: taking Muslim certified cattle from eastern region of inner Mongolia, collecting blood by humanized jugular vein, and centrifuging at high speed to remove blood plasma to obtain blood cells; adding blood cells into physiological saline with the same volume for washing and centrifuging twice, adding equal volume of deionized water, performing ultrasonic treatment for 20min under continuous stirring for assisting hemolysis, performing refrigerated centrifugation for 10min at 4 ℃, and collecting supernatant to obtain hemoglobin solution; adding 2000000u/g trypsin and neutral protease (mass ratio of trypsin to neutral protease is 1: 1) into hemoglobin solution, performing enzymolysis (time is 5h, pH value is 6, and temperature is 35 ℃), filtering, concentrating, adjusting pH value of concentrated solution to 9.0, heating for 10min (70 ℃), inactivating enzyme, filtering, concentrating, spray drying, and sieving with 80 mesh sieve to obtain bovine hemoglobin polypeptide powder. Through detection, the content of peptide in the obtained bovine blood protein polypeptide powder is 70%, the average molecular weight of the bovine blood protein polypeptide is 600Da, and the iron content is 0.25%. By evaluation of cytotoxicity experimental data, the bovine blood protein polypeptide powder is safe and has no side effect.

The preparation method of the composition for improving anemia described in this embodiment is: respectively taking bovine bone marrow polypeptide powder, instant red date powder, bovine blood protein polypeptide powder, medlar powder and fructo-oligosaccharide, uniformly mixing the raw materials, and packaging into 2.5 g/bag to obtain the composition for improving anemia.

Example 2

The embodiment provides a composition for improving anemia, which comprises the following raw materials in parts by weight:

10kg of bovine bone marrow polypeptide powder, 20kg of instant red date powder, 25kg of bovine blood protein polypeptide powder, 18kg of Chinese wolfberry powder and 27kg of fructo-oligosaccharide;

the bovine bone marrow polypeptide powder is prepared by the following method: taking fresh ox stick bones of agriculture and animal husbandry product enterprises which obtain Muslim certification in Mengdong region, washing with cold water and warm water for 5 times, removing bone fragments and meat, and storing in a freezer at-20 ℃; crushing fresh ox bone with a bone crusher, washing with cold water, putting into a tank, adding water 5 times the mass of the fresh ox bone, heating the temperature in the tank to 100 ℃, stirring and heating for 3 hours, reducing the temperature in the tank to 75 ℃, separating out grease, and filtering the upper grease to obtain filtrate; adding 500000u/g animal protease (alkaline protease and trypsin in a mass ratio of 1: 4 and at 65 ℃ for enzymolysis for 6 hours, filtering, concentrating, adjusting the pH value of a concentrated solution to 10.0, heating for 10min (60 ℃), inactivating enzyme, filtering, concentrating, spray drying, and sieving with a 100-mesh sieve to obtain bovine bone marrow polypeptide powder, wherein the obtained bovine bone marrow polypeptide powder has the peptide content of 95%, the average molecular weight of bovine bone marrow polypeptide is 300Da, and the content of hydroxyproline is 9.3%, and the bovine bone marrow polypeptide powder is safe and has no side effect through evaluation of cytotoxicity experimental data;

the instant red date powder is prepared by the following method: cleaning fructus Jujubae, grinding, adding 16 times of water, extracting at 95 deg.C for 3 hr to obtain water extractive solution, filtering, concentrating, drying, and sieving;

the bovine blood protein polypeptide powder is prepared by the following method: taking Muslim certified cattle from eastern region of inner Mongolia, collecting blood by humanized jugular vein, and centrifuging at high speed to remove blood plasma to obtain blood cells; adding blood cells into physiological saline with the same volume for washing and centrifuging twice, adding equal volume of deionized water, performing ultrasonic treatment for 20min under continuous stirring for assisting hemolysis, performing refrigerated centrifugation for 10min at 4 ℃, and collecting supernatant to obtain hemoglobin solution; adding 3500000u/g trypsin and protease (the mass ratio of the trypsin to the neutral protease is 3:1) into the hemoglobin liquid for enzymolysis (time is 7h, pH value is 9, and temperature is 55 ℃), filtering, concentrating, adjusting the pH value of the concentrated solution to 9.6, heating for 20min (60 ℃), inactivating enzyme, filtering, concentrating, spray drying, and sieving with a 100-mesh sieve to obtain the bovine hemoglobin polypeptide powder. Through detection, the content of peptides in the obtained bovine blood protein polypeptide powder is 80%, the average molecular weight of the bovine blood protein polypeptide is 400Da, and the iron content is 0.29%. By evaluation of cytotoxicity experimental data, the bovine blood protein polypeptide powder is safe and has no side effect.

The preparation method of the composition for improving anemia described in this embodiment is: respectively taking bovine bone marrow polypeptide powder, instant red date powder, bovine blood protein polypeptide powder, medlar powder and fructo-oligosaccharide, uniformly mixing the raw materials, and packaging into 2.5 g/bag to obtain the composition for improving anemia.

Example 3

The embodiment provides a composition for improving anemia, which comprises the following raw materials in parts by weight:

15kg of bovine bone marrow polypeptide powder, 18kg of instant red date powder, 30kg of bovine blood protein polypeptide powder, 16kg of Chinese wolfberry powder and 21kg of fructo-oligosaccharide;

the bovine bone marrow polypeptide powder is prepared by the following method: fresh ox stick bones of agriculture and animal husbandry product enterprises which obtain Muslim certification in Mengdong area are taken, washed with cold water and warm water for 3 times, and stored in a freezer at the temperature of-20 ℃ after bone fragments and meat are removed; crushing fresh ox bone with a bone crusher, washing with cold water, putting into a tank, adding water with the mass 3 times that of the fresh ox bone, heating the temperature in the tank to 100 ℃, stirring and heating for 5 hours, reducing the temperature in the tank to 75 ℃, separating out grease, and filtering the upper grease to obtain filtrate; adding 400000u/g animal protease (alkaline protease, trypsin, mass ratio of 1: 3) into the filtrate, performing enzymolysis at 50 deg.C for 6 hr, filtering, concentrating, adjusting pH to 9.8, heating for 10min (60 deg.C), inactivating enzyme, filtering, concentrating, spray drying, and sieving with 100 mesh sieve to obtain bovine bone marrow polypeptide powder. Through detection, the obtained bovine bone marrow polypeptide powder has the peptide content of 92%, the average molecular weight of the bovine bone marrow polypeptide is 350Da, and the hydroxyproline content is 10.2%. By evaluating the cytotoxicity experimental data, the bovine bone marrow polypeptide powder is safe and has no side effect;

the instant red date powder is prepared by the following method: cleaning fructus Jujubae, grinding, adding 15 times of water, extracting at 90 deg.C for 3.5 hr to obtain water extractive solution, filtering, concentrating, drying, and sieving to obtain;

the bovine blood protein polypeptide powder is prepared by the following method: taking Muslim certified cattle from eastern region of inner Mongolia, collecting blood by humanized jugular vein, and centrifuging at high speed to remove blood plasma to obtain blood cells; adding blood cells into physiological saline with the same volume for washing and centrifuging twice, adding equal volume of deionized water, performing ultrasonic treatment for 20min under continuous stirring for assisting hemolysis, performing refrigerated centrifugation for 10min at 4 ℃, and collecting supernatant to obtain hemoglobin solution; adding 3000000u/g trypsin and protease (the mass ratio of the trypsin to the neutral protease is 2:1) into the hemoglobin liquid for enzymolysis (the time is 6 hours, the pH value is 7.2, and the temperature is 50 ℃), filtering, concentrating, adjusting the pH value of the concentrated solution to 9.4, heating for 20min (60 ℃), inactivating the enzyme, filtering, concentrating, spray drying, and sieving with a 100-mesh sieve to obtain the bovine hemoglobin polypeptide powder. Through detection, the content of peptides in the obtained bovine blood protein polypeptide powder is 74%, the average molecular weight of the bovine blood protein polypeptide is 500Da, and the iron content is 0.33%. By evaluation of cytotoxicity experimental data, the bovine blood protein polypeptide powder is safe and has no side effect.

The preparation method of the composition for improving anemia described in this embodiment is: respectively taking bovine bone marrow polypeptide powder, instant red date powder, bovine blood protein polypeptide powder, medlar powder and fructo-oligosaccharide, uniformly mixing the raw materials, and packaging into 2.5 g/bag to obtain the composition for improving anemia.

Example 4

The embodiment provides a composition for improving anemia, which comprises the following raw materials in parts by weight:

20kg of bovine bone marrow polypeptide powder, 15kg of instant red date powder, 35kg of bovine blood protein polypeptide powder, 14kg of Chinese wolfberry powder and 16kg of fructo-oligosaccharide;

the rest is the same as example 1.

Example 5

The embodiment provides a composition for improving anemia, which comprises the following raw materials in parts by weight:

25kg of bovine bone marrow polypeptide powder, 10kg of instant red date powder, 40kg of bovine blood protein polypeptide powder, 12kg of Chinese wolfberry powder and 13kg of fructo-oligosaccharide;

the rest is the same as example 1.

Comparative example 1

The comparative example provides a composition for improving anemia, which is different from example 3 only in the composition of raw materials, and specifically, the composition comprises the following raw materials in parts by weight:

15kg of bovine bone marrow polypeptide powder, 30kg of bovine blood protein polypeptide powder, 16kg of medlar powder and 39kg of fructo-oligosaccharide.

Comparative example 2

The comparative example provides a composition for improving anemia, which is different from example 3 only in the composition of raw materials, and specifically, the composition comprises the following raw materials in parts by weight:

18kg of instant red date powder, 30kg of bovine hemoglobin polypeptide powder, 16kg of medlar powder and 36kg of fructo-oligosaccharide.

Test examples

At least one of general symptoms of anemia (hypodynamia, dizziness, insomnia, tinnitus, palpitation and pale complexion) and other symptoms (nocturia, anorexia and cold hands and feet) is 350 people in total, the age is 30-75 years old, 160 men and 190 women are randomly divided into 7 groups of 50 people, and the groups are respectively the groups of examples 1-5 and the groups of comparative examples 1-2. The composition of the corresponding group is eaten by each group respectively, and the eating method comprises the following steps: the composition is taken with warm water 2 times a day, 1 bag each time, and continuously taken for 3 months, and the examination records and the statistical results of all groups of volunteers are shown in Table 1.

The evaluation standard of the curative effect is as follows:

the effect is shown: the traditional Chinese medicine composition has more than 1 kind of main clinical symptoms of 9 kinds of symptoms of hypodynamia, dizziness, insomnia, tinnitus, palpitation, pale complexion, polyuria at night, anorexia and cold hands and feet, and has no other adverse reactions.

And (4) invalidation: 9 main clinical symptoms of hypodynamia, dizziness, insomnia, tinnitus, palpitation, pale complexion, nocturnal polyuria, anorexia and cold hands and feet are not improved or have adverse reactions.

The effective rate is the percentage of the total number of people in each group.

TABLE 1 test results

The test results of the compositions in examples 1-5 show that the effective rates are 66%, 78%, 86%, 72% and 70%, respectively, which shows that the composition has obvious improvement effect on the anemia symptom population;

the test results of the example 3 group, the comparative example 1 group and the comparative example 2 group show that the effective rate of the example 3 group is improved by 10 percent compared with the comparative example 1 group, and the effective rate of the example 3 group is improved by 14 percent compared with the comparative example 2 group, so that the effect is obvious. The improvement of the cold hands and feet is particularly prominent, and accounts for 55.8 percent of the effective number, and the improvement of the insomnia accounts for 37.2 percent of the effective number. Compared with a composition consisting of 4 raw materials of bovine bone marrow polypeptide powder, bovine blood protein polypeptide powder, medlar powder and fructo-oligosaccharide and a composition consisting of 4 raw materials of instant red date powder, bovine blood protein polypeptide powder, medlar powder and fructo-oligosaccharide, the composition consisting of 5 raw materials of bovine bone marrow polypeptide powder, instant red date powder, bovine blood protein polypeptide powder, medlar powder and fructo-oligosaccharide has an obviously better anemia improving effect, and the fact shows that the 5 raw materials of the composition provided by the invention can synergistically improve the anemia improving effect through interaction.

Typical case

Guo in 65 years old, cold hands and feet and dizziness of women, especially in winter, 1 bag of the composition for improving anemia, which is obtained in the embodiment 3 of the invention, is taken in the morning and evening every day, and after 7 days of continuous taking, the hands and the feet are hot and not numb. After eating for 30 days, people feel that the blood circulation is accelerated, the head is not dizzy and not burnt, and the whole people are spiritual.

For a senior male, the age of 70 years, dizziness and 3-4 times of night, 1 bag of each composition for improving anemia, which is obtained in the embodiment 3 of the invention, is taken in the morning and evening for 10 days, the head is not dizzy, the times of night are reduced, and the frequency of urination is improved. After the medicine is taken for 75 days, the patient does not get up at night, and the nocturia is obviously improved.

When a user takes 1 bag of composition for improving anemia, which is obtained in the embodiment 2 of the invention, in the morning and evening each day, the user feels sleepy in the noon after continuously taking the composition for improving anemia for 15 days, and the sleeping time in the evening is increased by 2 hours. After 45 days of taking, the face is ruddy, the symptoms of hypodynamia are obviously improved, and people are also spiritual.

Old women, age 40, irregular diet, poor digestive function, pale face, 1 bag each in the morning and evening, 18 days of continuous administration, ruddy complexion, spirits, appetite enhancement, and constipation improvement by taking the composition for improving anemia obtained in example 5 of the present invention.

Wangzhi, male, age 75, dizziness and headache, and by taking the composition for improving anemia, which is obtained in the embodiment 1 of the invention, 1 bag each in the morning and evening every day, and 15 days, symptoms of dizziness and headache are obviously improved.

The above description is only for the specific embodiments of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art can easily conceive of the changes or substitutions within the technical scope of the present invention, and all the changes or substitutions should be covered within the scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the appended claims.