阅读说明：本技术 一种能够脱除玉米赤霉烯酮的复合脱毒剂 (Composite detoxifying agent capable of removing zearalenone ) 是由 陈超 汪志强 张巍 陈国顺 傅建彬 何伟峰 于 2021-07-23 设计创作，主要内容包括：本发明公开了一种能够脱除玉米赤霉烯酮的复合脱毒剂,其原料按重量份包括：累托石30-45份、沸石8-19份、海泡石10-20份、稻壳炭5-17份、改性魔芋葡甘露聚糖5-13份、腐殖酸钠5-15份、维生素E 1-3份、复合菌2-11份、表面活性剂5-15份。本发明提出的能够脱除玉米赤霉烯酮的复合脱毒剂,其对玉米赤霉烯酮的脱毒率高,效果稳定。(The invention discloses a composite detoxicant capable of removing zearalenone, which comprises the following raw materials in parts by weight: 30-45 parts of rectorite, 8-19 parts of zeolite, 10-20 parts of sepiolite, 5-17 parts of rice hull carbon, 5-13 parts of modified konjac glucomannan, 5-15 parts of sodium humate, 1-3 parts of vitamin E, 2-11 parts of compound bacteria and 5-15 parts of surfactant. The composite detoxifying agent capable of removing zearalenone provided by the invention has high detoxifying rate and stable effect on zearalenone.)

1. A composite detoxifying agent capable of removing zearalenone is characterized by comprising the following raw materials in parts by weight: 30-45 parts of rectorite, 8-19 parts of zeolite, 10-20 parts of sepiolite, 5-17 parts of rice hull carbon, 5-13 parts of modified konjac glucomannan, 5-15 parts of sodium humate, 1-3 parts of vitamin E, 2-11 parts of compound bacteria and 5-15 parts of surfactant.

2. The composite detoxicant according to claim 1, wherein the rice husk charcoal is modified rice husk charcoal obtained by adding aqueous solution of octadecyl dimethyl benzyl ammonium chloride, rice husk charcoal and sodium acetate into a planetary ball mill, ball milling, drying and grinding.

3. The composite detoxicant according to claim 2, wherein the weight ratio of said rice husk charcoal, sodium acetate and octadecyl dimethyl benzyl ammonium chloride is 100: 2-5: 2-5.

4. The composite detoxicant according to claim 1, wherein said modified konjac glucomannan is graphene-modified konjac glucomannan; preferably, the preparation process of the graphene modified konjac glucomannan comprises the following steps: adding graphene oxide into water for dispersion, then adding sodium hydroxide and sodium chloroacetate, performing ultrasonic treatment at 35-60 ℃ for 3-5h, centrifuging, washing with hydrochloric acid, washing with water, and drying to obtain carboxylated graphene oxide; mixing carboxylated graphene oxide, dimethylformamide and thionyl chloride, reacting for 10-20h at 75-80 ℃, and obtaining acyl chlorinated graphene oxide after the reaction is finished; uniformly mixing acyl chloride graphene oxide, undecylenol, swollen konjac glucomannan, triethylamine, toluene and dimethylacetamide, stirring and reacting for 50-110h at 50-80 ℃, performing solid-liquid separation, washing and drying after the reaction is finished, adding the obtained product into water, adding 1-hexadecyl-3-methylimidazole bromide and didodecyldimethylammonium bromide, performing ultrasonic dispersion, and stirring for 10-20h at 55-65 ℃ to obtain the modified konjac glucomannan.

5. The composite detoxifying agent capable of removing zearalenone according to claim 4, wherein the weight ratio of graphene oxide, sodium hydroxide and sodium chloroacetate is 30-45: 2.4-4: 3-5; the weight volume ratio of the carboxylated graphene oxide to the dimethyl formamide to the thionyl chloride is 50-80 g: 2-30 ml: 20-40 ml; the weight ratio of the acyl chloride graphene oxide to the undecylenol to the konjac glucomannan to the triethylamine to the toluene to the dimethylacetamide is 1: 0.5-2: 5-15: 0.5-2: 5-15: 5-10; the total weight of the 1-hexadecyl-3-methylimidazole bromide and the didodecyldimethylammonium bromide is 0.05-0.2 times of the weight of the product, and the weight ratio of the 1-hexadecyl-3-methylimidazole bromide to the didodecyldimethylammonium bromide is 1: 3-6.

6. The composite detoxifying agent capable of removing zearalenone according to claim 1, wherein the composite bacteria is a mixture of a plurality of bacillus subtilis, clostridium tyrobutyricum, pediococcus pentosaceus and candida utilis.

7. The composite detoxifying agent capable of removing zearalenone according to claim 1, wherein the composite bacteria is a mixture of bacillus subtilis, clostridium tyrobutyricum, pediococcus pentosaceus and candida utilis, and the weight ratio of bacillus subtilis, clostridium tyrobutyricum, pediococcus pentosaceus and candida utilis is 3-10: 2-9: 1-5: 2-7.

8. The composite detoxicant according to any one of claims 1 to 7, wherein said surfactant is dioctadecyldimethylammonium chloride, cocamidopropyl betaine, polyethylene glycol 400 in a weight ratio of 3-10: 2-8: 1-4.

Technical Field

The invention relates to the technical field of detoxication agents, in particular to a composite detoxication agent capable of removing zearalenone.

Background

Zearalenone is an estrogen-like mycotoxin widely present in various food crops. Consumption of zearalenone containing foods by pregnant animals (including humans) can cause abortion, stillbirth and teratogenesis. The food made from wheat flour containing gibberellic disease can also cause poisoning symptoms of central nervous system, such as nausea, chill, headache, mental depression and ataxia. The removal of zearalenone has been a research hotspot. The adsorption method is the most effective detoxification method at present, the method is the most mature method in actual industrial production at present, various adsorbents are disclosed in the prior art, but the defect of poor adsorption effect still exists when the adsorption method is used for removing zearalenone, and the application of the adsorption method is limited.

Disclosure of Invention

Based on the technical problems in the background art, the invention provides a composite detoxicant capable of removing zearalenone, which has high detoxicant rate on zearalenone and stable effect.

The invention provides a composite detoxicant capable of removing zearalenone, which comprises the following raw materials in parts by weight: 30-45 parts of rectorite, 8-19 parts of zeolite, 10-20 parts of sepiolite, 5-17 parts of rice hull carbon, 5-13 parts of modified konjac glucomannan, 5-15 parts of sodium humate, 1-3 parts of vitamin E, 2-11 parts of compound bacteria and 5-15 parts of surfactant.

Preferably, the rice husk carbon is modified rice husk carbon obtained by adding an octadecyl dimethyl benzyl ammonium chloride aqueous solution, rice husk carbon and sodium acetate into a planetary ball mill, performing ball milling, drying and grinding.

Preferably, the weight ratio of the rice husk carbon, the sodium acetate and the octadecyl dimethyl benzyl ammonium chloride is 100: 2-5: 2-5.

Preferably, the modified konjac glucomannan is graphene-modified konjac glucomannan; preferably, the preparation process of the graphene modified konjac glucomannan comprises the following steps: adding graphene oxide into water for dispersion, then adding sodium hydroxide and sodium chloroacetate, performing ultrasonic treatment at 35-60 ℃ for 3-5h, centrifuging, washing with hydrochloric acid, washing with water, and drying to obtain carboxylated graphene oxide; mixing carboxylated graphene oxide, dimethylformamide and thionyl chloride, reacting for 10-20h at 75-80 ℃, and obtaining acyl chlorinated graphene oxide after the reaction is finished; uniformly mixing acyl chloride graphene oxide, undecylenol, swollen konjac glucomannan, triethylamine, toluene and dimethylacetamide, stirring and reacting for 50-110h at 50-80 ℃, performing solid-liquid separation, washing and drying after the reaction is finished, adding the obtained product into water, adding 1-hexadecyl-3-methylimidazole bromide and didodecyldimethylammonium bromide, performing ultrasonic dispersion, and stirring for 10-20h at 55-65 ℃ to obtain the modified konjac glucomannan.

Preferably, the weight ratio of the graphene oxide to the sodium hydroxide to the sodium chloroacetate is 30-45: 2.4-4: 3-5; the weight volume ratio of the carboxylated graphene oxide to the dimethyl formamide to the thionyl chloride is 50-80 g: 2-30 ml: 20-40 ml; the weight ratio of the acyl chloride graphene oxide to the undecylenol to the konjac glucomannan to the triethylamine to the toluene to the dimethylacetamide is 1: 0.5-2: 5-15: 0.5-2: 5-15: 5-10; the total weight of the 1-hexadecyl-3-methylimidazole bromide and the didodecyldimethylammonium bromide is 0.05-0.2 times of the weight of the product, and the weight ratio of the 1-hexadecyl-3-methylimidazole bromide to the didodecyldimethylammonium bromide is 1: 3-6.

Preferably, the compound bacteria are a mixture of multiple bacteria of bacillus subtilis, clostridium butyricum, pediococcus pentosaceus and candida utilis.

Preferably, the compound bacteria are a mixture of bacillus subtilis, clostridium tyrobutyricum, pediococcus pentosaceus and candida utilis, and the weight ratio of the bacillus subtilis to the clostridium tyrobutyricum to the pediococcus pentosaceus to the candida utilis is 3-10: 2-9: 1-5: 2-7.

Preferably, the surfactant is dioctadecyl dimethyl ammonium chloride, cocamidopropyl betaine and polyethylene glycol 400 in a weight ratio of 3-10: 2-8: 1-4.

Preferably, the composite detoxifying agent capable of removing zearalenone according to the present invention can be prepared according to the following process: uniformly mixing rectorite, zeolite and sepiolite, modifying by taking a surfactant as a modifier, and uniformly mixing with the rest raw materials to obtain the composite detoxicant capable of removing zearalenone.

Preferably, the rice husk charcoal of the invention is biochar obtained by taking rice husks of rice as raw materials and carbonizing the raw materials through a pyrolysis method.

Rectorite, zeolite, sepiolite and rice husk carbon all have porous structures, attract toxins through the action of electric charges and the like, and are adsorbed in the porous structures to achieve the effect of detoxification, and konjac glucomannan is a high-molecular polysaccharide and can generate adsorption force on the toxins through ionic bonds, hydrogen bonds and hydrophobic action; in the preparation process of the modified konjac glucomannan, firstly, the graphene oxide is carboxylated, then thionyl chloride is used as a raw material for acyl chlorination to obtain acyl chlorinated graphene oxide, then the acyl chlorinated graphene oxide, undecylene and swollen konjac glucomannan are used as raw materials, reaction conditions are controlled, the three are acted, and the obtained product is modified by using 1-hexadecyl-3-methylimidazole bromide and didodecyldimethylammonium bromide as modifiers to obtain the graphene modified konjac glucomannan, wherein the surface of the graphene modified konjac glucomannan is greatly enriched, the surface area of the graphene modified konjac glucomannan is larger, the graphene modified konjac glucomannan has higher affinity to zearalenone, the adsorption effect on zearalenone is better, and the defect of small adsorption amount of a simple inorganic adsorbent can be overcome; the composite bacteria can degrade zearalenone by adding the composite bacteria into the system, so that the zearalenone is inactivated, and the content of the zearalenone is reduced; the sodium humate contains a large number of structural rings, and active groups such as hydroxyl, quinonyl, methoxyl and the like on the rings have excellent adsorption effect on the zearalenone; the composite detoxifying agent capable of removing zearalenone comprises the raw materials, wherein rectorite is specifically used as a main material, zeolite, sepiolite, rice hull carbon, modified konjac glucomannan, sodium humate, vitamin E, composite bacteria and a surfactant are simultaneously matched, the content of each raw material is controlled, so that substances in different action directions are matched to play a synergistic effect, the detoxifying effect on zearalenone is as high as more than 96.7%, and the desorption rate is lower than 3.21%.

Detailed Description

The technical solution of the present invention will be described in detail below with reference to specific examples.

Example 1

The invention provides a composite detoxicant capable of removing zearalenone, which comprises the following raw materials in parts by weight: 38 parts of rectorite, 11 parts of zeolite, 15 parts of sepiolite, 11 parts of rice husk carbon, 10 parts of modified konjac glucomannan, 8 parts of sodium humate, 2 parts of vitamin E, 7 parts of composite bacteria and 11 parts of surfactant.

Example 2

The invention provides a composite detoxicant capable of removing zearalenone, which comprises the following raw materials in parts by weight: 45 parts of rectorite, 8 parts of zeolite, 20 parts of sepiolite, 5 parts of rice husk carbon, 7 parts of modified konjac glucomannan, 11 parts of sodium humate, 3 parts of vitamin E, 1 part of bacillus subtilis, 1 part of clostridium butyricum, 10 parts of dioctadecyl dimethyl ammonium chloride, 2 parts of cocamidopropyl betaine and 3 parts of polyethylene glycol 4003;

the rice husk carbon is modified rice husk carbon obtained by adding octadecyl dimethyl benzyl ammonium chloride aqueous solution, rice husk carbon and sodium acetate into a planetary ball mill, ball-milling, drying and grinding; the weight ratio of the rice husk carbon to the sodium acetate to the octadecyl dimethyl benzyl ammonium chloride is 100: 2: 5;

the modified konjac glucomannan is graphene-modified konjac glucomannan; the preparation process of the graphene modified konjac glucomannan comprises the following steps: adding graphene oxide into water for dispersion, then adding sodium hydroxide and sodium chloroacetate, performing ultrasonic treatment at 35 ℃ for 5 hours, centrifuging, washing with hydrochloric acid, washing with water, and drying to obtain carboxylated graphene oxide; mixing carboxylated graphene oxide, dimethylformamide and thionyl chloride, reacting for 20 hours at 75 ℃, and obtaining acylchlorinated graphene oxide after the reaction is finished; uniformly mixing acyl chloride graphene oxide, undecylenol, swollen konjac glucomannan, triethylamine, toluene and dimethylacetamide, stirring and reacting at 50 ℃ for 110 hours, carrying out solid-liquid separation, washing and drying after the reaction is finished, adding the obtained product into water, adding 1-hexadecyl-3-methylimidazole bromide and didodecyldimethylammonium bromide, carrying out ultrasonic dispersion, and stirring at 55 ℃ for 15 hours to obtain the modified konjac glucomannan; wherein, in the preparation process, the weight ratio of the graphene oxide to the sodium hydroxide to the sodium chloroacetate is 37: 2.4: 5; the weight volume ratio of the carboxylated graphene oxide to the dimethyl formamide to the thionyl chloride is 50 g: 30 ml: 20ml of the solution; the weight ratio of the acylchlorinated graphene oxide to the undecenol to the unswollen konjac glucomannan to the triethylamine to the toluene to the dimethylacetamide is 1: 0.5: 8: 2: 11: 10; the total weight of the 1-hexadecyl-3-methylimidazole bromide and the didodecyldimethylammonium bromide is 0.05 times of the weight of the product, and the weight ratio of the 1-hexadecyl-3-methylimidazole bromide to the didodecyldimethylammonium bromide is 1: 6.

example 3

The invention provides a composite detoxicant capable of removing zearalenone, which comprises the following raw materials in parts by weight: 30 parts of rectorite, 19 parts of zeolite, 10 parts of sepiolite, 11 parts of rice husk carbon, 13 parts of modified konjac glucomannan, 5 parts of sodium humate, 1 part of vitamin E, 5 parts of pediococcus pentosaceus, 6 parts of candida utilis, 3 parts of dioctadecyl dimethyl ammonium chloride, 8 parts of cocamidopropyl betaine and 1 part of polyethylene glycol 4001;

the rice husk carbon is modified rice husk carbon obtained by adding an octadecyl dimethyl benzyl ammonium chloride aqueous solution, rice husk carbon and sodium acetate into a planetary ball mill, performing ball milling, drying and grinding, wherein the weight ratio of the rice husk carbon to the sodium acetate to the octadecyl dimethyl benzyl ammonium chloride is 100: 5: 2;

the modified konjac glucomannan is graphene-modified konjac glucomannan; the preparation process of the graphene modified konjac glucomannan comprises the following steps: adding graphene oxide into water for dispersion, then adding sodium hydroxide and sodium chloroacetate, performing ultrasonic treatment at 60 ℃ for 3 hours, centrifuging, washing with hydrochloric acid, washing with water, and drying to obtain carboxylated graphene oxide; mixing carboxylated graphene oxide, dimethylformamide and thionyl chloride, reacting for 10 hours at 80 ℃, and obtaining acylchlorinated graphene oxide after the reaction is finished; uniformly mixing acyl chloride graphene oxide, undecylenol, swollen konjac glucomannan, triethylamine, toluene and dimethylacetamide, stirring and reacting for 50 hours at 80 ℃, carrying out solid-liquid separation, washing and drying after the reaction is finished, adding the obtained product into water, adding 1-hexadecyl-3-methylimidazole bromide and didodecyldimethylammonium bromide, carrying out ultrasonic dispersion, and stirring for 10 hours at 65 ℃ to obtain the modified konjac glucomannan; in the preparation process, the weight ratio of the graphene oxide to the sodium hydroxide to the sodium chloroacetate is 45: 4: 3; the weight volume ratio of the carboxylated graphene oxide to the dimethyl formamide to the thionyl chloride is 80 g: 2 ml: 40 ml; the weight ratio of the acyl chloride graphene oxide to the undecylenol to the konjac glucomannan to the triethylamine to the toluene to the dimethylacetamide is 1: 2: 5: 1: 5: 5; the total weight of the 1-hexadecyl-3-methylimidazole bromide and the didodecyldimethylammonium bromide is 0.2 times of the weight of the product, and the weight ratio of the 1-hexadecyl-3-methylimidazole bromide to the didodecyldimethylammonium bromide is 1: 3.

example 4

The invention provides a composite detoxicant capable of removing zearalenone, which comprises the following raw materials in parts by weight: 45 parts of rectorite, 9 parts of zeolite, 18 parts of sepiolite, 17 parts of rice husk charcoal, 5 parts of modified konjac glucomannan, 15 parts of sodium humate, 1 part of vitamin E, 1.2 parts of bacillus subtilis, 0.8 part of clostridium butyricum, 0.4 part of pediococcus pentosaceus, 1.6 parts of candida utilis, 3 parts of dioctadecyl dimethyl ammonium chloride, 7 parts of cocamidopropyl betaine and 1 part of polyethylene glycol 4001;

the rice husk carbon is modified rice husk carbon obtained by adding an octadecyl dimethyl benzyl ammonium chloride aqueous solution, rice husk carbon and sodium acetate into a planetary ball mill, performing ball milling, drying and grinding, wherein the weight ratio of the rice husk carbon to the sodium acetate to the octadecyl dimethyl benzyl ammonium chloride is 100: 3: 4;

the modified konjac glucomannan is graphene-modified konjac glucomannan; the preparation process of the graphene modified konjac glucomannan comprises the following steps: adding graphene oxide into water for dispersion, then adding sodium hydroxide and sodium chloroacetate, performing ultrasonic treatment at 38 ℃ for 3.5 hours, centrifuging, washing with hydrochloric acid, washing with water, and drying to obtain carboxylated graphene oxide; mixing carboxylated graphene oxide, dimethylformamide and thionyl chloride, reacting at 77 ℃ for 18 hours to obtain acylchlorinated graphene oxide after the reaction is finished; uniformly mixing acyl chloride graphene oxide, undecylenol, swollen konjac glucomannan, triethylamine, toluene and dimethylacetamide, stirring and reacting for 90 hours at 55 ℃, carrying out solid-liquid separation, washing and drying after the reaction is finished, adding the obtained product into water, adding 1-hexadecyl-3-methylimidazole bromide and didodecyldimethylammonium bromide, carrying out ultrasonic dispersion, and stirring for 20 hours at 58 ℃ to obtain the modified konjac glucomannan; wherein the weight ratio of the graphene oxide to the sodium hydroxide to the sodium chloroacetate is 30: 3: 4; the weight volume ratio of the carboxylated graphene oxide to the dimethyl formamide to the thionyl chloride is 70 g: 5 ml: 28 ml; the weight ratio of the acyl chloride graphene oxide to the undecylenol to the konjac glucomannan to the triethylamine to the toluene to the dimethylacetamide is 1: 0.5: 15: 0.5: 15: 8; the total weight of the 1-hexadecyl-3-methylimidazole bromide and the didodecyldimethylammonium bromide is 0.1 time of the weight of the product, and the weight ratio of the 1-hexadecyl-3-methylimidazole bromide to the didodecyldimethylammonium bromide is 1: 5.

example 5

The invention provides a composite detoxicant capable of removing zearalenone, which comprises the following raw materials in parts by weight: 38 parts of rectorite, 16 parts of zeolite, 13 parts of sepiolite, 8 parts of rice husk carbon, 7 parts of modified konjac glucomannan, 8 parts of sodium humate, 2 parts of vitamin E, 4 parts of bacillus subtilis, 0.8 part of clostridium butyricum, 2 parts of pediococcus pentosaceus, 1.2 parts of candida utilis, 4 parts of dioctadecyl dimethyl ammonium chloride, 0.8 part of cocamidopropyl betaine and 4001.2 parts of polyethylene glycol;

the rice husk carbon is modified rice husk carbon obtained by adding an octadecyl dimethyl benzyl ammonium chloride aqueous solution, rice husk carbon and sodium acetate into a planetary ball mill, performing ball milling, drying and grinding, wherein the weight ratio of the rice husk carbon to the sodium acetate to the octadecyl dimethyl benzyl ammonium chloride is 100: 2: 3;

the modified konjac glucomannan is graphene-modified konjac glucomannan; the preparation process of the graphene modified konjac glucomannan comprises the following steps: adding graphene oxide into water for dispersion, then adding sodium hydroxide and sodium chloroacetate, performing ultrasonic treatment at 50 ℃ for 4 hours, centrifuging, washing with hydrochloric acid, washing with water, and drying to obtain carboxylated graphene oxide; mixing carboxylated graphene oxide, dimethylformamide and thionyl chloride, reacting for 13 hours at 78 ℃, and obtaining acylchlorinated graphene oxide after the reaction is finished; uniformly mixing acyl chloride graphene oxide, undecylenol, swollen konjac glucomannan, triethylamine, toluene and dimethylacetamide, stirring and reacting at 65 ℃ for 70 hours, carrying out solid-liquid separation, washing and drying after the reaction is finished, adding the obtained product into water, adding 1-hexadecyl-3-methylimidazole bromide and didodecyldimethylammonium bromide, carrying out ultrasonic dispersion, and stirring at 60 ℃ for 12 hours to obtain the modified konjac glucomannan; wherein the weight ratio of the graphene oxide to the sodium hydroxide to the sodium chloroacetate is 36: 2.4: 5; the weight volume ratio of the carboxylated graphene oxide to the dimethyl formamide to the thionyl chloride is 58 g: 20 ml: 40 ml; the weight ratio of the acyl chloride graphene oxide to the undecylenol to the konjac glucomannan to the triethylamine to the toluene to the dimethylacetamide is 1: 1: 7: 1: 9: 7; the total weight of the 1-hexadecyl-3-methylimidazole bromide and the didodecyldimethylammonium bromide is 0.15 times of the weight of the product, and the weight ratio of the 1-hexadecyl-3-methylimidazole bromide to the didodecyldimethylammonium bromide is 1: 4.

comparative example 1

The only difference from example 5 is that: the raw materials do not contain rice husk charcoal.

Comparative example 2

The only difference from example 5 is that: the raw materials do not contain sodium humate.

Comparative example 3

The only difference from example 5 is that: the raw materials do not contain zeolite.

Comparative example 4

The only difference from example 5 is that: the raw materials do not contain modified konjac glucomannan.

Comparative example 5

The only difference from example 5 is that: the raw material contains unmodified konjac glucomannan, but does not contain the modified konjac glucomannan in example 5.

Comparative example 6

The only difference from example 5 is that: the modified konjac glucomannan in the raw materials is prepared by simply mixing the konjac glucomannan and graphene oxide.

Comparative example 7

The only difference from example 5 is that: the modified konjac glucomannan in the raw materials is konjac glucomannan esterified by sodium hexametaphosphate.

The detoxification agents of example 5 and comparative examples 1 to 7 were subjected to an adsorption test, which was carried out as follows:

preparing 80ml of zearalenone with the concentration of 14mg/L by using a phosphate buffer solution with the pH of 3.5, evenly dividing each 10ml into 8 parts, respectively adding 4mg of the detoxifying agent of the embodiment 5 and the detoxifying agents of the comparative examples 1-7, performing vibration adsorption for 60min at 30 ℃, filtering a supernatant through a filter membrane with the diameter of 0.25 mu m, detecting the concentration of the zearalenone in the supernatant by using high performance liquid chromatography, and calculating the adsorption rate, namely the detoxifying rate; it is found by testing and calculation that the detoxification rate of the detoxification agent of example 5 is 97.3%, and the detoxification rate of the detoxification agent of comparative examples 1-7 is 72.7-89.1%.

Taking residues obtained after detoxification of the detoxication agents in the example 5 and the comparative examples 1 to 7, respectively adding phosphate buffer solutions with the pH values of 2.0 and 8.0, oscillating and desorbing for 10 hours in a constant-temperature oscillating water bath kettle at the temperature of 60 ℃, then centrifuging, measuring the content of toxins in supernate, and calculating the desorption rate; it was tested and calculated that the desorption rate of the detoxifying agent of example 5 was 2.7% when the phosphate buffer solution having pH 2 was added, whereas the desorption rate of the detoxifying agent of comparative examples 1 to 7 was 4.6 to 7.9%, and the desorption rate of the detoxifying agent of example 5 was 3.5% when the phosphate buffer solution having pH 8 was added, whereas the desorption rate of the detoxifying agent of comparative examples 1 to 7 was 6.2 to 8.7%.

The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.