阅读说明：本技术 防治禽类禽流感的复方黄芪多糖可溶性粉 (Compound astragalus polysaccharide soluble powder for preventing and treating avian influenza ) 是由 杨成祥 赵云生 赵蕾 于 2021-10-28 设计创作，主要内容包括：本发明涉及防治禽类禽流感的复方黄芪多糖可溶性粉,可有效解决禽类禽流感用药的问题,其解决的技术方案是,是由以下重量计的：黄芪多糖550～650g、牛膝多糖180～220g、猫爪草多糖110～130g和木鳖子霜75～85g作原料药物,再添加原料药物重量3%的辅料制备成可溶性粉,所述的辅料为糖粉、糊精,按重量比4:1混配均匀、粉碎,在60℃下干燥、过80目筛而成。本发明原料丰富,价格低廉,用药安全,无毒副作用,使用效果好,是防治禽类禽流感的中兽药物的创新。(The invention relates to compound astragalus polysaccharide soluble powder for preventing and treating avian influenza, which can effectively solve the problem of medication of the avian influenza and adopts the technical scheme that the soluble powder is prepared from the following components in parts by weight: 550-650 g of astragalus polysaccharide, 180-220 g of achyranthes polysaccharide, 110-130 g of ternate buttercup root polysaccharide and 75-85 g of momordica cream are used as raw material medicaments, and auxiliary materials accounting for 3% of the weight of the raw material medicaments are added to prepare soluble powder, wherein the auxiliary materials are powdered sugar and dextrin, and the soluble powder is prepared by uniformly mixing and crushing the powdered sugar and the dextrin according to the weight ratio of 4:1, drying the powdered sugar and the dextrin at 60 ℃ and sieving the powdered sugar and the dextrin by a sieve of 80 meshes. The veterinary drug has the advantages of rich raw materials, low price, safe medication, no toxic or side effect and good use effect, and is an innovation of the veterinary drug for preventing and treating avian influenza of poultry.)

1. A compound astragalus polysaccharide soluble powder for preventing and treating avian influenza is characterized by comprising the following components in parts by weight: 550-650 g of astragalus polysaccharide, 180-220 g of achyranthes polysaccharide, 110-130 g of ternate buttercup root polysaccharide and 75-85 g of momordica cream are used as raw material medicaments, and auxiliary materials accounting for 3 percent of the weight of the raw material medicaments are added to prepare soluble powder, and the operation steps are as follows:

step (1): the astragalus polysaccharide is dried astragalus, water is added to the dried astragalus, extraction is carried out for three times, the first extraction is carried out, the astragalus polysaccharide is firstly soaked in water with the weight being 12 times of that of the astragalus for 30-60 min, the mixture is boiled and boiled with slow fire, then the mixture is boiled with slow fire for 1.5 h, filtration is carried out to obtain second filtrate, the mixture is added with water with the weight being 10 times of that of the astragalus to carry out third extraction, the mixture is boiled with slow fire for 1 h, filtration is carried out to obtain third filtrate, the three filtrates are combined, concentrated to be 1:1 concentrated solution, absolute ethyl alcohol with the volume being 4 times of that of the concentrated solution is used for precipitation, and precipitates are dried to obtain the astragalus polysaccharide;

step (2): the achyranthes bidentata polysaccharide is prepared by leaching dried achyranthes bidentata for 2 hours at 100 ℃ in a material-liquid ratio of 1:15 for 2 times by adding ethanol with the mass concentration of 95% which is 5 times of the weight of the achyranthes bidentata into leaching liquor, leaching for 2 times, each time for 2 hours, combining filtrates, concentrating and drying to obtain the achyranthes bidentata polysaccharide;

and (3): the radix ranunculi ternati polysaccharide is prepared by leaching 2 times of radix ranunculi ternati with water, wherein the added water amount is 12 times of the weight of the radix ranunculi ternati, leaching 3 hours each time, combining leaching liquor obtained in two times, concentrating to obtain concentrated solution containing 800g/L of crude drug, adding absolute ethyl alcohol with the volume of 50% of the concentrated solution, standing for 6 hours, discarding upper liquid to obtain precipitate, and drying the precipitate to obtain the radix ranunculi ternati polysaccharide;

and (4): the momordia momordica cream is prepared by pressing momordia momordica kernels in an extrusion type cream making machine, controlling the temperature to be 100 ℃, stopping squeezing when the oil output reaches 17-20%, and crushing and uniformly mixing the medicine residues to obtain the momordia momordica cream;

and (5): mixing the above Astragalus polysaccharides, achyranthes polysaccharides, radix Ranunculi Ternati polysaccharides, and Amyda sinensis cream, drying, mixing the adjuvants including sugar powder and dextrin, pulverizing, drying at 60 deg.C, and sieving with 80 mesh sieve.

2. The compound astragalus polysaccharide soluble powder for preventing and treating avian influenza of poultry according to claim 1 is characterized by comprising the following components in parts by weight: 550g of astragalus polysaccharide, 180g of achyranthes polysaccharide, 110g of ternate buttercup root polysaccharide and 75g of momordica cream are used as raw material medicaments, auxiliary materials accounting for 3 percent of the weight of the raw material medicaments are added to prepare soluble powder, and the auxiliary materials are powdered sugar and dextrin which are uniformly mixed and crushed according to the weight ratio of 4:1, dried at the temperature of 60 ℃ and sieved by a sieve of 80 meshes.

3. The compound astragalus polysaccharide soluble powder for preventing and treating avian influenza of poultry according to claim 1 is characterized by comprising the following components in parts by weight: 600g of astragalus polysaccharide, 200g of achyranthes polysaccharide, 120g of ternate buttercup root polysaccharide and 80g of momordica cream are used as raw material medicaments, auxiliary materials accounting for 3 percent of the weight of the raw material medicaments are added to prepare soluble powder, and the auxiliary materials are powdered sugar and dextrin which are uniformly mixed and crushed according to the weight ratio of 4:1, dried at the temperature of 60 ℃ and sieved by a sieve of 80 meshes.

4. The compound astragalus polysaccharide soluble powder for preventing and treating avian influenza of poultry according to claim 1 is characterized by comprising the following components in parts by weight: 650g of astragalus polysaccharide, 220g of achyranthes polysaccharide, 130g of ternate buttercup root polysaccharide and 85g of momordica cream are used as raw material medicaments, auxiliary materials accounting for 3 percent of the weight of the raw material medicaments are added to prepare soluble powder, the auxiliary materials are powdered sugar and dextrin, the powdered sugar and the dextrin are uniformly mixed and crushed according to the weight ratio of 4:1, the mixture is dried at 60 ℃, and the mixture is sieved by a 80-mesh sieve.

Technical Field

The invention relates to veterinary medicine and pharmaceutical technology, in particular to compound astragalus polysaccharide soluble powder for preventing and treating avian influenza of poultry.

Background

The avian influenza virus is mainly infected by poultry, but in recent years, especially the highly pathogenic avian influenza virus H5N1 can cause infection and death of various mammals, for example, the infection and the death of tigers and leopard avian influenza occur in zoos in China and Tiger and dog in Thailand zoos, which causes mass death, and the infection and the death of other animals such as pigs, cats, dogs and the like are reported. Of course, avian influenza virus infection in many mammals is the most harmful to humans as avian influenza infection in swine. Once pigs are co-infected with human and avian influenza viruses, variant strains that are transmitted and circulating between humans are likely to be produced. Research shows that the HA, NA and PB1 genes of the H2N2 subtype virus epidemic in Asia from 1957 to 1968 are all from avian influenza virus, and other genes keep the genes of the H1N1 virus epidemic in people at the time; the H3N2 subtype virus prevalent in hong kong in 1968, its HA and PB1 were also from avian influenza virus, and other genes were from H2N2 virus prevalent in the population at that time. That is, viruses causing two human influenza pandemics are both hybrid viruses of human strains and avian strains. Since pigs play an important role in the formation of this hybrid virus, they are considered "mixers". In addition, after a period of adaptation in vivo after infection of mammals with avian influenza virus, the receptor specificity may change, and it may also become a strain that can infect humans.

From the infection and the morbidity of mammals at present, animals such as tigers, leopards and cats in felines and mink animals such as ferrets, minks and stone minks in ferrets are very sensitive to avian influenza viruses, and the ferrets are also ideal experimental animals of the influenza viruses. The important status of minks on the special economic animal breeding in China and the fact that highly pathogenic avian influenza continuously occurs in China are combined, and the minks are reminded that high attention must be paid to the occurrence and influence of the mink avian influenza.

Genetics and variation are fundamental characteristics of any organism; especially under the adverse environmental stress, the variation is promoted, for example, the stress of the antibody can cause the change of the antigenicity of the virus. In China, the application of H9N2 subtype avian influenza vaccine has been for at least 10 years, while the current epidemic H9 subtype avian influenza virus has at least 3 groups of different types of antigen groups, and the cross reactivity among different antigen groups is poor; the application of the H5 subtype influenza vaccine has a certain time, according to the existing reported data, the antigenicity of highly pathogenic avian influenza hemagglutinin in Guangxi, Yunnan, Jiangxi, Hunan and other places of China has larger difference, and animal experiments show that the virus inoculated to ducks and the virus discharged in the later period have different number of amino acid differences compared with the original inoculated virus, which fully shows that the H5 subtype avian influenza virus antigen is easy to generate variation, and the variation of the avian influenza virus antigen seems to enter an active period. Thus, alteration of the antigenicity of avian influenza viruses poses great difficulties both in the immunization of animals and in the vaccination of humans.

The existing prevention and treatment medicines for avian influenza are deficient: western medicines such as amantadine and the like have been used for decades as medicines for treating influenza of people and livestock and poultry. However, human H3N1, H3N2 have produced strains resistant to amantadine. Recently, it has been reported that some people use "tamiflu" to start generating new strains, which is very concerned, but it mainly appears in human. The western medicine for resisting virus and preventing and treating the avian influenza is not allowed in China. According to the current veterinary drug management regulations, administrative penalties are given as long as western antiviral drugs such as amantadine and the like are found to be used for veterinary drug sales and production. Therefore, no antiviral veterinary drug is available in the aspect of preventing and treating avian influenza of poultry at present.

Part of farmers still adopt western antiviral drugs such as amantadine and the like to prevent and treat avian influenza in violation of national regulations:

firstly, after poultry are infected with avian influenza, an immune defense system of an organism is firstly destroyed, so that the resistance and self-repairing capability of the organism are lost;

secondly, the western antiviral medicines have partial antagonism on virus particles after being clinically used, but also have toxic and side effects on body cells of the poultry, so the veterinary clinical western antiviral medicines have not ideal effect on preventing and treating the avian influenza of the poultry;

and thirdly, serious problems of food safety and the like can be caused after western medicine antiviral drugs are used in the poultry raising process, so that the health of human beings and the prevention and control safety of human diseases are influenced.

In the clinical prevention and treatment research and exploration of avian influenza by human beings, the clinical effects of part of Chinese veterinary drug varieties such as astragalus, momordica cochinchinensis and the like for preventing and treating avian influenza of poultry are found to be good, and the research also finds that the Chinese veterinary drug such as astragalus and the like not only has stronger clinical antiviral capability, but also has the effects of enhancing the immunity and disease resistance of the organism and improving the self-repairing capability of the organism.

The traditional Chinese veterinary medicine is relatively simple in processing technology and relatively rough in processing, and is mostly prepared from simple coarse powder or fine powder or is directly mixed into poultry feed or drinking water after being decocted traditionally, so that the effective ingredients of the traditional Chinese veterinary medicine cannot be sufficiently and effectively absorbed and utilized, and the clinical application effect is not ideal. Therefore, improvement of drugs for preventing and treating avian influenza is a problem which needs to be solved urgently at present.

Disclosure of Invention

In order to solve the defects of the prior art, the invention aims to provide the compound astragalus polysaccharide soluble powder for preventing and treating avian influenza, which can effectively solve the problem of drug administration of avian influenza.

The technical scheme of the invention is that the material is prepared from the following components by weight: 550-650 g of astragalus polysaccharide, 180-220 g of achyranthes polysaccharide, 110-130 g of ternate buttercup root polysaccharide and 75-85 g of momordica cream are used as raw material medicines, and auxiliary materials accounting for 3 percent of the weight of the raw material medicines are added to prepare granules, the astragalus polysaccharide is dried astragalus, water is added for leaching for three times, the first leaching is carried out, water with the weight being 12 times of that of the astragalus is firstly used for soaking for 30-60 min, the mixture is boiled and then boiled for 1.5 hours by slow fire, filtering to obtain first filtrate, adding water 10 times of radix astragali into the residue, extracting for the second time, boiling, decocting with slow fire for 1.5 hr, filtering to obtain a second filtrate, adding water 10 times the weight of radix astragali into the residue, extracting for the third time, boiling, decocting with slow fire for 1 hr, filtering to obtain a third filtrate, mixing the three filtrates, concentrating to 1:1 concentrate, precipitating with 4 times of anhydrous ethanol, and drying the precipitate to obtain Astragalus polysaccharides; the achyranthes bidentata polysaccharide is prepared by leaching dried achyranthes bidentata for 2 hours at 100 ℃ in a material-liquid ratio of 1:15 for 2 times by adding ethanol with the mass concentration of 95% and the weight of 5 times of that of achyranthes bidentata into a leaching solution, leaching for 2 times, each time for 2 hours, combining filtrates, concentrating and drying to obtain the achyranthes bidentata polysaccharide; the radix ranunculi ternati polysaccharide is prepared by leaching 2 times of radix ranunculi ternati with water, wherein the added water amount is 12 times of the weight of the radix ranunculi ternati, leaching 3 hours each time, combining leaching liquor obtained in two times, concentrating to obtain concentrated solution containing 800g/L of crude drug, adding absolute ethyl alcohol with the volume of 50% of the concentrated solution, standing for 6 hours, discarding upper liquid to obtain precipitate, and drying the precipitate to obtain the radix ranunculi ternati polysaccharide; the momordia momordica seed cream is prepared by putting momordia momordica seed into an extrusion type cream making machine for pressing, controlling the temperature to be 100 ℃, stopping pressing when the oil content reaches 17-20%, and crushing and uniformly mixing the medicine residues to obtain the momordia momordica seed cream; mixing the above Astragalus polysaccharides, achyranthes polysaccharides, radix Ranunculi Ternati polysaccharides, and semen Momordicae cream, drying, mixing the adjuvants including sugar powder and dextrin, pulverizing, drying at 60 deg.C, and sieving with 80 mesh sieve.

The invention has the advantages of rich raw materials, low price, safe medication, no toxic or side effect and good use effect, and is an innovation of traditional Chinese veterinary medicines for preventing and treating avian influenza.

Detailed Description

The following examples further illustrate the embodiments of the present invention in detail.

Example 1:

the invention, when embodied, may be made from the following by weight: 550g of astragalus polysaccharide, 180g of achyranthes polysaccharide, 110g of ternate buttercup root polysaccharide and 75g of momordica cream are used as raw material medicaments, the raw materials are mixed and dried, auxiliary materials accounting for 3 percent of the weight of the raw material medicaments are added, the auxiliary materials are powdered sugar and dextrin which are fully mixed and crushed according to the ratio of 4:1, the mixture is dried at the temperature of 60 ℃, and the mixture is sieved by a sieve with 80 meshes, so that the compound astragalus polysaccharide soluble powder is obtained.

Example 2:

the invention, when embodied, may be made from the following by weight: 600g of astragalus polysaccharide, 200g of achyranthes polysaccharide, 120g of ternate buttercup root polysaccharide and 80g of momordica cream are used as raw material medicaments, the raw materials are mixed and dried, auxiliary materials accounting for 3 percent of the weight of the raw material medicaments are added, the auxiliary materials are powdered sugar and dextrin which are fully mixed and crushed according to the ratio of 4:1, the mixture is dried at the temperature of 60 ℃, and the mixture is sieved by a 80-mesh sieve, so that the compound astragalus polysaccharide soluble powder is obtained.

Example 3:

when the invention is implemented, the invention can also be prepared by the following weight portions: 650g of astragalus polysaccharide, 220g of achyranthes polysaccharide, 130g of ternate buttercup root polysaccharide and 85g of momordica cream are used as raw material medicaments, the raw materials are mixed and dried, auxiliary materials accounting for 3 percent of the weight of the raw material medicaments are added, the auxiliary materials are powdered sugar and dextrin which are fully mixed and crushed according to the ratio of 4:1, the mixture is dried at the temperature of 60 ℃, and the mixture is sieved by a sieve with 80 meshes, so that the compound astragalus polysaccharide soluble powder is obtained.

Clinical trial example 1:

clinical test of compound astragalus polysaccharide soluble powder on avian influenza

1 materials and methods

1.1 test materials

The compound astragalus membranaceus soluble powder; amantadine is available from pharmaceutical chemicals, some Beijing.

1.2 test Chicken selection and grouping

Experiments arrange that 3 closed chicken houses in different disease stages in a certain chicken farm are subjected to AGP and HI tests on a disease material and a blood sample, bacterial culture, virus separation and clinical symptom combination and pathological dissection to confirm that the subtype of the Avian Influenza (AI) is H9. 3036 Toosendan, totally randomly divided into 1520 test groups and 1516 control groups; the Yidong 3057 is completely randomly divided into 1530 of the test group and 1527 of the control group; third, 3086, which are completely randomly divided into 1540 test groups and 1546 control groups. All 3 tested chickens were 28-week old roman brown commercial generation laying hens. The first-span chicken flock is the 3 rd day old of the disease, the second-span chicken flock does not have symptoms (is in the latent period), and the third-span chicken flock is the 14 th day old after the disease.

1.3 Breeding management

And each house carries out feeding management according to a normal feeding management method, the feeding management conditions of each house are the same, and the management methods of each test group and the corresponding control group are also the same.

1.4 protocol

The control group uses 160mg/L amantadine to drink water, the test group uses compound astragalus polysaccharide soluble powder to drink water, the dosage of the test A is 200mg/L, the dosage of the test B and the test C is 160mg/L, and the continuous medication is 14 d. The conditions of the chickens in each group, such as spirits, ingestion, drinking, feces, egg laying number, morbidity, mortality and the like, are observed and recorded every day. In this trial, the second group started to develop disease on day 4 of the trial, and the control group of the third group relapsed to the avian influenza H9 subtype on day 6 of the trial, i.e., 20 days after the initial onset.

2 results and analysis

The test results are shown in Table 1. The test results show that the laying rate level of each test group is equivalent to that of a control group (P is greater than 0.05) at the beginning of the test (P), each test group is obviously superior to the corresponding control group on all indexes in the test period, and compared with the corresponding control group, the first-stage traditional Chinese medicine preparation has the same morbidity, the second-stage traditional Chinese medicine preparation has significant difference (P is less than 0.05), and the third-stage traditional Chinese medicine preparation has very significant difference (P is less than 0.01); the difference between the first and second plots is not significant (P >0.05) and the difference between the third plots is extremely significant (P <0.01) in the mortality rate; the differences of the first, second and third plants in the laying rate reduction range are very obvious (P is less than 0.01); the differences of the first, second and third strains of the egg are obvious in the malformed egg rate (such as soft-shell eggs, etc.) (P is less than 0.05).

TABLE 1 test results

3 conclusion

The results of the prevention and treatment test are combined with the local epidemic characteristics of the disease, so that the following conclusions can be drawn:

3.1 the compound astragalus polysaccharide soluble powder is applied to the prevention and treatment in the front, middle and back 3 stages of the avian influenza, the mortality of the chicken and the reduction range of the laying rate can be greatly reduced, and the compound astragalus polysaccharide soluble powder has obvious antiviral and anti-inflammatory effects; is obviously superior to amantadine;

3.2 the tolerant chicken group with primary disease obviously can obtain certain immunity to the disease, but because the disease destroys the immune system of the chicken, a plurality of chicken groups can relapse the disease to different degrees after the initial 20-30 days, and if the compound astragalus polysaccharide soluble powder is added after the initial disease, the relapse of the disease can be effectively prevented;

3.3 the use of the compound astragalus polysaccharide soluble powder for preventing and treating the disease is obviously superior to amantadine in the aspects of reducing the laying rate and recovering the eggshell quality;

3.4 the traditional Chinese medicine considers that the astragalus has the drug effects of tonifying middle-jiao and Qi, strengthening exterior and invigorating yang, promoting tissue regeneration, and strengthening interior and consolidating foundation. The compound astragalus polysaccharide soluble powder is prepared by refining and extracting traditional Chinese medicine astragalus, the main components of the compound astragalus polysaccharide soluble powder are various immunity enhancing substances such as astragalus polysaccharide I, astragalus polysaccharide II and the like, and the compound astragalus polysaccharide soluble powder is matched with achyranthes polysaccharide, ternate buttercup root polysaccharide and momordica cochinchinensis cream to support each other, so that the compound astragalus polysaccharide soluble powder can stimulate an organism to generate a large amount of leukocyte interferon, phagocytes and lymphocytes; but also can strengthen the humoral immunity path in vivo, generate a large amount of immunoglobulin and complement, improve microcirculation, effectively condition and enhance the humoral immunity and cellular immunity of the organism, improve the immune function of the organism, generate interference, phagocytosis and combination effects on viruses, kill the viruses and bacteria in various ways, have the effects of clearing away heat and toxic materials and regulating the metabolic dysfunction of the organism, and enhance the disease resistance of poultry.

Functional summary example 1:

the efficacy of the compound astragalus polysaccharide soluble powder

The compound astragalus polysaccharide soluble powder has the following effects: has the functions of clearing away heat and toxic material, strengthening body resistance, eliminating evil, invigorating spleen and kidney, benefiting vital energy, nourishing blood, resisting virus and bacteria, inducing body to produce interferon, promoting antibody formation, strengthening body's immunity and repairing capacity of various systems, resisting disease, promoting growth, resisting stress, etc. Especially has the best effect of preventing and treating avian influenza of poultry.

1. Broad spectrum antiviral applications: the compound astragalus polysaccharide soluble powder is a broad-spectrum antiviral and interferon inducer, and can stimulate the functions of macrophages and T cells, increase the number of E-ring forming cells, induce cytokines and promote the induction of interleukins. So that the animal body can produce endogenous interferon, thereby achieving the aim of resisting virus. Can be used for treating viral infectious diseases such as avian influenza and infectious bursal disease. Radix astragali can prevent common cold and reduce incidence rate by more than 80%, and radix astragali polysaccharide can reduce incidence rate by more than 90% when combined with Achyranthis radix polysaccharide, radix Ranunculi Ternati polysaccharide and semen Momordicae cream.

2. The application of enhancing the immunity of the vaccine comprises the following steps: the compound astragalus polysaccharide soluble powder has the functions of enhancing the name epidemic capacity of organisms, greatly enhancing the immunity of vaccine, having good protection effect on vaccine, and according to research reports that the astragalus polysaccharide can enhance the serum ramet titer of Marek's disease vaccine. The compound astragalus polysaccharide soluble powder can be used together with NDV and IV vaccines to improve the serum HI antibody titer of immunized chickens, and can be matched with the compound astragalus polysaccharide soluble powder to enhance the chicken coccidium immunity protection when coccidium vaccines are used.

3. Effects on the liver: the compound astragalus polysaccharide soluble powder can promote the synthesis of DNA and RNA of liver cells, relieve pathological changes caused by hepatotoxic substances, enhance the detoxification function of the liver and protect the liver cells. Has good prevention and treatment effects on chicken fatty liver, hydropericardium hepatitis syndrome, hepatosplenopathy and hepatitis splenomegaly syndrome, and can improve the feed intake of livestock and poultry, thereby promoting the growth and development of livestock and poultry.

4. Has the effects on the kidney: the compound astragalus polysaccharide soluble powder can improve renal cell metabolism and renal function, reduce blood creatinine level and relieve renal failure after nephrectomy. Has good prevention and treatment effects on nephrotic syndrome, acute renal failure, nephrotic syndrome, kidney swelling, urate deposition and other symptoms. Meanwhile, the kidney-invigorating function can be achieved, and the growth and breeding functions of livestock and poultry can be exerted.

5. The reproductive performance is improved: the compound astragalus polysaccharide soluble powder has the functions of stabilizing cells, resisting aging, resisting free radicals and peroxidation, and can obviously improve the service life and the use efficiency of breeding hens after long-term regular use. In addition, the compound astragalus polysaccharide soluble powder has the effect of gonadotropin, and can thicken endometrium, enlarge uterine cavity and increase the weight of uterine horn and ovary of female animals. The compound astragalus polysaccharide soluble powder can also improve the reproductive performance of male animals, and can obviously improve the activity of sperms, the survival rate of the sperms, the density of the sperms and the like.

6. Is the fourth need for chick health care: currently, young bird health care is mostly made in three aspects: the vaccine for preventing the virus disease is prepared; antibiotics for preventing bacterial diseases; and the nutrient substances for enhancing the physique and improving the immunity. Through long-term practical research, the 'fourth requirement' of the young poultry health care is found to be the compound astragalus polysaccharide soluble powder while the three health care requirements of the young poultry are met. It not only can fully play the three health-care functions, but also can meet the requirements of health-care of young poultry, no drug residue and full play of the growth potential of young poultry.

7. Has the functions of improving the utilization of nutrient substances and promoting the growth of animals. The compound astragalus polysaccharide soluble powder contains various nutrient substances such as amino acid, vitamin, trace elements and the like, and Unknown Growth Factors (UGF). The feed additive can obviously improve the growth speed of livestock and poultry, improve the body resistance and improve the quality and the yield of eggs.

The invention has obvious curative effect in clinical application, and is better applied to the clinical applicant to carry out mouse acute toxicity experiment and rat long-term toxicity experiment according to the registered and reported data requirements in the New drug registration management method. The test result shows that the compound astragalus polysaccharide soluble powder has small acute toxicity to mice, and the application of a large dose of the preparation in one day in clinic can not generate toxic reaction. The long-term oral administration to rats has lower toxicity and better safety.

The components of the invention use astragalus polysaccharide, achyranthes polysaccharide, radix ranunculi ternati polysaccharide and momordica cochinchinensis cream to be scientifically and reasonably compatible and mutually supported, and the invention is effectively used for treating bird flu and the like, has good curative effect and safe medication and is fully proved by experiments.

The formula of the invention comprises:

astragalus root: is root of Astragalus membranaceus (Fisch.) Bge of Leguminosae family or Astragalus membranaceus (Fisch.) Bge. Mainly produced in inner Mongolia, Shanxi, Heilongjiang, etc. Medicine property: sweet and warm. It enters spleen and lung meridians. The efficacy is as follows: invigorating qi, invigorating spleen, invigorating yang, lifting body, consolidating superficial resistance, inducing diuresis, relieving swelling, removing toxic materials, and promoting granulation. Modern research: mainly contains glycosides, polysaccharides, flavones, amino acids, trace elements, etc. The astragalus can promote the metabolism of organisms, resist fatigue and promote the renewal of serum and liver proteins; has obvious diuretic effect, and can eliminate urine protein of experimental nephritis; can improve anemic animal phenomena; can raise hypoglycemia; the respiration can be excited; can enhance and regulate body immunity, promote interferon system, and improve body resistance; the compound preparation has slight inhibition effect on cytopathic effect caused by various viruses such as influenza virus and the like, and has protective effect on mice infected by the influenza virus; has wide antibacterial effect; the astragalus can obviously increase the cell number, promote the growth of cells and prolong the service life in cell culture; can enhance myocardial contractility, protect cardiovascular system, resist arrhythmia, dilate coronary artery and peripheral blood vessel, lower blood pressure, reduce platelet adhesion, and reduce thrombosis; it also has effects in reducing blood lipid, resisting aging, resisting anoxia, resisting radiation, and protecting liver. In recent years, studies have found that Astragalus contains various active ingredients such as polysaccharides, saponins, flavones and amino acids, and the active ingredients all have the effect of promoting antibody production and immune reaction, and most of the studies on Astragalus Polysaccharides (APS) are reported. The astragalus polysaccharide is one of the main active ingredients of astragalus and is obtained by high-tech extraction and separation. Astragalus polysaccharides are the main components of astragalus root.

Achyranthes root: is root of Achyranthes bidentata (Achyranthes bidentata) Achyranthus bidenta Bl. and Cyathura officinalis Kuan (sweet Achyranthes bidentata). Medicine property: bitter, sweet, sour and neutral. It enters liver and kidney meridians. The efficacy is as follows: promoting blood circulation, dredging channels, nourishing liver and kidney, strengthening tendons and bones, inducing diuresis, treating stranguria, and inducing fire (blood) to descend. Modern research: radix Achyranthis contains triterpenoid saponin (prepared by hydrolyzing to oleanolic acid and sugar), ecdysterone, inocosterone, and radix Achyranthis bidentatae sterone, and polysaccharides. In addition, Achyranthis radix also contains 12 kinds of amino acids such as arginine, alkaloids, coumarins, and trace elements such as iron and copper. The total saponins of achyranthes bidentata have obvious exciting effect on uterine smooth muscle, the achyranthes bidentata benzene extract has obvious antifertility, anti-implantation and anti-early pregnancy effects, and the active ingredient for antifertility is ecdysteroid. The achyranthes bidentata alcohol extract has an inhibiting effect on the heart of a test small animal, and the decoction has an inhibiting effect on the cardiac muscle of an anaesthetized dog. The decoction and the alcohol extract have transient blood pressure lowering and mild diuretic effects, and are accompanied with respiratory excitation. Achyranthes bidentata can reduce the whole blood viscosity, hematocrit and erythrocyte aggregation index of rats and has anticoagulation effect. The ecdysterone has effects of reducing blood lipid and lowering blood sugar. Achyranthis radix has antiinflammatory, analgesic, and immunity enhancing effects.

Radix ranunculi ternati: is root tuber of Ranunculaceae Ranunculus runcinatus Thunb. Medicine property: sweet, pungent and slightly warm. It enters liver and lung meridians. The efficacy is as follows: resolve phlegm and dissipate nodulation, remove toxicity and relieve swelling. Modern research: ranunculus ternatus thumb contains ranunculalin, protoanemonin, arachidic acid, octadecyl myristate, stigmasterol, beta-sitosterol, glucose, arabinose, galactose, and oil and plant alkaloid. Has broad-spectrum antibacterial, antitumor, antiinflammatory, and protective inhibiting effects.

Semen momordicae: is mature seed of Momordica cochinchinensis (Lour.) Spreng. Medicine property: bitter, slightly sweet and cool. Is toxic. It enters liver, spleen and stomach meridians. The efficacy is as follows: counteract toxic pathogen and cure sore, resolve swelling and dissipate nodulation. Modern research: contains momordica saponin, momordica acid, momordica element, oleanolic acid, sterol, amino acid, 35.72% of oil, 30.59% of protein, trehalose, etc. The semen Momordicae saponin has antiinflammatory and blood pressure lowering effects, and can inhibit isolated frog heart and isolated rabbit duodenum. The water extract, ethanol-water extract and ethanol extract have blood pressure lowering effect when tested in anesthetized animals such as dog, cat and rabbit. However, the toxicity is high, and animals die within days regardless of intravenous or intramuscular injection. The rats are injected with momordica cochinchinensis saponin intravenously, the blood pressure is temporarily reduced, the breath is excited temporarily, and the heart beat is accelerated. The injection is injected into dog femoral artery for temporarily increasing blood flow of lower limb, and the action intensity is 1/8 of papaverine. It has inhibitory effect on ex vivo frog heart and ex vivo rabbit duodenum, and has acetylcholine enhancing and papaverine antagonizing effects on guinea pig ileum, and causes irreversible contraction at high concentration. The momordica saponin is orally taken or subcutaneously injected to rats, and can obviously inhibit the foot edema caused by carrageenan. Has hemolytic effect on rabbit erythrocyte. Saponin of seeds: 6.6mg/kg can cause transient respiratory excitation and blood pressure reduction when being injected into a rat vein, can increase the blood flow of hind limb peripheral blood vessels of dogs and has inhibiting effect on isolated frog heart; the concentration of 2 x 10 (-4) g/ml can increase the excitation effect of acetylcholine on guinea pig ileum; the injection has effect in inhibiting swelling and inflammation of rat foot caused by carrageenan; has hemolytic effect; half of the lethal dose of the mice was injected intravenously at 32.35mg/kg and intraperitoneally at 37.34 mg/kg. Semen Momordicae extract has blood pressure lowering effect on anesthetized dog, cat and rabbit, and intravenous injection of semen Momordicae saponin into rat can temporarily lower blood pressure, accelerate heart beat, and excite breath transiently. The injection is injected into dog femoral artery for temporarily increasing blood flow of lower limb, and the action intensity is 1/8 of papaverine. In addition, has antiinflammatory and hemolytic effects.

Clinical application example 1:

in a word, the compound astragalus polysaccharide soluble powder of the invention has the following components:

1. the composition is used for preventing and treating low-pathogenicity avian influenza of poultry and effectively preventing and controlling immunity of high-pathogenicity avian influenza;

2. effectively treating and preventing the immunologic hypofunction of the poultry (atrophy of organs such as thymus, liver, kidney, bursa of fabricius and the like);

3. the feed has special effects on slow growth speed of meat poultry, low feed intake, poor uniformity, no egg laying peak of egg-laying poultry or egg drop caused by unknown reasons, refractory watery diarrhea, rectocele and the like;

4. the product has protective and synergistic effects on vaccine, obviously improves the immunity potency, and can be used together with vaccine;

5. the product can be used together with anticoccidial drug to improve anticoccidial index;

6. preventing and treating various viral diseases of livestock and fowl, such as circovirus, porcine reproductive and respiratory syndrome, influenza, pseudorabies, viral diarrhea, recurrent asthma, and innominate hyperpyrexia;

7. the clinical application effect is good, the market of soluble powder is scarce, and the soluble powder has higher competitiveness than popular powder, high technological content and high added value;

8. the soluble powder process has distinct advantages, is convenient for storage, transportation and clinical application, and is easier to cut into the market;

9. stable character, excellent water solubility, no toxicity, no harm and no medicine residue;

10. the drinking water or the mixing material is convenient and uniform to use, and the product development prospect is wide.

After years of research, clinical tests and practical summary, the compound astragalus polysaccharide soluble powder is feasible and effective, is worthy of popularization, and is effectively used for preventing and treating virus diseases such as bird flu and the like.